Claims
- 1. A process for decolorizing E 1 effluent which comprises treating said effluent with the Ligninolytic Mixture.TM. from a Phanerochaete chrysosporium fermentation consisting essentially of rLDM.TM. 1 through 6, and other ligninolytic enzymes.
- 2. A process, according to claim 1, wherein said Phanerochaete chrysosporium is the novel mutant strain designated SC26, having the identifying characteristics of NRRL 15978.
- 3. A process for decolorizing E1 effluent which comprises treating said effluent with enzymes consisting essentially of rLDM.TM. 1 through 6, individually or with mixtures thereof.
- 4. A process, according to claim 1, wherein said Ligninolytic Mixture.TM. from a fermentation of Phanerochaete chrysosporium is added to a solution of about 0.01 to about 20% E1 effluent, the effluent being in about 2 .mu.M to about 10 mM of H.sub.2 O.sub.2, and buffered at about pH 2 to about pH 7: the solution is then incubated with shaking at about 15.degree. C. to about 50.degree. C. for about 2 min to about 48 hr to obtain decolorized effluent.
- 5. A process, according to claim 1, wherein said Ligninolytic Mixture.TM. from a fermentation of Phanerochaete chrysosporium is added to a solution of about 0.2% E1 effluent; the effluent is in about 400 .mu.M of H.sub.2 O.sub.2 and is buffered at about pH 4.5; the solution is then incubated with shaking at about 39.degree. C. for about 12 hr to obtain decolorized effluent.
- 6. A process, according to claim 1, wherein about 10 to about 500 .mu.M MnSO.sub.4 is added with the Ligninolytic Mixture.TM..
- 7. A process, according to claim 2, wherein about 10 to about 500 .mu.M MnSO.sub.4 is added with the Ligninolytic Mixture.TM..
- 8. A process, according to claim 6, wherein the concentration of MnSO.sub.4 is about 100 .mu.M.
- 9. A process, according to claim 7, wherein the concentration of MnSO.sub.4 is about 100 .mu.M.
- 10. A process, according to claim 4, wherein said Phanerochaete chrysosporium is the novel mutant strain designated SC26, having the identifying characteristics of NRRL 15978.
- 11. A process, according to claim 5, wherein said Phanerochaete chrysosporium is the novel mutant strain designated SC26, having the identifying characteristics of NRRL 15978.
- 12. A process for decolorizing E1 effluent which comprises treating said effluent with the extracellular growth medium from a Phanerochaete chrysosporium fermentation consisting essentially of rLDM.TM. 1 through 6, and other ligninolytic enzymes.
- 13. A process, according to claim 12, wherein said Phanerochaete chrysosporium is the novel mutant strain designated SC26, having the identifying characteristics of NRRL 15978.
- 14. A process, according to claim 12, wherein said extracellular growth medium from a fermentation of Phanerochaete chrysosporium is added to a solution of about 0.01 to about 20% E1 effluent, the effluent being in about 2 .mu.M to about 10 mM of H.sub.2 O.sub.2, and buffered at about pH 2 to about pH 7; the solution is then incubated with shaking at about 15.degree. C. to about 50.degree. C. for about 2 min to about 48 hr to obtain decolorized effluent.
- 15. A process, according to claim 12, wherein about 10 to about 500 .mu.M MnSO.sub.4 is added with the extracellular growth medium.
- 16. A process, according to claim 14, wherein said Phanerochaete chrysosporium is the novel mutant strain designated SC26, having the identifying characteristics of NRRL 15978.
- 17. A process for decolorizing E1 effluent which comprises treating said effluent with one or more of the following: rLDM.TM. 1 through 6, individually or a mixture thereof; Ligninolytic Mixture.TM. from a fermentation of Phanerochaete chrysosporium consisting essentially of rLDM.TM. 1 through 6, and other ligninolytic enzymes; and extracellular growth medium from a fermentation of Phanerochaete chrysosporium comprising rLDM.TM. 1 through 6, and other ligninolytic enzymes.
- 18. A process, according to claim 17, wherein said Phanerochaete chrysosporium is the novel mutant strain designated SC26, having the identifying characteristics of NRRL 15978.
CROSS REFERENCE TO A RELATED APPLICATION
This is a continuation-in-part of my copending application Ser. No. 755,245, filed Jul. 15, 1985, now abandoned.
Non-Patent Literature Citations (1)
Entry |
Alberti, B. N. and Klibanov, A. M., (1981), "Enzymatic Removal of Dissolved Aromatics from Industrial Aqueous Effluents", Biotechnology and Bioengineering Symp. 11:373-379. |
Continuation in Parts (1)
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Number |
Date |
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755245 |
Jul 1985 |
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