Patent Application
20050148557
The present invention relates to the use of a Vitamin D receptor activator (VDRA) or a Vitamin D analog, preferably paricalcitol, to treat, prevent and delay progression of kidney disease.
The prevalence of end-stage renal disease (ESRD) is increasing at an alarming rate. In 2000, end stage kidney disease developed in over 90,000 people in the United States. The current population of patients on dialysis therapy or needing transplantation is 380,000 and projected to be 651,000 patients in 2010. Care for patients with ESRD already consumes more than $18 billion per year in the U.S, a substantial burden for the health care system. New data released in 2003 reported that 19.5 million U.S. adults have chronic kidney disease (CKD), and 13.6 million had Stage 2-5 CKD, as defined by the National Kidney Foundation Kidney Disease Outcomes Quality Initiative (NKF K/DOQI). Adverse outcomes of chronic kidney disease can often be prevented or delayed through early detection and treatment.
The pathogenesis for progression of renal fibrosis occurs through two mechanisms, which are additive: glomerulosclerosis and tubulointerstitial fibrosis (TIF).
Insults to the glomerula from hemodynamic, immune or metabolic systems can injure endothelial, epithelial or mesangial cells in the kidney through the body's inflammatory and hemodynamic adaptive processes. As a result, mesangial cells proliferate, leading to glomerular fibrosis (glomerulosclerosis). This fibrotic mechanism causes proteinuria, increases cytokines and TGF-β, leading to nephron loss. Glomerulosclerosis decreases the glomerular filtration rate (GFR). In humans, as GFR falls, kidney function and mass decline, even after the original disease becomes inactive. Surviving nephrons attempt to compensate by adapting their structure and function to meet excretory demands, leading to glomerular hyperfiltration and hypertrophy. Glomerular capillary hypertension is often maintained by angiotensin dependent mechanisms. Angiotensin II (AII) has emerged as a central mediator of the glomerular hemodynamic changes associated with progressive renal injury. This glomeruli hemodynamic adaptation further damages glomeruli and exacerbates glomerulosclerosis and nephron loss.
Angiotensin-converting enzyme inhibitors (ACEIs) and/or angiotensin receptor blockers (ARBs) plus/minus aldosterone blockade are the current regimen to treat hypertension (HTN), congestive heart failure (CHF), diabetic nephropathy (DN) and delay the progression of chronic kidney disease (CKD). Their effects on CKD are independent to their effects on controlling BP and treating HTN. In most cases, these therapies slowed the progression of CKD but did not arrest the decline to ESRD.
An important limitation of long-term use of ACEI and/or ARB is that these may lead to renin accumulation and the increase in downstream proteins, which may lead to an escape of ACE inhibition pathway with subsequent increase in AII and aldosterone generation. Aldosterone blockage in addition to ACEI and/or ARB to avoid aldosterone escape has additional benefit in the prevention of organ damage, but the renin level is still elevated in some patients. Additionally, incomplete arrest is explained by the fact that ACEI and ARB mainly target glomerular pathology and have weak effects on TIF.
TIF severity recently has been shown to correlate more highly with renal function than with glomerulosclerosis, resulting from a metabolic, immune or hemodynamic insult to the kidney. Renal TIF involves the following key and newly understood steps: 1) loss of adhesion of tubular epithelial cells and loss of cellular integrity by down regulation of E-cadherin; 2) transdifferentiation of tubular epithelial cells through de novo alpha-smooth muscle actin expression and actin reorganization of those epithelial cells that have lost adhesion; 3) disruption of the tubular basement membrane by increased matrix metalloproteinase (MMP) activity; and 4) transdifferentiated tubular cells that migrate and invade the interstitium, become myofibroblasts and cause fibrosis. Interruption of an early step in the pathway that leads to TIF could be an advantageous treatment. However, the market lacks such a medication.
