Patent Application
20210220464
The present disclosure relates to a vaccine composition for preventing or treating an infectious disease caused by severe fever with thrombocytopenia syndrome (SFTS) virus.
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral disease that is endemic in China, Korea and Japan. No effective vaccine or specific treatment for SFTS is currently available. SFTS is a severe disease that causes symptoms such as high fever, vomiting, diarrhea, thrombopenia, leukopenia, and multiple organ failure, and has a mortality rate of 6 to 30% (Yu X J et al., N. Engl. J. Med. 2011; 364:1523-32; Ding F et al., Clin Infect Dis 2013: 56:1682-3).
In addition, seroconversion and viremia of SFTS virus have been found in domesticated animals such as goats, sheep, cattle, pigs and dogs, and these animals have been implicated as intermediate hosts in SFTS virus-endemic areas (Zhao L et al., Emerg Infect Dis 2013; 18: 963-5: Niu G et al., Emerg Infect Dis 2013; 19: 756-63).
Meanwhile, Chinese Patent Application Publication No. 102070704 discloses a kit for amplifying and detecting SFTS virus.
Accordingly, the present inventors have developed an effective vaccine against SFTS.
An object of the present disclosure is to provide a recombinant DNA or peptide of SFTS virus antigen that effectively induces an immune response in a subject, and an SFTS virus vaccine comprising the same.
Specifically, the present disclosure is intended to provide a vaccine composition for preventing or treating an infectious disease caused by severe fever with thrombocytopenia syndrome (SFTS) virus.
However, technical objects to be achieved by the present disclosure are not limited to the above-mentioned object, and other technical objects which are not mentioned herein will be clearly understood by those skilled in the art from the following description.
Hereinafter, various embodiments described herein will be described with reference to the drawings. In the following description, numerous specific details are set forth, such as specific configurations, compositions, and processes, etc., in order to provide a thorough understanding of the present disclosure. However, certain embodiments may be practiced without one or more of these specific details, or in combination with other known methods and configurations. In other instances, known processes and preparation techniques have not been described in particular detail in order to not unnecessarily obscure the present disclosure. Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, configuration, composition, or characteristic described in connection with the embodiment is included in at least one embodiment of the present disclosure. Thus, the appearances of the phrase “in one embodiment” or “an embodiment” in various places throughout this specification do not necessarily refer to the same embodiment of the present disclosure. Additionally, the particular features, configurations, compositions, or characteristics may be combined in any suitable manner in one or more embodiments.
Unless otherwise stated in the specification, all the scientific and technical tenns used in the specification have the same meanings as commonly understood by those skilled in the technical field to which the present disclosure pertains.
As used herein, the term “preventing” refer to any action of delaying viral growth, proliferation, invasion or infection by administering the composition of the present disclosure.
As used herein, the terms “treating” and “alleviating” refer to any action of alleviating or beneficially changing SFTSV-related diseases by suppressing viral growth, proliferation or infection through administration of the composition of the present disclosure.
The present disclosure relates to a virus vaccine comprising a recombinant DNA or peptide of virus antigen and a vaccination method using the same, and also to improvement in a vaccination method comprising a step of introducing into a mammalian body a nucleotide sequence encoding an immunogen which is an antigenic protein or peptide (where the protein or peptide is expressed in the mammalian body, causing an immune response against the antigenic protein or peptide). This vaccination method is known.
According to one embodiment of the present disclosure, there is provided an antigenic composition comprising, as an active ingredient, any one or more recombinant peptides selected from the group consisting of:
a first recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 287, or which is encoded by a first recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 286:
a second recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 289, or which is encoded by a second recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 288;
a third recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 291, or which is encoded by a third recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 290:
a fourth recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 293, or which is encoded by a fourth recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 292; and
a fifth recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 295, or which is encoded by a fifth recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 294.
The antigenic composition of the present disclosure may comprise at least one of: the first recombinant peptide which comprises the amino acid sequence represented by SEQ ID NO: 287, or which is encoded by the first recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 286; and the second recombinant peptide which comprises the amino acid sequence represented by SEQ ID NO: 289, or which is encoded by the second recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 288.
The antigenic composition of the present disclosure may comprise: the first recombinant peptide which comprises the amino acid sequence represented by SEQ ID NO: 287, or which is encoded by the first recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 286; and the second recombinant peptide which comprises the amino acid sequence represented by SEQ ID NO: 289, or which is encoded by the second recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 288.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 1 to 76.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 1 to 38.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 39 to 76.
The antigenic composition of the present disclosure may comprise: a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 1 to 38; and a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 39 to 76.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 77 to 152.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 77 to 114.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 115 to 152.
The antigenic composition of the present disclosure may comprise: a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 77 to 114; and a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 115 to 152.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 153 to 186.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 187 to 227.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 187 to 207.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 208 to 227.
The antigenic composition of the present disclosure may comprise: a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 187 to 207; and a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 208 to 227.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 228 to 285.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 228 to 256.
