Patent Application
20250000392
The present disclosure is directed to a wearable skin-mountable extended gate field effect transistor (EGFET) device for detecting and/or measuring a constituent of an interstitial fluid from the skin of a subject.
Smart wearable devices based on sweat or interstitial fluids (ISF) biosensing have gained considerable advantage in monitoring multi-physiological indexes and biomarkers, such as minerals, trace elements, lactic acid, urea, and volatile organic compounds, especially in view of the growing demand for convenient remote healthcare monitoring and telemedicine.
Currently available systems for analyzing body fluids biomarkers rely on collecting a sample followed by an elaborate offline analysis, which often requires the use of bulky laboratory devices. These systems do not allow continuous measurements and continuous monitoring of fluid composition, which are of supreme importance for the early detection of chronic diseases and play a crucial role in all treatment and prevention strategies.
One of the most common methods to measure biomarkers levels is by taking a blood sample, which is not only painful to collect, but is also time-consuming procedure. More importantly, this method requires trained personnel and does not provide continuous measurements. Compared to blood, ISF sampling is easier, painless, and enables safer online and in-situ biomarker analysis for long-term monitoring, as well as eliminating the need for extraction methods or dealing with blood clots. Compared to sweat biosensors that require tedious extraction methods and are not time-efficient, ISF could provide biomarker analysis without the need for excessive sweating exercises or being affected by confounding factors linked with the sweat conditions, such as poor sweat rates, sample evaporation, sample freshness, and/or contamination from the skin. As for urine, although easily accessible, it cannot be sampled in real time, making it difficult for online monitoring.
One of the highly effective, painless approaches to measure biomarkers levels inside the ISF is the use of microneedles (MNs). Such sharp microscopic structures are barely visible and can easily puncture the stratum corneum (the outermost layer of the skin) and reach the viable epidermis to enable real-time analyte measurements.
Nevertheless, most common MNs are typically constrained to rigid structures, limiting their use in stretchable or flexible devices. Indeed, the design and development of flexible microneedle devices has been challenged by low mechanical stability, breakability upon attachment to skin, making them unsuitable for long-term monitoring or for real-life clinical applications (L. Ren, et al., Fabrication of Flexible Microneedle Array Electrodes for Wearable Bio-Signal Recording. Sensors 18, 1191 (2018); K. Takeuchi et al., Flexible and porous microneedles of PDMS for continuous glucose monitoring. Biomedical Microdevices 22, 79 (2020)). Additionally, rigid MNs can easily detach from the skin and are not stable for long term measurements. Fabricating fully stretchable-soft microneedles patches typically results in mechanically weak structures that can break easily when touching the skin, and not able to reach the ISF and provide accurate measurement of the biomarkers for long periods of time.
Ciui et al., 2018 developed a wearable bendable bandage-based sensor and a minimally invasive microneedle biosensor for rapid screening of skin melanoma by detecting the presence of the tyrosinase (TYR) enzyme cancer biomarker in the presence of its catechol substrate, immobilized on the transducer surface. To overcome the mechanical challenge, the TYR bandage sensor was fabricated by screen printing stress enduring inks directly onto the soft fabric of a medical bandage. The utility of the developed microneedle platform was confirmed in vitro in porcine skin tissues, while a flexible ultralight electronic board was used for wireless data collection, processing, and transmission. However, although the fabrication method provided high resiliency against mechanical strains, screen printing suffers from low pattern resolution and inject printing is limited by the synthesis of conductive inks and nozzle-clogging problems (Ciui, B., et al. (2018). Wearable wireless tyrosinase bandage and microneedle sensors: toward melanoma screening. Advanced healthcare materials, 7 (7), 1701264).
Wang et al. (2016) fabricated a drug delivery skin patch using an array of bendable microneedles. To tolerate the deformation associated with skin stretching at the same time with maintaining the microneedles integrity when the skin patch is applied on a joint such as elbow or knuckle, the microneedle array was designed to have a soft base and a rigid sharp tip. In cases for which the skin patch is applied on the arm or abdomen, this design is said to enable the microneedle to be dragged out of the skin instead of leaving a broken needle in it when lateral movement between the microneedle patch and skin surface occurs (Wang, H., et al. (2016). Toward self-powered wearable adhesive skin patch with bendable microneedle array for transdermal drug delivery. Advanced Science, 3 (9), 1500441).
Numerous methods for making flexible microneedles were examined aiming to resolve the mechanical conflict. Typically, these methods were costly or involved multiple complex preparation steps (R. Wang et al. (2012) “A Flexible Microneedle Electrode Array With Solid Silicon Needles,” in Journal of Microelectromechanical Systems, vol. 21, no. 5, pp. 1084-1089; Srivastava, A. K., et al. (2015). Long term biopotential recording by body conformable photolithography fabricated low-cost polymeric microneedle arrays. Sensors and Actuators A: Physical, 236, 164-172).
In cases where the MNs has to be used as a transistor, contact of the ISF with the device increases the potential effect of the noises on the electrical measurement. However, potentiometric sensing based on field-effect transistors (FETs) has shown promise for advanced biosensing. In comparison with other techniques, such FET platforms have the advantage of fast response time, high sensitivity, and easy fabrication with well-established electronic manufacturing processes. Up to now, FET biosensors have been developed for many analytes, most of these designs are based on flat FETs and rigid devices, yet some attempts have been made to conform the FETs to more flexible devices.
Kajisa and Sakata (2017) developed highly sensitive and biocompatible glucose sensor using a FET with a functionalized hydrogel to be applied in wearable devices to detect glucose in biological fluids. This platform was proven to be suitable also for the highly sensitive detection of biological fluids with a low glucose concentration (Kajisa, T & Sakata, T. (2017) Glucose-responsive hydrogel electrode for biocompatible glucose transistor, Science and Technology of Advanced Materials, 18:1, 26-33, DOI: 10.1080/14686996.2016.1257344).
Matsumoto et al., 2009 prepared a totally synthetic, phenylboronic acid-based glucose-sensing FET. Phenylboronic acid was chemically introduced onto the FET gate surface in the form of a thin copolymer gel layer (Current Applied Physics, 9, Supplement, 2009, Pages e214-e217).
Another study describes a device that was developed for precise, controlled and painless extraction of blood dosage and was integrated with an electrolyte-monitoring system consisting of Ion Selective Field Effect Transistor (ISFET) for measuring the concentration level of Na+, K+ and Cl− in blood. The sensing elements comprised 5×5 mm2 area of PMMA hollow microneedles fabricated by Synchrotron Radiation deep X-ray lithography (S. Khumpuang et al., “Development of Bio-chemical Sensor System Integrated with Blood Extraction Device,” 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, 2007. pp. 847-850)
There remains an unmet need for a FET-biosensor design with a stable MNs structure which should be stretchable and have high skin conformability, while also being rigid and strong, in order to allow MNs penetration of the outer skin layer. Such wearable device should allow accurate measurement of extremely low concentrations of various biomarkers found in the interstitial fluid via a painless test, which can inter alia be employed for long term health monitoring.
