Patent Application
20060188453
The present invention relates to the use of substituted 2,4-bis(alkylamino)pyrimidines in the antimicrobial treatment of surfaces and to the preparation of such compounds.
The present invention relates to the use of 2,4-bis(alkylamino)pyrimidines of formula
wherein R1 is C1-C12alkyl or C6-C10aryl; R2 is hydrogen or C1-C12alkyl; or R1 and R2 together form a radical of formula
wherein R′ and R′″ are each independently of the other hydrogen, C1-C6alkyl or C1-C6alkoxy; R3 and R5 are each independently of the other hydrogen or C1-C8alkyl; R4 is C1-C20alkyl, unsubstituted phenyl, C6-C10aryl, preferred C7-C10aryl; C6-C10aryl—C-
C8alkyl, hydroxy—C1-C6alkyl, di—C1-C6alkylamino—C1-C6alkyl, mono—C1-C6alkylamino—C1-
C8alkyl, —(CH2)2-(O—(CH2)1—4-OH or —(CH2)2-(O—(CH2)2)1—4-NH2;
R6 is C1-C20alkyl, C8-C10aryl, C6-C10aryl—C1-C8alkyl, hydroxy—C1-Calkyl, di—C1-C8alkylamino—C1-Calkyl, mono—C1-C6alkylamino—C1-C6alkyl, —(CH2)2-(O—(CH2)2)1—4-OH or —(CH2)2-(O—(CH2)2)1—4-NH2; or R3 and R4 and/or R5 and R6 together form a pyrrolidine, piperidine, hexamethyleneimine or
morpholine ring; in the antimicrobial treatment of surfaces.
C1-C20Alkyl is straight-chain or branched alkyl, e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, tert-butyl, amyl, isoamyl or tert-amyl, hexyl, isohexyl, heptyl, octyl, isooctyl, nonyl, decyl, undecyl, dodecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl or eicosyl. C1-C12Alkyl is straight-chain or branched alkyl, e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, seo-butyl, tert-butyl, amyl, isoamyl or tert-amyl, hexyi, isohexyl, heptyl, octyl, isooctyl, nonyl, decyl, undecyl or dodecyl.
C1-C6Alkyl is straight-chain or branched alkyl, e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, tert-butyl, amyl, isoamyl or tert-amyl, isohexyl, hexyl, heptyl, octyl or isooctyl.
C1-C4Alkyl is straight-chain or branched alkyl, e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl or tert-butyl.
C1-C8Alkyl is straight-chain or branched alkyl, e.g. n-propyl, isopropyl, n-butyl, sec-butyl, tert-butyl, amyl, isoamyl or tert-amyl, isohexyl, hexyl, heptyl, octyl or isooctyl, especially hexyl.
C1-6Alkyl is straight-chain or branched alkyl, e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, tert-butyl, amyl, isoamyl or tert-amyl, hexyl or isohexyl.
C1-C6Alkoxy is a straight-chain or branched radical, e.g. methoxy, ethoxy, propoxy, butoxy, pentyloxy or hexyloxy.
C6-C10Aryl denotes naphthyl and especially phenyl. C6-C10Aryl radicals may be unsubstituted or may carry one or more, for example one, two, three or four, identical or different substituents, which may be in any desired position(s). Examples of such substituents are C1-C4alkyl, halogen, hydroxy, C1-C4alkoxy, trifluoromethyl, cyano, hydroxycarbonyl, C1-C4—alkoxycarbonyl, aminocarbonyl, amino, C1-C4alkylamino, di—C1-C4alkylamino and C1-C4-alkylcarbonylamino.
Special preference is given to compounds of formula (1) wherein R1 is C1-C8alkyl or phenyl; or to compounds of formula (1) wherein R2 is hydrogen or C3-C8alkyl; or to compounds of formula (1) wherein R3 and R5 are each independently of the other hydrogen or C1-C8alkyl; or to compounds of formula (1) wherein R4 is C1-C12alkyl, unsubstituted phenyl, C6-C10aryl—C1-C6alkyl, hydroxy—C2-C6alkyl, di—C1-C4-alkylamino—C1-C4alkyl,
mono—C1-C4alkylamino—C1-C4alkyl, —(CH2)2-(O—(CH2)2)1,2-OH or
—(CH2)2-(O—(CH2)2)1, 2-NH2; and R6 is C1-C12alkyl, C6-C10aryl, C6-C10aryl—C1C6alkyl, hydroxy—C2-C6alkyl, di—C1-C4alkylamino—C1-C4alkyl, mono—C1-C4alkylamino—C1-C4alkyl, —(CH2)2-(O—(CH2)2)1, 2-OH or —(CH2)2-(O—(CH2)2)1, 2-NH2; or to compounds of formula (1) wherein R3 and R4 and/or R6 and R6 together form a pyrrolidine, piperidine, hexamethyleneimine or morpholine ring.
