Xylose isomerases and their uses

Abstract

This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.

Description

1. BACKGROUND

The efficient, commercial production of biofuels from plant material, such as sugarcane, requires the fermentation of pentoses, such as xylose. Xylose in plant material typically comes from lignocellulose, which is a matrix composed of cellulose, hemicelluloses, and lignin. Lignocellulose is broken down either by acid hydrolysis or enzymatic reaction, yielding xylose in addition to other monosaccharides, such as glucose (Maki et al., 2009, Int. J. Biol. Sci. 5:500-516).

Fungi, especially Saccharomyces cerevisiae, are commercially relevant microorganisms that ferment sugars into biofuels such as ethanol. However, S. cerevisiae does not endogenously metabolize xylose, requiring genetic modifications that allow it to convert xylose into xylulose. Other organisms, whose usefulness in ethanol production is limited, are able to metabolize xylose (Nevigot, 2008, Micobiol. Mol. Biol. Rev. 72:379-412).

Two pathways have been identified for the metabolism of xylose to xylulose in microorganisms: the xylose reductase (XR, EC 1.1.1.307)/xylitol dehydrogenase (XDH, EC 1.1.1.9, 1.1.1.10 and 1.1.1.B19) pathway and the xylose isomerase (XI, EC 5.3.1.5) pathway. Use of the XR/XDH pathway for xylose metabolism creates an imbalance of cofactors (excess NADH and NADP+) limiting the potential output of this pathway for the production of ethanol. The XI pathway, on the otherhand, converts xylose to xylulose in a single step and does not create a cofactor imbalance (Young et al., 2010, Biotechnol. Biofuels 3:24-36).

Because S. cerevisiae does not possess a native XI, it has been desirable to search for an XI in another organism to insert into S. cerevisiae for the purpose of biofuels production. Several XI genes have been discovered, although little or no enzymatic activity upon expression in S. cerevisiae has been a common problem. The XI from Piromyces sp. E2 was the first heterologously expressed XI in S. cerevisiae whose enzymatic activity could be observed (WO 03/062430).

2. SUMMARY

Due to the physiology of S. cerevisiae and the process of commercial biofuel production, there are other characteristics besides activity that are valuable in a commercially useful XI. During fermentation, the pH of the yeast cell and its environment can become more acidic (Rosa and Sa-Correia, 1991, Appl. Environ. Microbiol. 57:830-835). The ability of the XI to function in an acidic environment is therefore highly desirable. Therefore, there is a still a need in the art for XI enzymes with enhanced activity to convert xylose to xylulose for biofuels production under a broader range of commercially relevant conditions.

The present disclosure relates to novel xylose isomerases. The xylose isomerases have desirable characteristics for xylose fermentation, such as high activity, tolerance to acidic conditions (i.e., pH levels below 7, e.g., pH 6.5 or pH 6), or both.

The present disclosure has multiple aspects. In one aspect, the disclosure is directed to XI polypeptides. The polypeptides of the disclosure typically comprise amino acid sequences having at least 70%, 75%, 80%, 85%, 90%, 93%, 95%, 96%, 98%, 99% or 100% sequence identity to any of the XI polypeptides of Table 1, or the catalytic domain or dimerization domain thereof, or are encoded by nucleic acid sequences comprising nucleotide sequences having at least 70%, 75%, 80%, 85%, 90%, 93%, 95%, 96%, 98%, 99% or 100% sequence identity to any of the nucleic acids of Table 1:

TABLE 1
SEQ		Organism	Type of	Catalytic	Dimerization
ID NO:	Clone No.	Classification	Sequence	Domain	Domain
1	1754MI2_001	Bacteroidales	DNA
2	1754MI2_001	Bacteroidales	Amino Acid	2-376	377-437
3	5586MI6_004	Bacteroidales	DNA
4	5586MI6_004	Bacteroidales	Amino Acid	2-376	377-437
5	5749MI1_003	Bacteroidales	DNA
6	5749MI1_003	Bacteroidales	Amino Acid	2-381	382-442
7	5750MI1_003	Bacteroidales	DNA
8	5750MI1_003	Bacteroidales	Amino Acid	2-381	382-442
9	5750MI2_003	Bacteroidales	DNA
10	5750MI2_003	Bacteroidales	Amino Acid	2-381	382-442
11	5586MI5_004	Bacteroides	DNA
12	5586MI5_004	Bacteroides	Amino Acid	2-375	376-435
13	5586MI202_004	Bacteroides	DNA
14	5586MI202_004	Bacteroides	Amino Acid	2-377	378-438
15	5586MI211_003	Bacteroides	DNA
16	5586MI211_003	Bacteroides	Amino Acid	2-376	377-437
17	5606MI1_005	Bacteroides	DNA
18	5606MI1_005	Bacteroides	Amino Acid	2-377	378-438
19	5606MI2_003	Bacteroides	DNA
20	5606MI2_003	Bacteroides	Amino Acid	2-378	379-439
21	5610MI3_003	Bacteroides	DNA
22	5610MI3_003	Bacteroides	Amino Acid	2-377	378-439
23	5749MI2_004	Bacteroides	DNA
24	5749MI2_004	Bacteroides	Amino Acid	2-377	378-438
25	5750MI3_003	Bacteroides	DNA
26	5750MI3_003	Bacteroides	Amino Acid	2-377	378-438
27	5750MI4_003	Bacteroides	DNA
28	5750MI4_003	Bacteroides	Amino Acid	2-377	378-438
29	5751MI4_002	Bacteroides	DNA
30	5751MI4_002	Bacteroides	Amino Acid	2-376	377-437
31	5751MI5_003	Bacteroides	DNA
32	5751MI5_003	Bacteroides	Amino Acid	2-377	378-438
33	5751MI6_004	Bacteroides	DNA
34	5751MI6_004	Bacteroides	Amino Acid	2-377	378-438
35	5586MI22_003	Clostridiales	DNA
36	5586MI22_003	Clostridiales	Amino Acid	2-375	376-439
37	1753MI4_001	Firmicutes	DNA
38	1753MI4_001	Firmicutes	Amino Acid	2-374	375-440
39	1753MI6_001	Firmicutes	DNA
40	1753MI6_001	Firmicutes	Amino Acid	2-374	375-440
41	1753MI35_004	Firmicutes	DNA
42	1753MI35_004	Firmicutes	Amino Acid	2-375	376-441
43	1754MI9_004	Firmicutes	DNA
44	1754MI9_004	Firmicutes	Amino Acid	2-375	376-440
45	1754MI22_004	Firmicutes	DNA
46	1754MI22_004	Firmicutes	Amino Acid	2-375	376-440
47	727MI1_002	Firmicutes	DNA
48	727MI1_002	Firmicutes	Amino Acid	2-372	373-436
49	727MI9_005	Firmicutes	DNA
50	727MI9_005	Firmicutes	Amino Acid	2-374	375-438
51	727MI27_002	Firmicutes	DNA
52	727MI27_002	Firmicutes	Amino Acid	2-374	375-439
53	1753MI2_006	Neocallimastigales	DNA
54	1753MI2_006	Neocallimastigales	Amino Acid	2-376	377-437
55	5586MI3_005	Neocallimastigales	DNA
56	5586MI3_005	Neocallimastigales	Amino Acid	2-376	377-437
57	5586MI91_002	Neocallimastigales	DNA
58	5586MI91_002	Neocallimastigales	Amino Acid	2-376	377-437
59	5586MI194_003	Neocallimastigales	DNA
60	5586MI194_003	Neocallimastigales	Amino Acid	2-376	377-438
61	5586MI198_003	Neocallimastigales	DNA
62	5586MI198_003	Neocallimastigales	Amino Acid	2-375	376-437
63	5586MI201_003	Neocallimastigales	DNA
64	5586MI201_003	Neocallimastigales	Amino Acid	2-376	377-438
65	5586MI204_002	Neocallimastigales	DNA
66	5586MI204_002	Neocallimastigales	Amino Acid	2-375	376-437
67	5586MI207_002	Neocallimastigales	DNA
68	5586MI207_002	Neocallimastigales	Amino Acid	2-375	376-437
69	5586MI209_003	Neocallimastigales	DNA
70	5586MI209_003	Neocallimastigales	Amino Acid	2-375	376-437
71	5586MI214_002	Neocallimastigales	DNA
72	5586MI214_002	Neocallimastigales	Amino Acid	2-375	376-437
73	5751MI3_001	Neocallimastigales	DNA
74	5751MI3_001	Neocallimastigales	Amino Acid	2-375	376-437
75	5753MI3_002	Prevotella	DNA
76	5753MI3_002	Prevotella	Amino Acid	2-376	377-439
77	1754MI1_001	Prevotella	DNA
78	1754MI1_001	Prevotella	Amino Acid	2-377	378-439
79	1754MI3_007	Prevotella	DNA
80	1754MI3_007	Prevotella	Amino Acid	2-377	378-439
81	1754MI5_009	Prevotella	DNA
82	1754MI5_009	Prevotella	Amino Acid	2-375	376-437
83	5586MI1_003	Prevotella	DNA
84	5586MI1_003	Prevotella	Amino Acid	2-377	378-439
85	5586MI2_006	Prevotella	DNA
86	5586MI2_006	Prevotella	Amino Acid	2-377	378-439
87	5586MI8_003	Prevotella	DNA
88	5586MI8_003	Prevotella	Amino Acid	2-377	378-439
89	5586MI14_003	Prevotella	DNA
90	5586MI14_003	Prevotella	Amino Acid	2-377	378-439
91	5586MI26_003	Prevotella	DNA
92	5586MI26_003	Prevotella	Amino Acid	2-377	378-439
93	5586MI86_001	Prevotella	DNA
94	5586MI86_001	Prevotella	Amino Acid	2-376	377-438
95	5586MI108_002	Prevotella	DNA
96	5586MI108_002	Prevotella	Amino Acid	2-377	378-439
97	5586MI182_004	Prevotella	DNA
98	5586MI182_004	Prevotella	Amino Acid	2-377	378-439
99	5586MI193_004	Prevotella	DNA
100	5586MI193_004	Prevotella	Amino Acid	2-376	377-438
101	5586MI195_003	Prevotella	DNA
102	5586MI195_003	Prevotella	Amino Acid	2-376	377-438
103	5586MI216_003	Prevotella	DNA
104	5586MI216_003	Prevotella	Amino Acid	2-376	377-438
105	5586MI197_003	Prevotella	DNA
106	5586MI197_003	Prevotella	Amino Acid	2-376	377-438
107	5586MI199_003	Prevotella	DNA
108	5586MI199_003	Prevotella	Amino Acid	2-376	377-438
109	5586MI200_003	Prevotella	DNA
110	5586MI200_003	Prevotella	Amino Acid	2-376	377-438
111	5586MI203_003	Prevotella	DNA
112	5586MI203_003	Prevotella	Amino Acid	2-376	377-438
113	5586MI205_004	Prevotella	DNA
114	5586MI205_004	Prevotella	Amino Acid	2-376	377-438
115	5586MI206_004	Prevotella	DNA
116	5586MI206_004	Prevotella	Amino Acid	2-376	377-438
117	5586MI208_003	Prevotella	DNA
118	5586MI208_003	Prevotella	Amino Acid	2-376	377-438
119	5586MI210_002	Prevotella	DNA
120	5586MI210_002	Prevotella	Amino Acid	2-374	375-437
121	5586MI212_002	Prevotella	DNA
122	5586MI212_002	Prevotella	Amino Acid	2-376	377-438
123	5586MI213_003	Prevotella	DNA
124	5586MI213_003	Prevotella	Amino Acid	2-376	377-438
125	5586MI215_003	Prevotella	DNA
126	5586MI215_003	Prevotella	Amino Acid	2-376	377-438
127	5607MI1_003	Prevotella	DNA
128	5607MI1_003	Prevotella	Amino Acid	2-376	377-438
129	5607MI2_003	Prevotella	DNA
130	5607MI2_003	Prevotella	Amino Acid	2-376	377-442
131	5607MI3_003	Prevotella	DNA
132	5607MI3_003	Prevotella	Amino Acid	2-376	377-438
133	5607MI4_005	Prevotella	DNA
134	5607MI4_005	Prevotella	Amino Acid	2-376	377-438
135	5607MI5_002	Prevotella	DNA
136	5607MI5_002	Prevotella	Amino Acid	2-376	377-439
137	5607MI6_002	Prevotella	DNA
138	5607MI6_002	Prevotella	Amino Acid	2-376	377-438
139	5607MI7_002	Prevotella	DNA
140	5607MI7_002	Prevotella	Amino Acid	2-376	377-438
141	5608MI1_004	Prevotella	DNA
142	5608MI1_004	Prevotella	Amino Acid	2-376	377-438
143	5608MI2_002	Prevotella	DNA
144	5608MI2_002	Prevotella	Amino Acid	2-375	376-437
145	5608MI3_004	Prevotella	DNA
146	5608MI3_004	Prevotella	Amino Acid	2-376	377-438
147	5609MI1_005	Prevotella	DNA
148	5609MI1_005	Prevotella	Amino Acid	2-376	377-438
149	5610MI1_003	Prevotella	DNA
150	5610MI1_003	Prevotella	Amino Acid	2-376	377-438
151	5610MI2_004	Prevotella	DNA
152	5610MI2_004	Prevotella	Amino Acid	2-376	377-438
153	5751MI1_003	Prevotella	DNA
154	5751MI1_003	Prevotella	Amino Acid	2-376	377-438
155	5751MI2_003	Prevotella	DNA
156	5751MI2_003	Prevotella	Amino Acid	2-376	377-438
157	5752MI1_003	Prevotella	DNA
158	5752MI1_003	Prevotella	Amino Acid	2-376	377-438
159	5752MI2_003	Prevotella	DNA
160	5752MI2_003	Prevotella	Amino Acid	2-376	377-438
161	5752MI3_002	Prevotella	DNA
162	5752MI3_002	Prevotella	Amino Acid	2-376	377-438
163	5752MI5_003	Prevotella	DNA
164	5752MI5_003	Prevotella	Amino Acid	2-376	377-438
165	5752MI6_004	Prevotella	DNA
166	5752MI6_004	Prevotella	Amino Acid	2-376	377-438
167	5753MI1_002	Prevotella	DNA
168	5753MI1_002	Prevotella	Amino Acid	2-376	377-438
169	5753MI2_002	Prevotella	DNA
170	5753MI2_002	Prevotella	Amino Acid	2-376	377-438
171	5753MI4_002	Prevotella	DNA
172	5753MI4_002	Prevotella	Amino Acid	2-376	377-438
173	5752MI4_004	Prevotella	DNA
174	5752MI4_004	Prevotella	Amino Acid	2-376	377-438
175	727MI4_006	Rhizobiales	DNA
176	727MI4_006	Rhizobiales	Amino Acid	2-373	374-435

In specific embodiments, a polypeptide of the disclosure comprises an amino acid sequence having:

• • (1) (a) at least 97% or 98% sequence identity to SEQ ID NO:78 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:78) and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity to SEQ ID NO:78 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:78) and further comprises (i) SEQ ID NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;
  • (2) (a) at least 95%, 97% or 98% sequence identity to SEQ ID NO:96 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:96) and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity to SEQ ID NO:96 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:96) and further comprises (i) SEQ ID NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;
  • (3) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:38 or the catalytic domain thereof (amino acids 2-374 of SEQ ID NO:38), and optionally further comprises one, two, three, four or all five of (i) SEQ ID NO:206 or SEQ ID NO:207; (ii) SEQ ID NO:208; (iii) SEQ ID NO:209; (iv) SEQ ID NO:210; and (iv) SEQ ID NO:211;
  • (4) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:2 or the catalytic domain thereof (amino acids 2-374 of SEQ ID NO:2);
  • (5) at least 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:58 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:58),
  • (6) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:42 or the catalytic domain thereof (amino acids 2-375 of SEQ ID NO:42), and optionally further comprises one, two or all three of (i) SEQ ID NO:206 or SEQ ID NO:207; (ii) SEQ ID NO:210; and (iii) SEQ ID NO:211;
  • (7) (a) at least 97% or 98% sequence identity to SEQ ID NO:84 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:84), and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity to SEQ ID NO:84 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:84) and further comprises (i) SEQ ID NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;
  • (8) (a) at least 97% or 98% sequence identity to SEQ ID NO:80 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:80) and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity to SEQ ID NO:80 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:80) and further comprises (i) SEQ ID NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;
  • (9) at least 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:54 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:54);
  • (10) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:46 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:46), and optionally further comprises SEQ ID NO:206 or SEQ ID NO:207;
  • (11) at least 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:16 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:16);
  • (12) at least 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:82 or the catalytic domain thereof (amino acids 2-375 of SEQ ID NO:82); and/or
  • (13) at least 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:32 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:32).

The XIs of the disclosure can be characterized in terms of their activity. In some embodiments, a XI of the disclosure has at least 1.3 times the activity of the Orpinomyces sp. XI assigned Genbank Accession No. 169733248 (“Op-XI”) at pH 7.5, for example using the assay described in any of Examples 4, 6 and 7. In certain specific embodiments, a XI of the disclosure has an activity ranging from 1.25 to 3.0 times, from 1.5 to 3 times, from 1.5 to 2.25 times, or from 1.75 to 3 times the activity of Op-XI at pH 7.5.

The XIs of the disclosure can also be characterized in terms of their tolerance to acidic environments (e.g., at a pH of 6.5 or 6). In some embodiments, a XI of the disclosure has at least 1.9 times the activity of the Op-XI at pH 6, for example using the assay described in Example 7. In certain specific embodiments, a XI of the disclosure has an activity ranging from 1.9 to 4.1 times, from 2.4 to 4.1 times, from 2.4 to 3.9 times, or 2.4 to 4.1 times the activity of Op-XI at pH 6.

Tolerance to acidic environments can also be characterized as a ratio of activity at pH 6 to activity at pH 7.5 (“a pH 6 to pH 7.5 activity ratio”), for example as measured using the assay of Example 7. In some embodiments, the pH 6 to pH 7.5 activity ratio is at least 0.5 or at least 0.6. In various embodiments, the pH 6 to pH 7.5 activity ratio is 0.5-0.9 or 0.6-0.9.

In another aspect, the disclosure is directed to a nucleic acid which encodes a XI polypeptide of the disclosure. In various embodiments, the nucleic acid comprises a nucleotide sequence with at least 70%, 75%, 80%, 85%, 90%, 93%, 95%, 96%, 98%, 99% or 100% sequence identity to the nucleotide sequence of any one of SEQ ID NOS:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, and 175, or the portion of any of the foregoing sequences encoding a XI catalytic domain or dimerization domain.

In other aspects, the disclosure is directed to a vector comprising a XI-encoding nucleotide sequence, for example a vector having an origin of replication and/or a promoter sequence operably linked to the XI-encoding nucleotide sequence. The promoter sequence can be one that is operable in a eukaryotic cell, for example in a fungal cell. In some embodiments, the promoter is operable in yeast (e.g., S. cerevisiae) or filamentous fungi.

In yet another aspect, the disclosure is directed to a recombinant cell comprising a nucleic acid that encodes a XI polypeptide. Particularly, the cell is engineered to express any of the XI polypeptides described herein. The recombinant cell may be of any species, and is preferably a eukaryotic cell, for example a yeast cell. Suitable genera of yeast include Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Klockera, Schwanniomyces, Issatchenkia and Yarrowia. In specific embodiments, the recombinant cell is a S. cerevisiae, S. bulderi, S. barnetti, S. exiguus, S. uvarum, S. diastaticus, K. lactis, I. orientalis, K. marxianus or K. fragilis. Suitable genera of filamentous fungi include Aspergillus, Penicillium, Rhizopus, Chrysosporium, Myceliophthora, Trichoderma, Humicola, Acremonium and Fusarium. In specific embodiments, the recombinant cell is an Aspergillus niger, Aspergillus oryzae, Trichoderma reesei, Penicillium chrysogenum, Myceliophthora thermophila, or Rhizopus oryzae.

The recombinant cell may also be mutagenized or engineered to include modifications other than the recombinant expression of XI, particularly those that make the cell more suited to utilize xylose in a fermentation pathway. Exemplary additional modifications create one, two, three, four, five or even more of the following phenotypes: (a) increase in xylose transport into the cell; (b) increase in aerobic growth rate on xylose; (c) increase in xylulose kinase activity; (d) increase in flux through the pentose phosphate pathway into glycolysis, (e) decrease in aldose reductase activity, (f) decrease in sensitivity to catabolite repression, (g) increase in tolerance to biofuels, e.g., ethanol, (h) increase tolerance to intermediate production (e.g., xylitol), (i) increase in temperature tolerance, (j) osmolarity of organic acids, and (k) a reduced production of byproducts.

Increases in activity can be achieved by increased expression levels, for example expression of a hexose or pentose (e.g., xylose) transporter, a xylulose kinase, a glycolytic enzyme, or an ethanologenic enzyme is increased. The increased expression levels are achieved by overexpressing an endogenous protein or by expressing a heterologous protein.

Other modifications to the recombinant cell that are part of the disclosure are modifications that decrease the activity of genes or pathways in the recombinant cell. Preferably, the expression levels of one, two, three or more of the genes for hexose kinase, MIG-1, MIG-2, XR, aldose reductase, and XDH are reduced. Reducing gene activity can be achieved by a targeted deletion or disruption of the gene (and optionally reintroducing the gene under the control of a different promoter that drives lower levels of expression or inducible expression).

In yet other aspects, the disclosure is directed to methods of producing fermentation products, for example one or more of ethanol, butanol, diesel, lactic acid, 3-hydroxy-propionic acid, acrylic acid, acetic acid, succinic acid, citric acid, malic acid, fumaric acid, itaconic acid, an amino acid, 1,3-propane-diol, ethylene, glycerol, a β-lactam antibiotic and a cephalosporin. Typically, a cell that recombinantly expresses a XI of the disclosure is cultured in a xylose-containing medium, for example a medium supplemented with a lignocellulosic hydrolysate. The media may also contain glucose, arabinose, or other sugars, particularly those derived from lignocellulose. The media may be of any pH, particularly a pH between 3.0 and 9.0, preferably between 4.0 and 8.0, more preferably between 5.0 and 8.0, even more preferably between 6.0 and 7.5. The culture may occur in any media where the culture is under anaerobic or aerobic conditions, preferably under anaerobic conditions for production of compounds mentioned above and aerobically for biomass/cellular production. Optionally, the methods further comprise recovering the fermentation product produced by the recombinant cell.

3. BRIEF DESCRIPTION OF THE FIGURES

FIGS. 1A-1B are maps for the vector pMEV-ΔxylA (MEV3 xylA del) and PCR-BluntII-TOPO-xylA, respectively, used in the activity-based screen for XIs.

FIG. 2 illustrates the experimental strategy for the two-step marker exchange approach.

FIG. 3 is a map of the vector p426PGK1 for expressing XI in yeast strain, Saccharomyces cerevisiae CEN.PK2-1Ca (ATCC: MYA1108).

FIG. 4 shows the growth rates on xylose containing media of selected clones expressed in yeast strain, Saccharomyces cerevisiae CEN.PK2-1Ca (ATCC: MYA1108).

FIGS. 5A-5D are maps for the vectors pYDAB-006, pYDURA01, pYDPt-005 and pYDAB-0006, respectively, all used in creating strains of industrial S. cerevisiae strain yBPA130 with a single genomic copy of select XI clones.

FIG. 6 is a map of vector YDAB008-rDNA for multiple XI integration into S. cerevisiae strain yBPB007 and yBPB008.

FIGS. 7A-7B show monosaccharide (including xylose) utilization and ethanol production by strains of industrial S. cerevisiae with multiple copies of XI clones integrated into ribosomal DNA loci.

FIG. 8: Production of ethanol from glycolytic and pentose phosphate (“PPP”) pathways. Not all steps are shown. For example, glyceraldehyde-3-phosphate is converted to pyruvate via a series of glycolytic steps: (1) glyceraldehyde-3-phosphate to 3-phospho-D-glycerol-phosphate catalyzed by glyceraldehyde-3-phosphate dehydrogenase (TDH1-3); (2) 3-phospho-D-glycerol-phosphate to 3-phosphoglycerate catalyzed by 3-phosphoglycerate kinase (PGK1); (3) 3-phosphoglycerate to 2-phosphoglycerate catalyzed by phosphoglycerate mutase (GPM1); (4) 2-phosphoglycerate to phosphoenolpyruvate catalyzed by enolase (ENO1; ENO2); and (5) phosphoenolpyruvate to pyruvate calatyzed by pyruvate kinase (PYK2; CDC19). Other abbreviations: DHAP=dihydroxy-acetone-phosphate; GPD=Glycerol-3-phosphate dehydrogenase; RHR2/HOR2=DL-glycerol-3-phosphatase; XI=xylose isomerase; GRE=xylose reductase/aldose reductase; XYL=xylitol dehydrogenase; XKS=xylulokinase; PDC=pyruvate decarboxylase; ADH=alcohol dehydrogenase; ALD=aldehyde dehydrogenase; FIXK=hexokinase; PGI=phosphoglucose isomerase; PFK=phosphofructokinase; FBA=aldolase; TPI=triosephosphate isomerase; ZWF=glucose-6 phosphate dehydrogenase; SOL=6-phosphogluconolactonase; GND=6-phosphogluconate dehydrogenase; RPE=D-ribulose-5-Phosphate 3-epimerase; RKI=ribose-5-phosphate ketol-isomerase; TKL=transketolase; TAL=transaldolase. Heavy dashed arrows indicate reactions and corresponding enzymes that can be reduced or eliminated to increase xylose utilization, particularly in the production of ethanol, and heavy solid arrows indicate reactions and corresponding enzymes that can be increased to increase xylose utilization, particularly in the production of ethanol. The enzymes shown in FIG. 8 are encoded by S. cerevisiae genes. The S. cerevisiae genes are used for exemplification purposes. Analogous enzymes and modifications in other organisms are within the scope of the present disclosure.

4. DETAILED DESCRIPTION

4.1 Xylose Isomerase Polypeptides

A “xylose isomerase” or “XI” is an enzyme that catalyzes the direct isomerisation of D-xylose into D-xylulose and/or vice versa. This class of enzymes is also known as D-xylose ketoisomerases. A xylose isomerase herein may also be capable of catalyzing the conversion between D-glucose and D-fructose (and accordingly may therefore be referred to as a glucose isomerase).

A “XI polypeptide of the disclosure” or a “XI of the disclosure” is a xylose isomerase having an amino acid sequence that is related to any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, or 176. In some embodiments, the xylose isomerase of the disclosure has an amino acid sequence that is at least about 70%, at least 80%, at least 90%, at least 95%, at least 96%, at least 98%, or at least 99% sequence identity thereto, or to a catalytic or dimerization domain thereof. The xylose isomerase of the disclosure can also have 100% sequence identity to one of the foregoing sequences.

The disclosure provides isolated, synthetic or recombinant XI polypeptides comprising an amino acid sequence having at least about 80%, e.g., at least about 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or complete (100%) sequence identity to a polypeptide of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, or 176, over a region of at least about 10, e.g., at least about 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, or 350 residues, or over the full length of the polypeptide, over the length of catalytic domain, or over the length of the dimerization domain.

The XI polypeptides of the disclosure can be encoded by a nucleic acid sequence having at least about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, or about 90% sequence identity to 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, or 175, or by a nucleic acid sequence capable of hybridizing under high stringency conditions to a complement of SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, or 175, or to a fragment thereof. Exemplary nucleic acids of the disclosure are described in Section 4.2 below.

In specific embodiments, a polypeptide of the disclosure comprises an amino acid sequence having:

• • (1) (a) at least 97% or 98% sequence identity to SEQ ID NO:78 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:78) and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity to SEQ ID NO:78 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:78) and further comprises (i) SEQ ID NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;
  • (2) (a) at least 95%, 97% or 98% sequence identity to SEQ ID NO:96 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:96) and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity to SEQ ID NO:96 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:96) and further comprises (i) SEQ ID NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;
  • (3) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:38 or the catalytic domain thereof (amino acids 2-374 of SEQ ID NO:38), and optionally further comprises one, two, three, four or all five of (i) SEQ ID NO:206 or SEQ ID NO:207; (ii) SEQ ID NO:208; (iii) SEQ ID NO:209; (iv) SEQ ID NO:210; and (iv) SEQ ID NO:211;
  • (4) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:2 or the catalytic domain thereof (amino acids 2-374 of SEQ ID NO:2);
  • (5) at least 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:58 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:58),
  • (6) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:42 or the catalytic domain thereof (amino acids 2-375 of SEQ ID NO:42), and optionally further comprises one, two or all three of (i) SEQ ID NO:206 or SEQ ID NO:207; (ii) SEQ ID NO:210; and (iii) SEQ ID NO:211;
  • (7) (a) at least 97% or 98% sequence identity to SEQ ID NO:84 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:84), and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity to SEQ ID NO:84 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:84) and further comprises (i) SEQ ID NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;
  • (8) (a) at least 97% or 98% sequence identity to SEQ ID NO:80 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:80) and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity to SEQ ID NO:80 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:80) and further comprises (i) SEQ ID NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;
  • (9) at least 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:54 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:54);
  • (10) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:46 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:46), and optionally further comprises SEQ ID NO:206 or SEQ ID NO:207;
  • (11) at least 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:16 or the catalytic domain thereof (amino acids 2-376 of SEQ ID NO:16);
  • (12) at least 85%, 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:82 or the catalytic domain thereof (amino acids 2-375 of SEQ ID NO:82); and/or
  • (13) at least 90%, 93%, 95%, 97% or 98% sequence identity to SEQ ID NO:32 or the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:32).

An example of an algorithm that is suitable for determining sequence similarity is the BLAST algorithm, which is described in Altschul et al., 1990, J. Mol. Biol. 215:403-410. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence that either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. These initial neighborhood word hits act as starting points to find longer HSPs containing them. The word hits are expanded in both directions along each of the two sequences being compared for as far as the cumulative alignment score can be increased. Extension of the word hits is stopped when: the cumulative alignment score falls off by the quantity X from a maximum achieved value; the cumulative score goes to zero or below; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLAST program uses as defaults a word length (W) of 11, the BLOSUM62 scoring matrix (see Henikoff & Henikoff, 1992, Proc. Nat'l. Acad. Sci. USA 89:10915-10919) alignments (B) of 50, expectation (E) of 10, M′5, N′-4, and a comparison of both strands.

Any of the amino acid sequences described herein can be produced together or in conjunction with at least 1, e.g., at least (or up to) 2, 3, 5, 10, or 20 heterologous amino acids flanking each of the C- and/or N-terminal ends of the specified amino acid sequence, and or deletions of at least 1, e.g., at least (or up to) 2, 3, 5, 10, or 20 amino acids from the C- and/or N-terminal ends of a XI of the disclosure.

The XIs of the disclosure can be characterized in terms of their activity. In some embodiments, a XI of the disclosure has at least 1.3 times the activity of the Orpinomyces sp. XI assigned Genbank Accession No. 169733248 (“Op-XI”) at pH 7.5, for example using the assay described in any of Examples 4, 6 and 7. In certain specific embodiments, a XI of the disclosure has an activity ranging from 1.25 to 3.0 times, from 1.5 to 3 times, from 1.5 to 2.25 times, or from 1.75 to 3 times the activity of Op-XI at pH 7.5.

The XIs of the disclosure can also be characterized in terms of their tolerance to acidic environments (e.g., at a pH of 6.5 or 6). In some embodiments, a XI of the disclosure has at least 1.9 times the activity of the Op-XI at pH 6, for example using the assay described in Example 7. In certain specific embodiments, a XI of the disclosure has an activity ranging from 1.9 to 4.1 times, from 2.4 to 4.1 times, from 2.4 to 3.9 times, or 2.4 to 4.1 times the activity of Op-XI at pH6.

Tolerance to acidic environments can also be characterized as a ratio of activity at pH 6 to activity at pH 7.5 (“a pH 6 to pH 7.5 activity ratio”), for example as measured using the assay of Example 7. In some embodiments, the pH 6 to pH 7.5 activity ratio is at least 0.5 or at least 0.6. In various embodiments, the pH 6 to pH 7.5 activity ratio is 0.5-0.9 or 0.6-0.9.

The xylose isomerases of the disclosure can have one or more (e.g., up to 2, 3, 5, 10, or 20) conservative amino acid substitutions relative to the polypeptide of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, or 176 or to the portion thereof of discussed above. The conservative substitutions can be chosen from among a group having a similar side chain to the reference amino acid. For example, a group of amino acids having aliphatic side chains is glycine, alanine, valine, leucine, and isoleucine; a group of amino acids having aliphatic-hydroxyl side chains is serine and threonine; a group of amino acids having amide-containing side chains is asparagine and glutamine; a group of amino acids having aromatic side chains is phenylalanine, tyrosine, and tryptophan; a group of amino acids having basic side chains is lysine, arginine, and histidine; and a group of amino acids having sulphur-containing side chains is cysteine and methionine. Accordingly, exemplary conservative substitutions for each of the naturally occurring amino acids are as follows: ala to ser; arg to lys; asn to gln or his; asp to glu; cys to ser or ala; gln to asn; glu to asp; gly to pro; his to asn or gln; ile to leu or val; leu to ile or val; lys to arg; gln or glu; met to leu or ile; phe to met, leu or tyr; ser to thr; thr to ser; trp to tyr; tyr to trp or phe; and, val to ile or leu.

The present disclosure also provides a fusion protein that includes at least a portion (e.g., a fragment or domain) of a XI polypeptide of the disclosure attached to one or more fusion segments, which are typically heterologous to the XI polypeptide. Suitable fusion segments include, without limitation, segments that can provide other desirable biological activity or facilitate purification of the XI polypeptide (e.g., by affinity chromatography). Fusion segments can be joined to the amino or carboxy terminus of a XI polypeptide. The fusion segments can be susceptible to cleavage.

4.2 Xylose Isomerase Nucleic Acids

A “XI nucleic acid of the disclosure” is a nucleic acid encoding a xylose isomerase of the disclosure. In certain embodiments, the xylose isomerase nucleic acid of the disclosure is encoded by a nucleotide sequence of any one of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, or 175, or a sequence having at least about 70%, at least 80%, at least 90%, at least 95%, at least 96%, at least 98%, or at least 99% sequence identity thereto. The xylose isomerase nucleic acid of the disclosure can also have 100% sequence identity to one of the foregoing sequences.

The present disclosure provides nucleic acids encoding a polypeptide of the disclosure, for example one described in Section 4.1 above. The disclosure provides isolated, synthetic or recombinant nucleic acids comprising a nucleic acid sequence having at least about 70%, e.g., at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%; 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or complete (100%) sequence identity to a nucleic acid of SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, or 175, over a region of at least about 10, e.g., at least about 15, 20, 25, 30, 35, 40, 45, 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150, 1200, 1250, 1300, 1350, 1400, 1450, 1500, 1550, 1600, 1650, 1700, 1750, 1800, 1850, 1900, 1950, or 2000 nucleotides.

Nucleic acids of the disclosure also include isolated, synthetic or recombinant nucleic acids encoding a XI polypeptide having the sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, or 176, and subsequences thereof (e.g., a conserved domain or a catalytic domain), and variants thereof.

To increase the likelihood that a XI polypeptide is recombinantly expressed, a XI nucleic acid may be adapted to optimize its codon usage to that of the chosen cell. Several methods for codon optimization are known in the art. For expression in yeast, an exemplary method to optimize codon usage of the nucleotide sequences to that of the yeast is a codon pair optimization technology as disclosed in WO2006/077258 and/or WO2008/000632. WO2008/000632 addresses codon-pair optimization. Codon-pair optimization is a method wherein the nucleotide sequences encoding a polypeptide are modified with respect to their codon-usage, in particular the codon-pairs that are used, to obtain improved expression of the nucleotide sequence encoding the polypeptide and/or improved production of the encoded polypeptide. Codon pairs are defined as a set of two subsequent triplets (codons) in a coding sequence.

4.3 Host Cells and Recombinant Expression

The disclosure also provides host cells transformed with a XI nucleic acid and recombinant host cells engineered to express XI polypeptides. The XI nucleic acid construct may be extrachromosomal, on a plasmid, which can be a low copy plasmid or a high copy plasmid. The nucleic acid construct may be maintained episomally and thus comprise a sequence for autonomous replication, such as an autosomal replication sequence. Alternatively, a XI nucleic acid may be integrated in one or more copies into the genome of the cell. Integration into the cell's genome may occur at random by non-homologous recombination but preferably, the nucleic acid construct may be integrated into the cell's genome by homologous recombination as is well known in the art. In certain embodiments, the host cell is bacterial or fungal (e.g., a yeast or a filamentous fungus).

Suitable host cells of the bacterial genera include, but are not limited to, cells of Escherichia, Bacillus, Lactobacillus, Pseudomonas, and Streptomyces. Suitable cells of bacterial species include, but are not limited to, cells of Escherichia coli, Bacillus subtilis, Bacillus licheniformis, Lactobacillus brevis, Pseudomonas aeruginosa, and Streptomyces lividans.

Suitable host cells of the genera of yeast include, but are not limited to, cells of Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Klockera, Schwanniomyces, Phaffia, Issatchenkia and Yarrowia. In specific embodiments, the recombinant cell is a S. cerevisiae, C. albicans, S. pombe, S. bulderi, S. barnetti, S. exiguus, S. uvarum, S. diastaticus, H. polymorpha, K. lactis, I. orientalis, K. marxianus, K. fragilis, P. pastoris, P. canadensis, K. marxianus or P. rhodozyma. Exemplary yeast strains that are suitable for recombinant XI expression include, but are not limited to, Lallemand LYCC 6391, Lallemand LYCC 6939, Lallemand LYCC 6469, (all from Lallemand, Inc., Montreal, Canada); NRRL YB-1952 (ARS (NRRL) Collection, U.S. Department of Agriculture); and BY4741.

Suitable host cells of filamentous fungi include all filamentous forms of the subdivision Eumycotina. Suitable cells of filamentous fungal genera include, but are not limited to, cells of Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysoporium, Coprinus, Coriolus, Corynascus, Chaetomium, Cryptococcus, Filobasidium, Fusarium, Gibberella, Humicola, Hypocrea, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Scytaldium, Schizophyllum, Sporotrichum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes, and Trichoderma. In certain aspects, the recombinant cell is a Trichoderma sp. (e.g., Trichoderma reesei), Penicillium sp., Humicola sp. (e.g., Humicola insolens); Aspergillus sp. (e.g., Aspergillus niger), Chrysosporium sp., Fusarium sp., or Hypocrea sp. Suitable cells can also include cells of various anamorph and teleomorph forms of these filamentous fungal genera.

Suitable cells of filamentous fungal species include, but are not limited to, cells of Aspergillus awamori, Aspergillus fumigatus, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Chrysosporium lucknowense, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium sulphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Coprinus cinereus, Coriolus hirsutus, Humicola insolens, Humicola lanuginosa, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Neurospora intermedia, Penicillium purpurogenum, Penicillium canescens, Penicillium solitum, Penicillium funiculosum, Phanerochaete chrysosporium, Phlebia radiate, Pleurotus eryngii, Talaromyces flavus, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei, and Trichoderma viride.

Typically, for recombinant expression, the XI nucleic acid will be operably linked to one or more nucleic acid sequences capable of providing for or aiding the transcription and/or translation of the XI sequence, for example a promoter operable in the organism in which the XI is to be expressed. The promoters can be homologous or heterologous, and constitutive or inducible.

Preferably, the XI polypeptide is expressed in the cytosol and therefore lacks a mitochondrial or peroxisomal targeting signal.

Where recombinant expression in a filamentous fungal host is desired, the promoter can be a fungal promoter (including but not limited to a filamentous fungal promoter), a promoter operable in plant cells, a promoter operable in mammalian cells.

As described in U.S. provisional application No. 61/553,901, filed Oct. 31, 2011, the contents of which are hereby incorporated in their entireties, promoters that are constitutively active in mammalian cells (which can derived from a mammalian genome or the genome of a mammalian virus) are capable of eliciting high expression levels in filamentous fungi such as Trichoderma reesei. An exemplary promoter is the cytomegalovirus (“CMV”) promoter.

As described in U.S. provisional application No. 61/553,897, filed Oct. 31, 2011, the contents of which are hereby incorporated in their entireties, promoters that are constitutively active in plant cells (which can derived from a plant genome or the genome of a plant virus) are capable of eliciting high expression levels in filamentous fungi such as Trichoderma reesei. Exemplary promoters are the cauliflower mosaic virus (“CaMV”) 35S promoter or the Commelina yellow mottle virus (“CoYMV”) promoter.

Mammalian, mammalian viral, plant and plant viral promoters can drive particularly high expression when the associated 5′ UTR sequence (i.e., the sequence which begins at the transcription start site and ends one nucleotide (nt) before the start codon), normally associated with the mammalian or mammalian viral promoter is replaced by a fungal 5′ UTR sequence.

The source of the 5′ UTR can vary provided it is operable in the filamentous fungal cell. In various embodiments, the 5′ UTR can be derived from a yeast gene or a filamentous fungal gene. The 5′ UTR can be from the same species, one other component in the expression cassette (e.g., the promoter or the XI coding sequence), or from a different species. The 5′ UTR can be from the same species as the filamentous fungal cell that the expression construct is intended to operate in. In an exemplary embodiment, the 5′ UTR comprises a sequence corresponding to a fragment of a 5′ UTR from a T. reesei glyceraldehyde-3-phosphate dehydrogenase (gpd). In a specific embodiment, the 5′ UTR is not naturally associated with the CMV promoter

Examples of other promoters that can be used include, but are not limited to, a cellulase promoter, a xylanase promoter, the 1818 promoter (previously identified as a highly expressed protein by EST mapping Trichoderma). For example, the promoter can suitably be a cellobiohydrolase, endoglucanase, or β-glucosidase promoter. A particularly suitable promoter can be, for example, a T. reesei cellobiohydrolase, endoglucanase, or β-glucosidase promoter. Non-limiting examples of promoters include a cbh1, cbh2, egl1, egl2, egl3, egl4, egl5, pkil, gpdl, xyn1, or xyn2 promoter.

For recombinant expression in yeast, suitable promoters for S. cerevisiae include the MFa1 promoter, galactose inducible promoters such as the GAL1, GAL7 and GAL10 promoters, glycolytic enzyme promoters including the TPI and PGK promoters, the TDH3 promoter, the TEF1 promoter, the TRP1 promoter, the CYCI promoter, the CUP1 promoter, the PHOS promoter, the ADH1 promoter, and the HSP promoter. Promoters that are active at different stage of growth or production (e.g., idiophase or trophophase) can also be used (see, e.g., Puig et al., 1996, Biotechnology Letters 18(8):887-892; Puig and Perez-Ortin, 2000, Systematic and Applied Microbiology 23(2):300-303; Simon et al., 2001, Cell 106:697-708; Wittenberg and Reed, 2005, Oncogene 24:2746-2755). A suitable promoter in the genus Pichia sp. is the AOXI (methanol utilization) promoter.

The engineered host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants, or amplifying the nucleic acid sequence encoding the XI polypeptide. Culture conditions, such as temperature, pH and the like, are those previously used with the host cell selected for expression, and will be apparent to those skilled in the art. As noted, many references are available for the culture and production of many cells, including cells of bacterial and fungal origin. Cell culture media in general are set forth in Atlas and Parks (eds.), 1993, The Handbook of Microbiological Media, CRC Press, Boca Raton, Fla., which is incorporated herein by reference. For recombinant expression in filamentous fungal cells, the cells are cultured in a standard medium containing physiological salts and nutrients, such as described in Pourquie et al., 1988, Biochemistry and Genetics of Cellulose Degradation, eds. Aubert, et al., Academic Press, pp. 71-86; and Ilmen et al., 1997, Appl. Environ. Microbiol. 63:1298-1306. Culture conditions are also standard, e.g., cultures are incubated at 30° C. in shaker cultures or fermenters until desired levels of XI expression are achieved. Preferred culture conditions for a given filamentous fungus may be found in the scientific literature and/or from the source of the fungi such as the American Type Culture Collection (ATCC). After fungal growth has been established, the cells are exposed to conditions effective to cause or permit the expression of a XI.

In cases where a XI coding sequence is under the control of an inducible promoter, the inducing agent, e.g., a sugar, metal salt or antibiotics, is added to the medium at a concentration effective to induce XI expression.

In addition to recombinant expression of a XI polypeptide, a host cell of the disclosure may further include one or more genetic modifications that increase the cell's ability to utilize xylose as a substrate in a fermentation process. Exemplary additional modifications create one, two, three, four, five or even more of the following phenotypes: (a) increase in xylose transport into the cell; (b) increase in aerobic growth rate on xylose; (c) increase in xylulose kinase activity; (d) increase in flux through the pentose phosphate pathway into glycolysis, (e) modulating in aldose reductase activity, (f) decrease in sensitivity to catabolite repression, (g) increase in tolerance to biofuels, e.g., ethanol, (h) increase tolerance to intermediate production (for example xylitol), (i) increase in temperature tolerance, (j) osmolarity of organic acids, and (k) a reduced production of byproducts.

As illustrated below, a modification that results in one or more of the foregoing phenotypes can be a result of increasing or decreasing expression of an endogenous protein (e.g., by at least a factor of about 1.1, about 1.2, about 1.5, about 2, about 5, about 10 or about 20) or a result of introducing expression of a heterologous polypeptide. For avoidance of doubt, “decreasing” or “reducing” gene expression encompasses eliminating expression. Decreasing (or reducing) the expression of an endogenous protein can be accomplished by inactivating one or more (or all) endogenous copies of a gene in a cell. A gene can be inactivated by deletion of at least part of the gene or by disruption of the gene. This can be achieved by deleting the some or all of a gene coding sequence or regulatory sequence whose deletion results in a reduction of gene expression in the cell. Examples of modifications that increase xylose utilization or yield of fermentation product are described below.

Increasing Xylose Transport:

Xylose transport can be increased directly or indirectly. For example, a recombinant cell may include one or more genetic modifications that result in expression of a xylose transporter. Exemplary transporters include, but are not limited to GXF1, SUT1 and At6g59250 from Candida intermedia, Pichia stipitis (now renamed Scheffersomyces stipitis; the terms are used interchangeably herein) and Arabidopsis thaliana, respectively (Runquist et al., 2010, Biotechnol. Biofuels 3:5), as well as HXT4, HXT5, HXT7, GAL2, AGT1, and GXF2 (see, e.g., Matsushika et al., 2009, Appl. Microbiol. Biotechnol. 84:37-53). Other transporters include PsAraT, SUT2-4 and XUT1-5 from P. stiptis; GXS1 from Candida intermedia; Xy1HP and DEHAOD02167 from Debaryomyces hansenii; and YALI0C06424 from Yarrowia lipolytica (see, e.g., Young et al., 2011, Appl. Environ. Microbiol. 77:3311-3319). Xylose transport can also be increased by (over-) expression of low-affinity hexose transporters, which are capable of non-selectively transporting sugars, including xylose, into the cell once glucose levels are low (e.g., 0.2-1.0 g/l); and includes CgHXT1-CgHXTS from Colletotrichum graminicola. The foregoing modifications can be made singly or in combinations of two, three or more modifications.

Increasing Xylulose Kinase Activity:

Xylulose kinase activity can be increased by overexpression of a xylulose kinase, e.g., xylulose kinase (XKS1; Saccharomyces genome database (“SGD”) accession no. YGR194C) of S. cerevisiae, particularly where the recombinant cell is a yeast cell. In one embodiment, a S. cerevisiae cell is engineered to include at least 2 additional copies of xylulose kinase under the control of a strong constitutive promoter such as TDH3, TEF1 or PGK1. In another embodiment, overexpression of an endogenous xylulose kinase was engineered. This xylulose kinase having improved kinetic activities through the use of protein engineering techniques known by those skilled in the art.

Increasing Flux Through the Pentose Phosphate Pathway:

This can be achieved by increasing expression of one or more genes in the pentose phosphate pathway, for example S. cerevisiae transaldolase TAL1 (SGD accession no. YLR354C), transketolase TKL1 (SGD accession no. YPR074C), ribulose 5-phosphate epimerase RPE1 (SGD accession no. YJL121C) and ribose-5-phosphate ketoisomerase RKI1 (SGD accession no. YOR095C) and/or one or more genes to increase glycolytic flux, for example S. cerevisiae pyruvate kinase PYK1/CDC19 (SGD accession no. YAL038W), pyruvate decarboxylase PDC1 (SGD accession no. YLR044C), pyruvate decarboxylase PDC5 (SGD accession no. YLR134W), pyruvate decarboxylase PDC6 (SGD accession no. YGR087C), the alcohol dehydrogenases ADH1-5 (SGD accession nos. YOL086C, YMR303C, YMR083W, YGL256W, and YBR145W, respectively), and hexose kinase HXK1-2 (SGD accession nos. YFR053C and YGL253W, respectively). In one embodiment, the yeast cell has one additional copy each of TAL1, TKL1, RPE1 and RKI1 from S. cerevisiae under the control of strong constitutive promoters (e.g., PGK1, TDH3, TEF1); and may also include improvements to glycolytic flux (e.g., increased copies of genes such as PYK1, PDC1, PDC5, PDC6, ADH1-5) and glucose-6-phosphate and hexokinase. The foregoing modifications can be made singly or in combinations of two, three or more modifications.

Modulating Aldose Reductase Activity:

A recombinant cell can include one or more genetic modifications that increase or reduce (unspecific) aldose reductase (sometimes called aldo-keto reductase) activity. Aldose reductase activity can be reduced by one or more genetic modifications that reduce the expression of or inactivate a gene encoding an aldose reductase, for example S. cerevisiae GRE3 (SGD accession no. YHR104W).

In certain embodiments, GRE3 expression is reduced. In one aspect, the recombinant cell is a yeast cell in which the GRE3 gene is deleted. Deletion of GRE3 decreased xylitol yield by 49% and biomass production by 31%, but increased ethanol yield by 19% (Traff-Bjerre et al., 2004, Yeast 21:141-150). In another aspect, the recombinant cell is a yeast cell which has a reduction in expression of GRE3. Reducing GRE3 expression has been shown to result in a two-fold decrease in by-product (i.e., xylitol) formation and an associated improvement in ethanol yield (Traff et al., 2001, Appl. Environ. Microbiol. 67:5668-5674).

In another embodiment, the recombinant cell is a cell (optionally but not necessarily a yeast cell) in which GRE3 is overexpressed. In a study analyzing the effect of GRE3 overexpression in S. cerevisiae to investigate the effect on xylose utilization, an increase of about 30% in xylose consumption and about 120% in ethanol production was noted (Traff-Bjerre et al., 2004, Yeast 21:141-150).

Decreasing Xylose Reductase Activity:

A recombinant cell may include one or more genetic modifications that reduce xylose reductase activity. Xylose reductase activity can be reduced by one or more genetic modifications that reduce the expression of or inactivate a gene encoding a xylose reductase.

Decreasing Sensitivity to Catabolite Repression:

Glucose and other sugars, such as galactose or maltose, are able to cause carbon catabolite repression in Crabtree-positive yeast, such as S. cerevisiae. In one study, xylose was found to decrease the derepression of various enzymes of an engineered S. cerevisiae strain capable of xylose utilization by at least 10-fold in the presence of ethanol. Xylose also impaired the derepression of galactokinase and invertase (Belinchon & Gancedo, 2003, Arch. Microbiol. 180:293-297). In certain embodiments, in order to reduce catabolite sensitivity, yeast can include one or more genetic modifications that reduce expression of one or more of GRR1 (SGD accession no. YJR090C), the gene assigned SGD accession no. YLR042C, GAT1 (SGD accession no. YKR067W) and/or one or more genetic modifications that decrease expression of one or more of SNF1 (SGD accession no. YDR477W), SNF4 (SGD accession no. YGL115W), MIG1 (SGD accession no. YGL035C) and CRE1 (SGD accession no. YJL127C). In further embodiments, yeast can include one or more genetic modifications that result in overexpression of the pentose phosphate pathway enzymes. In yet further embodiments, yeast can include one or more genetic modifications that reduce expression of hexo-/glucokinase. In yet a further embodiment, yeast can include one or more genetic modifications that modulate the activity of one or more GATA factors, for example GAT1, DAL80 (SGD accession no. YKR034W), GZF3 (SGD accession no. YJL110C) and GLN3 (SGD accession no. YER040W). The foregoing modifications can be made singly or in combinations of two, three or more modifications.

Increasing Tolerance to Biofuels (e.g., Ethanol), Pathway Intermediates (e.g., Xylitol), Organic Acids and Temperature:

For efficient bioethanol production from lignocellulosic biomass, it is useful to improve cellular tolerance to toxic compounds released during the pretreatment of biomass. In one study, the gene encoding PHO13 (SGD accession no. YDL236W), a protein with alkaline phosphatase activity, was disrupted. This resulted in improved ethanol production from xylose in the presence of three major inhibitors (i.e., acetic acid, formic acid and furfural). Further, the specific ethanol productivity of the mutant in the presence of 90 mM furfural was four fold higher (Fujitomi et al., 2012, Biores. Tech., 111:161-166). Thus, in one embodiment, yeast has one or more genetic modifications that reduce P11013 expression. In other embodiments, yeast, bacterial and fungal cells are evolved under selective conditions to identify strains that can withstand higher temperatures, higher levels of intermediates, higher levels of organic acids and/or higher levels of biofuels (e.g., ethanol). In yet other embodiments, yeast are engineered to reduce expression of FPS1 (SGD accession no. YLL043W); overexpress unsaturated lipid and ergosterol biosynthetic pathways; reduce expression of PHO13 and/or SSK2 (SGD accession no. YNR031C); modulate global transcription factor cAMP receptor protein, through increasing or decreasing expression; increase expression of MSN2 (SGD accession no. YMR037C), RCN1 (SGD accession no. YKL159C), RSA3 (SGD accession no. YLR221C), CDC19 and/or ADH1; or increase expression of Rice ASR1. The foregoing modifications can be made singly or in combinations of two, three or more modifications.

Reducing Production of Byproducts:

Glycerol is one of the main byproducts in C6 ethanol production. Reducing glycerol is desirable for increasing xylose utilization by yeast. Production of glycerol can be reduced by deleting the gene encoding the FPS1 channel protein, which mediates glycerol export, and GPD2 (SGD accession no. YOL059W), which encodes glycerol-3-phosphate dehydrogenase; optionally along with overexpression of GLT1 (SGD accession no. YDL171C) and GLN1 (SGD accession no. YPR035W). In one study, FPS1 and GPD2 were knocked-out in one S. cerevisiae strain, and in another were replaced by overexpression of GLT1 and GLN1, which encode glutamate synthase and glutamine synthetase, respectively. When grown under microaerobic conditions, these strains showed ethanol yield improvements of 13.17% and 6.66%, respectively. Conversely, glycerol, acetic acid and pyruvic acid were found to all decrease, with glycerol down 37.4% and 41.7%, respectively (Zhang and Chen, 2008, Chinese J. Chem. Eng. 16:620-625).

Production of glycerol can also be reduced by deleting the NADH-dependent glycerol-3-phosphate dehydrogenase 1 (GPD1; SGD accession no. YDL022W) and/or the NADPH-dependent glutamate dehydrogenase 1 (GDH1; SGD accession no. YOR375C). Sole deletion of GPD1 or GDH1 reduces glycerol production, and double deletion results in a 46.4% reduction of glycerol production as compared to wild-type S. cerevisiae (Kim et al., 2012, Bioproc. Biosys. Eng. 35:49-54). Deleting FPS1 can decrease production of glycerol for osmoregulatory reasons.

Reducing production of acetate can also increase xylose utilization. Deleting ALD6 (SGD accession no. YPL061W) can decrease production of acetate.

ADH2 can also be deleted to reduce or eliminate acetylaldehyde formation from ethanol and thereby increase ethanol yield.

The foregoing modifications to reduce byproduct formation can be made singly or in combinations of two, three or more modifications.