The present disclosure provides a wearable device for real-time detection of biomarkers from interstitial fluids. According to some examples, the biosensor device is stretchable, stable, biocompatible, and minimally invasive and allows continuous detection and monitoring of various constituents of interstitial fluids that are important for subject's health and well-being. The device is based on a new architecture of field effect transistors (FETs) which sensing part relies on an extended gate (EG) comprising microneedles (MNs) that penetrate the skin to reach the ISF to measure biomarkers found therein. In this manner, the sensing part comprising MNs could be separated from the FET transducer part, which keeps the biosensor operating in a clean, noise-free environment with high sensitivity and stability. Furthermore, integration of the microneedles obviates the need for painful extraction methods and devices and allows real time in-vivo monitoring. Comparing with a flat extended gate FET sensor, the MNs-based extended gate field effect transistor (EGFET) according to the principles of the present disclosure showed ultra-sensitivity and 108 times-higher response amplitude when applied onto dry skin surface. Additional advantageous feature of the EGFET device is that the sensing part can be disposed after use and the FET can be combined with a new extended gate. The present disclosure further provides a fast, easy and inexpensive method of fabrication of the EGFET biosensor, and in particular, of its sensing part.
The sensing part (i.e., the extended gate) of the biosensor is based on microneedles which are supported on a stretchable and flexible substrate, which conforms to skin and can be thus worn comfortably for long periods of time. The inventors of the present disclosure have surprisingly discovered that implementing a thickness-gradient strategy to combine a rigid array of microneedles and a soft stretchable substrate conveniently solved the long-standing problem of using MNs in wearable devices. In particular, the higher thickness of the substrate in a portion of the substrate on which the MNs are disposed provided the required rigidity, which protected the MNs from deformation and breaking and the lower thickness of the remaining patch afforded for the comfortable attachment to the skin and also provided electrical connection to the FET. The MNs were connected to the FET via a specially designed electric contact in a form of an elongated electrode disposed on the lower-thickness partition of the substrate, which maintained its high conductivity under bending, twisting, and stretching of the substrate. Accordingly, the microneedle-loaded stiffness-tunable patch was shown to have good stretchability, flexibility, and conductivity at the same time. The EGFET device according to the principles of the present disclosure can be used in various continuous and online health monitoring applications where conformal MNs are required, such as, but not limited to, monitoring concentrations of various body electrolytes, sugars, and hormones.
According to one aspect, there is provided a wearable extended gate field effect transistor (EGFET) device for detecting and/or measuring a constituent of an interstitial fluid from the skin of a subject, the device comprising a polymeric substrate for fixing to the skin of the subject; an extended gate electrode comprising a first microneedle configured for accessing the interstitial fluid and a first electric contact, wherein the first microneedle and the first electric contact are disposed on the polymeric substrate; and a field effect transistor (FET) associated with the first microneedle through the first electric contact.
According to some examples, the device further comprising a reference electrode comprising a second microneedle and a second electric contact, wherein the second microneedle and the second electric contact are disposed on the polymeric substrate.
According to some examples, the polymeric substrate has a thickness gradient between the first microneedle and the first electric contact.
According to some examples, the thickness of a portion of the polymeric substrate on which the first microneedle is disposed is at least 50% higher than the thickness of a portion of the polymeric substrate on which the first electric contact is disposed.
According to some examples, the portion of the polymeric substrate on which the first microneedle is disposed is substantially rigid. According to some examples, the portion of the polymeric substrate on which the second microneedle is disposed is substantially rigid.
According to some examples, the portion of the polymeric substrate on which the first electric contact is disposed is substantially flexible and stretchable. According to some examples, the portion of the polymeric substrate on which the second electric contact is disposed is substantially flexible and stretchable.
According to some examples, the portion of the polymeric substrate on which the first microneedle is disposed and the portion of the polymeric substrate on which the first electric contact is disposed are made of the same polymeric material. According to some examples, the portion of the polymeric substrate on which the second microneedle is disposed and the portion of the polymeric substrate on which the second electric contact is disposed are made of the same polymeric material.
According to some examples, the polymeric substrate is made of a polymeric material selected from the group consisting of styrene-block-isoprene-block styrene (SIS), 1-styrene-butadiene-styrene block copolymer (SBS), 2-styrene ethylene butylene styrene block copolymer (SEBS), polydimethylsiloxane (PDMS), polybutadiene rubber, polyurethane thermoplastic elastomer, low-density polyethylene (LDPH), polyisoprene, chloroprene rubber (CR), silicone rubber, and combinations and derivatives thereof. Each possibility represents a separate example of the disclosure. In some exemplary examples, the polymeric substrate is made of styrene-block-isoprene-block styrene (SIS).
According to some examples, the first microneedle, the second microneedle or both are made of a material selected from the group consisting of a polymer, metal, metal alloy, carbon, and combinations thereof. Each possibility represents a separate example of the disclosure.
In some examples, the metal is selected from the group consisting of Au, Ag, Pt, Ni, Ti, Cr, Cu, Pd, Al, combinations, and alloys thereof. Each possibility represents a separate example of the disclosure.
In some examples, the polymer is selected from the group consisting of polyester, polystyrene, polycarbonate, poly (methyl methacrylate), acrylate, polyvinylpyrrolidone, epoxy-based negative photoresist, and combinations thereof. Each possibility represents a separate example of the disclosure.
In some exemplary examples, the first microneedle is made of polystyrene, coated by a metal selected from Au, Pt, and Ni. Each possibility represents a separate example of the disclosure.
According to some examples, the first microneedle is modified with a biorecognition element selected from the group consisting of an enzyme, antibody, aptamer, ion-selective membrane (ISM), protonically doped polymer, DNA, ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), molecularly imprinted polymer (MIP), and combinations thereof. Each possibility represents a separate example of the disclosure.
In some examples, the biorecognition element is bound to the first microneedle via a linker or is held within a supporting film or matrix.
In some exemplary examples, the biomarker is a sodium ion and the biorecognition element comprises Na ionophore X and sodium tetrakis [3,5-bis(trifluoromethyl)phenyl] borate (Na-TFPB). In some related examples, the biorecognition material is immobilized on the first microneedle by polyvinyl chloride (PVC) and bis(2-ethylehexyl) sebacate (DOS).
In some exemplary examples, the biomarker is cortisol and the biorecognition element comprises monoclonal anti-cortisol. In some related examples, the biorecognition element is immobilized on the first microneedle via a (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde linker.
In some exemplary examples, the biomarker is glucose and the biorecognition element is glucose oxidase. In some related examples, the biorecognition element is immobilized on the first microneedle by chitosan. In further examples, chitosan is mixed with carbon nanotubes.
In some exemplary examples, the biomarker is a hydronium ion and the biorecognition element is polyaniline (PANI).
