Claims
- 1. A reference control composition for hematology instruments comprising:
- a) fixed or stabilized red blood cells as a white blood cell surrogate, suspended in an isotonic aqueous suspension medium; and
- b) a lipoprotein in an amount sufficient to provide a composition which gives a white blood cell histogram profile that is substantially that of whole blood.
- 2. The reference control composition of claim 1, further comprising albumin.
- 3. The reference control composition of claim 1, wherein the lipoprotein is a high density lipoprotein.
- 4. The reference control composition of claim 1, wherein the reference control composition is suitable as a reference control for whole blood and further comprises fixed or stabilized red blood cells as the red blood cell component of the whole blood reference control.
- 5. The reference control composition of claim 4, further comprising simulated blood platelets.
- 6. The reference control composition of claim 4, further comprising an antioxidant.
- 7. A method for making a reference control composition for hematology instruments comprising the step of mixing fixed or stabilized red blood cells as a white blood cell surrogate, in an isotonic aqueous suspension medium, and a lipoprotein, wherein the amount of lipoprotein is an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood.
- 8. The method of claim 7, wherein the reference control composition further comprises albumin.
- 9. The method of claim 7, wherein the lipoprotein is a high density lipoprotein.
- 10. The method of claim 7, wherein the reference control composition is suitable as a reference control for whole blood and further comprises fixed or stabilized red blood cells as the red blood cell component of the whole blood reference control.
- 11. The method of claim 10, wherein the reference control composition further comprises simulated blood platelets.
- 12. The method of claim 10, wherein the reference control composition further comprises an antioxidant.
- 13. A reference control composition for hematology instruments comprising:
- a) white blood cells fixed or stabilized and suspended in an isotonic aqueous suspension medium; and
- b) a lipoprotein in an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood.
- 14. The reference control composition of claim 13, further comprising albumin.
- 15. The reference control composition of claim 13, wherein the lipoprotein is a high density lipoprotein.
- 16. The reference control composition of claim 13, wherein the reference control composition is suitable as a reference control for whole blood and further comprises fixed or stabilized red blood cells.
- 17. The reference control composition of claim 16, further comprising simulated blood platelets.
- 18. The reference control composition of claim 16, further comprising an antioxidant.
- 19. A method for making a reference control composition for hematology instruments comprising the step of mixing fixed or stabilized white blood cells, suspended in an isotonic aqueous suspension medium, and a lipoprotein, wherein the amount of lipoprotein is an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood.
- 20. The method of claim 19, wherein the reference control composition further comprises albumin.
- 21. The method of claim 19, wherein the lipoprotein is a high density lipoprotein.
- 22. The method of claim 19, wherein the reference control composition is suitable as a reference control for whole blood and further comprises fixed or stabilized red blood cells.
- 23. The method of claim 22, wherein the reference control composition further comprises simulated blood platelets.
- 24. The method of claim 22, wherein the reference control composition further comprises an antioxidant.
- 25. A method of determining the accuracy of a hematology instrument comprising the steps of:
- a) providing a reference control comprising a blood cell control means suitable as a reference control for blood, wherein the blood cell control means is fixed or stabilized and suspended in an isotonic aqueous suspension medium, and a lipoprotein in an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood;
- b) passing the reference control through the hematology instrument to provide a histogram of the reference control; and
- c) comparing the histogram of step b) with assayed values of the reference control.
- 26. The method of claim 25, wherein the blood cell control means comprises fixed or stabilized white blood cells.
- 27. The method of claim 25, wherein the blood cell control means comprises fixed or stabilized red blood cells as a white blood cell surrogate.
- 28. The method of claim 25, wherein the blood cell control means comprises fixed or stabilized white blood cells and fixed or stabilized red blood cells as a white blood cell surrogate.
- 29. The method of claim 25, wherein the reference control further comprises albumin.
- 30. The method of claim 25, wherein the lipoprotein is a high density lipoprotein.
- 31. The method of claim 25, wherein the reference control is suitable as a reference control for whole blood and further comprises fixed or stabilized red blood cells.
- 32. The method of claims 31, wherein the reference control further comprises simulated blood platelets.
- 33. The method of claim 31, wherein the reference control further comprises an antioxidant.
- 34. A method of determining the accuracy of a hematology instrument comprising the steps of:
- a) providing a reference control comprising a blood cell control means suitable as a reference control for blood, wherein the blood cell control means is fixed or stabilized and suspended in an isotonic aqueous suspension medium, and a composition comprising lipoprotein, wherein the lipoprotein is present in an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood;
- b) passing the reference control through the hematology instrument to provide a histogram of the reference control; and
- c) comparing the histogram of step b) with assayed values of the reference control.
- 35. The method of claim 34, wherein the blood cell control means comprises fixed or stabilized white blood cells.
- 36. The method of claim 34, wherein the blood cell control means comprises fixed or stabilized red blood cells as a white blood cell surrogate.
- 37. The method of claim 34, wherein the blood cell control means comprises fixed or stabilized white blood cells and fixed or stabilized red blood cells as a white blood cell surrogate.
- 38. The method of claim 34, wherein the reference control composition further comprises albumin.
- 39. The method of claim 34, wherein the lipoprotein is a high density lipoprotein.
- 40. The method of claim 34, wherein the blood cell control means is suitable as a reference control for whole blood and further comprises fixed or stabilized red blood cells.
- 41. The method of claim 40, wherein the blood cell control means further comprises simulated blood platelets.
- 42. The method of claim 40, wherein the reference control composition further comprises an antioxidant.
BACKGROUND OF THE INVENTION
This is a continuation of U.S. patent application Ser. No. 08/186,500, filed Jan. 25, 1994, now abandoned, which is a continuation of U.S. patent application Ser. No. 08/052,651, filed Apr. 26, 1993, now abandoned, which is a continuation of U.S. patent application Ser. No. 07/879,429, filed May 7, 1992, now U.S. Pat. No. 5,270,208, which is a continuation-in-part of U.S. patent application Ser. No. 07/697,331, filed May 9, 1991, now abandoned.
US Referenced Citations (28)
Foreign Referenced Citations (2)
Number |
Date |
Country |
WO9317329 |
Sep 1993 |
WOX |
WO9317330 |
Sep 1993 |
WOX |
Non-Patent Literature Citations (4)
Entry |
Greenfield, S.M. et al., "Inhibition of Red Cell Membrane Lipid Peroxidation by Sulphasalazine and 5-Aminosalicylic Acid", Gut 32:1156-1159 (1991). |
Lombarts, A.J.P.F. et al., "A Stable Human Platelet-White Blood Cell Control for the Coulter Model S-Plus II," Clinica. Chimica Acta. 130:95-102 (1982). |
Lombarts, A.J.P.F. et al., "A White Blood Cell Control Long-Term Stability", Clinica. Chimica. Acta. 129:79-83 (1983). |
Negre-Salvayre, A. et al., "Protective Effect of .alpha.-Tocophenrol, Ascorbic Acid and Rutin Against Peroxidative Stress Induced by Oxidized Lipoproteins on Lymphoid Cell Lines," Biochem. Pharmacol. 42:450-453 (1991). |
Continuations (1)
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Number |
Date |
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Parent |
186500 |
Jan 1994 |
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Continuation in Parts (1)
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Number |
Date |
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Parent |
697331 |
May 1991 |
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