Patent Application
20100099756
This invention relates to uses of incensole, incensole acetate, their derivatives, and pharmaceutical compositions comprising them, for treating various diseases or conditions.
Boswellia species (Burseraceae) are native of Eastern Africa, where their resin (“frankincense” “olibanum”) has been widely used as incense and for various medical purposes. For example these species are known as diuretic agents, for the treatment of vasious diseases such as Bilharzia, stomachache syphilis and Rheumatism (Watt, 1962). Boswellia resin was found to be useful for the treatment of inflammations (Singh & Atal, 1986), as well as several diseases associated with inflammatory conditions such as for example active Crohn's disease and Asthma (Gerhardt et al., 2001; Gupta, 1998). It was previously reported that the anti-inflammatory properties of Boswellia resin may be attributed to the Boswellic acid and its derivatives (Ammon et al., 1993).
The use of Boswellia resin for its psychoactive properties extends beyond the Near East and Europe. In Ayurveda, the Indian medical tradition, Boswellia resin is reported to have a ‘strong action on the nervous system’. In Ethiopia, where Boswellia trees are indigenous, it is believed to have a tranquilizing effect.
The isolation of IA (incensole acetate) and its structural elucidation was first described by Corsano and Nicoletti (Corsano & Nicoletti, 1967). However, none of the therapeutic properties of the Boswellia resin were attributed to incensole acetate so far.
U.S. Pat. No. 5,064,823 discloses pentacyclic triterpenoid compounds such as a boswelic acid and its acetate, which have an inhibitory effect on topoisomerase I and topoisomerase II.
WO 02/053138 discloses the use of incensole and/or furanogermacrens, derivatives, metabolites and analogeous thereof for selective inhibition of neoplastic cells, for example for the treatment, inhibition or prevention of precancerous lesions, tumors, cancer growth or other neoplasias in mammals.
NF-κB (nuclear factor-κB) is a collective name for a group of inducible dimeric transcription factors. NF-κB is found in essentially all cell types and is involved in activation of a large number of genes in response to various stressful situations, e.g. infection and inflammation. The subcellular localization of NF-κB is controlled by a family of inhibitory proteins, IκBs, which bind NF-κB and mask its nuclear localization signal, thus preventing nuclear translocation. Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IκB, which frees NF-κB to translocate to the nucleus where it regulates gene transcription (Karin and Ben-Neriah, 2000). IκB phosphorylation, followed by its degradation is considered to be the major step in NF-κB regulation (Ghosh & Karin, 2002).
Traumatic brain injury (TBI) is often associated with permanent cognitive disorders, learning disabilities and various behavioral and emotional problems. Despite promising pre-clinical data, most of the clinical trials conducted so far have failed to demonstrate any significant improvement in outcomes, mainly because of ineffective therapies or because of the selection of inappropriate target mechanisms (Marmarou et al, 2005, Narayan et al, 2002). Secondary brain damage, triggered by the initial impact, develops over hours, weeks and even months following injury. Secondary brain damage can increase mortality and worsen disability but, unlike the primary lesion, may potentially be attenuated by appropriate treatment. TBI induces early phase neuronal activation of NF-κB, followed by its remarkably prolonged activation (Beni et al, 2004) even up to 1 year (Nonaka, 1999). Studies on the role of NF-κB in the brain following closed head injury in (CHI) mice have revealed that inhibition of acute NF-κB activation is associated with enhanced functional recovery (Beni et al, 2004).
The invention relates to use of a compound having the structural formula I, including enantiomers, diastereomers, solvates, and pharmaceutically acceptable salts thereof:
wherein,
The invention additionally relates to a pharmaceutical composition comprising (a) as an active ingredient a compound having a structural formula I as defined in the present invention; and (b) a pharmaceutically acceptable carrier, for the treatment, prevention or amelioration of inflammatory-associated conditions.
The invention further relates to a pharmaceutical composition consisting essentially of (a) a compound having the structural formula I as defined in the present invention; and (b) a pharmaceutically acceptable carrier, for the treatment, prevention or amelioration of inflammatory-associated conditions.
The invention additionally relates to the use of a compound having the structural formula I as defined in the present invention for the preparation of a medicament for neuroprotection. Moreover, the invention relates to the use of a compound having the to structural formula I for the preparation of a medicament for treatment, prevention or amelioration of a disease or condition selected from depression, anxiety, obsessive compulsive behaviors, deterioration in cognitive function, and deterioration in neurobehavioral function.
The invention further relates to the use of a compound having the structural formula I as defined in the present invention for the preparation of a medicament for treating a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition of the NF-κB pathway.
The invention additionally relates to the use of a compound having the structural formula I as defined in the present invention, for the preparation of a medicament for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition COX-2 activities.
The invention further relates to the use of a compound having the structural formula I as defined in the present invention, for the preparation of a medicament for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by reducing the levels of at least one of TNFα, NO, IL1, IL6, PGE2 or ROS.
Further provided by the invention, use of a TRPV3 agonist in the preparation of a medicament for treating a disease or condition selected from mood-disorders, anxiety, and a combination thereof.
Additionally the invention relates to a method of treatment, prevention or amelioration of inflammatory-associated conditions comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I, including enantiomers, diastereomers, solvates, and pharmaceutically acceptable salts thereof:
wherein,
Further, the invention relates to a method for providing neuroprotection comprising administering to a subject in need of such neuroprotection a compound having the structural formula I as defined in the present invention.
Moreover, the invention relates to a method for treatment, prevention or amelioration of a disease or condition selected from depression, anxiety, obsessive compulsive behaviors, deterioration in cognitive function, and deterioration in neurobehavioral function, comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined the present invention.
The invention further relates to a method for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition of the NF-κB pathway comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined in the present invention.
The invention additionally relates to a method for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition COX-2 activities comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined in the present invention.
Further, the invention relates to a method for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by reducing the levels of at least one of TNFα, NO, IL1, IL6, PGE2 or ROS comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined in the present invention.
Additionally, the invention relates to a method for treatment of a disease or condition selected from mood-disorders, anxiety, and a combination thereof, comprising administering to a subject in need of such treatment a therapeutically effective amount of TRPV3 agonist.
In order to understand the invention and to see how it may be carried out in practice, embodiments will now be described, by way of non-limiting example only, with reference to the accompanying drawings, in which:
The present invention is based on the finding that incensole (IN) and incensole acetate (IA), possess various pharmacological activities which were not previously attributed to the isolated compounds per-se.
In the first aspect of the invention, there is provided a use of a compound having the structural formula I, including enantiomers, diastereomers, solvates, and pharmaceutically acceptable salts thereof:
wherein,
By the term “one of R1 and R2 and one of R3 and R4 taken together form a second bond between C12 and C13” is meant that the bond formed between C12 and C13 is a it bond, thereby the bond between C12 and C13 is a double bond.
Similarly by the term “one of R5 and R6 and one of R7 and R8 taken together form (iii) a second bond between C8 and C9” is meant that the bond formed between C8 and C9 is a π bond, thereby the bond between C8 and C9 is a double bond.
It is appreciated that double bond conformations are also within the scope of the present invention.
In a specific embodiment of the present invention, the compound is of structural formula II, including enantiomers, diastereomers, solvates, and pharmaceutically acceptable salts thereof:
wherein,
for the preparation of a medicament for treatment, prevention or amelioration inflammatory-associated conditions.
As used herein the term “C1-25 alkyl” refers to a saturated aliphatic hydrocarbon of 1 to 25 carbon atoms. The C1-25 alkyl may be a straight or a branched allyl.
Whenever a numerical range e.g. “1-25” is stated herein, it means that the group in this case the alkyl group, may contain 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc., up to and including 25 carbon atoms.
According to one embodiment of the present invention, the compound is incensole or incensole acetate. The structures of these compounds are shown below.
