Not applicable
The present invention relates to the field of methods and devices for the embolization of vascular aneurysms and similar vascular abnormalities. More specifically, the present invention relates to (a) an expansible vascular implant that is inserted into a vascular site such as an aneurysm to create an embolism therein; (b) a method of making the expansible implant; and (c) a method and an apparatus for embolizing a vascular site using the implant.
The embolization of blood vessels is desired in a number of clinical situations. For example, vascular embolization has been used to control vascular bleeding, to occlude the blood supply to tumors, and to occlude vascular aneurysms, particularly intracranial aneurysms. In recent years, vascular embolization for the treatment of aneurysms has received much attention. Several different treatment modalities have been employed in the prior art. U.S. Pat. No. 4,819,637—Dormandy, Jr. et al., for example, describes a vascular embolization system that employs a detachable balloon delivered to the aneurysm site by an intravascular catheter. The balloon is carried into the aneurysm at the tip of the catheter, and it is inflated inside the aneurysm with a solidifying fluid (typically a polymerizable resin or gel) to occlude the aneurysm. The balloon is then detached from the catheter by gentle traction on the catheter. While the balloon-type embolization device can provide an effective occlusion of many types of aneurysms, it is difficult to retrieve or move after the solidifying fluid sets, and it is difficult to visualize unless it is filled with a contrast material. Furthermore, there are risks of balloon rupture during inflation and of premature detachment of the balloon from the catheter.
Another approach is the direct injection of a liquid polymer embolic agent into the vascular site to be occluded. One type of liquid polymer used in the direct injection technique is a rapidly polymerizing liquid, such as a cyanoacrylate resin, particularly isobutyl cyanoacrylate, that is delivered to the target site as a liquid, and then is polymerized in situ. Alternatively, a liquid polymer that is precipitated at the target site from a carrier solution has been used. An example of this type of embolic agent is a cellulose acetate polymer mixed with bismuth trioxide and dissolved in dimethyl sulfoxide (DMSO). Another type is ethylene glycol copolymer dissolved in DMSO. On contact with blood, the DMSO diffuses out, and the polymer precipitates out and rapidly hardens into an embolic mass that conforms to the shape of the aneurysm. Other examples of materials used in this “direct injection” method are disclosed in the following U.S. Pat. No. 4,551,132—Pásztor et al.; U.S. Pat. No. 4,795,741—Leshchiner et al.; U.S. Pat. No. 5,525,334—Ito et al.; and U.S. Pat. No. 5,580,568—Greff et al.
The direct injection of liquid polymer embolic agents has proven difficult in practice. For example, migration of the polymeric material from the aneurysm and into the adjacent blood vessel has presented a problem. In addition, visualization of the embolization material requires that a contrasting agent be mixed with it, and selecting embolization materials and contrasting agents that are mutually compatible may result in performance compromises that are less than optimal. Furthermore, precise control of the deployment of the polymeric embolization material is difficult, leading to the risk of improper placement and/or premature solidification of the material. Moreover, once the embolization material is deployed and solidified, it is difficult to move or retrieve.
Another approach that has shown promise is the use of thrombogenic microcoils. These microcoils may be made of a biocompatible metal alloy (typically platinum and tungsten) or a suitable polymer. If made of metal, the coil may be provided with Dacron fibers to increase thrombogenicity. The coil is deployed through a microcatheter to the vascular site. Examples of microcoils are disclosed in the following U.S. Pat. No. 4,994,069—Ritchart et al.; U.S. Pat. No. 5,133,731—Butler et al.; U.S. Pat. No. 5,226,911—Chee et al.; U.S. Pat. No. 5,312,415—Palermo; U.S. Pat. No. 5,382,259—Phelps et al.; U.S. Pat. No. 5,382,260—Dormandy, Jr. et al.; U.S. Pat. No. 5,476,472—Dormandy, Jr. et al.; U.S. Pat. No. 5,578,074—Mirigian; U.S. Pat. No. 5,582,619—Ken; U.S. Pat. No. 5,624,461—Mariant; U.S. Pat. No. 5,645,558—Horton; U.S. Pat. No. 5,658,308—Snyder; and U.S. Pat. No. 5,718,711—Berenstein et al.