Preference is given to the use according to the invention of compounds of formula
wherein R′ is hydrogen, C1-C3alkyl or C1-C3alkoxy; R″ is C1-C3alkyl or C1-C3alkoxy; R3 and R5 are each independently of the other hydrogen or C1-C8alkyl; and R4 and R6 are each independently of the other C1-C12alkyl, phenyl—C1-C3alkyl, hydroxy—C1-C6-alkyl
or di—C1-C6alkylamino—C1-C6alkyl, mono—C1-C6alkylamino—C1-C6alkyl, —(CH2)2-(O—(CH2)2)1—4OH or —(CH2)2-(O—(CH2)2)1—4-NH2; or R3 and R4 and/or R5 and R6 together form a pyrrolidine, piperidine, hexamethyleneimine or
morpholine ring.
Special preference is given to the use of compounds of formula (1) wherein R1 is C1-C8or phenyl; R2 is hydrogen or hexyl; or R1 and R2 together form a radical of formula (1a) wherein R′ is hydrogen, C1-C3alkyl or C1-C3alkoxy, and R″ is C1-C3alkyl or C1-C3alkoxy; R3 and R5 are each independently of the other hydrogen or C1-C8alkyl; R4 is C1-C12alkyl, unsubstituted phenyl, C6-C10aryl—C1-C6alkyl, hydroxy—C2-C6alkyl, di—C1-C4-alkylamino—C
1-C4alkyl, mono—C1-C4alkylamino—C1-C4alkyl, —(CH2)2-(O—(CH2)2)1,2-OH
or —(CH2)2-(O—(CH2)2)1,2-NH2; and R6 is C1-C12alkyl, C6-C10aryl, C6-C10aryl-C1-C6alkyl, hydroxy—C2C6alkyl, di—C1-C4alkyl—amino—C1-C4alkyl,
mono—C1-C4alkylamino—C1-C4alkyl, —(CH2)2-(O—(CH2)2)1,2-OH or —(CH2)2-(O—CH2)2)1,2-NH2; or R3 and R4 together, and R5 and R6 together, form a pyrrolidine, piperidine, hexamethyleneimine or morpholine ring.
There are used especially compounds of formula (1) wherein R3 and R5 , and R4 and R6 , have the same meanings.
Espedally preferred compounds are those of the following formulae
Examples of compounds used according to the invention are listed in Table 1:
The compounds used according to the invention are prepared according to methods known perse. The substituted 2,4-bis(alkylamino)pyrimidines are obtained by reacting the corresponding dichloropyrimidine compound (formula (1b)) with a primary or secondary amine—depending upon the meanings of the radicals R3 and R6 —in a suitable solvent, e.g. DMF, di—oxane, toluene, xylene, ethanol or butanol, and an auxiliary base, e.g. triethylamine, DIEA, sodium carbonate, potassium carbonate, etc., or using an excess of the amine compound, for a period of from 1 to 24 hours at 40-150° C. The reaction takes place according to the following Scheme (I):
or the compounds used according to the invention are prepared by condensing a guanidine compound with a suitable β-keto ester using an auxiliary base, e.g. sodium carbonate, potassium carbonate, sodium ethanolate, sodium methanolate or potassium tert-butanolate, in a suitable solvent, e.g. methanol, ethanol, butanol, tert-butanol, tetrahydrofuran, dimethylformamide, acetonitrile, toluene or xyiene, for a period of from 1 hour to 24 hours at a temperature of from 40 to 150° C. The resulting 2-alkylamino-4hydroxy-pyrimidine is then converted into the corresponding 2-alkylamino-4-chloro-pyrimidine compound according to customary methods by means of phosphorus oxychloride. The substituted 2,4-alkylamino-pyrimidines are obtained by reacting the 2-alkylamino—4-chloro-pyrlmidine compound with a primary or secondary amine (R4R5NH) in a suitable solvent, e.g. methanol, ethanol, butanol, tetrahydrofuran, dimethylformamide, dioxane, toluene or xylene, and an auxiliary base, e.g. triethylamine, DIEA, sodium carbonate, potassium carbonate or an excess of amine, for a period of from 1 to 24 hours at a temperature of from 40 to 150° C. The reaction takes place according to the following Scheme (II):
The 2,4-bis(alkylamino)pyrimidines used according to the invention exhibit a pronounced antimicrobial action, especially against pathogenic gram-positive and gram-negative bacteria and against bacteria of skin flora, and also against yeasts and moulds. They are therefore suitable especially in the disinfection, deodorisation and the general and antimicrobial treatment of the skin and mucosa and also of integumentary appendages (hair), more especially in the disinfection of the hands and of wounds.
