Claims
- 1. An isolated nucleic acid molecule having a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO.: 1 or SEQ ID NO.: 2; b) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO.: 1 or SEQ ID NO.: 2; c) a nucleotide sequence comprising a sequence having at least 70% identity to the sequence set forth in SEQ ID NO.: 1 or SEQ ID NO.: 2, or a fragment thereof, wherein said sequence regulates transcription; and, d) a nucleotide sequence that hybridizes under stringent conditions to sequence of a), b), c), or a complement thereof.
- 2. An isolated promoter that is capable of driving transcription in a vascular tissue-preferred manner, wherein the promoter is natively associated with DNA coding for Prunus serotina prunasin hydrolase.
- 3. An expression cassette comprising the nucleotide sequence of claim 1 operably linked to a heterologous nucleotide sequence of interest.
- 4. An expression vector comprising the expression cassette of claim 3.
- 5. A host cell having stably incorporated into its genome the expression cassette of claim 3.
- 6. A plant cell having stably incorporated into its genome the expression cassette of claim 3.
- 7. The plant cell of claim 5, wherein said plant cell is from a dicotyledonous plant.
- 8. The plant cell of claim 5, wherein said plant cell is from a monocotyledonous plant.
- 9. A plant having stably incorporated into its genome a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO.:1 or SEQ ID NO.:2; b) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO.:1 or SEQ ID NO.: 2; c) a nucleotide sequence comprising a sequence having at least 70% identity to the sequence set forth in SEQ ID NO.:1 or SEQ ID NO.: 2, or a fragment thereof, wherein said sequence regulates transcription; and, d) a nucleotide sequence that hybridizes under stringent conditions to sequence of a), b), c), or a complement thereof.
- 10. The plant of claim 9, wherein said plant is from a dicotyledonous plant.
- 11. The plant of claim 9, wherein said plant is from a monocotyledonous plant.
- 12. A seed of the plant of claim 9, wherein the seed comprises the nucleotide sequence of claim 1.
- 13. A method for expressing a heterologous nucleotide sequence in a plant, said method comprising stably integrating into a plant cell a heterologous nucleotide sequence of interest operably linked to a promoter, wherein the promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO.: 1 or SEQ ID NO.: 2; b) a nucleotide sequence comprising at least 100 contiguous nucleotides of the sequence set forth in SEQ ID NO.: 1 or SEQ ID NO.: 2; c) a nucleotide sequence comprising a sequence having at least 70% identity to the sequence set forth in SEQ ID NO.: 1 or SEQ ID NO.: 2, or a fragment thereof, wherein said sequence regulates transcription; and, d) a nucleotide sequence that hybridizes under stringent conditions to the complement of a sequence of a), b), c), or a complement thereof.
- 14. The method of claim 13, wherein said plant is dicotyledonous.
- 15. The method of claim 13, wherein said plant is a monocotyledonous.
- 16. The method of claim 13, wherein said heterologous nucleotide sequence is preferentially expressed in vascular tissue.
- 17. The method of claim 16, wherein said vascular tissue is phloem tissue.
- 18. The method of claim 13, wherein the heterologous nucleotide sequence of interest encodes a polypeptide having antipathogenic activity.
- 19. The method of claim 18, wherein the heterologous nucleotide sequence encodes a polypeptide having antipathogenic activity towards insects.
- 20. The method of claim 19, wherein said heterologous nucleotide sequence encodes a Bacillus thuringiensis (B.t.) toxic polypeptide or a chimeric B.t. toxic polypeptide.
- 21. The method of claim 19, wherein said heterologous nucleotide sequence of interest encodes a polypeptide selected from the group consisting of lectins and lipoxidases.
- 22. The method of claim 19, wherein said heterologous nucleotide sequence of interest encodes a polypeptide selected from the group consisting of an insect chitinase and an insecticidal polypeptide.
- 23. The method of claim 13, wherein said heterologous nucleotide sequence of interest encodes a polypeptide capable of regulating vascular tissue loading.
- 24. The method of claim 23, wherein said polypeptide is selected from the group consisting of sucrose synthases, sucrose transporters, galactinol synthases, K+ channels, amino acid transporters, H+ ATPases, amino acid permeases, sulfate transporters, fructosyltransferases, and phloem carbohydrate regulators.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60/305,362, filed Jul. 13, 2001.
Provisional Applications (1)
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Number |
Date |
Country |
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60305362 |
Jul 2001 |
US |