As used herein, the term “inflammatory-associated condition” refers to any disease or pathologically condition which can benefit from the reduction of at least one inflammatory parameter. The condition may be caused (primarily) from inflammation, or inflammation may be one of the manifestations of the diseases caused by another physiological cause.
The term “treatment, prevention or amelioration” in connection with the inflammatory disease aspect concerns improvement of at least one undesired manifestation of the disease such as: increase in disease free periods, decrease in acute disease periods (in time and severely), decrease in severity of the disease, improvement in life quality, decreased mortality as well as prophylactic treatment before disease occurs.
In a further embodiment of the invention, said inflammatory associated condition is selected from: rheumatoid arthritis, inflammatory bowel disease, systemic lupus erythematosus (SLE), psoriasis, Type I diabetes (IDDM), Sjogren's syndrome, autoimmune thyroid disease, sarcoidosis, autoimmune uveitis, autoimmune hepatitis, hypersensitivity lung diseases, hypersensitivity pneumonitis, delayed-type hypersensitivity, interstitial lung disease (ILD), scleroderma, dermatitis, iritis, conjunctivitis, keratoconjunctivitis, cutaneous lupus erythematosus, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia, polychondritis, Graves opthalmopathy, amyotrophic lateral sclerosis (ALS), primary biliary cirrhosis, ileitis, chronic inflammatory intestinal disease, celiac disease, irritable bowel syndrome, neurodegenerative diseases, ataxiatelangiectasia, asthma, psoriasis, atherosclerosis, and combination of any of the above.
Examples of inflammatory bowel disease are Crohn's and ulcerative colitis. Examples of interstitial lung disease (ILD) are idiopathic pulmonary fibrosis, or ILD associated with rheumatoid arthritis or other inflammatory diseases.
Dermatitis may be for example atopic dermatitis or eczematous dermatitis. The neurodegenerative disease may be for example MS (multiple sclerisis).
Additional diseases/conditions are described in Barnes & Karin M., 1997 (psoriasis), Hansson, 2001 (atherosclerosis), Foo S. Y. & Nolan, 1999 (autoimmunity diseases).
A specific example of inflammatory associated condition is rheumatoid arthritis.
As used herein the term “medicament” refers to a pharmaceutical composition. Specifically, it refers to a pharmaceutical composition comprising at least one compound of structural formula I described in the present invention in any suitable pharmaceutical acceptable carrier (e.g. an excipient or diluent), and also to different formulations required for different routes of administration. For example the medicament may be formulated for oral administration, or may be formulated for parenteral, rectal or other modes of administration.
The active ingredients of a pharmaceutical composition as disclosed herein may include at least one compound of formula I, i.e. a single compound, or two or more compounds.
In a further aspect of the invention there is provided a pharmaceutical composition comprising (a) as an active ingredient a compound having the structural formula I as defined herein above; and (b) a pharmaceutically acceptable carrier, for the treatment, prevention or amelioration of inflammatory-associated conditions.
By another one of its aspects, the invention provides a pharmaceutical composition consisting essentially of (a) as an active ingredient a compound having a structural formula I as defined hereinabove; and (b) a pharmaceutically acceptable carrier, for the treatment, prevention or amelioration of inflammatory-associated conditions.
The pharmaceutical compositions are further described below.
By the term “consisting essentially of” in connection with a pharmaceutical composition is meant that the active ingredient includes one or more compounds of formula I as defined above and is substantially free of other active compounds. By the term “substantially free of other active compounds” is meant that the active ingredient includes at least 70% w/w of a compound of formula I, more preferably at least 80% w/w, more preferably at least 90% w/w, even more preferably at least 95% w/w of a compound of formula I. The active ingredient may include at least one of the above indicated concentrations of compound of formula I and up to 99.9% w/w of compound of formula I. The active ingredient may also include at least one of the above indicated concentrations and up to 99% w/w of compound of formula I.
By yet a further aspect of the invention there is provided a use of a compound having the structural formula I as hereinabove defined for the preparation of a medicament for neuroprotection.
In one embodiment said neuroprotection is for treatment, prevention or amelioration of a disease or condition resulting from injury, trauma, or CNS neurodegenerative diseases.
The term “treatment, prevention or amelioration” in connection with neuroprotection as used herein, means treating, preventing, or reversing cognitive decline associated with concentration loss, memory-acquisition loss, and information-storage or retrieval loss including, but not limited to, neuronal disorders, such as cognitive decline associated with aging, cognitive impairment and neurodegenerative disorders, such as Alzheimer's disease, Parkinson's disease, ALS, Huntington Chorea, HIV associated dementia, Lewy body dementia, multiple sclerosis, and prion disease. The term also includes treating, preventing, or reversing neuronal dysfunction associated with loss of motor skills (ataxia), such as Parkinson's disease and amyotrophic lateral sclerosis as well as neuronal dysfunction resulting from CNS injury, such as head trauma, stroke, spinal-cord injury, and peripheral-nerve injury.
As used herein the term “neurodegenerative disease” refers broadly to disorders or diseases that affect the nervous system and are characterized by gradual neuronal loss and/or gradual loss of neuronal function, including but are not limited to age-associated memory impairment, Parkinson's disease, Alzheimer's disease, Huntington's chorea disease, multiple sclerosis and amyotrophic lateral sclerosis (ALS), HIV associated dementia, Lewy body dementia, and prion disease.
In another one of its aspects the present invention provides a use of a compound having the structural formula I for the preparation of a medicament for treatment, prevention or amelioration of a disease or condition selected from depression, anxiety, obsessive compulsive behaviors, deterioration in cognitive function, deterioration in neurobehavioral function, and combination of any of the above.
The term “deterioration of cognitive and/or neurobehavioral function” refers to decrease in learning and memory capacitates, to decrease in orientation in time and space and decrease in coordination, and movement capacities due to CNS function. The deterioration may be a natural result of aging but may also be as a result of injury, trauma (caused by accidents, stroke, surgery or diseases) or of disease in the CNS notably neurodegenerative diseases.
The terms “injury” and “trauma” includes physical injury to the CNS (or head) as a result of physical insult, injury or damage due to stroke, ischemia , hypoxia, surgery or a disease such as an infectious disease in the CNS (such as AIDS-associated dementia) as well as a neurodegenerative disease, for example Alzheimers, Parkinson, Hungtinton Chorea or old age dementia.
The term “treatment, prevention or amelioration of depression, anxiety or obsessive compulsive behavior” refers to decrease or elimination of the severity of the condition, decrease in the duration of the episode as well as preventive treatment in individuals prone for such conditions to avoid or minimize the entry to these undesired episodes. The term “treatment” in connection with depression concerns improvement of at least one undesired manifestation of the disease such as anorexia and bulimia as well as the manifestation of clinical depression.
In a further aspect, the invention provides a use of a compound having the structural formula I as hereinabove defined for the preparation of a medicament for treating a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition of the NF-κB pathway.
The invention further provides a use of a compound having the structural formula I as hereinabove defined, for preparation of a medicament for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition COX-2 activities.
By another aspect the invention provides a use of a compound having the structural formula I as hereinabove defined, for the preparation of a medicament for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by reducing the levels of at least one of TNFα, NO, IL1, IL6, PGE2 or ROS.
The term “beneficial clinical outcome is achieved” refers to diseases or pathological conditions, for which it is accepted in the medicinal community that a desired clinical result can be achieved by administration to patients of agents that inhibit the NF-κB pathway, inhibit COX-2 activity or reduce the level of at least one of the following: TNFα, NO, IL1, IL6, PGE2 or ROS in the subject as compared to non treated control.
It is demonstrated in the present invention that incensole acetate (IA), a Boswellia resin constituent, is a potent TRPV3 agonist that causes anxiolytic-like and antidepressive-like behavioral effects in wild type (WT) mice with concomitant changes in c-Fos activation in the brain. These behavioral effects were not noted in TRPV3−/− mice, suggesting that they are mediated via TRPV3 channels. IA robustly activated TRPV3 channels stably expressed in HEK293 cells and in keratinocytes from TRPV3+/+ mice. It had no effect on keratinocytes from TRPV3−/− mice and showed modest or no effects on TRPV1, TRPV2 and TRPV4. The results shown below (see Example 15) imply that TRPV3 channels in the brain play a role in emotional regulation.