The microcoil approach has met with some success in treating small aneurysms with narrow necks, but the coil must be tightly packed into the aneurysm to avoid shifting that can lead to recanalization. Microcoils have been less successful in the treatment of larger aneurysms, especially those with relatively wide necks. A disadvantage of microcoils is that they are not easily retrievable; if a coil migrates out of the aneurysm, a second procedure to retrieve it and move it back into place is necessary. Furthermore, complete packing of an aneurysm using microcoils can be difficult to achieve in practice.
A specific type of microcoil that has achieved a measure of success is the Guglielmi Detachable Coil (“GDC”). The GDC employs a platinum wire coil fixed to a stainless steel guidewire by a solder connection. After the coil is placed inside an aneurysm, an electrical current is applied to the guidewire, which heats sufficiently to melt the solder junction, thereby detaching the coil from the guidewire. The application of the current also creates a positive electrical charge on the coil, which attracts negatively-charged blood cells, platelets, and fibrinogen, thereby increasing the thrombogenicity of the coil. Several coils of different diameters and lengths can be packed into an aneurysm until the aneurysm is completely filled. The coils thus create and hold a thrombus within the aneurysm, inhibiting its displacement and its fragmentation.
The advantages of the GDC procedure are the ability to withdraw and relocate the coil if it migrates from its desired location, and the enhanced ability to promote the formation of a stable thrombus within the aneurysm. Nevertheless, as in conventional microcoil techniques, the successful use of the GDC procedure has been substantially limited to small aneurysms with narrow necks.
Still another approach to the embolization of an abnormal vascular site is the injection into the site of a biocompatible hydrogel, such as poly (2-hydroxyethyl methacrylate) (“pHEMA” or “PHEMA”); or a polyvinyl alcohol foam (“PAF”). See, e.g., Horak et al., “Hydrogels in Endovascular Embolization. II. Clinical Use of Spherical Particles”, Biomaterials, Vol. 7, pp. 467-470 (November, 1986); Rao et al., “Hydrolysed Microspheres from Cross-Linked Polymethyl Methacrylate”, J. Neuroradiol., Vol. 18, pp. 61-69 (1991); Latchaw et al., “Polyvinyl Foam Embolization of Vascular and Neoplastic Lesions of the Head, Neck, and Spine”, Radiology, Vol. 131, pp. 669-679 (June, 1979). These materials are delivered as microparticles in a carrier fluid that is injected into the vascular site, a process that has proven difficult to control.
A further development has been the formulation of the hydrogel materials into a preformed implant or plug that is installed in the vascular site by means such as a microcatheter. See, e.g., U.S. Pat. No. 5,258,042—Mehta and U.S. Pat. No. 5,456,693—Conston et al. These types of plugs or implants are primarily designed for obstructing blood flow through a tubular vessel or the neck of an aneurysm, and they are not easily adapted for precise implantation within a sack-shaped vascular structure, such as an aneurysm, so as to fill substantially the entire volume of the structure.
There has thus been a long-felt, but as yet unsatisfied need for an aneurysm treatment device and method that can substantially fill aneurysms of a large range of sizes, configurations, and neck widths with a thrombogenic medium with a minimal risk of inadvertent aneurysm rupture or blood vessel wall damage. There has been a further need for such a method and device that also allow for the precise locational deployment of the medium, while also minimizing the potential for migration away from the target location. In addition, a method and device meeting these criteria should also be relatively easy to use in a clinical setting. Such ease of use, for example, should preferably include a provision for good visualization of the device during and after deployment in an aneurysm.
Broadly, a first aspect of the present invention is a device for occluding a vascular site, such as an aneurysm, comprising a conformal vascular implant, formed of an expansible material, that is compressible from an initial configuration for insertion into the vascular site by means such as a microcatheter while the implant is in a compressed configuration, and that is expansible in situ into an expanded configuration in which it substantially fills the vascular site, thereby to embolize the site, wherein the initial configuration of the implant is a scaled-down model of the vascular site.
In a preferred embodiment, the implant is made of a hydrophilic, macroporous, polymeric, hydrogel foam material, in particular a water-swellable foam matrix formed as a macroporous solid comprising a foam stabilizing agent and a polymer or copolymer of a free radical polymerizable hydrophilic olefin monomer cross-linked with up to about 10% by weight of a multiolefin-functional cross-linking agent. The material is modified, or contains additives, to make the implant visible by conventional imaging techniques.