The antigenic composition of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 257 to 285.
The antigenic composition of the present disclosure may comprise: a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 228 to 256; and a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 257 to 285.
In the present disclosure, the antigenic composition is injected in vivo through a route selected from among intramuscular, intradermal, subcutaneous, subepidermal, transdermal and intravenous routes, but the route is not limited thereto. In the present disclosure, the antigenic composition is injected into a subject through intramuscular injection. In the present disclosure, the antigenic composition is injected into a subject through intradermal injection. In the present disclosure, the in vivo injection of the antigenic composition into the subject is followed by electroporation.
The antigenic composition of the present disclosure may further comprise an adjuvant. In the present disclosure, the adjuvant may be at least one of IL-7 and IL-33, preferably IL-33, but is not limited thereto.
According to another embodiment of the present disclosure, there is provided a vaccine comprising, as an active ingredient, any one or more recombinant peptides selected from the group consisting of:
a first recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 287, or which is encoded by a first recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 286:
a second recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 289, or which is encoded by a second recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 288:
a third recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 291, or which is encoded by a third recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 290:
a fourth recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 293, or which is encoded by a fourth recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 292; and
a fifth recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 295, or which is encoded by a fifth recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 294:
The vaccine of the present disclosure may comprise at least one of: the first recombinant peptide which comprises the amino acid sequence represented by SEQ ID NO: 287, or which is encoded by the first recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 286; and the second recombinant peptide which comprises the amino acid sequence represented by SEQ ID NO: 289, or which is encoded by the second recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 288.
The vaccine of the present disclosure may comprise at least one of: the first recombinant peptide which comprises the amino acid sequence represented by SEQ ID NO: 287, or which is encoded by the first recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 286; and the second recombinant peptide which comprises the amino acid sequence represented by SEQ ID NO: 289, or which is encoded by the second recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 288.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 1 to 76.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 1 to 38.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 39 to 76.
The vaccine of the present disclosure may comprise: a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 1 to 38; and a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 39 to 76.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 77 to 152.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 77 to 114.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 115 to 152.
The vaccine of the present disclosure may comprise: a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 77 to 114; and a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 115 to 152.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 153 to 186.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 187 to 227.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 187 to 207.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 208 to 227.
The vaccine of the present disclosure may comprise: a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 187 to 207; and a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 208 to 227.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 228 to 285.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 228 to 256.
The vaccine of the present disclosure may comprise a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 257 to 285.
The vaccine of the present disclosure may comprise: a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 228 to 256; and a peptide represented by the amino acid sequence of at least one of SEQ ID NOs: 257 to 285.
In the present disclosure, the vaccine is injected in vivo through a route selected from among intramuscular, intradermal, subcutaneous, subepidermal, transdermal and intravenous routes, but the route is not limited thereto. In the present disclosure, the vaccine is injected into a subject through intramuscular injection. In the present disclosure, the vaccine is injected into a subject through intradermal injection. In the present disclosure, the in vivo injection of the vaccine into the subject is followed by electroporation.
In the present disclosure, the vaccine may further comprise an adjuvant. In the present disclosure, the adjuvant may be at least one of IL-7 (SEQ ID NO: 296) and IL-33 (SEQ ID NO: 297), preferably IL-33, but is not limited thereto.
The antigenic composition or vaccine of the present disclosure may further comprise a solvent, an excipient, and the like. Examples of the solvent include, but are not limited, saline and distilled water, and examples of the excipient include, but are not limited to, aluminum phosphate, aluminum hydroxide, and aluminum potassium sulfate. In addition, the antigenic composition or vaccine of the present disclosure may further comprise substances that are commonly used for vaccine production in the art to which the present disclosure pertains.
The antigenic composition or vaccine of the present disclosure may be produced by methods that are commonly used in the art to which the present disclosure pertains. The antigenic composition or vaccine of the present disclosure may be prepared as an oral or parenteral formulation, and is preferably prepared as an injectable liquid formulation which is a parenteral formulation. The antigenic composition or vaccine of the present disclosure may be administered through an intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, nasal or epidural route.
The antigenic composition or vaccine of the present disclosure may be administered to a subject in an immunologically effective amount. The term “immunologically effective amount” refers to an amount sufficient to exhibit the effect of preventing or treating severe fever with thrombocytopenia syndrome (SFTS) or SFTS virus infection, and an amount that does not cause side effects or serious or excessive immune responses. The exact dose of the antigenic composition or vaccine of the present disclosure may vary depending on the specific immunogen to be administered, and may be easily determined by those skilled in the art depending on factors well known in the medical field, including the age, body weight, health and sex of a subject to be prevented or treated, the drug sensitivity of the subject, the route of administration, and the mode of administration. The antigenic composition or vaccine of the present disclosure may be administered once or several times.