They are therefore suitable as antimicrobial active ingredients and preservatives in personal care preparations, for example shampoos, bath additives, hair-care products, liquid and solid soaps (based on synthetic surfadtants and salts of saturated and/or unsaturated fatty adds), lotions and creams, deodorants, other aqueous or alcoholic solutions, e.g. cleansing solutions for the skin, moist deansing dloths, oils or powders.
The invention therefore relates also to a personal care preparation comprising at least one compound of formula (1) as well as cosmetically tolerable carriers or adjuvants. The personal care preparation according to the invention contains from 0.01 to 15 % by weight, preferably from 0.1 to 10 % by weight, based on the total weight of the composition, of a compound of formula (1) and cosmetically tolerable adjuvants.
Depending upon the form of the personal care preparation, it will comprise, in addition to the 2,4-bis(alkylamino)pyrimidine of formula (1), further constituents, for example sequestering agents, colourings, perfume oils, thickening or solidifying agents (consistency regulators), emollients, UV absorbers, skin-protective agents, antioxidants, additives that improve mechanical properties, such as dicarboxylic acids and/or Al, Zn, Ca and Mg salts of C14-C22 fatty acids, and optionally preservatives.
The personal care preparation according to the invention may be formulated as a water-in-oil or oil-in-water emulsion, as an alcoholic or alcohol-containing formulation, as a vesicular dispersion of an ionic or non-ionic amphiphilic lipid, as a gel, a solid stick or as an aerosol formulation.
As a water-in-oil or oil-in-water emulsion, the cosmetically tolerable adjuvant contains preferably from 5 to 50 % of an oily phase, from 5 to 20 % of an emulsifier and from 30 to 90 % water. The oily phase may contain any oil suitable for cosmetic formulations, e.g. one or more hydrocarbon oils, a wax, a natural oil, a silicone oil, a fatty acid ester or a fatty alcohol. Preferred mono- or poly-ols are ethanol, isopropanol, propylene glycol, hexylene glycol, glycerol and sorbitol.
Cosmetic formulations according to the invention may be used in a variety of fields. Especially the following preparations, for example, come into consideration:
skin-care preparations, e.g. skin-washing and cleansing preparations in the form of tablet-form or liquid soaps, synthetic detergents or washing pastes;
bath preparations, e.g. liquid (foam baths, milks, shower preparations) or solid bath preparations, e.g. bath cubes and bath salts;
skin-care preparations, e.g. skin emulsions, multi-emulsions or skin oils;
cosmetic personal care preparations, e.g. facial make-up in the form of day creams or powder creams, face powder (loose or pressed), rouge or cream make-up, eye-care preparations, e.g. eyeshadow preparations, mascara, eyeliner, eye creams or eye-fix creams; lip-care preparations, e.g. lipsticks, lip gloss, lip contour pencils, nail-care preparations, such as nail varnish, nail varnish removers, nail hardeners or cuticle removers;
intimate hygiene preparations, e.g. intimate washing lotions or intimate sprays;
foot-care preparations, e.g. foot baths, foot powders, foot creams or foot balsams, special deodorants and antiperspirants or callus-removing preparations;
light-protective preparations, such as sun milks, lotions, creams and oils, sun blocks or tropicals, pre-tanning preparations or after-sun preparations;
skin-tanning preparations, e.g. self-tanning creams;
depigmenting preparations, e.g. preparations for bleaching the skin or skin-lightening preparations;
insect-repellents, e.g. insect-repellent oils, lotions, sprays or sticks;
deodorants, such as deodorant sprays, pump-action sprays, deodorant gels, sticks or roll-ons;
antiperspirants, e.g. antiperspirant sticks, creams or roll-ons;
