Claims
- 1. A nucleotide sequence which hybridizes preferentially to the nucleic acid sequence shown in FIG. 1 (SEQ ID NO 1), or a fragment of said nucleic acid sequence, under appropriate hybridization conditions, said nucleotide sequence being about 20-50 bases in length and wherein hybridization of said nucleotide sequence is an indication of strains of V. vulnificus.
- 2. The nucleotide sequence of claim 1 wherein said nucleotide sequence has a lack of internal repeats.
- 3. The nucleotide sequence of claim 1 wherein said nucleotide sequence is one of a pair of PCR primers.
- 4. A probe comprising the nucleotide sequence of claim 1 and a detectable moiety.
- 5. A method of detecting the presence of V. vulnificus in a sample comprising the steps of:(a) contacting the sample with the nucleotide sequence of claim 1; (b) imposing hybridization conditions on the sample and said nucleotide sequence to allow the formation of a hybridization product between said nucleotide sequence and DNA or RNA from V. vulnificus; and (c) detecting any hybridization product as an indication of the presence of V. vulnificus in the sample.
- 6. The nucleotide sequence of claim 1 wherein said nucleotide sequence hybridizes under stringent conditions to the wza gene of V. vulnificus.
- 7. A kit for detecting the presence of V. vulnificus in a sample comprising (a) a pair of PCR primers according to claim 3, (b) a suitable polymerase, and (c) buffers and reagents usable in PCR.
- 8. The probe according to claim 4 wherein the detectable moiety is selected from the group consisting of biotin, an enzyme, and a fluorescent molecule.
- 9. The probe according to claim 8 wherein the detectable moiety is a fluorescent molecule selected from the group consisting of fluorescein and rhodamine.
- 10. An isolated nucleotide sequence which hybridizes preferentially to the capsular polysaccaride transport gene from V. vulnificus, said nucleotide sequence being about 20-50 bases in length.
- 11. The nucleotide sequence of claim 10 wherein said nucleotide sequence is or is complementary to a nucleotide sequence consisting of about 20-50 consecutive nucleotides from a nucleotide sequence shown in FIG. 1 (SEQ ID NO 1).
- 12. The nucleic acid sequence shown in FIG. 1 (SEQ ID NO 1) in isolated form.
CROSS-REFERENCE TO RELATED APPLICATION
This is a continuation-in-part of PCT/US98/01467 filed Jun. 19, 1998 which is a continuation-in-part of U.S. Provisional Application Serial No. 60/050,243 filed on Jun. 19, 1997, the contents of which are incorporated herein by reference.
FEDERAL SPONSORSHIP OF INVENTION
The U.S. Government has a paid-up license in this invention awarded under the Merit Review Program by the United States Veterans Administration.
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|
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|
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Entry |
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Powell et al. Release of Tumor Necrosis Factor Alpha in Response to Vibrio vulnificus capsular Polysaccharides in In Vivo and In Vitro Models. Infection and Immunity, 1997, vol. 65, No. 9, pp. 3713-3718. |
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Provisional Applications (1)
|
Number |
Date |
Country |
|
60/050243 |
Jun 1997 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
PCT/US98/01467 |
Jun 1998 |
US |
Child |
09/205283 |
|
US |