In a further aspect of the invention, there is provided a use of a TRPV3 agonist for the preparation of a medicament for treating a disease or condition selected from mood-disorders, anxiety, and a combination thereof.
As used herein the term “mood disorders” refers to an emotional and/or behavioral disturbance characterized by persistent and pervasive bouts of euphoria and/or depression. Exemplary mood disorders include depression and bipolar disorders (also known as manic depressive illness). Anxiety is frequently associated with mood disorders, such as depression.
By a specific embodiment the mood-disorder is depression.
In one embodiment, said TRPV3 agonist is a compound having the structural formula I as defined hereinabove. In yet a further embodiment said compound is incensole or incensole acetate.
According to another embodiment the TRPV3 agonist is a monoterpenoid such as described in AK Vogt-Eisele et al., Monoterpenoid agonists of TRPV3, British Journal of Pharmacology (2007) 151, 530-540; Haoxing Xu et al. Nature Neuroscience (2006) 9, 628-635.
Non limiting examples include camphor, thymol, carvacrol, and euginol.
An additional example is 2-aminoethyl diphenylborinate (2-APB).
The invention further relates to a compound of structural formula I for the preparation of a medicament useful as a TRPV3 agonist.
The invention additionally relate to the use of a compound having structural formula I for the preparation of a medicament for treatment, prevention, or amelioration of a disease or condition selected mood disorders, anxiety, and a combination thereof.
By yet another aspect the present invention is based on the finding that IA is anti-proliferative. This finding may lead to the use of IA as an anti-proliferative agent mainly for the treatment of cancer as well as other proliferative diseases. Thus, the pharmaceutical composition of the invention may be for the treatment of hyperproliferative disorders such as carcinomas and lymphomas preferably of hyperproliferative disease in cancer of haematopoeitic origin. Alternatively, the pharmaceutical composition of the invention may be for the treatment of a non-malignant hyperproliferative disorder, for example psoriasis.
Specifically, as IA, IN and their derivatives are anti-proliferative as well as anti-inflammatory agents, they are of great potential value for the treatment of psoriasis.
Thus, the invention additionally relates to the use of a compound of structural formula I for the preparation of a medicament for the treatment of hyperproliferative disease or disorder.
As used herein, the term “treating” in the context of the hyperproliferative disease or disorder refers to alleviating or diminishing a symptom associated with a cancerous disease. Preferably, treating cures, e.g., substantially eliminating the symptoms associated with cancer. The term “treating” may refer to decrease in tumor load, decrease in metastasis, slowing of tumor progression, slowing in metastasis formation, slowing the advancement from one tumor stage to the other , improving life quality decreasing mortality. The treatment may also be prophylactic treatment before the tumor occurs.
In a further aspect of the invention, there is provided a method of treatment, prevention or amelioration of inflammatory-associated conditions comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I, including enantiomers, diastereomers, solvates, and pharmaceutically acceptable salts thereof, as defined herein above.
The term “subject” refers to any animal, preferably a mammal.
As used herein the term “mammal” refers to any member of the class Mammalia, including a human. Preferably, the mammal herein is human.
In another aspect of the invention, a method is given for providing neuroprotection comprising administering to a subject in need of such neuroprotection a compound having the structural formula I as defined herein above.
In a further aspect of the invention there is provided a method for treatment, prevention or amelioration of a disease or condition selected from depression, anxiety, obsessive compulsive behaviors, deterioration in cognitive function, and deterioration in neurobehavioral function, comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined hereinabove.
In another aspect of the invention, there is provided a method for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition of the NF-κB pathway comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined hereinabove.
In a further aspect of the invention there is provided a method for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition of COX-2 activities comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined hereinabove.
In yet another aspect of the invention, there is provided a method for the treatment of a disease or condition wherein a beneficial clinical outcome is achieved by reducing the levels of at least one of TNFα, NO, IL1, IL6, PGE2 or ROS comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined hereinabove.
In another aspect of the invention, there is provided a method for treatment of a disease or condition selected from mood-disorders, anxiety, and a combination thereof, comprising administering to a subject in need of such treatment a therapeutically effective amount of TRPV3 agonist.
In a further aspect of the invention, there is provided a method for treatment of a disease or condition selected from mood-disorders, anxiety, and a combination thereof, comprising administering to a subject in need of such treatment a therapeutically effective amount of a compound having the structural formula I as defined hereinabove.
According to an additional aspect of the present invention, there is provided a pharmaceutical composition comprising (a) as an active ingredient a compound having a structural formula I as defined in the present invention; and (b) a pharmaceutically acceptable carrier, for the treatment, prevention or amelioration of one or more of the following diseases or conditions:
(i) inflammatory-associated conditions;
(ii) a disease or condition where neuroprotection is required;
(iii) a disease or condition selected from depression, anxiety, obsessive compulsive behaviors, deterioration in cognitive function, and deterioration in neurobehavioral function;
(iv) a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition of the NF-κB pathway;
(v) a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition COX-2 activities;
(vi) a disease or condition wherein a beneficial clinical outcome is achieved by reducing the levels of at least one of TNFα, NO, IL1, IL6, PGE2 or ROS.
According to a further aspect of the present invention there is provided a pharmaceutical composition consisting essentially of (a) as an active ingredient a compound having a structural formula I as defined in the present invention; and (b) a pharmaceutically acceptable carrier, for the treatment, prevention or amelioration of one or more of the following diseases or conditions:
(i) inflammatory-associated conditions;
(ii) a disease or condition where neuroprotection is required;
(iii) a disease or condition selected from depression, anxiety, obsessive compulsive behaviors, deterioration in cognitive function, and deterioration in neurobehavioral function;
(iv) a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition of the NF-κB pathway;
(v) a disease or condition wherein a beneficial clinical outcome is achieved by the inhibition COX-2 activities;
(vi) a disease or condition wherein a beneficial clinical outcome is achieved by reducing the levels of at least one of TNFα, NO, IL1, IL6, PGE2 or ROS.
According to a further aspect of the present invention, there is provided a pharmaceutical composition comprising (a) a compound having a structural formula I as defined in the present invention; and (b) a pharmaceutically acceptable carrier, for providing a neuroprotective effect.
According to a further aspect of the present invention there is provided a pharmaceutical composition consisting essentially of (a) a compound having a structural formula I as defined in the present invention; and (b) a pharmaceutically acceptable carrier, for providing a neuroprotective effect.
In a specific embodiment (for the uses, methods, and pharmaceutical compositions described in the present invention), the compound of structural formula I is of structural formula II as defined in the present invention, and in a more specific embodiment the compound is incensole or incensole acetate.
In one embodiment R′ and/or R″ of structural formula I are each independently C1-20alkyl; in a further embodiment C1-15alkyl; in yet a further embodiment C1-5alkyl; in a further embodiment C1-4alkyl; in an additional embodiment C1-5alkyl.
In another embodiment, the bond between carbons 8,9 and\or 12,13 is a single bond. In a further embodiment carbons 8,9 and\or 12,13 form an epoxide ring, along with the carbon to which they are bonded. In yet a further embodiment, the substituents on carbons 8,9 and\or 12,13 are substituted as to form a diol. In another embodiment one or more of R1, R2, R5, R6, and R9 is H.
In a specific embodiment of the present invention, the compound of the invention is of structural formula II, including enantiomers, diastereomers, solvates, and pharmaceutically acceptable salts thereof:
wherein,
In one embodiment of the present invention, the compound is incensole or incensole acetate.