Another aspect of the present invention is a method of manufacturing a vascular implant, comprising the steps of: (a) imaging a vascular site by scanning the vascular site to create a digitized scan data set; (b) using the scan data set to create a three-dimensional digitized virtual model of the vascular site; and (c) forming a vascular implant device in the form of a physical model of the vascular site, using the virtual model, the implant being formed of a compressible and expansible biocompatible foam material. In a specific embodiment, the forming step (c) comprises the substeps of: (c)(1) using the virtual model to create a scaled-down, three-dimensional physical mold of the vascular site; and (c)(2) using the mold to create a vascular implant in the form of a scaled-down physical model of the vascular site.
In the preferred embodiment of the method of manufacturing the implant, the imaging step is performed with a scanning technique such as computer tomography (commonly called “CT” or “CAT”), magnetic resonance imaging (MRI), magnetic resonance angiography (MRA), or ultrasound. Commercially-available software, typically packaged with and employed by the scanning apparatus, reconstructs the scan data set created by the imaging into the three-dimensional digitized model of the vascular site. The digitized model is then translated, by commercially-available software, into a form that is useable in a commercially available CAD/CAM program to create the scaled-down physical mold by means of stereolithography. A suitable implant material, preferably a macroporous hydrogel foam material, is injected in a liquid or semiliquid state into the mold. Once solidified, the hydrogel foam material is removed from the mold as an implant in the form of a scaled-down physical model of the vascular site.
A third aspect of the present invention is a method for embolizing a vascular site, comprising the steps of (a) passing a microcatheter intravascularly so that its distal end is in a vascular site; (b) providing a vascular implant in the form of a scaled-down physical model of the vascular site, the implant being formed of a compressible and expansible biocompatible foam material; (c) compressing the implant into a compressed configuration dimensioned to pass through a microcatheter; (d) passing the implant, while it is in its compressed configuration, through the microcatheter so that the implant emerges from the distal end of the microcatheter into the vascular site; and (e) expanding the implant in situ substantially to fill the vascular site.
The apparatus employed in the embolization method comprises an elongate, flexible deployment element dimensioned to fit axially within an intravascular microcatheter; a filamentous implant retention element disposed axially through the deployment element from its proximal end to its distal end; and an implant device removably attached to the distal end of the retention element.
The implant device, in its preferred embodiment, is formed of a moldable, hydrophilically expansible, biocompatible foam material that has an initial configuration in the form of a scaled-down physical model of the vascular site, that is compressible into a compressed configuration that fits within the microcatheter, and that is hydrophilically expansible into an expanded configuration in which it is dimensioned substantially to conform to and fill the vascular site. Alternatively, the implant device may be formed of a non-hydrophilic foam material having an initial configuration that is substantially the same size and shape as the vascular site, and that restores itself to its initial configuration after it is released from its compressed configuration.
The retention element is preferably a flexible wire having a distal end configured to releasably engage the implant device while the implant device is in its compressed configuration, thus to retain the implant device within the distal end of the microcatheter while the distal end of the microcatheter is inserted into the vascular site. The wire is movable axially with the deployment element in the distal direction to expose the implant from the distal end of the microcatheter, and is movable proximally with respect to the deployment element to urge the implant device against the distal end of the deployment element, thereby push the implant device off of the wire. Thus released into the vascular site, the implant device expands into an expanded configuration in which it substantially conforms to and fills the vascular site.
The present invention provides a number of significant advantages. Specifically, the present invention provides an effective vascular embolization implant that can be deployed within a vascular site with excellent locational control, and with a lower risk of vascular rupture, tissue damage, or migration than with prior art implant devices. Furthermore, the implant device, by being modelled on the actual vascular site in which it is to be implanted, effects a conformal fit within the site that promotes effective embolization, and yet its ability to be delivered to the site in a highly compressed configuration facilitates precise and highly controllable deployment with a microcatheter. In addition, the method of fabricating the implant device, by modeling it on each individual site, allows implant devices to be made that can effectively embolize vascular sites having a wide variety of sizes, configurations, and (in the particular case of aneurysms) neck widths. These and other advantages will be readily appreciated from the detailed description that follows.
The Method of Manufacturing a Vascular Implant.