In addition to ethanol production, a recombinant XI-expressing cell of the disclosure can be suitable for the production of non-ethanolic fermentation products. Such non-ethanolic fermentation products include in principle any bulk or fine chemical that is producible by a eukaryotic microorganism such as a yeast or a filamentous fungus. Such fermentation products may be, for example, butanol, lactic acid, 3-hydroxy-propionic acid, acrylic acid, acetic acid, succinic acid, citric acid, malic acid, fumaric acid, itaconic acid, an amino acid, 1,3-propane-diol, ethylene, glycerol, a β-lactam antibiotic or a cephalosporin. A preferred modified host cell of the disclosure for production of non-ethanolic fermentation products is a host cell that contains a genetic modification that results in decreased alcohol dehydrogenase activity.

Cells expressing the XI polypeptides of the disclosure can be grown under batch, fed-batch or continuous fermentations conditions. Classical batch fermentation is a closed system, wherein the compositions of the medium is set at the beginning of the fermentation and is not subject to artificial alternations during the fermentation. A variation of the batch system is a fed-batch fermentation in which the substrate is added in increments as the fermentation progresses. Fed-batch systems are useful when catabolite repression is likely to inhibit the metabolism of the cells and where it is desirable to have limited amounts of substrate in the medium. Batch and fed-batch fermentations are common and well known in the art. Continuous fermentation is an open system where a defined fermentation medium is added continuously to a bioreactor and an equal amount of conditioned medium is removed simultaneously for processing. Continuous fermentation generally maintains the cultures at a constant high density where cells are primarily in log phase growth. Continuous fermentation systems strive to maintain steady state growth conditions. Methods for modulating nutrients and growth factors for continuous fermentation processes as well as techniques for maximizing the rate of product formation are well known in the art of industrial microbiology.

4.4 Fermentation Methods

A further aspect the disclosure relates to fermentation processes in which the recombinant XI-expressing cells are used for the fermentation of carbon source comprising a source of xylose. Thus, in certain embodiments, the disclosure provides a process for producing a fermentation product by (a) fermenting a medium containing a source of xylose with a recombinant XI-expressing cell as defined herein above, under conditions in which the cell ferments xylose to the fermentation product, and optionally, (b) recovery of the fermentation product. In some embodiments, the fermentation product is an alcohol (e.g., ethanol, butanol, etc.), a fatty alcohol (e.g., a C8-C20 fatty alcohol), a fatty acid (e.g., a C8-C20 fatty acid), lactic acid, 3-hydroxypropionic acid, acrylic acid, acetic acid, succinic acid, citric acid, malic acid, fumaric acid, an amino acid, 1,3-propanediol, itaconic acid, ethylene, glycerol, and a β-lactam antibiotic such as Penicillin G or Penicillin V and fermentative derivatives thereof and cephalosporins. The fermentation process may be an aerobic or an anaerobic fermentation process.

In addition to a source of xylose the carbon source in the fermentation medium may also comprise a source of glucose. The source of xylose or glucose may be xylose or glucose as such or may be any carbohydrate oligo- or polymer comprising xylose or glucose units, such as e.g., lignocellulose, xylans, cellulose, starch and the like. Most microorganisms possess carbon catabolite repression that results in sequential consumption of mixed sugars derived from the lignocellulose, reducing the efficacy of the overall process. To increase the efficiency of fermentation, microorganisms that are capable of simultaneous consumption of mixed sugars (e.g., glucose and xylose) have been developed, for example by rendering them less sensitive to glucose repression (see, e.g., Kim et al., 2010, Appl. Microbiol. Biotechnol. 88:1077-85 and Ho et al., 1999, Adv. Biochem. Eng. Biotechnol. 65:163-92). Such cells can be used for recombinant XI expression and in the fermentation methods of the disclosure.

The fermentation process is preferably run at a temperature that is optimal for the recombinant XI-expressing cells. Thus, for most yeasts or fungal host cells, the fermentation process is performed at a temperature which is less than 38° C., unless temperature tolerant mutant strains are used, in which case the temperature may be higher. For most yeast or filamentous fungal host cells, the fermentation process is suitably performed at a temperature which is lower than 35° C., 33° C., 30° C. or 28° C. Optionally, the temperature is higher than 20° C., 22° C., or 25° C.

An exemplary process is a process for the production of ethanol, whereby the process comprises the steps of: (a) fermenting a medium containing a source of xylose with a transformed host cell as defined above, whereby the host cell ferments xylose to ethanol; and optionally, (b) recovery of the ethanol. The fermentation medium can also comprise a source of glucose that is also fermented to ethanol. The source of xylose can be sugars produced from biomass or agricultural wastes. Many processes for the production of monomeric sugars such as glucose generated from lignocellulose are well known, and are suitable for use herein. In brief, the cellulolytic material may be enzymatically, chemically, and/or physically hydrolyzed to a glucose and xylose containing fraction. Alternatively, the recombinant XI-expressing cells of the disclosure can be further transformed with one or more genes encoding for enzymes effective for hydrolysis of complex substrates such as lignocellulose, and include but are not limited to cellulases, hemicellulases, peroxidases, laccases, chitinases, proteases, and pectinases. The recombinant cells of the disclosure can then be fermented under anaerobic in the presence of glucose and xylose. Where the recombinant cell is a yeast cell, the fermentation techniques and conditions described for example, by Wyman (1994, Biores. Technol. 50:3-16) and Olsson and Hahn-Hagerdal (1996, Enzyme Microb. Technol. 18:312-331) can be used. After completion of the fermentation, the ethanol may be recovered and optionally purified or distilled. Solid residue containing lignin may be discarded or burned as a fuel.

The fermentation process may be run under aerobic and anaerobic conditions. In some embodiments, the process is carried out under microaerobic or oxygen limited conditions. Fermentation can be carried out in a batch, fed-batch, or continuous configuration within (bio)reactors.

5. EXAMPLES

5.1 Materials and Methods

5.1.1 Yeast Culture

Unless stated otherwise for a particular example, yeast transformants were grown in SC-ura media with about 2% glucose at 30° C. for about 24 hours. The media contains approx. 20 g agar, approx. 134 g BD Difco™ Yeast Nitrogen Base without amino acids (BD, Franklin Lakes, N.J., and approx. 2 g SC amino-acid mix containing about 85 mg of the following amino acids unless noted (quantity listed in parentheses): L-Adenine (21.0), L-Alanine, L-Arginine, L-Asparagine, L-Aspartic Acid, L-Cysteine, Glutamine, L-Glutamic Acid, Glycine, L-Histidine, Myo-Inositol, L-Isoleucine, L-Leucine (173.4), L-Lysine, L-Methionine, p-Aminobenzoic Acid (8.6), L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine).

5.1.2 Xylose Isomerase Activity

XI activity in cell lysates was determined using a method based on that of Kersters-Hilderson et al., 1986, Enzyme Microb. Technol. 9:145-148, in which enzymatic conversion of xylose to xylulose by the XI is coupled with the enzymatic conversion of the product (xylulose) to xylitol via the enzyme sorbitol dehydrogenase (SDH). SDH activity requires the oxidation of NADH to NAD⁺. The rate of oxidation of NADH is directly proportional to the rate of SDH conversion of D-xylulose to D-xylitol and is measured by the decrease in absorbance at 340 nm. One unit of enzyme activity as measured by this assay is a decrease of 1 μmole of NADH per minute under assay conditions. All reactions, solutions, plates, and spectrophotometer were equilibrated to about 35° C. prior to use. Assays were performed either on fresh lysates immediately after preparation or lysates that had been frozen at −20° C. immediately after preparation. Assays were performed using a BioTek Model: Synergy H1 Hybrid Reader spectrophotometer and 96-well plates (Corning, Model #Costar® #3598). All spectrophotometric readings were performed at 340 nm. A standard curve of NADH was generated with each assay with concentrations ranging from 0 to about 0.6 mM.

The reaction buffer used for experiments at pH 7.5 was about 100 mM Tris-HCl (pH 7.5). The assay mix was prepared as follows: reaction buffer to which was added about 10 mM MgCl₂, 0.15 mM NADH and 0.05 mg/ml SDH (Roche, catalog #50-720-3313). For experiments where activity was also measured at pH 6, the buffer was changed to about 100 mM sodium phosphate, pH 6. The assay mix for the entire experiment was then prepared as follows: about 10 mM MgCl₂, 1.2 mM NADH and 0.02 mg/ml SDH.

Any sample dilutions were performed using the reaction buffer as diluent. Reactions were set up by aliquotting about 90 μl of assay mix into each well of the plates. About 10 μl of each XI sample was added to the wells. The reactions were started by the addition of about 100 μl substrate solution (about 1 M D-xylose). Reactions were mixed and read immediately using kinetic assay mode for about 10 minutes. Volumetric activity (VA) units are in milli-absorbance (mA) units per minute per ml of lysate added to the reactions (mA/min/ml). Background VA rates of negative control wells (no enzyme added) were subtracted from VA of samples. Determination of fold improvement over positive control (FIOPC) was obtained by dividing the VA of the XI-samples by the VA observed for a control (Orpinomyces xylose isomerase, NCBI:169733248 (Op-XI)) expressed using the same host and expression vector. In some characterizations, the slope of an NADH standard curve was used to convert VA (mA/min) to μmole-NADH/min (or Units). If protein quantitation was performed, specific activities (SA) were calculated where the units for SA are (μmole NADH⁺/min/mg, or U/mg lysate protein). All activities listed (VA or SA) account for any dilutions, volumes of lysate added, and protein concentrations for the lysates assayed.

5.2 Example 2: Activity-Based Discovery Screen for Xylose Isomerases

Libraries used for the activity-based discovery (“ABD”) screen were in the format of excised phagemids. These libraries were constructed as described in U.S. Pat. No. 6,280,926. Sources for these libraries were environmental rumen samples collected from the foregut of deceased herbivores.

An Escherichia coli screening strain was constructed to identify genes from the environmental libraries encoding xylose isomerase activity. Specifically, E. coli strain SEL700, a MG1655 derivative that is recA⁻, phage lambda resistant and contains an F′ plasmid, was complemented with plasmid pJC859, a derivative of pBR322 containing the E. coli recA gene (Kokjohn et al., 1987, J. Bacteriol. 169:1499-1508) to generate a wild-type recA phenotype.

A two-step marker exchange procedure was then used to delete the entire coding sequence of the endogenous xylA xylose isomerase gene. Briefly, pMEV3, a plasmid with a pir-dependent replicon (ori6RK) encoding kanamycin-resistance and the sacB levansucrase, was used as a vector for construction of the xylA deletion plasmid. A fragment of DNA containing the flanking regions of the xylA gene (0.7 kb of sequence 5′ and 0.9 kb of sequence 3′ of xylA) and containing BsaI restriction sites was generated by overlap extension PCR using primers, ligated to pMEV3 digested with BbsI, and transformed into E. coli by electroporation. Clones were confirmed by sequencing, resulting in plasmid pMEV3-ΔxylA (FIG. 1A).

The pMEV3-ΔxylA plasmid was then transformed into strain E. coli strain SEL700 (MG1655 Δ^r, Δ(recA-srl)306,srl-301::Tn10-84(Tets), [F′proAB, lacI^q, ZΔM15, Tn10 (Tet^r)] pJC859). Single-crossover events were selected for by plating on LB agar plates containing kanamycin (final concentration, about 50 μg/ml). After confirmation of integration of pMEV3-ΔxylA on the chromosome, a second crossover event was selected for by growth on LB agar media containing sucrose (FIG. 2). Colonies displaying resistance to kanamycin and the ability to grow on sucrose were screened both by PCR characterization with primers flanking the xylA gene to confirm gene deletion and by growth on a modified MacConkey media (ABD media), comprised of: MacConkey Agar Base (Difco™ #281810) (approximate formula per liter: Pancreatic Digest of Gelatin (17.0 g) Peptones (meat and casein) (3.0 g), Bile Salts No. 3 (1.5 g), Sodium Chloride (5.0 g), Agar (13.5 g), Neutral Red (0.03 g), Crystal Violet (1.0 g, Xylose (30.0 g) and Kanamycin (50 mg). The ABD media contained neutral red, a pH indicator that turns red at a pH<6.8. Colonies of mutants lacking xylA appeared white on this media while colonies with restored xylose metabolism ability appeared red in color due to the fermentation of xylose to xylulose, which lowered the pH of the media surrounding those colonies.

Following the successful deletion of xylA, the resulting strain was cured of pJC859 by the following method: The xylA deletion strain was grown for about 24 hours in LB media containing tetracycline at a final concentration, about 20 μg/ml, at around 37° C. The next day the cells were sub-cultured (1:100 dilution) into LB tetracycline (at the same concentration) media and incubated at about three different temperatures (30, 37, and 42° C.). Cells were passaged the same way as above for about two more days. Dilutions of the resulting cultures were plated on LB plates to isolate single colonies. Colonies were replica plated onto LB agar plates with and without Carbenicillin (at about 100 μg/ml, final concentration). Carbenicillin resistant colonies were deemed to still contain vector pJC859 whereas carbenicillin sensitive colonies were cured of pJC859, restoring the recA genotype of strain SEL700. This strain, SEL700 ΔxylA, was used for the ABD screening.

The ABD screening method was verified by creating a positive control strain by PCR amplification of the xylA gene from E. coli K12 and cloning into the PCR-BluntII TOPO vector (Invitrogen, Carlsbad, Calif.) using standard procedures. This vector (PCR-BluntII-TOPO-xylA, FIG. 1B) was then transformed into the screening strain (SEL700 ΔxylA). Complementation of the xylose phenotype was verified by growth of transformants on ABD media and appearance of red halos indicating xylose utilization.

The libraries were screened for XI activity by infecting strain SEL700 ΔxylA with the excised phagemid libraries. Infected cells were plated onto ABD media and only colonies with red “halos” (indicating xylose fermentation), were carried forward. Positives were purified to single colonies, and regrown on ABD media to confirm phenotype.

5.3 Example 2: Sequence-Based Discovery for Xylose Isomerases

Libraries used for sequence-based discovery (“SBD”) were in the format of genomic DNA (gDNA) extractions. These libraries were constructed as described in U.S. Pat. No. 6,280,926. Sources for these libraries were samples collected from the guts of deceased herbivores.

XI genes often exist in conserved gene clusters (Dodd et al., 2011, Molecular Microbiol. 79:292-304). In order to obtain full length XI gene sequences from metagenomic samples, primers were designed to both upstream and downstream conserved DNA sequences found in several Bacteroides species, typically xylulose kinase and xylose permease, respectively. These flanking DNA sequences were obtained from public databases. Sample genomic DNA was extracted from eleven different animal rumen samples. Left flanking consensus primer has the sequence 5′-GCIGCICARGARGGNATYGTVTT-3′ (SEQ ID NO:177) (this primer codes for the amino acid motif AAQEGIV(F) (SEQ ID NO:178)). Right flanking consensus primer has the sequence 5′-GCDATYTCNGCRATRTACATSGG-3′ (SEQ ID NO:179) (this primer codes for the amino acid motif PMYIAEIA (SEQ ID NO:180)). PCR reactions were carried out using touchdown cycling conditions, and hot start Platinum® Taq DNA polymerase (Invitrogen, Carlsbad, Calif.). PCR products of expected size were purified and subcloned into pCR4-TOPO vector system (Invitrogen, Carlsbad, Calif.). Positive colonies from the TOPO-based PCR libraries were transformed into TOP10 (Invitrogen, Carlsbad, Calif.) and the transformants grown on LB agar plates with kanamycin (about 25 μg/ml final concentration). Resistant colonies were picked and inoculated into 2 columns each of a 96-deep well plate in about 1.2 ml LB kanamycin (25 μg/ml final concentration) media per well. Cultures were grown overnight at about 30° C. The next day plasmids were purified and inserts sequenced. Sequence analysis revealed multiple full length XI genes. Identification of putative ORFs was done by identifying start and stop codons for the longest protein coding region, and subsequent manual curation based on homology to published xylose isomerase DNA sequences.

5.4 Example 3: XI Sequence Analysis

Plasmids from both ABD and SBD screens were purified and vector inserts were sequenced using an ABI 3730x1 DNA Analyzer and ABI BigDye® v3.1 cycle sequencing chemistry. Identification of putative ORFs was done by identifying start and stop codons for the longest protein coding region, and subsequent manual curation based on homology to published xylose isomerase DNA sequences. The XI ORF identified are set forth in Table 2 below, which indicates the sequences and source organism classification for each XI determined from either the ABD or SBD libraries as well as their assigned sequence identifiers. The putative catalytic domains (based on sequence alignments with other XIs) are underlined.