Compounds used by the methods and uses of the invention may be synthesized by the synthetic routes described and detailed in G. STRAPPAGHETTI, G. PROIETTI, S. CORSANO, AND I. GRGURINA. Synthesis of incensole. BIOORGANIC CHEMISTRY 11, 1-3 (1982) and T. Kato, C. C. Yen, T. Kobayashi, Y. Kitahara. Cyclization of polyenes XXI. Synthesis of DL-incensole. Chemistry letters 1191-1192 (1976), which are herein incorporated by reference in their entirety. The derivatives of structural formula I may be synthesized by procedures as described in Fessenden R. J. & Fessenden J. S.; Organic chemistry, 1990, Brooks/Cole Publishing company, California (pp. 257-301 (alcohols), 301-323 (ethers and epoxides), 529-591 (aldehydes and ketones), 591-627 (Derivatives of carboxylic acids), 391-448 (double bonds)). Synthesis procedures can be also found in additional general textbooks, for example, Morrison R. T. & Boyd R. N.; Organic chemistry, 1992, Pramount communication company, California.
As used herein a “pharmaceutical composition” refers to a preparation of one or more compounds described herein, with other inert chemical components such as suitable pharmaceutically acceptable carriers. The purpose of a pharmaceutical composition is to facilitate administration of a compound to a mammal. As used herein the term “pharmaceutically acceptable carrier” refers to an inert non-toxic carrier or diluent that does not cause significant irritation to a subject (mammal) and does not abrogate the biological activity and properties of the administered compound.
Examples without limitation of carriers are lactose, sucrose, water, organic solvents, and polyethyleneglycol.
The carriers may include additional excipients such as binders, disintegrants, lubricants, surface active agents, preservatives and favoring agents. According to one embodiment of the present invention the route of administration of the composition is selected from oral, parenteral, inhalation, topical, transdermal, nasal, transmucosal (e.g. intranasal), intestinal, and rectal.
Additionally according to a preferred embodiment of the present invention the parenteral route of administration is selected from intravenous, intramuscular, intraperitoneal and subcutaneous administration.
Additional suitable routes may be for example intramedullary, intrathecal, direct intraventicular, and intraocular injections.
A specific embodiment is the oral route of administration.
The pharmaceutical composition of the present invention may be formulated as to provide immediate release or sustained release of the active ingredient from the dosage form after administration to a patient by employing procedures well known in the art.
The final form of the composition includes but not limited to a liquid, a syrup, an elixir, an emulsion, a suspension, drops, a spray, a cream, an ointment, a lotion, a gel, a paste, a powder, a granule, a tablet, a caplet, a pill, a capsule, a suppository, a transdermal patch or an injection.
The pharmaceutically acceptable carrier selected for preparing the pharmaceutical compositions of the present invention depends on the final form of the composition.
Typically, such carriers include additional excipients such as binders, disintegrants, adsorbents, lubricants, wetting agents, buffering agents and surface active agents.
The pharmaceutical compositions of the present invention are preferably present in a unit dosage form. Unit dosage form as used herein refers to physically discrete units suited as unitary dosages for the mammalian subject to be treated, such as a tablet, a capsule, or powders in vials or ampoules, each unit containing a predetermined quantity of the active ingredient calculated to produce the desired therapeutic effect.
Preferably the pharmaceutical composition in a unit dosage form comprises a therapeutically effective amount of the active ingredient in an amount from 0.1 mg to 1000 mg, more preferably 1 to 500 mg.
Oral dosage forms of the present invention suitable for oral administration may be presented as discrete pharmaceutical unit dosage forms, such as capsules, cachets, soft elastic gelatin capsules, tablets, caplets, or aerosols sprays, each containing a predetermined amount of the active ingredients, as a powder or granules, or as a solution or a suspension in an aqueous liquid, a non-aqueous liquid, an oil-in-water emulsion, or a water-in-oil liquid emulsion. Dosage forms such as oil-in-water emulsions typically comprise surfactants such as an anionic surfactant, for example anionic phosphate ester or lauryl sulfates, but other types of surfactants such as cationic or nonionic surfactants may be used in the compositions of the present invention. See generally, Remington's Pharmaceutical Sciences, Mack Publishing, Easton Pa., latest edition.
For the purpose of preparing a tablet dosage form, various pharmaceutical carriers which are well-known in this field can be widely used. As to the examples of carriers, excipients such as lactose, sodium chloride, glucose, starch, calcium carbonate, kaolin, cellulose, aluminum silicate and the like may be used; the binders may be for example water, ethanol, propanol, glucose solution, starch solution, gelatin solution, carboxymethyl cellulose, shellac, methyl cellulose, polyvinylpyrrolidone and the like; the disintegrants may be for example starch, sodium alginate, sodium laurylsulfate, sodium starch glycolate and the like; the wetting agents may be for example glycerin, surfactants and the like; the adsorbents may be for example starch, lactose, kaolin, bentonite, colloidal silicic acid and the like; lubricants such as talc, strearates, polyethylene glycols and the like can be used. The tablets preparations can be further shaped into tablets coated with usual tablet coating, for example sugar coated tablets, gelatin film coated tablets, tablets coated with enteric coating, tablets coated with film coating, or double layer tablets and multiple layer tablets.
For the purpose of preparing a capsule dosage form, the compounds of formula [I] as the active ingredients are mixed with the above-mentioned various carriers and the mixture or granules prepared from the mixtures are placed into rigid gelatin capsules or soft capsules.
For the purpose of preparing a suppository dosage form, various carriers which are well-known in this field can be widely used. As to the examples of carries, polyethylene glycols, cacao butter, higher alcohols, esters of higher alcohols, gelatin, semi-synthesized glycerides and the like can be mentioned.
For the purpose of preparing an injection dosage form, liquid preparations, emulsion preparations and suspension preparations are sterilized, further these preparations are preferably isotonic to the blood, and all the diluents which are conventionally used in this field can also be used for example, water, ethyl alcohol, macrogols, propylene glycol, ethyoxylated isostearyl alcohol, polyoxylated isostearyl alcohol and polyoxyethylenesorbitan fatty acid esters.
Additionally, for the purpose of preparing an isotonic injection solutions, an adequate amount of sodium chloride, glucose or glycerin may be added to the injection preparations, further, usual dissolving additives, buffering agents, preservatives and the like may be added.
An example of a pharmaceutical carrier for preparing an injection emulsion preparation is triglyceride emulsion. An example of an acceptable triglyceride emulsion useful in the intravenous and intraperitoneal administration of the compounds of the present invention is the triglyceride emulsion commercially distributed under the tradename Intralipid®.
Moreover, if necessary, coloring agents, preservatives, spices, flavors, sweetening agents and others may be added to the pharmaceutical preparations of the present invention.
Topical preparations such as creams, ointments, pastes, gels, lotions, transdermal patches, inhalants, sprays, aerosols and the like are formulated by using carriers and excipients which are well known in the field.
Methods of preparing the compositions of the present invention include the step of bringing into association a compound of the present invention with the pharmaceutical carrier. In general, the compositions are prepared by uniformly and intimately bringing into association a compound of the present invention with liquid, semi-solid or solid carriers, and then, if necessary, shaping the product.
The pharmaceutical compositions of the invention may be prepared by methods of pharmacy well known to those skilled in the art, e.g. by means of conventional mixing, dissolving, pulverizing, granulating, compressing, emulsifying, levigating, or lyophilizing processes. Techniques for formulation and administration of drugs may be found in “Remington's Pharmaceutical Sciences,” Mack Publishing Co., Easton, Pa., latest edition, which is incorporated herein by reference.
Pharmaceutical compositions for use in accordance with the present invention may thus be formulated in conventional manner using one or more pharmaceutically acceptable carriers comprising excipients and auxiliaries, which facilitate processing of the active compounds into preparations which, can be used pharmaceutically. The proper formulation is dependent upon the route of administration chosen.