It has been shown that the decreased serum vitamin D level correlates with decreased GFR and renal fibrosis. E. Ishimura, et al., Serum Levels of 1, 25 Dihydroxyvitamin D, 24,25 dihydroxyvitamin D, 25-hydroxyvitamin D in nondialyzed patients with chronic renal failure, Kidney International, Vol. 55 (1999) p. 1019-1027. However, the role of Vitamin D, if any, in the disease process itself has not been well understood before now. Researchers have studied whether VDRAs have a protective effect on the kidneys. Five recent studies have confirmed VDRAs can prevent glomerular injury and glomerulosclerosis. The studies claimed that vitamin D inhibits mesangium proliferation and inflammation, thereby ameliorating glomerular fibrosis.
Beyond effects on inflammation and proliferation, we believe that VDRAs can delay progression of chronic kidney disease by inhibiting renin secretion, which would prevent or reduce the ACE escape and the subsequent mesangial proliferation and glomerulosclerosis, and, more importantly, by preventing tubular interstitial fibrosis by blocking tubular epithelial to myofibroblast transdifferentiation.
Recent literature discloses that endogenous VDRA (calcitriol) could down regulate renin gene expression. See for example, Y. Li, et al., 1,25-Dihydroxyvitamin D3 is a negative endocrine regulator of the renin-angiotensin system, J. Clin. Invest., July, 2002 (incorporated herein by reference). According to the present invention, down regulation of renin by VDRAs can prevent or can reduce ACE escape which will have an additive or synergistic effect to therapy with ACEI, ARB and/or aldosterone blockers in preventing glomerulosclerosis.
Besides targeting pathogenesis of glomerulosclerosis through RAAS, VDRAs could increase E-cadherin expression to keep the integrity of the tubular cells, could decrease alpha-smooth muscle actin expression to prevent epithelial to myofibroblast transdifferentiation and could decrease MMP activity to prevent tubular basement disruption and cell migration. The summary of these effects would result in blocking the tubular epithelial myofibroblast transdifferentiation and preventing TIF.
As shown in
A multi-drug approach according to the present invention which blocks both pathways for renal disease progression would be advantageous. The present invention is therefore directed to advantageous combinations of a VDRA or Vitamin D analog with an ACE inhibitor and/or an angiotensin receptor blocker and/or aldosterone inhibitor.
The present invention is directed to methods for preventing, treating and delaying progression of kidney disease, including chronic kidney disease and pharmaceutical compositions useful therefor. According to one embodiment, the present invention relates to VDRA/Vitamin D analog-containing compositions for preventing, treating and delaying progression of kidney disease.
According to some aspects of the present invention, Vitamin D receptor activator (VDRA) compounds can be used. VDRAs include paricalcitol, calcitriol, 22-oxa-1-alpha,25-dihydroxyvitaminD2, MC-903 (calcipotriol), 16-ene-23-yne-1 alpha, 25-dihydroxyvitamin D3, and 24-difluoro-26,27-dimethyl-16-ene-1 alpha, 25-dihydroxyvitamin D3 (described in greater detail by DeLuca, et al., in PNAS, 2004, vol. 101, No. 18, p. 6900-6904, incorporated herein by reference), compounds listed in Table 1 of Physiol. Rev. October 1998, Vol. 78, No. 4, p 1193-1231, incorporated herein by reference in its entirety, and the so-called Gemini compounds (described in greater detail by Maehr, et al. in J. Steroid Biochem. Mole. Biol. 89-90, 2004, 35-38, incorporated herein by reference), EB-1089 (a LEO Pharmaceuticals compound), and ED-71 (a Roche compound). Paricalcitol is especially preferred since it is a selective VDRA. Paricalcitol is commercially available from Abbott Laboratories (North Chicago, Ill., under the tradename ZEMPLAR).
According to other aspects of the present invention, the Vitamin D analog can be doxercalciferol or alfacalcidol.
Especially preferred compositions of the present invention include a VDRA/Vitamin D analog and one or more of the following agents: an angiotensin converting enzyme inhibitor (ACEI) or an angiotensin II receptor 1 (ARB) blocker or an aldosterone blocker.