According to some examples, the second microneedle is made of polystyrene coated by silver.
According to some examples, the second microneedle is modified with a metal material configured to apply voltage on the surface of the second microneedle. In certain examples, the material is selected from the group consisting of AgCl/NaCl and Au. Each possibility represents a separate example of the disclosure.
According to some examples, the gate voltage of the FET is responsive to the metal material.
According to some examples, the first microneedle, the second microneedle or both have a conical shape having a height between about 250 μm and about 5 mm and a diameter at its base between about 100 μm and 2.5 mm.
According to some examples, the extended gate electrode comprises a plurality of first microneedles disposed on the polymeric substrate and arranged in an array, wherein the FET is associated with the plurality of first microneedles through the first electric contact. According to some examples, the reference electrode comprises a plurality of second microneedles disposed on the polymeric substrate and arranged in an array.
According to some examples, the first electric contact, the second electric contact or both comprise electrically conductive elongated nanostructures. In further examples, the electrically conductive elongated nanostructures are selected from the group consisting of nanotubes, nanowires, nanoribbons, nano-whiskers, nanostrips, nanorods, and combinations thereof. Each possibility represents a separate example of the disclosure. In yet further examples, the electrically conductive elongated nanostructures are made of a material selected from the group consisting of a metal, metal alloy, carbon, and combinations thereof. Each possibility represents a separate example of the disclosure. In some exemplary examples, the first electric contact and the second electric contact comprises silver nanowires (AgNWs).
According to some examples, the FET is selected from the group consisting of a metal-oxide-semiconductor field-effect transistor (MOSFET), junctionless nanowire transistor (JLNT), metal-nitride-oxide-semiconductor transistor (MNOS), junction field-effect transistor (JFET), static induction transistor (SIT); heterostructure insulated-gate field-effect transistor (HIGFET), modulation-doped field-effect transistor (MODFET); tunnel field-effect transistor (TFET), high-electron-mobility transistor (HEMT), metal-semiconductor field-effect transistor (MESFET), nanoparticle organic memory field-effect transistor (NOMFET), graphene nanoribbon field-effect transistor (GNRFET), vertical-slit field-effect transistor (VeSFET), carbon nanotube field-effect transistor (CNTFET), organic field-effect transistor (OFET), quantum field effect transistor (QFET), Schottky-barrier field-effect transistor (SB-FET), and graphene-based field effect transistor (GFET). Each possibility represents a separate example of the disclosure. In some exemplary examples, the FET is a MOSFET.
According to another aspect, there is provided a system for detecting and/or measuring a constituent of an interstitial fluid from the skin of a subject, the system comprising: the wearable EGFET device according to the aspect and various examples hereinabove; and at least one of a control unit being in electrical communication with the EGFET device, which measures an electrical signal generated by the FET in response to an interaction between the first microneedle and said constituent; and a transmitter, which receives the electrical signal generated by the FET in response to the interaction between the first microneedle and said constituent and transmits said signal to a remote server and/or to a portable electronic device.
According to some examples, the control unit is in electrical communication with each one of the FET, extended gate electrode, and reference electrode within the EGFET device.
According to some examples, the system further comprises a display unit in electrical communication with the control unit for displaying information related to the measuring of the electrical signal, and/or means for determining concentration of the constituent in the interstitial fluid upon receipt of the electrical signal.
According to another aspect, there is provided a method for detecting and/or measuring a constituent of an interstitial fluid from the skin of a subject, the method comprising: (a) providing the system according to the aspect and various examples hereinabove; (b) fixing the wearable EGFET to the skin of a subject, thereby enabling an interaction between the first microneedle and said constituent; (c) measuring an electrical signal generated by the FET in response to the interaction between the first microneedle and said constituent; and (d) analyzing the electrical signal by at least one of the control unit, the remote server and the portable electronic device.
According to some examples, fixing the wearable EGFET to the skin of the subject comprises directly contacting the first microneedle with the skin of the subject, wherein the measuring step is performed while the wearable EGFET is fixed to the skin of the subject.
According to some examples, analyzing the electrical signal comprises comparing the electrical signal with a calibration curve and/or reference data.
According to some examples, the method further comprises displaying information related to the measuring of the electrical signal or a result of the step of analyzing (step (d)) onto a display unit being in electrical communication with the control unit, the remote server, or the portable electronic device.
According to another aspect, there is provided a method for fabricating the wearable EGFET device according to the aspect and various examples hereinabove, the method comprising: (a) providing the first microneedle, the first electric contact, and the polymeric substrate; (b) providing the FET; (c) connecting the first electric contact with the polymeric substrate; (d) connecting the first microneedle with the polymeric substrate; and (e) associating the FET with the first microneedle.
According to some examples, steps (a) and (b) can be performed in any order. According to some examples, steps (a) and (b) are performed simultaneously.
According to some examples, the method further comprises a step of forming the second microneedle and the second electric contact, and a step of connecting the second microneedle and the second electric contact with the polymeric substrate.
According to some examples, providing the first electric contact and/or and providing the second electric contact comprises spray-coating hydrophobic electrically conductive elongated nanostructures onto a Si wafer coated with a mask having a predefined opening, peeling the mask and annealing the obtained first electric contact and/or second electric contact.
According to some examples, providing the polymeric substrate comprises forming a first substantially flexible and stretchable polymeric film and combining said film with a second substantially flexible and stretchable polymeric film, which has been pre-stretched.
According to some examples, providing the polymeric substrate comprises thickening a portion of the polymeric substrate by applying a solution of the substantially flexible and stretchable polymer onto said portion.
According to some examples, the step of connecting the first electric contact with the polymeric substrate comprises spin-coating a solution of the substantially flexible and stretchable polymer onto the first electric contact to form the first substantially flexible and stretchable polymeric film prior to combining said first substantially flexible and stretchable polymeric film with the second substantially flexible and stretchable polymeric film, which has been pre-stretched.
According to some examples, the step of connecting the first microneedle with the polymeric substrate comprises fixing the first microneedle onto the thickened portion of the polymeric substrate.
Further examples and the full scope of applicability of the present disclosure will become apparent from the detailed description given hereinafter. However, it should be understood that the detailed description and specific examples, while indicating preferred examples of the disclosure, are given by way of illustration only, since various changes and modifications within the spirit and scope of the disclosure will become apparent to those skilled in the art from this detailed description.
Some of the examples of the disclosure are described herein with reference to the accompanying figures. The description, together with the figures, makes apparent to a person having ordinary skill in the art how some examples may be practiced. The figures are for the purpose of illustrative description and no attempt is made to show structural details of an example in more detail than is necessary for a fundamental understanding of the disclosure. For the sake of clarity, some objects depicted in the figures are not to scale.