The amount of the active ingredient that may be combined with the pharmaceutical carrier to produce a single dosage form will vary depending upon the mammal treated and the particular mode of administration. For example, a composition intended for oral administration to humans may vary from about 5% to about 95% w/w of the total composition.
Dosage unit forms will generally contain between 0.1 to 1000 mg of the active ingredient, more preferably 1 to 500 mg.
The therapeutically or prophylactically effective amount of an active ingredient administered orally may range from 0.1 to 1000 mg daily, more preferably from 1 to 500 mg daily, either singly or in multiple dosage over 24-hour period. For oral administration, the therapeutically effective amount of the active ingredient may be several times greater than that for parenteral administration.
The above dosages refer to humans.
The desired dose is suitably administered once daily, or several sub-doses, e.g. 2 to 4 sub-doses, are administered at appropriate intervals through the day, or other appropriate schedule.
In the practice of the invention the amount of the compound incorporated in the pharmaceutical composition may vary widely. Factors considered when determining the precise amount are well known to those skilled in the art. Examples of such factors include, but are not limited to, age, sex and weight of the subject being treated, intended medical use of the compounds, severity of the disease, the dosage form, route of administration being employed and the frequency with which the composition is to be administered.
The exact dose may be determined, in accordance with the standard practice in the medical arts of “dose titrating” the recipient; that is, initially administering a low dose of the compound, and gradually increasing the dose until the desired therapeutic effect is observed.
The ratio between toxicity and therapeutic effect for a particular compound is its therapeutic index and can be expressed as the ratio between LD50 (the amount of compound lethal in 50% of the population) and ED50 (the amount of compound effective in 50% of the population). Therapeutic index data obtained from animal studies can be used in formulating a range of dosages for use in humans. The dosage of such compounds preferably lies within a range of plasma concentrations that include the ED50 with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. The exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition.
Extraction and Isolation of IA. Boswellia carterii resin (20 gr., Pamir, Tel Aviv, Israel) was extracted with PE (PE) (3 times with 150 ml). Petroleum ether (PE) extract was washed with NaOH 5% solution (3 times with 200 ml). The resulting aqueous acid-containing fraction was then acidified with HCl 1M, washed with saturated NaCl and re-extracted with PE. It was then dried over MgSO4. The non acid containing PE fraction was acidified with HCl (1M) and then washed with a saturated NaCl solution and dried over MgSO4. After evaporation the residue was chromatographed on a silica column. Fractions were assayed for their activity on IκB degradation as described below. A fraction eluted with 3% diethyl-ether in PE, which contained IA, showed activity. Pure IA was obtained by chromatography on a semi preparative HPLC column (Spectra-physics applied bio systems 783 absorbance detector with a vydac C18 semi-preparative HPLC column-Valco). Acetonitrile (ACN) and water were used as mobile phase for HPLC and the gradient consisted of 90-99% ACN for 30 min. A Waters HPLC instrument: pump 600, PDA 996 detector 600 with an analytical C18 Symmetry column (4.6/250 mm) were used to analyze the purification process. Several NMR methods (H-NMR, C-NMR, DEPT, COSY, HSQC, HMBC, TOCSY and NOESY) as well as a GC-MS analysis were used for the structure elucidation of the isolated active compounds.
NMR spectra were recorded both in CDCl3 and in C6D6 solutions using a Bruker avance spectrometer 400 MHz and repeated using a Varian Unity Spectrometer Varian Unity Inova spectrometer 500 MHz.
GC-MS Analysis was performed using a Hewlett-Packard G1800A GCD system with a HP5971 gas chomatograph with an electron ionization detector. An SPB-5 (30 m×0.25 mm×0.25 μm film thickness) column was used. The following method was used for analysis: The column was held at 70° C. for 4 mins, after which, a temperature gradient was applied from 70° C. to 280° C., at a rate of 50 degree/min. (Inlet temperature: 280° C.; Detector temperature: 280° C.; Splitless injection; gas—Helium, 1 mL/min).
Cell Cultures. HeLa cells were grown in Dulbecco's modified Eagle medium supplemented with 10% foetal calf serum and 1% (v/v) penicillin/streptomycin (all from Biological Industries, Kibbutz Beit Haemek, Israel), in a humidified incubator at 37° C.
RAW 264.7 macrophage cell line derived from BALB/c mice was obtained from American Type Culture collection (Rockville, Md., USA). The cells were cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal calf serum (Hyclone, Logan, Utah), 1% (v/v) penicillin/streptomycin (Beit Haemek, Israel), nonessential amino acid (Sigma, St. Louis, USA), glutamine 1% (Beit Haemek, Israel) and pyruvate 1% (Beit Haemek, Israel). Cells were grown in a humidified incubator at 37° C.
Peritoneal macrophages were harvested from C57BL/6 female mice four days after intraperitoneal injection of 1.5 ml of a 3% thioglycollate medium (Difco, N.J., USA). The cells were re-suspended in Dulbecco's modified Eagle medium (DMEM) supplemented with 5% foetal calf serum (FCS), and plated (1.2×105 cells per well) in 96-microwell plates flat-bottom (Nunc, Roskide, Denmark).
IκBα Degradation. HeLa cells were pre-incubated with IA (50 μg/ml, dissolved in ethanol) for 2 hrs, and then stimulated for 20 minutes with TNFα (20 ng/ml, Emeryville, Calif., USA). After removing the slides from plates for immonostaining (see below), proteins were extracted from remaining cells in the plates. Proteins were extracted from cells in NP-40 lysis buffer. Total protein concentration was determined using the Bradford method. Lysates were then analyzed either by Western blotting (WB).
Western Blot (WB). Following separation by SDS-PAGE, proteins were blotted to a polyvinylidene difluoride (PVDF) membrane (Millipore). The membrane was blocked in 5% (w/v) milk powder and then incubated in TBST containing the primary antibody and 2% (w/v) milk powder. All phospho-specific antibodies were purchased from Cell signaling Inc. αIκBa, αp65 and αCOX2 antibodies from Santa Cruz Inc. (California, USA). After binding of an appropriate secondary antibody coupled to horseradish peroxidase, proteins were visualized by enhanced chemiluminiscence according to the instructions of the manufacturer (Amersham Lifescience).
p65 Subunit Immunostaining. HeLa Cells were preincubated with IA and then stimulated with TNFα as described in the IκBα degradation assay above. Cells were then fixed with formaldehyde 1%, permeabilized with 0.25% Triton X-100, stained with rabbit anti-p65 (Santa Cruz, Calif., USA) and visualized with anti-rabbit Rhodamine Red-labeled secondary antibody (Jackson ImmunoResearch, Baltimore, USA). Cells were also stained with DAPI (blue) for nuclei location (data not shown). The cells were examined under an Axioscope Zeiss microscope with a plan-Neofluor * 60 lens.
COX-2 Production. RAW 264.7 cells were treated with subtoxic concentrations (confirmed by MTT colorimetric assay) of incensole acetate (10-20 μg/ml, dissolved in ethanol and further diluted in medium) and incubated with lipopolysaccharide (LPS, E. coli 1 μg/ml for 24 hs, Sigma, Israel) for 16-24 hrs. Cells treated with vehicle served as control group.
Proteins were extracted from cells in NP-40 lysis buffer (50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1% SDS, 1% NP-40, 10 mM EDTA, 1 mM phenylmethylsulfonylfluoride (PMSF), and 10 mM DTT). Total protein concentration was determined using the Bradford method and the lysates were analyzed by Western blotting.