The vaccine of the present disclosure is administered in a pharmaceutically effective amount. The term “pharmaceutically effective amount” refers to an amount sufficient to exhibit a vaccination effect, and an amount that does not cause side effects or serious or excessive immune responses. The exact dose of the vaccine may vary depending on the antigen to be administered, and may be easily determined by those skilled in the art depending on factors well known in the medical field, including the age, body weight, health and sex of a subject, the drug sensitivity of the subject, the route of administration, and the mode of administration. The vaccine may be administered once or several times.
The DNA vaccine of the present disclosure is a DNA vaccine in which a nucleotide encoding the epitope peptide of the present disclosure is contained in a pharmaceutically acceptable carrier. The DNA vaccine is preferably in the form of a DNA plasmid, most preferably in the form of a mock plasmid (derived from pVax-1), but is not limited thereto. Therefore, it is preferable that the above-described nucleotides are inserted into various known recombinant expression vectors.
According to still another embodiment of the present disclosure, there is provided an expression vector comprising any one or more recombinant DNAs selected from the group consisting of:
a first recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 286;
a second recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 288;
a third recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 290:
a fourth recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 292; and
a fifth recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 294.
The expression vector of the present disclosure may comprise at least one recombinant DNA selected from among: the first recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 286; and the second recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 288.
The expression vector of the present disclosure may comprise: the first recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 286: and the second recombinant DNA comprising the nucleotide sequence represented by SEQ ID NO: 288.
As used herein the term “vector” refers to a means for expressing a target gene in a host cell. The vector may comprise elements for expression of the target gene, including a replication origin, a promoter, an operator gene, and a terminator sequence, and may further comprise appropriate enzyme sites (e.g., restriction enzyme sites) for introduction into the genome of the host cell, and/or selection markers for confirming successful introduction into the host cell, and/or a ribosome binding site (RBS) for translation into a protein, internal ribosome entry site (IRES), and the like. The vector may be engineered by a conventional genetic engineering method so as to have a fusion polynucleotide (fusion promoter) as a promoter. The vector may further comprise a transcription control sequence (e.g., an enhancer, etc.), in addition to the promotor.
As used herein, the term “expression vector” refers to a recombinant vector capable of expressing a target peptide in a host cell of interest, and refers to a gene construct comprising essential regulatory elements operatively linked to express a gene insert. The expression vector comprises expression regulatory elements such as an initiation codon, a stop codon, a promoter and an operator. The initiation and stop codons are generally considered to be part of the nucleotide sequence encoding the polypeptide, and must exhibit an action in a subject when the gene construct has been administered and must be in frame with the coding sequence. The promoter of the vector may be constitutive or inducible. The vector may be introduced into a host cell in the form of an expression cassette, which is a gene construct including all elements necessary for self-expression. The expression cassette may include a promoter operatively linked to a gene insert to be expressed, a transcription termination signal, a ribosome binding site, and a translation termination signal. The expression cassette may be in the form of a self-replicable expression vector. In the present disclosure, the expression vector may be a viral or non-viral vector. The viral vector may be an adenovirus vector, a retrovirus vector including lentivirus, an adeno-associated virus vector, or a herpes simplex virus vector, but is not limited thereto. In addition, the non-viral vector may be a plasmid vector, a bacteriophage vector, a liposome, a bacterial artificial chromosome, or a yeast artificial chromosome, but is not limited thereto.
In the present disclosure, the target gene in the expression vector may be operatively linked to the fusion polynucleotide. The term “operatively linked” refers to a functional linkage between a gene expression regulatory sequence and another nucleotide sequence. The gene expression regulatory sequence may regulate transcription and/or translation of other nucleotide sequences by being “operatively linked”. In the expression vector, in order for the fusion polynucleotide to be operatively linked to the target gene, the fusion polynucleotide may be linked to the 5′ end of the target gene. The expression vector of the present disclosure may be used as a target protein expression vector capable of expressing the target protein with high efficiency in an appropriate host cell when the gene encoding the target protein to be expressed is operatively linked.
The expression vector of the present disclosure may further comprise, as an adjuvant, a gene encoding at least one of IL-7 (SEQ ID NO: 298) and IL-33 (SEQ ID NO: 299), preferably a gene encoding IL-33.
The expression vector of the present disclosure may further comprise a transcription regulatory sequence. The transcription regulatory sequence may be at least one selected from the group consisting of: a transcription termination sequence such as a polyadenylation sequence (pA); a replication origin such as an fl replication origin, an SV40 replication origin, a pMB1 replication origin, an adeno replication origin, an AAV replication origin, or a BBV replication origin; a Kozak sequence (AACAATGGC), which is known to be highly likely to increase gene expression by increasing the recognition rate of ribosomes at the initiation point (ATG) of the translation process; and an IgE leader sequence, but is not limited thereto.