In one aspect, there is provided a wearable extended gate field effect transistor (EGFET) device for detecting and/or measuring a constituent of an interstitial fluid from the skin of a subject, the device comprising: a substrate for fixing to the skin of the subject; an extended gate electrode comprising a first microneedle configured for accessing the interstitial fluid and a first electric contact, wherein the first microneedle and the first electric contact are disposed on the polymeric substrate; and a field effect transistor (FET) associated with the first microneedle through the first electric contact. The terms “wearable” and “skin-mountable”, which are used herein interchangeably, refer to a device which is configured to be placed or fixed upon the skin.
The term “interstitial fluid”, as used herein, refers to the fluid that fills the spaces between cells, the fluid being composed of water, amino acids, sugars, fatty acids, coenzymes, hormones, neurotransmitters, salts, and cellular products.
The terms “constituent of an interstitial fluid” and “biomarker”, which are used herein interchangeably, refer to any constituent of the interstitial fluid, which can be detected via changes of surface potential of a measuring device and/or which monitoring is of interest for evaluating health or wellbeing of a subject. Non-limiting examples of biomarkers, which can be detected by the EGFET device according to the principles of the present disclosure include glucose, cortisol, lactate, alcohol, pH, enzymes, sodium (Na+) and other electrolytes such as K+, Ca2+ and Mg2+.
The term “associated”, as used herein, refers in some examples, to an electrical connection between two or more elements. In further examples, said term refers to an electrical connection, while said two or more elements are spatially separated. In some currently preferred examples, the transducer is not disposed on the polymeric substrate.
According to some examples, the device further comprises a reference electrode comprising a second microneedle and a second electric contact, wherein the second microneedle and the second electric contact are disposed on the substrate.
According to some examples, the portion of the substrate on which the first electric contact is disposed is stretchable.
The polymeric substrate comprising the first microneedle, the second microneedle, the first electric contact and the second electric contact are also termed herein “microneedle (MN) patch”.
According to some examples, the substrate is a polymeric substrate. The term “polymeric substrate”, as used herein, refers to a solid or semi-solid support having a surface formed of a polymer or a solid support which is formed entirely of a polymer. The term “polymer” as used herein refers generally to a macromolecule composed of repeating monomer units, and is meant to encompass natural, synthetic, and semi-synthetic polymers. The term “polymeric substrate” is also meant to encompass composite polymeric materials, such as, but not limited to, polymeric materials having fillers, plasticizers, and/or fibers therein.
In some examples, the polymeric substrate is formed entirely of a polymer.
According to some examples, the polymeric substrate has a thickness gradient between the first microneedle and the first electric contact.
According to some examples, the polymeric substrate has a thickness gradient between the second microneedle and the second electric contact.
According to some examples, the thickness of a portion of the polymeric substrate on which the first microneedle is disposed is at least about 50% higher than the thickness of a portion of the polymeric substrate on which the first electric contact is disposed. In further examples, the thickness of a portion of the polymeric substrate on which the first microneedle is disposed is at least about 100%, about 200%, about 300%, about 400%, about 500% higher than the thickness of a portion of the polymeric substrate on which the first electric contact is disposed.
According to some examples, the thickness of a portion of the polymeric substrate on which the second microneedle is disposed is at least about 50% higher than the thickness of a portion of the polymeric substrate on which the second electric contact is disposed. In further examples, the thickness of a portion of the polymeric substrate on which the second microneedle is disposed is at least about 100%, about 200%, about 300%, about 400%, about 500% higher than the thickness of a portion of the polymeric substrate on which the second electric contact is disposed. According to some examples, the portion of the polymeric substrate on which the first microneedle is disposed is substantially rigid. According to some examples, the portion of the polymeric substrate on which the second microneedle is disposed is substantially rigid.
The term “substantially rigid”, as used herein, refers, in some examples, to the ability of a material to resist deformation, e.g., elastic, plastic, or otherwise, wherein said resistance is adequate for the proper functioning of the microneedles, when the EGFET device is fixed to skin.
According to some examples, the portion of the polymeric substrate on which the first electric contact is disposed is substantially flexible and stretchable. According to some examples, the portion of the polymeric substrate on which the second electric contact is disposed is substantially flexible and stretchable.
The term “substantially flexible”, as used herein, refers to the ability of a material to elastically deform in response to applied pressure or strain, wherein said deformation is proportional to the amount of applied pressure or strain.
The term “substantially stretchable”, as used herein, refers to the ability of a material to allow a substantial increase in its length, width, and/or height in at least one spatial direction without loss of structural integrity. A substantial increase is understood to include an increase by at least 10% and may include an increase of 50%, 100%, or more.
According to some examples, the portion of the polymeric substrate on which the first microneedle is disposed and the portion of the polymeric substrate on which the first electric contact is disposed are made of the same polymeric material. According to some examples, the portion of the polymeric substrate on which the second microneedle is disposed and the portion of the polymeric substrate on which the second electric contact is disposed are made of the same polymeric material.
According to some examples, the polymeric substrate is made of a polymeric material selected from the group consisting of styrene-block-isoprene-block styrene (SIS), 1-styrene-butadiene-styrene block copolymer (SBS), 2-styrene ethylene butylene styrene block copolymer (SEBS), polydimethylsiloxane (PDMS), polybutadiene rubber, polyurethane thermoplastic elastomer, low-density polyethylene (LDPH), polyisoprene, chloroprene rubber (CR), silicone rubber, and combinations and derivatives thereof.
The term “derivative”, as used herein, refers to a compound that has been subjected to one or more chemical modifications, preferably while maintaining the majority of its functionalities and structural features. Such chemical modifications include, for example, substitution, oxidation, reduction, and the like.
According to some examples, the polymeric substrate is composed of at least two layers, comprising a layer of a pre-stretched polymer and a layer of a pristine polymer (i.e., not pre-stretched). According to some examples, the polymeric substrate is composed of at least three layers, comprising a layer of a pre-stretched polymer; a layer of a pristine polymer; and an additional layer of the pristine polymer, which is located within the portion of the polymeric substrate on which the first microneedle is disposed.
According to some examples, the first microneedle, the second microneedle or both are made of a material selected from the group consisting of a polymer, metal, metal alloy, carbon, and combinations thereof. Non-limiting examples of suitable metals include Au, Ag, Pt, Ni, Ti, Cr, Cu, Pd, Al, combinations, and alloys thereof. Non-limiting examples of suitable polymers include polyester, polystyrene, polycarbonate, poly (methyl methacrylate), acrylate, polyvinylpyrrolidone, epoxy-based negative photoresist, and combinations thereof. Non-limiting examples of suitable carbons include graphite, graphene, graphene oxide, carbon nanotubes, carbon nanofibers, carbon black, fullerenes, and combinations thereof.
According to some examples, the first microneedle, the second microneedle or both are made of an insulating material coated with an electrically conductive material.
In some examples, the first microneedle is made of an Au-coated polymer, Pt-coated polymer, or Ni-coated polymer. In some exemplary examples, the first microneedle is made of polystyrene coated by a metal selected from Au, Pt, and Ni.
In some currently preferred examples, the first microneedle is modified with a biorecognition element.
