Claims
- 1. A vaccine composition suitable for administration to a canine, said composition comprising inactivated transmissible gastroenteritis virus of swine (TGEV).
- 2. A process for manufacturing an inactivated vaccine prepared from transmissible gastroenteritis virus of swine (a TGEV vaccine), which process comprises:
- (a) passing an isolate of TGE virus in cells of porcine and/or feline origin at a virus to cell ratio 2 of 1:1 to 1:10,000 to obtain an attenuated live TGE virus;
- (b) propagating the live TGE virus by cell culture in mammalian cells;
- (c) harvesting viral fluids so produced; and
- (d) inactivating the said viral fluids with a viral inactivating agent.
- 3. A process according to claim 2 in which the isolate of TGE virus is passed in cells of feline origin.
- 4. A process according to claim 2 in which the isolate of TGE virus is passed in Crandell kidney cells (ATCC CCL 94).
- 5. A process according to claim 2 in which the said viral fluids are inactivated with ascorbic acid and/or a salt thereof in the presence of oxygen and a source of heavy metal ions.
- 6. A process according to claim 5 in which the said viral fluids are inactivated with ascorbic acid and/or a salt thereof in the presence of oxygen and a source of heavy metal ions selected from the group consisting of salts of copper-, iron-, zinc-, and mercury-containing compounds, and compatible mixtures thereof.
Parent Case Info
This application is a division of application Ser. No. 07/677,620, filed Mar. 27, 1991, now U.S. Pat. No. 5,911,999, which is a continuation of application Ser. No. 07/243,252, filed Sep. 14, 1988 now abandoned.
Foreign Referenced Citations (1)
Number |
Date |
Country |
1058340 |
Feb 1967 |
GBX |
Non-Patent Literature Citations (4)
Entry |
Draghici et al. Lucr. Inst. Cercet. Vet. Bioperp. "Pasteur", 1978, vol. 14, pp. 31-37, abstract only. |
Murata, A., et al., Chem. Abs. 107:26455u (1987), and Agric. Biol. Chem, 51(3), 933-4 (1987). |
Woods, R.D., et al., Biological Abs., 82:53821 (1986) and Amer. J. Vet. Res., 47(6):1239-1242 (1986). |
Garwes, D.J., et al., Biological Abs., 72:14141 (1981) and J. Gen Virol., 52(1);153-158 (1981). |
Divisions (1)
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Number |
Date |
Country |
Parent |
677620 |
Mar 1991 |
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Continuations (1)
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Number |
Date |
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Parent |
243252 |
Sep 1988 |
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