The expression vector of the present disclosure may further comprise a restriction enzyme cleavage site. The restriction enzyme cleavage site refers to a specific nucleotide sequence that is specifically recognized and cleaved by a restriction enzyme. The cleavage sites may be sequences that are specifically recognized by restriction enzymes such as EcoRI, BamHI, HindIII, KpnI, SalI, NotI, NcoI, PstI, SmaI and XhoI.
In addition, the expression vector in the present disclosure may further include a selection marker. The selection marker is a gene for confirming whether the expression vector has been successfully introduced into a host cell or for constructing a stable cell line, and may be at least one selected from selected from the group consisting of, for example, genes resistant to drugs such as antibiotics, metabolism-related genes, and genes for gene amplification.
The expression vector of the present disclosure may include an IL-7-encoding gene (SEQ ID NO: 298) together with the recombinant DNA. In this case, the expression vector (preferably an expression plasmid) may comprise, as a restriction enzyme cleavage site, any one or more selected from the group consisting of BamHI, NcoI, and NotI. As an example, the expression vector may include a nucleotide sequence represented by SEQ ID NO: 300 together with the recombinant DNA.
The expression vector of the present disclosure may include an IL-33-encoding gene (SEQ ID NO: 299) together with the recombinant DNA. In this case, the expression vector (preferably an expression plasmid) may include, as a restriction enzyme cleavage site, any one or more selected from the group consisting of BamHI, NcoI and NotI. As an example, the expression vector may include a nucleotide sequence represented by SEQ ID NO: 301 together with the recombinant DNA.
In the present disclosure, the expression vector may be expressed in a host cell. For example, the host cell may strongly induce transcription initiation in animal cells. Specifically, the host cell may induce transcription initiation in mammalian cells, for example, animal cells such as human cells.
The expression vector of the present disclosure may be constructed by various methods known in the art.
According to yet another embodiment of the present disclosure, there is provided a transformant obtained by introducing the expression vector provided according to the present disclosure into a host cell by transformation.
In the present disclosure, transfer (introduction) of the expression vector into a cell may be performed using a transfer method well known in the art. Examples of the transfer method include, but are not limited to, microinjection, calcium phosphate precipitation, electroporation, sonoporation, magnetofection, liposome-mediated transfection, gene bombardment, and a method using dendrimers and inorganic nanoparticles.
The transfonant may be produced by transforming the above-described expression vector into a cell.
As used herein, the term “transformant” refers to a cell or plant transformed by a DNA construct comprising a DNA sequence which is operatively linked to a promoter and encodes a useful substance. In the present disclosure, the transformant is meant to include a transformed microorganism animal cell or plant cell, a transformed animal or plant, and a cultured cell derived therefrom.
According to still yet another embodiment of the present disclosure, there is provided a method for preventing or treating severe fever with thrombocytopenia syndrome (SFTS) virus infection, the method comprising a step of administering to a subject an effective amount of the above-described antigenic composition, the above-described vaccine, the above-described expression vector, or the above-described transformant.
According to a further embodiment of the present disclosure, there is provided a pharmaceutical composition for preventing or treating severe fever with thrombocytopenia syndrome (SFTS) virus infection, the pharmaceutical composition comprising, as an active ingredient, the above-described antigenic composition, the above-described vaccine, the above-described expression vector, or the above-described transformant.
In the present disclosure, the pharmaceutical composition may be in the form of capsules, tablets, granules, or injections, ointments, powders, or beverages, and the pharmaceutical composition may be for administration to humans.
For use, the pharmaceutical composition of the present disclosure may be formulated in the form of each of oral preparations, including powders, granules, capsules, tablets or aqueous suspensions, skin external preparations, suppositories, and sterile injectable solutions, according to conventional methods, but is not limited thereto. The pharmaceutical composition of the present disclosure may contain a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers that may be used in the present disclosure include: binders, lubricants, disintegrants, excipients, solubilizers, dispersing agents, stabilizers, suspending agents, colorants, fragrances and the like, which may be used for oral administration: buffers, preservatives, pain-relieving agents, solubilizers, isotonic agents, stabilizers and the like, which may be used for injection: and bases, excipients, lubricants, preservatives and the like, which may be used for local administration. The pharmaceutical composition of the present disclosure may be formulated in various ways by mixing it with the pharmaceutically acceptable carrier as described above. For example, for oral administration, the pharmaceutical composition of the present disclosure may be formulated in the form of tablets, troches, capsules, elixirs, suspensions, syrups, wafers or the like, and for injection, may be formulated in the form of unit dose ampoules or multi-dose vials. In addition, the pharmaceutical composition of the present disclosure may be formulated as solutions, suspensions, tablets, capsules, sustained-release preparations, or the like.
Meanwhile, examples of carriers, excipients and diluents suitable for formulation include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxy benzoate, propylhydroxy benzoate, talc, magnesium stearate, and mineral oil. In addition, the pharmaceutical composition of the present disclosure may further contain a filler, an anticoagulant, a lubricant, a wetting agent, a fragrance, an emulsifier, a preservative or the like.