TABLE 2
		Type	SEQ
		of	ID
Clone No.	Class of organism	Sequence	NO:	Sequence
1754MI2_001	Bacteroidales	DNA	1	ATGGCAGTTAAAGAATATTTCCCGGAGATAGGCAAGATCGCCTTTGAAGGAAAGGAGTCC
				AAGAACCCTATGGCATTCCACTACTACAATCCAGAGCAGGTAGTAGCCGGAAAGAAAATG
				AAAGATTGGTTCAAGTTCGCTATGGCATGGTGGCACACCCTCTGCGCTGAAGGTGGCGAC
				CAGTTCGGTCCTGGTACCAAGAAATTCCCTTGGAACACAGGTGCAACTGCACTCGAAAGA
				GCAAAGAACAAAATGGACGCAGGTTTCGAGATCATGAGCAAGCTCGGTATCGAGTATTTC
				TGCTTCCACGATGTTGACCTTATCGACGAGGCTGACACTGTTGAAGAGTACGAGGCTAAC
				ATGAAGGCTATCACAGCTTACGCAAAGGAGAAAATGGCCGCTACTGGCATCAAACTCCTC
				TGGGGAACAGCCAATGTATTCGGCAACAAGAGATATATGAACGGCGCTTCTACCAACCCT
				GACTTCAACGTGGCTGCACGCGCTATGCTCCAGATCAAGAACGCTATCGACGCAACTATC
				GCTCTCGGTGGTGACTGCTATGTATTCTGGGGCGGCCGTGAGGGTTACATGAGCCTTCTC
				AACACCGATATGAAGAGAGAGAAAGAGCACATGGCTACCATGCTTACCATGGCACGCGAC
				TATGCTCGTTCTAAGGGCTTCAAGGGTACCTTCCTTATCGAGCCTAAGCCAATGGAGCCG
				ATGAAGCACCAGTACGATGTCGATACTGAGACTGTCGTAGGTTTCCTCCGCGCCCATGGT
				CTTGACAAGGACTTCAAGGTAAACATCGAGGTTAACCACGCTACTCTCGCAGGCCACACC
				TTCGAGCACGAGCTCCAGTGCGCCGTTGACGCAGGCATGCTCGGAAGCATCGACGCCAAC
				CGTGGTGACTACCAGAACGGCTGGGATACCGACCAGTTCCCTATCGACCTCTATGAGCTC
				GTACAGGCTATGATGGTTATCATCAAGGGCGGCGGTCTCGTCGGCGGTACCAACTTCGAC
				GCCAAGACCCGTCGTAACTCAACAGACCTCGAGGATATCTTCATCGCTCATGTATCCGGC
				ATGGATGTCATGGCACGCGCTCTCCTCATCGCTGCTGACCTTCTCGAGAAATCTCCTATT
				CCTGCAATGGTCAAGGAGCGTTACGCTTCCTACGACTCAGGCATGGGCAAGGACTTCGAG
				AACGGCAAGCTTACTCTCGAGCAGGTTGTCGATTTCGCAAGAAAGAACGGCGAGCCTAAG
				AGCACCAGCGGAAAGCAGGAGCTCTACGAGTCTATCGTCAATCTCTACATCTAA
1754MI2_001	Bacteroidales	Amino	2	MAVKEYFPEIGKIAFEGKESKNPMAFHYYNPEQVVAGKKMKDWFKFAMAWWHTLCAEGGD
		Acid		QFGPGTKKFPWNTGATALERAKNKMDAGFEIMSKLGIEYFCFHDVDLIDEADTVEEYEAN
				MKAITAYAKEKMAATGIKLLWGTANVFGNKRYMNGASTNPDFNVAARAMLQIKNAIDATI
				ALGGDCYVFWGGREGYMSLLNTDMKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEP
				MKHQYDVDTETVVGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELQCAVDAGMLGSIDAN
				RGDYQNGWDTDQFPIDLYELVQAMMVIIKGGGLVGGTNFDAKTRRNSTDLEDIFIAHVSG
				MDVMARALLIAADLLEKSPIPAMVKERYASYDSGMGKDFENGKLTLEQVVDFARKNGEPK
				STSGKQELYESIVNLYI
5586MI6_004	Bacteroidales	DNA	3	ATGGCAAACAAAGAGTACTTCCCGGAGATCGGGAAAATCAAATTCGAAGGCAAGGATTCC
				AAGAACCCGCTTGCATTCCATTATTACAATCCTGAGCAGGTCGTCTGCGGCAAGCCGATG
				AAGGACTGGCTCAAGTTCGCTATGGCATGGTGGCACACCCTCTGCGCAGAGGGTAGCGAC
				CAGTTCGGCGGACCCACCAAGTCATTCCCTTGGAACAAAGCTTCGGATCCCATCGCAAAG
				GCCAAGCAGAAAGTCGACGCCGGTTTCGAGATCATGCAGAAGCTCGGTATCGGATACTAT
				TGCTTCCACGATGTAGACCTCATCGACGAGCCCGCCACCATCGAGGAGTATGAGGCCGAT
				CTCAAGGAGATCGTCGCTTACCTCAAGGAGAAGCAGGCCCAGACCGGCATCAAGCTCCTT
				TGGGGCACCGCCAACGTCTTCGGTCACAAGCGGTACATGAACGGCGCCTCCACCAACCCT
				GATTTCGACGTCGCAGCCCGCGCCATGGTCCAGATCAAGAACGCCATGGACGCCACCATC
				GAGCTCGGCGGCGAGTGCTATGTCTTCTGGGGCGGCCGCGAGGGCTACATGAGCCTCCTC
				AACACCGACATGAAGCGTGAGAAGCAGCATATGGCCACCATGCTCGGCATGGCCCGCGAC
				TATGCACGCGGCAAGGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCG
				ACCAAGCACCAGTATGACGTCGACACCGAGACCGTCATCGGTTTCCTCCGTGCCAACGGT
				CTTGACAAGGACTTCAAGGTCAACATCGAGGTCAATCACGCCACCCTCGCCGGCCACACC
				TTCGAGCATGAGCTCCAGTGCGCCGCCGATGCCGGTCTCCTCGGATCCATCGACGCCAAC
				CGCGGCGACTATCAGAACGGCTGGGATACCGACCAGTTCCCGATCGACCTCTATGAGCTC
				ACCCAGGCCATGATGGTCATCCTCAAGAATGGCGGCCTCGTCGGCGGTACCAACTTCGAC
				GCCAAGACCCGTCGCAACTCCACCGACCTGGACGACATCATCATCGCCCACGTCAGCGGT
				ATGGACATCATGGCACGCGCACTCCTCGTCGCTGCCGACGTCCTCACCAAGTCCGAGCTT
				CCCAAGATGCTCAAGGAGCGTTACGCTTCCTTCGACTCCGGCAAGGGCAAGGAGTTCGAA
				GAGGGCAAGCTCACTCTCGAGCAGGTCGTAGAGTACGCCAAGACCAAGGGCGAGCCCAAG
				GCCACCAGCGGCAAGCAGGAGCTCTACGAGACCATCGTCAACATGTACATCTAA
5586MI6_004	Bacteroidales	Amino	4	MANKEYFPEIGKIKFEGKDSKNPLAFHYYNPEQVVCGKPMKDWLKFAMAWWHTLCAEGSD
		Acid		QFGGPTKSFPWNKASDPIAKAKQKVDAGFEIMQKLGIGYYCFHDVDLIDEPATIEEYEAD
				LKEIVAYLKEKQAQTGIKLLWGTANVFGHKRYMNGASTNPDFDVAARAMVQIKNAMDATI
				ELGGECYVFWGGREGYMSLLNTDMKREKQHMATMLGMARDYARGKGFKGTFLIEPKPMEP
				TKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELQCAADAGLLGSIDAN
				RGDYQNGWDTDQFPIDLYELTQAMMVILKNGGLVGGTNFDAKTRRNSTDLDDIIIAHVSG
				MDIMARALLVAADVLTKSELPKMLKERYASFDSGKGKEFEEGKLTLEQVVEYAKTKGEPK
				ATSGKQELYETIVNMYI
5749MI1_003	Bacteroidales	DNA	5	ATGAATTTTTATAAAGGCGAAAAAGAATTCTTCCCCGGAATAGGAAAGATTCAGTTTGAA
				GGACGCGAGTCAAAGAACCCGATGGCGTTTCATTATTATGACGAAAACAAGGTGGTGATG
				GGTAAAACACTGAAGGATCATCTTCGTTTTGCAATGGCTTACTGGCATACGCTTTGTGCC
				GAAGGGGGCGACCAGTTTGGCGGTGGTACGAAAACATTCCCCTGGAATGCTGCTGCCGAC
				CCGATCAGCCGTGCCAAATATAAGATGGATGCAGCGTTCGAGTTTATGACAAAATGCAGC
				ATCCCTTATTACTGTTTCCATGATGTGGACGTGGTGGACGAAGCTCCCACGCTGGCTCAG
				TTTGAAAAAGACCTTCATACGATGGTAGGCCATGCCAAAGGGCTTCAGCAGGCAACCGGA
				AAAAAACTGTTATGGTCTACTGCCAACGTGTTCAGCAACAAACGCTATATGAACGGGGCT
				GCCACTAATCCTGACTTCTCGGCCGTGGCTTGTGCCGGTACGCAGATCAAGAATGCGATC
				GATGCCTGTATCGCGCTGGACGGTGAAAACTATGTGTTCTGGGGCGGACGTGAAGGATAT
				ATGGGCTTGCTCAATACCGATATGAAACGCGAAAAAGACCATCTGGCCATGATGCTGACG
				ATGGCACGCGACTATGGCCGCAAGAACGGTTTCAAAGGTACTTTCCTGATCGAGCCGAAA
				CCGATGGAACCGACCAAGCATCAATATGATGTCGACTCGGAAACTGTAATCGGCTTCCTA
				CGTCATTATGGCCTGGATAAAGACTTCGCCCTGAATATCGAAGTAAATCATGCAACCCTG
				GCCGGACATACGTTCGAGCACGAATTGCAGGCTGCTGTCGATGCCGGTATGCTGTGCAGT
				ATCGATGCCAACCGTGGTGACTACCAGAATGGCTGGGATACCGACCAATTCCCGATGGAC
				ATCTACGAACTGACTCAGGCTTGGCTGGTCATTCTGCAAGGTGGTGGTCTGACAACCGGC
				GGAACGAACTTCGATGCCAAGACCCGCCGCAACTCGACCGACCTGGACGATATCTTCCTG
				GCTCATATAGGTGGTATGGATGCGTTTGCCCGTGCCCTGATCACGGCTGCTGCCATCCTT
				GAAAACTCCGATTACACGAAGATGCGTGCCGAACGTTACACCAGCTTCGATGGTGGCGAA
				GGCAAAGCGTTTGAAGACGGTAAACTTTCTCTGGAAGACCTGCGTACGATCGCTCTCCGC
				GACGGAGAACCGAAGATGGTCAGCGGCAAACAGGAATTATATGAGATGATTCTCAATTTA
				TACATATAA
5749MI1_003	Bacteroidales	Amino	6	MNFYKGEKEFFPGIGKIQFEGRESKNPMAFHYYDENKVVMGKTLKDHLRFAMAYWHTLCA
		Acid		EGGDQFGGGTKTFPWNAAADPISRAKYKMDAAFEFMTKCSIPYYCFHDVDVVDEAPTLAQ
				FEKDLHTMVGHAKGLQQATGKKLLWSTANVFSNKRYMNGAATNPDFSAVACAGTQIKNAI
				DACIALDGENYVFWGGREGYMGLLNTDMKREKDHLAMMLTMARDYGRKNGFKGTFLIEPK
				PMEPTKHQYDVDSETVIGFLRHYGLDKDFALNIEVNHATLAGHTFEHELQAAVDAGMLCS
				IDANRGDYQNGWDTDQFPMDIYELTQAWLVILQGGGLTTGGTNFDAKTRRNSTDLDDIFL
				AHIGGMDAFARALITAAAILENSDYTKMRAERYTSFDGGEGKAFEDGKLSLEDLRTIALR
				DGEPKMVSGKQELYEMILNLYI
5750MI1_003	Bacteroidales	DNA	7	ATGAATTACTTTAAAGGTGAGAAAGAGTTCTTCCCGGGAATCGGGAAAATAGAGTTTGAA
				GGACGTGAATCGAAGAATCCGATGGCTTTTCATTACTATGACGAGAACAAGGTTGTCATG
				GGGAAGACCTTGAAGGACCATCTGCGTTTTGCGATGGCTTATTGGCATACGCTGTGTGCG
				GAAGGCGCCGACCAGTTCGGCGGCGGGACGAAGGCATTTCCCTGGAATACCGGGGCGGAT
				CGTATTTCCCGTGCCAAGTATAAGATGGATGCTGCTTTTGAGTTTATGACGAAATGTAAC
				ATCCCGTACTATTGTTTCCATGATGTGGATGTGGTGGATGAAGCTCCGACACTGGCCGAA
				TTTGAAAAAGACTTGCATACGATGGTCGAATATGCCAAGCAGCATCAGGAGGCAACCGGG
				AAAAAACTGTTGTGGTCTACCGCCAATGTGTTCAGCAATAAACGTTATATGAACGGGGCT
				GCCACAAATCCGTATTTCCCTGCTGTCGCTTGTGCGGGTACGCAGATCAAGAATGCTATC
				GACGCTTGTATTGCCCTGGGCGGCGAAAACTATGTGTTCTGGGGCGGTCGTGAAGGGTAT
				ATGAGCTTGTTGAACACCAATATGAAACGCGAAAAGGAACATCTCGCCATGATGTTGACG
				ATGGCTCGCGATTATGCGCGTAAGAACGGCTTCAAAGGTACTTTCCTGGTAGAGCCTAAA
				CCGATGGAACCGACCAAACATCAGTATGATGTGGACACAGAAACTGTTATCGGCTTCCTG
				CGTCATTACGGCCTTGACAAGGACTTTGCCATCAACATCGAAGTGAATCATGCTACATTG
				GCTGGACATACATTCGAACATGAGCTTCAGGCGGCTGCCGATGCCGGTATGCTGTGCAGC
				ATCGACGCCAACCGCGGCGATTACCAGAATGGTTGGGACACGGATCAGTTCCCGGTCGAC
				ATCTACGAACTGACACAGGCGTGGCTGGTTATCCTCGAAGCGGGTGGCCTGACTACCGGT
				GGTACGAACTTCGACGCCAAGACGCGCCGCAACTCGACTGACCTGGACGATATCTTCCTG
				GCACACATCGGTGGTATGGATTCGTTTGCCCGTGCTTTGATGGCGGCTGCCGATATATTG
				GAACACTCCGATTACAAAAAGATGCGTGCCGAACGTTATGCCAGCTTCGATCAAGGCGAC
				GGCAAGAAGTTCGAAGATGGTAAACTCCTTCTCGAGGACCTCCGCACCATCGCTCTTGCC
				TCCGGCGAACCGAAGCAAATCAGCGGGAAACAGGAATTGTATGAAATGATTATCAACCAG
				TACATTTAA
5750MI1_003	Bacteroidales	Amino	8	MNYFKGEKEFFPGIGKIEFEGRESKNPMAFHYYDENKVVMGKTLKDHLRFAMAYWHTLCA
		Acid		EGADQFGGGTKAFPWNTGADRISRAKYKMDAAFEFMTKCNIPYYCFHDVDVVDEAPTLAE
				FEKDLHTMVEYAKQHQEATGKKLLWSTANVFSNKRYMNGAATNPYFPAVACAGTQIKNAI
				DACIALGGENYVFWGGREGYMSLLNTNMKREKEHLAMMLTMARDYARKNGFKGTFLVEPK
				PMEPTKHQYDVDTETVIGFLRHYGLDKDFAINIEVNHATLAGHTFEHELQAAADAGMLCS
				IDANRGDYQNGWDTDQFPVDIYELTQAWLVILEAGGLTTGGTNFDAKTRRNSTDLDDIFL
				AHIGGMDSFARALMAAADILEHSDYKKMRAERYASFDQGDGKKFEDGKLLLEDLRTIALA
				SGEPKQISGKQELYEMIINQYI
5750MI2_003	Bacteroidales	DNA	9	ATGAATTATTTTAAAGGTGAAAAAGAGTTTTTCCCTGGAATCGGGAAAATAGAGTTTGAA
				GGACGTGAGTCGAAGAATCCGATGGCTTTTCATTATTATGATGAAAACAAGGTCGTAATG
				GGCAAGACCTTGAAAGATCACCTCCGCTTTGCAATGGCTTACTGGCATACGTTGTGCGCG
				GAAGGCGCAGACCAGTTTGGCGGTGGCACAAAATCATTCCCCTGGAATACCGCAGCGGAT
				CGTATTTCCCGCGCTAAATATAAAATGGATGCTGCTTTCGAGTTTATGACCAAGTGCAGT
				ATCCCGTACTATTGTTTCCATGATGTGGACGTGGTGGACGAAGCTCCGGCACTGGCCGAA
				TTTGAAAAGGACCTGCATACGATGGTGGGATTCGCCAAACAACACCAGGAAGCAACCGGA
				AAGAAACTGTTGTGGTCTACAGCCAATGTATTCGGGCATAAACGTTATATGAACGGAGCG
				GCTACCAATCCTTATTTCCCGGCTGTCGCTTGTGCCGGTACGCAGATCAAGAATGCAATC
				GACGCCTGTATCGAGCTGGGTGGAGAGAACTATGTATTCTGGGGCGGACGCGAAGGCTAC
				ATGAGCCTGCTGAACACCAATATGAAACGTGAAAAGGATCATTTGGCCATGATGCTGACA
				ATGGCACGCGATTATGCCCGCAAGAATGGTTTCAAGGGTACTTTCCTGGTGGAATCTAAG
				CCGATGGAACCGACCAAACATCAGTATGACGCAGATACGGAAACCGTGATCGGCTTCCTG
				CGCCACTATGGCCTCGACAAGGATTTCGCTATCAACATTGAAGTGAACCATGCTACATTG
				GCCGGCCATACATTCGAACATGAACTTCAGGCTGCTGCCGATGCCGGTATGCTGTGCAGC
				ATCGATGCAAATAGAGGCGACTATCAGAATGGTTGGGATACGGATCAGTTCCCCGTAGAC
				ATTTACGAACTGACACAGGCCTGGCTGGTTATCCTGGAAGCGGGCGGACTGACAACCGGA
				GGTACGAACTTCGATGCGAAGACCCGTCGTAACTCGACTGACCTCGACGATATCTTCCTG
				GCCCATATCGGCGGTATGGATTCGTTTGCACGTGCCTTGATGGCAGCTGCCGATATCCTG
				GAACATTCTGATTACAAGAAGATGCGTGCCGAACGTTACGCCAGCTTCGACCAGGGCGAC
				GGCAAGAAGTTCGAAGACGGCAAACTCCTTCTCGAAGACCTGCGCACAATTGCCCTTGCC
				GGCGACGAACCGAAGCAGATCAGCGGCAAGCAGGAGTTGTATGAGATGATTATCAATCAG
				TATATTTAA
5750MI2_003	Bacteroidales	Amino	10	MNYFKGEKEFFPGIGKIEFEGRESKNPMAFHYYDENKVVMGKTLKDHLRFAMAYWHTLCA
		Acid		EGADQFGGGTKSFPWNTAADRISRAKYKMDAAFEFMTKCSIPYYCFHDVDVVDEAPALAE
				FEKDLHTMVGFAKQHQEATGKKLLWSTANVFGHKRYMNGAATNPYFPAVACAGTQIKNAI
				DACIELGGENYVFWGGREGYMSLLNTNMKREKDHLAMMLTMARDYARKNGFKGTFLVESK
				PMEPTKHQYDADTETVIGFLRHYGLDKDFAINIEVNHATLAGHTFEHELQAAADAGMLCS
				IDANRGDYQNGWDTDQFPVDIYELTQAWLVILEAGGLTTGGTNFDAKTRRNSTDLDDIFL
				AHIGGMDSFARALMAAADILEHSDYKKMRAERYASFDQGDGKKFEDGKLLLEDLRTIALA
				GDEPKQISGKQELYEMIINQYI
5586MI5_004	Bacteroides	DNA	11	ATGAAACAGTATTTCCCGAACATCTCCGCCATCAAGTTTGAGGGCGTCGAGAGCAAGAAT
				CCCCTGGCTTACCGCTACTACGACCGCGACCGCGTCGTCATGGGTAAGAAGATGAGCGAA
				TGGTTTAAGTTCGCTATGTGCTGGTGGCACACCCTCTGCGCCGAGGGCTCCGATCAGTTC
				GGTCCCGGCACAAAGACCTTCCCCTGGAACGCCGCCGCCGACCCCGTGCAGGCTGCCAAG
				GACAAGGCCGACGCTGGCTTCGAGATCATGCAGAAACTCGGCATCGAGTACTACTGCTTC
				CACGACGTTGACCTCGTGGCCGAGGCTCCCGACGTGGAGACCTACGAGAAGAACCTCAAG
				GAGATCGTGGCTTATCTCAAGCAGAAACAGGCTGAGACGGGCATCAAGCTGCTCTGGGGC
				ACTGCCAACGTCTTCGGACACAAGCGCTACATGAACGGAGCCTCCACGAACCCCGACTTC
				GATGTCGTGGCACGCGCTATCGTGCAGATCAAGAACGCCATCGATGCTACCATCGAGCTG
				GGCGGCACCAACTACGTCTTCTGGGGCGGTCGCGAAGGCTACATGAGCCTGCTCAACACC
				GATATGAAGCGCGAGAAGGAGCACATGGCTACGATGTTGACGATGGCACGCGACTATGCC
				CGTTCTAAGGGATTCAAGGGCACGTTCCTCATCGAACCCAAACCCATGGAACCCACGAAG
				CATCAGTACGATGCGGACACCGAGACGGTCATCGGATTCCTCCGTGCTCATGGTCTCGAC
				AAGGATTTCAAGGTCAACATCGAGGTCAACCACGCCACGCTGGCCGGACACACGTTCGAG
				CATGAGCTGGCCTGCGCCGTAGACGCCGATATGCTCGGCAGCATCGATGCCAATCGCGGC
				GACTATCAGAACGGATGGGACACCGACCAGTTCCCCATCGACCACTACGAACTCACGCAG
				GCTATGCTGCAGATCATCCGCAACGGAGGTTTCAAGGACGGTGGCACCAATTTTGACGCT
				AAGACGCGCCGCAACAGCACCGACCTCGAGGATATCTTCATCGCTCACGTAGCAGCCATG
				GACGCCATGGCCCACGCCCTGTTGTCGGCTGCCGATATCATCGAGAAGTCGCCCATCTGC
				ACGATGGTCAAGGAGCGTTACGCCAGCTTCGATGCCGGCGAAGGCAAGCGCTTCGAAGAA
				GGCAAGATGACCCTCGAGGAAGCCTACGAGTATGGCAAGAAGGTCGGGGAGCCCAAGCAG
				ACCAGCGGAAAGCAGGAGCTCTACGAAGCCATTGTCAATATGTATTGA
5586MI5_004	Bacteroides	Amino	12	MKQYFPNISAIKFEGVESKNPLAYRYYDRDRVVMGKKMSEWFKFAMCWWHTLCAEGSDQF
		Acid		GPGTKTFPWNAAADPVQAAKDKADAGFEIMQKLGIEYYCFHDVDLVAEAPDVETYEKNLK
				EIVAYLKQKQAETGIKLLWGTANVFGHKRYMNGASTNPDFDVVARAIVQIKNAIDATIEL
				GGTNYVFWGGREGYMSLLNTDMKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEPTK
				HQYDADTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDADMLGSIDANRG
				DYQNGWDTDQFPIDHYELTQAMLQIIRNGGFKDGGTNFDAKTRRNSTDLEDIFIAHVAAM
				DAMAHALLSAADIIEKSPICTMVKERYASFDAGEGKRFEEGKMTLEEAYEYGKKVGEPKQ
				TSGKQELYEAIVNMY
5586MI202_004	Bacteroides	DNA	13	ATGGCAACAAAAGAGTATTTTCCCGGAATAGGAAAGATTAAATTCGAAGGTAAAGAGAGT
				ATGAACCCGATGGCATATCGTTACTACGATGCTGAGAAGGTAATCATGGGTAAGAAGATG
				AAAGATTGGTTGAAGTTTGCTATGGCTTGGTGGCACACTCTCTGCGCAGAAGGTGGTGAC
				CAATTCGGTGGCGGAACGAAACAATTCCCTTGGAATGGTGACTCTGACGCTTTGCAAGCA
				GCTAAAAATAAATTGGATGCAGGTTTCGAATTCATGCAGAAGATGGGTATCGAATACTAT
				TGCTTCCACGATGTAGACCTGATTTCTGAAGGTGCAAGCATCGAAGAATACGAAGCTAAC
				TTGAAAGCTATCGTAGCTTATGCAAAAGAAAAACAGGCTGAAACTGGTATCAAGCTGTTG
				TGGGGTACTGCTAACGTATTCGGTCATGCACGTTATATGAACGGTGCTGCTACCAATCCT
				GATTTCGACGTTGTAGCACGCGCTGCTGTTCAGATCAAGAACGCTATTGACGCTACTATC
				GAACTGGGTGGTTCAAACTATGTATTCTGGGGCGGTCGCGAAGGTTACATGTCTTTGCTG
				AACACTGACCAGAAACGTGAAAAAGAACACCTTGCAAAGATGTTGACTATCGCTCGTGAC
				TATGCACGTGCTCGTGGCTTCAAAGGTACTTTCCTGATTGAGCCGAAACCGATGGAACCG
				ACAAAACATCAGTATGATGTAGATACTGAAACAGTTATCGGCTTCCTGAAAGCTCACGGT
				TTGGATAAGGATTTCAAAGTAAACATCGAGGTTAATCACGCAACTTTGGCTGGCCATACT
				TTCGAACACGAACTGGCTGTAGCTGTTGACAACGGCATGTTAGGTTCTATCGACGCTAAC
				CGTGGTGACTACCAGAACGGTTGGGATACTGACCAATTCCCTATCGATAACTACGAACTG
				ACTCAAGCTATGATGCAGATCATCCGCAACGGTGGTTTGGGTAATGGCGGTACTAACTTC
				GACGCTAAGACCCGTCGTAACTCTACCGACCTGGAAGATATCTTCATCGCTCACATTGCA
				GGTATGGATGCTATGGCACGTGCTCTGGAAAGTGCAGCTAAATTACTGGAAGAATCTCCT
				TATAAGAAAATGTTGGCTGATCGTTACGCATCATTCGACGGTGGCAAGGGTAAGGAATTC
				GAAGAAGGCAAATTGTCTTTGGAAGATGTTGTAGCTTATGCGAAAGCTAACGGCGAACCG
				AAGCAAACCAGCGGCAAGCAAGAATTGTATGAAGCAATCGTGAATATGTATTGCTAA
5586MI202_004	Bacteroides	Amino	14	MATKEYFPGIGKIKFEGKESMNPMAYRYYDAEKVIMGKKMKDWLKFAMAWWHTLCAEGGD
		Acid		QFGGGTKQFPWNGDSDALQAAKNKLDAGFEFMQKMGIEYYCFHDVDLISEGASIEEYEAN
				LKAIVAYAKEKQAETGIKLLWGTANVFGHARYMNGAATNPDFDVVARAAVQIKNAIDATI
				ELGGSNYVFWGGREGYMSLLNTDQKREKEHLAKMLTIARDYARARGFKGTFLIEPKPMEP
				TKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDAN
				RGDYQNGWDTDQFPIDNYELTQAMMQIIRNGGLGNGGTNFDAKTRRNSTDLEDIFIAHIA
				GMDAMARALESAAKLLEESPYKKMLADRYASFDGGKGKEFEEGKLSLEDVVAYAKANGEP
				KQTSGKQELYEAIVNMYC
5586MI211_003	Bacteroides	DNA	15	ATGGCAAAAGAGTATTTTCCTGGCGTGAAAAAAATCCAGTTCGAGGGTAAGGACAGTAAG
				AATCCAATGGCTTACCGTTATTATGATGCAGAGAAGGTCATCATGGGTAAGAAGATGAAG
				GATTGGTTGAAGTTCGCTATGGCTTGGTGGCACACTTTGTGCGCTGAGGGCGCAGACCAG
				TTCGGTGGCGGTACTAAGACTTTCCCTTGGAACGAAGGTGCAAACGCTTTGGAAGTTGCT
				AAGAATAAGGCTGATGCTGGTTTCGAGATTATGGAGAAGCTTGGCATCGAGTACTACTGT
				TTCCACGATGTAGACCTCGTTGAGGAGGCTGCAACTATCGAGGAGTATGAGGCTAACATG
				AAGGCTATCGTTGCTTATCTTAAGGAGAAGCAGGCTGCTACTGGCAAGAAGCTTCTTTGG
				GGTACTGCTAACGTATTCGGCAACAAGCGCTATATGAACGGTGCTTCTACAAACCCTGAC
				TTCGACGTTGTTGCTCGCGCTTGTGTTCAGATTAAGAACGCTATCGACGCTACTATCGAA
				CTTGGTGGTACAAACTACGTATTCTGGGGTGGCCGCGAGGGTTATATGAGCCTTCTTAAC
				ACAGATATGAAGCGTGAGAAGGAGCACATGGCAACTATGCTTACTAAGGCTCGCGACTAC
				GCTCGTTCAAAGGGCTTTACTGGTACATTCCTTATCGAGCCAAAGCCAATGGAACCATCA
				AAGCATCAGTATGATGTTGATACTGAGACTGTTTGTGGTTTCTTGAGGGCTCACGGTCTT
				GACAAGGACTTCAAGGTAAACATCGAGGTTAACCACGCTACTTTGGCTGGTCACACATTC
				GAGCACGAGTTGGCTGCTGCTGTTGATAACGGTATGCTTGGCTCTATCGACGCTAACCGC
				GGTGACTACCAGAACGGTTGGGATACTGACCAGTTCCCTATCGACAACTTCGAGCTTATT
				CAGGCTATGATGCAGATTATCCGCAACGGTGGTCTTGGCAACGGTGGTACAAACTTCGAC
				GCTAAGACTCGTCGTAACTCAACTGACCTTGAGGATATCTTCATCGCACACATCGCTGGT
				ATGGATGCAATGGCTCGCGCTCTTGAGAACGCAGCAGACCTTTTGGAGAACTCTCCAATC
				AAGAAGATGGTTGCTGAGCGTTACGCTTCATTCGACAGCGGCAAGGGTAAGGAGTTCGAG
				GAAGGCAAGTTGAGCCTTGGGGACATCGTTGCTTATGCTAAGCAGAACGGTGAGCCTAAG
				CAGACAAGCGGTAAGCAGGAGCTTTACGAGGCTATCGTAAACATGTACTGCTAA
5586MI211_003	Bacteroides	Amino	16	MAKEYFPGVKKIQFEGKDSKNPMAYRYYDAEKVIMGKKMKDWLKFAMAWWHTLCAEGADQ
		Acid		FGGGTKTFPWNEGANALEVAKNKADAGFEIMEKLGIEYYCFHDVDLVEEAATIEEYEANM
				KAIVAYLKEKQAATGKKLLWGTANVFGNKRYMNGASTNPDFDVVARACVQIKNAIDATIE
				LGGTNYVFWGGREGYMSLLNTDMKREKEHMATMLTKARDYARSKGFTGTFLIEPKPMEPS
				KHQYDVDTETVCGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELAAAVDNGMLGSIDANR
				GDYQNGWDTDQFPIDNFELIQAMMQIIRNGGLGNGGTNFDAKTRRNSTDLEDIFIAHIAG
				MDAMARALENAADLLENSPIKKMVAERYASFDSGKGKEFEEGKLSLGDIVAYAKQNGEPK
				QTSGKQELYEAIVNMYC
5606MI1_005	Bacteroides	DNA	17	ATGGCGACAAAAGAATACTTTCCCGGAATAGGGAAAATCAAGTTTGAGGGTGTGAATAGC
				TATAATCCGCTGGCATACAGATATTACGATGCCGAGCGCATAGTCCTTGGCAAGCCGATG
				AAGGAGTGGCTCAAGTTTGCCATGGCATGGTGGCACACACTCTGCGCAGAGGGTGGCGAC
				CAGTTTGGCGGCGGTACGAAGAATTTTCCCTGGAATGGAGATCCCGATCCGGTACAGGCC
				GCAAAAAACAAAGTAGACGCCGGCTTCGAATTCATGACCAAGATGGGAATAGAGTATTTC
				TGTTTCCACGACGTGGATCTCGTCAGCGAGGCAGCAACCATCGAGGAGTATGAGGCCAAC
				CTGAAGGAAGTGGTGGGCTACATCAAGGAAAAGCAGGCCGAGACGGGGATCAAAAACCTC
				TGGGGCACTGCCAACGTGTTCAGCCACGCGCGCTACATGAACGGAGCCGCCACCAACCCC
				GACTTCGATGTAGTGGCCCGCGCAGCCGTGCAGATCAAGAATGCTATCGACGCCACGATA
				GCCTTAGGTGGCACCAACTACGTGTTCTGGGGTGGCCGTGAAGGTTACATGAGCCTGCTC
				AACACCGACCAGAAGCGCGAGAAGGAGCATCTGGCAATGATGCTCCGCATGGCCCGCGAC
				TATGCGCGTGCAAAAGGCTTCACCGGCACCTTCCTTATCGAGCCCAAGCCGATGGAGCCC
				ACCAAGCACCAGTATGATGTAGACACCGAGACTGTGATAGGCTTCCTCCGTGCCCACGGC
				CTCGACAAGGACTTCAAGGTCAACATAGAGGTGAACCACGCCACCCTGGCCGGCCATACC
				TTCGAGCATGAGCTGGCAGTGGCCGTGGACAACGGTATGCTCGGCAGCATCGACGCCAAC
				CGCGGTGACTACCAGAACGGCTGGGATACCGACCAGTTCCCCATCGACAACTACGAGCTG
				ACCCAGGCCATGATGCAGATAATACGCAACGGCGGCTTCGGCAACGGCGGATGCAACTTC
				GACGCCAAGACACGCCGCAACTCCACCGACCTGGAGGATATCTTCATAGCCCACATAGCA
				GGCATGGACGCCATGGCCCGCGCCCTGCTCAGCGCAGCAGAAGTGCTGGAGAAATCGCCC
				TACAGGAAGATGCTCGCCGAGCGCTACGCACCGTTTGATGCCGGCCAGGGAAAGGCATTT
				GAAGAGGGCGCAATGTCGCTCACCGACCTTGTGGAGTATGCCAAGGAGCATGGCGAGCCC
				ACACAGACTTCCGGCAAGCAGGAACTCTATGAGGCAATCGTCAATATGTATTGCTAA
5606MI1_005	Bacteroides	Amino	18	MATKEYFPGIGKIKFEGVNSYNPLAYRYYDAERIVLGKPMKEWLKFAMAWWHTLCAEGGD
		Acid		QFGGGTKNFPWNGDPDPVQAAKNKVDAGFEFMTKMGIEYFCFHDVDLVSEAATIEEYEAN
				LKEVVGYIKEKQAETGIKNLWGTANVFSHARYMNGAATNPDFDVVARAAVQIKNAIDATI
				ALGGTNYVFWGGREGYMSLLNTDQKREKEHLAMMLRMARDYARAKGFTGTFLIEPKPMEP
				TKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDAN
				RGDYQNGWDTDQFPIDNYELTQAMMQIIRNGGFGNGGCNFDAKTRRNSTDLEDIFIAHIA
				GMDAMARALLSAAEVLEKSPYRKMLAERYAPFDAGQGKAFEEGAMSLTDLVEYAKEHGEP
				TQTSGKQELYEAIVNMYC
5606MI2_003	Bacteroides	DNA	19	ATGGCAACAAAGGAATATTTTCCCCATATAGGGAAGATCCAGTTCAAAGGCACGGAATCG
				TACGATCCGATGTCGTATCGTTACTATGACGCCGAGCGCGTAGTTCTGGGCAAGCCCATG
				AAGGAATGGCTGAAATTCGCCATGGCATGGTGGCACACATTGTGCGCCGAGGGCGGCGAC
				CAGTTCGGCGGCGGAACGAAGAAGTTCCCCTGGAACGAGGGCGAGGACGCCATGACCATC
				GCCAAGCAGAAGGCTGACGCCGGCTTCGAGATCATGCAGAAGCTCGGCATCGAGTATTTC
				TGCTTCCACGACATCGACCTGATCGGCGACCTGGGCGACGACATCGAGGACTATGAGAAC
				CGTATGCACGAAATCACCGCACACCTGAAGGAGAAGATGGCCGCCACGGGCATCAAGAAC
				CTGTGGGGCACTGCCAACGTGTTCGGCCACGCACGCTATATGAACGGCGCCGCCACCAAC
				CCCGACTTCGACGTTGTGGCACGCGCATGTGTGCAGATCAAGAACGCCATCGACGCCACC
				ATCGCTCTAGGCGGTACAAACTATGTATTCTGGGGCGGCCGCGAGGGCTACATGAGCCTG
				CTGAACACCGACCAGAAGCGCGAGAAAGAGCACTTGGCTACCATGCTGACCATGGCACGC
				GACTATGCCCGCGCCAATGGCTTCACCGGAACGTTCCTGATCGAGCCCAAACCCATGGAG
				CCCAGCAAGCATCAGTATGATGTGGATACCGAGACCGTAATCGGCTTCCTGAAGGCCCAC
				AACCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCATGCCACTCTGGCCGGCCAC
				ACATTCGAGCATGAGCTGGCAGTAGCCGTGGACAACGGCATGCTGGGCAGCATCGACGCC
				AACCGCGGCGACTATCAGAACGGCTGGGACACCGACCAGTTCCCCATCGACAACTATGAG
				CTGACCCAGGCCATGATGCAGATAATCCGCAACGGTGGCCTCGGCAACGGCGGTACCAAC
				TTCGACGCCAAGACACGTCGCAACTCCACCGACCTGGACGACATCTTCATCGCTCACATC
				GCCGGTATGGACGCTATGGCCCGCGCTCCGCTCAGCGCAGCCGACGTGCTTGAGAAGTCG
				CCTTACAAGAAGATGCTGGCCGACCGCTACGCTTCATTCGACAGCGGCGAGGGCAAGAAG
				TTCGAGGAAGGCAAGATGACTCTGGAGGATGTCGTGGCCTACGCCAAGAAGAATCCCGAA
				CCCGCTCAGACCAGCGGCAAGCAGGAACTCTACGAGGCCATCATCAACATGTACGCCTGA
5606MI2_003	Bacteroides	Amino	20	MATKEYFPHIGKIQFKGTESYDPMSYRYYDAERVVLGKPMKEWLKFAMAWWHTLCAEGGD
		Acid		QFGGGTKKFPWNEGEDANTIAKQKADAGFEIMQKLGIEYFCFHDIDLIGDLGDDIEDYEN
				RMHEITAHLKEKMAATGIKNLWGTANVFGHARYMNGAATNPDFDVVARACVQIKNAIDAT
				IALGGTNYVFWGGREGYMSLLNTDQKREKEHLATMLTMARDYARANGFTGTFLIEPKPME
				PSKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDA
				NRGDYQNGWDTDQFPIDNYELTQAMMQIIRNGGLGNGGTNFDAKTRRNSTDLDDIFIAHI
				AGMDAMARAPLSAADVLEKSPYKKMLADRYASFDSGEGKKFEEGKMTLEDVVAYAKKNPE
				PAQTSGKQELYEAIINMYA
5610MI3_003	Bacteroides	DNA	21	ATGGCAACAAAAGAATTTTTTCCCGAGATTGGTAAAATCAAGTTTGAGGGCCGCGAAAGC
				CGCAATCCCCTCGCATTCCGCTACTACGGCCCCGAGAAAGTCGTTCTTGGCAAGAAGATG
				AAAGACTGGTTCAAGTTTGCGATGGCTTGGTGGCACACACTGTGCGCCCAGGGCACCGAC
				CAGTTTGGTGGCGACACCAAGCAGTTTCCGTGGAACACTGCCAGTGACCCCATGCAGGCC
				GCCAAGGATAAGGTGGATGCCGGATTTGAATTCATGACCAAGATGGGCATTGAGTACTTC
				TGCTTCCACGATGTGGATCTCGTCGCCGAGGCCGCCACTGTCGAGGAGTATGAGGCTAAC
				CTCAAGACCATCGTCGCCTACATCAAAGAGAAACAAGCCGAGACCGGCATCAAGAACCTG
				TGGGGCACAGCCAACGTATTCGGACACAAACGCTACATGAACGGTGCCGCCACCAACCCC
				GACTTTGATGTCGTGGCACGCGCCATCGTGCAAATCAAGAACGCCATCGACGCCACCATC
				GAGTTGGGCGGCACGAGTTACGTCTTTTGGGGCGGCCGCGAGGGCCACATGAGCCTGCTC
				AACACCGACCAGAAGCGCGAGAAGGAGCACCTTGCACGCATGCTGACCATGGCACGCGAC
				TATGCCCGCGCACGTGGTTTCAACGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCG
				ACCAAGCACCAATATGATGTGGACACCGAGACCGTCATCGGTTTCCTGCGTGCCCATGGT
				CTGGACAAGGACTTCAAGGTCAACATCGAGGTGAACCACGCTACACTGGCCGGACACACC
				TTCGAGCGCGAACTGGCAGTGGCCGTCGACAACGGTCTACTCGGCTCAATCGACGCCAAC
				CGTGGTGACTATCAGAATGGTTGGGACACCGATCAGTTCCCCATCGACCACTATGAGTTG
				GTTCAGGGCATGTTGCAGATTATCCGCAATGGTGGTTTCACCGACGGTGGCACCAACTTC
				GATGCCAAGACCCGCCGCAACTCGACCGACCTCGAGGACATCTTCATCGCCCACATCGCC
				GCGATGGATGCCATGGCTCATGCGCTGGAGAGTGCTGCCTCCATCATCGAGGAGTCGCCC
				TACTGCCAGATGGTCAAGGATCGCTATGCCTCATTTGACTCCGGCATCGGCAAGGACTTT
				GAGGACGGCAAGTTGACACTGGAACAAGCCTACGAGTACGGTAAGCAAGTGGGCGAACCC
				AAGCAGACCAGTGGCAAGCAAGAACTGTACGAGTCAATCATCAATATGTATTCCATTTAA
5610MI3_003	Bacteroides	Amino	22	MATKEFFPEIGKIKFEGRESRNPLAFRYYGPEKVVLGKKMKDWFKFAMAWWHTLCAQGTD
		Acid		QFGGDTKQFPWNTASDPMQAAKDKVDAGFEFMTKMGIEYFCFHDVDLVAEAATVEEYEAN
				LKTIVAYIKEKQAETGIKNLWGTANVFGHKRYMNGAATNPDFDVVARAIVQIKNAIDATI
				ELGGTSYVFWGGREGHMSLLNTDQKREKEHLARMLTMARDYARARGFNGTFLIEPKPMEP
				TKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFERELAVAVDNGLLGSIDAN
				RGDYQNGWDTDQFPIDHYELVQGMLQIIRNGGFTDGGTNFDAKTRRNSTDLEDIFIAHIA
				AMDAMAHALESAASIIEESPYCQMVKDRYASFDSGIGKDFEDGKLTLEQAYEYGKQVGEP
				KQTSGKQELYESIINMYSI
5749MI2_004	Bacteroides	DNA	23	ATGGCAACAAAAGAGTATTTTCCTGGTATAGGAAAGATTAAATTTGAAGGTAAAGAGAGT
				AAGAATCCGATGGCATTCCGCTATTATGATGCCAATAAAGTAATCATGGGCAAGAAGATG
				AGCGAGTGGCTGAAGTTTGCCATGGCTTGGTGGCACACATTGTGCGCCGAAGGTGGTGAC
				CAGTTTGGTGGTGGAACAAAGACTTTCCCGTGGAACGATTCGGACAACGCCGTAGAAGCA
				GCCAACCATAAAGTAGATGCCGGTTTTGAATTTATGCAGAAAATGGGCATCGAATACTAT
				TGCTTCCATGATGTAGACCTCTGCACTGAAGCTGCTACCATTGAAGAATATGAAGCCAAT
				CTGAAGGAAATAGTAGCCTATCCGAAACAGAAACAGGCTGAAACAGGTATCAAACTTCTG
				TGGGGTACGGCAAATGTATTTGGTCACAAACGCTATATGAATGGTGCTGCTACCAATCCG
				GATTTTGATGTAGTGGCTCGTGCTGCTGTACAGATTAAGAATGCGATAGACGCTACAATT
				GAACTCGGTGGTAGCAACTACGTGTTCTGGGGCGGCCGTGAAGGTTATATGAGCTTGCTC
				AATACAGACCAGAAACGTGAGAAAGAGCATTTGGCACAAATGTTGACCATGGCTCGTGAC
				TATGCTCGTGCCAAAGGATTCAAGGGTACCTTCCTGGTTGAACCCAAACCGATGGAACCA
				ACTAAACACCAGTATGATGTAGATACGGAAACTGTAATCGGCTTCCTCAAGGCTCATAAT
				TTGGATAAGGATTTCAAGGTAAATATTGAAGTAAACCATGCTACATTGGCCGGTCATACT
				TTTGAACACGAATTGGCTGTTGCCGTAGACAACGATATGCTTGGCTCTATCGATGCCAAC
				CGCGGTGACTATCAGAACGGTTGGGATACTGACCAGTTCCCCATTGACAACTTCGAGCTT
				ATCCAAGCCATGATGCAGATTATTCGCGGTGGTGGCTTCAAAGATGGTGGTACAAACTTC
				GACGCTAAGACTCGTCGTAACTCTACCGACCTGGAAGATATTTTCATTGCACACATCGCT
				GGTATGGATGCTATGGCACGTGCTTTGGAAAGTGCAGCCAAGTTGCTTGAGGAATCTCCT
				TATAAGAAAATGTTGGCTGACCGCTATGCATCGTTCGATAGTGGCAAAGGTAAGGAGTTT
				GAAGAAGGCAAGCTGACATTGGAAGACGTTGTAGTTTATGCCAAGCAGAATGGCGAGCCT
				AAACAGACCAGCGGTAAGCAGGAATTGTATGAGGCAATTGTAAATATGTATGCCTGA
5749MI2_004	Bacteroides	Amino	24	MATKEYFPGIGKIKFEGKESKNPMAFRYYDANKVIMGKKMSEWLKFAMAWWHTLCAEGGD
		Acid		QFGGGTKTFPWNDSDNAVEAANHKVDAGFEFMQKMGIEYYCFHDVDLCTEAATIEEYEAN
				LKEIVAYPKQKQAETGIKLLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNAIDATI
				ELGGSNYVFWGGREGYMSLLNTDQKREKEHLAQMLTMARDYARAKGFKGTFLVEPKPMEP
				TKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNDMLGSIDAN
				RGDYQNGWDTDQFPIDNFELIQAMMQIIRGGGFKDGGTNFDAKTRRNSTDLEDIFIAHIA
				GMDAMARALESAAKLLEESPYKKMLADRYASFDSGKGKEFEEGKLTLEDVVVYAKQNGEP
				KQTSGKQELYEAIVNMYA
5750MI3_003	Bacteroides	DNA	25	ATGGCAACAAAAGAGTATTTTCCTGGAATAGGAAAGATTAAATTTGAAGGAAAAGAGAGT
				AAGAACCCGATGGCATTCCGTTGCTACGATGCAGAAAAAGTTATCATGGGTAAGAGAATG
				AAAGATTGGTTGAAGTTTGCAATGGCGTGGTGGCATACACTTTGTGCAGAAGGCGGTGAC
				CAATTCGGTGGCGGTACAAAGAGTTTCCCCCGGAACGACTATACTGATAAAATTCAGGCT
				GCTAAAAACAAGATGGATGCCGGTTTTGAGTTTATGCAGAAGATGGGGATCGAATACTAT
				TGTTTTCACGATGTAGACCTCTGCACGGAAGCTGATACCATTGAAGAATACGAAGCTAAT
				TTGAAAGAAATCGTAGTTTACGCAAAGCAAAAGCAGGTAGAAACAGGTATCAAATTATTG
				TGGGGTACTGCCAATGTATTCGGTCATGAACGCTATATGAATGGTGCGGCTACCAACCCA
				GATTTTGATGTTGTAGCCCGTGCTGCTGTTCAGATTAAGAATGCAATTGATGCTACCATT
				GAACTAGGTGGCTTAAACTATGTGTTCTGGGGTGGACGCGAAGGTTATATGTCTTTGCTG
				AACACTGATCAGAAACGTGAGAAAGAACATCTTGCACAAATGCTGACCATTGCCCGTGAC
				TATGCCCGTGCCCGTGGCTTCAAAGGTACATTCTTGGTTGAACCGAAACCGATGGAACCA
				ACCAAACATCAATATGACGTAGATACAGAAACAGTTATCGGTTTTTTGAAAGCTCATGCT
				TTGGATAAAGACTTTAAAGTAAATATTGAAGTAAATCATGCAACATTAGCCGGTCATACA
				TTTGAACACGAACTGGCAGTGGCTGTCGACAACGGTATGCTGGGTTCTATTGACGCTAAT
				CGTGGTGATTGTCAAAACGGTTGGGATACAGACCAATTTCCCATTGATAACTATGAACTG
				ACTCAAGCCATGATGCAGATTATTCGTAACGGTGGTTTGGGCAATGGTGGTACGAATTTT
				GACGCTAAAACTCGCCGTAATTCTACTGATCTTGGAGATATCTTCATTGCTCACATCGCA
				GGTATGGATGCTATGGCACGTGCATTGGAAAGTGCGGCCAAGTTGTTGGAAGAATCTCCC
				TATAAGAAGATGCTGGCAGAACGTTATGCATCCTTTGACAGCGGTAAGGGTAAAGAGTTT
				GAAGAGGGTAAGTTGACCTTGGAGGATCTTGTTGCTTATGCAAAAGTCAATGGCGAACCG
				AAACAAATCAGTGGTAAACAAGAATTGTATGAGGCAATTGTGAATATGTATTGCTAA
5750MI3_003	Bacteroides	Amino	26	MATKEYFPGIGKIKFEGKESKNPMAFRCYDAEKVIMGKRMKDWLKFAMAWWHTLCAEGGD
		Acid		QFGGGTKSFPRNDYTDKIQAAKNKMDAGFEFMQKMGIEYYCFHDVDLCTEADTIEEYEAN
				LKEIVVYAKQKQVETGIKLLWGTANVEGHERYMNGAATNPDFDVVARAAVQIKNAIDATI
				ELGGLNYVFWGGREGYMSLLNTDQKREKEHLAQMLTIARDYARARGFKGTFLVEPKPMEP
				TKHQYDVDTETVIGFLKAHALDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDAN
				RGDCQNGWDTDQFPIDNYELTQAMMQIIRNGGLGNGGTNFDAKTRRNSTDLGDIFIAHIA
				GMDAMARALESAAKLLEESPYKKMLAERYASFDSGKGKEFEEGKLTLEDLVAYAKVNGEP
				KQISGKQELYEAIVNMYC
5750MI4_003	Bacteroides	DNA	27	ATGGCAACAAAAGAGTATTTTCCCGGAATAGGAAAGATTAAATTCGAAGGTAAAGAGAGC
				AAGAACCCGATGGCATTCCGTTATTACGATGCCGATAAAGTAATCATGGGTAAGAAAATG
				AGCGAATGGCTGAAGTTCGCCATGGCATGGTGGCACACTCTTTGCGCAGAAGGTGGTGAC
				CAGTTCGGTGGCGGAACAAAGAAATTCCCCTGGAACGGTGAGGCTGACAAGGTTCAGGCT
				GCCAAGAACAAAATGGACGCCGGCTTTGAATTCATGCAGAAAATGGGTATCGAATACTAC
				TGCTTCCACGATGTAGACCTCTGCGAAGAAGCCGAGACCATTGAAGAATACGAAGCCAAC
				TTGAAGGAAATCGTAGCGTATGCCAAGCAGAAACAAGCAGAAACCGGCATCAAGCTGTTG
				TGGGGTACTGCCAACGTATTCGGCCATGCCCGCTACATGAATGGTGCAGCCACCAACCCC
				GATTTCGATGTTGTGGCACGTGCAGCCGTCCAAATCAAAAGCGCCATCGACGCTACTATC
				GAGCTGGGAGGTTCGAACTATGTGTTCTGGGGCGGTCGCGAAGGCTACATGTCATTGCTG
				AATACAGACCAGAAGCGTGAGAAAGAGCACCTCGCACAGATGTTGACCATCGCCCGCGAC
				TATGCCCGTGCCCGTGGCTTCAAAGGTACCTTCCTGATTGAACCGAAACCGATGGAACCT
				ACAAAACACCAGTATGATGTAGACACCGAAACCGTTATCGGCTTCTTGAAGGCCCACAAT
				CTGGACAAAGATTTCAAGGTAAACATCGAAGTGAACCACGCTACTTTGGCGGGCCACACC
				TTCGAGCACGAACTCGCAGTAGCCGTAGACAACGGTATGCTCGGCTCCATCGATGCCAAC
				CGTGGTGACTACCAGAACGGCTGGGATACAGACCAGTTCCCCATTGACAACTTCGAACTG
				ACCCAGGCAATGATGCAAATCATCCGTAACGGCGGCTTTGGCAATGGCGGTACAAACTTC
				GATGCCAAGACCCGTCGTAACTCCACCGACCTGGAAGACATCTTGATTGCCCACATCGCC
				GGTATGGACGTGATGGCACGTGCACTGGAAAGTGCAGCCAAATTGCTTGAAGAGTCTCCT
				TACAAGAAGATGCTTGCCGACCGCTATGCTTCCTTCGACAGTGGTAAAGGCAAGGAATTC
				GAAGACGGCAAGCTGACACTGGAGGATTTGGCAGCTTACGCAAAAGCCAACGGTGAGCCG
				AAACAGACCAGCGGCAAGCAGGGATTGTATGAGGCAATCGTAAATATGTACTGCTGA
5750MI4_003	Bacteroides	Amino	28	MATKEYFPGIGKIKFEGKESKNPMAFRYYDADKVIMGKKMSEWLKFAMAWWHTLCAEGGD
		Acid		QFGGGTKKFPWNGEADKVQAAKNKMDAGFEFMQKMGIEYYCFHDVDLCEEAETIEEYEAN
				LKEIVAYAKQKQAETGIKLLWGTANVFGHARYMNGAATNPDFDVVARAAVQIKSAIDATI
				ELGGSNYVFWGGREGYMSLLNTDQKREKEHLAQMLTIARDYARARGFKGTFLIEPKPMEP
				TKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDAN
				RGDYQNGWDTDQFPIDNFELTQAMMQIIRNGGFGNGGTNFDAKTRRNSTDLEDIFIAHIA
				GMDVMARALESAAKLLEESPYKKMLADRYASFDSGKGKEFEDGKLTLEDLAAYAKANGEP
				KQTSGKQGLYEAIVNMYC
5751MI4_002	Bacteroides	DNA	29	ATGACAAAAGAGTATTTTCCAACCATTGGTAAAATTCAGTTTGAAGGTAAAGAGAGTAAG
				AATCCATTAGCATATCGTTATTACGATGCTAACAAAGTAATAATGGGTAAAAAGATGAGC
				GAATGGCTCAAGTTTGCAATGGCATGGTGGCACACTTTGTGTGCTGAGGGTAGCGACCAG
				TTTGGTCCTGGCACCAAGTCATTCCCATGGAACGCATCAACCGACCGTATGCAGGCTGCA
				AAAGATAAGGCTGACGCAGGCTTCGAAATCATGCAAAAACTGGGCATCGAATACTACTGT
				TTCCATGATGTTGACCTCATCGACCCAGCAGACGATATTCCAACATACGAAAAGAATCTC
				AAGGAAATCGTTGCATACCTCAAGCAAAAACAGGCCGAGACAGGTATCAAATTGCTATGG
				GGTACAGCTAACGTATTTGGCCACAAGCGTTATATGAACGGTGCATCTACCAATCCTGAC
				TTTGACGTTGTTGCACGAGCTATCGTGCAAATCAAGAATGCTATCGATGCAACAATCGAA
				CTGGGCGGCACGAACTACGTATTCTGGGGTGGTCGCGAAGGTTACATGTCACTGCTCAAC
				ACCGACCAAAAGCGCGAGAAAGAGCACATGGCTACCATGTTAGGAATGGCACGTGACTAT
				GCACGTTCTAAAGGCTTTACTGGTACTCTCCTTATCGAGCCAAAGCCTATGGAACCAACT
				AAGCATCAATACGACGTCGATACAGAAACTGTTATTGGTTTCCTCAAAGCTCACGGATTA
				GACAAGGACTTCAAGGTAAATATCGAAGTGAACCACGCTACATTGGCTGGCCATACCTTC
				GAACATGAATTAGCATGTGCTGTTGATGCAGGTATGCTTGGTTCCATCGATGCTAACCGT
				GGTGATATGCAGAATGGCTGGGATACAGATCAGTTCCCTATCAACAATTACGAGCTCGTT
				CAGGCCATGATGCAGATTATCCGCAATGGTGGTTTCGGTAACGGTGGTACAAACTTCGAC
				GCTAAGACACGTCGTAATTCAACCGATTTGGAAGACATCATCATTGCTCACGTTTCAGCT
				ATGGATGCTATGGCACGTGCTCTTGAATGTGCTGCAGACATTCTTCAAAACTCACCTATT
				CCACAGATGGTGGCCAACCGTTATGCAAGTTTTGACAAGGGTATAGGTAAAGATTTCGAA
				GACGGCAAGCTCACCCTCGAGCAAGTATACGAATATGGTAAGACCGTCGGCGAACCAGCT
				ATTACAAGCGGCAAACAGGAGCTCTACGAAGCTATCGTTAATATGTATTGCTGA
5751MI4_002	Bacteroides	Amino	30	MTKEYFPTIGKIQFEGKESKNPLAYRYYDANKVIMGKKMSEWLKFAMAWWHTLCAEGSDQ
		Acid		FGPGTKSFPWNASTDRMQAAKDKADAGFEIMQKLGIEYYCFHDVDLIDPADDIPTYEKNL
				KEIVAYLKQKQAETGIKLLWGTANVFGHKRYMNGASTNPDFDVVARAIVQIKNAIDATIE
				LGGTNYVFWGGREGYMSLLNTDQKREKEHMATMLGMARDYARSKGFTGTLLIEPKPMEPT
				KHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANR
				GDMQNGWDTDQFPINNYELVQAMMQIIRNGGFGNGGTNFDAKTRRNSTDLEDIIIAHVSA
				MDAMARALECAADILQNSPIPQMVANRYASFDKGIGKDFEDGKLTLEQVYEYGKTVGEPA
				ITSGKQELYEAIVNMYC
5751MI5_003	Bacteroides	DNA	31	ATGGCTAACAAAGAATTTTTCCCCGGTATTGGTAAAATCAAATTCGAAGGTAAAGAGAGC
				AAGAACCCCATGGCATATCGTTACTACGATGCTGAGAAGGTAGTCCTTGGCAAGAATATG
				AAAGACTGGTTCAAGTTTGCGATGGCTTGGTGGCACACATTGTGCGCCGAGGGTAGCGAC
				CAGTTTGGTCCCGGCACTAAGTCTTTCCCCTGGAACACCGCAGAGTGCCCCATGCAGGCA
				GCTAAGGACAAGGTTGACGCTGGCTTCGAGTTCATGACCAAGATGGGTATTGAATACTTC
				TGCTTCCACGATGTAGACCTCGTTGCCGAGGCCGACACTGTTGAGGAGTACGAGGCTCGC
				ATGAAGGAAATCGTTGCTTACATCAAGGAGAAGGTGGCCGAGACTGGCATCAAGAACCTG
				TGGGGTACAGCTAACGTATTTGGCAACAAGCGCTACATGAACGGTGCTGCTACTAACCCC
				GACTTTGACGTTGTGGCTCGCGCTATCGTTCAAATCAAGAACGCTATCGACGCTACTATC
				GAGCTCGGTGGTACGTCATACGTATTCTGGGGCGGCCGCGAGGGTTACATGAGCCTCTTG
				AACACCGACCAGAAGCGTGAGAAAGAGCACCTGGCTACTATGCTCACTATGGCACGCGAC
				TACGCTCGCGCTAAGGGTTTCAAGGGTACATTCCTCATCGAGCCCAAGCCCATGGAGCCC
				ACAAAGCACCAGTACGATGTTGACACTGAGACTGTAATCGGCTTCCTTAAGGCACACAAC
				CTTGACAAGGACTTCAAGGTTAACATTGAGGTTAACCACGCAACTCTCGCTGGTCACACA
				TTTGAGCACGAGCTCGCTTGTGCTGTTGACGCTGGCATGCTTGGCAGCATCGACGCTAAC
				CGCGGTGACTACCAGAACGGCTGGGATACTGACCAATTCCCCATCGACAACTTCGACCTC
				ACTCAAGCTATGCTCGAGATCATCCGCAACGATGGTTTCAAGGATGGTGGTACAAACTTC
				GACGCTAAGACTCGCCGCAACAGCACCGACCTCGAGGATATCTTCATCGCACACATCGCT
				GCTATGGACGCTATGGCACGTGCTCTCGAGAGCGCTGCTGCAGTACTCGAGGAGTCAGCT
				CTGCCCCAAATGAAGAAGGACCGCTATGCATCGTTCGACGCTGGCATGGGTAAGGACTTC
				GAGGACGGCAAGCTCACCCTGGAGCAAGTTTACGAGTATGGTAAGAAGGTGGGCGAGCCC
				AAGCAGACTAGCGGCAAGCAAGAGCTGTATGAGGCTATCCTCAACATGTACGTATAA
5751MI5_003	Bacteroides	Amino	32	MANKEFFPGIGKIKFEGKESKNPMAYRYYDAEKVVLGKNMKDWFKFAMAWWHTLCAEGSD
		Acid		QFGPGTKSFPWNTAECPMQAAKDKVDAGFEFMTKMGIEYFCFHDVDLVAEADTVEEYEAR
				MKEIVAYIKEKVAETGIKNLWGTANVFGNKRYMNGAATNPDFDVVARAIVQIKNAIDATI
				ELGGTSYVFWGGREGYMSLLNTDQKREKEHLATMLTMARDYARAKGFKGTFLIEPKPMEP
				TKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDYQNGWDTDQFPIDNFDLTQAMLEIIRNDGFKDGGTNFDAKTRRNSTDLEDIFIAHIA
				AMDAMARALESAAAVLEESALPQMKKDRYASFDAGMGKDFEDGKLTLEQVYEYGKKVGEP
				KQTSGKQELYEAILNMYV
5751MI6_004	Bacteroides	DNA	33	ATGGCTAACAAAGAATTTTTCCCAGGTATTGGTAAAATCAAATTCGAAGGCAAAGAAAGC
				AAGAACCCCATGGCATATCGTCACTACGATGCCGAGAAGGTAGTCCTTGGTAAGAAGATG
				AAGGACTGGTTCAAGTTTGCGATGGCTTGGTGGCACACTCTGTGCGCCGAGGGTAGCGAC
				CAGTTCGGCCCCGTGACCAAGTCTTTCCCCTGGAACCAGGCCGAGTGCCCCATGCAGGCT
				GCTAAGGACAAGGTTGACGCCGGCTTCGAGTTCATGACCAAGATGGGTATCGAATACTTC
				TGTTTCCACGATGTAGACCTCGTTGCCGAGGCCGACACCGTTGAGGAGTACGAAGCTCGC
				ATGAAGGAAATCGTGGCTTACATCAAGGAGAAGATGGCCGAGACCGGCATCAAGAACCTG
				TGGGGTACAGCCAACGTATTCGGCAACAAGCGCTACATGAACGGTGCTGCCACCAACCCC
				GACTTTGACGTTGTGGCTCGCGCAATCGTTCAGATCAAGAACGCCATCGACGCTACTATC
				GAGCTCGGCGGTACCTCTTACGTGTTCTGGGGCGGCCGCGAGGGTTACATGACTCTCTTG
				AACACCGACCAGAAGCGCGAGAAGGAGCACCTGGCTACCATGCTCACCATGGCTCGCGAC
				TATGCTCGCGCTAAGGGCTTCAAGGGTACATTCCTTATCGAGCCCAAGCCCATGGAGCCC
				ACCAAGCACCAGTATGACGTGGATACCGAGACCGTTATCGGCTTCCTCAAGGCTCACGGC
				CTGGACAAGGACTTCAAGGTGAACATCGAGGTTAACCATGCAACTCTCGCCGGCCACACA
				TTCGAGCACGAACTCGCTTGCGCTGTTGACGCTGGCATGCTGGGCAGCATCGACGCTAAC
				CGCGGCGACTACCAGAACGGCTGGGATACCGACCAGTTCCCCATCGACAACTTCGACCTC
				ACTCAGGCTATGCTCGAGATCATCCGCAACGGTGGTTTCAAGGACGGTGGTACAAACTTC
				GACGCTAAGACCCGTCGCAACAGCACCGATCTTGAGGACATCTTCATCGCTCACATCGCT
				GCTATGGACGCAATGGCACGCGCGCTCGAGAGCGCTGCCGCTGTGCTCGAGCAGAGCCCC
				CTTCCCCAGATGAAGAAAGACCGCTACGCATCGTTCGATGCCGGCATGGGCAAGGACTTC
				GAGGACGGCAAGCTCACTCTGGAGCAGGTTTACGAGTATGGTAAGAAGGTAGGCGAGCCC
				AAGCAGACCAGCGGCAAGCAGGAACTGTACGAGGCTATCCTCAACATGTATGTATAA
5751MI6_004	Bacteroides	Amino	34	MANKEFFPGIGKIKFEGKESKNPMAYRHYDAEKVVLGKKMKDWFKFAMAWWHTLCAEGSD
		Acid		QFGPVTKSFPWNQAECPMQAAKDKVDAGFEFMTKMGIEYFCFHDVDLVAEADTVEEYEAR
				MKEIVAYIKEKMAETGIKNLWGTANVFGNKRYMNGAATNPDFDVVARAIVQIKNAIDATI
				ELGGTSYVFWGGREGYMTLLNTDQKREKEHLATMLTMARDYARAKGFKGTFLIEPKPMEP
				TKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDYQNGWDTDQFPIDNFDLTQAMLEIIRNGGFKDGGTNFDAKTRRNSTDLEDIFIAHIA
				AMDAMARALESAAAVLEQSPLPQMKKDRYASFDAGMGKDFEDGKLTLEQVYEYGKKVGEP
				KQTSGKQELYEAILNMYV
5586MI22_003	Clostridiales	DNA	35	ATGAAAGAATATTTTCCTATGACAAAAAAAGTTGAATATGAGGGCGCAGCATCTAAAAAT
				CCATTTGCGTTTAAATACTATGATGCCGAAAGAATTATAGCAGGCAAGCCTATGAAAGAA
				CATCTTAAATTTGCTATGAGTTGGTGGCATACACTTTGTGCGGGCGGTGCAGACCCATTT
				GGCACAACAACTATGGACAGAACATACGGCGGACTTACCGACCCAATGGAAATTGCAAAG
				GCAAAAGTAGATGCAGGCTTTGAGTTTATGCAAAAACTCGGTATAGAGTATTTTTGTTTT
				CACGATGCGGATATTGCACCGGAAGGAAGCAGTTTTGTTGAAACAAAGAAAAACTTTTGG
				GAAATAGTAGATTATATACAGCAAAAGATGAATGAAACAGGCATAAAGTTGCTTTGGGGT
				ACTGCAAACTGCTTTAATGCTCCACGTTATATGCACGGTGCAGGAACATCATGCAATGCG
				CACAGTTTTGCATATGCAGCCGCACAGATAAAAAATGCAATTGAAGCTACCGTTAAACTG
				GGTGGAAAAGGCTATGTTTTCTGGGGCGGAAGAGAGGGTTATGAAACACTTCTCAATACG
				GATATGGCACTTGAACTTGACAATATGGCAAGACTTATGCATATGGCAGTTGATTATGGC
				AGAAGCATTGGTTTTGACGGTGATTTTTATATCGAACCAAAGCCAAAGGAACCAACAAAA
				CATCAATATGACTTTGACTCGGCAACTGTTTTGGGATTTTTGAGAAAGTACGGTTTAGAT
				AAGGATTTTAAACTTAATATAGAGGCAAATCATGCGACACTTGCAGGTCATACATTTGAA
				CATGAATTGACTGTAGCGCGTATAAACGGTGCATTTGGCAGCATAGATGCAAATAGCGGC
				GATCCCAATCTTGGCTGGGATACCGACCAATTCCCAACAGATGTTTATTCGGCAACCCTT
				TGTATGCTTGAAGTGATAAGAGCAGGCGGCTTTACAAACGGAGGTCTTAATTTTGATGCA
				AAGGTCAGAAGAGGCTCATTTACGTTTGATGACATTGTTTATGCATATATCAGCGGTATG
				GACACTTTTGCGCTGGGTTTTATAAAGGCATATGAAATAATTGAGGACGGCAGAATAGAT
				GAATTTGTAAAAGAAAGATACGCAAGCTATAATACAGGCATAGGCAAAGATATTATAGAT
				GGAAAGGCAAGCCTTGAAAGTTTGGAAGAATATATTCTTTCAAATGATAATGTTGTAATG
				CAAAGCGGCAGACAGGAATATCTTGAAACAGTTTTGAATAATATTTTGTTTAAAGCATAA