The term “biorecognition element”, as used herein, refers to a compound, which is selective to the target constituent (or biomarker) in the interstitial fluid. The biorecognition element can be, for example, a receptor or a probe molecule.
According to some examples, the biorecognition element is selected from the group consisting of an enzyme, antibody, aptamer, ion-selective membrane (ISM), protonically doped polymer, DNA, ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), molecularly imprinted polymer (MIP), and combinations thereof.
The biorecognition element can be bound to the first microneedle via a linker. In some examples, the biorecognition element is held within a supporting film or matrix.
In some exemplary examples, the biomarker is a sodium ion and the biorecognition element comprises Na ionophore X and sodium tetrakis [3,5-bis(trifluoromethyl)phenyl] borate (Na-TFPB). In some related examples, the biorecognition material is immobilized on the first microneedle by polyvinyl chloride (PVC) and bis(2-ethylehexyl) sebacate (DOS).
In some exemplary examples, the biomarker is cortisol and the biorecognition element comprises monoclonal anti-cortisol. In some related examples, the biorecognition element is immobilized on the first microneedle via a (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde linker.
In some exemplary examples, the biomarker is glucose and the biorecognition element is glucose oxidase. In some related examples, the biorecognition element is immobilized on the first microneedle by chitosan. In further examples, chitosan is mixed with carbon nanotubes.
In some exemplary examples, the biomarker is a hydronium ion and the biorecognition element is polyaniline (PANI).
According to some examples, the second microneedle is made of polystyrene coated by silver.
According to some examples, the second microneedle is modified with a material configured to maintain a constant electrical potential on the surface of the second microneedle. According to further examples, the material is selected from the group consisting of AgCl/NaCl and Au.
According to some examples, the first microneedle has a conical shape having a height between about 250 μm and about 5 mm and a diameter at its base between about 100 μm and 2.5 mm.
According to some examples, the second microneedle has a conical shape having a height between about 250 μm and about 5 mm and a diameter at its base between about 100 μm and 2.5 mm
According to some examples, the first microneedle comprises a plurality of first microneedles disposed on the polymeric substrate. In further examples, the FET is associated with the plurality of first microneedles through the first electric contact. In certain examples, the plurality of first microneedles are arranged in an array. In some exemplary examples, the plurality of first microneedles are arranged in a 3×3 array.
According to some examples, the plurality of first microneedles are electrically connected therebetween. According to further examples, the plurality of first microneedles are disposed on an electrically conducting layer disposed on the polymeric substrate.
According to some examples, the second microneedle comprises a plurality of second microneedles disposed on the polymeric substrate. In certain examples, the plurality of second microneedles are arranged in an array. In some exemplary examples, the plurality of second microneedles are arranged in a 3×3 array.
According to some examples, the plurality of second microneedles are electrically connected therebetween. According to further examples, the plurality of second microneedles are disposed on an electrically conducting layer disposed on the polymeric substrate.
According to some examples, the first electric contact, the second electric contact or both comprise electrically conductive elongated nanostructures. According to further examples, the electrically conductive elongated nanostructures are selected from the group consisting of nanotubes, nanowires, nanoribbons, nano-whiskers, nanostrips, nanorods, and combinations thereof. In yet further examples, the elongated nanostructures are made of a material selected from the group consisting of a metal, metal alloy, carbon, and combinations thereof.
In some exemplary examples, the first electric contact and the second electric contact comprises silver nanowires (AgNWs).
According to some examples, the first electric contact is elongated, being at least about 10 times longer than the diameter of the first microneedle at its base.
According to some examples, the second electric contact is elongated, being at least about 10 times longer than the diameter of the second microneedle at its base. As explained hereinabove, the extended gate electrode is configured to sense the target biomarker. When the biomarker interacts with the first microneedle, wherein said interaction involves electron transfer, or wherein said biomarker is charged, said interaction changes the surface potential of the extended gate, which, while not being physically connected with a transducer, is electrically connected therewith. In some currently preferred examples, the transducer is a FET. In certain such examples, the change in the charge distribution of the extended gate can change the charge distribution of the semiconductor of the FET, resulting in a change in conductance of the FET channel, thereby providing detection of the biomarker, wherein the extent of the change in conductance can be used to assess the concentration of the biomarker. According to some examples, the FET is selected from the group consisting of a metal-oxide-semiconductor field-effect transistor (MOSFET), junctionless nanowire transistor (JLNT), metal-nitride-oxide-semiconductor transistor (MNOS), junction field-effect transistor (JFET), static induction transistor (SIT); heterostructure insulated-gate field-effect transistor (HIGFET), modulation-doped field-effect transistor (MODFET); tunnel field-effect transistor (TFET), high-electron-mobility transistor (HEMT), metal-semiconductor field-effect transistor (MESFET), nanoparticle organic memory field-effect transistor (NOMFET), graphene nanoribbon field-effect transistor (GNRFET), vertical-slit field-effect transistor (VeSFET), carbon nanotube field-effect transistor (CNTFET), organic field-effect transistor (OFET), quantum field effect transistor (QFET), Schottky-barrier field-effect transistor (SB-FET), and graphene-based field effect transistor (GFET).
According to some exemplary examples, the FET is a MOSFET.
According to some examples, the extended gate electrode and/or the reference electrode are disposable and the FET is reusable.
According to another aspect, there is provided a system for detecting and/or measuring a constituent of an interstitial fluid from the skin of a subject, the system comprising: the wearable EGFET device according to the aspect and various examples hereinabove; and at least one of a control unit being in electrical communication with the EGFET device, which measures an electrical signal generated by the FET in response to an interaction between the first microneedle and said constituent; and a transmitter, which receives the electrical signal generated by the FET in response to the interaction between the first microneedle and said constituent and transmits said signal to a remote server and/or to a portable electronic device.
The term “interaction”, as used herein, refers to either a physical contact, such as, e.g., binding, or a chemical reaction taking place between the first microneedle and the biomarker, such as, e.g., a redox reaction.
According to some examples, the control unit is in electrical communication with each one of the FET, extended gate electrode, and reference electrode within the EGFET device.
According to some examples, the system further comprises a display unit in electrical communication with the control unit for displaying information related to the measuring of the electrical signal.
According to some examples, the system further comprises means for determining concentration of the constituent in the interstitial fluid upon receipt of the electrical signal. Said means can be a processing unit or a computer.
In another aspect, there is provided a method for detecting and/or measuring a constituent of an interstitial fluid from the skin of a subject, the method comprising: (a) providing the system according to the aspect and various examples hereinabove; (b) fixing the wearable EGFET to the skin of a subject, thereby enabling an interaction between the first microneedle and said constituent; (c) measuring an electrical signal generated by the FET in response to the interaction between the first microneedle and said constituent; and (d) analyzing the electrical signal by at least one of the control unit, the remote server and the portable electronic device.