The routes of administration of the pharmaceutical composition according to the present disclosure include, but are not limited to, oral, intravenous, intramuscular, intra-arterial, intramedullary, intradural, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, gastrointestinal, topical, sublingual and intrarectal routes. Oral or parenteral administration is preferred.
As used herein, the term “parenteral” is meant to include subcutaneous, transdermal, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intradural, intra-lesional and intra-cranial injection or infusion techniques. The pharmaceutical composition of the present disclosure may also be formulated as suppositories for intrarectal administration.
The pharmaceutical composition of the present disclosure may vary depending on various factors, including the activity of a specific compound used, the patient's age, body weight, general health, sex and diet, the duration of administration, the route of administration, excretion rate, the drug content, and the severity of a specific disease to be prevented or treated. The dose of the pharmaceutical composition may vary depending on the patient's condition, body weight, the severity of the disease, the form of drug, and the route and period of administration, but may be suitably selected by a person skilled in the art and may be 0.0001 to 50 mg/kg/day or 0.001 to 50 mg/kg/day. The pharmaceutical composition may be administered once or several times a day. The dose is not intended to limit the scope of the present disclosure in any way. The pharmaceutical composition according to the present disclosure may be formulated as pills, sugar-coated tablets, capsules, liquids, gels, syrups, slurries, or suspensions.
A recombinant DNA or peptide of SFTS virus and an SFTS virus vaccine comprising the same, which are provided according to the present disclosure, exhibit an excellent effect on the prevention and treatment of SFTS virus infection by effectively inducing an immune response to SFTS virus in a subject.
According to one embodiment of the present disclosure, there is provided an antigenic composition or vaccine comprising, as an active ingredient, any one or more recombinant peptides selected from the group consisting of: a first recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 287, or which is encoded by a first recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 286; a second recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 289, or which is encoded by a second recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 288; a third recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 291, or which is encoded by a third recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 290; a fourth recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 293, or which is encoded by a fourth recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 292: and a fifth recombinant peptide which comprises an amino acid sequence represented by SEQ ID NO: 295, or which is encoded by a fifth recombinant DNA comprising a nucleotide sequence represented by SEQ ID NO: 294.
The antigenic composition or vaccine of the present disclosure may further comprise an adjuvant. In this case, the adjuvant may be at least one of IL-7 and IL-33. preferably IL-33, but is not limited thereto.
Mode for Invention Hereinafter, the present disclosure will be described in detail with reference to examples. However, the following examples serve merely to illustrate the present disclosure, and the scope of the present disclosure is not limited by the following examples.
Synthesis of Five SFTS Virus Antigen Genes and Two Adjuvant Genes
IgE leader and Kozak sequences were inserted into the 5′ end of each target gene (SFTSV antigen or adjuvant gene), and a termination codon was inserted into the 3′ end of the target gene. Finally, restriction enzyme sequences (5′ BamHI and 3′ Not) were inserted into both ends of the gene, followed by gene synthesis.
Cloning into High-Efficiency Backbone Plasmid (pGX0001)
After completion of synthesis of each insertion gene, each insertion gene was cleaved with BamHI and NotI and inserted into a high-efficiency backbone plasmid (pGX0001) cleaved with the same restriction enzymes, thereby constructing candidate plasmids (see
Optimization of Sequence of DNA Vaccine Highly Expressing Antigen In Vivo
As five optimal antigens. Gc (glycoprotein C). Gn (glycoprotein N). NP (nucleocapsid protein), NS (non-structural protein) and RdRp (RNA dependent RNA polymerase) were selected, and as two optimal adjuvants, IL-7 and IL-33 were selected. For the five antigens, a consensus sequence derived from 27 to 32 SFTS virus strains isolated from Korean. Chinese and Japanese was secured. The consensus sequence was designed as a universal antigen sequence with cross-immunity using the amino acid sequence of an antigen common to various SFTS virus subtypes and variants. Major human MHC class I and II epitopes present in various SFTS virus subtypes and variants were identified by using in silico immunoinformatics techniques, and antigen sequences were designed to contain these epitopes. Thereafter, based on the optimized amino acid sequences of the SFTS virus antigens, nucleotide sequences for DNA vaccines were finally derived.
Expression cassette structures according to one embodiment of the present disclosure, which are used in the following experiment, were configured to contain a high-expression promoter (plasmid backbone sequence), a Kozak sequence, an IgE leader sequence, and a poly-A signal sequence (plasmid backbone sequence). At this time, the Kozak and IgE leader sequences were inserted upstream of the target gene (SFTSV antigen or adjuvant) in order to increase the expression level of the gene in vivo. Meanwhile, in order to increase the expression level of the antigen gene in vivo, the sequences of five SFTS virus antigens (Gc, Gn, NP, NS, and RdRp) were optimized with human codons.