5586MI22_003	Clostridiales	Amino	36	MKEYFPMTKKVEYEGAASKNPFAFKYYDAERIIAGKPMKEHLKFAMSWWHTLCAGGADPF
		Acid		GTTTMDRTYGGLTDPMEIAKAKVDAGFEFMQKLGIEYFCFHDADIAPEGSSFVETKKNFW
				EIVDYIQQKMNETGIKLLWGTANCFNAPRYMHGAGTSCNAHSFAYAAAQIKNAIEATVKL
				GGKGYVFWGGREGYETLLNTDMALELDNMARLMHMAVDYGRSIGEDGDFYIEPKPKEPTK
				HQYDFDSATVLGELRKYGLDKDFKLNIEANHATLAGHTFEHELTVARINGAFGSIDANSG
				DPNLGWDTDQFPTDVYSATLCMLEVIRAGGFTNGGLNFDAKVRRGSFTFDDIVYAYISGM
				DTFALGFIKAYEIIEDGRIDEFVKERYASYNTGIGKDIIDGKASLESLEEYILSNDNVVM
				QSGRQEYLETVLNNILFKA
1753MI4_001	Firmicutes	DNA	37	ATGAAAGAAATTTTCCCAAATATTCCTGAGATTAAATTCGAAGGAAAAGACAGCAAAAAT
				CCTTTTGCTTTCCATTACTACAACCCAGACCAAATCATCTTAGGCAAACCAATGAAAGAA
				CACCTCCCATTCGCTATGGCTTGGTGGCACAATCTTGGTGCAACAGGTGTTGATATGTTT
				GGCGCTGGCCCAGCTGATAAGAGTTTCGGTGCTAAAGTTGGCACAATGGAACACGCTAAG
				GCCAAAGTCGATGCCGGTTTCGAATTCATGAAGAAACTCGGTATCAGATATTTCTGCTTC
				CATGATGTTGACTTAGTTCCAGAATGTGCAGATATCAAAGATACAAACAAAGAATTAGAT
				GAAATCAGTGACTACATCTTAGAAAAGATGAAAGGCACAGATATTAAGTGTTTATGGGGC
				ACCGCCAATATGTTCTCTAACCCACGCTTCTGCAATGGTGCGGGTTCCACAAACAGTGCG
				GATGTCTTCGCTTTCGCCGCTGCTCAAGTTAAGAAAGCCTTAGATATCACCGTTAAATTA
				GGTGGTAGGGGTTACGTCTTCTGGGGTGGTCGTGAAGGTTACGAAACATTACTCAATACA
				GACGTTAAATTCGAACAAGAAAACATTGCTCGTTTAATGAAGATGGCTGTTGAATATGGC
				CGTTCCATCGGTTTCAAAGGCGATTTCTATATCGAACCAAAACCAAAAGAACCAATGAAA
				CACCAATATGACTTCGACGCCGCTACAGCTATTGGCTTCTTAAGAGCCCACGGCTTAGAC
				AAAGACTTCAAGTTGAACATCGAAGCTAACCACGCTACATTAGCGGGTCATACATTCCAA
				CACGATTTAAGAATCTCCGCCATTAATGGTATGTTAGGTTCTATCGATGCTAACCAAGGC
				GATATGCTCTTAGGTTGGGATACAGACGAATTCCCATTTGATGTCTACAGTGCGACACAA
				TGTATGTACGAAGTCTTAAAGAATGGTGGTCTTACAGGTGGTTTCAACTTTGACTCCAAA
				ACACGTCGTCCATCCTACACAATGGAAGATATGTTCTTAGCCTATATCTTAGGTATGGAT
				ACATTCGCTTTAGGTTTAATCAAAGCTGCTCAAATCATCGAAGATGGCCGTATTGATCAA
				TTCATCGAAAAGAAATATTCTTCCTTCCGTGAAACAGAAATCGGTCAAAAGATCTTAAAC
				AACAAGACAAGCTTAAAAGAATTATCCGATTACGCTTGCAAGATGGGTGCTCCAGAACTT
				CCAGGTAGTGGTCGTCAAGAAATGCTCGAAGCCATCGTTAACGATGTCTTATTCGGCAAG
				TAA
1753MI4_001	Firmicutes	Amino	38	MKEIFPNIPEIKFEGKDSKNPFAFHYYNPDQIILGKPMKEHLPFAMAWWHNLGATGVDMF
		Acid		GAGPADKSFGAKVGTMEHAKAKVDAGFEFMKKLGIRYFCFHDVDLVPECADIKDTNKELD
				ETSDYILEKMKGTDIKCLWGTANMESNPRECNGAGSTNSADVFAFAAAQVKKALDITVKL
				GGRGYVFWGGREGYETLLNTDVKFEQENIARLMKMAVEYGRSIGFKGDFYIEPKPKEPMK
				HQYDFDAATAIGFLRAHGLDKDFKLNIEANHATLAGHTFQHDLRISAINGMLGSIDANQG
				DMLLGWDTDEFPFDVYSATQCMYEVLKNGGLTGGFNFDSKTRRPSYTMEDMFLAYILGMD
				TFALGLIKAAQIIEDGRIDQFIEKKYSSFRETEIGQKILNNKTSLKELSDYACKMGAPEL
				PGSGRQEMLEAIVNDVLFGK
1753MI6_001	Firmicutes	DNA	39	ATGAAAGAAATTTTCCCAAATATTCCTGAGATTAAATTCGAAGGAAAAGACAGCAAAAAT
				CCTTTTGCTTTCCATTACTACAACCCAGACCAAATCATCTTAGGTAAACCAATGAAAGAA
				CACCTCCCATTCGCTATGGCTTGGTGGCACAATCTTGGTGCAACAGGTGTTGATATGTTT
				GGCGCTGGCCCAGCTGATAAGAGTTTCGGTGCTAAAGTTGGCACAATGGAACACGCTAAG
				GCCAAAGTCGATGCCGGTTTCGAATTCATGAAGAAACTTGGTATCAGATATTTCTGCTTC
				CATGATGTTGACTTAGTTCCAGAATGTGCAGATATCAAAGATACAAACAAAGAATTAGAT
				GAAATCAGTGACTACATCTTAGAAAAGATGAAAGGCACAGATATCAAGTGTTTATGGGGC
				ACCGCCAATATGTTCTCTAACCCACGTTTCTGCAATGGTGCGGGTTCCACAAACAGTGCG
				GATGTCTTCGCTTTCGCCGCTGCTCAAGTTAAGAAAGCCTTAGATATCACCGTTAAATTA
				GGTGGTAGGGGTTACGTCTTCTGGGGTGGTCGTGAAGGTTACGAAACATTACTCAATACA
				GACGTTAAATTCGAACAAGAAAACATTGCTCGTTTAATGAAGATGGCTGTTGAATATGGC
				CGTTCCATCGGTTTCAAAGGCGATTTCTATATCGAACCAAAACCAAAAGAACCAATGAAA
				CACCAATATGACTTCGACGCCGCTACAGCTATTGGCTTCTTAAGAGCCCACGGCTTAGAC
				AAAGACTTCAAGTTGAACATCGAAGCTAACCACGCTACATTAGCGGGTCATACATTCCAA
				CACGATTTAAGAATCTCCGCCATTAATGGTATGTTAGGTTCTATCGATGCTAACCAAGGC
				GATATGCTCTTAGGTTGGGATACAGACGAATTCCCATTTGATGTCTACAGTGCGACACAA
				TGTATGTACGAAGTCTTAAAGAATGGTGGTCTTACAGGTGGTTTCAACTTTGACTCCAAA
				ACACGTCGTCCATCCTACACAATGGAAGATATGTTCTTAGCCTATATCTTAGGTATGGAT
				ACATTCGCTTTAGGTTTAATCAAAGCTGCTCAAATCATCGAAGATGGCCGTATTGATCAA
				TTCATCGAAAAGAAATATTCTTCCTTCCGTGAAACAGAAATCGGTCAAAAGATCTTAAAC
				AACAAGACAAGCTTAAAAGAATTATCCGATTACGCTTGCAAGATGGGTGCTCCAGAACTT
				CCAGGTAGTGGTCGTCAAGAAATGCTCGAAGCCATCGTTAACGATGTCTTATTCGGCAAG
				TAA
1753MI6_001	Firmicutes	Amino	40	MKEIFPNIPEIKFEGKDSKNPFAFHYYNPDQIILGKPMKEHLPFAMAWWHNLGATGVDMF
		Acid		GAGPADKSFGAKVGTMEHAKAKVDAGFEFMKKLGIRYFCFHDVDLVPECADIKDTNKELD
				EISDYILEKMKGTDIKCLWGTANMFSNPRFCNGAGSTNSADVFAFAAAQVKKALDITVKL
				GGRGYVFWGGREGYETLLNTDVKFEQENIARLMKMAVEYGRSIGFKGDFYIEPKPKEPMK
				HQYDFDAATAIGFLRAHGLDKDFKLNIEANHATLAGHTFQHDLRISAINGMLGSIDANQG
				DMLLGWDTDEFPFDVYSATQCMYEVLKNGGLTGGFNFDSKTRRPSYTMEDMFLAYILGMD
				TFALGLIKAAQIIEDGRIDQFIEKKYSSFRETEIGQKILNNKTSLKELSDYACKMGAPEL
				PGSGRQEMLEAIVNDVLFGK
1753MI35_004	Firmicutes	DNA	41	ATGGAATATTTCCCTTTCGTCAAATCGGTCCAATACAAGGGACCAACCTCAACTGAACCA
				TTCGCTTTCAAGTACTACGATGCCAACCGTGTCGTTCTTGGAAAACCAATGAAAGAATGG
				ATGCCATTCGCTATGGCTTGGTGGCACAACCTCGGCGCTGCCGGTACCGACATGTTCGGC
				GGCAACACCATGGACAAGTCCTGGGGAGTCGATAAAGAAAAAGACCCAATGGGCTATGCC
				AAAGCCAAAGTTGATGCCGGCTTCGAATTCATGCAGAAGATGGGCATCGAATACTACTGC
				TTCCACGATGTCGACCTCGTCCCAGAGTGCGACGACATCACCGTTATGTACCAGAGACTC
				GATGAGATCGGTGATTACCTTCTCAAGAAACAGAAGGAAACCGGTATCAAGCTTCTTTGG
				TCAACCGCCAATGCCTTCGGACACCGCCGTTTCATGAACGGTGCTGGTTCCAGCAACTCC
				GCCGAAGTCTATTGCTTCGCCGCCGCCCAGATCAAGAAAGCTCTTGAGCTCTGCGTCAAA
				CTCGGTGGCAAAGGCTATGTCTTCTGGGGTGGACGTGAAGGCTACGAAACCCTTCTCAAC
				ACCGACATGAAGTTCGAACAAGAGAACATCGCCAACCTTATGAGATGCGCCCGTGACTAC
				GGCCGCAAGATCGGTTTCAAAGGCGACTTCTACATCGAACCAAAACCAAAAGAGCCAACA
				AAGCATCAGTATGACTTCGACGCCGCTACCGCCATCGGATTCCTCCGTCAGTACGGTCTC
				GACAAAGACTTCAAGATGAACATCGAAGCCAACCACGCTACCTTAGCTGGCCACACCTTC
				GAACACGAACTCCGCGTCTCCGCCATGAACGGCATGCTCGGTTCCATCGACGCCAACGAA
				GGCGATATGCTCCTCGGATGGGATGTCGACCGTTTCCCAGCCAACGTCTATAGCGCCACC
				TTCGCCATGCTCGAAGTCATCAAAGCCGGTGGACTTACCGGTGGCTTCAACTTCGACGCC
				AAGACCCGCCGCGCTTCCAACACCTATGAAGATATGTTCAAGGCTTTCGTCCTTGGTATG
				GATACCTTCGCTTTAGGTCTTCTCAATGCCGAAGCCATCATCAAAGACGGCCGCATCGAC
				AAGTTCGTCGAGGATAGATATGCCAGCTTCAAGACCGGCATCGGTGCTAAGGTCCGCGAT
				CACTCCGCTACCCTTGAGGATTTAGCTGCCCACGCCCTTGAGACCAAGGTTTGCCCAGAT
				CCAGGCAGCGGCGACGAGGAAGAACTCCAGGAAATCCTCAACCAGTTAATGTTCGGTAAG
				AAATAA
1753MI35_004	Firmicutes	Amino	42	MEYFPEVESVQYKGPTSTEPFAFKYYDANRVVLGKPMKEWMPFAMAWWHNLGAAGTDMFG
		Acid		GNTMDKSWGVDKEKDPMGYAKAKVDAGFEFMQKMGIEYYCFHDVDLVPECDDITVMYQRL
				DEIGDYLLKKQKETGIKLLWSTANAFGHRRFMNGAGSSNSAEVYCFAAAQIKKALELCVK
				LGGKGYVFWGGREGYETLLNTDMKFEQENIANLMRCARDYGRKIGFKGDFYIEPKPKEPT
				KHQYDFDAATAIGFLRQYGLDKDFKMNIEANHATLAGHTFEHELRVSAMNGMLGSIDANE
				GDMLLGWDVDRFPANVYSATFAMLEVIKAGGLTGGFNFDAKTRRASNTYEDMFKAFVLGM
				DTFALGLLNAEAIIKDGRIDKFVEDRYASFKTGIGAKVRDHSATLEDLAAHALETKVCPD
				PGSGDEEELQEILNQLMFGKK
1754MI9_004	Firmicutes	DNA	43	ATGAGCGAATTTTTTAAGAATATTCCAGAGATTAAATTCGAAGGAAAAGATAGTAAAAAT
				CCATGGGCATTCAAGTATTACAATCCTGAATTGACCATTATGGGTAAAAAAATGTCTGAA
				CATCTTCCTTTTGCAATGGCCTGGTGGCATAACCTTGGCGCAAATGGAGTTGATATGTTC
				GGTTCGGGAACCGCCGATAAATCTTTCGGTCAGGCTCCGGGAACTATGGAGCACGCAAAG
				GCTAAGGTAGATGCAGGTATCGAGTTTATGAAGAAACTCGGAATCAAGTACTACTGCTGG
				CATGATGTAGACCTTGTTCCTGAAGATCCAAACGATATCAACGTAACAAACAAGCGCCTT
				GATGAGATTTCAGATTATATCCTTGAAAAAACAAAGGGAACTGACATCAAGTGTCTCTGG
				GGAACTGCTAACATGTTCAGTAATCCCCGCTTTATGAACGGGGCAGGCTCAACAAACTCT
				GCTGACGTTTACTGCTTTGCAGCTGCCCAGGTTAAAAAGGCTCTTGAGATTACCGTAAAG
				CTTGGTGGCCGCGGTTATGTATTCTGGGGTGGACGCGAAGGTTATGAAACTCTTCTTAAT
				ACAGATGTAAAGCTTGAACAGGAAAATATTGCAAACCTTATGCACATGGCAGTTGATTAT
				GGCCGTTCAATCGGTTTCAAGGGAGACTTCTACATCGAGCCTAAGCCAAAGGAGCCGATG
				AGTCATCAGTATGATTTTGATGCCGCAACTGCAATCGGCTTCCTCCGCCAGTATGGCCTC
				GACAAAGACTTTAAGATGAACATTGAGGCTAACCACGCTTCTCTTGCAAATCATACCTTC
				CAGCATGAGCTTTATATCAGCCGCATTAACGGAATGCTTGGTTCTGTAGATGCTAACCAG
				GGAAATCCAATTCTCGGCTGGGATACAGATAACTTCCCTTGGAATGTCTACGACGCAACT
				CTTGCAATGTACGAAGTACTCAAGGCTGGTGGACTTACAGGTGGCTTCAACTTTGACTCA
				AAGAACCGCCGCCCATCAAATACATTTGAAGATATGTTCCACGCTTACATCATGGGAATG
				GACACTTTTGCTCTTGGTCTTATTAAGGCTGCAGAAATTATTGAAGACGGAAGAATCGAT
				GGCTTCATTAAAGAAAAGTATTCAAGCTACGAAAGTGGAATTGGTAAGAAGATCCGCGAC
				AAGCAGACAACTTTGGAAGAGCTTGCTGCCCGTGCCGCAGAAATGAAAAAGCCATCTGAT
				CCAGGTTCAGGCCGCGAGGAATATCTGGAAGGAGTTGTTAACAATATCCTCTTTCGCGGA
				TAA
1754MI9_004	Firmicutes	Amino	44	MSEFFKNIPEIKFEGKDSKNPWAFKYYNPELTIMGKKMSEHLPFAMAWWHNLGANGVDMF
		Acid		GSGTADKSFGQAPGTMEHAKAKVDAGIEFMKKLGIKYYCWHDVDLVPEDPNDINVTNKRL
				DEISDYILEKTKGTDIKCLWGTANMFSNPRFMNGAGSTNSADVYCFAAAQVKKALEITVK
				LGGRGYVFWGGREGYETLLNTDVKLEQENIANLMHMAVDYGRSIGFKGDFYIEPKPKEPM
				SHQYDFDAATAIGFLRQYGLDKDFKMNIEANHASLANHTFQHELYISRINGMLGSVDANQ
				GNPILGWDTDNFPWNVYDATLAMYEVLKAGGLTGGFNFDSKNRRPSNTFEDMFHAYIMGM
				DTFALGLIKAAEIIEDGRIDGFIKEKYSSYESGIGKKIRDKQTTLEELAARAAEMKKPSD
				PGSGREEYLEGVVNNILFRG
1754MI22_004	Firmicutes	DNA	45	ATGAGCGAGTTTTTTAAGAATATTCCTCAAATAAAATACGAAGGAAAAGATAGCAAAAAT
				CCCTGGGCATTCAAGTATTACAATCCTGAATTGACAATCATGGGTAAAAAGATGAGCGAA
				CATCTTCCATTCGCAATGGCATGGTGGCATAACCTTGGCGCAAACGGCGTTGATATGTTT
				GGTCAGGGAACAGCAGACAAGTCTTTCGGACAGATTCCTGGAACTATGGAGCATGCAAAG
				GCTAAGGTTGATGCTGGTATAGAGTTTATGAAGAAGCTCGGAATCAAATATTACTGCTGG
				CACGATGTTGACCTTGTTCCTGAGGATCCAAACGATATCAACGTAACTAACAAACGTCTG
				GACGAAATTTCAGATTACATCCTTGAAAAGACAAAAGGAACAGACATTAAGTGTCTCTGG
				GGAACTGCAAACATGTTCGGTAACCCTCGCTTTATGAACGGTGCAGGCTCTACAAACTCT
				GCTGACGTTTACTGTTTTGCTGCCGCTCAGGTAAAAAAGGCTCTTGAGATTACTGTAAAG
				CTTGGTGGCCGAGGTTATGTTTTCTGGGGTGGCCGCGAAGGTTACGAAACTCTTCTCAAT
				ACAGACGTAAAACTTGAACAGGAAAATATCGCAAACCTCATGCATATGGCTGTTGATTAT
				GGCCGCTCAATCGGTTTCAAGGGAGACTTCTACATCGAGCCTAAGCCAAAGGAGCCAATG
				AGCCATCAGTATGATTTTGATGCTGCAACAGCAATCGGCTTCCTCCGCCAGTATGGCCTC
				GACAAAGATTTTAAGATGAACATCGAAGCTAACCATGCCTCACTTGCAAATCACACCTTC
				CAGCACGAGCTTTGTATCAGCCGCATAAACGGAATGCTTGGTTCTGTAGATGCAAATCAG
				GGAAATCCAATTCTTGGCTGGGATACAGATAACTTCCCATGGAATGTTTACGATGCAACT
				CTGGCAATGTACGAAGTTCTCAAGGCTGGCGGTCTAACAGGTGGCTTCAACTTTGACTCA
				AAGAACCGTCGCCCATCAAATACTTTTGAAGATATGTTCCACGCTTATATCATGGGTATG
				GATACTTTTGCCCTTGGCCTTATTAAGGCTGCAGAAATTATTGAAGACGGCAGAATTGAC
				GGCTTCATCAAAGAAAAGTATTCAAGCTTTGAAAGTGGAATTGGTAAGAAGATTCGTGAC
				AAGCAGACAAGTTTGGAAGAGCTTGCAGCTCGTGCCGCTGAAATGAAAAAGCCATCTGAT
				CCAGGTTCAGGCCGCGAGGAATACCTCGAAGGAGTTGTTAACAACATCCTCTTTCGCGGA
				TAA
1754MI22_004	Firmicutes	Amino	46	MSEFFKNIPQIKYEGKDSKNPWAFKYYNPELTIMGKKMSEHLPFAMAWWHNLGANGVDMF
		Acid		GQGTADKSFGQIPGTMEHAKAKVDAGIEFMKKLGIKYYCWHDVDLVPEDPNDINVTNKRL
				DEISDYILEKTKGTDIKCLWGTANMEGNPRFMNGAGSTNSADVYCFAAAQVKKALEITVK
				LGGRGYVFWGGREGYETLLNTDVKLEQENIANLMHMAVDYGRSIGFKGDFYIEPKPKEPM
				SHQYDFDAATAIGFLRQYGLDKDFKMNIEANHASLANHTFQHELCISRINGMLGSVDANQ
				GNPILGWDTDNFPWNVYDATLAMYEVLKAGGLTGGENFDSKNRRPSNTFEDMFHAYIMGM
				DTFALGLIKAAEIIEDGRIDGFIKEKYSSFESGIGKKIRDKQTSLEELAARAAEMKKPSD
				PGSGREEYLEGVVNNILFRG
727MI1_002	Firmicutes	DNA	47	ATGATATTTGAAAATATTCCCGCAATTCCTTATGAGGGTCCGAAGAGCACAAATCCGCTG
				GCGTTTAAATTCTATGATCCGGACAAGATCGTTATGGGAAAGCCCATGAAGGAGCATCTG
				CCCTTTGCAATGGCCTGGTGGCACAACCTTGGCGCGGCCGGAACCGATATGTTCGGGCGC
				GATACCGCCGACAAATCCTTCGGTGCGGTAAAAGGCACAATGGAGCATGCCAAAGCGAAA
				GTCGATGCCGGCTTTGAGTTCATGCAGAAGCTGGGGATCCGCTATTTCTGCTTCCATGAT
				GTGGATCTTGTTCCGGAGGCGGATGATATAAAGGAGACCAACCGCCGTCTGGACGAGATC
				AGCGATTACATCCTTGAAAAGATGAAGGGCACCGATATCAAGTGCCTTTGGGGCACGGCC
				AATATGTTCTCAAATCCGCGCTTTATGAACGGCGCAGGCTCCTCCAATTCTGCCGATGTA
				TTCGCTTTTGCGGCAGCACAGGCCAAGAAGGCCTTGGATCTGACCGTCAAACTCGGCGGG
				CGCGGCTATGTCTTCTGGGGCGGACGTGAGGGCTATGAGACACTTCTCAATACCGACATG
				AAGTTCGAGCAGGAGAATATCGCGAAGCTCATGCATATGGCTGTCGATTACGGCCGCAGC
				ATAGGCTTTACCGGTGATTTCTATATCGAGCCCAAACCGAAAGAGCCGATGAAACACCAG
				TATGATTTCGATGCAGCCACTGCGATAGGCTTCCTCCGCCAGTACGGACTCGATAAGGAC
				TTCAAGCTCAACATCGAGGCAAACCACGCCACACTGGCAGGTCACACTTTCCAGCACGAT
				CTGCGTGTTTCCGCAATAAACGGAATGCTGGGCAGCATTGACGCCAACCAGGGCGATATG
				CTCCTCGGCTGGGATACCGACGAGTTCCCGTTCAATGTATATGATGCGACCATGTGCATG
				TATGAGGTGCTCAAGTCAGACGGGCTCACCGGCGGCTTTAACTTCGACTCCAAATCACGC
				CGCCCGAGCTATACGGTCGAGGATATGTTTACAAGCTATATCCTCGGCATGGACACTTTT
				GCCCTCGGCCTTCTGAAAGCGGCCGAGCTTATCGAAGACGGAAGGCTTGACGCCTTCGTC
				AAAGAACGCTATTCAAGCTATGAGAGCGGCATCGGCGCAAAGATCCGCAGCGGAGAAACC
				GATTTGAAGGAATTGGCGGAATATGCGGACTCCCTCGGAGCCCCCGAACTTCCGGGCAGC
				GGAAAACAGGAACAGCTCGAGAGCATAGTAAATCAGATACTTTTCGGATAA
727MI1_002	Firmicutes	Amino	48	MIFENIPAIPYEGPKSTNPLAFKFYDPDKIVMGKPMKEHLPFAMAWWHNLGAAGTDMFGR
		Acid		DTADKSFGAVKGTMEHAKAKVDAGFEFMQKLGIRYFCFHDVDLVPEADDIKETNRRLDEI
				SDYILEKMKGTDIKCLWGTANMFSNPRFMNGAGSSNSADVFAFAAAQAKKALDLTVKLGG
				RGYVFWGGREGYETLLNTDMKFEQENIAKLMHMAVDYGRSIGFTGDFYIEPKPKEPMKHQ
				YDFDAATAIGFLRQYGLDKDFKLNIEANHATLAGHTFQHDLRVSAINGMLGSIDANQGDM
				LLGWDTDEFPFNVYDATMCMYEVLKSDGLTGGFNFDSKSRRPSYTVEDMFTSYILGMDTF
				ALGLLKAAELIEDGRLDAFVKERYSSYESGIGAKIRSGETDLKELAEYADSLGAPELPGS
				GKQEQLESIVNQILFG
727MI9_005	Firmicutes	DNA	49	ATGAGCGAGTTTTTTGCCAGCATTCCCAAAATTCCCTTTGAAGGCAAGGACAGCGCCAAT
				CCCCTGGCGTTCAAATACTACGACGCCGACAGGATGATACTGGGCAAGCCCATGAAGGAG
				CACCTTCCCTTCGCCATGGCCTGGTGGCACAACCTGTGCGCCGCGGGCACCGATATGTTT
				GGCCGGGACACCGCCGACAAGTCCTTCGGCCAGGTCAAGGGCACCATGGAACACGCCAAG
				GCCAAGGTGGACGCGGGCTTTGAGTTCATGAAGAAGCTGGGCATCCGCTACTTCTGCTTC
				CACGACGTGGACATCGTGCCCGAAGCCGACGACATCAAGGAAACCAACCGCCGTCTGGAC
				GAGATCTCCGACTATATCCTGGAGAAAATGAAAGGCACCGACATCCAGTGCCTGTGGGGC
				ACCGCCAACATGTTCGGCAACCCCCGCTATATGAACGGCGCGGGCAGCTCCAACTCCGCC
				GACGTATACTGCTTCGCCGCGGCCCAGATCAAAAAGGCCCTGGACATCACCGTGAAGCTG
				GGCGGCAAGGGCTACGTGTTCTGGGGCGGCCGCGAGGGCTACGAGACCCTGCTGAACACC
				GATATGAAGTTCGAGCAGGAGAACATCGCCCGCCTGATGCACATGGCCGTGGACTACGGC
				CGCAGCATCGGCTTCACCGGCGATTTCTACATCGAGCCCAAGCCCAAGGAGCCCATGAAG
				CACCAGTACGACTTCGACGCCGCCACCGCCATAGGCTTTTTGCGCCAGTACGGCCTGGAC
				AAGGATTTCAAGCTGAACATCGAGTCCAACCACGCCACCCTGGCGGGCCATACCTTCCAG
				CACGACCTGCGCGTTTCCGCCATCAACGGCATGCTGGGCTCCATCGACGCCAACCAGGGC
				GACTACCTGCTGGGCTGGGATACCGACGAGTTCCCCTACAGCGTATACGAGACCACCATG
				TGCATGTACGAGGTGCTCAAGGCCGGAGGTCTCACCGGCGGCTTCAATTTCGACGCCAAG
				AACCGCCGTCCCAGCTACACCCCCGAGGATATGTTCCACGCCTACATCCTTGGGATGGAC
				AGCTTCGCCCTGGGCCTGATCAAGGCCGCCGAGCTCATCGAGGACGGTCGCCTGGACGCC
				TTCGTCCGGGACCGCTACCAGAGCTGGGAGACCGGCATCGGCGATAAGATCCGCAAGGGC
				GAGACCACACTGGCCGAGCTGGCCGAGTACGCCGCCCGGATGGGCGCGCCCGCGCTGCCC
				GGCAGCGGCCGCCAGGAATACCTGGAGGGCGTGGTCAACAATATCCTGTTCAAATAA
727MI9_005	Firmicutes	Amino	50	MSEFFASIPKIPFEGKDSANPLAFKYYDADRMILGKPMKEHLPFAMAWWHNLCAAGTDMF
		Acid		GRDTADKSFGQVKGTMEHAKAKVDAGFEFMKKLGIRYFCFHDVDIVPEADDIKETNRRLD
				EISDYILEKMKGTDIQCLWGTANMFGNPRYMNGAGSSNSADVYCFAAAQIKKALDITVKL
				GGKGYVFWGGREGYETLLNTDMKFEQENIARLMHMAVDYGRSIGFTGDFYIEPKPKEPMK
				HQYDFDAATAIGFLRQYGLDKDFKLNIESNHATLAGHTFQHDLRVSAINGMLGSIDANQG
				DYLLGWDTDEFPYSVYETTMCMYEVLKAGGLTGGFNFDAKNRRPSYTPEDMFHAYILGMD
				SFALGLIKAAELIEDGRLDAFVRDRYQSWETGIGDKIRKGETTLAELAEYAARMGAPALP
				GSGRQEYLEGVVNNILFK
727MI27_002	Firmicutes	DNA	51	ATGAAGACCTATTTCAAAAAAATCCCCGTGATCCCCTACGAGGGACCGAAGTCCCAGAAT
				CCGCTGTCGTTCAAATTCTATGACGCGGACCGCATCGTTCTCGGCAAGCCCATGAAGGAG
				CATCTGCCCTTCGCCATGGCCTGGTGGCACAATCTGGGTGCTGCCGGAACGGACATGTTC
				GGCCGCGATACCGCCGACAAGTCCTTCGGAGCGGAGAAGGGCACCATGGAGCATGCCAAG
				GCCAAGGTGGACGCTGGCTTCGAGTTTATGAAGAAGGTGGGCATCCGGTATTTCTGCTTC
				CATGACGTGGATCTGGTCCCGGAAGCGGACGACATCAAGGAGACCAACCGCCGTCTCGAT
				GAGATCAGCGACTACATCCTCAAGAAGATGAAGGGCACGGATATCAAGTGCCTCTGGGGC
				ACCGCCAACATGTTCGGCAATCCCCGGTTCATGAACGGCGCGGGCAGCTCCAACAGCGCG
				GACGTGTTCTGCTTTGCCGCGGCCCAGGTGAAGAAGGCCTTGGACATCACCGTCAAGCTG
				GGCGGCCGGGGCTATGTGTTCTGGGGCGGCCGTGAGGGGTATGAGTCCCTGCTGAACACG
				GACGTGAAGTTTGAGCAGGAGAACATCGCCAAGCTCATGCACCTTGCCGTGGACTACGGC
				CGCAGCATCGGCTTCACCGGCGATTTCTACATCGAGCCCAAGCCCAAGGAGCCCATGAAG
				CACCAGTACGACTTCGATGCCGCCACCGCCATCGGCTTCCTCAGGCAGTACGGCCTCGAT
				AAGGACTTCAAGATGAACATTGAAGCCAACCACGCGACCCTGGCCGGCCACACCTTCCAG
				CACGACCTCAGGATCAGCGCCATCAACGGGATGCTGGGCTCCATCGACGCCAACCAGGGC
				GACCTCCTGCTGGGATGGGACACCGACGAATTCCCCTTCAACGTCTATGAGGCCACCATG
				TGCATGTACGAGGTCCTCAAGGCCGGCGGCCTCACCGGCGGCTTCAACTTCGACTCAAAG
				AACCGCCGTCCCTCCTACACCATGGAGGATATGTTCCACGCCTACATCCTGGGCATGGAC
				ACCTTCGCCCTGGGTCTTCTCAAGGCCGCGGAGCTCATCGAGGACGGTCGGATCGACAAA
				TTCGTGGAGGAGCGCTACGCCAGCTACAAGACCGGCATCGGCGCCAAGATCCGTTCCGGC
				GAGACCACGCTTCAGGAGCTGGCCGCCTATGCCGACAAGTTGGGCGCGCCTGCCCTTCCC
				GGCAGCGGCCGTCAGGAGTACCTGGAGAGCATCGTCAACCAGGTGCTCTTCGGGATGTGA
727MI27_002	Firmicutes	Amino	52	MKTYFKKIPVIPYEGPKSQNPLSFKFYDADRIVLGKPMKEHLPFAMAWWHNLGAAGTDMF
		Acid		GRDTADKSFGAEKGTMEHAKAKVDAGFEFMKKVGIRYFCFHDVDLVPEADDIKETNRRLD
				EISDYILKKMKGTDIKCLWGTANMFGNPRFMNGAGSSNSADVFCFAAAQVKKALDITVKL
				GGRGYVFWGGREGYESLLNTDVKFEQENIAKLMHLAVDYGRSIGFTGDFYIEPKPKEPMK
				HQYDFDAATAIGFLRQYGLDKDFKMNIEANHATLAGHTFQHDLRISAINGMLGSIDANQG
				DLLLGWDTDEFPFNVYEATMCMYEVLKAGGLTGGFNFDSKNRRPSYTMEDMFHAYILGMD
				TFALGLLKAAELIEDGRIDKFVEERYASYKTGIGAKIRSGETTLQELAAYADKLGAPALP
				GSGRQEYLESIVNQVLFGM
1753MI2_006	Neocallimastigales	DNA	53	ATGGCTAAAGAGTATTTTCCAGAGATTGGCAAAATCAAGTTTGAAGGCAAGGACAGCAAA
				AACCCAATGGCTTTCCACTACTATGACCCCGAGAAGGTGATCATGGGCAAGCCTATGAAA
				GACTGGCTCCGCTTCGCTATGGCATGGTGGCACACCCTCTGCGCAGAAGGTGGCGACCAG
				TTCGGTGGCGGCACTAAGAAGTTCCCTTGGAACAACGGCGCTGACGCTGTAGAAATCGCA
				AAACAGAAGGCTGACGCAGGTTTCGAAATCATGCAGAAGCTCGGCATCCCATATTTCTGC
				TTCCACGACGTGGACCTCGTGTCTGAGGGCGCATCTGTAGAAGAGTATGAGGCTAACCTC
				AAGGCTATCACAGACTACCTCGCTGTGAAGATGAAGGAAACAGGCATCAAGCTCCTGTGG
				TCTACTGCCAACGTATTCGGCAACGGCCGCTACATGAACGGTGCTTCTACCAACCCTGAC
				TTCGACGTCGTTGCTCGCGCTATCGTGCAGATTAAGAACGCTATCGACGCTGGTATCAAG
				CTCGGCGCTGAGAACTACGTGTTCTGGGGCGGACGCGAAGGCTACATGAGCCTCCTCAAC
				ACCGACCAGAAGCGTGAGAAGGAGCACATGGCCACTATGCTCACTATGGCTCGCGACTAC
				GCTCGCGCTAAGGGCTTCAAGGGCACATTCCTCATCGAGCCTAAGCCAATGGAGCCTTCT
				AAGCACCAGTATGACGTTGACACTGAGACTGTCATCGGCTTCCTCAAGGCACACAACCTC
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCAACTCTCGCTGGCCACACCTTC
				GAGCACGAGCTCGCAGTGGCAGTGGACAACAACATGCTCGGCTCTATCGACGCTAACCGT
				GGTGACTACCAGAATGGCTGGGATACTGACCAGTTCCCAATCGACCAGTACGAACTCGTT
				CAGGCTTGGATGGAAATCATCCGTGGCGGCGGTCTCGGCACTGGCGGCACGAACTTCGAC
				GCTAAGACTCGTCGTAACTCTACCGACCTCGAAGACATCTTCATCGCACACATCGCAGGC
				ATGGACGCTATGGCACGCGCACTCGAATCAGCTGCTAAGCTCCTCGAAGAGTCTCCATAC
				AAGGCAATGAAGGCAGCTCGCTACGCTTCATTCGACAACGGTATCGGTAAGGACTTCGAA
				GATGGCAAGCTCACTCTCGAGCAGGCTTACGAATACGGTAAGAAGGTTGGTGAGCCTAAG
				CAGACTTCTGGCAAGCAGGAGCTCTACGAAGCCATCGTTGCAATGTACGCTTAA
1753MI2_006	Neocallimastigales	Amino	54	MAKEYFPEIGKIKFEGKDSKNPMAFHYYDPEKVIMGKPMKDWLRFAMAWWHTLCAEGGDQ
		Acid		FGGGTKKFPWNNGADAVEIAKQKADAGFEIMQKLGIPYFCFHDVDLVSEGASVEEYEANL
				KATTDYLAVKMKETGIKLLWSTANVFGNGRYMNGASTNPDFDVVARAIVQIKNAIDAGIK
				LGAENYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEPS
				KHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNNMLGSIDANR
				GDYQNGWDTDQFPIDQYELVQAWMEIIRGGGLGTGGTNFDAKTRRNSTDLEDIFIAHIAG
				MDAMARALESAAKLLEESPYKAMKAARYASFDNGIGKDFEDGKLTLEQAYEYGKKVGEPK
				QTSGKQELYEAIVAMYA
5586MI3_005	Neocallimastigales	DNA	55	ATGGCTAAAGAATTTTTCCCAGAGATTGGTAAAATCAAGTTCGAAGGCAAGGATTCAAAG
				AATCCAATGGCTTTCCATTACTATGATGCAGAGAAGGTAATCATGGGCAAACCCATGAAG
				GACTGGCTCCGTTTCGCTATGGCATGGTGGCACACACTCTGTGCAGAGGGCGGCGACCAG
				TTCGGTGGCGGTACGAAGAAGTTCCCTTGGAACGAGGGTGCTAATGCTGTCGAGATTGCT
				AAGCAGAAGGCTGACGCTGGTTTCGAAATCATGCAGAAGCTTGGCATTCCTTACTTCTGC
				TTCCACGATGTTGACCTCGTTTCTGAAGGCGCATCTGTTGAGGAGTATGAGGCCAACCTC
				AAGGCTATCACTGACTATCTCGCGGTGAAGATGAAGGAGACTGGCATTAAGCTCCTGTGG
				TCTACTGCCAACGTGTTCGGCAATGGCCGTTACATGAATGGTGCTTCCACCAACCCTGAC
				TTCGACGTTGTTGCTCGCGCCATCGTTCAGATTAAGAACGCTATCGATGCAGGTATCAAG
				CTCGGTGCTGAGAACTATGTGTTCTGGGGCGGTCGTGAAGGTTACATGAGCCTCCTGAAC
				ACAGACCAGAAGCGTGAGAAGGAGCACATGGCTACTATGCTCACTATGGCTCGCGACTAC
				GCTCGCAGCAAGGGCTTCAAGGGTACTTTCCTCATCGAGCCTAAGCCAATGGAGCCATCT
				AAGCACCAGTACGACGTTGACACAGAGACTGTTATCGGCTTCCTGAAGGCACACAACCTT
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCAACACTCGCTGGTCACACCTTC
				GAGCACGAGCTCGCTGTGGCTGTCGACAACAATATGCTTGGTTCTATCGATGCTAACCGC
				GGTGACTACCAGAATGGTTGGGATACGGACCAGTTCCCAATTGACCAGTACGAGCTCGTT
				CAGGCTTGGATGGAGATCATCCGTGGTGGCGGTCTCGGCACAGGTGGTACAAACTTCGAC
				GCTAAGACTCGTCGTAACTCTACCGACCTCGAGGACATTTTCATTGCTCACATCGCTGGT
				ATGGACGCTATGGCTCGCGCTCTTGAGTCAGCAGCTAAGCTCCTTGAGGAGTCTCCATAC
				AAGAAGATGAAGGCTGCCCGTTATGCTTCTTTCGACAGCGGCATGGGTAAGGACTTTGAG
				AACGGCAAGCTCACACTCGAACAGGTTTATGAGTATGGTAAGAAGGTAGGTGAGCCCAAG
				CAGACTTCTGGCAAGCAGGAGCTCTTCGAGGCAATCGTGGCCATGTACGCATAA
5586MI3_005	Neocallimastigales	Amino	56	MAKEFFPEIGKIKFEGKDSKNPMAFHYYDAEKVIMGKPMKDWLRFAMAWWHTLCAEGGDQ
		Acid		FGGGTKKFPWNEGANAVEIAKQKADAGFEIMQKLGIPYFCFHDVDLVSEGASVEEYEANL
				KAITDYLAVKMKETGIKLLWSTANVFGNGRYMNGASTNPDFDVVARAIVQIKNAIDAGIK
				LGAENYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEPS
				KHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNNMLGSIDANR
				GDYQNGWDTDQFPIDQYELVQAWMEIIRGGGLGTGGTNFDAKTRRNSTDLEDIFIAHIAG
				MDAMARALESAAKLLEESPYKKMKAARYASFDSGMGKDFENGKLTLEQVYEYGKKVGEPK
				QTSGKQELFEAIVAMYA
5586MI91_002	Neocallimastigales	DNA	57	ATGGCTAAAGAGTATTTTCCAGAGATTGGTAAAATCAAGTTTGAAGGCAAGGATTCCAAG
				AATCCAATGGCATTCCACTATTATGATGCAGAGAAAGTGATTATGGGTAAGCCTATGAAG
				GAGTGGCTCCGCTTTGCAATGGCATGGTGGCACACACTCTGTGCAGAGGGTGGCGACCAG
				TTTGGTGGTGGCACTAAGAAATTCCCATGGAACGAGGGCACTGACGCTGTGACGATTGCT
				AAGCAGAAGGCTGATGCAGGTTTCGAAATCATGCAGAAACTCGGTTTCCCATATTTTTGC
				TTCCACGACATTGACCTCGTTTCCGAAGGCAACAGCATTGAAGAGTATGAGGCTAACCTC
				CAGGCAATCACTGATTATCTGAAAGTGAAGATGGAAGAGACAGGCATCAAACTCTTGTGG
				TCAACTGCCAACGTATTCGGCAATGGTCGCTACATGAATGGTGCTTCCACAAACCCAGAC
				TTTGACGTGGTGGCTCGTGCCATCGTTCAGATTAAGAACGCAATTGACGCTGGTATCAAA
				CTCGGTGCTGAGAACTATGTATTCTGGGGCGGTCGCGAAGGCTACATGAGCCTTCTGAAC
				ACTGACCAGAAGCGTGAGAAGGAGCACATGGCAACCATGCTCACTATGGCTCGCGACTAC
				GCTCGCAGCAAGGGTTTCAAGGGCACTTTCCTCATTGAGCCAAAGCCAATGGAGCCATCT
				AAGCACCAGTATGACGTTGACACGGAGACTGTCATCGGCTTCCTCAAGGCACACAACCTC
				GACAAGGATTTCAAGGTGAACATCGAAGTGAACCACGCTACACTTGCAGGTCATACTTTC
				GAGCACGAACTTGCTGTGGCTGTTGACAATGGCATGCTCGGTTCTATCGACGCTAACCGT
				GGTGACTATCAGAACGGTTGGGACACTGACCAGTTCCCAATCGACCAGTACGAACTCGTT
				CAGGCTTGGATGGAAATCATCCGTGGTGGTGGTCTCGGCACAGGTGGTACTAACTTCGAT
				GCTAAGACTCGTCGTAACTCAACTGACCTCGAGGACATCTTCATCGCACACATCTCTGGT
				ATGGATGCAATGGCACGTGCTCTCGAATCGGCGGCTAAACTTCTTGAGGAGTCTCCATAC
				TGCGCTATGAAGAAGGCTCGTTACGCTTCCTTCGACAGCGGCATCGGTAAGGACTTCGAG
				GACGGCAAACTCACGCTCGAGCAGGCTTACGAGTACGGCAAGAAAGTCGGCGAACCCAAG
				CAGACTTCTGGCAAGCAGGAACTCTACGAGGCAATCGTTGCCATGTACGCATAA
5586MI91_002	Neocallimastigales	Amino	58	MAKEYFPEIGKIKFEGKDSKNEMAFHYYDAEKVIMGKPMKEWLRFAMAWWHTLCAEGGDQ
		Acid		FGGGTKKFPWNEGTDAVTIAKQKADAGFEIMQKLGFPYFCFHDIDLVSEGNSIEEYEANL
				QAITDYLKVKMEETGIKLLWSTANVFGNGRYMNGASTNPDFDVVARAIVQIKNAIDAGIK
				LGAENYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEPS
				KHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANR
				GDYQNGWDTDQFPIDQYELVQAWMEIIRGGGLGTGGTNFDAKTRRNSTDLEDIFIAHISG
				MDAMARALESAAKLLEESPYCAMKKARYASFDSGIGKDFEDGKLTLEQAYEYGKKVGEPK
				QTSGKQELYEAIVAMYA
5586MI194_003	Neocallimastigales	DNA	59	ATGGCAAAAGAGTATTTCCCTACGATCGGTAAGATCGTTTATGAAGGACCGGAGTCCAAG
				AACCCTATGGCATTTCATTACTATGACGCAGAGCGCGTAGTAGCTGGTAAAAAAATGAAA
				GATTGGATGCGTTTCGCTATGGCATGGTGGCACACCCTCTGTGCAGAAGGTGCAGACCAG
				TTCGGTGGAGGCACCAAACACTTCCCGTGGAGTGAAGGTCCCGATGCCGTAACCATCGCC
				AAGCAGAAAGCAGACGCAGGTTTTGAGATCATGCAGAAACTCGGCTTCCCGTATTTCTGT
				TTCCATGACGTGGATCTGGTCAGCGAAGGCAGCAGCGTAGAAGAGTACGAGGCGAACCTC
				GCAGCCATCACCGATTATCTCAAGCAGAAAATGGACGAGTCGGGTATCAAACTCCTTTGG
				TCCACTGCTAACGTATTCGGTCACGCCCGTTACATGAACGGTGCCAGCACCAATCCTGAC
				TTTGATGTCGTTGCCCGTGCGATTGTGCAGATCAAGAATGCTATCGACGCAGGTATCAAA
				CTCGGCGCAGAGAACTACGTCTTCTGGGGCGGTCGTGAAGGTTATATGAGCCTGCTCAAT
				ACCGACCAGAAACGCGAGAAAGAGCATACGGCAATGATGCTGCGTATGGCGCGTGACTAT
				GCCCGCAGCAAAGGTTTCAAAGGTACCTTCCTCATCGAACCCAAACCCATGGAGCCGTCC
				AAGCACCAGTATGACGTAGATACCGAGACGGTGATAGGTTTCCTCAAAGCACACGGTTTG
				GAGAAAGACTTTAAGGTAAACATCGAAGTGAACCACGCTACCCTCGCCGGTCACACTTTC
				GAGCACGAACTGGCAGTAGCCGTAGATAACGGCATGCTCGGTTCGATCGATGCCAACCGC
				GGTGACTATCAGAACGGATGGGATACCGACCAGTTCCCCATCGATAACTTCGAACTGACC
				CAAGCATGGATGCAGATCGTACGTAACGGTGGTCTCGGCACAGGCGGAACGAACTTCGAC
				TCCAAGACCCGTCGTAACTCCACCGATCTCGAGGATATCTTCATCGCTCACATCAGTGGT
				ATGGACGCTTGTGCCCGTGCCCTATTGAATGCCGTAGAGATCATGGAGAAATCACCGATC
				CCTGCTATGCTCAAAGAGCGTTACGCTTCCTTCGATAGCGGTCTGGGTAAAGATTTCGAG
				GACGGCAAACTGACCCTTGAGCAAGTCTATGAGTACGGTAAGAAAGTAGGCGAACCCAAA
				CAAACCAGCGGCAAACAAGAACTCTATGAGGCTATCGTTGCCCTCTACGCTAAATAA
5586MI194_003	Neocallimastigales	Amino	60	MAKEYFPTIGKIVYEGPESKNPMAFHYYDAERVVAGKKMKDWMRFAMAWWHTLCAEGADQ
		Acid		FGGGTKHFPWSEGPDAVTIAKQKADAGFEIMQKLGFPYFCFHDVDLVSEGSSVEEYEANL
				AAITDYLKQKMDESGIKLLWSTANVFGHARYMNGASTNPDFDVVARAIVQIKNAIDAGIK
				LGAENYVFWGGREGYMSLLNTDQKREKEHTAMMLRMARDYARSKGFKGTFLIEPKPMEPS
				KHQYDVDTETVIGFLKAHGLEKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANR
				GDYQNGWDTDQFPIDNFELTQAWMQIVRNGGLGTGGTNFDSKTRRNSTDLEDIFIAHISG
				MDACARALLNAVEIMEKSPIPAMLKERYASFDSGLGKDFEDGKLTLEQVYEYGKKVGEPK
				QTSGKQELYEAIVALYAK
5586MI198_003	Neocallimastigales	DNA	61	ATGAAAGAGTATTTCCCTGAGATCGGTAAGATCCAATTTGAAGGCCCGGAGTCCAAGAAC
				CCGATGGCATTTCACTACTATGACGCAGAGCGCGTCGTAGCCGGTAAAACAATGAAAGAG
				TGGATGCGTTTCGCTATGGCTTGGTGGCACACCCTCTGTGCGGAAGGCGGCGACCAGTTC
				GGAGGCGGAACGAAGAAGTTCCCCTGGAACGAAGGCGCTAACGCTTTGGAGATCGCCAAG
				CACAAAGCCGATGCGGGATTTGAGATCATGCAGAAACTCGGCATCCCTTATTTCTGTTTC
				CATGACGTGGATCTCATCGCCGAGGGCGGTTCGGTAGAAGAGTACGAAGCCAACCTCGCT
				GCCATCACCGATTACCTCAAACAGAAAATGGACGAGACTGGCATCAAACTGCTGTGGTCC
				ACGGCGAACGTCTTCAGCAACCCCCGTTATATGAACGGCGCCAGCACGAACCCCGATTTC
				GATGTAGTAGCGCGTGCCATCGTCCAGATCAAGAACGCTATCGACGCCGGTATCAAACTC
				GGAGCAGAGAACTATGTCTTCTGGGGTGGTCGCGAGGGCTATATGAGCCTCCTCAACACT
				GACCAGCGCCGAGAGAAAGAGCATATGGCTACCATGCTCCGTATGGCGCGTGACTACGCG
				CGTGCCAAAGGATTCAAGGGCACCTTCCTCATCGAACCCAAACCATGTGAGCCGTCCAAA
				CATCAGTATGATGTCGATACCGAGACCGTCATCGGTTTCCTCAAAGCGCATGGACTCGAC
				AAGGATTTCAAAGTCAATATCGAGGTCAACCACGCCACCCTCGCAGGCCACACGTTCGAA
				CACGAACTGGCTTGCGCTGTAGATGCCGGCATGCTCGGTTCGATTGACGCCAACCGCGGT
				GACGCCCAGAACGGATGGGACACCGACCAGTTCCCTATTGATAACTTCGAACTCACACAG
				GCTTTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCC
				AAGACACGCCGTAACTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATG
				GACGCTTGCGCACGTGCGTTACTCAATGCTGTCGAAATCCTCGAGAAGAGCCCGATTCCG
				GCGATGCTCAAAGAGCGTTATGCTTCCTTTGACGGCGGCATCGGAAAGGACTTCGAGGAG
				GGAAAACTGACTTTCGAGCAGGTCTATGAGTACGGCAAGAAAGTCGGCGAACCCAAACAG
				ACCAGCGGCAAACAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAATAG
5586MI198_003	Neocallimastigales	Amino	62	MKEYFPEIGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGGDQF
		Acid		GGGTKKFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGGSVEEYEANLA
				AITDYLKQKMDETGIKLLWSTANVFSNPRYMNGASTNPDFDVVARAIVQIKNAIDAGIKL
				GAENYVFWGGREGYMSLLNTDQRREKEHMATMLRMARDYARAKGFKGTFLIEPKPCEPSK
				HQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRG
				DAQNGWDTDQFPIDNFELTQAFMQIVRNGGEGTGGTNEDAKTRRNSTDLEDIFIAHISGM
				DACARALLNAVEILEKSPIPAMLKERYASFDGGIGKDFEEGKLTFEQVYEYGKKVGEPKQ
				TSGKQELYETIVALYAK
5586MI201_003	Neocallimastigales	DNA	63	ATGGCAAAAGAGTATTTCCCTACGATCGGTAAGATCGTTTATGAAGGACCGGAATCCAAG
				AACCCTATGGCATTTCATTACTATGACGCAGAGCGCGTAGTAGCTGGTAAAAAAATGAAA
				GATTGGATGCGTTTCGCTATGGCATGGTGGCACACCCTCTGTGCAGAAGGTGCAGACCAG
				TTCGGTGGAGGCACCAAACACTTCCCGTGGAATGAAGGTCCCGATGCCGTAACCATCGCC
				AAGCAGAAAGCAGACGCAGGTTTTGAGATCATGCAGAAACTCGGCTTCCCGTATTTCTGT
				TTCCATGACGTGGATCTGGTCGGCGAAGGCAGCAGCGTAGAAGAGTACGAGGCGAACCTC
				GCAGCCATCACCGATTATCTCAAGCAGAAAATGGACGAGTCGGGTATCAAACTCCTTTGG
				TCCACTGCTAACGTATTCGGTCACGCCCGTTACATGAACGGTGCCAGCACCAATCCTGAC
				TTTGATGTCGTTGCCCGTGCGATTGTGCAGATCAAGAATGCTATCGACGCAGGTATCAAA
				CTCGGCGCAGAGAACTACGTCTTCTGGGGCGGTCGTGAAGGTTATATGAGCCTGCTCAAC
				ACCGACCAGAAACGCGAGAAAGAGCATACGGCAATGATGCTGCGTATGGCGCGTGACTAT
				GCCCGCAGCAAAGGTTTCAAAGGTACCTTCCTCATCGAACCCAAACCCATGGAGCCGTCC
				AAGCACCAGTATGACGTAGATACCGAGACGGTGATAGGTTTCCTCAAAGCACACGGTTTG
				GAGAAAGACTTTAAGGTAAACATCGAAGTGAACCACGCTACCCTCGCCGGTCACACTTTC
				GAGCACGAACTGGCAGTAGCCGTAGATAACGGCATGCTCGGTTCGATCGATGCCAACCGC
				GGTGACTATCAGAACGGATGGGATACCGACCAGTTCCCCATCGATAACTTCGAACTGACC
				CAAGCATGGATGCAGATCGTACGTAACGGTGGTCTCGGCACAGGCGGAACGAACTTCGAC
				TCCAAGACCCGTCGTAACTCCACCGATCTCGAGGATATCTTCATCGCTCACATCAGTGGT
				ATGGACGCTTGTGCCCGTGCCCTATTGAATGCCGTAGAGATCATGGAGAAATCACCGATC
				CCTGCTATGCTCAAAGAGCGTTACGCTTCCTTCGATAGCGGTCTGGGTAAAGATTTCGAG
				GACGGCAAACTGACCCTTGAGCAAGTCTATGAGTACGGTAAGAAAGTAGGCGAACCCAAA
				CAAACCAGCGGCAAACAAGAACTCTATGAGGCTATCGTTGCCCTCTACGCTAAATAA
5586MI201_003	Neocallimastigales	Amino	64	MAKEYFPTIGKIVYEGPESKNPMAFHYYDAERVVAGKKMKDWMRFAMAWWHTLCAEGADQ
		Acid		FGGGTKHFPWNEGPDAVTIAKQKADAGFEIMQKLGFPYFCFHDVDLVGEGSSVEEYEANL
				AAITDYLKQKMDESGIKLLWSTANVFGHARYMNGASTNPDFDVVARAIVQIKNAIDAGIK
				LGAENYVFWGGREGYMSLLNTDQKREKEHTAMMLRMARDYARSKGFKGTFLIEPKPMEPS
				KHQYDVDTETVIGFLKAHGLEKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANR
				GDYQNGWDTDQFPIDNFELTQAWMQIVRNGGLGTGGTNFDSKTRRNSTDLEDIFIAHISG
				MDACARALLNAVEIMEKSPIPAMLKERYASFDSGLGKDFEDGKLTLEQVYEYGKKVGEPK
				QTSGKQELYEAIVALYAK
5586MI204_002	Neocallimastigales	DNA	65	ATGAAAGAGTATTTCCCTGAGGTCGGTAAGATCCAATTTGAAGGCCCGGAGTCTAAGAAC
				CCGATGGCATTTCACTACTATGACGCAGAGCGCGTCGTAGCCGGTAAAACAATGAAAGAG
				TGGATGCGTTTCGCTATGGCTTGGTGGCACACCCTCTGTGCAGAAGGCGGCGACCAGTTC
				GGAGGCGGAACGAAGCATTTCCCGTGGAATGAAGGCGCTAACGCTTTGGAGATCGCCAAA
				CACAAAGCCGATGCGGGATTCGAGATCATGCAGAAACTCGGCATCCCCTATTTCTGTTTC
				CATGACGTGGATCTCATCGCCGAGGGCGGTTCGGTAGAAGAGTACGAAACCAACCTCGCT
				GCTATCACCGACTACCTCAAGCAGAAAATGGACGAGACCGGCATCAAACTGCTGTGGTCC
				ACGGCGAACGTGTTCAGCAACCCCCGTTATATGAACGGCGCGAGCACGAACCCCGATTTC
				GATGTAGTAGCGCGTGCCATCGTGCAGATCAAGAATGCCATCGACGCCGGCATCAAACTG
				GGCGCAGAGAACTATGTCTTCTGGGGCGGTCGCGAGGGCTACATGAGCCTGCTCAACACC
				GACCAGCGCCGCGAGAAAGAGCATATGGCTACTATGCTCCGTATGGCGCGTGACTACGCG
				CGTGCCAAAGGATTCAAGGGCACCTTTCTCATCGAACCCAAACCGTGTGAGCCGTCCAAA
				CATCAGTATGATGTCGATACCGAGACCGTCATCGGTTTCCTCAAAGCGCATGGACTCGAC
				AAGGATTTCAAGGTTAATATCGAGGTCAACCACGCCACCCTCGCAGGCCACACGTTCGAA
				CACGAACTGGCTTGCGCTGTAGATGCCGGCATGCTCGGTTCGATTGACGCCAACCGCGGT
				GACGCCCAGAACGGATGGGACACCGACCAGTTCCCTATTGATAACTTCGAACTCACACAG
				GCTTTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCC
				AAGACACGCCGTAACTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATG
				GACGCTTGCGCACGTGCGTTGCTCAACGCCATCGAAATCCTCGAGAAGAGCCCGATCCCG
				GCTATGCTCAAAGACCGTTATGCCTCCTTTGATGGCGGCATCGGAAAGGACTTTGAGGAG
				GGCAAACTGACTTTCGAGCAGGTCTATGAGTACGGCAAGAAGGTCGGAGAACCCAAACAG
				ACCAGCGGCAAACAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAATAG
5586MI204_002	Neocallimastigales	Amino	66	MKEYFPEVGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGGDQF
		Acid		GGGTKHFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGGSVEEYETNLA
				AITDYLKQKMDETGIKLLWSTANVFSNPRYMNGASTNPDFDVVARAIVQIKNAIDAGIKL
				GAENYVFWGGREGYMSLLNTDQRREKEHMATMLRMARDYARAKGFKGTFLIEPKPCEPSK
				HQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRG
				DAQNGWDTDQFPIDNFELTQAFMQIVRNGGFGTGGTNFDAKTRRNSTDLEDIFIAHISGM
				DACARALLNAIEILEKSPIPAMLKDRYASFDGGIGKDFEEGKLTFEQVYEYGKKVGEPKQ
				TSGKQELYETIVALYAK
5586MI207_002	Neocallimastigales	DNA	67	ATGAAAGAGTATTTCCCTGAGATCGGTAAGATGCAATTTGAAGGCCCGGAGTCCAAGAAC
				CCGATGGCGTTTCACTACTATGACGCTGAGCGCGTCGTAGCCGGTAAAACAATGAAAGAG
				TGGATGCGTTTCGCTATGGCTTGGTGGCACACCCTCTGTGCGGAAGGCGGCGACCAGTTC
				GGAGGAGGAACGAAGAAATTCCCCTGGAACGAAGGGGCAAACGCTTTGGAGATCGCCAAG
				CACAAAGCCGATGCGGGATTCGAGATCATGCAGAAACTCGGCATCCCTTATTTCTGTTTC
				CATGACGTGGATCTCATCGCCGAGGGCGAATCGGTAGAAGAGTACGAAGCCAACCTCGCT
				GCCATCACCGATTACCTCAAACAGAAAATGGACGAGACCGGCATCAAACTGCTGTGGTCC
				ACGGCGAACGTGTTCAGCAACCCCCGTTATATGAACGGCGCCAGCACGAACCCCGATTTC
				GATGTAGTGGCACGCGCTATCGTACAAATCAAGAACGCTATCGACGCCGGTATCAAACTC
				GGAGCAGAGAACTATGTCTTCTGGGGCGGTCGCGAGGGCTATATGTCGCTCCTCAACACC
				GACCAGCGCCGAGAGAAAGAGCATATGGCTACTATGCTCCGTATGGCGCGTGACTACGCG
				CGTTCCAAAGGATTCAAGGGCACCTTCCTCATCGAACCCAAACCGTGTGAGCCGTCCAAA
				CATCAGTACGATGTGGACACAGAGACCGTCATCGGTTTCCTTAAAGCGCATGGACTCGAC
				AAGGATTTCAAAGTCAATATCGAGGTCAACCACGCCACCCTCGCAGGCCACACGTTCGAA
				CACGAACTGGCTTGCGCTGTAGATGCCGGCATGCTCGGTTCGATTGACGCCAACCGCGGT
				GACGCCCAGAACGGATGGGACACCGACCAATTCCCTATTGATAACTTCGAACTCACTCAG
				GCTTTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCC
				AAGACACGCCGTAACTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATG
				GACGCTTGCGCTCGTGCGTTGCTCAATGCTGTCGAAATCCTCGAGAAGAGCCCGATCCCG
				GCTATGCTCAAAGAGCGTTATGCTTCCTTTGACGGCGGCATCGGAAAGGACTTTGAGGAG
				GGCAAACTGACTTTCGAGCAGGTCTATGAGTACGGCAAGAAGGTCGGAGAACCCAAACAG
				ACCAGCGGCAAACAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAATGA
5586MI207_002	Neocallimastigales	Amino	68	MKEYFPEIGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGGDQF
		Acid		GGGTKKFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGESVEEYEANLA
				AITDYLKQKMDETGIKLLWSTANVFSNPRYMNGASTNPDFDVVARAIVQIKNAIDAGIKL
				GAENYVFWGGREGYMSLLNTDQRREKEHMATMLRMARDYARSKGFKGTFLIEPKPCEPSK
				HQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRG
				DAQNGWDTDQFPIDNFELTQAFMQIVRNGGFGTGGTNFDAKTRRNSTDLEDIFIAHISGM
				DACARALLNAVEILEKSPIPAMLKERYASFDGGIGKDFEEGKLTFEQVYEYGKKVGEPKQ
				TSGKQELYETIVALYAK
5586MI209_003	Neocallimastigales	DNA	69	ATGAAAGAGTATTTCCCTGAGATCGGTAAGATCCAATTTGAAGGCCCGGAGTCCAAGAAC
				CCGATGGCGTTTCACTACTATGACGCAGAGCGCGTAGTAGCCGGTAAAACAATGAAAGAA
				TGGATGCGTTTCGCCATGGCATGGTGGCACACCCTCTGTGCAGAAGGCGGCGACCAGTTC
				GGAGGAGGAACGAAGCATTTCCCGTGGAATGAAGGCGCTAACGCTTTGGAGATCGCCAAA
				CACAAAGCCGATGCGGGATTCGAGATCATGCAGAAACTCGGCATCCCCTATTTCTGTTTC
				CATGACGTGGATCTCATCGCCGAGGGCGATTCGGTGGAGGAGTACGAAGCTAACCCCGCT
				GCCATCACCGATTACCTCAAACAGAAAATGGACGAGACCGGCATCAAACTGCTGTGGTCC
				ACGGCGAACGTCTTCAGCAACCCCCGTTACATGAACGGTGCGAGCACGAACCCGGATTTC
				GATGTAGTGGCACGCGCTATCGTACAAATCAAGAACGCTATCGACGCCGGTATCAAACTC
				GGAGCAGAGAACTATGTCTTCTGGGGCGGTCGCGAGGGCTATATGTCGCTCCTCAACACC
				GACCAGCGTCGCGAGAAAGAGCATATGGCTACTATGCTCCGTATGGCGCGTGACTACGCG
				CGTGCCAAAGGATTCAAGGGCACCTTCCTCATCGAACCCAAACCATGTGAGCCGTCCAAA
				CATCAGTACGATGTGGACACAGAGACTGTCATCGGTTTCCTCAAAGCGCATGGACTCGAC
				AAGGATTTCAAAGTCAACATCGAGGTCAACCACGCCACCCTCGCAGGTCACACGTTCGAA
				CACGAACTGGCTTGCGCTGTAGATGCCGGCATGCTCGGTTCGATTGACGCCAACCGCGGT
				GACGCCCAGAACGGATGGGACACTGACCAGTTCCCTATTGATAACTTCGAACTCACACAG
				GCTTTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCC
				AAGACACGCCGTAACTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATG
				GACGCTTGTGTCCGTGCGTTGCTCAACGCCATCGAAATCCTCGAGAAGAGCCCGATCCCG
				GCTATGCTCAAAGAGCGTTACGCTTCCTTTGACGGCGGCATCGGAAAGGACTTTGAGGAT
				GGTAAACTGACTTTCGAGCAGGTCTATGAGTACGGCAAGAAGGTCGGAGAACCCAAACAG
				ACCAGCGGCAAACAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAGTAA
5586MI209_003	Neocallimastigales	Amino	70	MKEYFPEIGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGGDQF
		Acid		GGGTKHFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGDSVEEYEANPA
				AITDYLKQKMDETGIKLLWSTANVFSNPRYMNGASTNPDFDVVARAIVQIKNAIDAGIKL
				GAENYVFWGGREGYMSLLNTDQRREKEHMATMLRMARDYARAKGFKGTFLIEPKPCEPSK
				HQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRG
				DAQNGWDTDQFPIDNFELTQAFMQIVRNGGFGTGGTNFDAKTRRNSTDLEDIFIAHISGM
				DACVRALLNAIEILEKSPIPAMLKERYASFDGGIGKDFEDGKLTFEQVYEYGKKVGEPKQ
				TSGKQELYETIVALYAK
5586MI214_002	Neocallimastigales	DNA	71	ATGAAAGAGTATTTCCCTGAGATCGGAAAGATCCAATTCGAAGGCCCGGAGTCCAAGAAT
				CCTATGGCATTTCACTACTATGACGCAGAGCGTGTAGTAGCCGGTAAAACAATGAAAGAG
				TGGATGCGTTTCGCTTTGGCATGGTGGCACACGCTCTGCGCAGAAGGCGGCGACCAGTTC
				GGAGGCGGCACGAAGCATTTCCCTTGGAATGAAGGTGCAAACGCTTTGGAGATCGCCAAG
				CACAAAGCCGATGCAGGCTTCGAGATCATGCAGAAACTCGGCATCCCCTATTTCTGTTTC
				CATGACGTGGATCTGATCGCCGAGGGCGGTTCGGTAGAAGAGTATGAAGCTAATTTAACG
				GCTATCACCGATTACCTCAAACAGAAAATGGACGAGACCGGCATCAAACTGCTGTGGTCC
				ACTGCGAACGTGTTCGGTAACGCACGTTATATGAACGGCGCGAGCACGAACCCCGATTTC
				GATGTAGTGGCACGCGCTATCGTGCAGATCAAGAACGCTATCGACGCCGGCATCAAACTG
				GGCGCAGAGAACTACGTCTTCTGGGGCGGTCGCGAGGGATATATGTCGCTCCTGAACACC
				GACCAGAAGCGTGAGAAAGAGCATATGGCTACCATGCTCCGTATGGCGCGTGACTACGCG
				CGTTCCAAAGGATTCAAAGGTACGTTCCTCATCGAGCCCAAACCGTGTGAGCCGTCCAAA
				CATCAGTACGACGTGGACACTGAGACCGTCATCGGTTTCCTCAAAGCCCATGGTCTCGGC
