Claims
- 1. In a solid phase extraction process for the purification and separation of protein from a sample the improvement comprising employing as the solid phase a sulfonic derivative of covalently bound, non-crosslinked polyethyleneimine bonded phase silica of the formula ##STR5## wherein
- is the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --CH.sub.2).sub.x H or --CH.sub.2).sub.m COOH where x is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --CH.sub.2).sub.x H then R.sup.1 is --CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --CH.sub.2).sub.y H where y is an integer of zero or 1.
- 2. A process according to claim 1 wherein the silica backbone of the bonded phase is selected from particulate silica gel having an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 1000 .ANG. or particulate controlled pore glass having an average particle diameter of from about 37 to about 177 microns and an average pore size of from about 40 to about 1000 .ANG..
- 3. A process according to claim 2 wherein the silica backbone of the bonded phase is particulate silica gel having an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 1000 .ANG..
- 4. A process according to claim 3 wherein q=zero, R is hydrogen, R.sup.1 is --CH.sub.2).sub.p COOH and p is zero.
- 5. A process according to claim 3 wherein q is zero, R is --CH.sub.2).sub.x H, x is 1, R.sup.1 is --CH.sub.2).sub.p COOH and p is zero.
- 6. A process according to claim 3 wherein q is 1, R is hydrogen, R.sup.1 is --CH.sub.2).sub.p COOH and p is zero.
- 7. A process according to claim 3 wherein q is zero, R is --CH.sub.2).sub.m COOH, m is 1 and R.sup.1 is hydrogen.
- 8. A process according to claim 2 wherein the particulate silica gel has an average particle derivative of from about 5 to about 40 microns and an average pore size of from about 50 to about 330 .ANG., an n is an integer such that the polyethyleneiminopropyl silane moiety has an average molecular weight of from about 400 to about 600.
- 9. A process according to claim 2 wherein the particulate silica gel has an average particle derivative of from about 40 to about 62 microns and an average pore size of from about 210 to about 520 .ANG., an n is an integer such that the polyethyleneiminopropyl silane moiety has an average molecular weight of from about 1000.
- 10. In a solid extraction process for the purification and separation of proteins having an isoelectric point of below about 5 the improvement comprising employing as the solid phase a sulfonic derivative of covalently bound, non-crosslinked polyethyleneimine bonded phase silica of the formula ##STR6## wherein
- is the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --CH.sub.2).sub.x H or --CH.sub.2).sub.m COOH where x is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --CH.sub.2).sub.x H then R.sup.1 is --CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R --CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --CH.sub.2).sub.y H where y is an integer of zero or 1.
- 11. A solid extraction process according to claim 10 wherein the silica backbone of the bonded phase is selected from particulate silica gel having an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 1000 .ANG. or particulate controlled pore glass having an average particle diameter of from about 37 to about 177 microns and an average pore size of from about 40 to about 1000 .ANG..
- 12. In a solid extraction process according to claim 11 wherein the silica backbone of the bonded phase is particulate silica gel having an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 1000 .ANG..
- 13. In a solid extraction process according to claim 12 wherein q=zero, R is hydrogen, R.sup.1 is --CH.sub.2).sub.p COOH and p is zero.
- 14. A solid phase extraction process according to claim 13 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
- 15. In a solid extraction process according to claim 12 wherein q is zero, R is --CH.sub.2).sub.x H, x is 1, R.sup.1 is --CH.sub.2).sub.p COOH and p is zero.
- 16. A solid phase extraction process according to claim 15 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
- 17. In a solid extraction process according to claim 12 wherein q is 1, R is hydrogen, R.sup.1 is --CH.sub.2).sub.p COOH and p is zero.
- 18. A solid phase extraction process according to claim 17 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
- 19. In a solid extraction process according to claim 12 wherein q is zero, R is --CH.sub.2).sub.m COOH, m is 1 and R.sup.1 is hydrogen.
- 20. A solid phase extraction process according to claim 19 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
- 21. A solid phase extraction process according to claim 12 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
- 22. In a solid extraction process according to claim 11 wherein the particulate silica gel has an average particle derivative of from about 5 to about 40 microns and an average pore size of from about 50 to about 330 .ANG., an n is an integer such that the polyethyleneiminopropyl silane moiety has an average molecular weight of from about 400 to about 600.
- 23. A solid phase extraction process according to claim 22 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
- 24. In a solid extraction process according to claim 11 wherein the particulate silica gel has an average particle derivative of from about 40 to about 62 microns and an average pore size of from about 210 to about 520 .ANG., an n is an integer such that the polyethyleneiminopropyl silane moiety has an average molecular weight of from about 1000.
- 25. A solid phase extraction process according to claim 24 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
- 26. A solid phase extraction process according to claim 11 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
- 27. A solid phase extraction process according to claim 10 for the purification and separation of a protein selected from ovalbumin and bovine serum albumin.
RELATED APPLICATION
This Application is a continuation-in-part of copending Application Ser. No. 837,051, filed Mar. 6, 1986, now U.S. Pat. No. 4,661,248.
US Referenced Citations (6)
Foreign Referenced Citations (1)
Number |
Date |
Country |
5380291 |
Jul 1978 |
JPX |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
837051 |
Mar 1986 |
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