Generation of DNA Vaccine Lead Candidates Expressing SFTS Virus Antigen
An IgE leader sequence and a Kozak sequence were inserted into 5′ end of each target gene (SFTSV antigen or adjuvant gene), and a stop codon was inserted into the 3′ end thereof. Finally, restriction enzyme sequences (5′ BamHI and 3′ NotI) were inserted into both ends of each gene, followed by gene synthesis. After completion of synthesis of each insertion gene, each insertion gene was cleaved with BamHI and NotI and inserted into a high-efficiency backbone plasmid (pGX0001) cleaved with the same restriction enzymes, thereby constructing candidate plasmids. The results of gene synthesis and cloning were confirmed by gene sequencing.
Evaluation of Immunogenicity of SFTS Virus Antigen-Expressing DNA Vaccines Using Mouse Models
Overlapping peptide (OLP) pools for immunogenicity evaluation were created.
Specifically, in order to evaluate the immunogenicity of the five SFTS virus antigens, the sequence of each antigen was fragmented into 15-mer peptides overlapping 8 amino acids. The purity of each of the peptides was qualitatively and quantitatively analyzed using high performance liquid chromatography and electrospray mass spectrometry in the production process. Through this process, a total of 76 peptides were obtained from the Gn antigen, and 38 of these peptides were mixed together to prepare OLP1 and OLP2 (25 μg/ml each peptide) for Gn (Table 1). From the Gc antigen, a total of 76 peptides were obtained and 38 of these peptides were mixed together to prepare OLP3 and OLP4 (Table 2). From the NP antigen, a total of 34 peptides were obtained and mixed together to prepare OLP5 (Table 3). From the NS antigen, a total of 41 peptides were obtained and mixed together to prepare OLP6 (Table 4). From the RdRp antigen, a total of 58 peptides were obtained, and 29 of these peptides mixed together to prepare OLP7 and OLP8 (Table 5).
More specifically, as shown in Tables 1 to 5 below, the mixture of SEQ ID NO: 1 to SEQ ID NO: 38 is OLP1. In addition, the mixture of SEQ ID NO: 39 to SEQ ID NO: 76 is OLP2. The mixture of SEQ ID NO: 77 to SEQ ID NO: 114 is OLP3. The mixture of SEQ ID NO: 115 to SEQ ID NO: 152 is OLP4. The mixture of SEQ ID NO: 153 to SEQ ID NO: 186 is OLP5. The mixture of SEQ ID NO: 187 to SEQ ID NO: 227 is OLP6. The mixture of SEQ ID NO: 228 to SEQ ID NO: 256 is OLP7. The mixture of SEQ ID NO: 257 to SEQ ID NO: 285 is OLP8.
The OLP pools created as described above were used for evaluation of T-cell immune responses in the following experiment.
Validation of Immunogenicity of Five SFTS Virus Antigens and Two Adjuvants
For vaccination. BALB/c mice were divided into the following five groups, each consisting of 6 mice: a naïve group; a group injected intramuscularly with a DNA vaccine: a group injected intramuscularly with a DNA vaccine and then subjected to electroporation: a group injected intramuscularly with a DNA vaccine and an IL-7 adjuvant and then subjected to electroporation; and a group injected intramuscularly with a DNA vaccine and an IL-33 adjuvant and then subjected to electroporation. Each mouse of the naïve group was vaccinated with 200 μg of a plasmid into which no SFTS virus gene was inserted, and each mouse of each of the other four groups was vaccinated with a total of 200 μg (40 μg for each DNA) of five SFTS virus antigen-expressing DNAs (DNA sequences of Gn, Gc, NP, NS and RdRp, which correspond to the DNA sequences of SEQ ID NOs: 286, 288, 290, 292 and 294, respectively, and to the amino acid sequences of SEQ ID NOs: 287, 289, 291, 293 and 295, respectively). In addition, each mouse of the groups to be vaccinated with IL-7 and IL-33 adjuvants was vaccinated with 50 μg of each adjuvant in addition to each DNA. For the mice of three groups, except the naïve group and the group injected intramuscularly with the DNA vaccine, the vaccination site of each mouse was subjected to electroporation using an electroporator at 0.2 A immediately after intramuscular injection. 21 days after the first vaccination, the second vaccination was performed using the same amount of the DNA. 21 days after the second vaccination, the mice were sacrificed, and the spleens and inguinal lymph nodes were isolated and used for immunogenicity evaluation.
Validation of T-Cell Immune Response to SFTSV Vaccine Candidate
ELISpot assay was performed to measure T-cell response specific to SFTS virus (
Evaluation of Multifunctionality of T Cells Induced by SFTSV DNA Vaccine Candidate
The spleen cells isolated from the vaccinated mice were stimulated with each of the SFTS virus OLPs. and then analyzed by intracellular cytokine staining (ICS) using multicolor.