A first aspect of the present invention is a method of manufacturing a vascular implant device. The steps of a preferred embodiment of the manufacturing method are shown as a sequence of descriptive boxes in the flow chart of
The first step, shown in box 10 of
The result of the imaging step is a digitized scan data set that is stored in a computer memory, from which the data set is retrieved for operation of the next step: computerized reconstruction of a three-dimensional digitized virtual model of the vascular site (box 12 of
The digitized, three-dimensional virtual model is then translated into a form in which it can be employed in a commercially-available CAD/CAM program (box 14) which controls a stereolithography process (box 16) to create a mold for forming an implant device. The translation of the virtual model is performed by software that is commercially available, for example, from Cyberform International, Inc., of Richardson, Tex., and from Stratasys, Inc., of Minneapolis, Minn. The mold (not shown) is preferably scaled-down from the dimensions of the vascular site, with a scale of about 1:2 to about 1:6, with about 1:4 being preferred. Alternatively, the mold may be made “life size” (i.e., 1:1); that is, a full-size or nearly full-size replica of the vascular site. The mold is used in the fabrication of a vascular implant device by conventional molding techniques (box 18).
The Implant Device.
A vascular implant device 20, in accordance with the present invention, is shown in
In the preferred embodiment, the implant device 20 is made of a biocompatible, macroporous, hydrophilic hydrogel foam material, in particular a water-swellable foam matrix formed as a macroporous solid comprising a foam stabilizing agent and a polymer or copolymer of a free radical polymerizable hydrophilic olefin monomer cross-linked with up to about 10% by weight of a multiolefin-functional cross-linking agent. A suitable material of this type is described in U.S. Pat. No. 5,570,585—Park et al., the disclosure of which is incorporated herein by reference. Another suitable material is a porous hydrated polyvinyl alcohol foam (PAF) gel prepared from a polyvinyl alcohol solution in a mixed solvent consisting of water and a water-miscible organic solvent, as described, for example, in U.S. Pat. No. 4,663,358—Hyon et al., the disclosure of which is incorporated herein by reference. Still another suitable material is PHEMA, as discussed in the references cited above. See, e.g., Horak et al., supra, and Rao et al., supra. The foam material preferably has a void ratio of at least about 90%, and its hydrophilic properties are such that it has a water content of at least about 90% when fully hydrated.
In a preferred embodiment, the implant device 20, in its initial, precompressed configuration, will have the same configuration as the vascular site, but it will be smaller, by a factor of approximately two to approximately six. The material of the implant device 20, and its initial size, are selected so that the implant device 20 is swellable or expansible to approximately the size of the vascular site, primarily by the hydrophilic absorption of water molecules from blood plasma, and secondarily by the filling of its pores with blood. The result is an expanded configuration for the implant device 20, as shown in
Alternatively, the implant 20 device can be molded so that in its initial, precompressed configuration, it is “life size”, i.e., approximately the same size as the vascular site. In this case, the preferred material is a compressible, non-hydrophilic polymeric foam material, such as polyurethane. In actual clinical practice, a non-hydrophilic implant device 20 would advantageously be made slightly smaller than actual life size, to accommodate swelling due to the filling of the pores.
The foam material of the implant device 20, whether hydrophilic or non-hydrophilic, is advantageously modified, or contains additives, to make the implant 20 visible by conventional imaging techniques. For example, the foam can be impregnated with a water-insoluble radiopaque material such as barium sulfate, as described by Thanoo et al., “Radiopaque Hydrogel Microspheres”, J Microencapsulation, Vol. 6, No. 2, pp. 233-244 (1989). Alternatively, the hydrogel monomers can be copolymerized with radiopaque materials, as described in Horák et al., “New Radiopaque PolyHEMA-Based Hydrogel Particles”, J. Biomedical Materials Research, Vol. 34, pp. 183-188 (1997).
Whatever the material from which the implant device 20 is made, the implant device 20 must be compressible to a fraction of its initial size, preferably into a substantially cylindrical or lozenge-shaped configuration, as shown in
The Method and Apparatus for Embolizing a Vascular Site.
The method of embolizing a vascular site using the implant device 20 is performed using an implanting apparatus 30, a preferred embodiment of which is shown in
The implant device 20, in its compressed configuration, has a maximum outside diameter that is less than the inside diameter of the microcatheter 32, so that the implant device 20 can be passed through the microcatheter 32. The implant device 20 is preferably compressed and “set”, as described above, before it is inserted into the microcatheter 32.
