Claims
- 1. A method of using carbon dioxide in supercritical state for eliminating viral contamination from an implantable tissue, comprising the steps of:
- a) treating the tissue with a predetermined amount of supercritical carbon dioxide;
- b) treating the tissue obtained in step a) with hydrogen peroxide;
- c) treating the tissue obtained in step b) with sodium hydroxide; and
- d) treating the tissue obtained in step c) with ethanol.
- 2. A method of using carbon dioxide in supercritical state for eliminating vital contamination from an implantable tissue, comprising the steps of:
- a) treating the tissue with a predetermined amount of supercritical carbon dioxide; and
- b) treating the tissue with a predetermined amount of sodium hydroxide solution.
- 3. The method as claimed in claim 2, wherein:
- a) the tissue is treated at a rate of about 100 to about 500 grams of supercritical carbon dioxide/gram of tissue.
- 4. The method as claimed in claim 2, wherein:
- a) the tissue is treated at a rate of about 300 grams of supercritical carbon dioxide/gram of tissue.
- 5. The method as claimed in claim 2, and wherein:
- a) the tissue is treated with carbon dioxide for a period determined as a function of carbon dioxide flow rate in order to permit passage of about 100 to about 500 grams of supercritical carbon dioxide/gram of tissue.
- 6. The method as claimed in claim 2, and wherein:
- a) the tissue is treated with carbon dioxide for a period determined as a function of carbon dioxide flow rate in order to permit passage of about 300 grams of supercritical carbon dioxide/gram of tissue.
- 7. The method as claimed in claim 2, wherein:
- a) the supercritical carbon dioxide is applied at a pressure between about 1.times.10.sup.7 Pa and about 5.times.10.sup.7 Pa.
- 8. The method as claimed in claim 7, wherein:
- a) the supercritical carbon dioxide is applied at a pressure between about 2.5.times.10.sup.7 Pa.
- 9. The method as claimed in claim 2, wherein:
- a) the supercritical carbon dioxide is applied at a temperature between about 40.degree. C. and about 55.degree. C.
- 10. The method as claimed in claim 9, wherein:
- a) the supercritical carbon dioxide is applied at a temperature about 50.degree. C.
- 11. The method as claimed in claim 2, further comprising the step of:
- a) treating the tissue with a predetermined amount of hydrogen peroxide to further eliminate vital contamination from the tissue.
- 12. The method as claimed in claim 11, wherein:
- a) the hydrogen peroxide has a concentration of about 1% to about 35% by weight.
- 13. The method as claimed in claim 11, wherein:
- a) the hydrogen peroxide has a concentration of about 35% by weight.
- 14. The method as claimed in claim 11, and wherein:
- a) the tissue is treated at a temperature of about 30.degree. C. to about 50.degree. C.
- 15. The method as claimed in claim 11, and wherein:
- a) the tissue is treated at a temperature of about 40.degree. C.
- 16. The method as claimed in claim 2, further comprising the step of:
- a) treating the tissue with a predetermined amount of ethanol to further eliminate a viral contamination from the tissue.
- 17. The method as claimed in claim 16, wherein:
- a) the ethanol has a concentration greater than about 90% by volume.
- 18. The method as claimed in claim 16, wherein:
- a) the ethanol has a concentration about 95% by volume.
- 19. The method as claimed in claim 17, further comprising the step of:
- a) immersing the ethanol treated tissue in another ethanol solution with a concentration greater than about 95% by volume.
- 20. The method as claimed in claim 17, further comprising the step of:
- a) immersing the ethanol treated tissue in another ethanol solution with a concentration of about 100% by volume.
- 21. The method as claimed in claim 2, wherein:
- a) the viral contamination is a virus selected from the family group consisting of Togaviridae, Picoviridae, Herpesviridae and Retroviridae.
- 22. The method as claimed in claim 21, wherein:
- a) the viral contamination is a virus selected from the genus group consisting of Alphavirus, Enterovirus and Lentivirus.
- 23. The method as claimed in claim 22, wherein:
- a) the virus is selected from the group consisting of herpes virus, human immunodeficiency virus (HIV), Hepatitis C virus, and Polio sabin virus.
- 24. The method as claimed in claim 2, wherein:
- a) the tissue is selected from the group consisting of bone, cartilage, tendon and ligament.
- 25. A method of using carbon dioxide in supercritical state for eliminating viral contamination from an implantable tissue, comprising the steps of:
- a) treating the tissue with a predetermined amount of supercritical carbon dioxide;
- b) treating the tissue with a predetermined amount of sodium hydroxide solution; and
- c) the sodium hydroxide has a concentration between about 3% and 15%.
- 26. A method of using carbon dioxide in supercritical state for eliminating viral contamination from an implantable tissue, comprising the steps of:
- a) treating the tissue with a predetermined amount of supercritical carbon dioxide;
- b) treating the tissue with a predetermined amount of sodium hydroxide solution; and
- c) the sodium hydroxide has a concentration of about 4%.
- 27. A method of using carbon dioxide in supercritical state for eliminating viral contamination from an implantable tissue, comprising the steps of:
- a) treating the tissue with a predetermined amount of supercritical carbon dioxide;
- b) treating the tissue with a predetermined amount of sodium hydroxide solution;
- c) rising the tissue with water;
- d) immersing the rinsed tissue in a neutralizing solution; and
- e) the rinsing and immersing steps are carried out subsequent to treating the tissue with sodium hydroxide.
Priority Claims (1)
Number |
Date |
Country |
Kind |
95 07266 |
Jun 1995 |
FRX |
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Parent Case Info
This application is a continuation-in-part of U.S. application Ser. No. 08/163,775, filed Dec. 9, 1993, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4776173 |
Kamarei et al. |
Oct 1988 |
|
Foreign Referenced Citations (4)
Number |
Date |
Country |
0 603 920 A1 |
Jun 1994 |
EPX |
2695140 |
Mar 1994 |
FRX |
WO 9317724 |
Sep 1993 |
WOX |
9403590 |
Feb 1994 |
WOX |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
163775 |
Dec 1993 |
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