Claims
- 1. A transgenic monocot plant selected from the group consisting of barley and wheat wherein at least a part of said plant comprises a recombinant nucleic acid comprising a promoter active in said part operably linked to a nucleic acid encoding a thioredoxin h polypeptide wherein said promoter is a seed or grain maturation-specific promoter and said thioredoxin h polypeptide is selected from the group consisting of barley, rice, Arabidopsis, soybean, wheat, tobacco and Brassica thioredoxin.
- 2. The transgenic plant of claim 1 wherein said part is a seed.
- 3. The transgenic plant of claim 1 wherein said part is a grain.
- 4. The transgenic plant of claim 1 wherein said promoter selected from the group consisting of rice glutelins, rice oryzins, rice prolamines, barley hordiens, wheat gliadins, wheat glutenins, maize zeins, maize glutelins, oat glutelins, sorghum kafirins, millet pennisetins, rye secalins, and maize embryo-specific globulin promoters.
- 5. The transgenic plant of claim 4 wherein said barley hordein promoter is selected from the group consisting of B-1hordein and D-hordein promoters.
- 6. The transgenic plant of claim 1 wherein said monocot plant is barley.
- 7. The transgenic plant of claim 1 wherein said thioredoxin h is from the group consisting of barley, wheat and rice thioredoxin h.
- 8. The transgenic plant of claim 1 wherein said recombinant nucleic acid further comprises a nucleic acid encoding a signal peptide operably linked to said promoter and said nucleic acid molecule encoding a thioredoxin h protein.
- 9. The transgenic plant of claim 8 wherein said signal peptide targets expression of the thioredoxin h polypeptide to an intracellular body.
- 10. The transgenic plant of claim 9 wherein said signal peptide is selected from the group consisting of B-1 hordein signal peptide.
- 11. The transgenic monocot seed or grain selected from the group consisting of barley and wheat comprising a recombinant nucleic acid comprising a promoter active in said seed or grain operably linked to a nucleic acid molecule encoding a barley, rice, Arabidopsis, soybean, wheat, tobacco, or Brassica thioredoxin h polypeptide wherein said promoter is a seed or grain maturation-specific promoter.
- 12. The transgenic seed or grain of claim 11 wherein said promoter is selected from the group consisting of rice glutelins, rice oryzins, rice prolamines, barley hordeins, wheat gliadins, wheat glutenins, maize zeins, maize glutelins, oat glutelins, sorghum kafirins, millet permisetins, rye sacalins, and maize embryo-specific globulin promoters.
- 13. The transgenic seed or grain of claim 11 wherein said barley hordein promoter is selected from the group consisting of B-1 hordein and D-hordein promoters.
- 14. The transgenic seed or grain of claim 11 wherein said seed or grain is barley.
- 15. The transgenic seed or grain of claim 11 wherein said thioredoxin h is selected from the group consisting of barley, wheat, and rice thioredoxin h.
- 16. The transgenic seed or grain of claim 11 wherein said recombinant nucleic acid further comprises a nucleic acid encoding signal peptide operably linked to said promoter and said nucleic molecule encoding a thioredoxin h protein.
- 17. The transgenic seed or grain of claim 16 wherein said signal peptide targets expression of the thioredoxin h polypeptide to an intracellular body.
- 18. The transgenic seed or grain of claim 17 wherein said signal peptide is selected for the group consisting of B-1 hordein and D-hordein signal peptide.
- 19. The transgenic plant of claim 6 wherein said plant is wheat.
- 20. The transgenic plant of claim 19 wherein said thioredoxin is wheat thioredoxin h.
- 21. The transgenic seed or grain of claim 14 wherein said seed or grain is barley.
- 22. The transgenic seed or grain of claim 21 wherein said thioredoxin is barley thioredoxin h.
- 23. The transgenic seed or grain of claim 14 wherein said seed or grain is wheat.
- 24. The transgenic seed or grain of claim 23 wherein said thioredoxin is wheat thioredoxin h.
- 25. The transgenic plant of claim 1 wherein said thioredoxin is Arabidopsis thioredoxin h.
- 26. The transgenic plant of claim 1 wherein said thioredoxin is soybean thioredoxin h.
- 27. The transgenic monocot seed or grain of claim 11 wherein said thioredoxin is Arabidopsis thioredoxin h.
- 28. The transgenic monocot seed or grain of claim 11 wherein said thioredoxin is soybean thioredoxin h.
- 29. The transgenic plant of claim 1 wherein said thioredoxin is tobacco thioredoxin h.
- 30. The transgenic plant of claim 1 wherein said thioredoxin is Brassica thioredoxin h.
- 31. The transgenic monocot seed or grain of claim 11 wherein said thioredoxin is tobacco thioredoxin h.
- 32. The transgenic monocot seed or grain of claim 11 wherein said thioredoxin is Brassica thioredoxin h.
Parent Case Info
This application claims the benefit of the filing date of application Ser. No. 60/126,736, filed Mar. 29, 1999, application Ser. No. 60/127,198, filed Mar. 31, 1999, application Ser. No. 60/169,162, filed Dec. 6, 1999, application Ser. No. 60/177,740 filed Jan. 21, 2000, and application Ser. No. 60/177,739, filed Jan. 21, 2000, all of which are expressly incorporated by reference in their entirety.
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT
This invention was made with Government support under Grant 9803835 from the U.S. Department of Agriculture. The Government has certain rights to this invention.
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Provisional Applications (5)
|
Number |
Date |
Country |
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60/177739 |
Jan 2000 |
US |
|
60/177740 |
Jan 2000 |
US |
|
60/169162 |
Dec 1999 |
US |
|
60/127198 |
Mar 1999 |
US |
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60/126736 |
Mar 1999 |
US |