Claims
- 1. A method of producing an antiviral vaccine for a predetermined vertebrate species selected from the group consisting of canine, feline, equine, porcine, avian, piscine, and human species, said vaccine comprising an amount of viral antigen effective in evoking systemic production of antibodies to said antigen, said method comprising the steps of:
propagating an antigen producing cell in a culture medium, said culture medium comprising serum wherein the serum consists essentially of serum from said predetermined vertebrate species; recovering an antigen concentrate from said culture medium; and, combining said antigen concentrate with a pharmaceutically acceptable carrier.
- 2. A vaccine prepared in accordance with the method of claim 1.
- 3. A feline vaccine prepared in accordance with the method of claim 1.
- 4. A human vaccine prepared in accordance with the method of claim 1.
- 5. A canine vaccine prepared in accordance with the method of claim 1.
- 6. An improved antiviral vaccine for a predetermined vertebrate species, said vaccine comprising
a homologous serum component; an antigenic component, the antigenic component produced in a culture comprising the homologous serum component; and a pharmaceutically acceptable carrier, wherein said composition is substantially free of bovine serum components and heterologous serum components.
- 7. The vaccine of claim 6 wherein the antigenic component comprises viral proteins.
- 8. The vaccine of claim 6 further comprising an adjuvant.
- 9. In a method for producing an antiviral vaccine for a predetermined vertebrate species consisting of canine, feline, equine, porcine, avian, piscine, and human species wherein an antigen concentrate is isolated from a culture medium used to propagate an antigen producing organism or cell, said culture medium comprising, serum and wherein the antigen concentrate is used to formulate said vaccine, the improvement which comprises using a culture medium wherein the serum component consists essentially of serum from the predetermined species.
- 10. A vaccine prepared in accordance with the improved method of claim 9.
- 11. A method for vaccinating a predetermined vertebrate species with an antiviral vaccine said method comprising the step of administering a vaccine prepared in accordance with the method of claim 1 in an amount effective to elicit an antivirally effective antibody titer in said predetermined vertebrate species.
- 12. The method of claim 11 wherein the pre-determined species is human.
- 13. The method of claim 11 wherein the pre-determined species is canine.
- 14. The method of claim 11 wherein the pre-determined species is feline.
- 15. A method for vaccinating a predetermined vertebrate species with an antiviral vaccine said method comprising the step of administering a vaccine prepared in accordance with the improved method of claim 9 in an amount effective to elicit an antivirally effective antibody titer in said predetermined vertebrate species.
- 16. A method of minimizing autoimmune side effects associated with vaccination of a warm-blooded vertebrate selected from canine, feline, equine, porcine, avian, piscine, and human species for protection against viral infection, said method comprising the steps of inoculating said vertebrate with an effective amount of an antiviral vaccine substantially free of heterologous species serum components.
- 17. The method of claim 16 wherein the antiviral vaccine is produced by
propagating an antigen producing cell in a culture medium, said culture medium comprising serum wherein the serum consists essentially of serum from said predetermined vertebrate species; recovering an antigen concentrate from said culture medium; and, combining said antigen concentrate with a pharmaceutically acceptable carrier.
- 18. The method of claim 16 wherein the warm-blooded vertebrate is a canine species.
Parent Case Info
[0001] This application is a continuation of U.S. application Ser. No. 09/462,258, filed Jan. 5, 2000, which is a U.S. national application of international application serial No. PCT/US98/14919 filed Jul. 17, 1998, which claims priority to U.S. provisional application serial No. 60/053,020 filed Jul. 18, 1997, which is expressly incorporated by reference herein.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60053020 |
Jul 1997 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09462258 |
Jan 2000 |
US |
Child |
09933308 |
Aug 2001 |
US |