According to other aspects of the invention, pharmaceutical compositions can be administered through a sustained (or continuous) delivery system. The present invention also contemplates other modes of administration, including but not limited to oral, injectable and transdermal.
The present invention is generally directed to compositions containing a VDRA/vitamin D analog to treat or prevent kidney disease, including chronic kidney disease. The present invention also relates to methods of treating kidney disease by administering to a patient a pharmaceutical composition containing a therapeutically effective amount of a VDRA/Vitamin D analog.
Treatment of patients with kidney disease by administration of a therapeutically effective amount of a VDRA/Vitamin D analog-containing composition according to the invention can be advantageous because the VDRA/Vitamin D analog can act at any one or all of the following points in the renal biochemical pathway:
In contrast, conventional treatments based on administration of an ACEI (i.e., without a VDRA/Vitamin D analog), for example, only reduce angiotensin (II), but lack these other effects. Administration of ACEI may not be an attractive long term treatment due to adverse consequences.
According to some aspects of the present invention, the inventive compositions contain a VDRA/Vitamin D analog and at least one of the following agents: an ACE inhibitor, an angiotensin (II) receptor blocker (ARB) and aldosterone blocker in therapeutically effective amounts to inhibit renin production or inhibit activation of the renin-angiotensin-aldosterone system. Preferred compositions contain paricalcitol with at least one of these other agents. Such combinations can avoid ACE inhibition escape and aldosterone escape with subsequent increase in angiotensin (II) and aldosterone generation.
Suitable ACE inhibitors, ARB and aldosterone blockers are commercially available. Suitable ACE inhibitors include, but are not limited to: captopril (commercially available under the tradename CAPOTEN from Mylan), enalapril (commercially available under the tradename VASOTEC from Merck), fosinapril (commercially available under the tradename MONOPRIL from Bristol Myers Squibb), benzapril (commercially available under the tradename LOTENSIN from Novartis Pharmaceuticals), moexipril (commercially available under the tradename UNIVASC from Schwarz Pharma), perindopril (commercially available under the tradename ACEON from Solvay), quinapril (commercially available under the tradename ACCUPRIL from Parke-Davis), ramipril (commercially available under the tradename ALTACE from Monarch), trandolapril (commercially available under the tradename MAVIK from Abbott Laboratories of North Chicago, Ill.), lisinopril (commercially available under the tradenames PRINIVIL from and ZESTRIL from Astra Zeneca). Suitable angiotensin receptor blocking agents include, but are not limited to: losartan (commercially available as COZAAR from Merck), irbesartan (commercially available as AVAPRO from Bristol Myers Squibb and Sanofi), candesartan (commercially available as ATACAND from Astra Zeneca), eprosartan (commercially available as TEVETEN from Biovail Corporation of Canada), telmisartan (commercially available as MICARDIS from Boehringer Ingelheim) and valsartan (commercially available as DIOVAN from Novartis).
Suitable aldosterone blockers include, but are not limited to: eplerenone (commercially available under the tradename INSPRA from Pharmacia), spironolactone (commercially available under the tradenames Aldactone, Adultmin, Aldopur, Aldospirone, Almatol, Berlactone, Diatensec, Diram, Esekon, Hypazon, Idrolattone, Merabis, Novospiroton, Osiren, Osyrol, Pirolacton, Resacton, Sincomen, Spiractin, Spiroctan, Spirolacton, Spirolang, Spironex, Spirotone, Tevaspirone, Verospiron, Xenalon Lactabs, Youlactone).
Additional components, e.g., physiologically acceptable carriers, solvents, binders, antioxidants, colorants, substrates can be used as necessary or desired.
Preferred treatment or preventive regimens for patients with kidney disease according to the present invention would administer therapeutically effective VDRA/Vitamin D analog-containing compositions according to the invention for a sufficient period to effect sustained or continuous delivery. As used herein, a “therapeutically effective dose” is a dose which in susceptible subjects is sufficient to prevent progression or cause regression of kidney disease or which is capable of relieving the symptoms caused by kidney disease.