Nitric Oxide (NO) Levels. Following 2-3 h of incubation, of murine peritoneal macrophages at 37° C., the non-adherent cells were removed by intensive rinsing. About 95% of the adherent cells were macrophages. IA was first dissolved in absolute ethanol, and the solutions were further diluted with Dulbecco's Modified Eagle's Medium (DMEM medium). Various nontoxic concentrations were added to the macrophages, followed by addition of 1 μg/ml of LPS for activation. The macrophages were then cultivated in a humid atmosphere with 5% CO2 for 24 hrs. The supernatant fluids were harvested and kept at −20° C. until assayed. NO generation was determined by measuring the nitrite accumulated in the supernatants (100 μl) of the IA-treated macrophages as follows. The cells were then treated with IA in various doses. An equal volume (100 μl) of Griess reagent (1% sulphanilamide, 0.1% naphthalene diamine HCl, 2% H3PO4) was added to each supernatant. Following 10 min of incubation at room temperature, the color production was measured at 550 nm with an ELISA reader. The concentration of the nitrite was calculated according to a standard curve.
ROS (Reactive Oxygen Species) Production by RAW 264.7 Macrophages. Raw 264.7 cells were scrapped, washed and resuspended in Hanks' balanced salt solution (without phenol red). For measurement of chemiluminescence, 0.5 ml of cell suspension (5×105 cells) was added to each luminometer tube, together with various doses of IA tested (dissolved in ethanol and further diluted with Hanks). The cells were incubated for 24 hrs. 10 μl of luminol (Sigma, St. Louis, USA) and 30 μl of zymosan (Sigma, St. Louis, USA) were added to the tubes, and the chemiluminescence was measured immediately in a luminometer (Biolumate LB 95, Berhold, Wilbad, Germany).
Inflamed Paw Model. Sabra female mice were used to assess the response to IA or vehicle in an in vivo model of inflammation. Drug or vehicle was administered 30 min before induction of the inflammatory stimulus. Mice (5 per group) were injected i.p. with vehicle (isopropanol:Emulphor:saline=1:1:18) or with vehicle containing IA (50 mg/kg, i.p). Emulphor (a polyethoxylated vegetable oil) is a commercial emulsifier. Hind paws were injected with 50 μl of saline (left or right alternatively) or λ-carrageenin (4%, right or left alternatively), using 26G needles. Ensuing inflammatory swelling was measured by increase in foot volume in a plethysmometer (Ugo-Basile, Italy). Paw volume as well as redness (as a measure of erythema) and licking (as a measure of pain) were assayed before carrageenin application and every 60 min until 4 hrs.
Statistical Analysis. Student's t test was used to assess the differences between the control and IA-treated groups. For a dose response effect, analysis of the data was performed using a one way ANOVA followed by Bonferroni post-hoc comparisons. The paw model results were analyzed by ANOVA followed by Bonferroni post-hoc comparisons at every time point.
For analysis of c-Fos immunoreactivity, positive nuclei were identified based on their round form and optical density at least twice that of background. The numbers of c-Fos immunoreactive nuclei from the right and left hemispheres were averaged to obtain a representative number for the given region from each mouse. Student t tests were performed comparing the control (vehicle) with the IA group.
Responses to IA in WT versus TRPV3−/− mice were assessed using two-way analysis-of-variance (ANOVA) with Bonferroni post-hoc comparisons (Graphpad Prism 4 software).
Animals and Procedures
Female Sabra mice (Harlan, Israel, 2.5-3.5 months old) were used for the paw inflammatory model. Female Sabra mice (Harlan, Israel, 15-20 weeks old) and wild type C57BL/6 or TRPV3 KO female mice (18-20 weeks old) were used for behavioral assessments. Ten mice were housed in each cage. For the chronic studies, mice were housed in groups of eight. Temperature in the animal room was maintained between 20-22° C., the light cycle was 12 h lights on (8-20 h); 12 h lights off (20-8.00 h). Female mice were used for all behavioural assessments, in order to prevent confounding due to potential wound infliction induced by inter-male fighting (See also below “Animals and Procedures” Section relating to Example 15.
Mice were consecutively tested in the elevated plus maze and the forced swimming test. The animal care and the protocols met the guidelines of the U.S. National Institutes of Health, detailed in the Guide for the Care and Use of Laboratory Animals, and were applied in conformity with the Institutional Ethics Committee.
Drugs and Injections for Behavioral Assays
IA, IN and the extract were dissolved in a mixture of isopropanol: cremophorsaline=1:1:18. Injection volume was 10 μl/g body weight. Injections were performed by the intraperitoneal (i.p.) route.
Behavioral Assays
Elevated Plus Maze
Mice were placed in the central platform (10×10 cm) between the open (10×45 cm) and enclosed (10×45×40 cm) arms of a plus maze. The number of entries and the time spent in each of the arms was recorded. As described by others (Crawley, 2000; Treit and Menard, 1998), an ‘anti-anxiety’ effect was calculated both as the ratio of entries onto the open arms to total arm entries, and as the % time on the open arms proportional to the time in the closed arms. Mice (female Sabra strain, aged 3.5-4.5 months old) were injected intraperitoneally with 10, 30 or 50 mg/kg of incensole acetate or with vehicle. Each dose was administered to 5 mice. Fifty min after injection the mice were tested in the plus-maze for ‘anti-depressant’ effects. Desipramine (5 mg/kg) was injected to a separate group of mice as a positive control. One-way Anova indicated significant effects (F=8.9, df=4.27, P<0.01). All doses had ‘anti-depressant’ effects, but only those of 0 and 30 mg/kg were significant. Data are presented as means±SEM. DMI=desipramine
*) P<0.05, **) P<0.01, ***) P<0.001 compared to vehicle
Posrsolt's Forced Swimming Test (FST)
Mice were placed in a 2 liter glass beaker (11 cm diameter) filled with water (24±1° C.) up to 30 cm from the bottom (so that the mouse could not touch the bottom and 8 cm from the rim (so that the mouse cannot escape). Immobility time (when the animal does not move except for small movements required to float) was recorded by 3 experimenters after 2, 6 and 9 min.
Cell Proliferation Test
Aliquots (200 μL) of suspensions of cancer cells were dispensed into wells of 96-well tissue culture plates at densities of 0.02×106 cells/well. Various concentrations of IA were introduced into the wells, and their efficacy was tested three days after initiation of the cultures, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The principle of this assay is that cells which survive following exposure to various compounds can reduce MTT to a dark -colored formazan, while dead cells are incapable of doing so. In each MTT assay every concentration of the cytotoxic substance was tested in five replicates in microplate wells. Assays with every cell line were carried out in two to three repeated experiments. The inhibitory effect of various compounds was calculated as percentage inhibition in comparison with the values obtained in untreated wells to which vehicle (ethanol 0.5%) was added.
For Example 15, the Following Experiments were Conducted:
Drug. IA was isolated as described above under Materials and Methods. It was then dissolved in ethanol for in vitro assays or in isopropanol for in vivo assays. A stock solution of 20 mg/ml for in-vitro assays and 50 mg/ml for in vivo assay was prepared.
Cell Culture
Human HEK 293 cells stably expressing TRPV1 were a kind gift from Merck Research Laboratories (Whitehouse Station, N.J.). Cells were cultured in minimal essential medium, Eagle, modified with non-essential amino acids, 1 mM sodium pyruvate, 2 mM L-glutamine and 1.5 g/L sodium bicarbonate (ATCC, Mabassas, Va.), containing 1% Penicillin-streptomycin, and 10% foetal bovine serum. Cells were passaged three times a week using Trypsin-EDTA 1× (Invitrogen, Carlsbad, Calif.) and grown under 5% CO2 at 37° C.
TRPV3-YFP [O'dell, D. K., Rimmerman, N., Pickens, S. R. & Walker J. M. Fatty acyl amides of endogenous tetrahydroisoquinolines are active at the recombinant human TRPV1 receptor. Bioorg. Med. Chem. 15, 6164-6169 (2007)], TRPV4 and mock-transfected cell lines were cultured in DMEM 1× with L-glutamine (Mediatech, Inc. Herndon, Va.), containing 1% penicillin-streptomycin (Invitrogen, Carlsbad, Calif.) and 10% foetal bovine serum.