According to some examples fixing the wearable EGFET to the skin of the subject comprises directly contacting the first microneedle with the skin of the subject.
According to some examples, analyzing the electrical signal comprises comparing the electrical signal with a calibration curve and/or reference data.
According to some examples, the method further comprises displaying information related to the measuring of the electrical signal or a result of the step of analyzing (step (d)) onto a display unit in electrical communication with the control unit, the remote server, or the portable electronic device.
According to some examples, the measuring step is performed while the wearable EGFET is fixed to the skin of the subject, the method thereby providing real-time detection and/or measurement of the constituent of the interstitial fluid.
In some exemplary examples, the biomarker is a sodium ion and the biorecognition element comprises Na ionophore X and sodium tetrakis [3,5-bis(trifluoromethyl)phenyl] borate (Na-TFPB). In some related examples, the biorecognition material is immobilized on the first microneedle by polyvinyl chloride (PVC) and bis(2-ethylehexyl) sebacate (DOS).
In some exemplary examples, the biomarker is cortisol and the biorecognition element comprises monoclonal anti-cortisol. In some related examples, the biorecognition element is immobilized on the first microneedle via a (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde linker.
In some exemplary examples, the biomarker is glucose and the biorecognition element is glucose oxidase. In some related examples, the biorecognition element is immobilized on the first microneedle by chitosan. In further examples, chitosan is mixed with carbon nanotubes.
In some exemplary examples, the biomarker is a hydronium ion and the biorecognition element is polyaniline (PANI).
In another aspect, there is provided a method for fabricating the wearable EGFET device according to the aspect and various examples hereinabove, the method comprising: (a) providing the first microneedle, the first electric contact, and the substrate; (b) providing the FET; (c) connecting the first electric contact with the substrate; (d) connecting the first microneedle with the substrate; and (e) associating the FET with the first microneedle.
According to some examples, step (a) comprises forming the first microneedle; forming the first electric contact; and forming the substrate. According to some examples, said steps can be performed in any order. According to some examples, at least two of said steps are performed simultaneously.
According to some examples, steps (a) and (b) can be performed in any order. According to some examples, steps (a) and (b) are performed simultaneously.
According to some examples, the method further comprises a step of forming the second microneedle. In some related examples, the method further comprises a step of forming the second electric contact. In further related examples, the method further comprises a step of connecting the second microneedle and the second electric contact with the substrate.
According to some examples, the step of forming the first microneedle comprises forming a mold comprising conical holes and filling said holes with a biocompatible polymer. In some related examples, the step of forming the first microneedle further comprises coating the obtained first microneedle with a metal. The metal can be selected from the group consisting of Au, Ag, Pt, Ni, Ti, Cr, Cu, Pd, Al, combinations, and alloys thereof. In further examples, the step of forming the first microneedle further comprises modifying the obtained first microneedle with a biorecognition element.
According to some examples, the step of forming the second microneedle comprises forming a mold comprising conical holes and filling said holes with a biocompatible polymer. In some related examples, the step of forming the second microneedle further comprises coating the obtained second microneedle with a metal selected from the group consisting of Au, Ag. Pt, Ni. Ti, Cr, Cu, Pd, Al, combinations, and alloys thereof. In further examples, the step of forming the second microneedle further comprises modifying the obtained second microneedle with a material configured to maintain a constant potential on the surface of the second microneedle.
According to some examples, the step of forming the first electric contact and/or the step of forming the second electric contact comprise spray-coating hydrophobic electrically conductive elongated nanostructures onto a Si wafer coated with a mask having a predefined opening, peeling the mask and annealing the obtained electric contact.
According to some examples, the substrate is a polymeric substrate. According to some examples, the step of forming the polymeric substrate comprises forming a first substantially flexible and stretchable polymeric film and combining said film with a second substantially flexible and stretchable polymeric film, which has been pre-stretched.
According to some examples, the step of forming the polymeric substrate comprises thickening a portion of the obtained polymeric substrate. According to some related examples, said thickening comprises applying a solution of the substantially flexible and stretchable polymer onto said portion.
According to some examples, the step of connecting the first electric contact with the polymeric substrate comprises spin-coating a solution of the substantially flexible and stretchable polymer onto the first electric contact to form the first substantially flexible and stretchable polymeric film prior to combining said first substantially flexible and stretchable polymeric film with the second substantially flexible and stretchable polymeric film, which has been pre-stretched. According to some examples, the polymeric film is at least about 50% pre-stretched. According to further examples, the polymeric film is at least about 100% pre-stretched.
According to some examples, the step of connecting the second electric contact with the polymeric substrate comprises spin-coating a solution of the substantially flexible and stretchable polymer onto the second electric contact to form the first substantially flexible and stretchable polymeric film prior to combining said first substantially flexible and stretchable polymeric film with the second substantially flexible and stretchable polymeric film, which has been pre-stretched.
According to some examples, the step of connecting the first microneedle with the polymeric substrate comprises fixing the first microneedle onto the thickened portion of the polymeric substrate. According to some examples, the step of connecting the second microneedle with the polymeric substrate comprises fixing the second microneedle onto the thickened portion of the polymeric substrate.
According to some examples, the step of associating the FET with the first microneedle comprises electrically connecting the FET to the first electric contact.
As used herein and in the appended claims the singular forms “a”, “an,” and “the” include plural references unless the content clearly dictates otherwise. Thus, for example, reference to “a microneedle” includes a plurality of such microneedles as known to those skilled in the art, and so forth. It should be noted that the term “and” or the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
As used herein, the term “about”, when referring to a measurable value such as an amount, a temporal duration, and the like, is meant to encompass variations of +/−10%, more preferably +/−5%, even more preferably +/−1%, and still more preferably +/−0.1% from the specified value, as such variations are appropriate to perform the disclosed methods.
The following examples are presented in order to more fully illustrate some examples of the disclosure. They should, in no way be construed, however, as limiting the broad scope of the disclosure. One skilled in the art can readily devise many variations and modifications of the principles disclosed herein without departing from the scope of the disclosure.
A scheme of the stretchable skin-conformal microneedle-based extended gate Field-Effect Transistor (MNs-EGFET) device and its measurement system is demonstrated in
In some examples, as illustrated in
The overall fabrication process of the MNs is demonstrated in
In this design, the exceptional architecture of the MNs enables accessibility to the interstitial fluids' (ISF's) sodium and controllability of the FET's mode of operation in real-time, without the need for extraction methods or any excessive activities. Subsequently, the concentration input is transduced to an electric signal by the FET and the output can be directly transferred to doctors and clinics via smartphone/computer integrated with IoT technologies (
For the synthesis of silver nanowires (AgNWs), 2.5 g of Polyvinylpyrrolidone (PVP) was added to 40 mL ethyl glycol solution and mixed with 100 μL of 0.15 M FeCl3 in ethyl glycol for 5 min at 160° C. 100 μL of 0.15M NaCl was added as a catalyst for the reaction, and then 10 mL of 1.5 M AgNO3 was added dropwise while mixing until a color change of the mixture to light silver is preserved. Methanol was added after 2 h to stop the reaction. To obtain the AgNWs, the samples are then centrifuged to separate from the other residues. Pure AgNWs were then modified with 1H, 1H,2H,2H-Perfluorodecanethiol, producing hydrophobic AgNWs-Fluorine (AgNWs-F). In brief, 50 mg AgNWs were suspended in 5 mL ethanol/chloroform (1:1 by volume) solution and sonicated for 5 min. 5 μL of 1H,1H,2H,2H-perfluorodecanethiol was added and the mixture was sonicated for 10 min, after which it was kept stirred overnight at 90° C. The mixture was then centrifuged 3 more times with ethanol/chloroform solution (3,000 rpm for 6 min) to separate the residues and dried in a vacuum oven at 50° C.