An exemplary dosing regimen would provide the equivalent of 0.5 micrograms of calcitriol per day or at least about 1 microgram calcitriol by three times weekly. For paricalcitol, a suitable dosing regimen would provide the equivalent of about 4 micrograms paricalcitol daily or at least about 4 micrograms paricalcitol three times weekly. Suitable dosing regimens for other VDRA/Vitamin D analogs, e.g., doxercalciferol, can be determined straightforwardly by those skilled in the art based on the therapeutic efficacy of the VDRA/Vitamin D analog to be administered.
Since ACEI, ARB and aldosterone inhibitors have different efficacies and affect the body through different proteins in the RAAS pathway than a VDRA/Vitamin D does, compositions according to the present invention can incorporate an ACEI, ARB or aldosterone inhibitor to be administered according to conventional dosing regimens, which are well known and readily available to those skilled in the art.
The invention also contemplates continuous or sustained drug delivery forms containing the selected VDRA/Vitamin D analog, and an ACEI and/or an ARB and/or an aldosterone blocker. Suitable delivery forms include, but are not limited to, tablets or capsules for oral administration, injections, transdermal patches for topical administration (e.g., drug to be delivered is mixed with polymer matrix adhered to or absorbed on a support or backing substrate, e.g., ethylcellulose), depots (e.g., injectable microspheres containing the desired bioactive compounds) and implants. Techniques for making these drug delivery forms are well-known to those skilled in the art.
Recently, it has been found that 1,25-dihydroxyvitamin D functions as a negative regulator of renin biosynthesis in vitro and in in vivo studies. Calcitriol is able to inhibit renin gene expression, which provides a molecular basis to explore the use of vitamin D and vitamin D analogs as new renin inhibitor to regulate rennin-angiotensin-aldosterone system (RAAS).
Using an in vitro cell culture system, the activity of paricalcitol to suppress renin gene expression was examined using previously published techniques (1,25-Dihydroxyvitamin D3 is a negative endocrine regulator of the renin-angiotensin system, J. Clin. Invest., July 2002). As shown in
This data supports the utility of a VDRA/vitamin D analog to regulate the renin-angiotensin-aldosterone system and its criticality in CKD development and delay in progression of renal disease.
The effect of paricalcitol and calcitriol on PAI-1 in primary culture of human coronary artery smooth muscle cells was investigated. (See
Human coronary artery smooth muscle cells were incubated with paricalcitol or calcitriol at the indicated concentration for 24 hr at 37° C. Samples were solubilized in SDS-PAGE sample buffer, and the protein content in each sample was determined by the Bio-Rad dye-binding protein assay. Samples were resolved by SDS-PAGE using a 4-12% gel, and proteins were electrophoretically transferred to PVDF membrane for Western blotting. The membrane was blotted for 1 h at 25° C. with 5% nonfat dry milk in PBS-T and then incubated with a mouse anti-PAI-1 monoclonal antibody in PBS-T overnight at 4° C. The membrane was washed with PBS-T and incubated with a horseradish peroxidase-labeled anti-rabbit antibody for 1 h at 25° C. The membrane was then incubated with detection reagent (SuperSignal WestPico). The specific bands were visualized by exposing the paper to Kodak BioMax films.
These results show that paricalcitol and calcitriol are equally potent in reducing the PAI level in human coronary artery smooth muscle cells. Paricalcitol is usually dosed approximately 4 fold higher than calcitriol in the clinical situation, which may translate into a 4-fold higher potency in regulating the function of smooth muscle cells.
In fibrotic renal disease, PAI-1 is increased and localizes to areas of glomerulosclerosis. Conversely, inhibition of angiotensin or aldosterone decreases PAI-1 and also decreases renal scarring. These results show that paricalcitol is able to decrease PAI-1 level, suggesting the potential role of paricalcitol on attenuation of glomerulosclerosis.
The present application claims priority to U.S. Provisional Application No. 60/491,025, filed on Jul. 29, 2003, hereby incorporated in its entirety by reference.
| Number | Date | Country | |
|---|---|---|---|
| 60491025 | Jul 2003 | US |