The cells were stimulated with each of the eight SFTS virus OLPs shown in Tables to 5 above, and were sorted into T-cell subsets secreting IFN-γ, TNF-α and IL-2, respectively. The proportion of T-cells secreting each cytokine was determined and the results are shown in
As shown in
It was confirmed that OLP6, an OLP corresponding to the NS protein of SFTS virus, induced the strongest immune response, and in particular, a very strong immune response appeared in cells secreting IFN-γ and TNF-α. In addition, it could be very clearly confirmed in the CD8+ T cells treated with OLP6 that the IMEP group showed a stronger immune response than the IM group, and that the strongest immune response was induced when IL-33 was used as an adjuvant.
This means that, when electroporation and IL-33 are used, the proportion of SFTS virus-specific T-cells induced by the vaccine is further increased.
Analysis of Multifunctionality of T-Cells Induced by Vaccine Candidate
Based on FACS data, the multifunctionality of T-cells in each group was analyzed. Based on the results of FACS, the multifunctionality of CD8+ T cells stimulated with OLP6 inducing the strongest immune response was summarized for each group and the results were recorded. The results indicated that the proportion of mulifunctional T cells was higher in the IMEP group than in the IM group and was the highest in the group injected with IL-33 as an adjuvant in addition to being subjected to electroporation. This means that the T-cell immune response induced by the vaccine was qualitatively better when electroporation and IL-33 are used. This tendency also appeared in the proportion of multifunctional T cells among total effector T cells (
Evaluation of Antibody Formation Ability of SFTSV Vaccine Candidate
Verification of SFTSV-Specific Antibody Production Response Induced by Vaccine
Enzyme-linked immunosorbent assay (ELISA) was performed to measure an SFTS virus-specific antibody production response induced by SFTSV vaccine. In order to establish an ELISA technique for the recombinant SFTSV NP antigen protein, an experiment was conducted using the serum of mice evaluated to have vaccine-induced immunogenicity. Using the ELISA assay technique as described above, the antibody immune response induced by the vaccine was quantitatively analyzed. As shown in
Quantitative Evaluation of Neutralizing Antibody Titer Induced by SFTSV DNA Vaccine
The neutralizing antibody titers of 33 antibodies produced in the mice by the DNA vaccine candidates and various adjuvants were measured by PRNT50 assay.
The mouse standard antibody developed by the present inventors was used as a positive control. The experimental results indicated that the animals of the test groups showed an SN titer of 20 to 160, suggesting that a neutralizing antibody was formed. In particular, a stronger neutralizing antibody response was observed in the group (IMEP) injected with the DNA vaccine by intramuscular injection+electroporation, and a very weak neutralizing antibody titer was detected in the microneedle group (Microneedle) (
Establishment of Medium-Sized Animal Models as SFTSV-Infected Animal Models
New animal models for validating the infection inhibitory effect of the SFTSV vaccine were developed. As a result of infecting medium-sized animals with SFTSV isolated from an SFTS patient, it was observed that the virus was detected in the blood and that the platelet count continued to decrease up to day 8 after infection (
Validation of Immunogenicity of SFTSV Vaccine Using SFTSV-Infected Animal Models
The present inventors used the established SFTSV-infected medium-sized animal models as models to validate the infection inhibitory effect of the SFTSV vaccine. Each animal of a vaccination group (N=6) was vaccinated with a total of 1 mg (200 μg for each DNA) of the five SFTS virus antigen-expressing DNAs (see Table 6 below) by intradermally injecting the DNAs into both femurs in an amount of 500 μg for each femur. Each animal of a control group was vaccinated with 1 mg of a mock plasmid (derived from pVax-1), into which no SFTS virus gene was inserted, by intradermally injecting the DNAs into both femurs in an amount of 500 μg for each femur. Both the two groups were subjected to electrophoresis using an electroporator at 0.2 A immediately after intradermal injection. The vaccination was performed a total of 5 times, once every two weeks (vaccinated on days 0, 14, 28, 42 and 56).
Evaluation of T-Cell Immune Response Induced by Vaccine in SFTSV-Infected Medium-Sized Animal Models
Before vaccination and 2 weeks after each of 2nd vaccination, 4th vaccination and 5th vaccination, 5 ml of blood was sampled, PBMCs and serum were isolated therefrom, and SFTSV-specific T cell immune response (ELISpot assay) and antibody immune response (ELISA and neutralizing antibody assay) were measured. SFTSV-specific T cell immune response induced by the vaccine was evaluated by ELISpot assay. As shown in
Quantitative Evaluation of Antibody Immune Response and Neutralizing Antibody Titer Induced by Vaccine in SFTSV-Infected Medium-Sized Animal Models
The formation of SFTSV-specific reactive antibody by the DNA vaccine was measured and evaluated by ELISA assay. As shown in
The neutralizing antibody titer of the antibody induced by the DNA vaccine was measured by PRNT50 assay. As shown in
Evaluation of Preventive Effect of SFTSV Preventive DNA Vaccine in SFTSV-Infected Medium-Sized Animal Models
To evaluate the preventive effect of the SFTSV vaccine the animals vaccinated with SFTSV vaccine were infected with a lethal dose of SFTSV, and then evaluated for clinical symptoms such as survival rate, SFTSV viral load, platelet count, and body temperature and body weight changes. As shown in
SFTSV viral load was measured by real-time PCR. As shown in
It was observed that this animal was the same animal as the animal whose platelets have increased, and as the viral load therein decreased, the platelet count thereof was also returned to normal.
