Claims
- 1. An adjuvanted influenza vaccine for producing an antigenic response to influenza, in vivo, in mammals, said vaccine comprising:an effective amount of an influenza antigen and an adjuvant, said adjuvant comprising oil-containing paucilamellar lipid vesicles having nonphospholipid materials as the primary wall forming constituent, wherein said paucilamellar lipid vesicles have 2-10 bilayers surrounding an amorphous central cavity, and wherein said nonphospholipid materials are selected from the group consisting of polyoxyethylene fatty acid esters, polyoxyethylene fatty acid ethers, polyoxyethylene sorbitan esters, polyoxyethylene glyceryl mono- and diesters, glyceryl mono- and distearate, sucrose distearate, propylene glycol stearate, long chain acyl hexosamides, long chain acyl amino acid amides, long chain acyl amides, glyceryl mono-and diesters, dimethyl acyl amines, C12-C20 fatty alcohols, C12-C20 glycol monoesters, and C12-C20 fatty acids, and wherein said vaccine increases the antigenic response when compared to the antigen alone or the antigen adjuvanted with alum, wherein the antigen is mixed in solution with the adjuvant.
- 2. The vaccine of claim 1 wherein said antigen is encapsulated in said adjuvant.
- 3. The vaccine of claim 1 wherein said antigen is encapsulated in said amorphous central cavity.
- 4. The vaccine of claim 1 wherein said antigen is selected from the group consisting of antigens derived from formalin-inactivated whole virus, antigens derived from formalin-inactivated viral subunits, and antigens produced by recombinant DNA techniques.
- 5. The vaccine of claim 4 wherein said antigen is influenza A H3N2.
- 6. The vaccine of claim 1 wherein said paucilamellar lipid vesicles further comprise at least one sterol selected from the group consisting of cholesterol, cholesterol derivatives, hydrocortisone, and phytosterol.
- 7. The vaccine of claim 1 wherein said paucilamellar lipid vesicles comprise an amorphous central cavity containing a water immiscible oily material.
- 8. The vaccine of claim 7 wherein said water immiscible oily material is selected from the group consisting of soybean oil, squalene oil, squalane oil, sesame oil, olive oil, canola oil, corn oil, rapeseed oil, safflower oil, sunflower oil, fish oils, petrolatum, avocado oil, triglyceride oils and fats, flavor oils, and water insoluble vitamins.
REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of U.S. patent application Ser. No. 08/005,008 filed Jan. 15, 1993, entitled Method of Inhibiting Viral Reproduction, now abandoned and of Ser. No. 08/201,346, filed Feb. 24, 1994, entitled Vaccines Containing Paucilamellar Lipid Vesicles as Immunological Adjuvants, now abandoned the disclosures of which are incorporated herein by reference.
US Referenced Citations (15)
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
08/201346 |
Feb 1994 |
US |
Child |
08/840034 |
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US |
Parent |
08/005008 |
Jan 1993 |
US |
Child |
08/201346 |
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US |