First, as shown in
The implant deployment element 34, with the implant device 20 extending from its distal end, is then passed through the microcatheter 32, as described above, until the implant device 20 emerges from the distal end of the microcatheter 32 into the vascular site 40, as shown in
Finally, when the expansion of the implant device 20 is well underway (and not necessarily when it is completed), the retention wire 22 is pulled proximally with respect to the implant deployment element 34, causing the implant device to be pushed off the end of the installation wire 22 by means of the pressure applied to it by the distal end of the implant deployment element 34. The implant device 20, now free of the implanting apparatus 30, as shown in
While a preferred embodiment of the invention has been described above, a number of variations and modifications may suggest themselves to those skilled in the pertinent arts. For example, instead of custom-fabricating the implant device for each patient, implant devices in a variety of “standard” sizes and shapes may be made, and a particular implant device then selected for a patient based on the imaging of the vascular site. In this case, the fabrication method shown in
This application is a Continuation of application Ser. No. 12/337,520 filed Dec. 17, 2008, issuing as U.S. Pat. No. 7,799,047 on Sep. 21, 2010, which is a Continuation of U.S. patent application Ser. No. 11/733,697 filed Apr. 10, 2007, now U.S. Pat. No. 7,483,558 issued Jan. 27, 2009, which is a Continuation of application Ser. No. 10/320,033, filed Dec. 16, 2002, now U.S. Pat. No. 7,201,762 issued Apr. 10, 2007, which is a Continuation of application Ser. No. 09/730,071, filed Dec. 5, 2000, now U.S. Pat. No. 6,500,190 issued Dec. 31, 2002, which is a Continuation of application Ser. No. 09/110,816, filed Jul. 6, 1998, now U.S. Pat. No. 6,165,193 issued Dec. 12, 2000, all of which are hereby incorporated herein by reference.
Number | Name | Date | Kind |
---|---|---|---|
3709842 | Stoy | Jan 1973 | A |
4365621 | Brundin | Dec 1982 | A |
4436684 | White | Mar 1984 | A |
4506393 | Murphy | Mar 1985 | A |
4509504 | Brundin | Apr 1985 | A |
4529739 | Scott et al. | Jul 1985 | A |
4551132 | Pásztor et al. | Nov 1985 | A |
4663358 | Hyon et al. | May 1987 | A |
4734097 | Tanabe et al. | Mar 1988 | A |
4795741 | Leshchiner et al. | Jan 1989 | A |
4819637 | Dormandy, Jr. et al. | Apr 1989 | A |
4873707 | Robertson | Oct 1989 | A |
4940412 | Blumenthal | Jul 1990 | A |
4994069 | Ritchart et al. | Feb 1991 | A |
5007936 | Woolston | Apr 1991 | A |
5133731 | Butler et al. | Jul 1992 | A |
5156777 | Kaye | Oct 1992 | A |
5184306 | Erdman et al. | Feb 1993 | A |
5226911 | Chee et al. | Jul 1993 | A |
5258042 | Mehta | Nov 1993 | A |
5274565 | Reuben | Dec 1993 | A |
5312415 | Palermo | May 1994 | A |
5320639 | Rudnick | Jun 1994 | A |
5350397 | Palermo et al. | Sep 1994 | A |
5354290 | Gross | Oct 1994 | A |
5360446 | Kennedy | Nov 1994 | A |
5365996 | Crook | Nov 1994 | A |
5382259 | Phelps et al. | Jan 1995 | A |
5382260 | Dormandy, Jr. et al. | Jan 1995 | A |
5448489 | Reuben | Sep 1995 | A |
5452407 | Crook | Sep 1995 | A |
5456693 | Conston et al. | Oct 1995 | A |
5476472 | Dormandy, Jr. et al. | Dec 1995 | A |
5525334 | Ito et al. | Jun 1996 | A |
5541234 | Unger et al. | Jul 1996 | A |
5573994 | Kabra et al. | Nov 1996 | A |
5578074 | Mirigian | Nov 1996 | A |
5580568 | Greff et al. | Dec 1996 | A |
5582619 | Ken | Dec 1996 | A |
5624461 | Mariant | Apr 1997 | A |
5624685 | Takahashi et al. | Apr 1997 | A |
5645558 | Horton | Jul 1997 | A |
5658308 | Snyder | Aug 1997 | A |
