Using the secondary structure (beta-sheet) of exosomal proteins for noninvasive pancreatic cancer detection

Information

  • Research Project
  • 10046870
  • ApplicationId
    10046870
  • Core Project Number
    R03CA252783
  • Full Project Number
    1R03CA252783-01
  • Serial Number
    252783
  • FOA Number
    PAR-18-021
  • Sub Project Id
  • Project Start Date
    7/7/2020 - 4 years ago
  • Project End Date
    6/30/2022 - 2 years ago
  • Program Officer Name
    BHARTI, SANITA
  • Budget Start Date
    7/7/2020 - 4 years ago
  • Budget End Date
    6/30/2022 - 2 years ago
  • Fiscal Year
    2020
  • Support Year
    01
  • Suffix
  • Award Notice Date
    7/7/2020 - 4 years ago

Using the secondary structure (beta-sheet) of exosomal proteins for noninvasive pancreatic cancer detection

PROJECT SUMMARY/ABSTRACT With a 5-year survival rate of less than 5%, pancreatic ductal adenocarcinoma (PDAC) as a devastating disease has an inferior prognosis, and prolonged survival is achieved only by resection with macroscopic tumor clearance. Although surgical resection can potentially cure early-stage disease, more than 80% of patients present with locally advanced or metastatic disease at the time of diagnosis and are ineligible for resection. Hence, early detection could significantly reduce mortality and improve prognosis. Exosomes secreted by tumor cells actively participate in tumor progression and metastasis and contain multiple biomolecules reflecting the status of their parental tumor cells. Although the biogenesis is still not clear, exosomes released into circulation are under intensive study mostly for its pivotal clinical potential for non- invasive early detection. Most of these studies focused on exosomal single protein/RNA/DNA, or molecular signatures based on multiplex assays. To date, none of the candidate markers have been validated to be adequate for clinical prognosis or diagnosis, which underlies the problem in current exosomal marker discovery approaches. We studied the collective attribute of exosomes by scrutinizing the secondary structure of the exosomal proteins. Our preliminary data strongly suggest that the tumor cell secretes more ?-sheet rich proteins through exosomes than the normal cell. The objective of this proposal is to validate the findings in multiple ways, providing evidence that the secondary structure of the exosomal proteins can be used for cancer detection. Successful data collection and analysis from this low-risk and high-reward study will not only justify that a tumor cell sheds more ?-sheet rich proteins through exosomes than a normal cell, but will also uncover an insight into the association between exosomes and pancreatic cancer cell states, thus providing a method that can potentially serve as biomarkers and therapeutic choices for pancreatic cancer.

IC Name
NATIONAL CANCER INSTITUTE
  • Activity
    R03
  • Administering IC
    CA
  • Application Type
    1
  • Direct Cost Amount
    100000
  • Indirect Cost Amount
    45000
  • Total Cost
    145000
  • Sub Project Total Cost
  • ARRA Funded
    False
  • CFDA Code
    394
  • Ed Inst. Type
    SCHOOLS OF ARTS AND SCIENCES
  • Funding ICs
    NCI:145000\
  • Funding Mechanism
    Non-SBIR/STTR RPGs
  • Study Section
    ZCA1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    NORTH DAKOTA STATE UNIVERSITY
  • Organization Department
    ENGINEERING (ALL TYPES)
  • Organization DUNS
    803882299
  • Organization City
    FARGO
  • Organization State
    ND
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    581086050
  • Organization District
    UNITED STATES