As a result of counting platelets, it was observed that the platelet count of the control group was decreased rapidly by SFTSV infection (
Meanwhile, as shown in
Taking these results together, it could be seen that the SFTSV-preventive DNA vaccine candidate developed by the present inventors could effectively prevent SFTSV infection, as confirmed through verification of various clinical indicators (survival rate, platelet count, body temperature, and body weight) in the medium-sized animal models.
Evaluation of Cross-Reactivity of Neutralizing Antibody Induced in Mice and Medium-Sized Animals
To evaluate the cross-reactivity of SFTSV neutralizing antibody produced in mice after SFTSV DNA vaccination, a PRNT50 test was performed using other SFTS viruses. As shown in
In addition, to evaluate the cross-reactivity of SFTSV neutralizing antibody produced in medium-sized animals after SFTSV DNA vaccination, a PRNT50 test was performed using other SFTS viruses. As shown in
Evaluation of Immunogenicity of SFTSV Preventive DNA Vaccine in SFTSV-Infected Medium-Sized Animal Models
Using the SFTSV-infected medium-sized animal models established as described above, the infection preventive effect of the SFTSV vaccine was evaluated. To evaluate the preventive effect of each vaccine, SFTSV-vaccinated groups (N=4, N=3, N=3, and N=3, respectively) were vaccinated respectively with 1 mg of a Gn/Gc vaccine, an NP vaccine, an NS vaccine and an RdRp vaccine, which are SFTS virus antigen-expressing DNAs, by intradermally injecting each DNA into both femurs. A control group was vaccinated with 1 mg of a mock plasmid (derived from pVax-1), into which no SFTS virus gene was inserted, by intradermally injecting the plasmid into both femurs (500 μg for each femur). Both the vaccinated groups and the control group were subjected to electroporation using an electroporator at 0.2 A immediately after intradermal injection. The vaccination was performed a total of three times, once every two weeks (vaccinated on days 0, 14 and 28).
Evaluation of T-Cell Immune Response Induced by Vaccine In SFTSV-Infected Medium-Sized Animal Models
Before vaccination and 2 weeks after 3rd vaccination, 5 ml of blood was sampled. PBMCs and serum were isolated therefrom, and SFTSV-specific T cell immune response (ELISpot assay) and antibody immune response (ELISA and neutralizing antibody assay) were measured. SFTSV-specific T cell immune response induced by the vaccine was evaluated by ELISpot assay. It was confirmed that a very strong SFTSV-specific T cell immune response against each SFTSV antigen depending on the kind of vaccine was induced by the SFTSV vaccine candidate in the medium-sized animal models. As shown in
Quantitative Evaluation of Antibody Immune Response and Neutralizing Antibody Titer Induced by Vaccine in SFTSV-Infected Medium-Sized Animal Models
The neutralizing antibody titer of antibody induced by the DNA vaccine was measured by PRNT50 assay. As shown in
Evaluation of Preventive Effect of SFTSV Preventive DNA Vaccine in SFTSV-Infected Medium-Sized Animal Models
To evaluate the preventive effect of each vaccine, the animals vaccinated with SFTSV vaccines against Gn/Gc, NP, NS and RdRp, respectively, were infected with a lethal dose of SFTSV, and then evaluated for clinical symptoms such as survival rate, SFTSV viral load, platelet count, body temperature and body weight changes, and ALT and AST changes.
As shown in
In addition, SFTSV viral load was measured by real-time PCR. The results are shown in
In addition, as shown in
As shown in
Meanwhile, it was confirmed that, after SFTSV infection, the animals of the control group showed a significant loss in body weight (relative body weight of 80% or less), whereas the animals of the vaccine groups showed no loss in body weight (relative body weight of 90% or less) (see
In addition, as shown in
As shown in
In addition, as shown in
As a result of evaluating the preventive effect of each of the Gn/Gc, NP, NS and RdRp antigens in the infected medium-sized animal (ferret) models as described above, it could be confirmed that the Gn/Gc DNA vaccine exhibited a significantly higher protective effect than other antigen-expressing DNA vaccines.
Although the present disclosure has been described in detail with reference to the specific features, it will be apparent to those skilled in the art that this detailed description is only of a preferred embodiment thereof, and does not limit the scope of the present disclosure. Thus, the substantial scope of the present disclosure will be defined by the appended claims and equivalents thereto.
The present disclosure relates to a vaccine composition for preventing or treating an infectious disease caused by severe fever with thrombocytopenia syndrome (SFTS) virus.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2018-0074418 | Jun 2018 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2019/007920 | 1/2/2020 | WO | 00 |