				AAGGATTTCAAAGTGAACATCGAGGTGAATCACGCCACCCTCGCAGGGCACACGTTCGAA
				CACGAACTGGCTTGCGCCGTAGATGCCGGCATGCTCGGTTCGATCGACGCCAACCGCGGT
				GACGCACAAAACGGATGGGACACCGACCAGTTCCCTATTGATAATTTCGAACTCACCCAG
				GCATTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCC
				AAGACACGCCGTAATTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATG
				GACGCTTGTGCCCGTGCGTTGCTCAATGCTGTCGAAATCCTTGAAAAGAGCCCGATCCCG
				GCGATGCTCAAAGAGCGTTACGCCTCCTTTGACAGCGGTATGGGTAAGGACTTTGAGGAG
				GGCAAGCTGACCTTCGAGCAGGTCTATGAGTACGGCAAACAGGTCGGCGAACCCAAACAG
				ACCAGCGGCAAGCAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAATAG
5586MI214_002	Neocallimastigales	Amino	72	MKEYFPEIGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFALAWWHTLCAEGGDQF
		Acid		GGGTKHFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGGSVEEYEANLT
				AITDYLKQKMDETGIKLLWSTANVFGNARYMNGASTNPDFDVVARAIVQIKNAIDAGIKL
				GAENYVFWGGREGYMSLLNTDQKREKEHMATMLRMARDYARSKGFKGTFLIEPKPCEPSK
				HQYDVDTETVIGFLKAHGLGKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRG
				DAQNGWDTDQFPIDNFELTQAFMQIVRNGGFGTGGTNFDAKTRRNSTDLEDIFIAHISGM
				DACARALLNAVEILEKSPIPAMLKERYASFDSGMGKDFEEGKLTFEQVYEYGKQVGEPKQ
				TSGKQELYETIVALYAK
5751MI3_001	Neocallimastigales	DNA	73	ATGAAAGAGTATTTTCCACAAATCGGCAAGATCCCATTTGAGGGACCAGAGTCAAAGAAC
				CCAATGGCATTCCACTACTATGACGCAGAGCGCGTAGTTGCCGGTAAGACAATGAAGGAA
				TGGATGCGTTTCGCTATGGCCTGGTGGCACACTCTCTGTGCTGAGGGTAGCGATCAGTTC
				GGCCCTGGTACAAAGAAGTTCCCTTGGAACGAGGGCGAGACAGCCCTTGAGCGCGCTAAG
				CACAAGGCAGATGCTGGCTTCGAGGTTATGCAGAAGCTCGGCATCCCATATTTCTGCTTC
				CACGATGTAGACCTTATCGACGAGGGTGCTAACGTGGCTGAGTATGAGGCAAACCTCGCT
				GCTATCACTGACTACCTGAAGGAGAAGATGGAGGAGACTGGCGTAAAGCTCCTCTGGTCT
				ACAGCCAACGTGTTCGGTAACGCTCGCTATATGAACGGTGCTTCTACAAATCCTGACTTC
				GACGTTGTGGCTCGTGCCATCGTACAGATTAAGAACGCTATCGACGCTGGTATCAAGCTT
				GGTGCTGAGAACTACGTGTTCTGGGGCGGCCGCGAGGGCTACATGAGCCTTCTGAACACT
				GACCAGAAGCGCGAGAAGGAGCACATGGCAACTATGCTCGGCATGGCTCGCGACTATGCC
				CGCGCTAAGGGATTCACCGGTACCTTCCTCATTGAGCCAAAGCCAATGGAGCCAACAAAG
				CATCAGTATGATGTTGACACAGAGACCGTTATCGGTTTCCTCAAGGCTCACGGTCTGGAC
				AAGGACTTCAAGGTGAACATCGAGGTGAACCACGCTACTCTCGCCGGTCACACCTTCGAG
				CACGAGCTCGCTTGCGCTGTTGACGCTGGTATGCTCGGTTCTATCGACGCTAACCGCGGT
				GACGCTCAGAACGGATGGGATACCGACCAGTTCCCAATCGACAACTTCGAGCTGACACAG
				GCTTGGATGCAGATTGTTCGCAATGGCGGTCTTGGCACAGGTGGTACCAACTTCGACGCA
				AAGACCCGTCGTAACTCTACCGACCTCGAGGACATCTTCATCGCTCACATCTCCGGTATG
				GACGCTTGTGCACGCGCTCTCCTCAACGCAGTAGAGATACTCGAGAACTCTCCAATCCCA
				ACAATGCTGAAGGACCGCTATGCAAGCTTCGACTCAGGTATGGGTAAGGACTTCGAGGAC
				GGCAAGCTCACACTTGAGCAGGTTTATGAGTATGGTAAGAAGGTCGACGAGCCAAAGCAG
				ACCTCTGGTAAGCAGGAACTCTATGAGACCATCGTTGCTCTCTATGCAAAATAA
5751MI3_001	Neocallimastigales	Amino	74	MKEYFPQIGKIPFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGSDQF
		Acid		GPGTKKFPWNEGETALERAKHKADAGFEVMQKLGIPYFCFHDVDLIDEGANVAEYEANLA
				AITDYLKEKMEETGVKLLWSTANVFGNARYMNGASTNPDFDVVARAIVQIKNAIDAGIKL
				GAENYVFWGGREGYMSLLNTDQKREKEHMATMLGMARDYARAKGFTGTFLIEPKPMEPTK
				HQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRG
				DAQNGWDTDQFPIDNFELTQAWMQIVRNGGLGTGGTNFDAKTRRNSTDLEDIFIAHISGM
				DACARALLNAVEILENSPIPTMLKDRYASFDSGMGKDFEDGKLTLEQVYEYGKKVDEPKQ
				TSGKQELYETIVALYAK
5753MI3_002	Prevotella	DNA	75	ATGCCTAAAGAATACTTCCCCTCCATCGGCAAAATCCCTTTTGAAGGAGGCGACAGCAAA
				AATCCCCTCGCTTTCCATTATTATGACGCCGGACGCGTGGTTATGGGCAAGCCCATGAAG
				GAATGGCTTAAATTCGCCATGGCCTGGTGGCACACGCTGGGCCAGGCCTCCGGAGACCCC
				TTCGGCGGCCAGACCCGCAGCTACGAATGGGACAAGGGCGAATGCCCCTACTGCCGCGCC
				AAAGCCAAGGCCGACGCCGGTTTTGAAATCATGCAAAAGCTGGGTATCGAATACTTCTGC
				TTCCACGATGTGGACCTTATCGAGGATTGCGATGACATTGCCGAATACGAAGCCCGCATG
				AAGGACATCACGGACTACCTGCTGGAAAAGATGAAGGAGACCGGCATCAAGAACCTCTGG
				GGCACCGCCAATGTCTTCGGCCACAAGCGCTACATGAACGGCGCCGGCACCAATCCGCAG
				TTCGATGTGGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAG
				CTGGGCGGCTCCAACTATGTGTTCTGGGGCGGCCGCGAAGGCTATTACACCCTCCTCAAC
				ACCCAGATGCAGCGGGAAAAAGACCACCTGGCCAAGTTGCTGACGGCCGCCCGCGACTAT
				GCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAACCCACC
				AAGCACCAGTACGACGTGGATACGGAGACGGTCATCGGCTTCCTCCGTGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTC
				GAGCATGAGCTCACCGTGGCCCGCGAGAACGGTTTCCTGGGCTCCATCGGTGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGACACGGACCAGTTCCCTGTGGACCCGTACGATCTTACC
				CAGGCCATGATGCAGGTGCTGCTGAACGGCGGCTTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAACTCCGCCGCTCCTCCACCGACCCTGAGGACATCTTCATCGCCCATATTTCCGCC
				ATGGATGCCATGGCCCACGCTTTGCTTAACGCAGCTGCCGTGCTGGAAGAGAGCCCCCTG
				TGCCAGATGGTCAAGGAGCGTTATGCCAGCTTCGACGGCGGCCTCGGCAAACAGTTCGAG
				GAAGGCAAGGCTACCCTGGAAGACCTGTACGAATACGCCAAGGTCCAGGGTGAACCCGTT
				GTCGCCTCCGGCAAGCAGGAGCTTTACGAGACTCTCCTGAACCTGTATGCCGTCAAGTAA
5753MI3_002	Prevotella	Amino	76	MAKEYFPSIGKIPFEGGDSKNPLAFHYYDAGRVVMGKPMKEWLKFAMAWWHTLGQASGDP
		Acid		FGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYFCFHDVDLIEDCDDIAEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAGTNPQFDVVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLAKLLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIGANR
				GDAQNGWDTDQFPVDPYDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVLEESPLCQMVKERYASFDGGLGKQFEEGKATLEDLYEYAKVQGEPV
				VASGKQELYETLLNLYAVK
1754MI1_001	Prevotella	DNA	77	ATGGCAAAAGAGTATTTTCCGTTTACCGGTAAGATTCCTTTCGAAGGAAAGGACAGTAAG
				AATGTAATGGCTTTCCACTACTACGAGCCTGAGAAGGTCGTGATGGGAAAGAAGATGAAG
				GACTGGCTGAAGTTCGCTATGGCTTGGTGGCATACACTGGGTGGCGCTTCTGCTGACCAG
				TTTGGTGGTCAGACTCGTTCATACGAGTGGGACAAGGCTGGTGACGCTGTTCAGCGCGCT
				AAGGATAAGATGGACGCTGGCTTCGAGATCATGGACAAGCTGGGCATCGAGTACTTCTGC
				TTCCACGATGTTGACCTCGTTGAAGAGGGTGACACCATCGAGGAGTATGAGGCTCGCATG
				AAGGCCATCACCGACTACGCTCAGGAGAAGATGAAGCAGTTCCCCAACATCAAGCTGCTC
				TGGGGTACCGCAAACGTATTCGGTAACAAGCGCTATGCTAACGGTGCTTCTACCAACCCC
				GACTTCGACGTAGTGGCTCGCGCCATCGTTCAGATCAAGAACGCTATTGATGCTACCATC
				AAGCTGGGTGGTACCAACTATGTGTTCTGGGGTGGTCGTGAGGGCTATATGAGTCTGCTG
				AACACCGACCAGAAGCGTGAGAAGGAGCACATGGCTACTATGCTGACCATGGCTCGCGAC
				TATGCTCGCGCCAAGGGATTCAAGGGTACATTCCTCATTGAGCCGAAGCCCATGGAGCCC
				AGCAAGCACCAGTATGATGTGGATACAGAGACCGTTATCGGCTTCCTGAAGGCACACAAC
				CTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCTACACTCGCTGGTCATACC
				TTCGAGCACGAGCTGGCTTGCGCTGTTGACGCTGGTATGCTTGGTTCTATCGACGCTAAC
				CGTGGTGATGCTCAGAACGGTTGGGATACCGACCAGTTCCCCATCGACAACTACGAGCTG
				ACACAGGCTATGCTCGAGATCATCCGCAATGGTGGTCTGGGCAATGGTGGTACCAACTTC
				GATGCTAAGATCCGTCGTAACAGCACCGACCTCGAGGATCTCTTCATCGCTCACATCAGT
				GGTATGGATGCTATGGCACGCGCTCTGATGAACGCTGCTGACATCCTTGAGAACTCTGAG
				CTGCCCGCAATGAAGAAGGCTCGCTACGCAAGCTTCGACCAGGGTGTTGGTAAGGACTTC
				GAAGATGGCAAGCTGACCCTTGAGCAGGTTTACGAGTATGGTAAGAAGGTGGGTGAGCCC
				AAGCAGACTTCTGGTAAGCAGGAGAAGTACGAGACCATCGTTGCTCTCTATGCAAAATAA
1754MI1_001	Prevotella	Amino	78	MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ
		Acid		FGGQTRSYEWDKAGDAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGDTIEEYEARM
				KAITDYAQEKMKQFPNIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATI
				KLGGTNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEP
				SKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDAQNGWDTDQFPIDNYELTQAMLEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALMNAADILENSELPAMKKARYASFDQGVGKDFEDGKLTLEQVYEYGKKVGEP
				KQTSGKQEKYETIVALYAK
1754MI3_007	Prevotella	DNA	79	ATGGCAAAAGAGTATTTTCCGTTTACCGGTAAGATTCCTTTCGAAGGAAAAGAGAGCAAG
				AACGTAATGGCTTTCCATTACTATGAGCCTGAAAAGGTGGTCATGGGCAAGAAAATGAAG
				GATTGGCTGAAATTCGCCATGGCTTGGTGGCACACCCTCGGTGGAGCCAGCGCCGACCAG
				TTCGGTGGACAGACCCGCAGCTATGAGTGGGACAAGGCCGAGGATGCCGTACAGCGTGCT
				AAGGACAAGATGGACGCCGGCTTCGAGATCATGGACAAACTGGGCATCGAGTATTTCTGC
				TTCCACGATGTCGACCTCGTCGACGAGGGTGCTACCGTTGAGGAGTATGAGGCTCGCATG
				AAAGCCATCACCGACTATGCCCAGGTCAAGATGAAGGAATATCCCAACATCAAACTGCTC
				TGGGGCACCGCCAACGTGTTCGGCAACAAGCGTTATGCCAACGGCGCTTCCACCAACCCC
				GACTTCGACGTGGTGGCACGCGCTATCGTTCAGATCAAGAATGCCATCGACGCTACCATC
				AAGCTCGGCGGTCAGAACTACGTGTTCTGGGGCGGACGCGAGGGCTACATGAGCCTGCTC
				AATACCGATCAGAAACGTGAGAAGGAACACATGGCCACCATGCTCACCATGGCGCGCGAC
				TATGCTCGCAGCAAGGGATTCAAGGGCACCTTCCTCATCGAACCCAAACCCATGGAGCCT
				TCCAAGCACCAGTATGATGTCGACACCGAGACGGTCATCGGCTTCCTCCGCGCCCACAAC
				CTCGACAAGGACTTCAAGGTGAACATCGAGGTCAACCACGCCACGCTCGCCGGCCACACC
				TTCGAGCACGAACTGGCTTGCGCCGTCGACGCCGGCATGCTCGGCAGCATCGACGCCAAC
				CGCGGCGACGCACAGAACGGCTGGGATACCGACCAGTTCCCCATCGACAACTACGAACTG
				ACACAGGCCATGCTGGAGATCATCCGCAATGGCGGCCTCGGCAATGGTGGTACCAACTTC
				GACGCCAAGATCCGTCGTAACAGCACCGACCTCGAAGATCTCTTCATCGCTCACATCAGC
				GGTATGGATGCCATGGCTCGCGCGCTGCTCAACGCCGCCGCCATCCTCGAGGAGAGCGAA
				CTGCCCGCCATGAAGAAGGCCCGCTACGCTTCCTTCGACGAAGGTATCGGCAAGGACTTC
				GAAGACGGCAAACTCACCCTCGAGCAGGTTTACGAGTACGGCAAGAAGGTAGGCGAGCCC
				AAGCAGACCTCCGGCAAGCAAGAGAAGTACGAGACCATCGTGGCTCTCTACAGCAAATAA
1754MI3_007	Prevotella	Amino	80	MAKEYFPFTGKIPFEGKESKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ
		Acid		FGGQTRSYEWDKAEDAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVDEGATVEEYEARM
				KAITDYAQVKMKEYPNIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATI
				KLGGQNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEP
				SKHQYDVDTETVIGFLRAHNLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDAQNGWDTDQFPIDNYELTQAMLEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALLNAAAILEESELPAMKKARYASFDEGIGKDFEDGKLTLEQVYEYGKKVGEP
				KQTSGKQEKYETIVALYSK
1754MI5_009	Prevotella	DNA	81	ATGAAAGAGTATTTCCCGCAAATTGGAAAGATTCCCTTCGAGGGACCAGAGAGCAAGAGT
				CCATTGGCGTTCCATTATTATGAGCCGGATCGCATGGTGCTCGGAAAGAGGATGGAGGAT
				TGGCTGAAATTCGCCATGGCATGGTGGCACACCCTTGGCCAGGCCAGCGGCGACCAGTTC
				GGCGGACAGACACGTGAGTACGAGTGGGATAAGGCTGGAGATCCGATACAAAGGGCAAAG
				GATAAGATGGACGCCGGATTCGAGATCATGGAGAAATTGGGTATCAAGTACTTCTGCTTC
				CATGATGTGGATCTCGTCGAGGAAGCTCCCACCATCGCCGAATATGAGGAGCGTATGAGG
				ATCATCACCGACTATGCGCTCGAGAAGATGAAAGCCACTGGCATCAAACTCCTTTGGGGT
				ACAGCCAATGTTTTCGGACATAAGAGATATATGAATGGGGCCGCCACCAACCCGGAGTTC
				GGTGTTGTCGCCAGGGCTGCTGTCCAGATCAAGAACGCGATCGACGCCACCATCAAGCTG
				GGAGGAACAAACTATGTGTTCTGGGGTGGCCGCGAGGGCTACATGAGCCTGCTCAACACC
				CAGATGCAGAGGGAGAAGGACCATCTCGCCAATATGCTCAAGGCTGCTCGTGACTATGCT
				CGCGCCAAGGGATTCAAGGGCACATTCCTCATCGAGCCGAAGCCGATGGAACCTACTAAG
				CATCAGTACGATGTCGACACTGAGACCGTGATCGGCTTCCTCCGCGCAAACGGTCTTGAC
				AAGGATTTCAAGGTCAACATCGAGGTCAATCACGCCACTCTTGCGGGTCACACTTTCGAG
				CATGAGCTCGCCGTGGCTGTCGACAATGGTCTCCTTGGCTCAATCGATGCGAACAGGGGA
				GATTATCAGAACGGTTGGGACACCGACCAGTTCCCTGTTGATCTCTTTGATTTGACCCAG
				GCCATGCTCCAGATCATCCGTAACGGAGGCCTCGGTAATGGTGGATCCAACTTCGACGCC
				AAGCTTCGCCGTAACTCCACTGATCCTGAGGATATATTCATTGCCCATATTTGCGGTATG
				GACGCTATGGCCAGGGCTCTCCTTGCCGCCGCCGCGATCGTGGAGGAGTCTCCTATCCCG
				GCTATGGTCAAAGAGCGTTACGCATCCTTCGACGAAGGTGAGGGCAAGAGATTCGAGGAT
				GGTAAGATGAGTCTGGAGGAACTTGTTGATTACGCGAAGACTCACGGAGAGCCCGCCCAG
				AAGAGTGGCAAACAGGAGCTCTACGAAACCCTTGTCAACATGTACATCAAATAA
1754MI5_009	Prevotella	Amino	82	MKEYFPQIGKIPFEGPESKSPLAFHYYEPDRMVLGKRMEDWLKFAMAWWHTLGQASGDQF
		Acid		GGQTREYEWDKAGDPIQRAKDKMDAGFEIMEKLGIKYFCFHDVDLVEEAPTIAEYEERMR
				IITDYALEKMKATGIKLLWGTANVFGHKRYMNGAATNPEFGVVARAAVQIKNAIDATIKL
				GGTNYVFWGGREGYMSLLNTQMQREKDHLANMLKAARDYARAKGFKGTFLIEPKPMEPTK
				HQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGLLGSIDANRG
				DYQNGWDTDQFPVDLFDLTQAMLQIIRNGGLGNGGSNFDAKLRRNSTDPEDIFIAHICGM
				DAMARALLAAAAIVEESPIPAMVKERYASFDEGEGKRFEDGKMSLEELVDYAKTHGEPAQ
				KSGKQELYETLVNMYIK
5586MI1_003	Prevotella	DNA	83	ATGGCAAAAGAGTATTTTCCGTTTACCGGTAAGATTCCTTTCGAGGGAAAGGACAGTAAG
				AATGTAATGGCGTTCCACTACTACGAGCCCGAGCGCGTGGTAATGGGCAAGAAGATGAAG
				GAGTGGCTGAAGTTTGCCATGGCCTGGTGGCACACGCTGGGTGGAGCCAGTGCCGACCAG
				TTTGGCGGACAGACCCGCAGCTACGAGTGGGACAAGGCTGAAGACGCCGTGCAGCGTGCC
				AAGGACAAGATGGATGCCGGCTTCGAGATCATGGACAAGCTGGGCATCGAGTATTTCTGC
				TTCCATGATGTCGATCTCGTTGACGAGGGTGCCACTGTCGAGGAGTATGAGGCTCGCATG
				CAGGCCATCACCGACTATGCGCAGGAGAAGATGAAGCAGTATCCTGCCATCAAGCTGCTG
				TGGGGTACGGCCAATGTCTTTGGCAACAAGCGTTATGCCAACGGTGCCTCTACCAATCCC
				GACTTCGATGTGGTGGCCCGCGCCATCGTGCAGATTAAGAATGCCATTGATGCCACCATC
				AAGCTGGGCGGCAGCAACTATGTGTTCTGGGGCGGTCGCGAGGGCTACATGTCGCTGCTC
				AACACCGACCAGAAGCGTGAGAAGGAACACATGGCCCGGATGCTGACCATGGCCCGCGAC
				TATGCCCGCTCGAAGGGCTTCAAGGGCAACTTCCTGATTGAGCCCAAGCCCATGGAGCCG
				TCGAAGCATCAGTACGACGTGGACACCGAGACGGTTATCGGATTCCTCCGCGCACATGGC
				CTTGACAAGGACTTCAAGGTGAACATCGAGGTGAACCATGCCACGCTGGCCGGTCATACC
				TTCGAGCACGAACTGGCTTGCGCCGTAGATGCCGGCATGCTGGGCAGCATTGATGCCAAC
				CGCGGCGACGCACAGAACGGATGGGACACCGACCAGTTCCCCATCGACAACTATGAGTTG
				ACACAGGCCATGATGGAGATTATCCGCAATGGCGGTCTGGGTCTTGGCGGTACCAATTTC
				GATGCCAAGATTCGCCGTAACTCCACCGACCTGGAAGACCTCTTCATCGCCCACATCAGT
				GGCATGGACGCCATGGCTCGTGCGCTCCTTAATGCTGCCGACATTCTGGAGAACAGCGAA
				CTGCCCGCCATGAAGAAAGCGCGCTACGCCTCGTTCGACAGTGGCATGGGCAAGGACTTC
				GAGGACGGCAAACTGACCCTTGAGCAGGTTTACGAATACGGCAAAAAAGTCGGCGAACCT
				AAGCAGACCTCCGGCAAGCAGGAGAAGTACGAGACCATCGTGGCTCTCTATGCCAAGTAA
5586MI1_003	Prevotella	Amino	84	MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPERVVMGKKMKEWLKFAMAWWHTLGGASADQ
		Acid		FGGQTRSYEWDKAEDAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVDEGATVEEYEARM
				QAITDYAQEKMKQYPAIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATI
				KLGGSNYVFWGGREGYMSLLNTDQKREKEHMARMLTMARDYARSKGFKGNFLIEPKPMEP
				SKHQYDVDTETVIGFLRAHGLDKDEKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDAQNGWDTDQFPIDNYELTQAMMEIIRNGGLGLGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALLNAADILENSELPAMKKARYASFDSGMGKDFEDGKLTLEQVYEYGKKVGEP
				KQTSGKQEKYETIVALYAK
5586MI2_006	Prevotella	DNA	85	ATGGCAAAAGAGTATTTTCCGTTTACAGGTAAAATTCCTTTCGAAGGAAAGGACAGTAAG
				AACGTAATGGCTTTCCACTACTACGAGCCCGAAAAGGTCGTGATGGGAAAGAAAATGAAA
				GACTGGCTGAAGTTCGCCATGGCCTGGTGGCACACACTGGGTGGCGCCAGCGCCGACCAG
				TTTGGCGGCCAGACACGCAGCTATGAGTGGGACAAGGCTGCCGATGCCGTGCAGCGCGCA
				AAGGACAAGATGGACGCCGGCTTCGAAATCATGGACAAGCTGGGCATCGAGTATTTCTGC
				TTCCACGACGTGGACCTCGTTGAGGAGGGAGCCACCATCGAGGAGTATGAGGCCCGCATG
				AAGGCTATCACCGACTATGCCCAGGAGAAGATGAAACAGTATCCCAGCATCAAGCTGCTC
				TGGGGCACCGCCAATGTGTTTGGCAACAAGCGCTACGCCAACGGCGCCAGCACCAACCCC
				GACTTCGACGTCGTGGCCCGTGCCATCGTGCAGATCAAGAACGCCATCGATGCCACCATC
				AAGCTGGGCGGCACCAACTACGTGTTCTGGGGCGGACGCGAGGGCTACATGAGCCTGCTC
				AACACCGACCAGAAGCGCGAGAAGGAGCACATGGCCACCATGCTCACCATGGCCCGCGAC
				TACGCCCGCGCAAAGGGATTCAAGGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCG
				TCGAAGCACCAGTACGACGTGGACACCGAGACCGTCATCGGTTTCCTGAAGGCCCACGGT
				CTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACGCTGGCCGGCCACACC
				TTCGAGCATGAGCTGGCCTGCGCCGTCGACGCCGGTATGCTGGGCAGCATCGATGCCAAC
				CGCGGCGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCCATCGACAACTTCGAGCTC
				ACCCAGGCCATGATGGAAATTATCCGCAACGGCGGCCTCGGCAACGGCGGCACCAACTTC
				GACGCTAAGATCCGCCGCAACTCCACCGACCTCGAGGACCTCTTCATCGCCCACATCAGC
				GGCATGGACGCCATGGCCCGCGCACTGATGAACGCTGCCGACATTATGGAGAACAGCGAG
				CTGCCCGCCATGAAGAAGGCACGCTACGCCAGCTTCGACGCCGGCATCGGCAAGGACTTT
				GAGGATGGCAAGCTCTCGCTGGAGCAGGTCTACGAGTATGGCAAGAAGGTGGAAGAGCCC
				AAGCAGACCAGCGGCAAGCAGGAGAAGTACGAGACCATCGTCGCCCTCTATGCCAAGTAA
5586MI2_006	Prevotella	Amino	86	MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ
		Acid		FGGQTRSYEWDKAADAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGATIEEYEARM
				KAITDYAQEKMKQYPSIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATI
				KLGGTNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEP
				SKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDAQNGWDTDQFPIDNFELTQAMMEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALMNAADIMENSELPAMKKARYASFDAGIGKDFEDGKLSLEQVYEYGKKVEEP
				KQTSGKQEKYETIVALYAK
5586MI8_003	Prevotella	DNA	87	ATGGCAAAAGAGTATTTCGCCTTTACAGGCAAGATTCCTTTCGAGGGAAAAGACAGTAAG
				AACGTGATGGCTTTCCACTACTACGAGCCGGAGCGTGTGGTGATGGGCAAGAAGATGAAG
				GAGTGGCTGAAGTTCGCCATGGCCTGGTGGCACACACTGGGTGGCGCATCGGCCGACCAG
				TTCGGAGGCCAGACACGCAGCTACGAGTGGGACAAGGCCGCCGACGCCGTGCAGCGCGCC
				AAGGACAAGATGGACGCCGGCTTCGAGATTATGGACAAGCTGGGCATCGAGTACTTCTGC
				TTCCACGATGTAGACCTCGTTGAGGAGGGTGAGACCATAGCCGAGTACGAGCGCCGCATG
				AAGGAAATCACCGACTACGCACAGGAGAAGATGAAGCAGTTCCCCAACATCAAGCTGCTC
				TGGGGCACAGCCAACGTGTTCGGCAACAAGCGCTACGCCAACGGCGCATCGACCAACCCC
				GACTTCGACGTTGTGGCACGCGCCATCGTGCAGATCAAGAACGCCATCGACGCCACCATC
				AAGCTCGGCGGCTCCAACTATGTGTTCTGGGGCGGACGCGAGGGCTATATGAGCCTGCTC
				AACACCGACCAGAAGCGCGAGAAGGAGCACATGGCCACCATGCTCACCATGGCCCGCGAC
				TATGCACGCGCCAAGGGATTCAAGGGCACATTCCTCATCGAGCCGAAGCCCATGGAGCCC
				TCGAAGCACCAGTACGACGTAGACACAGAGACCGTCATCGGCTTCCTCCGTGCACACGGG
				CTGGACAAGGACTTCAAGGTGAACATCGAGGTAAACCACGCCACACTGGCCGGCCACACC
				TTCGAGCACGAGCTGGCTTGCGCCGTCGACGCTGGCATGCTGGGCAGCATCGACGCCAAC
				CGTGGCGACGCACAGAACGGATGGGACACCGACCAGTTCCCCATCGACAACTTCGAGCTC
				ACACAGGCCATGATGGAAATCATCCGCAATGGCGGACTGGGCAATGGCGGCACCAACTTC
				GACGCCAAGATCCGTCGTAACAGCACCGACCTCGAAGACCTCTTCATCGCCCACATCAGC
				GGCATGGACGCCATGGCACGCGCACTGCTCAACGCTGCCGACATCCTGGAGCACAGCGAG
				CTGCCCAAGATGAAGAAGGAGCGCTACGCCAGCTTCGACGCAGGCATCGGCAAGGACTTC
				GAAGACGGCAAGCTCACACTCGAGCAGGTCTACGAGTACGGCAAGAAGGTCGAAGAGCCC
				CGTCAGACCAGCGGCAAGCAGGAGAAGTACGAGACCATCGTCGCCCTCTATGCCAAGTAA
5586MI8_003	Prevotella	Amino	88	MAKEYFAFTGKIPFEGKDSKNVMAFHYYEPERVVMGKKMKEWLKFAMAWWHTLGGASADQ
		Acid		FGGQTRSYEWDKAADAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGETIAEYERRM
				KEITDYAQEKMKQFPNIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATI
				KLGGSNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEP
				SKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDAQNGWDTDQFPIDNFELTQAMMEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALLNAADILEHSELPKMKKERYASFDAGIGKDFEDGKLTLEQVYEYGKKVEEP
				RQTSGKQEKYETIVALYAK
5586MI14_003	Prevotella	DNA	89	ATGGCAAAAGAGTATTTTCCGTTTACTGGTAAGATTCCTTTCGAGGGAAAGGATAGTAAG
				AATGTAATGGCTTTCCACTATTACGAGCCCGAGAAAGTCGTGATGGGAAAGAAGATGAAG
				GACTGGCTGAAGTTCGCAATGGCTTGGTGGCATACACTGGGTGGTGCATCTGCAGACCAG
				TTCGGTGGAGAGACCCGCAGCTACGAGTGGAGCAAGGCTGCTGATCCCGTTCAGCGCGCC
				AAGGACAAGATGGACGCCGGCTTTGAGATTATGGATAAGCTGGGCATCGAGTACTTCTGT
				TTCCACGATATAGACCTCGTTCAGGAGGCAGATACCATTGCAGAATATGAGGAGCGCATG
				AAGGCAATTACCGACTATGCTCTGGAGAAGATGAAGCAGTTCCCCAACATCAAGTTGCTC
				TGGGGTACCGCTAACGTATTTAGCAACAAGCGCTATATGAACGGTGCTTCTACCAATCCC
				GACTTCGACGTGGTGGCCCGTGCCATCGTTCAGATCAAGAACGCTATTGATGCAACCATC
				AAACTCGGTGGTACCAACTATGTATTCTGGGGTGGTCGTGAGGGTTACATGAGCCTATTG
				AATACCGACCAGAAGCGTGAAAAGGAGCACATGGCAATGATGCTCGGTATGGCTCGCGAC
				TATGCCCGCAGCAAGGGATTCAAGGGTACGTTCCTCATCGAGCCGAAGCCGATGGAGCCC
				TCTAAGCATCAGTATGATGTCGATACGGAGACTGTGATTGGTTTCCTGAAGGCACACGGT
				CTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCTACACTGGCTGGTCATACC
				TTCGAGCATGAGCTGGCTTGCGCTGTTGACGCAGGTATGCTGGGCTCTATCGACGCTAAC
				CGCGGTGATGCCCAGAACGGCTGGGATACCGACCAGTTCCCCATCGACAACTACGAGCTG
				ACACAGGCTATGATGGAAATCATCCGCAACGGTGGTCTGGGCAATGGTGGTACCAACTTC
				GACGCTAAGATCCGCCGTAACTCTACCGACCTCGAGGATCTGTTCATCGCTCATATCAGT
				GGTATGGATGCTATGGCCCGTGCTTTGTTGAATGCTGCCGACATTCTGGAGAACTCTGAA
				CTGCCCGCTATGAAGAAGGCCCGCTACGCCAGCTTCGACAACGGTATCGGTAAGGACTTC
				GAGGATGGCAAGCTGACCTTCGAGCAGGTTTACGAATATGGTAAGAAAGTTGAAGAGCCG
				AAGCAGACCTCTGGCAAGCAGGAGAAATACGAGACCATCGTTGCTCTGTATGCTAAATAA
5586MI14_003	Prevotella	Amino	90	MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ
		Acid		FGGETRSYEWSKAADPVQRAKDKMDAGFEIMDKLGIEYFCFHDIDLVQEADTIAEYEERM
				KAITDYALEKMKQFPNIKLLWGTANVFSNKRYMNGASTNPDFDVVARAIVQIKNAIDATI
				KLGGTNYVFWGGREGYMSLLNTDQKREKEHMAMMLGMARDYARSKGFKGTFLIEPKPMEP
				SKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDAQNGWDTDQFPIDNYELTQAMMEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALLNAADILENSELPAMKKARYASFDNGIGKDFEDGKLTFEQVYEYGKKVEEP
				KQTSGKQEKYETIVALYAK
5586MI26_003	Prevotella	DNA	91	ATGGCAAAAGAGTATTTTCCGTTTACCGGTAAAATTCCTTTCGAGGGAAAGGACAGTAAG
				AATGTAATGGCTTTCCACTACTACGAGCCTGAGCGCGTAGTGATGGGAAAGAAGATGAAG
				GATTGGTTGCGATTTGCAATGGCTTGGTGGCACACACTGGGTGGCGCTTCTGCCGACCAG
				TTTGGTGGTCAGACCCGCAGTTACGAATGGGACAAGGCTGCTGATGCTGTTCAGCGTGCT
				AAGGACAAGATGGATGCCGGCTTCGAGATTATGGATAAGCTGGGAATCGAGTTCTTCTGC
				TGGCACGATATCGACCTCGTTGAAGAGGGTGAGACCATTGAAGAGTATGAGCGCCGCATG
				AAGGCTATCACCGACTATGCTCTTGAGAAGATGCAGCAGTATCCCAACATCAAGAACCTC
				TGGGGAACAGCCAATGTGTTTGGCAACAAGCGTTATGCCAACGGTGCCAGCACAAACCCA
				GACTTTGACGTCGTTGCTCGTGCTATCGTACAGATTAAGAATGCTATCGACGCTACTATC
				AAGTTGGGTGGTCAGAATTATGTGTTCTGGGGTGGCCGTGAGGGCTACATGAGCCTGCTC
				AATACTGACCAGAAGCGTGAGAAGGAGCACATGGCTACAATGCTGACCATGGCACGCGAC
				TATGCCCGCAGCAAGGGATTCAAGGGTAACTTCCTCATTGAGCCCAAGCCCATGGAGCCG
				TCAAAGCACCAGTATGATGTTGACACCGAGACCGTATGCGGTTTCCTGCGTGCCCACAAC
				CTTGACAAGGATTTCAAGGTAAATATCGAGGTTAACCATGCTACTCTGGCTGGTCATACT
				TTCGAGCACGAACTGGCATGCGCTGTTGACGCTGGTATGCTTGGTTCTATCGATGCTAAC
				CGTGGTGATGCCCAGAATGGCTGGGATACCGACCAGTTCCCCATCAACAACTATGAACTC
				ACTCAGGCTATGCTTGAGATCATCCGTAATGGTGGTCTGGGTCTTGGCGGCACAAACTTC
				GATGCCAAGATTCGTCGTAACTCAACAGATCTTGAGGATCTCTTCATCGCTCACATCAGT
				GGTATGGATGCCATGGCCCGTGCTCTGCTGAATGCTGCTGCTATTCTGGAGGAGAGCGAG
				CTGCCTAAGATGAAGAAGGAGCGTTATGCTTCTTTCGATGCCGGTATCGGTAAGGACTTC
				GAGGATGGCAAGCTTACCCTTGAGCAGGCTTACGAGTATGGTAAGAAGGTTGAGGAGCCC
				AAGCAGACTTCAGGCAAGCAGGAGAAGTACGAGACCATCGTTGCTCTGTATGCAAAATAA
5586MI26_003	Prevotella	Amino	92	MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPERVVMGKKMKDWLRFAMAWWHTLGGASADQ
		Acid		FGGQTRSYEWDKAADAVQRAKDKMDAGFEIMDKLGIEFFCWHDIDLVEEGETIEEYERRM
				KAITDYALEKMQQYPNIKNLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATI
				KLGGQNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARSKGEKGNFLIEPKPMEP
				SKHQYDVDTETVCGFLRAHNLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDAQNGWDTDQFPINNYELTQAMLEIIRNGGLGLGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALLNAAAILEESELPKMKKERYASFDAGIGKDFEDGKLTLEQAYEYGKKVEEP
				KQTSGKQEKYETIVALYAK
5586MI86_001	Prevotella	DNA	93	ATGAAACAGTATTTTCCCCAGATTGGAAAGATACCCTTCGAGGGTGTAGAGAGCAAGAAT
				GTGATGGCTTTCCACTATTATGAGCCAGAAAGAGTAGTCATGGGCAAGCCTATGAAAGAA
				TGGCTGCGCTTCGCTATGGCGTGGTGGCACACGCTGGGGCAGGCGAGCGGCGACCCCTTC
				GGCGGACAGACCCGCAGCTACGAGTGGGACCGTGCGGCCGACGCGCTACAGCGCGCCAAG
				GACAAGATGGATGCGGGCTTCGAGCTGATGGAGAAGCTTGGCATTGAGTACTTCTGCTTC
				CACGACGTGGACCTCGTAGAAGAGGGCGCCACGGTGGAGGAATACGAGCGGCGGATGGCT
				GCCATCACCGACTACGCGGTAGAGAAGATGCGCGAGCATCCCGAGATACACTGCCTGTGG
				GGCACGGCCAATGTCTTCGGCCACAAGCGCTACATGAACGGAGCCGCCACCAACCCCGAC
				TTCGACGTGGTGGCGCGTGCGGTGGTGCAGATAAAGAACAGCATCGACGCCACGATCAAG
				CTGGGCGGCGAGAACTATGTGTTCTGGGGCGGACGCGAGGGATATATGAGCCTGCTCAAC
				ACCGACCAGCGCCGCGAGAAGGAGCACCTGGCCATGATGCTTGCGAAGGCCCGCGACTAT
				GGCCGCGCCCACGGCTTCAAGGGCACCTTCCTGATAGAGCCCAAGCCGATGGAGCCCATG
				AAGCACCAGTACGACGTGGACACCGAGACGGTGATAGGTTTCCTGCGTGCCCACGGACTG
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACGTTGGCGGGCCACACGTTC
				GAGCACGAGCTGGCCTGTGCCGTCGATGCCGGCATGCTGGGCAGCATCGACGCCAACCGT
				GGCGACGCGCAGAACGGATGGGATACGGACCAGTTCCCCATAGACTGCTACGAGCTCACG
				CAGGCGTGGATGGAGATCATTCGTGGCGGCGGCTTCACCACCGGCGGCACCAACTTCGAC
				GCTAAGCTGCGCCGCAACTCGACCGACCCCGAGGATATCTTCATAGCTCACATCAGCGGC
				ATGGATGCTATGGCCCGCGCCCTGCTCTGCGCCGCCGACATCTTGGAGCACAGCGAGCTG
				CCGGAGATGAAGCGGAAGCGCTATGCCTCGTTCGACAGCGGCATGGGCAAGGAGTTCGAA
				GAGGGCAATCTCAGCTTCGAGCAAATCTATGCCTACGGCAAGCAGGCGGGCGAACCGGCC
				ACGACCAGCGGCAAGCAGGAGAAATACGAAGCCATTGTTTCACTTTATACCCGATGA
5586MI86_001	Prevotella	Amino	94	MKQYFPQIGKIPFEGVESKNVMAFHYYEPERVVMGKPMKEWLRFAMAWWHTLGQASGDPF
		Acid		GGQTRSYEWDRAADALQRAKDKMDAGFELMEKLGIEYFCFHDVDLVEEGATVEEYERRMA
				AITDYAVEKMREHPEIHCLWGTANVFGHKRYMNGAATNPDFDVVARAVVQIKNSIDATIK
				LGGENYVFWGGREGYMSLLNTDQRREKEHLAMMLAKARDYGRAHGFKGTFLIEPKPMEPM
				KHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANR
				GDAQNGWDTDQFPIDCYELTQAWMEIIRGGGFTTGGTNFDAKLRRNSTDPEDIFIAHISG
				MDAMARALLCAADILEHSELPEMKRKRYASFDSGMGKEFEEGNLSFEQIYAYGKQAGEPA
				TTSGKQEKYEAIVSLYTR
5586MI108_002	Prevotella	DNA	95	ATGGCAAAAGAGTATTTTCCGTTTATCGGTAAGGTTCCTTTCGAAGGAACAGAGAGCAAG
				AACGTGATGGCATTCCACTACTATGAGCCCGAAAAGGTGGTCATGGGTAAGAAAATGAAG
				GACTGGCTGAAGTTCGCTATGGCTTGGTGGCACACACTGGGTGGTGCCAGCGCCGACCAG
				TTTGGTGGTCAGACTCGCAGCTACGAGTGGGACAAGGCTGCTGATGCCGTTCAGCGCGCC
				AAGGACAAGATGGATGCTGGCTTCGAGATCATGGATAAGCTCGGCATTGAGTACTTCTGC
				TTCCATGACGTAGACCTCGTTGAGGAGGGTGAAACCGTCGCTGAGTATGAGGCTCGCATG
				AAGGTCATCACCGACTATGCCCTGGAGAAGATGCAGCAGTTCCCCAACATCAAACTGCTC
				TGGGGTACTGCTAACGTGTTCGGCCACAAGCGCTATGCCAACGGTGCCAGCACCAATCCC
				GACTTCGACGTCGTGGCCCGTGCTATCGTTCAGATCAAGAATGCCATCGATGCTACCATT
				AAGCTCGGCGGTACGAACTATGTGTTCTGGGGTGGTCGTGAGGGCTACATGAGCCTTCTC
				AACACCGACCAGAAGCGCGAGAAGGAGCACATGGCAACGATGCTGACCATGGCTCGCGAC
				TATGCCCGCGCCAAGGGATTCAAGGGCACGTTCCTCATCGAGCCGAAGCCCATGGAGCCC
				TCGAAGCATCAGTACGACGTCGACACCGAGACCGTCATCGGCTTCCTCCGTGCCCACGGT
				CTGGATAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACGCTGGCCGGTCATACC
				TTCGAGCACGAACTGGCTTGCGCCGTTGATGCCGGCATGCTCGGCTCTATCGATGCCAAC
				CGCGGCGACGCTCAGAACGGCTGGGACACCGACCAGTTCCCCATCGACAACTACGAGCTC
				ACTCAGGCCATGATGGAAATCATCCGTAATGGCGGTCTGGGCAACGGCGGCACGAACTTC
				GATGCCAAGATCCGTCGTAACAGCACCGACCTCGAGGACCTCTTCATCGCTCACATCAGC
				GGCATGGATGCCATGGCACGCGCTCTGATGAACGCTGCTGCCATCCTCGAAGAGAGCGAG
				CTGCCCGCCATGAAGAAGGCCCGCTATGCTTCGTTCGACGAGGGTATCGGCAAGGACTTC
				GAGGACGGCAAGTTGTCACTTGAGCAGGTCTACGAATATGGTAAGAAGGTTGAGGAGCCC
				AAGCAGACCTCGGGCAAGCAGGAGAAGTACGAGACCATCGTGGCCCTCTATGCCAAGTAA
5586MI108_002	Prevotella	Amino	96	MAKEYFPFIGKVPFEGTESKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ
		Acid		FGGQTRSYEWDKAADAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGETVAEYEARM
				KVITDYALEKMQQFPNIKLLWGTANVFGHKRYANGASTNPDFDVVARAIVQIKNAIDATI
				KLGGTNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEP
				SKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDAN
				RGDAQNGWDTDQFPIDNYELTQAMMEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALMNAAAILEESELPAMKKARYASFDEGIGKDFEDGKLSLEQVYEYGKKVEEP
				KQTSGKQEKYETIVALYAK
5586MI182_004	Prevotella	DNA	97	ATGGCAAAAGAGTATTTTCCGTTTGTTGGTAAGATTCCTTTCGAGGGAAAGGATAGTAAG
				AATGTAATGGCTTTCCACTATTACGAACCAGAGAAGGTCGTGATGGGAAAGAAGATGAAG
				GACTGGCTGAAGTTCGCCATGGCATGGTGGCACACACTGGGACAGGCCAGTGCCGACCCG
				TTTGGAGGTCAGACCCGCAGCTACGAGTGGGACAAGGCTGACGATGCTGTGCAGCGCGCA
				AAGGACAAGATGGATGCCGGATTTGAGATCATGGACAAGCTGGGCATCGAGTACTTCTGC
				TTCCACGATGTAGACCTCGTTGAGGAGGGAGCAACTGTTGAGGAGTACGAGGCTCGCATG
				AAGGCCATCACCGACTATGCATTGGAGAAGATGAAAGAGTATCCCAACATCAAGAACCTC
				TGGGGTACAGCCAATGTATTCAGCAACAAGCGCTATATGAACGGTGCCAGCACCAACCCC
				GACTTCGACGTTGTTGCACGTGCCATCGTACAGATAAAGAACGCCATTGACGCTACCATC
				AAGCTCGGCGGTCAGAACTACGTGTTCTGGGGCGGACGTGAGGGATACATGAGCCTGCTC
				AACACCGACCAGAAGCGCGAGAAGGAGCACATGGCAACCATGCTGACCATGGCTCGCGAC
				TACGCTCGCAAGAACGGTTTCAAGGGCACATTCCTCATCGAGCCTAAGCCCATGGAACCC
				TCAAAGCACCAGTACGACGTAGACACAGAGACCGTATGCGGTTTCCTCCGCGCCCATGGT
				CTTGACAAGGATTTCAAGGTGAACATTGAGGTGAACCACGCTACCCTCGCCGGCCACACC
				TTTGAGCATGAACTGGCTTGCGCCGTCGACAACGGCATGCTCGGCAGCATCGATGCCAAC
				CGCGGCGACGTTCAGAACGGCTGGGACACCGACCAGTTCCCCATCGACAACTACGAGCTG
				ACTCAGGCCATGCTCGAAATCATCCGCAACGGTGGTCTGGGCAACGGCGGTACCAACTTC
				GACGCCAAGATCCGTCGTAACTCTACCGACCTCGAGGATCTGTTCATCGCCCACATCAGC
				GGTATGGACGCCATGGCACGTGCACTGCTCAATGCAGCAGCCATACTGGAGGAGAGCGAG
				CTGCCTGCCATGAAGAAGGAGCGTTACGCCAGCTTCGACAGCGGCATCGGCAAGGACTTC
				GAGGACGGCAAGCTCACACTTGAGCAGGCCTATGAGTATGGTAAGAAGGTTGAGGAGCCA
				AAGCAGACCTCTGGCAAGCAGGAGAAGTATGAGACTATAGTAGCCCTCTACGCTAAGTAG
5586MI182_004	Prevotella	Amino	98	MAKEYFPFVGKIPFEGKDSKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKADDAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGATVEEYEARM
				KAITDYALEKMKEYPNIKNLWGTANVFSNKRYMNGASTNPDFDVVARAIVQIKNAIDATI
				KLGGQNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARKNGFKGTFLIEPKPMEP
				SKHQYDVDTETVCGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDNGMLGSIDAN
				RGDVQNGWDTDQFPIDNYELTQAMLEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHIS
				GMDAMARALLNAAAILEESELPAMKKERYASFDSGIGKDFEDGKLTLEQAYEYGKKVEEP
				KQTSGKQEKYETIVALYAK
5586MI193_004	Prevotella	DNA	99	ATGACTAAAGAGTATTTCCCTACCATTGGCAAGATTCCCTTTGAGGGACCTGAAAGCAAG
				AACCCGCTTGCATTCCATTACTATGAGCCCGACCGCCTGGTCATGGGCAAGAAGATGAAA
				GACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCCGGCGACCAG
				TTCGGCGGCCAGACCCGCCACTATGCCTGGGATGATCCGGATTGCCCGTATGCACGTGCC
				AAAGCCAAGGCCGACGCCGGTTTCGAAATCATGCAGAAACTGGGCATTGAATTCTTCTGC
				TTCCACGACATCGACCTGGTCGAGGATGCCGATGAAATCGCCGAGTACGAGGCCCGGATG
				AAGGACATCACCGACTATCTGCTCGTCAAGATGAAAGAGACCGGCATCAAGAACCTTTGG
				GGAACGGCCAACGTATTTGGCCACAAGCGCTACATGAACGGCGCCGCCACCAACCCCGAT
				TTCGACGTGCTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAG
				TTGGGCGGTCAGAACTATGTGTTCTGGGGCGGCCGTGAAGGCTACCAGACCCTGCTCAAT
				ACCCAGATGCAGCGCGAGAAGGAACACATGGGCCGTATGTTGGCACTGGCCCGCGACTAT
				GGCCGTGCACACGGTTTCAAGGGCACGTTCCTCATCGAGCCCAAACCGATGGAGCCGACC
				AAGCACCAGTACGATCAGGATACGGAAACCGTCATCGGCTTCCTGCGCCGCCATGGCCTC
				GACAAGGACTTCAAGGTCAACATCGAGGTGAACCATGCTACCCTGGCGGGCCACACCTTC
				GAGCACGAGCTGGCTTGCGCCGTCGACCACGGCATGCTGGGCAGCATCGACGCCAACCGG
				GGTGATGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGATAACTATGAGCTGACG
				CTGGCCATGCTCCAGATCATCCGCAACGGCGGCCTGGCACCCGGCGGCTCGAACTTCGAT
				GCGAAGCTGCGTCGCAACTCCACCGATCCGGAAGATATCTTCATCGCGCACATCAGCGCC
				ATGGATGCCATGGCCCGCGCCCTGGTCAATGCTGTCGCCATTCTCGAGGAATCGCCCATC
				CCGGCCATGGTCAGGGAACGTTACGCCTCGTTCGACAGCGGAAAGGGCAGGGAATATGAG
				GAAGGCAGGCTGTCTCTCGAAGACATCGTGGCCTATGCCAAAGCCCACGGCGAACCGAAA
				CAGATTTCCGGCAAGCAGGAACTCTACGAAACCATCGTGGCTCTCTATTGCAAGTAG
5586MI193_004	Prevotella	Amino	100	MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRLVMGKKMKDWLRFAMAWWHTLGQASGDQ
		Acid		FGGQTRHYAWDDPDCPYARAKAKADAGFEIMQKLGIEFFCFHDIDLVEDADEIAEYEARM
				KDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVLARAAVQIKNAIDATIK
				LGGQNYVFWGGREGYQTLLNTQMQREKEHMGRMLALARDYGRAHGFKGTFLIEPKPMEPT
				KHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANR
				GDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALVNAVAILEESPIPAMVRERYASFDSGKGREYEEGRLSLEDIVAYAKAHGEPK
				QISGKQELYETIVALYCK
5586MI195_003	Prevotella	DNA	101	ATGGCAAAAGAGTATTTCCCGCAGATCGGAAAGATCGGCTTTGAGGGTCCTGCAAGCAAG
				AACCCGCTGGCATTCCATTATTATGACGCCGAGCGCGTGGTGATGGGTAAACCCATGAAA
				GACTGGTTTAAATTCGCCCTCGCGTGGTGGCACAGCCTCGGCCAGGCCTCCGGCGACCCG
				TTCGGCGGCCAGACCCGCTCCTACGAGTGGGACAAGGGCGAATGCCCCTACTGCCGCGCC
				CGCGCCAAGGCGGACGCCGGCTTCGAGATCATGCAAAAGCTCGGCATCGGCTATTTCTGC
				TTCCACGACGTCGACCTCATCGAAGACACGGACGACATCGCCGAATATGAGGCCCGCCTC
				AAGGACATCACGGACTACCTGCTCGAAAGGATGCAGGAAACCGGCATCAAGAACCTCTGG
				GGCACGGCCAATGTCTTCGGTCACAAGCGCTACATGAACGGCGCCGGCACCAATCCGCAG
				TTCGACATCGTCGCCCGCGCTGCCGTCCAGATCAAGAACGCCCTCGACGCCACCATCAAG
				CTCGGTGGCTCGAACTACGTCTTCTGGGGCGGCCGCGAAGGTTATTACACGCTGCTCAAC
				ACCCAGATGCAGCGCGAGAAAGACCACCTCGCCAAGCTCCTCACCGCCGCCCGCGACTAT
				GCCCGCGCCAAGGGCTTCCAGGGCACCTTCCTGATCGAGCCCAAGCCGATGGAGCCGACC
				AAGCACCAGTACGATGTCGACACGGAGACTGTAATCGGATTCCTCCGCGCCAACGGACTG
				GACAAGGACTTCAAGGTCAACATCGAGGTCAACCACGCCACCCTCGCCGGCCATACCTTC
				GAGCATGAGCTGACCGTCGCCCGCGAGAACGGATTCCTCGGCAGCATCGACGCCAACCGC
				GGTGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCCGTGGACGCCTACGACCTCACC
				CAGGCCATGATGCAGGTGCTCCTGAACGGCGGTTTCGGCAACGGCGGCACCAATTTCGAC
				GCCAAGCTCCGTCGCAGCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCGGCCATTCTCGAGGAGAGCCCGCTG
				CCCGCGATGGTCAAGGAGCGTTACGCCTCCTTCGACAGCGGTCTCGGCAAGCAGTTCGAG
				GAGGGAAAGGCCACGCTGGAGGACCTCTACGACTACGCCAAGGCCCATGGCGAGCCCGTC
				GCCGCCTCCGGCAAGCAGGAACTGTGTGAAACTTACCTGAATCTGTATGCAAAGTAA
5586MI195_003	Prevotella	Amino	102	MAKEYFPQIGKIGFEGPASKNPLAFHYYDAERVVMGKPMKDWFKFALAWWHSLGQASGDP
		Acid		FGGQTRSYEWDKGECPYCRARAKADAGFEIMQKLGIGYFCFHDVDLIEDTDDIAEYEARL
				KDITDYLLERMQETGIKNLWGTANVFGHKRYMNGAGTNPQFDIVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLAKLLTAARDYARAKGFQGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDAYDLTQAMMQVLLNGGEGNGGTNEDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPLPAMVKERYASFDSGLGKQFEEGKATLEDLYDYAKAHGEPV
				AASGKQELCETYLNLYAK
5586MI196_003	Prevotella	DNA	103	ATGACAAAAGAGTATTTCCCTACCATCGGCAAGATCCCCTTTGAGGGACCCGAGAGCAAA
				AACCCCCTCGCTTTTCATTACTATGAGCCCGACCGCCTGGTCATGGGCAAGAAGATGAAA
				GACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCCGGCGACCAG
				TTTGGCGGCCAGACCCGCCACTATGCCTGGGATGATCCGGATTGCCCGTATGCACGTGCC
				AAAGCCAAGGCCGACGCCGGTTTCGAAATCATGCAGAAACTGGGCATTGAATTCTTCTGC
				TTCCACGACATCGACCTGATCGAGGATACCGATGACATCGTCGAGTATGAGGCCCGGATG
				AAGGACATCACCGACTATCTGCTGGTCAAGATGAAAGAGACCGGCATCAAGAATCTCTGG
				GGAACGGCCAACGTATTCGGGCACAAGCGCTATATGAACGGCGCTGCCACCAACCCCGAT
				TTCGACGTGCTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTGGGCGGCCAGAATTATGTGTTCTGGGGCGGGCGTGAAGGCTACCAGAGCCTGCTCAAT
				ACCCAGATGCAGCGCGAAAAGGAACACATGGGCCGTATGTTGGCACTAGCCCGCGACTAT
				GGCCGTGCACACGGTTTCAAGGGCACGTTCCTCATCGAGCCCAAACCGATGGAGCCGACC
				AAGCACCAGTACGATCAGGATACGGAGACCGTCATCGGTTTTCTGCGCCGCCATGGCCTC
				GACAAGGACTTCAAGGTCAACATCGAGGTGAACCATGCTACCCTGGCGGGCCACACCTTC
				GAGCACGAGCTGGCCTGCGCCGTCGACCACGGCATGCTGGGCAGTATTGACGCCAACCGC
				GGTGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGATAACTATGAGCTGACG
				CTGGCCATGCTCCAGATCATCCGCAACGGCGGCCTGGCACCCGGCGGCTCGAACTTCGAT
				GCGAAGCTGCGTCGCAACTCCACCGATCCGGAAGATATCTTCATCGCGCACATCAGCGCC
				ATGGATGCCATGGCCCGCGCCCTGGTCAACGCTGTCGCCATTCTTGAGGAATCGCCCATT
				CCGGACATGGTCAAGGAGCGCTACGCTTCGTTCGACAGCGGAAAAGGCAGGGAGTACGAA
				GAGGGGAAACTTTCCTTCGAGGACCTCGTGGCCTATGCCAAAGCCCACGGCGAACCGAAA
				CAGATTTCCGGCAAGCAGGAACTCTACGAAACCATCGTGGCTCTCTATTGCAAGTAG
5586MI196_003	Prevotella	Amino	104	MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRLVMGKKMKDWLRFAMAWWHTLGQASGDQ
		Acid		FGGQTRHYAWDDPDCPYARAKAKADAGFEIMQKLGIEFFCFHDIDLIEDTDDIVEYEARM
				KDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVLARAAVQIKNAIDATIK
				LGGQNYVFWGGREGYQSLLNTQMQREKEHMGRMLALARDYGRAHGFKGTFLIEPKPMEPT
				KHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANR
				GDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALVNAVAILEESPIPDMVKERYASFDSGKGREYEEGKLSFEDLVAYAKAHGEPK
				QISGKQELYETIVALYCK
5586MI197_003	Prevotella	DNA	105	ATGACAAAAGAGTATTTCCCTACCATCGGCAAGATCCCCTTTGAGGGACCCGAGAGCAAA
				AACCCCCTCGCTTTTCATTACTATGAGCCCGACCGCCTGGTCATGGGCAAGAAGATGAAA
				GACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCCGGCGACCAG
				TTTGGCGGCCAGACCCGCCACTATGCCTGGGATGATCCGGATTGCCCGTATGCACGTGCC
				AAAGCCAAGGCCGACGCCGGTTTCGAAATCATGCAGAAACTGGGCATTGAATTCTTCTGC
				TTCCACGACATCGACCTGATCGAGGATACCGATGACATCGTCGAGTATGAGGCCCGGATG
				AAGGACATCACCGACTATCTGCTGGTCAAGATGAAAGAGACCGGCATCAAGAATCTCTGG
				GGAACGGCCAACGTATTCGGGCACAAGCGCTATATGAACGGCGCTGCCACCAACCCCGAT
				TTCGACGTGCTGGCCCGTGCCGCCGCCCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTGGGCGGCCAGAATTATGTGTTCTGGGGCGGGCGTGAAGGCTACCAGAGCCTGCTCAAT
				ACCCAGATGCAGCGCGAAAAGGAACACATGGGCCGTATGTTGGCACTAGCCCGCGACTAT
				GGCCGTGCACACGGTTTCAAGGGCACGCTCCTCATCGAGCCCAAACCGATGGAGCCGACC
				AAGCACCAGTACGATCAGGATACGGAGACCGTCATCGGTTTTCTGCGCCGCCATGGCCTC
				GACAAGGACTTCAAGGTCAACATCGAGGTGAACCATGCTACCCTGGCGGGCCACACCTTC
				GAGCACGAGCTGGCCTGCGCCGTCGACCACGGCATGCTGGGCAGTATTGACGCCAACCGC
				GGTGACGCCCAGGACGGCTGGGACACCGACCAGTTCCCGATCGATAACTATGAGCTGACG
				CTGGCCATGCTCCAGATCATCCGCAACGGCGGCCTGGCACCCGGCGGCTCGAACTTCGAT
				GCGAAGCTGCGTCGCAACTCCACCGATCCGGAAGATATCTTCATCGCGCACATCAGCGCC
				ATGGATGCCATGGCCCGCGCCCTGGTCAACGCTGTCGCCATTCTTGAGGAATCGCCCATT
				CCGGACATGGTCAAGGAGCGCTACGCTTCGTTCGACAGCGGAAAAGGCAGGGAGTACGAA
				GAGGGGAAACTTTCCTTCGAGGACCTCGTGGCCTATGCCAAAGCCCACGGCGAACCGAAA
				CAGATTTCCGGCAAGCAGGAACTCTACGAAACCATCGTGGCTCTCTATTGCAAGTAG
5586MI197_003	Prevotella	Amino	106	MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRLVMGKKMKDWLRFAMAWWHTLGQASGDQ
		Acid		FGGQTRHYAWDDPDCPYARAKAKADAGFEIMQKLGIEFFCFHDIDLIEDTDDIVEYEARM
				KDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVLARAAAQIKNAIDATIK
				LGGQNYVFWGGREGYQSLLNTQMQREKEHMGRMLALARDYGRAHGFKGTLLIEPKPMEPT
				KHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANR
				GDAQDGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALVNAVAILEESPIPDMVKERYASFDSGKGREYEEGKLSFEDLVAYAKAHGEPK