HEK293 cells were transiently transfected with a rat TRPV2 plasmid using lipofectamine reagent (Invitrogen, Carlsbad, Calif.) according to manufacturer's protocol. They were then maintained in Dulbecco's modified Eagle Medium/10% fetal calf serum supplemented with Penicillin, Streptomycin, and L-glutamine. Primary keratinocytes from TRPV3-deficient and TRPV3+/+ mouse pups (day 1-4) were harvested and cultured as described previously [Chung, M. K., Lee, H., Mizuno, A., Suzuki, M. & Caterina, M. J. TRPV3 and TRPV4 mediate warmth-evoked currents in primary mouse keratinocytes. J. Biol. Chem. 279, 21569-21575 (2004)].
Calcium imaging of HEK 293 cells. TRPV1, TRPV3, and TRPV4 expressing HEK293 cells were plated 24-48 h before imaging in 96 well plates, loaded with 3 μM Fura-2 AM and imaged as previously described [O'dell, D. K., Rimmerman, N., Pickens, S. R. & Walker J. M. Fatty acyl amides of endogenous tetrahydroisoquinolines are active at the recombinant human TRPV1 receptor. Bioorg. Med. Chem. 15, 6164-6169 (2007)].
For single cell calcium imaging, HEK293-rat TRPV2 and HEK293-mouse TRPV3-YFP expressing cells were plated on collagen-coated glass cover slips. Cells were loaded for 60 min with 3 μM Fura-2 AM.
Calcium imaging of TRPV3+/+ and TRPV3−/− keratinocytes. Primary keratinocytes from TRPV3-deficient and WT mouse pups (day 1-4) were harvested and cultured as described [Chung, M. K., Lee, H., Mizuno, A., Suzuki, M. & Caterina, M. J. TRPV3 and TRPV4 mediate warmth-evoked currents in primary mouse keratinocytes. J. Biol. Chem. 279, 21569-21575 (2004)].
Cells were plated on glass coverslips (105/cm2) and incubated for 48-60 h, then loaded with fura-2 AM (20 μM, 0.04% pleuronic acid, 32° C. for 1 h) in imaging buffer containing (in mM): 130 NaCl, 2.5 CaCl2, 0.6 MgCl2, 10 HEPES, 1.2 NaHCO3, 10 glucose, pH 7.45. Ratiometric Ca2+ imaging was performed as previously described [O'dell, D. K., Rimmerman, N., Pickens, S. R. & Walker J. M. Fatty acyl amides of endogenous tetrahydroisoquinolines are active at the recombinant human TRPV1 receptor. Bioorg. Med. Chem. 15, 6164-6169 (2007)]. Drug was added to the bath following a period of baseline recording. Calcium measurements were made from 30 randomly selected cells per coverslip.
Electrophysiological recording. Currents were recorded using whole-cell voltage-clamp. Pipettes were pulled from microcapillary glass (A-M Systems). A coverslip containing cells was transferred to a 300 μL chamber that was constantly perfused (1-2 mL/min) with external solution. Voltage protocols were generated and data were digitized and recorded using Pulse (HEKA Elektronik) software in conjunction with an Axopatch 200A amplifier (Axon Instruments), and the data analyzed using an in-house Visual Basic (Microsoft) analysis program.
The pipette solution contained (in mM): 121.5 Kgluconate, 10 HEPES, 17 KCl, 9 NaCl, 1 MgCl2, 0.2 EGTA, 2 MgATP, and 0.5 NaATP, pH 7.2. The external solution contained (in mM): 120 NaCl, 5 KCl, 1 MgCl2, 2 CaCl2, 10 Glucose and 20 HEPES, pH 7.4 with NaOH. The measured charge (pC) was defined as the charge elicited between -85 and -45 mV by a ramping voltage stimulus (−85 mV to +35 mV, 0.54 mV/msec; holding potential -55 mV). Currents were sampled at 5 kHz. Experimental and control cells were alternated whenever possible. Control values were obtained from adjacent cells with no detectable YFP fluorescence, presumed to be non-TRPV3-expressing.
Data analysis of calcium imaging data. Analysis of calcium imaging data was done using a non-linear regression curve fit (Graphpad 4 Prism, San Diego, Calif.). t tests and one way ANOVA were calculated using SPSS (Chicago, Ill.). In the keratinocyte experiments, drug-induced response for each cell was taken as the maximal post-drug measurements over time minus the average of the last 5 pre-drug measurements. Averaged drug responses over 30 randomly selected cells per coverslip were analyzed with two-tailed unpaired t tests.
For analysis of c-Fos immunoreactivity, positive nuclei were identified based on their round form and optical density at least twice that of background. The numbers of c-Fos immunoreactive nuclei from the right and left hemispheres were averaged to obtain a representative number for the given region from each mouse. Student t tests were performed comparing the control (vehicle) with the IA group.
Responses to IA in WT versus TRPV3−/− mice were assessed using two-way analysis-of-variance (ANOVA) with Bonferroni post-hoc comparisons (Graphpad Prism 4 software).
Animals and procedures. Female Sabra mice (Harlan, Israel, 15-20 weeks old) and wild type C57BL/6 or TRPV3 KO female mice (18-20 weeks old) [Chung, M. K., Lee, H., Mizuno, A., Suzuki, M. & Caterina, M. J. TRPV3 and TRPV4 mediate warmth-evoked currents in primary mouse keratinocytes. J. Biol. Chem. 279, 21569-21575 (2004)] were used for behavioral assessments. 10 mice were housed in each cage. The animal care and protocols met the guidelines of the U.S. National Institutes of Health, detailed in the Guide for the Care and Use of Laboratory Animals, and were applied in conformity with the Institutional Ethics Committees. For the c-Fos immunostaining, female Sabra mice (see above) were used. Temperature in the animal room was maintained between 20-22° C., the light cycle was 12 h lights on (8:00-20:00 h); 12 h lights off (20:00-8:00 h). Mice were injected with intraperitoneal (i.p.) incensole acetate in a mixture of isopropanol:cremophor:saline (1:1:18) at a volume of 10 μl/g body weight.
IA and IN were assayed at different concentrations for their activity on IκBα degradation in TNFα-stimulated HeLa cells. Both compounds inhibited IκBα degradation in a dose dependent manner (
In order to demonstrate that IA inhibits the NF-κB pathway upstream from the IKKs experimentally, the effects of IA on TNFα-induced phosphorylation of the IKKs were tested. These experiments showed inhibition of IKKα/IKKβ phosphorylation by IA (
IA Blocks NF-κB-mediated Inflammatory Response in vitro and in vivo. To investigate whether the NF-κB inhibitory effect of IA confers an anti-inflammatory activity, it was determined, as detailed herein above in Materials and Methods, the levels of COX-2, nitric oxide production and ROS with and without IA in different cell lines. The in vivo anti-inflammatory activity of IA was examined in inflamed paw model in mice. COX-2 production in LPS-stimulated RAW 264.7 cells was inhibited by IA at a dose of 60 μM (P<0.001) (
Having established that IA inhibits the expression of several key inflammatory mediators in vitro, the anti-inflammatory properties of IA in vivo were studies. It was thereupon found that IA significantly reduced inflammation in the inflamed paw model in mice during a 4 hrs period. The decreased inflamed paw volume in the treated mice reflects a decrease in edema, which is a component of the inflammatory response. There were highly significant effects of treatment (F=11.7, df=3.64, P<0.001), time (F=10.6, df=4.64, P<0.0001) and interaction (F=3.9, df=12.64, P<0.001) (
To examine the effect of IA on functional recovery after CHI, the parameters of injured mice, treated with IA were compared with those of injured mice treated with vehicle.