preparations for cleansing and caring for blemished skin, e.g. synthetic detergents (solid or liquid), peeling or scrub preparations or peeling masks;
hair-removal preparations in chemical form (depilation), e.g. hair-removing powders, liquid hair-removing preparations, cream- or paste-form hair-removing preparations, hair removing preparations in gel form or aerosol foams;
shaving preparations, e.g. shaving soap, foaming shaving creams, non-foaming shaving creams, foams and gels, preshave preparations for dry shaving, aftershaves or after-shave lotons;
fragrance preparations, e.g. fragrances (eau de Cologne, eau de toilette, eau de parfum, parfum de toilette, perfume), perfume oils or cream perfumes;
dental-care, denture-care and mouth-mare preparations, e.g. toothpastes, gel toothpastes, tooth powders, mouthwash concentrates, anti-plaque mouthwashes, denture deaners or denture fixatives;
cosmetic hair-treatment preparations, e.g. hair-washing preparations in the form of shampoos and conditioners, hair-care preparations, e.g. pretreatment preparations, hair tonics, styling creams, styling gels, pomades, hair rinses, treatment packs, intensive hair treatments, hair-structuring preparations, e.g. hair-waving preparations for permanent waves (hot wave, mild wave, cold wave), hair-straightening preparations, liquid hair-setting preparations, hair foams, hairsprays, bleaching preparations, e.g. hydrogen peroxide solutions, lightening shampoos, bleaching creams, bleaching powders, bleaching pastes or oils, temporary, semi-permanent or permanent hair colourants, preparations containing seif-oxidising dyes, or natural hair colourants, such as henna or camomile.
An antimicrobial soap has, for example, the following composition: 0.01 to 5% by weight of a compound of formula (1) 0.3 to 1% by weight titanium dioxide 1 to 10% by weight stearic acid ad 100% soap base, e.g. the sodium salts of tallow fatty acid and coconut fatty acid or glycerol.
A shampoo has, for example, the following composition: 0.01 to 5% by weight of a compound of formula (1) 12.0% by weight sodium laureth-2-sulfate 4.0% by weight cocamidopropyl betaine 3.0% by weight NaCI and water ad 100%.
A deodorant has, for example, the following composition: 0.01 to 5% by weight of a compound of formula (1) 60% by weight ethanol 0.3% by weight perfume oil and water ad 100%.
The invention relates also to an oral composition containing from 0.01 to 15% by weight, based on the total weight of the composition, of a compound of formula (1) and orally tolerable adjuvants.
Example of an oral composition: 10% by weight sorbitol 10% by weight glycerol 15% by weight ethanol 15% by weight propylene glycol 0.5% by weight sodium lauryl sulfate 0.25% by weight sodium methyloocyl taurate 0.25% by weight polyoxypropylene/polyoxyethylene block copolymer 0.10% by weight peppermint flavouring 0.1 to 0.5% by weight of a compound of formula (1) and 48.6% by weight water.
The oral composition according to the invention may be, for example, in the form of a gel, a paste, a cream or an aqueous preparation (mouthwash).
The oral composition according to the invention may also comprise compounds that release fluoride ions which are effective against the formation of caries, for example inorganic fluoride salts, e.g. sodium, potassium, ammonium or calcium fluoride, or organic fluoride salts, e.g. amine fluorides, which are known under the trade name Olafluor.
The 2,4-bis(alkylamino)pyrimidines of formula (1) used according to the invention are also suitable for the treatment, especially preservation, of textile fibre materials. Such materials are undyed and dyed or printed fibre materials, e.g. of silk, wool, polyamide or polyurethanes, and especially cellulosic fibre materials of all kinds. Such fibre materials are, for example, natural cellulose fibres, such as cotton, linen, jute and hemp, as well as cellulose and regenerated cellulose. Preferred suitable textile fibre materials are made of cotton.