				QISGKQELYETIVALYCK
5586MI199_003	Prevotella	DNA	107	ATGACAAAAGAGTATTTCCCTACCATCGGCAAGATCCCCTTTGAGGGACCCGAGAGCAAA
				AACCCCCTCGCTTTTCATTACTATGAGCCCGACCGCCTGGTCATGGGCAAGAAGATGAAA
				GACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCCGGCGACCAG
				TTTGGCGGCCAGACCCGCCACTATGCCTGGGATGATCCGGATTGCCCGTATGCACGTGCC
				AAAGCCAAGGCCGACGCCGGTTTCGAAATCATGCAGAAACTGGGCATTGAATTCTTCTGC
				TTCCACGACATCGACCTGATCGAGGATACCGATGACATCGTCGAGTATGAGGCCCGGATG
				AAGGACATCACCGACTATCTGCTGGTCAAGATGAAAGAGACCGGCATCAAGAATCTCTGG
				GGAACGGCCAACGTATTCGGGCACAAGCGCTATATGAACGGCGCTGCCACCAACCCCGAT
				TTCGACGTGCTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTGGGCGGCCAGAATTATGTGTTCTGGGGCGGGCGTGAAGGCTACCAGAGCCTGCTCAAT
				ACCCAGATGCAGCGCGAAAAGGAACACATGGGCCGTATGTTGGCACTAGCCCGCGACTAT
				GGCCGTGCACACGGTTTCAAGGGCACGTTCCTCATCGAGCCCAAACCGATGGAGCCGACC
				AAGCACCAGTACGATCAGGATACGGAGACCGTCATCGGTTTTCTGCGCCGCCATGGCCTC
				GACAAGGACTTCAAGGTCAACATCGAGGTGAACCATGCTACCCTGGCGGGCCACACCTTC
				GAGCACGAGCTGGCCTGCGCCGTCGACCACGGCATGCTGGGCAGTATTGACGCCAACCGC
				GGTGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGATAACTATGAGCTGACG
				CTGGCCATGCTCCAGATCATCCGCAACGGCGGCCTGGCACCCGGCGGCTCGAACTTCGAT
				GCGAAGCTGCGTCGCAACTCCACCGATCCGGAAGATGTCTTCATCGCGCACATCAGCGCC
				ATGGATGCCATGGCCCGCGCCCTGGTCAACGCTGTCGCCATTCTTGAGGAATCGCCCATT
				CCGGACATGGTCAAGGAGCGCTACGCTTCGTTCGACAGCGGAAAAGGCAGGGAGTACGAA
				GAGGGGAAACTTTCCTTCGAGGACCTCGTGGCCTATGCCAAAGCCCACGGCGAACCGAAA
				CAGATTTCCGGCAAGCAGGAACTCTACGAAACCATCGTGGCTCTCTATTGCAAGTAG
5586MI199_003	Prevotella	Amino	108	MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRLVMGKKMKDWLRFAMAWWHTLGQASGDQ
		Acid		FGGQTRHYAWDDPDCPYARAKAKADAGFEIMQKLGIEFFCFHDIDLIEDTDDIVEYEARM
				KDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVLARAAVQIKNAIDATIK
				LGGQNYVFWGGREGYQSLLNTQMQREKEHMGRMLALARDYGRAHGFKGTFLIEPKPMEPT
				KHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANR
				GDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDVFIAHISA
				MDAMARALVNAVAILEESPIPDMVKERYASFDSGKGREYEEGKLSFEDLVAYAKAHGEPK
				QISGKQELYETIVALYCK
5586MI200_003	Prevotella	DNA	109	ATGGCAAAAGAGTATTTCCCGACAATCGGAAAGATCCCCTTCGAGGGCGTTGAGAGCAAG
				AATCCCCTTGCTTTCCATTATTATGACGCCGAGCGCGTGGTCATGGGCAAGCCCATGAAG
				GACTGGTTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGCCAGGCTTCCGCGGACCCG
				TTCGGCGGCCAGACCCGCTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCC
				CGCGCCAAGGCTGACGCCGGCTTCGAGATCATGCAGAAGCTCGGAATCGGCTACTATTGC
				TTCCACGACATCGACCTGGTGGAGGACACCGAGGACATCGCCGAATACGAGGCCCGCATG
				AAGGACATCACCGACTACCTCGTCGAGAAGCAGAAGGAGACCGGCATCAAGAACCTCTGG
				GGCACCGCGAACGTGTTCGGCAACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAG
				TTCGACATCGTCGCCCGCGCGGCCCTGCAGATCAAGAACGCGATCGATGCCACCATCAAG
				CTCGGCGGCACCGGCTACGTGTTCTGGGGCGGCCGGGAAGGCTACTACACCCTGCTGAAC
				ACCCAGATGCAGCGCGAGAAGGACCACCTCGCCAAGATGCTCACCGCCGCCCGCGACTAC
				GCCCGCGCCAACGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCGATGGAGCCCACC
				AAGCACCAATACGACGTGGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAATGGCCTG
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTCGCCGGCCACACCTTC
				GAGCACGAGCTCACCGTGGCCGTTGACAACGGCTTCCTCGGCAGCATCGACGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGGTGGATCCGTACGATCTCACC
				CAGGCGATGATCCAGATCATCCGCAACGGCGGCTTCAAGGACGGCGGCACCAACTTCGAC
				GCCAGGCTCCGCCGCTCTTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTCATCGAGGAGAGCCCGCTC
				TGCGAGATGGTCGCCAAGCGTTACGCTTCCTTCGACAGCGGCCTCGGCAAAAAGTTCGAG
				GAAGGCAAGGCCACCCTCGAGGAACTCTACGAGTATGCCAAGGCGAACGGTGAGGTCAAG
				GCCGAATCCGGCAAGCAGGAGCTCTACGAGACCCTTCTGAACCTCTACGCGAAATAG
5586MI200_003	Prevotella	Amino	110	MAKEYFPTIGKIPFEGVESKNPLAFHYYDAERVVMGKPMKDWFKFAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKGECPYCRARAKADAGFEIMQKLGIGYYCFHDIDLVEDTEDIAEYEARM
				KDITDYLVEKQKETGIKNLWGTANVFGNKRYMNGAATNPQFDIVARAALQIKNAIDATIK
				LGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARANGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANR
				GDAQNGWDTDQFPVDPYDLTQAMIQIIRNGGFKDGGTNFDARLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVIEESPLCEMVAKRYASFDSGLGKKFEEGKATLEELYEYAKANGEVK
				AESGKQELYETLLNLYAK
5586MI203_003	Prevotella	DNA	111	ATGGCACAAGCGTATTTTCCTACCATCGGGAAAATCCCCTTCGAGGGACCCGAAAGCAAG
				AATCCCCTGGCATTCCATTATTATGAGCCCGACCGCCTGGTCCTGGGCAAGAAGATGAAG
				GACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACGCTGGGCCAGGCTTCCGGCGACCAG
				TTCGGCGGCCAGACCCGCCACTACGCCTGGGACGAGCCCGCCACGCCCCTGGAACGGGCC
				AAGGCCAAGGCGGATGCCGGTTTCGAGATCATGCAGAAACTGGGCATCGAATTCTTCTGC
				TTCCACGATGTGGACCTCATCGAAGAGGGCGCCACGATCGAGGAATACGAGCAGCGGATG
				CAGCAGATCACGGATTATCTGCTGGTCAAGATGAAAGAGACCGGCATCCGCAACCTCTGG
				GGTACGGCCAACGTGTTCGGACACGAGCGCTACATGAACGGCGCGGCCACGAACCCCGAT
				TTCGATGTCGTGGCCCGCGCGGCCGTGCAGATCAAGACGGCCATCGACGCCACCATCAAG
				TTGGGCGGCGAGAACTATGTGTTCTGGGGCGGCCGGGAAGGCTATATGAGCCTGCTCAAT
				ACGCAGATGCACCGCGAGAAGCTGCATCTGGGCAAGATGCTCGCCGCGGCCCGCGACTAC
				GGACGCGCCCACGGCTTCAAGGGGACCTTCCTCATCGAACCCAAGCCGATGGAACCCACC
				AAGCATCAGTATGACCAGGATACGGAGACGGTCATCGGTTTCCTGCGCCGCTACGGCCTG
				GACGAAGACTTCAAGGTGAACATCGAGGTCAACCACGCTACGCTGGCCGGCCATACCTTC
				GAACACGAACTGGCCACGGCGGTCGATGCCGGCCTGCTGGGCAGCATCGACGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGATCGACAACTACGAACTGACC
				CTGGCGATGCTGCAGGTCATCCGCAACGGCGGTCTGGCCCCGGGCGGCTCGAATTTCGAT
				GCCAAGCTCCGCCGGAACTCCACCGATCCGGAAGACATCTTCATTGCCCACATCAGCGCG
				ATGGATGCGATGGCGCGGGCCCTGCTCAATGCGGCCGCCCTCTGCGAGACGTCCCCGATT
				CCGGCGATGGTCAAGGCGCGTTACGCTTCGTTCGACAGCGGCGCCGGCAAGGATTTCGAA
				GAGGGAAGGATGACGCTGGAAGACCTCGTGGCCTATGCCAGGACCCACGGCGAGCCGAAG
				CGGACCTCGGGCAAGCAGGAACTCTATGAGACCCTCGTGGCGCTTTATTGCAAATAG
5586MI203_003	Prevotella	Amino	112	MAQAYFPTIGKIPFEGPESKNPLAFHYYEPDRLVLGKKMKDWLRFAMAWWHTLGQASGDQ
		Acid		FGGQTRHYAWDEPATPLERAKAKADAGFEIMQKLGIEFFCFHDVDLIEEGATIEEYEQRM
				QQITDYLLVKMKETGIRNLWGTANVFGHERYMNGAATNPDFDVVARAAVQIKTAIDATIK
				LGGENYVFWGGREGYMSLLNTQMHREKLHLGKMLAAARDYGRAHGFKGTFLIEPKPMEPT
				KHQYDQDTETVIGFLRRYGLDEDFKVNIEVNHATLAGHTFEHELATAVDAGLLGSIDANA
				GDAQNGWDTDQFPIDNYELTLAMLQVIRKGGLAPGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALLNAAALCETSPIPAMVKARYASFDSGAGKDFEEGRMTLEDLVAYARTHGEPK
				RTSGKQELYETLVALYCK
5586MI205_004	Prevotella	DNA	113	ATGACCAACGAGTATTTTCCCGGAATCGGTGTGATTCCGTTTGAAGGACAGGAAAGCAAG
				AATCCCCTGGCTTTCCATTATTATGACGCCAACCGCGTAGTGATGGGCAAACCCATGAAG
				GAATGGTTCAAATTTGCCATGGCCTGGTGGCATACGCTGGGGCAGGCATCGGCCGATCCC
				TTCGGCGGACAGACCCGCTCCTACGCATGGGACAAGGGCGAGTGCCCTTACTGCCGTGCC
				CGCCAGAAGGCCGACGCCGGCTTTGAACTGATGCAGAAGCTGGGAATCGGCTATTTCTGC
				TTCCACGATGTGAATATCATCGAGGACTGCGAGGACATTGCCGAGTATGAGGCCCGTATG
				AAGGACATCACGGACTATCTGCTGGTGAAGATGAAGGAAACGGGCATCAAGAATCTGTGG
				GGCACGGCCAACGTCTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAACCCGCAA
				TTCGACGTGGTAGCCCGCGCTGCGGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAG
				CTGGGCGGCAGCAATTATGTGTTCTGGGGCGGCCGGGAAGGCTACTACACCCTTTTGAAC
				ACGCAGATGCAGCGGGAGAAGGACCACCTGGCCCAGATGCTCAAGGCGGCCCGCGACTAT
				GCCCGCGGCAAGGGATTCAAGGGCACGTTCCTCATTGAGCCCAAGCCCATGGAGCCCACC
				AAGCACCAGTACGACGTAGATACGGAGACCGTGATTGGTTTCCTGCGCGCCAACGGGCTG
				GACAAGGACTTCAAGGTGAATATCGAAGTGAACCACGCCACCCTGGCCGGCCATACCTTC
				GAGCACGAGCTCACCGTGGCCCGCGAAAACGGCTTCCTGGGCAGCATCGACGCCAACCGC
				GGAGACGCCCAGAACGGCTGGGATACAGACCAGTTCCCCGTGGACGCCTTTGACCTCACC
				CAGGCCATGATGCAGGTCCTGCTCAACGGCGGATTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAACTGCGCCGTTCCTCCACGGATCCCGAGGACATCTTCATCGCCCACATCAGCGCC
				ATGGACGCCATGGCCCACGCCCTCCTGAACGCCGCCGCCATCCTGGAAGAGAGCCCCATG
				CCGGGCATGGTGAAGGAGCGCTACGCTTCCTTCGACAATGGCCTTGGCAAGAAGTTCGAG
				GAAGGAAAGGCCACGCTGGAAGAGCTGTACGACTATGCCAAGAAGAACGGCGAGCCTGTG
				GCCGCTTCCGGAAAGCAGGAACTGTACGAAACGCTGCTGAACCTGTACGCCAAGTAA
5586MI205_004	Prevotella	Amino	114	MTNEYFPGIGVIPFEGQESKNPLAFHYYDANRVVMGKPMKEWFKFAMAWWHTLGQASADP
		Acid		FGGQTRSYAWDKGECPYCRARQKADAGFELMQKLGIGYFCFHDVNIIEDCEDIAEYEARM
				KDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDVVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLAQMLKAARDYARGKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDEKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDAFDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPMPGMVKERYASFDNGLGKKFEEGKATLEELYDYAKKNGEPV
				AASGKQELYETLLNLYAK
5586MI206_004	Prevotella	DNA	115	ATGGCAAAAGAGTATTTCCCGACTATCGGCAAGATTCCCTTCGAGGGCGTCGAATCCAAG
				AACCCGATGGCATTCCACTATTATGACGCGAAACGCGTCGTGATGGGCAAGCCCATGAAG
				GACTGGCTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGACAGGCTTCCGGCGACCCG
				TTCGGCGGCCAGACCCGTTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCC
				AAGGCCAAGGCCGACGCCGGTTTCGAGATCATGCAGAAACTGGGCATCGAGTACTACTGC
				TTCCATGACATCGACCTGGTGGAGGACACCGAGGACATCGCCGAGTACGAGGCCCGCATG
				AAGGACATCACCGACTACCTCGTCGAGAAGCAGAAGGAGACCGGTATCAAGAACCTCTGG
				GGCACGGCCAACGTGTTCGGCAACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAG
				TTCGACGTCGTCGCCCGCGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAA
				CTCGGCGGCACCTCTTACGTGTTCTGGGGCGGCCGTGAAGGCTACTACACCCTCCTGAAC
				ACCCAGATGCAGCGCGAGAAGGACCACCTCGCCAAGATGCTCACCGCCGCCCGCGACTAC
				GCCCGCGCCCACGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCCACC
				AAGCACCAGTACGACGTGGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTCAATATCGAAGTGAACCACGCCACCCTCGCCGGCCACACCTTC
				GAGCATGAGCTCACCGTGGCGGTCGATAACGGCTTCCTCGGCTCCATCGACGCCAACCGT
				GGCGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGGTGGATCCGTACGACCTCACC
				CAGGCCATGATGCAGATCATCCGCAACGGCGGCTTCAAGGACGGCGGCACCAACTTCGAC
				GCCAAACTCCGCCGCTCCTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCACGCGCTCCTGAACGCCGCCGCCGTCATCGAGGAGAGCCCGCTC
				TGCAAGATGGTCGAGGAGCGCTACGCTTCCTTCGACAGCGGTCTCGGCAAGCAGTTCGAG
				GAAGGCAAGGCCACCCTTGAGGACCTCTACGAGTATGCCAAGAAGAACGGCGAGCCCGTC
				GTCGCTTCCGGCAAGCAGGAGCTCTACGAGACCCTTCTGAACCTCTACGCGAAGTAG
5586MI206_004	Prevotella	Amino	116	MAKEYFPTIGKIPFEGVESKNPMAFHYYDAKRVVMGKPMKDWLKFAMAWWHTLGQASGDP
		Acid		FGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYYCFHDIDLVEDTEDIAEYEARM
				KDITDYLVEKQKETGIKNLWGTANVFGNKRYMNGAATNPQFDVVARAAVQIKNAIDATIK
				LGGTSYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAHGEKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANR
				GDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVIEESPLCKMVEERYASFDSGLGKQFEEGKATLEDLYEYAKKNGEPV
				VASGKQELYETLLNLYAK
5586MI208_003	Prevotella	DNA	117	ATGTCAACTGAGTATTTCCCTACAATCGGCAAGATTCCCTTCGAGGGACCCGAGAGCAAG
				AACCCCATGGCCTTCCACTACTATGAACCCGAAAAGTTGGTGATGGGCAAGAAGATGAAG
				GACTGGCTGCGTTTCGCAATGGCCTGGTGGCACACCCTTGGAGCCGCATCCGGCGACCAG
				TTCGGCGGACAGACCCGCAGTTACGCCTGGGACAAGGGCGACTGCCCTTACAGCCGCGCC
				CGCGCCAAGGTCGACGCCGGCTTCGAGATCATGCAGAAGCTCGGCATAGAGTTCTTCTGC
				TTCCATGACATCGACCTGGTCGAGGATACCGACGACATCGCCGAGTATGAAGCCCGGATG
				AAAGACATCACGGACTATCTGCTGGAAAAGATGGAGGCTACCGGCATCAAGAACCTCTGG
				GGCACGGCCAATGTCTTCGGTCACAAGCGTTATATGAACGGTGCAGCCACAAACCCCGAT
				TTCGCAGTGGTCGCAAGGGCGGCCGTGCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTGGGTGGTGAGAACTATGTGTTCTGGGGTGGACGCGAGGGTTATATGAGCCTGCTCAAC
				ACCCAGATGCAGAGGGAGAAGGAACACCTTGCCAAGATGCTCACCGCCGCACGTGACTAT
				GCACGCGCCAAAGGTTTCAAGGGCACGTTCCTCATCGAACCCAAGCCGATGGAACCCACC
				AAGCACCAGTATGACCAGGATACCGAGACCGTTATCGGATTCCTCCGCAGCCACGGCCTG
				GACAAGGACTTCAAGGTCAACATCGAGGTGAACCACGCCACCCTGGCGGGCCATACCTTC
				GAGCACGAACTGGCCACCGCCGTCGACAACGGCATGCTCGGCAGCATCGACGCCAACCGC
				GGAGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGACAACTTCGAGCTCACG
				CTTGCCATGATGCAGATAATCCGCAACGGCGGCCTGGCACCGGGCGGTTCGAACTTCGAC
				GCAAAGCTGCGCCGCAATTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCGCGCCCTCGTCAACGCCGCCGCCATCCTCGGCGAGTCGCCCGTT
				CCGGCTATGGTCAAGGACCGCTATGCTTCGTTCGACTGCGGCAAGGGCAAGGACTTCGAA
				GACGGCAAACTGACTCTCGAAGACATCGTCGCCTACGCCAGGGAGAATGGCGAGCCGAAA
				CAGATTTCCGGCAAGCAGGAACTCTACGAAACTATCGTCGCTCTTTACTGCAAGTAA
5586MI208_003	Prevotella	Amino	118	MSTEYFPTIGKIPFEGPESKNPMAFHYYEPEKLVMGKKMKDWLRFAMAWWHTLGAASGDQ
		Acid		FGGQTRSYAWDKGDCPYSRARAKVDAGFEIMQKLGIEFFCFHDIDLVEDTDDIAEYEARM
				KDITDYLLEKMEATGIKNLWGTANVFGHKRYMNGAATNPDFAVVARAAVQIKNAIDATIK
				LGGENYVFWGGREGYMSLLNTQMQREKEHLAKMLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDQDTETVIGFLRSHGLDKDFKVNIEVNHATLAGHTFEHELATAVDNGMLGSIDANR
				GDAQNGWDTDQFPIDNFELTLAMMQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALVNAAAILGESPVPAMVKDRYASFDCGKGKDFEDGKLTLEDIVAYARENGEPK
				QISGKQELYETIVALYCK
5586MI210_002	Prevotella	DNA	119	ATGTCATATTTTCCTACTATCGGTAACATCCCCTTTGAGGGTGTAGAGAGCAAGAATCCC
				CTTGCCTTCCATTATTATGACGCTTCCCGCGTAGTTATGGGCAAGCCCATGAAGGAGTGG
				CTCAAGTTTGCCATGGCCTGGTGGCACACGCTGGGTCAGGCATCGGCCGACCCTTTCGGC
				GGACAAACCCGCAGCTATGCCTGGGACAAAGGCGAGTGCCCCTACTGCCGTGCCCGTGCC
				AAGGCCGACGCCGGCTTCGAGCTCATGCAGAAACTGGGCATCGAGTATTTCTGCTCCCAC
				GACATTGACCTCATCGAGGACTGCGACGACATTGCAGAGTACGAGGCCCGTCTGAAGGAC
				ATTACGGACTACCTCCTGGAGAAGATGAAGAAGACCGGTATCAAGAACCTGTGGGGTACG
				GCCAATGTGTTCGGTAACAAGCGTTACATGAACGGTGCTGCTACCAACCCTCAGTTTGAC
				GTTGTGGCCCGCGCTGCCGTCCAGATCAAGAACGCCATTGACGCTACCATCAAGCTGGGC
				GGTTCCAACTATGTGTTCTGGGGTGGCCGTGAGGGTTACTACACGCTTCTGAACACCCAG
				ATGCAGCGTGAGAAGAATCACCTGGCTGCCATGCTCAAGGCTGCCCGCGACTATGCCCGC
				GCCAACGGTTTCAAGGGCACCTTCCTCATTGAGCCCAAGCCCATGGAGCCCACCAAGCAC
				CAGTACGACGTAGACACGGAGACCGTGATTGGATTCCTCCGCGCCAACGGTCTGGAGAAG
				GACTTCAAGGTGAACATTGAGGTGAACCACGCTACTCTTGCCGGTCACACCTTCGAGCAC
				GAGCTCACCGTGGCCCGTGAGAACGGCTTCCTGGGTTCCATTGACGCCAACCGCGGAGAT
				GCCCAGAACGGCTGGGACACCGACCAGTTCCCGGTAGATGCCTTTGACCTCACCCAGGCC
				ATGATGCAGATTCTCCTCAACGGAGGCTCCGGCAATGGCGGTACCAACTTTGACGCCAAG
				CTGCGCCGTTCCTCCACCGACCCCGAGGACATCTTCATCGCGCACATCAGCGCCATGGAT
				GCCATGGCTCACGCCCTGCTCAATGCAGCTGCCGTGCTGGAGGAGAGCCCGCTTTGCAAG
				ATGGTCAAGGAGCGTTACGCTTCCTTCGACAGCGGTCTTGGCAAGCAGTTCGAGGAAGGA
				AAGGCTACGCTGGAAGATCTGTATGCCTATGCCGTCAAGAACGGTGAGCCCGTGGTGGCT
				TCCGGCAAGCAGGAACTGTACGAAACCTTCCTGAACCTCTATGCAAAATGGTAA
5586MI210_002	Prevotella	Amino	120	MSYFPTIGNIPFEGVESKNPLAFHYYDASRVVMGKPMKEWLKFAMAWWHTLGQASADPFG
		Acid		GQTRSYAWDKGECPYCRARAKADAGFELMQKLGIEYFCSHDIDLIEDCDDIAEYEARLKD
				ITDYLLEKMKKTGIKNLWGTANVFGNKRYMNGAATNPQFDVVARAAVQIKNAIDATIKLG
				GSNYVFWGGREGYYTLLNTQMQREKNHLAAMLKAARDYARANGFKGTFLIEPKPMEPTKH
				QYDVDTETVIGFLRANGLEKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGD
				AQNGWDTDQFPVDAFDLTQAMMQILLNGGSGNGGTNFDAKLRRSSTDPEDIFIAHISAMD
				AMAHALLNAAAVLEESPLCKMVKERYASFDSGLGKQFEEGKATLEDLYAYAVKNGEPVVA
				SGKQELYETFLNLYAKW
5586MI212_002	Prevotella	DNA	121	ATGTCAACTGAGTATTTCCCTACAATCGGCAAGATTCCCTTCGAGGGACCCGAGAGCAAG
				AACCCCATGGCCTTCCACTACTATGAACCCGAAAAGTTGGTGATGGGCAAGAAGATGAAG
				GACTGGCTGCGTTTCGCAATGGCCTGGTGGCACACCCTTGGAGCCGCATCCGGCGACCAG
				TTCGGCGGACAGACCCGCAGTTACGCCTGGGACAAGGGCGACTGCCCTTACAGCCGCGCC
				CGCGCCAAGGTCGACGCCGGCTTCGAGATCATGCAGAAGCTCGGCATAGAGTTCTTCTGC
				TTCCATGACATCGACCTGGTCGAGGATACCGACGACATCGCCGAGTATGAAGCCCGGATG
				AAAGACATCACGGACTATCTGCTGGAAAAGATGGAGGTTACCGGCATCAAGAACCTCTGG
				GGCACGGCCAATGTCTTCGGTCACAAGCGTTATATGAACGATGCAGCCACAAACCCCGAT
				TTCGCAGTGGTCGCAAGGGCGGCCGTGCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTGGGTGGTGAGAACTATGTGTTCTGGGGTGGACGCGAGGGTTATATGAGCCTGCTCAAC
				ACCCAGATGCAGAGGGAGAAGGAACACCTTGCCAAGATGCTCACCGCCGCACGTGACTAT
				GCACGCGCCAAAGGTTTCAAGGGCACGTTCCTCATCGAACCCGAGCCGATGGAACCCACC
				AAGCACCAGTATGACCAGGATACCGAGACCGTTATCGGATTCCTCCGCAGCCACGGCCTG
				GACAAGGACTTCAAGGTCAACATCGAGGTGAACCACGCCACCCTGGCGGGCCATACCTTC
				GAGCACGAACTGGCCACCGCCGTCGACAACGGCATGCTCGGCAGCATCGACGCCAACCGC
				GGAGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGACAACTTCGAGCTCACG
				CTTGCCATGATGCAGATAATCCGCAACGGCGGCCTGGCACCGGGCGGTTCGAACTTCGAC
				GCAAAGCTGCGCCGCAATTCCACCGATCCCGAGGACATCATCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCGCGCCCTCGTCAACGCCGCCGCCATCCTCGGCGAGTCGCCCGTT
				CCGGCTATGGTCAAGGACCGCTATGCTTCGTTCGACTGCGGCAAGGGCAAGGACTTCGAA
				GACGGCAAACTGACTCTCGAAGACATCGTCGCCTACGCCAGGGAGAATGGCGAGCCGAAA
				CAGATTTCCGGCAAGCAGGAACTCTACGAAACTATCGTCGCTCTTTACTGCAAGTAA
5586MI212_002	Prevotella	Amino	122	MSTEYFPTIGKIPFEGPESKNPMAFHYYEPEKLVMGKKMKDWLRFAMAWWHTLGAASGDQ
		Acid		FGGQTRSYAWDKGDCPYSRARAKVDAGFEIMQKLGIEFFCFHDIDLVEDTDDIAEYEARM
				KDITDYLLEKMEVTGIKNLWGTANVFGHKRYMNDAATNPDFAVVARAAVQIKNAIDATIK
				LGGENYVFWGGREGYMSLLNTQMQREKEHLAKMLTAARDYARAKGFKGTFLIEPEPMEPT
				KHQYDQDTETVIGFLRSHGLDKDFKVNIEVNHATLAGHTFEHELATAVDNGMLGSIDANR
				GDAQNGWDTDQFPIDNFELTLAMMQIIRNGGLAPGGSNFDAKLRRNSTDPEDIIIAHISA
				MDAMARALVNAAAILGESPVPAMVKDRYASFDCGKGKDFEDGKLTLEDIVAYARENGEPK
				QISGKQELYETIVALYCK
5586MI213_003	Prevotella	DNA	123	ATGACCAACGAGTATTTTCCCGGAATCGGTGTGATTCCGTTTGAAGGACAGGAAAGCAAG
				AATCCCCTGGCTTTCCATTATTATGACGCCAACCGCGTAGTGATGGGCAAACCCATGAAG
				GAATGGTTCAAATTTGCCATGGCCTGGTGGCATACGCTGGGGCAGGCATCGGCCGATCCC
				TTCGGCGGACAGACCCGCTCCTACGCATGGGACAAGGGCGAGTGCCCTTACTGCCGTGCC
				CGCCAGAAGGCCGACGCCGGCTTTGAACTGATGCAGAAGCTGGGAATCGGCTATTTCTGC
				TTCCACGATGTGGATATCATCGAGGACTGCGAGGACATTGCCGAGTATGAGGCCCGTATG
				AAGGACATCACGGACTATCTGCTGGTGAAGATGAAGGAAACGGGCATCAAGAATCTGTGG
				GGCACGGCCAACGTCTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAACCCGCAA
				TTCGACGTGGTAGCCCGCGCTGCGGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAG
				CTGGGCGGCAGCAATTATGTGTTCTGGGGCGGCCGGGAAGGCTACTACACCCTTTTGAAC
				ACGCAGATGCAGCGGGAGAAGGACCACCTGGCCCAGATGCTCAAGGCGGCCCGCGACTAT
				GCCCGCGGCAAGGGATTCAAGGGCACGTTCCTCATTGAGCCCAAGCCCATGGAGCCCACC
				AAGCACCAGTACGACGTAGATACGGAGACCGTGATTGGTTTCCTGCGCGCCAACGGGCTG
				GACAAGGACTTCAAGGTGAATATCGAAGTGAACCACGCCACCCTGGCCGGCCATACCTTC
				GAGCACGAGCTCACCGTGGCCCGCGAAAACGGCTTCCTGGGCAGCATCGACGCCAACCGC
				GGAGACGCCCAGAACGGCTGGGATACAGACCAGTTCCCCGTGGACGCCTTTGACCTCACC
				CAGGCCATGATGCAGGTCCTGCTCAACGGCGGATTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAACTGCGCCGTTCCTCCACGGATCCCGAGGACATCTTCATCGCCCACATCAGCGCC
				ATGGACGCCATGGCCCACGCCCTCCTGAACGCCGCCGCCATCCTGGAAGAGAGCCCCATG
				CCGGGCATGGTGAAGGAGCGCTACGCTTCCTTCGACAATGGCCTTGGCAAGAAGTTCGAG
				GAAGGAAAGGCCACGCTGGAAGAGCTGTACGACTATGCCAAGAAGAACGGCGAGCCTGTG
				GCCGCTTCCGGAAAGCAGGAACTGTACGAAACGCTGCTGAACCTGTACGCCAAGTAA
5586MI213_003	Prevotella	Amino	124	MTNEYFPGIGVIPFEGQESKNPLAFHYYDANRVVMGKPMKEWFKFAMAWWHTLGQASADP
		Acid		FGGQTRSYAWDKGECPYCRARQKADAGFELMQKLGIGYFCFHDVDIIEDCEDIAEYEARM
				KDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPQEDVVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLAQMLKAARDYARGKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGELRANGLDKDEKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDAFDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPMPGMVKERYASFDNGLGKKFEEGKATLEELYDYAKKNGEPV
				AASGKQELYETLLNLYAK
5586MI215_003	Prevotella	DNA	125	ATGGCAAAAGAGTATTTCCCGCAGATCGGAAAGATCGGCTTTGAGGGTCTTGAGAGCAAG
				AACCCGATGGCATTCCATTATTATGACGCCGAGCGTGTCGTGCTCGGAAAGAAGATGAAG
				GACTGGCTGAAGTTCGCGATGGCCTGGTGGCATACGCTCGGACAGGCTTCCGGCGACCCA
				TTCGGCGGCCAGACTCGCAGCTATGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGTGCC
				CGCGCCAAGGCCGACGCCGGCTTCGAGCTCATGCAGAAGCTCGGCATCGAGTACTTCTGC
				TTCCACGACATCGACCTCATCGAGGACTGCGACGACATCGACGAGTACGAGGCCCGGATG
				AAGGACATCACCGACTACCTGCTGGAGAAGATGAAGGAGACCGGAATCAAGAATCTCTGG
				GGAACGGCCAACGTCTTCGGTCACAAGCGCTACATGAACGGCGCCGCTACCAATCCGCAG
				TTTGAAATCGTCGCCCGCGCTGCCGTCCAGATCAAGAACGCGCTCGACGCCACCATCAAG
				CTCGGCGGCTCCAACTACGTCTTCTGGGGCGGCCGCGAGGGCTATTACACGCTGCTGAAT
				ACCCAGATGCAGCGCGAGAAGGACCATCTCGCCAGGCTCCTTACCGCCGCCCGCGACTAT
				GCGCGCGCCAAGGGGTTCAAGGGGACCTTCCCCATCGAGCCGAAGCCGATGGAGCCGACC
				AAGCACCAGTATGACGTCGACACGGAGACCGTCATCGGTTTCCTCCGCCAGAATGGCCTC
				GACAAGGACTTCAAGGTCAATATCGAGGTGAACCACGCCACCCTCGCCGGCCATACCTTC
				GAGCACGAGCTGACCGCGGCCCGGGAGAACGGCTTCCTCGGCAGCATCGACGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGGTGGACGCCTTCGATCTCACG
				CGGGCCATGATGCAGATCCTGCTCAATGGCGGTTTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAGCTGCGCCGCAGCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCACGCCCTGCTGAATGCGGCCGCCATCCTCGAGGAAAGCCCGCTG
				CCGGCCCTGGTCAAGCAGCGCTATGCGTCCTTCGACAGCGGTCTCGGCAAGCAGTTCGAG
				GAGGGTAAGGCCACGCTCGAGGACCTGTACGCATACGCGAAGGAGCACGGCGAGCCCGTC
				GCGGCCTCCGGCAAGCAGGAGCTCTGCGAGACCTATCTCAACCTCTACGCGAAATAA
5586MI215_003	Prevotella	Amino	126	MAKEYFPQIGKIGFEGLESKNPMAFHYYDAERVVLGKKMKDWLKFAMANWHTLGQASGDP
		Acid		FGGQTRSYENDKGECPYCRARAKADAGFELMQKLGIEYFCFHDIDLIEDCDDIDEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAATNPQFEIVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLARLLTAARDYARAKGFKGTFPIEPKPMEPT
				KHQYDVDTETVIGFLRQNGLDKDEKVNIEVNHATLAGHTFEHELTAARENGFLGSIDANR
				GDAQNGWDTDQFPVDAFDLTRAMMQILLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPLPALVKQRYASFDSGLGKQFEEGKATLEDLYAYAKEHGEPV
				AASGKQELCETYLNLYAK
5607MI1_003	Prevotella	DNA	127	ATGAGTAAAGAGTATTTTCCTGGGATTGGCAAAATCCCGTATGAGGGAGCCGAGAGCAAG
				AATGTGATGGCATTCCACTATTATGATCCCGAACGCGTGGTCATGGGCAAGAAAATGAAA
				GACTGGTTCAAGTTCGCTATTGCCTGGTGGCATACCCTGGGGCAGGCCAGTGCTGACCAG
				TTTGGCGGACAGACCCGTTTCTATGAATGGGACAAAGCCGAGGACCCCTTGCAGCGTGCC
				AAGGACAAGATGGATGCCGGTTTTGAAATCATGCAGAAGCTGGGCATCGAGTATTTCTGT
				TTCCATGATGTGGACCTCATCGAGGAGGCCGATACCATCGAGGAATATGAAGCCCGCATG
				CAGGCGATTACCGACTACGCGCTGGAGAAGATGAAGGCAACGGGTATCAAGTTGCTGTGG
				GGCACTGCCAACGTGTTCGGCCACAAGCGTTACATGAACGGCGCCGCCACCAATCCCGAC
				TTCAATGTCGTGGCACGTGCAGCCGTGCAGATCAAGAACGCCCTCGATGCTACCATCAAG
				TTGGGCGGAACGAGCTACGTCTTCTGGGGCGGTCGTGAAGGCTATCAGAGCCTGCTCAAC
				ACCCAGATGCAGCGCGAGAAGAACCACCTGGCCAAGATGCTCACGGCAGCCCGTGACTAT
				GCCCGTGCTAAGGGCTTCAAGGGCACCTTCCTGATTGAGCCCAAGCCGATGGAACCCACC
				AAGCACCAGTATGACCAGGACACCGAGACCGTTATCGGCTTCTTGCGTGCCAATGGCCTT
				GACAAGGACTTTAAGGTCAACATTGAGGTCAACCATGCCACGCTGGCTGGCCACACCTTT
				GCACATGAGTTGGCAGTGGCTGTGGATAACGGTATGCTGGGCAGCATCGATGCTAACCGT
				GGTGACCACCAGAACGGCTGGGATACAGACCAGTTCCCCATCAACAGTTATGAACTCACC
				AATGCTATGCTGCAGATCATGCACGGCGGCGGTTTCAAGGACGGCGGTACCAACTTTGAC
				GCCAAGCTGCGCCGCAACAGTACCGACCCCGAGGACATCTTTACCGCTCACATCAGTGGT
				ATGGACGCTCTGGCCCGTGCCCTGTTGAGTGCTGCCGATATCCTTGAGAAGAGCGAGTTG
				CCTGAAATGCTCAAGGAACGCTATGCCAGCTTTGACGCGGGTGAAGGCAAGCGCTTTGAG
				GATGGCCAGATGACTCTTGAGGAACTGGTTGCCTATGCCAAGTCCCATGGCGAGCCTGCT
				ACCATCAGTGGCAAGCAGGAAAAATATGAAGCCATCGTGGCTTTGCACGTCAAGTAA
5607MI1_003	Prevotella	Amino	128	MSKEYFPGIGKIPYEGAESKNVMAFHYYDPERVVMGKKMKDWFKFAIAWWHTLGQASADQ
		Acid		FGGQTREYEWDKAEDPLQRAKDKMDAGFEIMQKLGIEYFCFHDVDLIEEADTIEEYEARM
				QAITDYALEKMKATGIKLLWGTANVFGHKRYMNGAATNPDFNVVARAAVQIKNALDATIK
				LGGTSYVFWGGREGYQSLLNTQMQREKNHLAKMLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDQDTETVIGFLRANGLDKDEKVNIEVNHATLAGHTFAHELAVAVDNGMLGSIDANR
				GDHQNGWDTDQFPINSYELTNAMLQIMHGGGFKDGGTNFDAKLRRNSTDPEDIFTAHISG
				MDALARALLSAADILEKSELPEMLKERYASFDAGEGKRFEDGQMTLEELVAYAKSHGEPA
				TISGKQEKYEAIVALHVK
5607MI2_003	Prevotella	DNA	129	ATGAGTAAAGAGTATTATCCTGAGATTGGCAAAATCCCGTTTGAGGGTCCCGAGAGCAAG
				AATGTGATGGCGTTCCATTACTATGAACCCGAACGCGTCGTCATGGGTAAGAAGATGAAA
				GACTGGCTCAAGTTTGCCATGTGCTGGTGGCACAGCCTGGGTCAGGCCAGTGCCGACCAG
				TTCGGCGGACAGACACGTTTCTACGAGTGGGACAAGGCCGATACCCCCCTGCAGCGTGCC
				AAGGACAAAATGGATGCCGGATTTGAAATCATGCAGAAGTTGGGCATCGAGTACTTCTGC
				TTCCACGATGTGGACCTCATCGAGGAGGCCGATACCATCGAGGAATACGAGGCCCGCATG
				AAGGCCATTACCGACTATGCGCTGGAGAAGATGCAGGCCACCGGCATCAAGTTGCTGTGG
				GGCACTGCCAATGTGTTCGGCCACAAGCGCTACATGAACGGCGCCGCCACCAATCCCGAT
				TTCAATGTCGTGGCACGTGCCGCCGTCCAAATCAAGAATGCCATCGATGCCACCATCAAG
				CTGGGCGGCACGAGTTACGTCTTCTGGGGTGGTCGTGAGGGCTATCAGAGTCTGCTCAAC
				ACGCAGATGCAGCGCGAGAAGGACCATCTGGCCCGCATGCTGGCGGCAGCCCGCGACTAT
				GGCCGTGCCCATGGCTTCAAGGGCACTTTCCTGATCGAGCCCAAACCCATGGAGCCCACC
				AAGCACCAGTATGATGTGGACACCGAGACCGTGCTCGGCTTCCTGCGTGCCCACGGCCTG
				GACAAGGACTTCAAGGTTAACATCGAGGTCAATCATGCTACGCTGGCGGGACACACTTTC
				AGCCACGAACTGGCTGTGGCCGTGGACAACGGTATGCTGGGCAGCATCGACGCCAACCGC
				GGCGATTATCAGAATGGCTGGGACACCGACCAGTTCCCCATCGACAGCTTCGAGCTCACC
				CAGGCCATGCTGCAGATCATGCGCGGCGGCGGCTTCAAGGACGGAGGTACCAACTTCGAT
				GCCAAGCTGCGTCGCAACAGTACCGACCCTGAGGACATCTTCATCGCCCACATCAGCGGT
				ATGGATGCCATGGCACGCGGCCTGTTGAGCGCTGCCGCTATCCTCGAGGATGGCGAGTTG
				CCCGCGATGCTCAAGGCACGTTATGCCAGCTTTGACCAGGGCGAGGGTAAGCGCTTTGAG
				GACGGCGAGATGACGCTCGAGCAGCTGGTGGATTATGCAAAGGATTATGCCAAATCGCAC
				GGCGAGCCTGATGTCATCAGCGGCAAGCAGGAGAAGTTTGAAACCATCGTGGCCCTTTAC
				GCCAAGTAA
5607MI2_003	Prevotella	Amino	130	MSKEYYPEIGKIPFEGPESKNVMAFHYYEPERVVMGKKMKDWLKFAMCWWHSLGQASADQ
		Acid		FGGQTRFYEWDKADTPLQRAKDKMDAGFEIMQKLGIEYFCFHDVDLIEEADTIEEYEARM
				KAITDYALEKMQATGIKLLWGTANVFGHKRYMNGAATNPDFNVVARAAVQIKNAIDATIK
				LGGTSYVFWGGREGYQSLLNTQMQREKDHLARMLAAARDYGRAHGFKGTFLIEPKPMEPT
				KHQYDVDTETVLGFLRAHGLDKDFKVNIEVNHATLAGHTFSHELAVAVDNGMLGSIDANR
				GDYQNGWDTDQFPIDSFELTQAMLQIMRGGGFKDGGTNFDAKLRRNSTDPEDIFIAHISG
				MDAMARGLLSAAAILEDGELPAMLKARYASFDQGEGKRFEDGEMTLEQLVDYAKDYAKSH
				GEPDVISGKQEKFETIVALYAK
5607M13_003	Prevotella	DNA	131	ATGACCAACGAGTATTTTCCCGGAATCGGTGTGATTCCGTTTGAAGGACAGGAAAGCAAG
				AATCCCCTGGCTTTCCATTATTATGACGCCAACCGCGTAGTGATGGGCAAACCCATGAAG
				GAATGGTTCAAATTTGCCATGGCCTGGTGGCATACGCTGGGGCAGGCATCGGCCGATCCC
				TTCGGCGGACAGACCCGCTCCTACGCATGGGACAAGGGCGAGTGCCCTTACTGCCGTGCC
				CGCCAGAAGGCCGACGCCGGCTTTGAACTGATGCAGAAGCTGGGAATCGGCTATTTCTGC
				TTCCACGATGTGGATATCATCGAGGACTGCGAGGACATTGCCGAGTATGAGGCCCGTATG
				AAGGACATCACGGACTATCTGCTGGTGAAGATGAAGGAAACGGGCATCAAGAATCTGTGG
				GGCACGGCCAACGTCTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAACCCGCAA
				TTCGACGTGGTAGCCCGCGCTGCGGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAG
				CTGGGCGGCAGCAATTATGTGTTCTGGGGCGGCCGGGAAGGCTACTACACCCTTTTGAAC
				ACGCAGATGCAGCGGGAGAAGGACCACCTGGCCCAGATGCTCAAGGCGGCCCGCGACTAT
				GCCCGCGGCAAGGGATTCAAGGGCACGTTCCTCATTGAGCCCAAGCCCATGGAGCCCACC
				AAGCACCAGTACGACGTAGATACGGAGACCGTGATTGGTTTCCTGCGCGCCAACGGGCCG
				GACAAGGACTTCAAGGTGAATATCGAAGTGAACCACGCCACCCTGGCCGGCCATACCTTC
				GAGCACGAGCTCACCGTGGCCCGCGAAAACGGCTTCCTGGGCAGCATCGACGCCAACCGC
				GGAGACGCCCAGAACGGCTGGGATACAGACCAGTTCCCCGTGGACGCCTTTGACCTCACC
				CAGGCCATGATGCAGGTCCTGCTCAACGGCGGATTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAACTGCGCCGTTCCTCCACGGATCCCGAGGACATCTTCATCGCCCACATCAGCGCC
				ATGGACGCCATGGCCCACGCCCTCCTGAACGCCGCCGCCATCCTGGAAGAGAGCCCCATG
				CCGGGCATGGTGAAGGAGCGCTACGCTTCCTTCGACAATGGCCTTGGCAAGAAGTTCGAG
				GAAGGAAAGGCCACGCTGGAAGAGCTGTACGACTATGCCAAGAAGAACGGCGAGCCTGTG
				GCCGCTTCCGGAAAGCAGGAACTGTACGAAACGCTGCTGAACCTGTACGCCAAGTAA
5607M13_003	Prevotella	Amino	132	MTNEYFPGIGVIPFEGQESKNPLAFHYYDANRVVMGKPMKEWFKFAMAWWHTLGQASADP
		Acid		FGGQTRSYAWDKGECPYCRARQKADAGFELMQKLGIGYFCFHDVDIIEDCEDIAEYEARM
				KDITDYLLVKMKETGIKNLWGTANVEGHKRYMNGAATNPQEDVVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLAQMLKAARDYARGKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGPDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDAFDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPMPGMVKERYASFDNGLGKKFEEGKATLEELYDYAKKNGEPV
				AASGKQELYETLLNLYAK
5607M14_005	Prevotella	DNA	133	ATGACTAAAGAGTATTTCCCTTCCGTCGGCAAGATTGCCTTTGAAGGACCCGAAAGCAAG
				AACCCTATGGCCTTCCATTATTATGACGCCAATCGCGTGGTAATGGGAAAGCCGATGAAA
				GAATGGCTTAAATTTGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCTGCAGACCCC
				TTCGGCGGTCAGACCCGCTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCC
				AAGGCCAAGGCCGATGCCGGCTTTGAACTGATGCAGAAACTGGGCATCGAGTATTTCTGC
				TTCCACGATATAGACCTGGTGGAAGACTGCGATGATATCGCCGAATACGAGGCCCGCATG
				AAGGACATCACGGACTATCTCCTGGAGAAGATGAAGGAAACCGGCATCAAGAACCTCTGG
				GGAACCGCCAACGTGTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAACCCTCAG
				TTCGACATCGTGGCCCGTGCCGCTGTCCAGATCAAGAACGCCCTGGATGCCACCATCAAG
				CTGGGCGGCTCCAACTATGTGTTCTGGGGCGGCCGTGAGGGCTACTATACCCTCCTGAAC
				ACCCAGATGCAGAGAGAGAAGGACCACCTGGCCAAGATGCTCACCGCCGCCCGCGACTAT
				GCCCGTGCCAAGGGCTTCAAGGGCACCTTCCTCATCGAACCCAAGCCGATGGAGCCCACC
				AAGCACCAGTACGACGTAGATACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAATATTGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTC
				GAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCAGCATCGACGCCAACCGC
				GGAGACGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTGGATGCCTTCGACCTCACC
				CAGGCTATGATGCAGATCCTTCTGAACGGAGGCTTCGGCAACGGCGGTACCAACTTCGAC
				GCCAAACTGCGCCGCTCCTCCACGGACCCCGAGGACATCTTCATCGCCCACATCAGCGCT
				ATGGATGCCATGGCCCACGCCCTGCTGAATGCAGCCGCCATCCTGGAGGAAAGCCCGCTT
				CCGAAGATGCTGAAAGAGCGTTATGCCAGCTTTGACGGCGGTCTGGGCAAGAAGTTCGAA
				GAAGGCAAGGCCTCTCTGGAAGAACTCTACGAGTATGCCAAGAGCAACGGAGAGCCCGTG
				GCCGCTTCCGGCAAGCAGGAGCTCTGCGAAACGTACCTGAACCTCTACGCTAAGTAA
5607M14_005	Prevotella	Amino	134	MTKEYFPSVGKIAFEGPESKNPMAFHYYDANRVVMGKPMKEWLKFAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKGECPYCRAKAKADAGFELMQKLGIEYFCFHDIDLVEDCDDIAEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDIVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDAFDLTQAMMQILLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPLPKMLKERYASFDGGLGKKFEEGKASLEELYEYAKSNGEPV
				AASGKQELCETYLNLYAK
5607M15_002	Prevotella	DNA	135	ATGGCTAAAGAATACTTCCCCTCCATCGGCAAAATCCCTTTTGAAGGAGCCGACAGCAAA
				AATCCCCTCGCTTTCCATTATTATGACGCCGGACGCGTGGTTATGGGCAAGCCCATGAAG
				GAATGGCTTAAATTCGCCATGGCCTGGTGGCACACGCTGGGCCAGGCCTCCGGAGACCCC
				TTCGGCGGCCAGACCCGCAGCTACGAATGGGACAAGGGCGAATGCCCCTACTGCCGCGCC
				AAGGCCAAGGCCGACGCCGGTTTTGAAATCATGCAAAAGCTGGGCATCGAATACTTCTGC
				TTCCACGATGTGGACCTTATCGAGGATTGCGATGACATTGCCGAATACGAAGCCCGCATG
				AAGGACATCACGGACTACCTGCTGGAAAAGATGAAGGAGACCGGCATCAAGAACCTCTGG
				GGCACCGCCAATGTCTTCGGCCACAAGCGCTACATGAACGGCGCCGGCACCAATCCGCAG
				TTCGATGTGGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAG
				CTGGGCGGCTCCAACTATGTGTTCTGGGGCGGCCGCGAAGGCTATTACACCCTCCTCAAC
				ACACAGATGCAGCGGGAAAAAGACCACCTGGCCAAGTTGCTGACGGCCGCCCGCGACTAT
				GCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAACCCACC
				AAGCACCAGTACGACGTGGATACGGAGACGGTCATCGGCTTCCTCCGTGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTC
				GAGCATGAGCTCACCGTGGCCCGCGAGAACGGTTTCCTGGGCTCCATCGATGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGACACGGACCAGTTCCCTGTGGACCCGTACGATCTTACC
				CAGGCCATGATGCAGGTGCTGCTGAACGGCGGCTTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAACTCCGCCGCTCCTCCACCGACCCTGAGGACATCTTCATCGCCCATATTTCCGCC
				ATGGATGCCATGGCCCACGCTTTGCTTAACGCAGCTGCCGTGCTGGAAGAGAGCCCCCTG
				TGCCAGATGGTCAAGGAGCGTTATGCCAGCTTCGACGATGGCCTCGGCAAACAGTTCGAG
				GAAGGCAAGGCTACCCTGGAAGACCTGTACGAATACGCCAAGGCCCAGGGTGAACCCGTT
				GTCGCCTCCGGCAAGCAGGAGCTTTACGAGACTCTCCTGAACCTGTATGCCGTCAAGTAA
5607M15_002	Prevotella	Amino	136	MAKEYFPSIGKIPFEGADSKNPLAFHYYDAGRVVMGKPMKEWLKFAMAWWHTLGQASGDP
		Acid		FGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYFCFHDVDLIEDCDDIAEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAGTNPQFDVVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLAKLLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDPYDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVLEESPLCQMVKERYASFDDGLGKQFEEGKATLEDLYEYAKAQGEPV
				VASGKQELYETLLNLYAVK
5607M16_002	Prevotella	DNA	137	ATGACCAAAGAATATTTCCCTACCGTCGGGAAGATCCCCTTCGAGGGCCCCGAAAGCAAG
				AACCCTATGGCGTTCCATTACTATGACCCCAACCGTCTGGTGATGGGCAAGAAGATGAAA
				GACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTCGGCCAGGCGTCGGGCGACCAG
				TTCGGCGGCCAGACCCGCAGTTATGCGTGGGACGAGGGAGAATGCCCGTACGAGCGCGCC
				CGTGCCAAGGCTGACGCCGGCTTCGAGATCATGCAGAAACTCGGTATCGAGTTCTTCTGC
				TTCCACGACATCGACCTGATCGAGGATACCGACGACATCGCCGAGTATGAGGCCCGCCTG
				AAAGACATCACGGACTATCTGCTCGAGAAGATGAAAGCCACTGGCATCAAAAATCTCTGG
				GGAACGGCCAACGTGTTCGGCCACAAGCGTTGCATGAACGGCGCCGCCACCAACCCGGAC
				TTCGCCGTGCTGGCCCGCGCTGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTGGGCGGCGAGAACTATGTGTTCTGGGGTGGCCGCGAAGGCTACACGAGCCTGCTCAAC
				ACCCAGATGCAGCGTGAGAAAGAGCACCTGGGCCGCCTGCTGTCCCTGGCCCGCGACTAT
				GGCCGCGCCCACGGCTTCAAGGGTACCTTCCTGATCGAGCCCAAGCCGATGGGACCGACG
				AAACACCAGTACGACCAGGATACGGAAACTGTCATCGGTTTCCTGCGCCGCCACGGTCTA
				GACAAGGACTTCAAGGTCAATATCGAGGTGAACCATGCCACGCTGGCGGGCCACACCTTC
				GAACACGAACTGGCCTGCGCCGTGGATCACGGTATGCTGGGCAGCATCGACGCCAACCGC
				GGTGACGCACAGAACGGCTGGGATACCGACCAGTTCCCGATCGACAACTTCGAGCTGACC
				CTTTCCATGCTCCAGATCATCCGCAACGGTGGCCTGGCACCCGGCGGCTCGAATTTCGAT
				GCCAAGCTGCGCCGCAACTCCACCGATCCCGAAGACATTTTCATCGCGCACATCAGCGCC
				ATGGACGCCATGGCCCGCGCATTGGTCAATGCGGCCGCCATCCTGGAGGAGAGCGCTATT
				CCGAAGATGGTCAAGGAGCGTTACGCTTCGTTCGACAGCGGCAAAGGCAAGGAATACGAG
				GAAGGCAAGCTGACGCTCGAAGACATCGTGGCCTATGCCAAGGCGAACGGAGAACCGAAG
				CAGATTTCCGGCAAACAGGAACTCTACGAGACGCTTGTCGCACTCTATAGCAAATAA
5607M16_002	Prevotella	Amino	138	MTKEYFPTVGKIPFEGPESKNPMAFHYYDPNRLVMGKKMKDWLRFAMAWWHTLGQASGDQ
		Acid		FGGQTRSYAWDEGECPYERARAKADAGFEIMQKLGIEFFCFHDIDLIEDTDDIAEYEARL
				KDITDYLLEKMKATGIKNLWGTANVFGHKRCMNGAATNPDFAVLARAAVQIKNAIDATIK
				LGGENYVFWGGREGYTSLLNTQMQREKEHLGRLLSLARDYGRAHGFKGTFLIEPKPMGPT
				KHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANR
				GDAQNGWDTDQFPIDNFELTLSMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALVNAAAILEESAIPKMVKERYASFDSGKGKEYEEGKLTLEDIVAYAKANGEPK
				QISGKQELYETLVALYSK
5607M17_002	Prevotella	DNA	139	ATGACCAAAGGGTATTTCCCTACCATCGGCAGGATTCCCTTCGAGGGAACTGAAAGCAAG
				AATCCCCTCGCATTCCATTACTATGAGCCCGACCGGCTCGTACTGGGCAAGAAAATGAAA
				GACTGGCTGCGTTTCGCGATGGCCTGGTGGCACACCCTGGGCCAGGCGTCCGGCGACCAG
				TTCGGCGGCCAGACCCGCAGCTATGCCTGGGACAAGGCCGAGTGCCCCTATGAGCGCGCC
				AAGGCCAAAGCCGACGCCGGCTTCGAGATCATGCAGAAACTCGGCATCGAGTTCTTCTGT
				TTCCACGACATTGACCTCGTTGAGGATACCGACGACATCGCCGAGTATGAGGCCCGGATG
				AAGGACATTACCGACTATCTCCTGGTCAAGATGAAGGAGACCGGAATCAAGAACCTCTGG
				GGTACGGCCAATGTCTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAATCCCGAC
				TTCGACGTGGTGGCCCGCGCCGCCGTCCAGATCAAGAACGCCCTCGATGCCACCATCAAG
				CTGGGCGGTGAAAACTATGTGTTCTGGGGCGGCCGCGAAGGCTATATGAGCCTGCTCAAC
				ACGCAGATGCAGCGTGAGAAGGAGCACCTGGGCCGGATGCTGGTCGCCGCCCGCGACTAC
				GCCCGCGCCCACGGCTTCAAGGGTACCTTCCTCATCGAGCCCAAACCGATGGAACCGACC
				AAGCACCAGTACGACCAGGATACGGAAACCGTGATCGGCTTCCTTCGCCGCCACGGCCTG
				GACAAGGATTTCAAGGTGAACATCGAAGTGAACCACGCCACGCTGGCCGGCCACACCTTC
				GAGCACGAACTGGCCACCGCCGTCGACTGCGGCCTGCTGGGCAGCATCGACGCCAATCGC
				GGCGACGCTCAGAACGGCTGGGATACCGACCAGTTCCCGATCGACAACTTCGAACTCACG
				CTGGCCATGCTGCAGATTATCCGCAACGGCGGTCTGGCACCCGGCGGCTCGAACTTCGAC
				GCCAAACTGCGCCGTAACTCCACCGATCCGGAAGATATCTTCATCGCCCACATCAGTGCG
				ATGGACGCGATGGCCCGTGCGCTGGTCAACGCCGCCGCAATCTGGGAAGAGTCTCCCATC
				CCGCAGATGAAGAAAGAACGCTACGCGTCGTTCGACAGCGGCAAGGGCAAGGAATTCGAA
				GAGGGCAAGCTCTGCCTCGAAGACCTCGTGGCCTATGCCAAGGCGAACGGAGAACCGAAA
				CAGATCTCCGGCAGGCAGGAACTATATGAGACCATCGTCGCCCTTTATTGCAAATAG
5607M17_002	Prevotella	Amino	140	MTKGYFPTIGRIPFEGTESKNPLAFHYYEPDRLVLGKKMKDWLRFAMAWWHTLGQASGDQ
		Acid		FGGQTRSYAWDKAECPYERAKAKADAGFEIMQKLGIEFFCFHDIDLVEDTDDIAEYEARM
				KDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNALDATIK
				LGGENYVFWGGREGYMSLLNTQMQREKEHLGRMLVAARDYARAHGFKGTFLIEPKPMEPT
				KHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFERELATAVDCGLLGSIDANR
				GDAQNGWDTDQFPIDNFELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALVNAAAIWEESPIPQMKKERYASFDSGKGKEFEEGKLCLEDLVAYAKANGEPK
				QISGRQELYETIVALYCK
5608MI1_004	Prevotella	DNA	141	ATGACCAACGAGTATTTTCCCGGAATCGGTGTGATTCCGTTTGAAGGACAGGAAAGCAAG
				AATCCCATGGCTTTCCATTATTATGACGCCAACCGCGTAGTGATGGGCAAACCCATGAAG
				GAATGGTTCAAATTTGCCATGGCCTGGTGGCATACGCTGGGGCAGGCATCGGCCGATCCC
				TTCGGCGGACAGACCCGCTCCTACGCATGGGACAAGGGCGAGTGCCCTTACTGCCGTGCC
				CGCCAGAAGGCCGACGCCGGCTTTGAACTGATGCAGAAGCTGGGTATCGGCTATTTCTGC
				TTCCACGATGTGGATATCATCGAGGACTGCGAAGACATTGCCGAGTATGAGGCCCGTATG
				AAGGACATCACGGACTATCTGCTGGTGAAGATGAAGGAAACGGGCATCAAGAACCTGTGG
				GGCACGGCCAACGTCTTCGGCCACAAGCGCTATATGAACGGCGCTGCCACCAACCCGCAG
				TTCGACGTGGTGGCCCGCGCTGCGGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAG
				CTGGGCGGCAGCAATTACGTGTTCTGGGGCGGCCGCGAAGGCTATTATACCCTTTGGAAC
				ACGCAGATGCGGCGGGAGAAGGACCACCTGGCCCAGATGCTCAAGGCAGCCCGTGACTAT
				GCCCGCGGCAAGGGATTCAAGGGCACGTTCCTCATTGAGCCCAAGCCCATGGAGCCCACC
				AAGCACCAGTACGACGTAGATACGGAGACCGTGATTGGCTTCCTGCGCGCAAACGGACTG
				GACAAGGACTTCAAGGTGAATATCGAAGTGAACCACGCCACCCTGGCCGGCCACACCTTC
				GAGCACGAACTCACCGTGGCCCGCGAAAACGGCTTCCTGGGCAGCATCGACGCCAACCGC