To fabricate the rigid-soft gate structure, an electrode mask was applied on a hydrophobic Si wafer and AgNWs-F were spray-coated. After peeling off the mask, the sprayed AgNWs-F were annealed at 200° C. for 20 min to obtain a conductive AgNWs electrode. 100 mg/mL styrene-block-isoprene-block styrene (SIS) in toluene was spin-coated onto AgNWs electrode with 1,000 rpm for 60 s to fabricate stretchable thin film. This thin film was transferred onto another 100% pre-stretched SIS film prepared with Teflon template to obtain a stretchable AgNWs electrode (the soft part). To create a variant thickness substance, SIS solution was drop-casted at specific points onto the backside of a prepared stretchable AgNWs electrode to thicken an area on the film (the rigid part). The rigid MNs were then fixed on the rigid part of this thickness-gradient AgNWs electrode film using silver paint. The MNs array (the gate) integrated with the SIS substrate was then combined with the transducer device (FET TO-236) to obtain the MN-EGFET platform (
SEM image of the sprayed AgNWs electrodes demonstrates their network structure (
The stretchability of Styrene-block-isoprene-block styrene (SIS) polymer was investigated using a tensile test simulation as shown in
Na+ MN-EGFET biosensor was fabricated by modifying the MNs with sodium-selective membrane. For this purpose, 10 mg sodium ionophore X were mixed with 5.5 mg sodium tetrakis [3,5-bis(trifluoromethyl)phenyl] borate (Na-TFPB), 33 mg polyvinyl chloride (PVC) and 654.5 mg bis(2-ethylehexyl) sebacate (DOS) and dissolved in 6.6 mL tetrahydrofuran (THF). The solution was stored at 4° C. overnight and then 5 μL was drop-casted onto the extended gate electrode.
To test its flexibility on skin surface, the Na+ MN-EGFET biosensor patch was placed on a dry skin and presented high skin conformability (
In order to evaluate the sensing performance of Na+ MN-EGFET, sodium solutions at a concentration range of from 10 to 160 mM were made, given that the normal average concentration of the Na+ in ISF is ˜145 mM. The electrical behavior of the fabricated EGFET sensor was measured using Keithley 2536A System Source meter. As shown in
A repeatability test was conducted by applying 3 cycles of elevated sodium concentrations (10 mM and 160 mM), leading to a repeatable elevation of the drain-source current response (
The selectivity of the Na+ MN-EGFET was also assessed by applying sodium and other analytes, including K+, Ca2+, Mg2+, glucose and cortisol. As shown in
The sensing performance of different metal electrode-based MN-EGFET Na+ sensors by fabricating Na+ biosensors using different metals such as Nickel (Ni) and Platinum (Pt) was examined. As is shown in
The sensing performance of a flat EGFET sensor was measured as described in Example 1 and compared with the sensing performance of the MN-EGFET sensor. As is shown in
Electric potential simulation was conducted via COMSOL. Modifying the MNs with a functional molecular monolayer (e.g., ISM) and introducing a charged analyte (e.g., Na+), lead to a charged interface. The surface electric potential (V) caused by the adsorbed charged analytes, was simulated for both MNs and flat electrodes, in this case the electric potential distribution represents the positive charged sodium molecules (Na+). As presented in
A real-time on-body trial was performed to compare between the MNs and flat sensor patches (
In order to test applicability of the MN-EGFET device in the detection of other types of biomarkers, three additional sensors, modified by different biorecognition elements were prepared and examined, said biorecognition elements being: polyaniline (PANI); glucose oxidase; and anti-cortisol antibodies.
pH sensor was fabricated by the deposition of polyaniline (PANI) by cyclic voltammetry (CV) versus SCE electrode from −0.5V to 1.5V for 40 cycles with a scan rate of 100 mV/sec. 0.1 M aniline in HCl was used as deposition solution.
Glucose sensor was fabricated by modifying the Au gate electrode with glucose oxidase (Gox). For this purpose, chitosan was dissolved in 2% (v/v) acetic acid solution by stirring for 1 h to prepare 1% (w/v) chitosan solution; 1 mL of the solution was then mixed with 2 mg of carbon nanotubes and ultrasonicated for 30 min. 30 mg/mL GOx was mixed well with the Chitosan/CNTs solution at a 2:1 (v/v) ratio. A deposition of a mediator layer of Prussian blue on an Au gate was then made by CV versus SCE electrode from −0.2V to 1V for 3 cycles with a scan rate of 100 mV/sec. The solution of GOx/Chitosan/CNTs was then drop-casted on the Prussian blue Au gate (5 μl) to get the glucose sensor, which was kept at overnight at 4° C. until use.
Cortisol sensor was fabricated by modifying the Au gate electrode with anti-cortisol antibodies. For this purpose, The Au gate electrode was first cleaned using ethanol and then with pure water before being thoroughly dried. Immobilization of the monoclonal anti-cortisol was accomplished on the gate by the APTES-GA method. First, the electrode was immersed in 50 mM solution of 6-mercapto −1-hexanol (MCH) in ethanol overnight to get an electrode surface with OH terminals, due to the fact that thiol groups come together to create a self-assembled monolayer (SAM) on the surface of Au. The electrode was washed with ethanol and purified water. It was then immersed in 5% (3-aminopropyl)triethoxysilane (APTES) in a solution of 95% ethanol for 2 h and cleaned with ethanol and water. 2.5% glutaraldehyde (GA) was applied as a cross-linker to the electrode for 1 h and then washed with water. The antibodies (1 mg/mL in Tris-HCl Buffer, pH 8) were applied for 2 h. Finally, 100 mM ethanol amine (EA) was added to block the free sites and prevent the nonspecific binding of other molecules.
The sensing performance of each sensor was separately characterized using a Keithley analyzer with corresponding analyte solutions. Furthermore, the effect of confounding biomarkers on the MN-EGFET performance was tested using the sodium ion sensor, the pH sensor, the glucose sensor and the cortisol sensor.