The 2,4-bis(alkylamino)pyrimidines according to the invention are also suitable for the treatment of plastics, especially for imparting antimicrobial properties to or preserving plastics, e.g. polyethylene, polypropylene, polyurethane, polyester, polyamide, polycarbonate, latex etc.. Fields of use therefor are, for example, floor coverings, plastics coatings, plastics container and packaging materials; kitchen and bathroom utensils (e.g. brushes, shower curtains; sponges, bathmats), latex, filter materials (air and water filters), plastics articles used in the field of medicine, e.g. dressing materials, syringes, catheters etc., so-called “medical devices”, gloves and mattresses.
Paper, for example papers used for hygiene purposes, may also be provided with anti-microbial properties using the 2,4-bis(alkylamino)pyrimidines of formula (1) according to the invention.
It is also possible for nonwovens, e.g. nappies/diapers, sanitary towels, panty liners, and doths for hygiene and household uses, to be provided with antimicrobial properties in accordance with the invention.
The 2,4-bis(alkylamino)pyrimidines of formula (1) are also used in washing and deaning formulations, e.g. in liquid and powder washing agents or softeners.
The 2,4-bis(alkylamino)pyrimidines of formula (1) may especially also be used in household and all-purpose cleaners for deaning and disinfecting hard surfaces.
A cleaning preparation has, for example, the following composition: 0.01 to 5% by weight of a compound of formula (1) 3.0% by weight octyl alcohol 4EO 1.3% by weight fatty alcohol C8-C10polyglucoside 3.0% by weight isopropanol ad 100% by weight water.
In addition to preserving cosmetic and household products, the preservation of technical products, the provision of technical products with antimicrobial properties and use as a biocide in technical processes are also possible, for example in paper treatment, especially in paper treatment liquors, in printing ink thickeners consisting of starch or of cellulose derivatives, in surface-coating compositions and in paints.
The 2,4-bis(alkylamino)pyrimidines of formula (1) are also suitable for the antimicrobial treatment of wood and for the antimicrobial treatment of leather, the antimicrobial preservation of leather and the provision of leather with antimicrobial properties.
The compounds according to the invention are also suitable for the protection of cosmetic products and household products from microbial spoilage.
In addition to their generally antimicrobial action, the 2,4bis(alkylamino)pyrImidlnes of formula (1) according to the invention are moreover capable of penetrating bloflims on living and non-living surfaces, of preventing the adhesion of bacteria to surfaces and any further build-up of the bloflim, of detaching such biofilm and/or inhibiting the further growth of the biofilm-forming micro-ogranisms in the biological matrix, or of killing such micro-organisms.
Biofilms are understood, very generally, to be aggregations of living and dead micro-organisms, especially bacteria, that adhere to living and non-living surfaces, together with their metabolites in the form of extracellular polymeric substances (EPS matrix), e.g. polysaccharides. The activity of antimicrobial substances that normally exhibit a pronounced growth-inhiblbng or lethal action with respect to planktonic cells may be greatly reduced with respect to microorganisms that are organised in bloflims, for example because of inadequate penetration of the active substance into the biological matrix.
In the present invention, this relates, very especially, to bioflims on human tooth surfaces and oral mucosa, which play a crucial role in the onset of degenerative diseases in the oral cavity, e.g. caries or periodontitis, as a result of the biofilm-forming micro-organisms or their metabolites.
The following Examples illustrate, but do not limit, the present invention.
8.15 g of 2,4-dichloro-6-methyl-pyrimidine (50 mmol) are heated with 19.39 g of octylamine (150 mmol) and 20.73 g of potassium carbonate (150 mmol) in 20 ml of dioxane for 16 hours at 1009°C. After cooling, the product is taken up in 300 ml of ethyl acetate and washed with 0.5 mol/litre of sodium hydroxide solution and saturated sodium chloride solution. The product is concentrated in vacuo and then octylamine is distilled off for 2 hours at 140° C. under a rotary slide valve vacuum. 12.95 g of N,N′—bis(2,4-dioctylamino)-6-methylpyrimidine (37.15 mmol, 74.3% of theory) are obtained. The end product is analysed by NMR, HPLC—MS, GC and HPLC. GC: 98% area M+1=349 NMR (1H in DMSO): 0.85, t, 6H; 1.25, m, 20H; 1.5, m, 4H; 2, s, 3H; 4.2, m, 4H; 5.5, s, 1 H; 6.2, s, 1H; 6.6; s, 1H.