5698213 | Jamiolkowski et al. | Dec 1997 | A |
5718711 | Berenstein et al. | Feb 1998 | A |
5738667 | Solar | Apr 1998 | A |
5750585 | Park et al. | May 1998 | A |
5752974 | Rhee et al. | May 1998 | A |
5762125 | Mastrorio | Jun 1998 | A |
5762315 | Eggleston | Jun 1998 | A |
5823198 | Jones et al. | Oct 1998 | A |
5825908 | Pieper et al. | Oct 1998 | A |
5826587 | Berenstein et al. | Oct 1998 | A |
5911731 | Pham et al. | Jun 1999 | A |
5935148 | Villar et al. | Aug 1999 | A |
5957948 | Mariant | Sep 1999 | A |
6015424 | Rosenbluth et al. | Jan 2000 | A |
6066325 | Wallace et al. | May 2000 | A |
6096021 | Helm et al. | Aug 2000 | A |
6113629 | Ken | Sep 2000 | A |
6124273 | Drohan et al. | Sep 2000 | A |
6165193 | Greene et al. | Dec 2000 | A |
6210432 | Solem et al. | Apr 2001 | B1 |
6299619 | Greene, Jr. et al. | Oct 2001 | B1 |
6312421 | Boock | Nov 2001 | B1 |
6350463 | Herman et al. | Feb 2002 | B1 |
6463317 | Kucharczyk et al. | Oct 2002 | B1 |
6500190 | Greene et al. | Dec 2002 | B2 |
6605111 | Bose et al. | Aug 2003 | B2 |
6605294 | Sawhney | Aug 2003 | B2 |
7029487 | Greene, Jr. et al. | Apr 2006 | B2 |
7109255 | Loomis et al. | Sep 2006 | B2 |
7201762 | Greene et al. | Apr 2007 | B2 |
7483558 | Greene et al. | Jan 2009 | B2 |
7799047 | Greene et al. | Sep 2010 | B2 |
Number | Date | Country |
---|---|---|
WO 8911257 | Nov 1989 | WO |
WO 9726939 | Jul 1997 | WO |
WO 9816266 | Apr 1998 | WO |
WO 9923954 | May 1999 | WO |
WO 9956783 | Nov 1999 | WO |
Entry |
---|
Chirila, Traian et al., “Poly(2-hydroxyethyl methacrylate) sponges as implant materials: in vivo and in vitro evaluation of cellular invasion,” Biomaterials 1993, pp. 26-38, vol. 14, No. 1. |
Horák, Daniel et al., “Hydrogels in endovascular embolization. II. Clinical use of spherical particles,” Biomaterials 1986, pp. 467-420, vol. 7, No. 6. |
Horák, D. et al., “New radiopaque poly-HEMA-based hydrogel particles,” Journal of Biomedical Materials Research, 1997, pp. 183-188, vol. 34. |
Latchaw, Richard e. et al., “Polyvinyl Foam Embolization of Vascular and Neoplastic Lesions of the Head, Neck, and Spine,” Radiology, Jun. 1979, pp. 669-678, vol. 131. |
Chithambara Thanoo, B. et al., “Radiopaque hydrogel microspheres,” J. Microencapsulation, 1989, pp. 233-244, vol. 6, No. 2. |
McGurk M. et al., “Rapid prototyping techniques for anatomical modeling in medicine,” Ann R. Coll Surg Engl, 1997, vol. 79, pp. 169-174. |
Rao, V.R.K. et al., Hydrolysed Microspheres From Cross-Linked Polymethyl Methacrylate (Hydrogel), J. Neuroradio, 1991, vol. 18, pp. 61-69. |
Robertson, Douglas D. et al., “Design of Custom Hip Stem Prostheses Using Three-Dimensional CT Modeling,” Journal of Computer Assisted Tomography, Sep./Oct. 1987, vol. 11, No. 5, pp. 804-809. |
McPherson, David D., “Three-Dimensional Arterial Imaging,” Scientific American Science and Medicine, Mar./Apr. 1996, pp. 22-33. |
MacDonald, Warren et al., “Designing an Implant by CT Scanning and Solid Modeling,” Journal of Bone and Joint Surgery, Mar. 1986, vol. 68-B, No. 2, pp. 208-212. |
Wake Conley M. et al., “Dynamics of Fibrovascular Tissue Ingrowth in Hydrogel Forams,” Cell Transplantation, 1995, vol. 4, No. 3, pp. 275-279. |
Woerly, S. et al., “Intracerebral implantation of synthetic polymer/biopolymer matrix: a new perspective for brain repair,” Biomaterials, Mar. 1990, vol. 11, No. 2, pp. 97-107. |
Lewis, Sr., Richard J., Hawley's Condensed Chemical Dictionary, Twelfth Edition, 1993, pp. 351-351, Van Nostrand Reinhold, New York. |
Number | Date | Country | |
---|---|---|---|
20110005062 A1 | Jan 2011 | US |
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