At 1 h after CHI, the functional status of the mice was evaluated according to a set of 10 neurobehavioral tasks (neurological severity score, NSS) that tests reflexes, alertness coordination, and motor abilities. One point was awarded for absence of reflex or failure to perform a particular task. Hence, a score of 10 reflects maximal neurological impairment. Mice were equally divided to vehicle\ IA groups according to their NSS scores. Only mice with NSS>4 at 1 h after injury were included in the study. Immediately after NSS1h assessment, mice were randomly assigned to intraperitoneal (i.p.) injection with vehicle (isopropanol: Emulphor—a commercial emulsifier: saline=1:1:18) or with vehicle containing IA (50 mg/kg, n=9-10 mice/group). Recovery (ΔNSSt) was defined as the difference between NSS1h and NSS measured at any later time point and was determined at several time points up to 21 days following CHI.
NSS at 1 h were similar in both groups, (7.03±0.19 and 7.03±0.19, respectively) indicating no difference in the initial severity of injury. Markedly greater recovery of motor ability was observed in the IA group 24 h after injury as compared with vehicle (ΔNSS=1.00±0.12 vs 0.41±0.09, respectively, P=0.002) as depicted in
Memory function was assessed by ORT (Object Recognition Test) and the results are depicted in
A pronounced increase in tissue water content was observed in the left (ipsilateral) hemisphere of all injured mice at 24 h after injury, indicating the effect of injury in both groups. Although water accumulation tended to be smaller in IA mice (81.47±0.35% in IA vs 82.16±0.30% in vehicle) the difference did not reach statistical significance (P=0.15).
Since it was shown hereinabove that the pro-inflammatory cytokines TNF-α and IL-1β are upregulated within 1-4 h post-CHI, and that their inhibition is associated with better recovery. The mRNA levels of these cytokines were quantified at 3 hours after CHI using real-time PCR. Their amounts are expressed relative to β-actin, and it is apparent from
Thirty minutes after treatment with IA (namely, 90 min post CHI), a mild (˜1° C.) and short-term (˜30-60 min) duration of hypothermia was noted in IA—as compared to vehicle-treated mice (data is not shown).
When placed in an elevated plus-maze for the first time, a mouse's behavior is largely based on its anxiety level. Normal mice that have not received any anti-anxiety drugs will become moderately anxious in this new environment. Thus, they tend to prefer the closed arms over the less secure open aims. Meanwhile, mice treated with anti-anxiety drugs (e.g., diazepam, commonly known as valium) tend to be less anxious, so they spend more time in the 5 open arms compared to normal mice and they are generally less active. Forty five min after injection the mice were tested in the plus-maze for ‘anti-anxiety’ effects of IA (
*, P<0.05; **, P<0.01 compared to vehicle.
The c-Fos transcription factor is a product of an immediate early gene and its increase serves as a marker of enhanced neuronal activity. It is thus used in histological sections to map out brain regions that are activated or attenuated after treatment with psychoactive drugs. IA significantly increased c-Fos in the lateral septum, central nucleus of the amygdala and solitary nucleus, while significantly reducing c-Fos in the motor cortex, medial striatum and hippocampal CA3 region (
The data from the behavioral assays together with the c-Fos immunostaining establish the anxiolytic and anti-depressive effects of IA.
IA (75 mg/kg) exerted a potent anxiolytic-like effect in WT mice, while TRPV3−/− mice spent identical time on the open arms, regardless of whether they were injected IA or only vehicle (
These results indicate that the effects of IA in preclinical models for anti-depressants and anxiolytics are mediated via TRPV3 channels.
We used the Porsolt forced swimming test to examine the anti-depressant effect of IA. The method is based on the observation that a mouse, when forced to swim in a situation from which there is no escape, will, after an initial period of vigorous activity, eventually cease to move altogether making only those movements necessary to keep its head above water. This characteristic and readily identifiable behavioral immobility indicates a state of despair in which the rat has learned that escape is impossible and resigns itself to the experimental conditions. Fifty min after injection the mice were tested in the Porsolt forced swimming test for ‘anti-depressant’ effects (
*, P<0.05; **, P<0.01; ***, P<0.001 compared to vehicle
IA inhibited the proliferation of cells in several cell lines (
As cyto-toxic compounds often exhibit toxicity in the doses used for treatment, we examined IA for its general toxicity in mice (Sabra strain, both male and female and male Skid nod), both with high single doses (150 mg/kg) and with multiple doses (30 mg/kg* 3 times a week for a month). No weight loss was observed, nor any sign of toxicity or side effects.
To study the functional effects of IA on the CNS, IA was assayed in a panel of standard behavioral assays in mice (female Sabra strain, 15-20 weeks old), namely: the elevated plus maze [Crawley, J. N. What's Wrong with my Mouse? Behavioral Phenotyping of Transgenic and Knockout Mice (Wiley-Liss, New York, 2000)], the Porsolt forced-swimming test [Petit-Demouliere, B., Chenu, F. & Bourin, M. Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology 177, 245-255 (2005)], locomotion in the open field test and cataleptic response in a ring test [Fride, E. & Mechoulam, R. Pharmacological activity of the cannabinoid receptor agonist, anandamide, a brain constituent. Eur. J. Pharmacol. 231, 313-314 (1993). The elevated plus maze assay is based on the preference of mice for the closed arms of a maze, apparently due to fear of open spaces. At 50 mg/kg IA exerted a potent anxiolytic-like effect, causing mice to spend significantly more time in the aversive open arms of the maze. In the Porsolt forced-swim test, a standard assay for the evaluation of anti-depressant effects, IA significantly reduced the immobility recorded over 9 minutes, thus indicating a reversal of an avolition response. A significant reduction of open field behavior was observed, as well as impaired immobility on a ring. Dose dependency was noted in all assays (10-100 mg/kg, data not shown), and the findings were replicated in 7 independent experiments.
IA (100 μM) significantly increased calcium influx (EC50=16 μM; Hill slope=2.2;
IA also activated a cation current in moue TRPV3-YFP expressing HEK293 cells (
The effect of IA on different brain regions were studied by looking at the effect of IA on c-Fos immunoreactivity in mice brains 60 min after administration of IA (50 mg/kg; i.p.). The c-Fos transcription factor is a product of an immediate early gene and its increase serves as a marker of enhanced neuronal activity. It is thus used in histological sections to map out brain regions that are activated or attenuated after treatment with psychoactive drugs [Werme, M., Ringholm, A., Olson, L. & Brene S. Differential patterns of induction of NGFI-B, Norl and c-fos mRNAs in striatal subregions by haloperidol and clozapine. Brain Res. 863, 112-119 (2000); and Dragunow, M., Robertson, G. S., Faull, R. L., Robertson, H. A. & Jansen, K. D2 dopamine receptor antagonists induce fos and related proteins in rat striatal neurons. (1990) Neuroscience 37, 287-294]. IA significantly increased c-Fos in the lateral septum, central nucleus of the amygdala and solitary nucleus, while significantly reducing c-Fos in the motor cortex, medial striatum and hippocampal CA3 region (
The data from the behavioral assays together with the c-Fos immunostaining establish the anxiolytic and anti-depressive effects of IA. Given the robust effect of IA on TRPV3 channels and the observation that IA does not interact with a long list of receptors known to be involved in psychoactivity, the possibility that its behavioral effects are mediated through CNS TRPV3 channels was investigated. Thus, the panel of behavioral assays with WT and TRPV3−/− mice, which were administered either IA or vehicle was repeated.
IA (75 mg/kg) exerted a potent anxiolytic-like effect in WT mice, while TRPV3−/− mice spent identical time on the open arms, regardless of whether they were injected IA or only vehicle (
These results indicate that the effects of IA in preclinical models for anti-depressants and anxiolytics are mediated via TRPV3 channels.
Collectively, the data presented here, along with the expression of TRPV3 mRNA in the brain, indicate that TRPV3 channels affects emotional and behavioral processes in the CNS, in addition to its known effects on thermosensation.
All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/IL2007/001477 | 11/29/2007 | WO | 00 | 12/11/2009 |
| Number | Date | Country | |
|---|---|---|---|
| 60861441 | Nov 2006 | US | |
| 60956276 | Aug 2007 | US |