8.15 g of 2,4-dichloro-methyl-pyrimidine (50 mmol) are heated with 16 g of benzylamine (150 mmol) and 20.73 g of potassium carbonate (150 mmol) in 20 ml of dioxane for 16 hours at 100° C. After cooling, the product is taken up in 300 ml of ethyl acetate and washed with 0.5 mol/litre of sodium hydroxide solution and saturated sodium chloride solution. The product is concentrated in vacuo and then benzylamine is distilled off for 2 hours at from 105 to 120° C. under a rotary slide valve vacuum, and the product is recrystallised from isopropanol. N,N′—Bis(2,4-dibenzylamino)-6-methylpyrimidine is obtained in a yield of 76%. Purity: GC 100% NMR (1H in DMSO, ppm): 2, s, 3H; 4.45, m, 4H; 5.6, s, 1H; 6.95, s, 1H; 7.25, m, 11H.
8.15 9 of 2,4-dichloro6-methyl-pyrimidine (50 mmol) are heated with 18.17 9 of phenyl-ethylamine (150 mmol) and 20.73 g of potassium carbonate (150 mmol) in 20 ml of dioxane for 16 hours at 100° C. After cooling, the product is taken up in 300 ml of ethyl acetate and washed with 0.5 mol/litre of sodium hydroxide solution and saturated sodium chloride solution. The product is concentrated in vacuo and then phenylethylamine is distilled off for 2 hours at 150° C. under a rotary slide valve vacuum and the product is recrystallised from isopropanol. N,N′-Bis(2,4-diphenylethylamino)-6-methylpyrlmidine is obtained in a yield of 98%. Purity: GC 100% HPLC 98% NMR (I1H in DMSO, ppm): 2, s, 3H; 2.9, t, 4H; 3.45, m, 4H; 5.6, 8, 1H; 6.45, 8, IH; 6.8, 8, 1-H; 7.25, m, 10OH.
7 g (20 mmol) of phenylguanidine carbonate are reacted in 5 ml of absolute ethanol with 27.2 9 (80 mmol) of a 20% sodium ethanolate solution. 11.5 9 of ethylbenzoyl acetate (59.8 mmol) are added dropwise thereto in the course of 15 minutes at 75° C. The reaction mass is then stirred for 15 hours at 70° C. and, after cooling, is extracted with 50 ml of di—chloromethane and washed three times with 40 ml of water/3 ml of acetic acid. The organic phase is dried over sodium sulfate and concentrated by evaporation. 5.86 9 (55.7% of theory) of 4-hydroxy-2-phenylamino-6-phenylpyrimidine are obtained.
NMR (1H in DMSO, ppm): 6.45, s,1H; 7.05, t, 1H; 7.4, t, 2H; 7.5, m, 3H; 7.75, d, 2H; 8, m, 2H;9,s, 11H; 11.05,s, 1H.
2 g (7.6 mmol) are reacted in 10 ml of toluene with 3.5 g of phosphorus oxychloride. The reaction mass is heated to 80° C and 1.53 g of triethylamine (15.1 mmol) are added dropwise thereto in the course of 20 minutes. After a reaction time of 2 hours at 800C, the mass is cooled in an ice bath and 28 ml of 4M sodium hydroxide solution are added dropwise thereto. The aqueous phase is extracted three times with ethyl acetate. After concentration of the organic phase by evaporation, 2.12 9 (99.1% of theory) of 4- chloro-2-phenyl-amino-6-phenylpyrimidine are obtained. NMR (1H in DMSO, ppm): 7, t, 1IH; 7.3, t, 2H; 7.55, m, 4H; 7.8, d, 2H; 8.2, m, 2H; 10.05, s, 11H.
The reactions are carried out in parallel robotically. 56.3 mg of 4-chlorm-2-phenylamino-6-phenylpyrimidine (0.2 mmol) are dissolved in 0.5 ml of dioxane. 38.7 mg of diisopropylamine (0.3 mmol) and 3 mmol of amine are added thereto and the reaction mixture is heated at 85° C. for 21 hours. After cooling, the mass is extracted with 2 ml of dichioromethane and washed three times with 1.125 ml of acetic add (13% in water) and 1.2 ml of sodium hydroxide solution. The organic phases are dried and lyophillsed.
