Claims
- 1. The use of fibroblastic cells as stem cells for cell differentiation, histiogenesis, organogenesis, cell therapy, tissue engineering, and tissue and organ regeneration comprising:
a. exposing fibroblastic cells to tissue specific signals to induce said cells to divide, engage in morphogenesis, differentiate into phenotypes unlike that of the dermal fibroblast, and or form tissues and or organs.
- 2. The method of claim 1, wherein said cells are derived from fetal or adult dermis and have the capacity to respond to said tissue specific signals.
- 3. The method of claim 1, wherein said tissues and or organs are unlike the dermis of the skin.
- 4. The method of claim 1, wherein said method further comprises creating large cultures of said cells of numbers between about 102 and 1015, which can be induced by said tissue specific signals to divide and differentiate along particular pathways of development for the purpose of forming tissues and or organs.
- 5. The method of claim 1, wherein said method further comprises delivering said cells to tissues of a recipient alone or in a delivery vehicle.
- 6. The method of claim 6, wherein said delivery comprises injecting said cells into said tissues of a recipient.
- 7. A tissue or organ-equivalent produced by the method of claim 1 comprising cells, signaling complexes and a scaffold.
- 8. The use of a tissue or organ-equivalent produced by the method of claim 1 in vitro or for implantation in vivo.
- 9. The method of claim 1, wherein said cells are cultivated for about three months in a medium comprising an extract derived from mES so that said cells express the stem cell marker CD34.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is related to co-pending U.S. Application No. 60/256,614, filed Dec. 18, 2000, No. 60/256,593, filed Dec. 18, 2000 and No. 60/251,125, filed Dec. 4, 2000 (from which it claims priority), and a U.S. application filed concurrently, entitled “Generation and Use of Signal-plexes to Develop Specific Cell Types, Tissues and/or Organs,” the entire contents of which are incorporated herein by reference.
Provisional Applications (3)
|
Number |
Date |
Country |
|
60256614 |
Dec 2000 |
US |
|
60256593 |
May 2001 |
US |
|
60251125 |
Dec 2000 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09901786 |
Jul 2001 |
US |
Child |
10430041 |
May 2003 |
US |