				GGAGACGCCCAGAACGGTTGGGATACAGACCAGTTCCCCATAGATGCCTTTGACCTCACC
				CAGGCCATGATGCAGGTCCTGCTCAACGGCGGATTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAACTGCGCCGTTCCTCCACGGATCCCGAGGACATCTTCATCGCCCACATCGGCGCC
				ATGGACGCCATGGCCCACGCCCTCCTGAACGCCGCCGCCATCCTGGAAGAGAGCCCCATG
				CCGGGCATGGTGAAGGAGCGCTACGCTTCCTTCGACAATGGCCTTGGCAAGAAGTTCGAG
				GAAGGAAAGGCCACGCTGGAAGAGCTGTACGACTATGCCAAGAAGAACGGCGAGCCTGTG
				GCCGCTTCCGGCAAGCAGGAACTGTACGAAACGCTGCTGAACCTGTACGCCAAGTAA
5608MI1_004	Prevotella	Amino	142	MTNEYFPGIGVIPFEGQESKNPMAFHYYDANRVVMGKPMKEWFKFAMAWWHTLGQASADP
		Acid		FGGQTRSYAWDKGECPYCRARQKADAGFELMQKLGIGYFCFHDVDIIEDCEDIAEYEARM
				KDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDVVARAAVQIKNALDATIK
				LGGSNYVFWGGREGYYTLWNTQMRREKDHLAQMLKAARDYARGKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPIDAFDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHIGA
				MDAMAHALLNAAAILEESPMPGMVKERYASFDNGLGKKFEEGKATLEELYDYAKKNGEPV
				AASGKQELYETLLNLYAK
5608MI2_002	Prevotella	DNA	143	ATGAAAGAATACTTCCCTACCATCGGAAAAATCCCTTTCGAGGGCCCTCAGAGCAAGAAT
				CCGCTCGCATTCCATTACTATGACGCCAACCGCGTTGTCGCCGGCAAACCCATGAAGGAC
				TGGCTCAAGTTCGCCATGGCTTGGTGGCACACCCTGGGCGCAGCATCGGCAGACCCCTTC
				GGCGGCCAGACCCGCAGCTACGAGTGGGACAAAGCCGAGTGCCCTTACTGCCGTGCCCGT
				GAAAAGGCCGACGCCGGCTTCGAGATCATGCAGAAACTTGGAATCGAGTACTTCTGCTTC
				CATGACATCGACCTTGTGGAAGACTGCGAGGACATTGCCGAGTACGAGGCCCGCATGAAG
				GACATCACGGACTACCTCCTGGAGAAGATGAAGGCCACCGGCATCAAGAACCTGTGGGGC
				ACCGCCAACGTCTTTGGCAACAAGCGCTACATGAACGGCGCAGCCACCAACCCTCAGTTC
				GACATCGTTGCCCGTGCAGCTGTCCAGATCAAGAACGCCATCGACGCAACAATCAAGCTG
				GGCGGTACCGGTTACGTATTCTGGGGCGGCCGCGAGGGCTACTACACCCTCCTGAACACC
				CAGATGCAGCGCGAGAAGGACCACCTTGCCAAGATGCTCACCGCAGCCCGCGACTACGCC
				CGCGCCAAGGGATTCAAGGGCACATTCCTCATCGAGCCCAAGCCCATGGAGCCCACCAAG
				CACCAGTACGATGTTGACACGGAAACCGTCATCGGCTTCCTCCGCGCCAACGGCCTGGAC
				AAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTCGAG
				CACGAGCTCACCGTGGCCGTGGACAACGGCTTCCTGGGCAGCATCGACGCAAACCGCGGC
				GACGCCCAGAACGGCTGGGACACTGACCAGTTCCCTGTGGATCCTTACGACCTCACCCAG
				GCAATGATGCAGATTATCCGCAACGGCGGCTTCAAGGACGGCGGCACCAACTTCGACGCC
				AAACTCCGCCGCAGCTCCACGGACCCCGAGGACATCTTCATCGCCCACATCAGCGCAATG
				GATGCAATGGCACACGCCCTCATCAACGCTGCTGCAGTGCTTGAGGAAAGCCCTCTGTGC
				GAGATGGTTGCAAAGCGCTACGCCAGCTTTGACAGCGGTCTTGGCAAGAAGTTCGAGGAA
				GGCAAAGCCACTCTCGAGGAGATCTACGAGTATGCCAAGAAGGCCCCGGCACCCGTCGCC
				GCCTCCGGCAAGCAGGAGCTCTACGAGACACTGCTCAATCTGTACGCTAAATAA
5608MI2_002	Prevotella	Amino	144	MKEYFPTIGKIPFEGPQSKNPLAFHYYDANRVVAGKPMKDWLKFAMAWWHTLGAASADPF
		Acid		GGQTRSYEWDKAECPYCRAREKADAGFEIMQKLGIEYFCFHDIDLVEDCEDIAEYEARMK
				DITDYLLEKMKATGIKNLWGTANVFGNKRYMNGAATNPQFDIVARAAVQIKNAIDATIKL
				GGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTK
				HQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANRG
				DAQNGWDTDQFPVDPYDLTQAMMQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISAM
				DAMAHALINAAAVLEESPLCEMVAKRYASFDSGLGKKFEEGKATLEEIYEYAKKAPAPVA
				ASGKQELYETLLNLYAK
5608MI3_004	Prevotella	DNA	145	ATGACCAAAGAGTATTTCCCTACAATCGGAAAGATTCCCTTCGAAGGCCCGGAGAGCAAG
				AATCCGCTGGCATTCCATTACTATGAACCCGACAGAATCATCCTCGGCAGGAAGATGAAG
				GACTGGCTGCGCTTCGCCGTGGCCTGGTGGCACACCCTCGGCCAGGCGTCCGGCGACCAG
				TTCGGAGGCCAGACCCGCAACTATGCGTGGGACGAGCCCGAATGCCCGGTAGAGCGCGCG
				AAAGCCAAGGCCGACGCCGGCTTCGAGCTGATGCAGAAGCTGGGCATCGAGTATTTCTGC
				TTCCACGACGTAGACCTCATAGAGGAGGCCGCAACCATCGAAGAATATGAGGAGCGCATG
				GGCATCATAACCGACTACCTGCTCGGGAAGATGAAGGAGACAGGTATCAAGAACCTCTGG
				GGCACCGCCAACGTGTTCGGCCACAAGCGTTACATGAACGGAGCCGCCACCAACCCCGAC
				TTCGACGTGGTGGCCCGTGCGGCCGTGCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTGGGCGGCGAGAATTACGTATTCTGGGGCGGACGCGAGGGCTATGCAAGCCTGCTCAAC
				ACTCAGATGCAGCGCGAGAAAGACCACCTGGGACGCATGCTGGCTGCAGCCCGCGACTAT
				GGCCGCGCCCACGGATTCAAGGGCACTTTCCTCATCGAGCCCAAACCCATGGAGCCTACC
				AAGCACCAGTACGACCAGGATACCGAGACCGTTATCGCCTTCCTGCGCAGGAACGGCCTC
				GACAAGGATTTCAAGGTAAACATCGAGGTGAACCACGCCACCCTGGCGGGCCACACCTTC
				GAGCACGAACTGGCGGTGGCAGTGGACAACGGCCTGCTTGGCAGCATCGACGCCAACCGC
				GGCGACGCGCAGAACGGATGGGACACCGACCAGTTCCCCATCGACAACTTCGAGCTCACC
				CAGGCCATGCTGCAGATAATCCGCAACGGCGGACTGGGAACCGGCGGATCGAACTTCGAC
				GCCAAGCTGCGCCGCAATTCCACCGACCCTGAGGATATCTTCATCGCCCACATCAGTGCG
				ATGGACGCCATGGCACGCGCGCTGGCAAACGCCGCCGCAATCATCGAAGAGAGCCCCATC
				CCCGCAATGCTGAAGGAGCGCTACGCATCGTTCGACAGCGGCAAGGGCAAGGAGTTCGAG
				GACGGCAAACTGAGCCTCGAAGAACTGGTAGCCTACGCCAAGGCGAACGGCGAGCCGAAG
				CAGATTTCCGGCAAGCAGGAACTCTACGAAACCATAGTGGCCCTCTATTGCAAGTAA
5608MI3_004	Prevotella	Amino	146	MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRIILGRKMKDWLRFAVAWWHTLGQASGDQ
		Acid		FGGQTRNYAWDEPECPVERAKAKADAGFELMQKLGIEYFCFHDVDLIEEAATIEEYEERM
				GIITDYLLGKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNAIDATIK
				LGGENYVFWGGREGYASLLNTQMQREKDHLGRMLAAARDYGRAHGFKGTFLIEPKPMEPT
				KHQYDQDTETVIAFLRRNGLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGLLGSIDANR
				GDAQNGWDTDQFPIDNFELTQAMLQIIRNGGLGTGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALANAAAIIEESPIPAMLKERYASFDSGKGKEFEDGKLSLEELVAYAKANGEPK
				QISGKQELYETIVALYCK
5609MI1_005	Prevotella	DNA	147	ATGGCACAAGAATACTTCCCTACCATTGGGAAAATCCCCTTCGAGGGCACTGAGAGCAAG
				AATCCCCTTGCTTTCCATTACTATGAGCCGGAGCGCATTGTCTGCGGCAAACCCATGAAA
				GAATGGCTCAAGTTTGCCATGGCCTGGTGGCACACGCTGGGGCAGGCATCGGCCGATCCC
				TTCGGCGGCCAAACCCGCAGCTATGCCTGGGATAAGGGCGAATGCCCCTACTGCCGTGCC
				CGCGCCAAGGCGGACGCCGGCTTCGAGATTATGCAAAAGCTGGGCATCGAGTACTTCTGC
				TTCCACGATATCGACCTGGTAGAAGACTGTGACGATATTGCGGAATACGAAGCCCGCATG
				AAGGACATCACGGACTACCTCCTGGAGAAGATGAAGGAAACCGGTATCAAGAACCTCTGG
				GGCACCGCCAATGTGTTTGGTCACAAGCGCTACATGAACGGCGCCGCCACCAACCCGCAG
				TTTGACGTAGTGGCCCGTGCCGCTGTTCAGATTAAGAACGCCATTGACGCCACCATCAAG
				TTGGGCGGTGCCAATTACGTGTTCTGGGGCGGCCGCGAGGGCTATTACAGCCTCCTGAAC
				ACCCAGATGCAGCGGGAGAAGGACCACCTGGCCAAGCTGCTCACGGCAGCCCGCGACTAT
				GCCCGCGCCAACGGCTTCAAGGGAACCTTCCTGATTGAGCCCAAGCCCATGGAGCCCACC
				AAGCACCAGTACGACGTGGATACGGAGACGGTCATTGGCTTCCTCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAATATCGAGGTGAACCACGCCACGTTGGCCGGCCACACCTTT
				GAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCAGCATCGACGCCAACCGC
				GGCGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTAGACGCTTATGAGCTCACC
				CAGGCCATGATGCAGGTGCTCCTGAACGGAGGCTTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAGCTGCGCCGCTCCTCCACGGACCCGGAGGACATCTTCATCGCCCATATCAGTGCG
				ATGGATGCCATGGCCCACGCCCTGCTCAACGCCGCCGCCGTGCTGGAGGAAAGCCCCCTG
				TGCCAGATGGTGAAGGAGCGCTACGCCAGCTTTGACAGCGGTCCGGGCAAGCAGTTCGAG
				GAAGGAAAGGCCACCCTGGAGGACCTGTACAACTACGCCAAAGCCACCGGTGAACCCGTG
				GTTGCCTCCGGCAAGCAGGAACTTTACGAGACCCTCCTGAACCTCTATGCAAAGTAG
5609MI1_005	Prevotella	Amino	148	MAQEYFPTIGKIPFEGTESKNPLAFHYYEPERIVCGKPMKEWLKFAMAWWHTLGQASADP
		Acid		FGGQTRSYAWDKGECPYCRARAKADAGFEIMQKLGIEYFCFHDIDLVEDCDDIAEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDVVARAAVQIKNAIDATIK
				LGGANYVFWGGREGYYSLLNTQMQREKDHLAKLLTAARDYARANGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDAYELTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVLEESPLCQMVKERYASFDSGPGKQFEEGKATLEDLYNYAKATGEPV
				VASGKQELYETLLNLYAK
5610MI1_003	Prevotella	DNA	149	ATGGCACAAGAATACTTCCCTACCATTGGGAAAATCCCCTTCGAGGGCACTGAGAGCAAG
				AATCCCCTTGCTTTCCATTACTATGAGCCGGAGCGCATTGTCTGCGGCAAACCCATGAAA
				GAATGGCTCAAGTTTGCCATGGCCTGGTGGCACACGCTGGGGCAGGCATCGGCCGATCCC
				TTCGGCGGCCAAACCCGCAGCTATGCCTGGGATAAGGGCGAATGCCCCTACTGCCGTGCC
				CGTGCCAAGGCGGACGCCGGTTTTGAGATTATGCAAAAGCTGGGCATCGAGTACTTCTGC
				TTCCACGATATCGACCTGGTAGAAGACTGTGACGATATTGCGGAATACGAAGCCCGCATG
				AAGGACATCACGGACTACCTCCTGGAGAAGATGAAGGAAACCGGCATCAAGAACCTCTGG
				GGCACCGCCAATGTGTTTGGTCACAAGCGCTACATGAACGGCGCCGGCACCAATCCGCAG
				TTTGACGTGGTGGCCCGTGCTGCCGTGCAAATCAAGAACGCCATTGACGCCACCATCAAG
				TTGGGCGGTGCCAATTACGTGTTCTGGGGCGGCCGCGAGGGCTATTACAGCCTCCTGAAC
				ACCCAGATGCAGCGGGAGAAGGACCACCTGGCCAAGCTGCTCACGGCAGCCCGCGACTAT
				GCCCGCGCCAACGGCTTCAAGGGAACCTTCCTGATTGAGCCCAAGCCCATGGAGCCCACC
				AAGCACCAGTACGACGTGGATACGGAGACGGTCATTGGCTTCCTCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAATATCGAGGTGAACCACGCCACGCTGGCCGGCCACACCTTT
				GAGCACGAACTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCAGCATCGACGCCAACCGC
				GGCGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTAGACGCTTATGAGCTCACC
				CAGGCCATGATGCAGGTGCTCCTGAACGGAGGCTTCGGCAACGGCGGCACCAACTTCGAC
				GCCAAGCTGCGCCGCTCCTCCACGGACCTGGAGGACATCTTCATCGCCCATATCAGTGCG
				ATGGATGCCATGGCCCACGCCCTGCTCAACGCCGCCGCCGTGCTGGAGGAAAGCCCCCTG
				TGCCAGATGGTGAAGGAGCGCTACGCCAGCTTTGACAGCGGTCCGGGCAAGCAGTTCGAG
				GAAGGAAAGGCCACCCTGGAGGACCTGTACAACTACGCCAAAGCCAACGGTGAACCCGTG
				GTTGCCTCCGGCAAGCAGGAACTTTACGAGACCCTCCTGAACCTCTATGCAAAGTAG
5610MI1_003	Prevotella	Amino	150	MAQEYFPTIGKIPFEGTESKNPLAFHYYEPERIVCGKPMKEWLKFAMAWWHTLGQASADP
		Acid		FGGQTRSYAWDKGECPYCRARAKADAGFEIMQKLGIEYFCFHDIDLVEDCDDIAEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAGTNPQFDVVARAAVQIKNAIDATIK
				LGGANYVFWGGREGYYSLLNTQMQREKDHLAKLLTAARDYARANGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDAYELTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDLEDIFIAHISA
				MDAMAHALLNAAAVLEESPLCQMVKERYASFDSGPGKQFEEGKATLEDLYNYAKANGEPV
				VASGKQELYETLLNLYAK
5610MI2_004	Prevotella	DNA	151	ATGGCAAAAGAATATTTCCCTACCATCGGCAAGATTCCTTTTGAAGGAACCGACAGCAAG
				AGTCCCCTCGCCTTCCATTACTATGACGCCCAGCGCGTTGTGATGGGCAAACCCATGAAG
				GAATGGCTCAAGTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCATCGGCCGACCCC
				TTCGGCGGTCAGACCCGCCACTATGCCTGGGATGAAGGCGAATGCCCCTACTGCCGCGCC
				AAAGCCAAGGCCGACGCCGGCTTCGAGATCATGCAGAAACTGGGCATCGAGTACTTCTGC
				TTCCACGATGTGGACCTGGTGGAAGACTGCGACGACATCGCCGAGTACGAAGCCCGCATG
				AAGGACATCACGGACTACCTGCTGGAGAAGATGAAGGAAACCGGCATCAAGAACCTCTGG
				GGCACGGCCAATGTGTTCGGCCACAAGCGTTACATGAACGGCGCCGGGACCAACCCGCAG
				TTTGACATTGTGGCCCGCGCTGCCGTCCAGATCAAAAACGCCCTGGACGCCACCATCAAG
				CTGGGCGGTTCCAACTACGTGTTCTGGGGCAGCCGCGAAGGCTACTACACCCTCCTGAAC
				ACCCAGATGCAGCGGGAGAAAGACCACCTGGCCAAGCTCCTGACCGCCGCCCGCGACTAC
				GCCCGCGCCAAAGGCTTCAAGGGAACCTTCCTCATCGAGCCCAAACCCATGGAGCCCACC
				AAGCACCAGTACGACGTGGACACCGAGACCGTAATCGGCTTCCTGCGTGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCTGGCCACACCTTC
				GAGCACGAACTCACCGTCGCCCGTGAAAACGGCTTCCTCGGATCGATCGACGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCCGTAGACGCCTATGACCTCACC
				CAGGCCATGATGCAGGTGCTGCTGAACGGCGGTTTCGGCAATGGCGGTACCAACTTCGAC
				GCCAAGCTCCGCCGCTCCTCCACGGATCCGGAAGACATCTTCATCGCCCACATCAGCGCC
				ATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTGCTGGAAGAAAGCCCGCTT
				CCCGCCATGGCGAAAGAGCGCTACGCCTCCTTTGACAGCGGACTTGGCAAGAAGTTCGAA
				GAGGGAAAGGCCACCCTCGAAGAGCTGTACGACTATGCCAAGGCTAACGACGCCCCTGTC
				GCCGCCTCCGGCAAGCAGGAACTTTACGAAACCTTCTTGAACCTCTATGCAAAATAG
5610MI2_004	Prevotella	Amino	152	MAKEYFPTIGKIPFEGTDSKSPLAFHYYDAQRVVMGKPMKEWLKFAMAWWHTLGQASADP
		Acid		FGGQTRHYAWDEGECPYCRAKAKADAGFEIMQKLGIEYFCFHDVDLVEDCDDIAEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAGTNPQFDIVARAAVQIKNALDATIK
				LGGSNYVFWGSREGYYTLLNTQMQREKDHLAKLLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPVDAYDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVLEESPLPAMAKERYASFDSGLGKKFEEGKATLEELYDYAKANDAPV
				AASGKQELYETFLNLYAK
5751MI1_003	Prevotella	DNA	153	ATGGCAAAACAGTATTTTCCGCAAATCGGAAAGATTAAATTCGAAGGAACAGAGAGCAAG
				AATCCGCTTGCGTTCCATTATTATGACGCAAACAGGGTAGTCCTCGGAAAGGCAATGGAG
				GAGTGGCTCAAGTTCGCAATGGCTTGGTGGCATACTCTCGGACAGGCTTCCGGAGACCAG
				TTCGGCGGCCAGACCCGCAGCTACGAGTGGGATCTTGCAGCCACCCCCGAGCAGCGCGCA
				AAGGACAAGCTCGACGCCGGCTTCGAAATAATGGAGAAACTTGGAATCAAGTATTTCTGT
				TTCCACGATGTTGACCTTATCGAAGACAGCGACGATATTGCGACATATGAGGCTCGTCTC
				AAGGACCTTACAGACTACGCTGCAGAGCAGATGAAGCTCCACGACATCAAGCTCCTCTGG
				GGTACAGCGAATGTATTCGGCAACAAGCGCTACATGAACGGTGCGGCTACAAACCCTGAT
				TTCGATGTAGTTGCCCGCGCAGCCGTTCAGATTAAGAACGCTATCGACGCGACCATCAAG
				CTCGGTGGTACCAGCTATGTATTCTGGGGCGGTCGTGAGGGATATCAGAGCCTGCTCAAC
				ACTCAGATGCAGCGTGAGAAGGACCACCTCGCAACCATGCTTACAATCGCTCGCGACTAT
				GCTCGCAGCAAGGGCTTTACCGGAACCTTCCTTATCGAGCCTAAGCCGATGGAGCCTACA
				AAACACCAGTACGACGTAGATACAGAGACTGTTGTCGGCTTCCTCAAGGCACACGGCCTG
				GACAAGGACTTCAAGGTAAATATCGAGGTTAACCACGCAACTCTCGCAGGCCACACCTTC
				GAGCACGAACTCACCGTTGCTGTGGATAACGGAATGCTCGGTTCTATCGACGCTAACCGC
				GGTGATGCACAGAACGGCTGGGATACAGACCAGTTCCCTGTAAGCGCTGAGGAGCTTACC
				CTCGCTATGATGCAGATTATCCGTAATGGTGGCCTTGGCAACGGAGGATCCAACTTCGAC
				GCAAAGCTTCGCCGCAACTCTACCGATCCTGAAGACATCTTCATCGCACACATCTGCGGT
				ATGGATGCAATGGCACACGCTCTCCTCAATGCAGCTGCAATTATCGAGGAGTCTCCTATC
				CCTACAATGGTTAAGGAGCGTTACGCTTCCTTCGACAGCGGTATGGGTAAGGACTTCGAG
				GATGGAAAGCTTACCCTCGAGGATCTCTACAGCTACGGCGTGAAGAACGGAGAGCCAAAG
				CAGACCAGCGCAAAGCAGGAGCTCTATGAGACTCTCATGAATATCTATTGCAAGTAA
5751MI1_003	Prevotella	Amino	154	MAKQYFPQIGKIKFEGTESKNPLAFHYYDANRVVLGKAMEEWLKFAMAWWHTLGQASGDQ
		Acid		FGGQTRSYEWDLAATPEQRAKDKLDAGFEIMEKLGIKYFCFHDVDLIEDSDDIATYEARL
				KDLTDYAAEQMKLHDIKLLWGTANVFGNKRYMNGAATNPDFDVVARAAVQIKNAIDATIK
				LGGTSYVFWGGREGYQSLLNTQMQREKDHLATMLTIARDYARSKGFTGTFLIEPKPMEPT
				KHQYDVDTETVVGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGMLGSIDANR
				GDAQNGWDTDQFPVSAEELTLAMMQIIRNGGLGNGGSNFDAKLRRNSTDPEDIFIAHICG
				MDAMAHALLNAAAIIEESPIPTMVKERYASFDSGMGKDFEDGKLTLEDLYSYGVKNGEPK
				QTSAKQELYETLMNIYCK
5751MI2_003	Prevotella	DNA	155	ATGGCAAAAGAATTTTTTCCACAAGTAGGCAAGATTCCATTTGAGGGTCCTGAAAGTACT
				AACGTACTCGCATTCCACTACTATGATCCAGAACGCGAAGTTCTTGGTAAGAAAATGAAA
				GATTGGCTGAAGTATGCTATGGCTTGGTGGCACACACTCGGTCAGGCAAGTGGCGACCAA
				TTCGGTGGTCAAACTCGTTCGTATGAATGGGATGAAGCCGACGATGTTCTTCAACGCGCA
				AAGGATAAAATGGATGCTGGTTTTGAATTGATGACCAAACTTGGCATTGAATACTACTGC
				TTCCATGATGTCGACCTTATTGAAGAAGGTGCAACAATTGAAGAATATGAAGCTCGTATG
				CAAGCTATCACCGACTACGCATTAGAAAAACAAAAAGAAACCGGCATTAAGCTCCTTTGG
				GGTACTGCTAATGTGTTTGGTCATAAGCGTTATATGAATGGTGCGGCAACAAACCCTGAC
				TTTGATGTAGTGGCTCGCGCTGCTGTACAAATCAAGAACGCTATCGATGCAACTATCAAG
				CTTGGTGGTCAAAACTATGTATTCTGGGGTGGCCGCGAAGGTTATATGAGTTTGCTCAAC
				ACTCAAATGCAACGCGAAAAAGACCACTTGGCAAAGATGCTTACCGCAGCTCGCGACTAT
				GCTCGTGCTAAGGGCTTCAAGGGTACATTCCTCGTTGAACCTAAGCCTATGGAACCAACT
				AAGCATCAATATGATACCGATACAGAAACTGTGATTGGTTTCCTCCGTGCAAATGGTCTT
				GAAAAAGACTTCAAGGTGAACATTGAAGTGAACCATGCTACTCTCGCTCAGCACACTTTC
				GAACACGAACTCGCTGTGGCTGTCGACAATGGCATGCTCGGTTCTATCGACGCTAACCGT
				GGCGATGCTCAAAATGGCTGGGATACCGACCAATTCCCAATCGACAACTACGAACTCACC
				CTCGCTATGCTCCAAATCATTCGCAATGGTGGTCTTGGCAATGGCGGTAGCAACCTCGAC
				GCTAAGATTCGTCGTAATAGCACCGACCTTGAAGACCTCTTTATCGCTCACATCAGTGGT
				ATGGATGCTATGGCTCGTGCACTTCTCAATGCTGCTGCAATCGTTGAAAAGAGCGAAATT
				CCTGCTATGTTGAAGCAGCGTTATGCAAGCTCTGATGCAGGTATGGGTAAGGACTTCGAA
				GAAGGAAAACTCACTCTCGAACAACTCGTAGACTATGCTAAGGCTAACGGCGAACCTGCT
				ACAGTAAGCGGCAAGCAAGAAAAGTATGAAACTCTCGTTGCTCTCTACGCTAAGTAA
5751MI2_003	Prevotella	Amino	156	MAKEFFPQVGKIPFEGPESTNVLAFHYYDPEREVLGKKMKDWLKYAMAWWHTLGQASGDQ
		Acid		FGGQTRSYEWDEADDVLQRAKDKMDAGFELMTKLGIEYYCFHDVDLIEEGATIEEYEARM
				QAITDYALEKQKETGIKLLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNAIDATIK
				LGGQNYVFWGGREGYMSLLNTQMQREKDHLAKMLTAARDYARAKGFKGTFLVEPKPMEPT
				KHQYDTDTETVIGFLRANGLEKDFKVNIEVNHATLAQHTFEHELAVAVDNGMLGSIDANR
				GDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLGNGGSNLDAKIRRNSTDLEDLFIAHISG
				MDAMARALLNAAAIVEKSEIPAMLKQRYASSDAGMGKDFEEGKLTLEQLVDYAKANGEPA
				TVSGKQEKYETLVALYAK
5752MI1_003	Prevotella	DNA	157	ATGACTAAAGAGTATTTCCCGGGAATCGGAAAGATTCCGTTTGAAGGAACCAAGAGCAAG
				AACCCCCTGGCCTTCCATTATTATAACGCCTCCCAGGTAGCGATGGGCAAGCCCATGAAG
				GACTGGCTCAAGTATGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCTGCAGACCCC
				TTTGGCGGCCAGACCCGCTCCTACGAATGGGACAAGGGCGAGTGCCCTTATTGCCGCGCC
				AAGCAGAAGGCCGATGCCGGCTTTGAGCTCATGCAGAAGCTGGGCATCGAGTACTACTGC
				TTCCACGACGTGGACATCATCGAGGACTGCGAGGACATTGCCGAGTACGAGGCCCGCATG
				AAGGACATCACGGACTACCTGCTGGAGAAGCAGAAAGAGACCGGCATCAAGAACCTCTGG
				GGCACCGCCAACGTGTTTGGCCACAAGCGCTACATGAACGGCGCCGCCACCAACCCTCAG
				TTTGACATTGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGATGCCACCATCAAG
				CTGGGTGGTACCAACTACGTGTTCTGGGGTGGCCGCGAAGGCTACTACACGCTGCTCAAC
				ACCCAGATGCAGCGGGAGAAGAACCACCTGGCCAAGATGCTCACCGCCGCCCGCGACTAC
				GCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAGCCCACC
				AAGCACCAGTACGACGTGGACACCGAGACCGTGATTGGTTTCATCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTAAACATTGAGGTAAACCACGCCACCCTGGCCGGCCACACCTTT
				GAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCTCCATCGACGCCAACCGC
				GGAGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCCATCGACGCCCTGGATCTCACC
				CAGGCTATGATGCAGGTCATCCTCAACGGTGGCTTCGGCAATGGCGGCACCAACTTTGAC
				GCCAAGCTCCGCCGCTCCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCC
				ATGGATGCCATGGCACACGCCCTCCTGAACGCAGCCGCCATCCTGGAAGAGAGCCCCCTG
				CCCGCCATGGTCAAGGAGCGTTACGCTTCCTTCGACAGCGGTCTGGGCAAGAAGTTCGAA
				GAAGGCAAGGCCTCCCTGGAAGAACTTTACGAATATGCCAAGAAGAATGGAGAGCCCGTG
				GCCGCTTCCGGCAAACAGGAGCTCTGCGAAACTTACTTGAACCTCTATGCAAAGTAG
5752MI1_003	Prevotella	Amino	158	MTKEYFPGIGKIPFEGTKSKNPLAFHYYNASQVAMGKPMKDWLKYAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKGECPYCRAKQKADAGFELMQKLGIEYYCFHDVDIIEDCEDIAEYEARM
				KDITDYLLEKQKETGIKNLWGTANVFGHKRYMNGAATNPQFDIVARAAVQIKNALDATIK
				LGGTNYVFWGGREGYYTLLNTQMQREKNHLAKMLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFIRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPIDALDLTQAMMQVILNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPLPAMVKERYASFDSGLGKKFEEGKASLEELYEYAKKNGEPV
				AASGKQELCETYLNLYAK
5752MI2_003	Prevotella	DNA	159	ATGACTAAAGAGTATTTCCCGGGAATCGGAAAGATTCCGTTTGAAGGAACCAAGAGCAAG
				AACCCCCTGGCCTTCCATTATTATAACGCCTCCCAGGTAGTGATGGGCAAGCCCATGAAG
				GACTGGCTCAAGTATGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCTGCAGACCCC
				TTTGGCGGCCAGACCCGCTCCTACGAATGGGACAAGGGCGAGTGCCCGTACTGCCGCGCC
				AAGCAGAAGGCCGATGCCGGCTTTGAGCTCATGCAGAAGCTGGGCATCGAGTACTACTGC
				TTCCACGACGTGGACATCATCGAGGACTGCGAGGACATTGCCGAGTACGAGGCCCGCATG
				AAGGACATCACGGACTACCTGCTGGAGAAGCAGAAAGAGACCGGCATCAAGAACCTCTGG
				GGCACCGCCAACGTGTTTGGCCACAAGCGCTACATGAACGGCGCCGCCACCAACCCTCAG
				TTTGACATTGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGATGCCACCATCAAA
				CTGGGTGGTACCAACTACGTGTTCTGGGGTGGCCGCGAAGGCTACTACACGCTGCTCAAC
				ACCCAGATGCAGCGGGAGAAGAACCACCTGGCCAAGATGCTCACCGCCGCCCGCGACTAC
				GCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAGCCCACC
				AAGCACCAGTACGACGTGGACACCGAGACCGTGATTGGTTTCATCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTAAACATTGAGGTAAACCACGCCACCCTGGCCGGCCACACCTTT
				GAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCTCCATCGACGCCAACCGC
				GGAGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCCATCGACGCCCTGGATCTCACC
				CAGGCTATGATGCAGGTCATCCTCAACGGTGGCTTCGGCAATGGCGGCACCAACTTTGAC
				GCCAAGCTCCGCCGCTCCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCC
				ATGGATGCCATGGCACACGCCCTCCTGAACGCAGCCGCCATCCTGGAAGAGAGCCCCCTG
				CCCGCCATGGTCAAGGAGCGTTACGCTTCCTTCGACAGCGGTCTGGGCAAGAAGTTCGAA
				GAAGGCAAGGCCTCCCTGGAAGAACTTTACGAATATGCCAAGAAGAATGGAGAGCCCGTG
				GCCGCTTCCGGCAAACAGGAGCTCTGCGAAACTTACTTGAACCTCTATGCAAAGTAG
5752MI2_003	Prevotella	Amino	160	MTKEYFPGIGKIPFEGTKSKNPLAFHYYNASQVVMGKPMKDWLKYAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKGECPYCRAKQKADAGFELMQKLGIEYYCFHDVDIIEDCEDIAEYEARM
				KDITDYLLEKQKETGIKNLWGTANVFGHKRYMNGAATNPQFDIVARAAVQIKNALDATIK
				LGGTNYVFWGGREGYYTLLNTQMQREKNHLAKMLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFIRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPIDALDLTQAMMQVILNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPLPAMVKERYASFDSGLGKKFEEGKASLEELYEYAKKNGEPV
				AASGKQELCETYLNLYAK
5752MI3_002	Prevotella	DNA	161	ATGGCAAAAGAGTATTTCCCGACTATCGGCAAGATTCCCTTCGAGGGCGTCGAATCCAAG
				AACCCGATGGCATTCCACTACTATGACGCGAACCGCGTCGTGATGGGCAAGCCCATGAAG
				GACTGGCTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGACAGGCTTCCGGCGACCCG
				TTCGGCGGCCAGACCCGTTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCC
				AAGGCCAAGGCCGACGCCGGCTTCGAGATCATGCAGAAGCTCGGTATCGAGTACTACTGC
				TTCCATGACATCGACCTCGTGGAGGACACCGAGGACATCGCCGAGTACGAGGCCCGCATG
				AAGGACATCACCGACTACCTCGTCGAGAAGCAGAAGGAAACCGGCATCAAGAACCTCTGG
				GGCACGGCCAACGTGTTCGGCAACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAG
				TTCGACGTCGTCGCCCGCGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTCGGCGGTACCGGTTACGTGTTCTGGGGCGGCCGTGAAGGCTACTACACCCTCCTGAAC
				ACCCAGATGCAGCGCGAGAAGGACCACCTCGCCAAGATGCTCACCGCCGCCCGCGACTAC
				GCCCGCGCCCACGGCTTCCAGGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCCACC
				AAGCACCAGTACGACGTGGACACGGAGACCGTGATCGGCTTCCTGCGCGCCAACGGTCTG
				GACAAGGACTTCAAGGTCAATATCGAGGTGAACCACGCCACCCTCGCCGGCCACACCTTC
				GAGCACGAGCTCACCGTGGCTGTCGATAACGGCTTCCTCGGCTCCATCGACGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCCGTGGACCCGTACGACCTCACC
				CAGGCCATGATGCAGATCATCCGCAACGGCGGTTTCAAGGACGGCGGCACCAACTTCGAC
				GCCAAGCTCCGCCGCTCTTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTCATCGAGGAGAGCCCGCTC
				TGCAAGATGGTCGAGGAGCGCTACGCTTCCTTCGACAGCGGCCTCGGCAAGCAGTTCGAG
				GAAGGCAAGGCCACCCTCGAGGACCTCTACGAGTATGCCAAGAAGAATGGCGAGCCCGTC
				GTCGCCTCCGGCAAGCAGGAGCTCTACGAGACGCTGCTGAACCTTTACGCGAAGTAG
5752MI3_002	Prevotella	Amino	162	MAKEYFPTIGKIPFEGVESKNPMAFHYYDANRVVMGKPMKDWLKFAMAWWHTLGQASGDP
		Acid		FGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYYCFHDIDLVEDTEDIAEYEARM
				KDITDYLVEKQKETGIKNLWGTANVFGNKRYMNGAATNPQEDVVARAAVQIKNAIDATIK
				LGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAHGFQGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANR
				GDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVIEESPLCKMVEERYASFDSGLGKQFEEGKATLEDLYEYAKKNGEPV
				VASGKQELYETLLNLYAK
5752MI5_003	Prevotella	DNA	163	ATGGCAAAAGAGTATTTCCCGACAATCGGTAAGATCCCCTTCGAGGGACCCGAGTCCAAG
				AACCCGATGGCATTCCACTACTATGACGCGGAGCGCGTGGTGATGGGCAAGAAGATGAAG
				GACTGGTTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGCCAGGCTTCCGCCGACCCG
				TTCGGCGGCCAGACCCGCTCCTACGAGTGGGACAAGGGCGAAGGCCCCTGCTCCCGCGCC
				CGCGCCAAGGCTGACGCCGGTTTCGAGATCATGCAGAAACTGGGCATCGGCTACTACTGC
				TTCCACGACATCGACCTGGTGGAGGACACCGAGGACATCGCCGAGTATGAAGCCCGCATG
				AAGGACATCACCGACTACCTCGTGGAGAAGCAGAAGGAGACCGGCATCAAGAACCTCTGG
				GGCACGGCCAACGTATTCGGCAACAAGCCCTACATGAACGGCGCCGCCACGAACCCGCAG
				TTCGACATCGCCGCCCGCGCGGCCCTGCAGACCAAGAACGCCATCGATGCCACCATCAAG
				CTGGGCGGCACCGGTTACGTGTTCTGGGGCGGCCGTGAAGGCTACTACACCCTCCTGAAC
				ACCCAGATGCAGCGCGAGAAGGACCACCTTGCCAAGATGCTCACCGCGGCTCGCGACTAT
				GCCCGCGCCCACGGCTTCAAGGGCACCTTCTTCATCGAGCCGAAACCGATGGAGCCCACC
				AAGCACCAGTACGACGTGGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAACATCGAAGTGAACCACGCCACCCTCGCCGGCCACACCTTC
				GAGCACGGGCTCACCGTGGCCGTTGACAACGGCTTCCTCGGCAGCATCGACGCCAACCGC
				GGAGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGGTGGATCCGTACGACCTCACC
				CAGGCGATGATCCAGATCATCCGCAATGGCGGCTTCAAGGACGGCGGTACCAACTTCGAC
				GCCAAGCTCCGCCGCTCTTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTGCTCGAGGAGAGCCCGCTC
				TGCGAGATGGTTGCAAAGCGTTACGCTTCCTTCGACAGCGGTCTCGGCAAGAAGTTCGAG
				GAAGGCAACGCCACCCTCGAGGAACTCTACGAGTACGCCAAGGCGAAGGGCGAGGTCGTT
				GCCGAATCCGGCAAGCAGGAACTCTACGAGACCCTGCTGAACCTCTACGCGAAGTAG
5752MI5_003	Prevotella	Amino	164	MAKEYFPTIGKIPFEGPESKNPMAFHYYDAERVVMGKKMKDWFKFAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKGEGPCSRARAKADAGFEIMQKLGIGYYCFHDIDLVEDTEDIAEYEARM
				KDITDYLVEKQKETGIKNLWGTANVFGNKPYMNGAATNPQFDIAARAALQTKNAIDATIK
				LGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAHGFKGTFFIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHGLTVAVDNGFLGSIDANR
				GDAQNGWDTDQFPVDPYDLTQAMIQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVLEESPLCEMVAKRYASFDSGLGKKFEEGNATLEELYEYAKAKGEVV
				AESGKQELYETLLNLYAK
5752MI_6004	Prevotella	DNA	165	ATGGCAAAAGAGTATTTCCCGACAATCGGAAAGATCCCCTTCGAGGGCGCTGAGAGCAAG
				AATCCCCTTGCTTTCCACTATTATGACGCCGAGCGTGTGGTCATGGGCAAGCCCATGAAG
				GACTGGTTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGCCAGGCTTCCGCCGACCCG
				TTCGGCGGCCAGACCCGCTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCC
				CGCCAGAAGGCTGACGCCGGTTTCGAGATCATGCAGAAGCTCGGCATCGGCTACTACTGC
				TTCCACGACATCGACCTGGTCGAGGACACCGAGGACATCGCCGAGTACGAGGCCCGCATG
				AAGGACATCACCGACTACCTCGTCGAGAAGCAGAAGGAGACCGGCATCAAGAACCTCTGG
				GGCACGGCCAACGTGTTCGGCAACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAG
				TTCGACATCGTCGCCCACGCGGCCCTGCAGATCAAGAACGCGATCGGCGCCACCATCAAG
				CTCGGCGGCACCGGTTACGTGTTCTGGGGCGGCCGTGAAGGTTACTACACCCTCCTGAAC
				ACCCAGATGCAGCGCGAGAAGGACCACCTCGCCAAGATGCTCACCGCCGCCCGCGACTAC
				GCCCGCGCCAACGGCTTCAAGGGCACCTTCCTCATCGAGCCGAAGCCGATGGAGCCCACC
				AAGCACCAGTATGACGTGGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTCGCCGGCCACACCTTC
				GAGCACGAGCTCACCGTGGCGGTCGACAACGGCTTCCTCGGCAGCATCGACGCCAACCGC
				GGTGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGGTGGATCCGTACGATCTCACC
				CAGGCGATGATCCAGATCATCCGCAACGGCGGCTTCAAGGATGGCGGCACCAACTTCGAC
				GCCAAGCTCCGCCGCTCTTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTCATCGAGGAGAGCCCGCTC
				TGCGAGATGGTCGCCAAGCGCTACGCTTCCTTCGACAGCGGTCTCGGCAAGAAGTTCGAG
				GAAGGCAACGCCACCCTCGAGGAACTCTACGAGTACGCCAAGGCGAACGGTGAGGTCAAG
				GCCGAATCCGGCAAGCAGGAGCTCTACGAGACCCTTCTGAACCTCTACGCGAAATAG
5752MI6_004	Prevotella	Amino	166	MAKEYFPTIGKIPFEGAESKNPLAFHYYDAERVVMGKPMKDWFKFAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKGECPYCRARQKADAGFEIMQKLGIGYYCFHDIDLVEDTEDIAEYEARM
				KDITDYLVEKQKETGIKNLWGTANVEGNKRYMNGAATNPQFDIVAHAALQIKNAIGATIK
				LGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARANGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANR
				GDAQNGWDTDQFPVDPYDLTQAMIQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVIEESPLCEMVAKRYASFDSGLGKKFEEGNATLEELYEYAKANGEVK
				AESGKQELYETLLNLYAK
5753MI1_002	Prevotella	DNA	167	ATGGCAAAAGAGTATTTCCCCACTATCGGGAAGATTCCTTTCGAAGGAGTCGAGAGCAAG
				AACCCCCTTGCATTCCATTATTATGACGCAAACCGCATGGTCATGGGCAAGCCCATGAAG
				GACTGGTTCAAGTTCGCCATGGCATGGTGGCACACCCTGGGACAGGCCTCCGCAGACCCG
				TTCGGCGGCCAGACCCGCTCCTACGAATGGGACAAGGGCGAATGCCCCTACTGCCGCGCC
				AGGGCAAAGGCCGATGCCGGCTTCGAGATCATGCAGAAACTGGGTATCGAGTATTTCTGC
				TTCCATGACATCGACCTGGTAGAGGACTGCGACGACATCGCCGAGTACGAGGCCCGCATG
				AAGGACATCACGGACTATCTCCTGGAGAAGATGAAGGAAACCGGCATCAAGAACCTCTGG
				GGCACCGCCAACGTGTTCGGCAACAAGCGTTACATGAACGGCGCCGGCACCAATCCGCAG
				TTCGACGTAGTGGCCCGCGCTGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAG
				CTCGGCGGTTCCAACTATGTGTTCTGGGGCGGCCGTGAAGGATACTACACCCTGCTGAAC
				ACCCAGATGCAGCGCGAGAAGGACCACCTCGGCAAACTGCTCACCGCCGCCCGCGACTAT
				GCCCGCAAGAACGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCGATGGAGCCCACC
				AAGCACCAGTACGACGTAGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTG
				GAGAAAGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCATACCTTC
				GAGCATGAACTCACCGTGGCCGTGGACAACGGCTTCCTGGGATCCATCGACGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTAGACCCGTACGACCTCACC
				CAGGCCATGATGCAGATCATCCGCAACGGCGGCCTCGGCAACGGCGGTACCAACTTCGAC
				GCCAAACTGCGCCGTTCCTCCACCGATCCTGAGGACATCTTCATCGCCCACATCAGCGCC
				ATGGACGCCATGGCCCACGCCCTGCTCAACGCAGCCGCCGTGCTGGAAGAAAGTCCGCTC
				TGTGAGATGGTCAAGGAGCGCTACGCTTCCTTCGACAGCGGTCTCGGCAAGAAGTTCGAA
				GAGGGCAAGGCTACCCTGGAAGAAATCTACGAGTATGCCAAGAAGAGCGGCGAACCCGTG
				GTCGCTTCCGGCAAGCAGGAGCTCTACGAAACCCTGCTGAACCTCTACGCCAAGTAG
5753MI1_002	Prevotella	Amino	168	MAKEYFPTIGKIPFEGVESKNPLAFHYYDANRMVMGKPMKDWFKFAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKGECPYCRARAKADAGFEIMQKLGIEYFCFHDIDLVEDCDDIAEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGNKRYMNGAGTNPQEDVVARAAVQIKNAIDATIK
				LGGSNYVFWGGREGYYTLLNTQMQREKDHLGKLLTAARDYARKNGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFLRANGLEKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANR
				GDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGLGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVLEESPLCEMVKERYASFDSGLGKKFEEGKATLEEIYEYAKKSGEPV
				VASGKQELYETLLNLYAK
5753MI2_002	Prevotella	DNA	169	ATGGCTAAAGAATACTTCCCCTCCATCGGCAAAATCCCTTTTGAAGGAGGCGACAGCAAA
				AATCCCCTCGCTTTCCATTATTATGACGCCGGACGCGTGGTTATGGGCAAGCCCATGAAG
				GAATGGCTTAAATTCGCCATGGCCTGGTGGCACACGCTGGGCCAGGCCTCCGGAGACCCC
				TTCGGCGGCCAGACCCGCAGCTACGAATGGGACAAGGGCGAATGCCCCTACTGCCGCGCC
				AAAGCCAAGGCCGACGCCGGTTTTGAAATCATGCAAAAGCTGGGTATCGAATACTTCTGC
				TTCCACGATGTGGACCTTATCGAGGATTGCGATGACATTGCCGAATACGAAGCCCGCATG
				AAGGACATCACGGACTACCTGCTGGAAAAGATGAAGGAGACCGGCATCAAGAACCTCTGG
				GGCACCGCCAATGTCTTCGGCCACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAG
				TTCGACGTGGTCGCCCGCGCCGCCGTCCAGATCAAGAACGCGATTGACGCCACCATCAAG
				CTCGGCGGTACCAGTTATGTATTCTGGGGCGGCCGCGAGGGCTACTACACCCTCCTGAAC
				ACCCAGATGCAGCGTGAGAAAGACCACCTGGCCAAGATGCTCACCGCAGCCCGCGACTAC
				GCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCGATGGAGCCCACC
				AAGCACCAGTACGACGTTGACACGGAGACCGTGATCGGCTCCCTGCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTC
				GAGCACGAACTCACCGTGGCTGTTGACAACGGCTTCCTGGGCTCCATCGACGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTAGACCCGTACGACCTCACC
				CAGGCCATGATGCAGATTATCCGCAACGGCGGCTTCAAGGACGGCGGCACCAACTTCGAT
				GCCAAACTGCGCCGCTCTTCCACCGATCCGGAAGACATCTTCATCGCCCACATCAGCGCT
				ATGGATGCCATGGCACACGCCCTGCTCAACGCCGCCGCCGTGCTGGAAGAGAGCCCGCTG
				TGCAACATGGTCAAGGAGCGTTACGCCGGCTTCGACAGCGGCCTTGGCAAGAAGTTCGAG
				GAAGGGAAGGCAACGCTGGAGGAAATCTATGACTATGCCAAGAAGAGCGGCGAACCCGTC
				GTGGCTTCCGGCAAGCAGGAACTCTACGAAACCATCCTGAACCTCTATGCCAAGTAG
5753MI2_002	Prevotella	Amino	170	MAKEYFPSIGKIPFEGGDSKNPLAFHYYDAGRVVMGKPMKEWLKFAMAWWHTLGQASGDP
		Acid		FGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYFCFHDVDLIEDCDDIAEYEARM
				KDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDVVARAAVQIKNAIDATIK
				LGGTSYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGSLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANR
				GDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAVLEESPLCNMVKERYAGFDSGLGKKFEEGKATLEEIYDYAKKSGEPV
				VASGKQELYETILNLYAK
5753MI4_002	Prevotella	DNA	171	ATGTCAAAAGAGTATTTCCCTACAATCGGCAGGGTCCCCTTCGAGGGACCTGAGAGCAAG
				AATCCGCTGGCGTTCCACTATTACGAGCCGGACCGGCTCGTCCTGGGCAGGAAAATGAAG
				GACTGGCTGCGCTTCGCAATGGCCTGGTGGCATACGCTCGGGCAGGCTTCCGGCGACCAG
				TTCGGCGGACAGACCTGCACATACGCCTGGGATGAAGGCGAGTGTCCCGTCTGCCGGGCA
				AAGGCCAAGGCTGACGCCGGCTTTGAACTGATGCAGAAACTGGGCATCGGGTATTTCTGC
				TTCCACGACGTGGACCTGGTCGAGGAGGCCGACACCATTGAAGAATACGAGGAGCGGATG
				CGGATCATCACCGACTACCTGCTCGAGAAGATGGAAGAGACCGGCATCCGCAATCTCTGG
				GGAACCGCCAATGTCTTCGGACACAAGCGCTATATGAACGGCGCCGCCACCAATCCCGAC
				TTCGACGTCGTGGCCCGTGCCGCGGTCCAGATCAAGAATGCCATCGATGCCACCATCAAA
				CTGGGTGGTGAGAACTATGTGTTCTGGGGTGGCCGCGAGGGCTATACGAGCCTGCTCAAC
				ACGCAGATGCACCGGGAAAAACACCACCTCGGAAATATGCTCAGGGCAGCCCGCGACTAT
				GGCCGTGCCCACGGTTTCAAGGGAACGTTCCTGATCGAGCCCAAGCCGATGGAGCCGACC
				AAGCATCAGTACGACCAGGATACGGAGACGGTCATCGGTTTCCTGCGCTGTCACGGCCTG
				GACAAGGATTTCAAGGTGAACATCGAGGTGAACCACGCCACGCTCGCCGGACACACCTTC
				GAGCACGAACTGGCCACTGCGGTCGATGCCGGCCTGCTGGGCAGCATCGATGCCAACCGC
				GGCGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGATCGACAACTACGAACTCACG
				CTGGCGATGCTGCAGATCATCCGCAATGGCGGACTCGCACCCGGCGGATCGAACTTCGAT
				GCCAAGTTGCGCCGCAATTCCACCGATCCGGAAGACATCTTCATCGCCCACATCAGCGCG
				ATGGACGCGATGGCCCGTGCCCTGCTCAATGCGGCGGCCATCTGGACCGAATCGCCGATT
				CAGGATATGGTCAGGGACCGCTATGCTTCCTTCGACAGCGGAAAGGGCAGGGAGTTCGAG
				GAAGGCAGACTCAGTCTGGAAGACCTCGTGGCCTATGCGAAGGAGCACGGTGAGCCGCGC
				CAGATCTCCGGCAGGCAGGAACTTTATGAAACCATCGTAGCGCTTTACTGCAGGTAA
5753MI4_002	Prevotella	Amino	172	MSKEYFPTIGRVPFEGPESKNPLAFHYYEPDRLVLGRKMKDWLRFAMAWWHTLGQASGDQ
		Acid		FGGQTCTYAWDEGECPVCRAKAKADAGFELMQKLGIGYFCFHDVDLVEEADTIEEYEERM
				RIITDYLLEKMEETGIRNLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNAIDATIK
				LGGENYVFWGGREGYTSLLNTQMHREKHHLGNMLRAARDYGRAHGFKGTFLIEPKPMEPT
				KHQYDQDTETVIGFLRCHGLDKDFKVNIEVNHATLAGHTFEHELATAVDAGLLGSIDANR
				GDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISA
				MDAMARALLNAAAIWTESPIQDMVRDRYASFDSGKGREFEEGRLSLEDLVAYAKEHGEPR
				QISGRQELYETIVALYCR
5752MI4_004	Prevotella	DNA	173	ATGACTAAAGAGTATTTCCCGGGAATCGGAACGATTCCGTTTGAAGGAACCAAGAGCAAG
				AACCCCCTGGCCTTCCATTATTATAACGCCTCCCAGGTAGTGATGGGCAAGCCCATGAAG
				GACTGGCTCAAGTATGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCTGCAGACCCC
				TTTGGCGGCCAGACCCGCTCCTACGAATGGGACAAGGGCGAGTGCCCGTACTGCCGCGCC
				AAGCAGAAGGCCGATGCCGGCTTTGAGCTCATGCAGAAGCTGGGCATCGAGTACTACTGC
				TTCCACGACGTGGACATCATCGAGGACTGCGAGGACATTGCCGAGTACGAGGCCCGCATG
				AAGGACATCACGGACTACCTGCTGGAGAAGCAGAAAGAGACCGGCATCAAGAACCTCTGG
				GGCACCGCCAACGTGTTTGGCCACAAGCGCTACATGAACGGCGCCGCCACCAACCCTCAG
				TTTGACATTGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGATGCCGCCATCAAA
				CTGGGTGGTACCAACTACGTGTTCTGGGGTGGCCGCGAAGGCTACTACACGCTGCTCAAC
				ACCCAGATGCAGCGGGAGAAGAACCACCTGGCCAAGATGCTCACCGCCGCCCGCGACTAC
				GCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAGCCCACC
				AAGCACCAGTACGACGTGGACACCGAGACCGTGATTGGTTTCATCCGCGCCAACGGCCTG
				GACAAGGACTTCAAGGTAAACATTGAGGTAAACCACGCCACCCTGGCCGGCCACACCTTT
				GAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCTCCATCGACGCCAACCGC
				GGAGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCCATCGACGCCCTGGATCTCACC
				CAGGCTATGATGCAGGTCATCCTCAACGGTGGCTTCGGCAATGGCGGCACCAACTTTGAC
				GCCAAGCTCCGCCGCTCCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCC
				ATGGATGCCATGGCACACGCCCTCCTGAACGCAGCCGCCATCCTGGAAGAGAGCCCCCTG
				CCCGCCATGGTCAAGGAGCGTTACGCTTCCTTCGACAGCGGTCTGGGCAAGAAGTTCGAA
				GAAGGCAAGGCCTCCCTGGAAGAACTTTACGAATATGCCAAGAAGAATGGAGAGCCCGTG
				GCCGCTTCCGGCAAACAGGAGCTCTGCGAAACTTACTTGAACCTCTATGCAAAGTAG
5752MI4_004	Prevotella	Amino	174	MTKEYFPGIGTIPFEGTKSKNPLAFHYYNASQVVMGKPMKDWLKYAMAWWHTLGQASADP
		Acid		FGGQTRSYEWDKGECPYCRAKQKADAGFELMQKLGIEYYCFHDVDIIEDCEDIAEYEARM
				KDITDYLLEKQKETGIKNLWGTANVFGHKRYMNGAATNPQFDIVARAAVQIKNALDAAIK
				LGGTNYVFWGGREGYYTLLNTQMQREKNHLAKMLTAARDYARAKGFKGTFLIEPKPMEPT
				KHQYDVDTETVIGFIRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANR
				GDAQNGWDTDQFPIDALDLTQAMMQVILNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISA
				MDAMAHALLNAAAILEESPLPAMVKERYASFDSGLGKKFEEGKASLEELYEYAKKNGEPV
				AASGKQELCETYLNLYAK
727MI4_006	Rhizobiales	DNA	175	GTGACTGATTTCTTCAAGGGCATCGCGCCCGTCAAGTTTGAGGGGCCGCAGAGCTCCAAT
				CCGCTGGCCTATCGCCACTATAACAAGGACGAAATCGTCCTCGGCAAGCGGATGGAAGAC
				CATATCCGTCCCGGCGTTGCCTATTGGCACACCTTCGCCTATGAGGGCGGCGATCCGTTT
				GGCGGCCGCACCTTCGATCGCCCCTGGTTCGACAAGGGTATGGACGGCGCCCGCCTCAAG
				GCCGACGTGGCCTTCGAACTGTTCGACCTGCTCGACGTTCCTTTCTTCTGTTTCCACGAT
				GCTGATATCGCTCCCGAAGGCGCAACGCTGGCCGAGAGCAACCGCAATGTGCGCGAGATT
				GGCGAGATCTTCGCTCGCAAGATGGAAACCAGCCGCACCAAGCTGCTCTGGGGTACGGCA
				AACCTGTTCTCCAATCGCCGCTACATGGCCGGCGCCGCCACCAACCCGGACCCGGAAATC
				TTCGCCTATGCCGCTGGGCAGGTGAAGAACGTGCTGGAACTGACCCACGAACTGGGCGGC
				GCCAACTATGTGCTGTGGGGCGGTCGCGAGGGTTATGAAACCCTGCTCAACACCAAGATC
				GGCCAGGAAATGGACCAGATGGGCCGTTTTCTGTCGATGGTCGTCGAGCATGCCGAAAAG
				ATCGGCTTCAAGGGCCAGATCCTGATCGAGCCCAAGCCGCAGGAGCCGAGCAAGCACCAG
				TATGACTTCGACGTTGCAACCGTTTACGGCTTCCTCAAGAAGTATGGTCTCGAAACCAAG
				GTGAAGTGCAATATCGAGGTCGGCCATGCCTTCCTCGCCAATCACTCCTTCGAGCATGAA
				CTGGCTTTGGCCGCATCGCTGGGCATTCTCGGCTCGGTCGACGCCAATCGCAACGATCTA
				CAGTCCGGCTGGGATACCGACCAGTTCCCCAATAATGTCCCCGAAACCGCACTCGCCTTC
				TATCAGATTCTCAAGGCGGGCGGACTGGGCAATGGCGGCTGGAACTTCGACGCCCGCGTG
				CGCCGCCAGTCACTTGATCCGGCCGACCTGCTGCACGGCCATATCGGCGGCCTCGACGTG
				CTGGCGCGCGGCCTCAAGGCCGCCGCGGCGCTGATCGAGGACGGCACCTATGACAAGGTC
				GTCGACGCCCGCTATGCCGGCTGGAACCAGGGCCTGGGCAAGGATATCCTTGGTGGCAAG
				CTGAACCTTGCCGACCTGGCTGCCAAGGTCGACGCCGAAAACCTCAACCCGCAGCCTAGG
				TCCGGCCAGCAGGAATATCTCGAAAACCTGATCAACCGGTTCGTTTAG
727MI4_006	Rhizobiales	Amino	176	MTDFFKGIAPVKFEGPQSSNPLAYRHYNKDEIVLGKRMEDHIRPGVAYWHTFAYEGGDPF
		Acid		GGRTFDRPWFDKGMDGARLKADVAFELFDLLDVPFFCFHDADIAPEGATLAESNRNVREI
				GEIFARKMETSRTKLLWGTANLFSNRRYMAGAATNPDPEIFAYAAGQVKNVLELTHELGG
				ANYVLWGGREGYETLLNTKIGQEMDQMGRELSMVVEHAEKIGFKGQILIEPKPQEPSKHQ
				YDFDVATVYGFLKKYGLETKVKCNIEVGHAFLANHSFEHELALAASLGILGSVDANRNDL
				QSGWDTDQFPNNVPETALAFYQILKAGGLGNGGWNFDARVRRQSLDPADLLHGHIGGLDV
				LARGLKAAAALIEDGTYDKVVDARYAGWNQGLGKDILGGKLNLADLAAKVDAENLNPQPR
				SGQQEYLENLINRFV