The compounds (PY10) - (PY29) (see Table 1) are prepared according to this method. They were analysed by LC-MS.
34.65 g of octylguanidine acetate A (0.15 mol) are reacted in 30 ml of ethanol with 102 g of 20% sodium ethanolate solution in ethanol (0.3 mol). The reaction mixture is heated to 75° C., and 26.15 g of methyl acetate B (0.22 mol) are added thereto in the course of one hour, and the mixture is stirred for 12 hours. After cooling, the reaction mass is diluted with dichloro-methane and washed three times with water/acetic add and twice with sodium hydroxide so- luton. The combined alkaline aqueous phases are adjusted to pH 6 with acetic acid and ex- tracted with dichloromethane, dried over sodium sulfate and concentrated by evaporation. 30.74 9 of compound C (87% of theory) are obtained. Purity in HPLC: 99% NMR (in CD2CI2 in ppm): 0.9, t, 3H; 1.3, m, 1 OH; 1.6, qt, 2H; 2.2, s, 3H; 3.35, m, 2H; 5.6, s, 1H; 6.7, s, IH.
18.96 g (0.08 mol) of the compound of formula C are reacted in 60 ml of toluene with 36.85 g of phosphorus oxychloride. The reaction mass is heated to 80° C. After a reaction time of 2 hours at 80° C., the mass is cooled in an Ice bath and 4M sodium hydroxide solution is added dropwise thereto. The aqueous phase is extracted three times with toluene. After concentration of the organic phase by evaporation, 20.04 g (98% of theory) of the compound of formula D are obtained. Purity in GC: 100% NMR (in CD2CI2 in ppm): 0.8, t, 3H; 1.3, m, 10H; 1.55, qt, 2H; 2.2, s, 3H; 3.3, q, 2H; 5.25, a, 1H; 6.35, s, 1H.
12.96 g of compound D (0.048 mol) are mixed with 6.19 g of di-isopropylamine (0.048 mol) in 60 ml of dioxane and heated at reflux. 4.9 g of pyrrolidine (0.057 mol) are added dropwise thereto in the course of 25 minutes and the reaction mixture is stirred at reflux for 29 hours. After cooling, the reaction mass is diluted with dichloromethane and washed three times with water/acetic acid and twice with sodium hydroxide solution. The organic phase is dried over sodium sulfate and concentrated by evaporation. 12.73 g of compound PY44 (91.4% of theory) are obtained. Purity in GC: 100% NMR (CD2CI2 in ppm): 0.8, t, 3H; 1.2, m, 10H; 1.45, qt, 2H; 1.85, m, 4H; 2, 5, 3H; 3.2—3.3, m (2 signals), 6H; 4.7, s, 1 H; 5.45 s, 1H.
18.48 g of octylguanidine acetate A (0.08 mol) are reacted in 15 ml of ethanol with 54.4 g of 20% sodium ethanolate solution in ethanol (0.1 6 mol). The reaction mixture is then heated to 75° C and 24 9 of methyl 2-hexylacetoacetate E (0.12 mol) are added thereto in the course of 30 minutes, and the mixture is sUrred overnight. After cooling, the reaction mass is diluted with dichloromethane and washed twice with water/acetic acid. The organic phase is dried over sodium sulfate and concentrated by evaporation. The crude product is recrystallised from acetone. 14.86 g of compound F (57.9% of theory) are obtained. LC-MS: a compound having M =321.
13.16 g (0.041 mol) of a compound of formula F are reacted in 40 ml of toluene with 18.89 g of phosphorus oxychloride. The reaction mass is heated to 80° C. After a reaction time of 2 hours at 80° C., the mass is cooled in an ice bath and 4M sodium hydroxide solution is added dropwise thereto. The aqueous phase is extracted three times with toluene.
After concentration of the organic phase by evaporation, 13.65 g (98% of theory) of the compound of formula G are obtained. Purity in GC: 100% NMR (CD2CI2 in ppm): 0.9, m, 6H; 1.3, m, 18H; 1.5, m, 2H; 1.6, m, 2H; 2.4, 8, 3H; 2.6, t, 2H; 3.4, q, 2H; 5.6, s, 1H.