PANI exhibited a unique electrical property in which the resistance can be reversibly changed through the protonation and deprotonation process by acid/base. After coating PANI onto the EG, the MN-EGFET platform had a remarkable sensing performance for pH, with 0.85 mA/pH sensitivity (
The representative current responses of MN-EGFETs coated with glucose oxidase (
The biocompatibility of the MNs patch for sodium sensing was systematically evaluated both in vitro and in vivo.
To check the in vitro biocompatibility of the MNs patch, mouse pre-osteoblast MC3T3-E1 cells (CRL-2594) and murine fibroblast NIH3T3 cells (CRL-1658), obtained from American Type Culture Collection (ATCC, USA), were cultured with or without the MNs patch for 24 h. MC3T3-E1 cells were cultured in alpha-MEM culture media supplemented with 10% FBS and 1% penicillin-streptomycin at 37° C. with incubation in air plus 5% CO2. NIH3T3 cells were cultured in DMEM culture media supplemented with 10% FBS and 1% penicillin-streptomycin at 37° C. with incubation in air plus 5% CO2.
Cell viability assay, Live/Dead staining, apoptosis assay and reactive oxygen species (ROS) assay for cells cultured with or without MNs patch were carried out separately.
For Cell viability assay, MC3T3-E1 and NIH3T3 cells were cultured in 6-well culture plates (1×105 cells/well) overnight before treatment with microneedles for 24 h. The cells were then washed twice with PBS and were collected for direct number assay in a cell counting chamber according to the following formula:
For Live/Dead staining assay, MC3T3-E1 and NIH3T3 cells were cultured in 6-well culture plates (1×105 cells/well) overnight before being treated with microneedles for 24 h. The cells were then washed twice with PBS and stained with the Live/Dead TM Cell Imaging Kit for 30 min. The cells were washed 3 times with PBS and the living cells or dead cells were photographed by fluorescence microscopy (Nikon ECLIPSE Ti-U, Japan).
For Apoptosis assays, MC3T3-E1 and NIH3T3 cells were cultured in 6-well culture plates (1×105 cells/well) overnight before being treated with the microneedles for 24 h. The cells were twice washed with PBS and stained with Annexin V-FITC for 15 min and PI staining solution for 10 min. After stimulation, the apoptotic cells were analyzed by flow cytometry (BD Biosciences).
For ROS staining assay, cellular ROS was detected by DCFH-DA fluorescence probe. MC3T3-E1 and NIH3T3 cells were cultured in 6-well culture plates (1×105 cells/well) overnight before treating with the microneedles for 24 h. The cells were then washed twice with PBS and stained with 10 μM DCFH-DA for 30 min. They were observed and photographed in a fluorescent microscope as above.
There were no statistically significant differences (n=3 biological replicates) in both MC3T3-E1 and NIH3T3 cells after 24 h culturing with or without the MNs patch, indicating that the patch did not significantly affect cell viability (
Furthermore, Live/Dead staining of MC3T3-E1 (
Further analysis of apoptosis assays indicated that there are almost 98% live cells both in MC3T3-E1 (
Analysis of ROS assays has shown no statistically significant differences (n=3 biological replicates), both in MC3T3-E1 (
Together these results indicate that the proposed MNs patch has no significant impact on the MC3T3-E1 and NIH3T3 cells viability, demonstrating their excellent in vitro biocompatibility.
To further evaluate the biocompatibility of the MNs patch, an in vivo study involved an animal model was conducted. For this purpose, hematoxylin and eosin (H&E) staining of the organs, together with CD3 immunofluorescent staining assay of skin tissue were carried out.
For H&E staining, healthy 4-week female BALB/c mice were purchased from an animal laboratory center of Guangdong province and housed in a SPF laboratory animal room. MNs patches were inserted into the back skin of the mice by a thumb press and removed after different insertion times (control, 1 h, 1 day, 3 days, and 7 days). The major organs (including heart, liver, spleen, lung, and kidney) were resected to assess toxicity.
For CD3 Immunofluorescent staining assay of skin tissue, healthy 4-week female BALB/c mice were treated with the microneedles for different times and euthanized so that the skin could be separated and embedded into OCT agent prior to being sectioned at 5 μm for further immunofluorescent staining. Frozen sections were treated with 4% paraformaldehyde and 0.3% Triton X-100 solution. The frozen sections were blocked with 2% BSA solution for 30 min and incubated with anti-CD3 antibody (ab135372) overnight. On the second day, the frozen sections were washed with 1×TBST for 3 times and incubated with goat anti-rabbit IgG Alexa Fluor 568 (A11011, Thermo Fisher) for 60 min. The frozen sections were treated with antifade mounting medium with DAPI (H-1200) and imaged with a confocal microscope (Nikon AIR, Japan).
No apparent pathological changes and toxic effects were seen in the organs within the different insertion times (
CD3 is highly expressed in T cells, which are important in the inflammatory response. CD3 immunofluorescent staining of skin tissue from each group after different insertion times also indicated no significant differences between the MNs patch-inserted groups with different inserting time and the control group (
To evaluate the recovery of the skin following peeling-off, healthy 4-week female BALB/c mice were treated with the microneedles. The micro-holes on the mice were photographed after microneedle insertion and removal. To validate that the MNs do reach as far as the ISF, MNs-treated mice were euthanized and skin samples were collected for H&E assay. H&E images showed that the MNs reached the dermis layer, but not the subcutaneous tissues, demonstrating that MNs had reached the ISF. As is shown in
To evaluate the long-term health monitoring performance of the wearable MN-EGFET patch, Na+ MN-EGFET sensor was used to take on-body measurements for several hours of a healthy individual (
As a reference, sweat was collected after exercise and examined by a commercial device (Na+ meter, HORIBA, B-722) (
The proposed MN-patch can collect health status information of an individual without the need to exercise for sweating, which makes it even more convenient and practical for real-life applications, especially for very sick patients and frail elderly people. With the help of wireless data communication and IoT technology, frail elderly and sick patients' health status can be measured through biosensors and uploaded onto the IoT cloud by computer/smartphone (
To evaluate the applicability of wireless health monitoring with the developed device, the MN-EGFET patch was integrated with the wireless Bluetooth transmitter (ecFlex, Zimmer and Peacock) by using an additionally designed circuit (
To mimic a human body's Na+ concentration fluctuation, we dropped NaCl solutions onto the Na+ sensor in the following order: 10 mM-40 mM-160 mM-10 mM. The concentration change was continually recorded, successfully indicating the sodium change just using a smartphone (
It is appreciated by persons skilled in the art that the present disclosure is not limited by what has been particularly shown and described hereinabove. Rather the scope of the present disclosure includes both combinations and sub-combinations of various features described hereinabove as well as variations and modifications. Therefore, the disclosure is not to be constructed as restricted to the particularly described examples, and the scope and concept of the disclosure will be more readily understood by references to the claims, which follow.
| Number | Date | Country | Kind |
|---|---|---|---|
| 287577 | Oct 2021 | IL | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/IL2022/051120 | 10/25/2022 | WO |
