Claims
- 1. An isolated DNA molecule that hybridizes to the nucleotide sequence of SEQ ID NO: 1 under the following conditions: hybridization in 5.times.SSC and 50% formamide at 42.degree. C., and washing in from about 0.5.times.SSC to about 0.2.times.SSC at 65 .degree. C. wherein said DNA molecule exhibits the regulatory element activity of SEQ ID NO:1.
- 2. The isolated DNA molecule of claim 1 comprising at least 40 contiguous nucleotides of the nucleotide sequence of SEQ ID NO: 1, wherein said isolated DNA molecule exhibits the regulatory element activity.
- 3. The isolated DNA molecule of claim 1 comprising at least 21 contiguous nucleotides of the nucleotide sequence comprising nucleotides 1-481 of SEQ ID NO: 1. wherein said isolated DNA molecule exhibits the regulatory element activity of nucleotides 1-481 of SEQ ID NO:1.
- 4. The isolated DNA molecule of claim 1 comprising at least 19 contiguous nucleotides of the nucleotide sequence comprising nucleotides 1-214 of SEQ ID NO: 1, wherein said isolated DNA molecule exhibits regulatory element activity of nucleotides 1-214 of SEQ ID NO:1.
- 5. The isolated DNA molecule of claim 1 comprising the nucleotide sequence of SEQ ID NO:1.
- 6. An isolated DNA molecule that hybridizes to the nucleotide sequence of SEQ ID NO:2 under the following conditions: hybridization in 5.times.SSC and 50% formamide at 42.degree. C.; and washing in from about 0.5.times.SSC to about 0.2.times.SSC at 65.degree. C., wherein said DNA molecule exhibits the regulatory element activity of SEQ ID NO:2.
- 7. The isolated DNA molecule of claim 6 comprising the nucleotide sequence of SEQ ID NO.2.
- 8. An isolated DNA molecule that hybridizes to the nucleotide sequence of SEQ ID NO:3 under the following conditions: hybridization in 5.times.SSC and 50% formamide at 42.degree. C.; and washing in from about 0.5.times.SSC to about 0.2.times.SSC at 65.degree. C. wherein said DNA molecule exhibits the regulatory element activity of SEQ ID NO:3.
- 9. The isolated DNA molecule of claim 8 comprising the nucleotide sequence of SEQ ID NO:3.
- 10. The isolated DNA molecule of claim 3 comprising nucleotides 1-481 of the nucleotide sequence of SEQ ID NO: 1.
- 11. The isolated DNA molecule of claim 4 comprising nucleotides 1-214 of the nucleotide sequence of SEQ ID NO: 1.
- 12. The isolated DNA molecule of claim 2 comprising nucleotides 215-481 of the nucleotide sequence of SEQ ID NO: 1.
- 13. A vector comprising the isolated DNA molecule of claim 1 and a gene of interest operatively linked thereto, wherein said isolated DNA molecule controls the expression of said gene of interest.
- 14. A transformed plant cell culture comprising the vector of claim 13.
- 15. A transgenic plant transformed with the vector of claim 13.
- 16. A method of expressing a gene of interest within aleurone of a plant comprising:
- i) operatively linking a gene of interest for which expression is desired with a regulatory element obtained from wheat aleurone, to produce a chimeric gene construct; and
- ii) introducing the chimeric gene construct into said plant and allowing for expression of said gene of interest;
- wherein said regulatory element comprises the nucleotide sequence as defined by claim 1.
- 17. A method of expressing a gene of interest within alcurone of a plant comprising:
- i) operatively linking a gene of interest for which expression is desired with a regulatory element obtained from wheat aleurone, to produce a chimeric gene construct; and
- ii) introducing the chimeric gene construct into said plant and allowing for expression of said gene of interest;
- wherein said regulatory element comprises the nucleotide sequence as defined by claim 6.
- 18. A method of expressing a gene of interest within alcurone of a plant comprising:
- i) operatively linking a gene of interest for which expression is desired with a regulatory element obtained from wheat aleurone, to produce a chimeric gene construct; and
- ii) introducing the chimeric gene construct into said plant and allowing for expression of said gene of interest;
- wherein said regulatory element comprises the nucleotide sequence as defined by claim 8.
- 19. A vector comprising the isolated DNA molecule of claim 1.
- 20. An expression vector comprising the isolated DNA molecule of claim 1 operably linked to DNA encoding a protein.
- 21. A vector comprising the isolated DNA molecule of claim 6.
- 22. An expression vector comprising the isolated DNA molecule of claim 6 operably linked to DNA encoding a protein.
- 23. A vector comprising the isolated DNA molecule of claim 8.
- 24. An expression vector comprising the isolated DNA molecule of claim 8 operably linked to DNA encoding a protein.
Priority Claims (1)
Number |
Date |
Country |
Kind |
2230975 |
May 1998 |
CAX |
|
CROSS REFERENCES TO RELATED APPLICATIONS
This application claims priority to a Canadian Patent Application filed on May 7, 1998 Application No. 2,230,975, the application of which is hereby incorporated by reference in its entirety.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5525716 |
Olsen et al. |
Jun 1996 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
2110772 |
Jun 1995 |
CAX |