11.88 g of compound G (0.035 mol) are stirred with 51.87 g of 1,8-diamino—3, 6-dioxaoctane (0.35 mol) and 6.77 g of diisopropylamine (0.0525 mol) at 110° C. for 23 hours. After cooling, the reaction mass is diluted with dichloromethane and washed three times with water and twice with waterlacetic acid. The combined aqueous phases are adjusted to pH 9 with sodium hydroxide solution and extracted with dichloromethane, dried over sodium sulfate and concentrated by evaporation. 14.45 g of compound PY55 (64.6% of theory) are obtained. Purity in GC: 100% NMR (CD2CI2 in ppm): 0.9, m, 6H; 1.3, m, 20H; 1.55, m, 2H; 2.15, s, 3H; 2.3, t, 2H; 2.4, s, 2H; 2.8, t, 2H; 3.3, q, 2H; 3.45, t, 2H; 3.6, m, 8H; 5.1, s, 1H; 5.7, s, 1H.
Nutrient medium: Casein/soybean flour peptone bouillon for the preparation of the precultures of the test bacteria and yeast.
The test substances are predissolved in dimethyl sulfoxide (DMSO) and tested in a serial dilution of 1:2.
Bacteria and yeast are cultured overnight in CASO bouillon.
All test organism suspensions are adjusted to an organism count of 1-5×108 CFU/ml with 0.85% sodium chloride solution. The test substances are prepipetted into microtitre plates in an amount of 8 μl per well.
The previously adjusted organism suspensions are diluted 1:100 in CASO bouillon and added to the test substances in an amount of 192 μl per well.
The test batches are incubated for 48 hours at 37° C. After incubation, the growth is deternined by reference to the turbidity of the test batches (optical density) at 620 nm in a microplate reader.
The minimum inhibiting concentration (MIC value) is the concentration of substance at which (compared with the growth control) an appreciable inhibition of the growth (≲20 % growth) of the test organisms is ascertained.
Three microtitre plates are used for each test organism and substance concentration.
Table 2 shows the microbiological test results:
Medium: Caseinisoybean flour peptone agar ( Merck) *Sabouraud 4% glucose agar (Merck)
Dilution medium: sterile 0.85% NaCI solution
Test organisms: Staphylococcus aureus ATCC 6853 and 9144
Incubation: 24 hours at 37° C.
Test solution: 1% stock solutions of all the test substances in a suitable solvent are
Test principle:
0.3 ml of the dilution stage in question is mixed with 15 ml of still-liquid nutrient medium. When the nutrient substrate has solidified, 10 μl portions of a suitable organism dilution of the test strains in 0.85% NaCI solution are spotted onto the agar medium:
Staphylococcus aureus ATCC 6538
Staphylococcus aureus ATCC 9144
Staphylococcus epidermidis ATCC 12228
C. xerosis ATCC 373**
C. minutissimum ATCC 23348**
Propionibacterium acnes ATCC 6919***
Escherichia coli NCTC 8196
Escherichia coli ATCC 10536
Proteus vulgaris ATCC 6896
Klebsiella pneumoniae ATCC 4352
Salmonella choleraesuis ATCC 9184
Pseudomonas aeruginosa ATCC 15442
Candida albicans ATCC 10231
Aspergillus niger ATCC 6275
*very slow growth, no growth in the next dilution stage
**3 days incubation,
***3 days incubation under anaerobic conditions
Medium: thioglycolate bouillon with hemin and menadione
Dilution medium: the appropriate amount of the substances was pipetted directly into
Test organisms: Actinobacillus actinomycetemoomitans ATCC 43718
Incubation: 7-10 days at 37° C anaerobic, or 24 hours aerobic with 10% CO2 for
Test solution: Stock solutions of all the test substances in ethanol at 1500 ppm (w/w)
Test princlple:
Bacteria are removed from blood agar plates using cotton wool buds and a suitable optical density (McFarland 0.5) is adjusted in an appropriate medium; that solution is used undiluted for F. nucleatum and P. nigrescens, and in a dilution of 1:20 for the other strains. 0.1 ml of bacterial culture is added per 2 ml of active ingredient solution and incubation is carried out as described above.
| Number | Date | Country | Kind |
|---|---|---|---|
| 03102296.5 | Jul 2003 | EP | regional |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP04/51516 | 7/16/2004 | WO | 1/23/2006 |