5.5 Example 4: Quantification of XI Enzyme Activity

The clones identified in the ABD and SBD screens (see Table 2) were subcloned into vector p426PGK1 (FIG. 3), a modified version of p426GPD (ATCC accession number 87361) in which the GPD promoter was replaced with the PGK1 promoter from Saccharomyces cerevisiae (ATCC accession number 204501) gDNA. The clones were then transformed into yeast strain MYA11008.

Cells were grown as described in the materials and methods. Cell pellets were resuspended in about 300 μl of lysis buffer: approximate concentrations (50 mM NaH₂PO₄ (pH 8.0), 300 mM NaCl, 10 mM imidazole (Sigma, #I5513), to which was added about 2 μl/ml beta-mercaptoethanol (BME)), and protease inhibitor cocktail tablet (Roche, 11836170001) (1 tablet for about 10 ml cell extract). The cell suspension was added to a 2 ml screw-cap microcentrifuge tube that had been pre-aliquotted with about 0.5 ml of acid washed glass beads (425-600 μm). Cells were lysed using a FastPrep-24 (MP Biomedicals, Solon, Ohio) at amplitude setting of about 6 for about 3 repetitions of about 1 minute. Cells were chilled on ice for about 5 minutes between repetitions. Samples were centrifuged at about 10,000×g for about 10 minutes at 4° C. Recovered supernatants were used in the XI enzyme activity assay. XI enzyme activity was performed as described in the materials and methods. Results are shown in Table 3.

TABLE 3
XI activity at pH 7.5
SEQ	Volumetric
ID NO:	Activity	FIOPC
2	−60.73	2.58
4	−21.84	0.93
6	0.86	−0.05
8	−2.14	0.12
10	−2.38	0.13
12	−12.82	0.54
14	−26.97	1.45
16	−76.50	4.12
18	−15.32	0.83
20	−5.33	0.29
22	0.48	−0.03
24	0.36	−0.02
26	0.81	−0.04
28	−6.65	0.36
30	−9.10	0.49
32	−38.10	2.05
34	−21.76	1.17
36	−13.82	0.59
38	−17.58	0.75
40	−12.34	0.52
42	−74.88	3.18
44	−37.10	1.57
46	−35.57	1.51
48	−24.69	1.05
50	−32.23	1.37
52	−26.72	1.13
54	−90.79	3.85
56	−39.89	1.69
58	−74.26	3.15
60	−11.91	0.64
62	−15.43	0.83
64	−12.98	0.70
66	−27.45	1.48
68	−29.43	1.59
70	−4.54	0.24
72	−8.93	0.48
74	−0.20	0.01
76	−0.33	0.02
78	−50.55	2.15
80	−57.13	2.42
82	−58.09	2.47
84	−46.42	1.97
86	−35.95	1.53
88	−2.16	0.09
90	−32.77	1.39
92	−30.82	1.31
94	−8.16	0.35
96	−46.18	1.96
98	−30.05	1.28
100	−8.40	0.45
102	−8.34	0.45
104	−3.80	0.20
106	−4.81	0.26
108	−12.06	0.65
110	−6.10	0.33
112	−7.71	0.42
114	−4.17	0.22
116	−7.07	0.38
118	−13.50	0.73
120	−1.15	0.06
122	0.03	0.00
124	−4.41	0.24
126	−0.85	0.05
128	−14.60	0.79
130	−17.26	0.93
132	−0.75	0.04
134	−11.55	0.62
136	−7.20	0.39
138	0.16	−0.01
140	−3.63	0.20
142	−3.63	0.20
144	−1.20	0.06
146	−16.77	0.90
148	−2.00	0.11
150	−1.40	0.08
152	−3.63	0.20
154	−7.09	0.38
156	−0.96	0.05
158	−2.79	0.15
160	−3.23	0.17
162	−10.17	0.55
164	−0.51	0.03
166	−3.43	0.19
168	−5.65	0.30
170	−2.35	0.13
172	−1.20	0.06
174	−2.29	0.12
176	−1.92	0.08
Op-XI (ABD)	−23.56	NA
Op-XI (SBD)	−18.55	NA
Vo - ctrl	−1.74	NA

5.6 Example 5: Growth of Yeast Containing XI Clones on Xylose

A subset of the XI genes from Example 3 were expressed in Saccharomyces cerevisiae CEN.PK2-1Ca (ATCC: MYA1108) and assayed for ability to confer the ability to grow on xylose. This assay was carried out as follows: colonies were isolated on SC-ura+2% glucose agar plates and inoculated into about 3 ml “pre-cultures” of both SC-ura 2% glycerol and SC-ura 2% xylose media, incubated at about 30° C., about 220 rpm, overnight. Cells were harvested by centrifugation (about 100×g, 5 minutes), supernatant discarded and washed twice and resuspended in about 1 ml of SC-ura 2% xylose. Cells were inoculated into Biolector plates, containing SC-ura, 2% xylose, and inoculums were normalized to two different starting optical densities of about OD₆₀₀ 0.2 and 0.4. Plates were covered using gas permeable seals and incubated in a BioLector microfermentation device (m2p-labs, Model G-BL100) at about 30° C. for about 4 days at 800 rpm and 90% humidity. Growth readings from the Biolector were acquired for 60-100 hours according to manufacturer's recommendations. Results are shown in FIG. 4.

5.7 Example 6: Ethanol Production Under Anaerobic Conditions

A subset of the XI expressing yeast clones in strain Saccharomyces cerevisiae CEN.PK2-1Ca (ATCC: MYA1108) were assayed for ability to ferment xylose to ethanol (EtOH). In brief, single colonies were inoculated into about 25 ml of SC-ura medium supplemented with about 0.1% glucose and about 3% xylose. Cultures were incubated under microaerobic conditions at about 30° C. and about 200 rpm. Samples were harvested at about 0, 24, 48, 72 h, and ethanol concentration determined via HPLC standard assays. Ethanol productivity was calculated, and listed in units of grams of EtOH per liter per hour, and FIOPC was generated comparing productivity of the control Op-XI. Results are shown in Table 4.

TABLE 4
Anaerobic EtOH Production
	Time (h)	EtOH
SEQ ID NO:	0	24	48	72	(g/L/h)	FIOPC
6	0.28	0	0	0	−0.004	−0.5
8	0	0	0	0	0.000	0.0
10	0	0	0	0	0.000	0.0
14	0.37	0.28	0.71	1.24	0.013	1.7
16	0.33	0.275	0.72	1.06	0.011	1.4
18	0.29	0.135	0.31	0.595	0.005	0.6
20	0.33	0	0	0	−0.004	−0.5
22	0.32	0	0	0	−0.004	−0.5
24	0.28	0	0	0	−0.004	−0.5
26	0.26	0	0	0	−0.003	−0.4
28	0.23	0.385	1.015	1.54	0.019	2.5
30	0.27	0	0	0.07	−0.003	−0.3
32	0	0.165	0.48	0.815	0.012	1.5
34	0	0.125	0.33	0.615	0.009	1.1
36	0	0	0	0	0.000	0.0
46	0	0.285	0.905	1.625	0.023	3.0
60	0.45	0.35	0.87	1.39	0.014	1.8
62	0	0	0	0.065	0.001	0.1
64	0.38	0.275	0.735	1.18	0.012	1.6
66	0	0	0.12	0.22	0.003	0.4
68	0	0.05	0.275	0.5	0.007	0.9
70	0	0	0	0	0.000	0.0
72	0.119	0	0.054	0.1685	0.001	0.1
74	0.21	0.11	0.275	0.57	0.005	0.7
76	0.28	0	0	0	−0.004	−0.5
90	0	0.24	0.69	1.09	0.016	2.0
100	0.104	0.642	0.141	0.366	0.001	0.2
102	0.185	0	0	0.054	−0.002	−0.2
104	0.235	0.536	0	0	−0.005	−0.7
106	0.188	0.4835	0	0	−0.004	−0.6
108	0.19	0.5855	0.1455	0.313	0.000	0.0
110	0.3	0	0	0.05	−0.003	−0.4
112	0.19	0.5535	0.106	0.1135	−0.003	−0.4
114	0.174	0	0	0	−0.002	−0.3
116	0.15	0	0.0515	0.211	0.001	0.1
118	0.177	0.7075	0.5065	0.941	0.009	1.1
120	0.153	0	0	0	−0.002	−0.2
122	0.169	0.553	0	0.074	−0.003	−0.5
124	0.125	0	0	0	−0.002	−0.2
126	0.32	0	0	0	−0.004	−0.5
128	0	0	0	0	0.000	0.0
130	0	0	0	0	0.000	0.0
132	0.121	0	0	0	−0.002	−0.2
134	0.118	0	0	0.1105	0.000	0.0
136	0.108	0	0	0	−0.001	−0.2
138	0.172	0.513	0	0	−0.004	−0.6
140	0.17	0.542	0	0.3135	0.000	−0.1
142	0.102	0	0	0	−0.001	−0.2
144	0.28	0	0	0	−0.004	−0.5
146	0.103	0.635	0.263	0.563	0.004	0.5
150	0.27	0	0	0	−0.003	−0.4
149	0.27	0	0	0	−0.003	−0.4
152	0.17	0	0	0	−0.002	−0.3
154	0.23	0	0	0	−0.003	−0.4
156	0.23	0	0	0	−0.003	−0.4
158	0.4	0	0.105	0.23	−0.002	−0.2
160	0.38	0	0	0	−0.005	−0.6
162	0.36	0.055	0.23	0.41	0.001	0.2
164	0.32	0	0	0	−0.004	−0.5
166	0.31	0	0	0	−0.004	−0.5
168	0.32	0	0.295	0.6	0.005	0.6
170	0.164	0.4995	0	0	−0.004	−0.5
172	0.27	0	0	0	−0.003	−0.4
174	0.3	0	0.17	0.345	0.001	0.2
OP-XI (pos)	0.2385	0.5875	0.6965	0.81508	0.008	NA
Host-(neg)	0.23625	0.088125	0	0	−0.003	NA

5.8 Example 7: Impact of pH on XI Activity

Extracts from strain Saccharomyces cerevisiae CEN.PK2-1 Ca (ATCC: MYA1108, expressing XI gene candidates in vector p426PGK1, were prepared as described in the Materials and Methods and assayed for XI activity at pH 7.5 and pH 6.0. Percent activity listed was calculated by dividing the VA at pH 6 by the VA at pH 7.5 and multiplying by 100. Results are listed in Table 5.

TABLE 5
XI activity at pH 6 and pH 7.5
		VA,	VA,	Percent
SEQ	Organism	pH 6	pH 7.5	activity
ID NO:	Classification	(U/ml)	(U/ml)	(pH 6)
2	Bacteroidales	1.92	2.59	74%
14	Bacteroides	0.32	0.98	32%
16	Bacteroides	1.16	2.40	48%
32	Bacteroides	1.17	2.21	53%
38	Firmicutes	2.46	2.77	89%
42	Firmicutes	1.71	2.18	79%
44	Firmicutes	0.19	0.25	76%
46	Firmicutes	1.49	1.95	76%
50	Firmicutes	0.81	0.95	86%
52	Firmicutes	0.02	0.08	26%
54	Neocallimastigales	1.46	2.90	51%
58	Neocallimastigales	1.89	3.05	62%
68	Neocallimastigales	1.50	1.97	76%
72	Neocallimastigales	0.57	1.04	55%
78	Prevotella	2.40	3.61	67%
80	Prevotella	1.52	2.29	66%
82	Prevotella	1.48	1.65	89%
84	Prevotella	1.79	2.96	61%
96	Prevotella	2.13	3.56	60%
116	Prevotella	0.06	0.13	47%
Host-neg		0.04	0.02	NA
Op-XI		0.61	1.25	49%

5.9 Example 8: K_m for Selected XI Clones

The K_m and V_max at pH 6 were determined for a subset of the XI clones, expressed on p426PGK1 vector in Saccharomyces cerevisiae CEN.PK2-1Ca (ATCC: MYA1108), using the XI activity assay described in the Materials and Methods and varying the concentrations of xylose from about 40-600 mM. Results shown are calculated using the Hanes Plot, which rearranges the Michaelis-Menten equation (v=V_max[S]/(K_m+[S])) as: ([S]/v=K_m/V_max+[S]/V_max), where plotting [S]/v against [S], resulting in a straight line and where the y intercept=K_m/V_max, the slope=1/V_max, and the x intercept=−K_m. Results are listed in Table 6.

TABLE 6
Km determination for 3 XIs
SEQ ID NO:	Km	Vmax
78	35.2	27.6
96	33.7	28.0
38	28.8	28.6

5.10 Example 9: Quantification of XI Activity Expressed From Single Genomic Integration Locus

A vector named pYDAB006 (FIG. 5A) for integration into locus YER131.5 (between YER131W and YER132C) in the S. cerevisiae genome was constructed using conventional cloning methods. The vector backbone with a PacI site at each end was derived from pBluescript II SK (+) (Agilent Technologies, Inc. Santa Clara, Calif.) by standard PCR techniques, which contained only the pUC origin of replication and bla gene encoding ampicillin resistance protein as a selectable marker. Two 300-base pair segments named YER131.5-A and YER 131.5-B were amplified from yeast genomic DNA by standard PCR techniques and connected with a multiple cloning site (MCS 1: 5′-GGCGCGCCTCTAGAAAGCTTACGCGTGAGCTCCCTGCAGGGATATCGGTACCGCGGCCG C-3′ (SEQ ID NO:181)) using the overlapping PCR technique. The PCR primers used in the overlapping PCR are shown in Table 7 below:

TABLE 7
Primers Used in pYDAB006 Construction
	SEQ ID
Primer	NO:	Sequence (Pad site is underlined)
131.5AF	182	caccattaattaaAGCTTTGTAAATATGATGAGAG
		AATAATATAAATCAAACG
131.5AR	183	GGCGCGCCTCTAGAAAGCTTAATCGACAAGA
		ACACTTCTATTTATATAGGTATGAAA
131.5BF	184	GCAGGGATATCGGTACCCACCAGCGGCCGCT
		GAAGAAGGTTTATTTCGTTTCGCTGT
131.5BR	185	caccattaattaaCCCAGGTGAGACTGGATGCTCCA
		TA
ABMCSF	186	GCCTCTAGAAAGCTTACGCGTGAGCTCCCTG
		CAGGGATATCGGTACCCACCAGCGGCCGC
ABMCSR	187	CGCTGGTGGGTACCGATATCCCTGCAGGGAG
		CTCACGCGTAAGCTTTCTAGAGGCGCGCC

The overlapping PCR product was then ligated with the vector backbone resulting in plasmid pYDAB006.

A vector named pYDURA01 (FIG. 5B) for generating yeast selectable and recyclable marker was constructed using similar method as pYDAB006. The URA3 expression cassette was amplified from yeast genomic DNA by standard PCR techniques. The 200 base pair fingerprint sequence (named R88: TGCGTGTGCCGCGAGTCCACGTCTACTCGCGAACCGAGTGCAGGCGGGTCTTCGGCCAG GACGGCCGTGCGTGACCCCGGCCGCCAGACGAAACGGACCGCGCTCGCCAGACGCTACC CAGCCCGTTCATGCCGGCCGCGAGCCGACCTGTCTCGGTCGCTTCGACGCACGCGCGGTC CTTTCGGGTACTCGCCTAAGAC (SEQ ID NO:188)) at both sides of URA3 cassette was amplified by standard PCR techniques from the genomic DNA of yBPA317, which was a diploid strain having genotypes MATa/MATalpha; URA3/ura3; YDL074.5::P(TDH3)-CBT1-T(CYC1)-R88 YLR388.5::P(TDH3)-StBGL-T(CYC1)-R88/YLR388.5::P(TDH3)-StBGL-T(CYC1)-R88. The primers used in the amplification are described in Table 8 below:

TABLE 8
Primers Used in pYDURA01 Construction
	SEQ	Sequence (KpnI and
Primer	ID NO:	NotI sites are underlined)
NotI-KpnI-R88-F	189	caatagcggccgcggtaccTGCGTGT
		GCCGCGAGTCCAC
R88-BamHI-R	190	TGTTAGGATCCGTCTTAGGCGAG
		TACCCGAAAGG
BamHI-ura-F	191	caataggatccAGGCATATTTATGGTG
		AAGAATAAGT
ura-Xho-R	192	TGTTACTCGAGAAATCATTACGAC
		CGAGATTCCCG
XhoI-R88-F	193	caatactcgagTGCGTGTGCCGCGAG
		TCCAC
R88-NotI-R	194	TGTTAGCGGCCGCGTCTTAGGCG
		AGTACCCGAAAGG

An expression cassette was generated for the XI genes by cloning into a vector named pYDPt005 (FIG. 5C). pYDPt005 was generated using similar method as pYDAB006. It contained a TDH3 promoter and a PGK1 terminator flanking a multiple cloning site (MCS 2: 5′-ACTAGTGGATCCCTCGAGGTCGACGTTTAAAC-3′ (SEQ ID NO:195), where single underline is SpeI site, double underline is XhoI site, and jagged underline is PmeI site). The promoter and the terminator were amplified from S. cerevisiae genomic DNA; an AscI site was added to the 5′ end of the TDH3 promoter while a KpnI site was added to the 3′ end of the PGK1 terminator during amplification. Primers used in the amplification are described in Table 9.

TABLE 9
Primers Used in pYDPt005 Construction
		Sequence (AscI and
Primer	SEQ ID NO:	KpnI sites are underlined)
TDH-F	196	CACCAGGCGCGCCTCTAGAAAGCT
		TACGCGTAGTTTATCATTATCAATA
		CTGCCATTTCAAAGA
overlap-TDH-R	197	AACGTCGACCTCGAGGGATCCAC
		TAGTTCGAAACTAAGTTCTTGGT
		GTTTTAAAACT
overlap-PGK-F	198	GTGGATCCCTCGAGGTCGA
		CGTTTAAACATTGAATTGAA
		TTGAAATCGATAGATCAAT
PGK-R	199	CACCAGCGGCCGCGGTACCGATAT
		CCCTGCAGGGAGCTCGAAATATC
		GAATGGGAAAAAAAAACTGGAT

An Orpinomyces sp. XI gene (NCBI:169733248) was cloned in this vector between the SpeI and XhoI sites. The Orpinomyces sp. XI expression cassette and R88-Ura-R88 fragment were then cloned into vector pYDAB006 using Asa KpnI and Nod sites; the resulting plasmid was named pYDABF006 (FIG. 5D). Subsequently, the Orpinomyces sp. XI gene in pYDABF0006 was replaced with a subset of the XI genes of Table 2 by digestion of pYDABF0006 with SpeI and PmeI and ligation to a DNA fragment encoding the appropriate XI sequence which had been amplified from p426PGK1-XI constructs. A SpeI site followed by a Kozak-like sequence (6 consecutive adenines) was added immediately in front of the start codon of the XI genes while a PmeI site was added to the 3′ end of the XI genes during amplification.

XI gene integration cassettes were extracted by PacI digestion and used to transform yeast strain yBPA130 using standard techniques. Transformants were selected for growth on SC-Ura (Synthetic Complete, Ura dropout) agar plates. Integration position and existence of XI cassette in transformants was confirmed by PCR using the primers shown in Table 10.

TABLE 10
Primers Used in Integration Verification
	SEQ
Primer	ID NO:	Sequence
5′ of integration	200	ACAGGGATAACAAAGTTTCTCCAGC
3′ of integration	201	CATACCAAGTCATGCGTTACCAGAG
5′ of R88-ura-R88	202	TTTCCCATTCGATATTTCGAGCTCC
3′ of integration	203	CATACCAAGTCATGCGTTACCAGAG

Confirmed clones were then grown about 18 hours in liquid YPD to allow looping out of the URA3 marker and were selected for growth on SC+5-FOA agar plate. The absence of the URA3 marker was confirmed by PCR.

Strains containing the confirmed XI expression cassettes were inoculated into about 3 ml of modified YP Media (YP+0.1% Glucose+3.0% Xylose) and incubated overnight at about 30° C. and about 220 rpm. These overnight cultures were subcultured into about 25 ml of the same media to about OD₆₀₀=0.2. Samples were incubated overnight at about 30° C. and about 220 rpm. Cultures were harvested when OD₆₀₀ was between about 3 and 4. Pellets were collected by centrifugation for about 5 minutes at about 4000 rpm. The supernatant was discarded and pellets washed with about 25 ml of distilled-deionized water and centrifuged again using the same conditions. Supernatant was discarded and the pellet frozen at about −20° C. until lysis and characterization.

Cell pellets were thawed and about 200 mg of each pellet sample was weighed out into 2 ml microcentrifuge tubes. About 50 μl of Complete®, EDTA-free Protease Inhibitor cocktail (Roche Part#11873 580 001) at 5 times the concentration stated in the manufacturer's protocol was added to each sample. To this was added about 0.5 ml of Y-PER Plus® Dialyzable Yeast Protein Extraction Reagent (Thermo Scientific Part#78999) (YP+) to each sample. Samples were incubated at about 25° C. for about 4 hours on rotating mixer. Sample supernatants were collected after centrifugation at about 10,000×g for about 10 minutes for characterization.

Total protein concentrations of the XI sample extracts prepared above were carried out using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, cat#500-0006, Hercules Calif.) which is a modified version of the Bradford method (Bradford).

Yeast physiological pH ranges are known to range from about pH 6 to about pH 7.5 (Pena, Ramirez et al., 1995, J. Bacteriology 4:1017-1022). Ranking of XI activity at yeast physiological pH was accomplished using the assay conditions at pH 7.5 and modified for pH 6.0 as described in the materials and methods. The specific activities of 20 XIs when expressed from a single copy integrated into the yeast YER131.5 locus were evaluated. The results are listed in Table 11.

TABLE 11
SA of XI Expressed in an Industrial S. cerevisiae
SEQ	Organism	SA, pH 6	SA, pH 7.5
ID NO:	Classification	(U/mg)	(U/mg)
2	Bacteroidales	0.86	1.08
14	Bacteroides	0.33	1.07
16	Bacteroides	0.57	1.05
32	Bacteroides	0.53	1.00
38	Firmicutes	1.00	0.94
42	Firmicutes	0.79	0.82
44	Firmicutes	0.08	0.10
46	Firmicutes	0.62	0.69
50	Firmicutes	0.35	0.41
52	Firmicutes	0.01	0.03
54	Neocallimastigales	0.64	1.17
58	Neocallimastigales	0.79	1.10
68	Neocallimastigales	0.01	0.02
72	Neocallimastigales	0.22	0.40
78	Prevotella	1.10	1.45
80	Prevotella	0.74	1.11
82	Prevotella	0.54	0.60
84	Prevotella	0.76	1.06
96	Prevotella	1.10	1.62
116	Prevotella	0.03	0.06
Host neg Ctrl		0.00	0.02

5.11 Example 10: Identification of Sequence Motifs in Acid Tolerant XIs

The proposed mechanism of xylose isomerases can be summarized as follows: (i) binding of xylose to xylose isomerase, so that O3 and O4 are coordinated by metal ion I; (ii) enzyme-catalyzed ring opening (the identity of the ring-opening group remains a subject for further investigation; ring opening may be the rate limiting step in the overall isomerization process); (iii) chain extension (sugar binds in a linear extended form) in which O2 and O4 now coordinate metal ion I; (iv) O2 becomes deprotonated causing a shift of metal ion II from position 1 to an adjacent position 2 in which it coordinates O1 and O2 of the sugar together with metal ion I; (v) isomerization via an anionic transition state arises by a hydride shift promoted by electrophilic catalysis provided by both metal ions; (vi) collapse of transition state by return of metal ion II to position 1; (vii) chain contraction to a pseudo-cyclic position with ligands to metal ion I changing from O2/O4 back to O3/O4; (viii) enzyme-catalyzed ring closure; (ix) dissociation of xylulose from xylose isomerase (Lavie et al., 1994, Biochemistry 33(18), 5469-5480).

Many XIs identified contained one or both of two signature sequences characteristic of XIs, [LI]EPKP.{2}P (SEQ ID NO:204) and [FL]HD[^K]D[LIV].[PD].[GDE] (SEQ ID NO:205). Additional sequence motifs present in the top performing Firmicutes and Prevotella XIs were identified. The motifs are located near the active site including residues in direct contact with the D-xylose and/or the metal ions. The motifs are shown in Table 12 below:

TABLE 12
XI Sequence Motifs
XI Source	Motif	Sequence	SEQ ID NO:
Firmicutes	1A	P[FY][AST][MLVI][AS][WYFL]W[HT]N[LFMG]GA	206
Firmicutes	1B	P[FY][AS].{2}[WYFL]W[HT][{circumflex over ( )}TV].GA	207
Firmicutes	2	[GSN][IVA]R[YFHG][FYLIV]C[FW]HD.D	208
Firmicutes	3	T[ASTC][NK][{circumflex over ( )}L]F. [NDH][PRKAG][RVA][FY]C	209
Firmicutes	4	[WFY]D[TQVI]D.[FY][PF][{circumflex over ( )}T].{2,4}[YFH]S[ATL]T	210
Firmicutes	5	GF[NH]FD[SA]KTR	211
Prevotella	1A	FG.QT[RK].{2}E[WYF][DNG].{2,3}[DNEGT][AT]	212
Prevotella	1B	FG.QT[RK].{2}E[WYF][DNG].{3}[{circumflex over ( )}C][AP]	213
Prevotella	2	[FW]HD.D[LVI].[DE]EG[{circumflex over ( )}P][TSD][IV][EA]E	214

5.12 Example 11: In Vivo Evaluation of Xylose Isomerase

Haploid S. cerevisiae strain yBPA130 (MATa::ura3) and yBPA136 (MATalpha::ura3) were genetically modified to enhance C5 xylose utilization during fermentation. The modification includes the following: the native glucose repressible alcohol dehydrogenase II gene ADH2 was disrupted by inserting an expression cassette of the endogenous transaldolase gene TAL1 (SEQ ID N0:215) and xylulokinase gene XKS1 (SEQ ID NO:216). PHO13 encoding the native alkaline phosphatase specific for p-nitrophenyl phosphate gene was disrupted by inserting the native transketolase-1 gene TKL1 (SEQ ID NO:217). Native aldose reductase gene GRE3 was disrupted by inserting native D-ribulose-5-phosphate 3-epimerase gene RPE1 (SEQ ID NO:218) and Ribose-5-phosphate ketol-isomerase gene RKI1 (SEQ ID NO:219). Also one expression cassette of native galactose permease gene GAL2 (SEQ ID NO: 220) was integrated into the S. cerevisiae strain, resulting in haploid strains pBPB007 (MATa::ura3) and pBPB008 (MATalpha::ura3). The genotype of pBPB007 and pBPB008 is adh2::TAL1-XKS1, pho13::TKL1-XKS1, gre3::RPE1-RKI1 and YLR388.5::GAL2. The sequences are shown in Table 13, below:

TABLE 13
Sequence	Type of	SEQ ID
Name	sequence	NO:	Sequence
TAL1 (S. cerevisiae)	DNA	215	ATGTCTGAACCAGCTCAAAAGAAACAAAAGGTTGCTAACAACTCT
			CTAGAACAATTGAAAGCCTCCGGCACTGTCGTTGTTGCCGACACT
			GGTGATTTCGGCTCTATTGCCAAGTTTCAACCTCAAGACTCCACA
			ACTAACCCATCATTGATCTTGGCTGCTGCCAAGCAACCAACTTAC
			GCCAAGTTGATCGATGTTGCCGTGGAATACGGTAAGAAGCATGGT
			AAGACCACCGAAGAACAAGTCGAAAATGCTGTGGACAGATTGTTA
			GTCGAATTCGGTAAGGAGATCTTAAAGATTGTTCCAGGCAGAGTC
			TCCACCGAAGTTGATGCTAGATTGTCTTTTGACACTCAAGCTACC
			ATTGAAAAGGCTAGACATATCATTAAATTGTTTGAACAAGAAGGT
			GTCTCCAAGGAAAGAGTCCTTATTAAAATTGCTTCCACTTGGGAA
			GGTATTCAAGCTGCCAAAGAATTGGAAGAAAAGGACGGTATCCAC
			TGTAATTTGACTCTATTATTCTCCTTCGTTCAAGCAGTTGCCTGT
			GCCGAGGCCCAAGTTACTTTGATTTCCCCATTTGTTGGTAGAATT
			CTAGACTGGTACAAATCCAGCACTGGTAAAGATTACAAGGGTGAA
			GCCGACCCAGGTGTTATTTCCGTCAAGAAAATCTACAACTACTAC
			AAGAAGTACGGTTACAAGACTATTGTTATGGGTGCTTCTTTCAGA
			AGCACTGACGAAATCAAAAACTTGGCTGGTGTTGACTATCTAACA
			ATTTCTCCAGCTTTATTGGACAAGTTGATGAACAGTACTGAACCT
			TTCCCAAGAGTTTTGGACCCTGTCTCCGCTAAGAAGGAAGCCGGC
			GACAAGATTTCTTACATCAGCGACGAATCTAAATTCAGATTCGAC
			TTGAATGAAGACGCTATGGCCACTGAAAAATTGTCCGAAGGTATC
			AGAAAATTCTCTGCCGATATTGTTACTCTATTCGACTTGATTGAA
			AAGAAAGTTACCGCTTAA
XKS1 (S. cerevisiae)	DNA	216	ATGTTGTGTTCAGTAATTCAGAGACAGACAAGAGAGGTTTCCAAC
			ACAATGTCTTTAGACTCATACTATCTTGGGTTTGATCTTTCGACC
			CAACAACTGAAATGTCTCGCCATTAACCAGGACCTAAAAATTGTC
			CATTCAGAAACAGTGGAATTTGAAAAGGATCTTCCGCATTATCAC
			ACAAAGAAGGGTGTCTATATACACGGCGACACTATCGAATGTCCC
			GTAGCCATGTGGTTAGAGGCTCTAGATCTGGTTCTCTCGAAATAT
			CGCGAGGCTAAATTTCCATTGAACAAAGTTATGGCCGTCTCAGGG
			TCCTGCCAGCAGCACGGGTCTGTCTACTGGTCCTCCCAAGCCGAA
			TCTCTGTTAGAGCAATTGAATAAGAAACCGGAAAAAGATTTATTG
			CACTACGTGAGCTCTGTAGCATTTGCAAGGCAAACCGCCCCCAAT
			TGGCAAGACCACAGTACTGCAAAGCAATGTCAAGAGTTTGAAGAG
			TGCATAGGTGGGCCTGAAAAAATGGCTCAATTAACAGGGTCCAGA
			GCCCATTTTAGATTTACTGGTCCTCAAATTCTGAAAATTGCACAA
			TTAGAACCAGAAGCTTACGAAAAAACAAAGACCATTTCTTTAGTG
			TCTAATTTTTTGACTTCTATCTTAGTGGGCCATCTTGTTGAATTA
			GAGGAGGCAGATGCCTGTGGTATGAACCTTTATGATATACGTGAA
			AGAAAATTCAGTGATGAGCTACTACATCTAATTGATAGTTCTTCT
			AAGGATAAAACTATCAGACAAAAATTAATGAGAGCACCCATGAAA
			AATTTGATAGCGGGTACCATCTGTAAATATTTTATTGAGAAGTAC
			GGTTTCAATACAAACTGCAAGGTCTCTCCCATGACTGGGGATAAT
			TTAGCCACTATATGTTCTTTACCCCTGCGGAAGAATGACGTTCTC
			GTTTCCCTAGGAACAAGTACTACAGTTCTTCTGGTCACCGATAAG
			TATCACCCCTCTCCGAACTATCATCTTTTC
			ATTCATCCAACTCTGCCAAACCATTATATGGGTATGATTTGTTAT
			TGTAATGGTTCTTTGGCAAGGGAGAGGATAAGAGACGAGTTAAAC
			AAAGAACGGGAAAATAATTATGAGAAGACTAACGATTGGACTCTT
			TTTAATCAAGCTGTGCTAGATGACTCAGAAAGTAGTGAAAATGAA
			TTAGGTGTATATTTTCCTCTGGGGGAGATCGTTCCTAGCGTAAAA
			GCCATAAACAAAAGGGTTATCTTCAATCCAAAAACGGGTATGATT
			GAAAGAGAGGTGGCCAAGTTCAAAGACAAGAGGCACGATGCCAAA
			AATATTGTAGAATCACAGGCTTTAAGTTGCAGGGTAAGAATATCT
			CCCCTGCTTTCGGATTCAAACGCAAGCTCACAACAGAGACTGAAC
			GAAGATACAATCGTGAAGTTTGATTACGATGAATCTCCGCTGCGG
			GACTACCTAAATAAAAGGCCAGAAAGGACTTTTTTTGTAGGTGGG
			GCTTCTAAAAACGATGCTATTGTGAAGAAGTTTGCTCAAGTCATT
			GGTGCTACAAAGGGTAATTTTAGGCTAGAAACACCAAACTCATGT
			GCCCTTGGTGGTTGTTATAAGGCCATGTGGTCATTGTTATATGAC
			TCTAATAAAATTGCAGTTCCTTTTGATAAATTTCTGAATGACAAT
			TTTCCATGGCATGTAATGGAAAGCATATCCGATGTGGATAATGAA
			AATTGGGATCGCTATAATTCCAAGATTGTCCCCTTAAGCGAACTG
			GAAAAGACTCTCATCTAA
TKL1 (S. cerevisiae)	DNA	217	ATGACTCAATTCACTGACATTGATAAGCTAGCCGTCTCCACCATA
			AGAATTTTGGCTGTGGACACCGTATCCAAGGCCAACTCAGGTCAC
			CCAGGTGCTCCATTGGGTATGGCACCAGCTGCACACGTTCTATGG
			AGTCAAATGCGCATGAACCCAACCAACCCAGACTGGATCAACAGA
			GATAGATTTGTCTTGTCTAACGGTCACGCGGTCGCTTTGTTGTAT
			TCTATGCTACATTTGACTGGTTACGATCTGTCTATTGAAGACTTG
			AAACAGTTCAGACAGTTGGGTTCCAGAACACCAGGTCATCCTGAA
			TTTGAGTTGCCAGGTGTTGAAGTTACTACCGGTCCATTAGGTCAA
			GGTATCTCCAACGCTGTTGGTATGGCCATGGCTCAAGCTAACCTG
			GCTGCCACTTACAACAAGCCGGGCTTTACCTTGTCTGACAACTAC
			ACCTATGTTTTCTTGGGTGACGGTTGTTTGCAAGAAGGTATTTCT
			TCAGAAGCTTCCTCCTTGGCTGGTCATTTGAAATTGGGTAACTTG
			ATTGCCATCTACGATGACAACAAGATCACTATCGATGGTGCTACC
			AGTATCTCATTCGATGAAGATGTTGCTAAGAGATACGAAGCCTAC
			GGTTGGGAAGTTTTGTACGTAGAAAATGGTAACGAAGATCTAGCC
			GGTATTGCCAAGGCTATTGCTCAAGCTAAGTTATCCAAGGACAAA
			CCAACTTTGATCAAAATGACCACAACCATTGGTTACGGTTCCTTG
			CATGCCGGCTCTCACTCTGTGCACGGTGCCCCATTGAAAGCAGAT
			GATGTTAAACAACTAAAGAGCAAATTCGGTTTCAACCCAGACAAG
			TCCTTTGTTGTTCCACAAGAAGTTTACGACCACTACCAAAAGACA
			ATTTTAAAGCCAGGTGTCGAAGCCAACAACAAGTGGAACAAGTTG
			TTCAGCGAATACCAAAAGAAATTCCCAGAATTAGGTGCTGAATTG
			GCTAGAAGATTGAGCGGCCAACTACCCGCA
			AATTGGGAATCTAAGTTGCCAACTTACACCGCCAAGGACTCTGCC
			GTGGCCACTAGAAAATTATCAGAAACTGTTCTTGAGGATGTTTAC
			AATCAATTGCCAGAGTTGATTGGTGGTTCTGCCGATTTAACACCT
			TCTAACTTGACCAGATGGAAGGAAGCCCTTGACTTCCAACCTCCT
			TCTTCCGGTTCAGGTAACTACTCTGGTAGATACATTAGGTACGGT
			ATTAGAGAACACGCTATGGGTGCCATAATGAACGGTATTTCAGCT
			TTCGGTGCCAACTACAAACCATACGGTGGTACTTTCTTGAACTTC
			GTTTCTTATGCTGCTGGTGCCGTTAGATTGTCCGCTTTGTCTGGC
			CACCCAGTTATTTGGGTTGCTACACATGACTCTATCGGTGTCGGT
			GAAGATGGTCCAACACATCAACCTATTGAAACTTTAGCACACTTC
			AGATCCCTACCAAACATTCAAGTTTGGAGACCAGCTGATGGTAAC
			GAAGTTTCTGCCGCCTACAAGAACTCTTTAGAATCCAAGCATACT
			CCAAGTATCATTGCTTTGTCCAGACAAAACTTGCCACAATTGGAA
			GGTAGCTCTATTGAAAGCGCTTCTAAGGGTGGTTACGTACTACAA
			GATGTTGCTAACCCAGATATTATTTTAGTGGCTACTGGTTCCGAA
			GTGTCTTTGAGTGTTGAAGCTGCTAAGACTTTGGCCGCAAAGAAC
			ATCAAGGCTCGTGTTGTTTCTCTACCAGATTTCTTCACTTTTGAC
			AAACAACCCCTAGAATACAGACTATCAGTCTTACCAGACAACGTT
			CCAATCATGTCTGTTGAAGTTTTGGCTACCACATGTTGGGGCAAA
			TACGCTCATCAATCCTTCGGTATTGACAGATTTGGTGCCTCCGGT
			AAGGCACCAGAAGTCTTCAAGTTCTTCGGTTTCACCCCAGAAGGT
			GTTGCTGAAAGAGCTCAAAAGACCATTGCATTCTATAAGGGTGAC
			AAGCTAATTTCTCCTTTGAAAAAAGCTTTCTAA
RPE1 (S. cerevisiae)	DNA	218	ATGGTCAAACCAATTATAGCTCCCAGTATCCTTGCTTCTGACTTC
			GCCAACTTGGGTTGCGAATGTCATAAGGTCATCAACGCCGGCGCA
			GATTGGTTACATATCGATGTCATGGACGGCCATTTTGTTCCAAAC
			ATTACTCTGGGCCAACCAATTGTTACCTCCCTACGTCGTTCTGTG
			CCACGCCCTGGCGATGCTAGCAACACAGAAAAGAAGCCCACTGCG
			TTCTTCGATTGTCACATGATGGTTGAAAATCCTGAAAAATGGGTC
			GACGATTTTGCTAAATGTGGTGCTGACCAATTTACGTTCCACTAC
			GAGGCCACACAAGACCCTTTGCATTTAGTTAAGTTGATTAAGTCT
			AAGGGCATCAAAGCTGCATGCGCCATCAAACCTGGTACTTCTGTT
			GACGTTTTATTTGAACTAGCTCCTCATTTGGATATGGCTCTTGTT
			ATGACTGTGGAACCTGGGTTTGGAGGCCAAAAATTCATGGAAGAC
			ATGATGCCAAAAGTGGAAACTTTGAGAGCCAAGTTCCCCCATTTG
			AATATCCAAGTCGATGGTGGTTTGGGCAAGGAGACCATCCCGAAA
			GCCGCCAAAGCCGGTGCCAACGTTATTGTCGCTGGTACCAGTGTT
			TTCACTGCAGCTGACCCGCACGATGTTATCTCCTTCATGAAAGAA
			GAAGTCTCGAAGGAATTGCGTTCTAGAGATTTGCTAGATTAG
RKI1 (S. cerevisiae)	DNA	219	ATGGCTGCCGGTGTCCCAAAAATTGATGCGTTAGAATCTTTGGGC
			AATCCTTTGGAGGATGCCAAGAGAGCTGCAGCATACAGAGCAGTT
			GATGAAAATTTAAAATTTGATGATCACAAAATTATTGGAATTGGT
			AGTGGTAGCACAGTGGTTTATGTTGCCGAAAGAATTGGACAATAT
			TTGCATGACCCTAAATTTTATGAAGTAGCGTCTAAATTCATTTGC
			ATTCCAACAGGATTCCAATCAAGAAACTTGATTTTGGATAACAAG
			TTGCAATTAGGCTCCATTGAACAGTATCCTCGCATTGATATAGCG
			TTTGACGGTGCTGATGAAGTGGATGAGAATTTACAATTAATTAAA
			GGTGGTGGTGCTTGTCTATTTCAAGAAAAATTGGTTAGTACTAGT
			GCTAAAACCTTCATTGTCGTTGCTGATTCAAGAAAAAAGTCACCA
			AAACATTTAGGTAAGAACTGGAGGCAAGGTGTTCCCATTGAAATT
			GTACCTTCCTCATACGTGAGGGTCAAGAATGATCTATTAGAACAA
			TTGCATGCTGAAAAAGTTGACATCAGACAAGGAGGTTCTGCTAAA
			GCAGGTCCTGTTGTAACTGACAATAATAACTTCATTATCGATGCG
			GATTTCGGTGAAATTTCCGATCCAAGAAAATTGCATAGAGAAATC
			AAACTGTTAGTGGGCGTGGTGGAAACAGGTTTATTCATCGACAAC
			GCTTCAAAAGCCTACTTCGGTAATTCTGACGGTAGTGTTGAAGTT
			ACCGAAAAGTGA
GAL2 (S. cerevisiae)	DNA	220	ATGGCAGTTGAGGAGAACAATATGCCTGTTGTTTCACAGCAACCC
			CAAGCTGGTGAAGACGTGATCTCTTCACTCAGTAAAGATTCCCAT
			TTAAGCGCACAATCTCAAAAGTATTCTAATGATGAATTGAAAGCC
			GGTGAGTCAGGGTCTGAAGGCTCCCAAAGTGTTCCTATAGAGATA
			CCCAAGAAGCCCATGTCTGAATATGTTACCGTTTCCTTGCTTTGT
			TTGTGTGTTGCCTTCGGCGGCTTCATGTTTGGCTGGGATACCGGT
			ACTATTTCTGGGTTTGTTGTCCAAACAGACTTTTTGAGAAGGTTT
			GGTATGAAACATAAGGATGGTACCCACTATTTGTCAAACGTCAGA
			ACAGGTTTAATCGTCGCCATTTTCAATATTGGCTGTGCCTTTGGT
			GGTATTATACTTTCCAAAGGTGGAGATATGTATGGCCGTAAAAAG
			GGTCTTTCGATTGTCGTCTCGGTTTATATAGTTGGTATTATCATT
			CAAATTGCCTCTATCAACAAGTGGTACCAATATTTCATTGGTAGA
			ATCATATCTGGTTTGGGTGTCGGCGGCATCGCCGTCTTATGTCCT
			ATGTTGATCTCTGAAATTGCTCCAAAGCACTTGAGAGGCACACTA
			GTTTCTTGTTATCAGCTGATGATTACTGCAGGTATCTTTTTGGGC
			TACTGTACTAATTACGGTACAAAGAGCTATTCGAACTCAGTTCAA
			TGGAGAGTTCCATTAGGGCTATGTTTCGCTTGGTCATTATTTATG
			ATTGGCGCTTTGACGTTAGTTCCTGAATCCCCACGTTATTTATGT
			GAGGTGAATAAGGTAGAAGACGCCAAGCGTTCCATTGCTAAGTCT
			AACAAGGTGTCACCAGAGGATCCTGCCGTCCAGGCAGAGTTAGAT
			CTGATCATGGCCGGTATAGAAGCTGAAAAACTGGCTGGCAATGCG
			TCCTGGGGGGAATTATTTTCCACCAAGACCAAAGTATTTCAACGT
			TTGTTGATGGGTGTGTTTGTTCAAATGTTC
			CAACAATTAACCGGTAACAATTATTTTTTCTACTACGGTACCGTT
			ATTTTCAAGTCAGTTGGCCTGGATGATTCCTTTGAAACATCCATT
			GTCATTGGTGTAGTCAACTTTGCCTCCACTTTCTTTAGTTTGTGG
			ACTGTCGAAAACTTGGGACATCGTAAATGTTTACTTTTGGGCGCT
			GCCACTATGATGGCTTGTATGGTCATCTACGCCTCTGTTGGTGTT
			ACTAGATTATATCCTCACGGTAAAAGCCAGCCATCTTCTAAAGGT
			GCCGGTAACTGTATGATTGTCTTTACCTGTTTTTATATTTTCTGT
			TATGCCACAACCTGGGCGCCAGTTGCCTGGGTCATCACAGCAGAA
			TCATTCCCACTGAGAGTCAAGTCGAAATGTATGGCGTTGGCCTCT
			GCTTCCAATTGGGTATGGGGGTTCTTGATTGCATTTTTCACCCCA
			TTCATCACATCTGCCATTAACTTCTACTACGGTTATGTCTTCATG
			GGCTGTTTGGTTGCCATGTTTTTTTATGTCTTTTTCTTTGTTCCA
			GAAACTAAAGGCCTATCGTTAGAAGAAATTCAAGAATTATGGGAA
			GAAGGTGTTTTACCTTGGAAATCTGAAGGCTGGATTCCTTCATCC
			AGAAGAGGTAATAATTACGATTTAGAGGATTTACAACATGACGAC
			AAACCGTGGTACAAGGCCATGCTAGAATAA

A vector named pYDAB008 rDNA (FIG. 6) for integration xylose isomerase into ribosomal DNA loci in S. cerevisiae genome was constructed using conventional cloning methods. This vector can confer high copy number integration of genes and resulting in high-level expression of proteins. The vector was derived from pBluescript II SK (+) (Agilent Technologies, Inc., Santa Clara, Calif.). The pUC origin of replication and bla gene encoding ampicillin resistance was amplified with specific primer sequences as a selectable marker for cloning. A 741 base-pair segment R1 region, 253 base-pair R3 region and a 874 base-pair R2 region were amplified from yeast genomic DNA by PCR amplifications. A multiple cloning site of SEQ ID NO:181 (: 5′-GGCGCGCCTCTAGAAAGCTTACGCGTGAGCTCCCTGCAGGGATATCGGTACCGCGGCCG C-3′) was inserted between the R1 and R3/R2 regions by assembly using overlapping PCR. All primers used in above reactions are shown in Table 14. Overlapping PCR products were then ligated in one reaction and result in rDNA integration plasmid named pYDAB008 rDNA (FIG. 6).

TABLE 14
Primers Used in pYDAB008 rDNA vector construction
		Sequence
		(Pac I restriction
Primer	SEQ ID NO:	site is underlined)
Pac I-rDNA(R1)-R	221	CACCATTAATTAACCCGGGGCA
		CCTGTCACTTTGGAA
rDNA (R1)-over-R	222	CGCGTAAGCTTTCTAGAGGCGC
		GCCAAGCTTTTACACTCTTGAC
		CAGCGCA
AB vector-MCS-R	223	CCGCTGGTGGGTACCGATATCC
		CTGCAGGGAGCTCACGCGTAAG
		CTTTCTAGAGGCG
rDNA(R3)-over-R	224	CTGCAGGGATATCGGTACCCAC
		CAGCGGCCGCAGGCCTTGGGTG
		CTTGCTGGCGAA
rDNA(R3)-over-R	225	ACCTCTGCATGCGAATTCTTAA
		GACAAATAAAATTTATAGAGAC
		TTGT
rDNA(R2)-over-R	226	GTCTTAAGAATTCGCATGCAGA
		GGTAGTTTCAAGGT
Pac I-rDNA(R2)-R	227	CACCATTAATTAATACGTATT
		TCTCGCCGAGAAAAACTT

pYDABF 0015 (comprising a nucleic acid encoding a xylose isomerase of SEQ ID NO:78) and pYDABF-0026 (comprising a nucleic acid encoding a xylose isomerase of SEQ ID NO:96) (both described in Example 10) were digested with Asc I and Kpn I restriction enzymes (New England Biolabs Inc., MA, USA) and ligated to pYDAB008 rDNA integration vector described above (FIG. 6). The resulting plasmids were named pYDABF-0033 (SEQ ID NO:78) and pYDABF-0036 (SEQ ID NO:96).

The rDNA integration cassette was linearized by Pac I restriction enzyme digestion (New England Biolabs Inc., MA, USA) and purified with DNA column purification kit (Zymo Research, Irvine, Calif., USA). The integration cassette was transformed into modified haploid S. cerevisiae strain pBPB007 (MATa::ura3) and pBPB008 (MAT alpha::ura3) using the standard protocol described in previous examples. Transformants were plated on SC-xylose (SC complete+2% xylose) agar plates, about 2-3 days at about 30° C. Colonies that grew on SC-xylose agar plates were then checked by colony PCR analysis with primer sets shown in Table 15 (SEQ ID NOs:228, 229, 230 and 231) to confirm the presence of xylose isomerase in the genome.

TABLE 15
Primers Used in Integration Verification
		SEQ
	Primer	ID NO:	Sequence
	N16PCR_F	228	CCCCATCGACAACTACGAGCTCACT
	N16PCR_R	229	CAACTTGCCGTCCTCGAAGTCCTTG
	N05PCR_F	230	CGAGCCTGAGAAGGTCGTGATGGGA
	N05PCR_R	231	TACGTCGAAGTCGGGGTTGGTAGAA

Confirmed haploid strains were BD31328 (MATa), BD31336 (MATalpha), BD31526 (MATa) and BD31527 (MATalpha). Diploid strains BD31378 (expressing a xylose isomerase of SEQ ID NO:96) and BD31365 (expressing a xylose isomerase of SEQ ID NO:78) were generated by conventional plate mating on YPXylose (YP+2% xylose) agar plates, about 2 days at about 30° C. Colony PCR with specific primers checking mating types were performed (shown in Table 14) and a single colony, which has both MATa and MATalpha were picked as diploid strains BD 31378 (SEQ ID NO: 96) and BD31365 (SEQ ID NO:78).

A linear fragment encoding the URA3 sequence (SEQ ID NO:237; TTAATTAAGTTAATTACCTTTTTTGCGAGGCATATTTATGGTGAAGAATAAGTTTTGACC ATCAAAGAAGGTTAATGTGGCTGTGGTTTCAGGGTCCATAAAGCTTTTCAATTCATCATT TTTTTTTTATTCTTTTTTTTGATTCCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGA ACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGT AGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAAC CTGCAGGAAACGAAGATAAATCATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTC ATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGT GTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTC CCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCA CAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAA AATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAG CAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGT TTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGA ATTGTCATGCAAGGGCTCCCTAGCTACTGGAGAATATACTAAGGGTACTGTTGACATTGC GAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAG ATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGAC GCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATT ATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTA CAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAAAAAACT GTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATAT CAGTTATTACCCGGGAATCTCGGTCGTAATGATTTTTATAATGACGAAAAAAAAAAAATT GGAAAGAAAAAGCTTCATGGCCTTTATAAAAAGGAACCATCCAATACCTCGCCAGAACC AAGTAACAGTATTTTACGGTTAATTAA) was transformed into BD 31378 (SEQ ID NO:96) and BD31365 (SEQ ID NO: 78) by a conventional transformation protocol, and transformants were plated on SCXylose-URA (Synthetic Complete, Uracil dropout) for selection. Colonies were checked by PCR with primers shown in Table 16, SEQ ID NO: 235, SEQ ID NO:236). Confirmed strains are BD31446 (SEQ ID NO: 78) and BD31448 (SEQ ID NO: 96).

TABLE 16
Primers Used in Mating Type Verification
	SEQ
Primer	ID NO:	Sequence
1-mating type-R	232	AGTCACATCAAGATCGTTTAT
2-mating type alpha-F	233	GCACGGAATATGGGACTACTT
3-mating type a-F	234	ACTCCACTTCAAGTAAGAGTT
Ura fix-F	235	GAACAAAAACCTGCAGGAAACG
		AAGAT
Ura fix-R	236	GCTCTAATTTGTGAGTTTAGTA
		TACATGCAT

Table 17 below shows the genotypes of the resulting yeast strains:

TABLE 17
Strain Construction
Name	Parent Strain	Description
pBPB007	yBPA130	MATa, ura3, adh2 :: TAL1-XKS1, pho13::
		TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2
pBPB008	yBPA136	MATalpha, ura3, adh2 :: TAL1-XKS1,
		pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2
BD31328	pBPB007	MATa, ura3, adh2 :: TAL1-XKS1, pho13::
		TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2, rDNA::XI (SEQ ID NO:
		96)
BD31336	pBPB008	MATalpha, ura3, adh2 :: TAL1-XKS1,
		pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2, rDNA::XI (SEQ ID NO:
		96)
BD31526	pBPB007	MATa, ura3, adh2 :: TAL1-XKS1, pho13::
		TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2, rDNA::XI (SEQ ID NO:
		78)
BD31527	pBPB008	MATalpha, ura3, adh2 :: TAL1-XKS1,
		pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2, rDNA::XI (SEQ ID NO:
		78)
BD31378	BD31328	MATa/alpha, ura3, adh2 :: TAL1-XKS1,
	BD31336	pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2, rDNA::XI (SEQ ID NO:
		96)
BD31365	BD31526	MATa/alpha, ura3, adh2 :: TAL1-XKS1,
	BD31527	pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2, rDNA::XI (SEQ ID NO:
		78)
BD31448	BD31378	MATa/alpha, adh2 :: TAL1-XKS1, pho13::
		TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2, rDNA::XI (SEQ ID NO:
		96)
BD31446	BD31365	MATa/alpha. adh2 :: TAL1-XKS1, pho13::
		TKL1-XKS1, gre3:: RPE1-RKI1 and
		YLR388.5:: GAL2, rDNA::XI (SEQ ID NO:
		78)

5.13 Example 12: Fermentation Performance of Yeast Strain Expressing Different Xylose Isomerases

Fermentation performances of two different XI-expressing yeast strains were evaluated using the DasGip fermentation systems (Eppendorf, Inc.). DasGip fermenters allowed close control over agitation, pH, and temperature ensuring consistency of the environment during fermentation. DasGip fermenters were used to test performance of the yeast strains expressing the XI genes on hydrolysate (Hz) (neutralized with magnesium bases) as a primary carbon source. Prior to the start of fermentation strains were subjected to propagation testing consisting of two steps as described below.

Seed 1:

About 1 ml of strain glycerol stock was inoculated into about 100 ml of YP (Yeast extract, Peptone) medium containing about 2% glucose and about 1% xylose in the 250 ml bellco baffled flask (Bellco, Inc.). Strains were cultivated at about 30° C. with about 200 rpm agitation for at least 18 hours until at full saturation. Optical density was assessed by measuring light absorbance at wavelength of 600 nm.

Seed 2:

About 20 ml of saturated SEED 1 (see preceding paragraph) was inoculated into 3 L Bioflo unit (New Brunswick, Inc.) containing about 2.1 L of basal medium at pH 6.0 (1% v/v inoculation). Cultivation was conducted at about 30° C. in a fed batch mode with constant air flow of about 2 L/min. Agitation ramp (rpm) was about 200-626 rpm over about 15 hours starting at about 5 hours of elapsed fermentation time (EFT). Feeding profile was about 0-4.8 ml/min over 20 hours. The basal medium contained (per 1 L): about 20% of neutralized hydrolysate (Hz); about 20 g/L sucrose (from cane juice); about 35 ml of nutrients mixture (Table 18), about 1 ml of vitamin mixture (Table 19); about 0.4 ml of antifoam 1410 (Dow Corning, Inc.) and water. Feed medium contained (per 1 L): about 20% neutralized hydrolysate (Hz), about 110 g/L sucrose (from cane juice), about 35 ml of nutrient mixture; about 1 ml of vitamin mixture, about 0.4 ml of antifoam 1410 (Dow Corning, Inc.) and water.

TABLE 18
Nutrients mixture
	Component	FW g/mol	Conc.
	KH2PO4 H2O	154.1	99.1 g/L
	Urea	60.06	65.6 g/L
	MgSO4—7H2O	192.4	14.6 g/L
	DI Water	NA	To 1.0 L

TABLE 19
Vitamin mixture (1000x)
Components mM
ZnSO4      100
H3BO3      24
KI         1.8
MnSO4      20
CuSO4      10
Na2MoO4    1.5
CoCl2      1.5
FeCl3      1.23

DasGip Fermentation:

Strains were tested in small scale fermentation using the DasGip system in the industrially relevant medium containing detoxified hydrolysate and sucrose. Strains were propagated as described above; DasGip inoculation was performed using the following protocol:

Cell dry weight of SEED 2 was assessed based on the final optical density. Cell dry weight and optical density (600 nm) correlation was used to estimate the volume of the SEED 2 culture needed for fermentation. Targeted inoculation level was about 7% v/v; about 1.5 g/L cell dry weight. Appropriate volume of SEED 2 culture was harvested by centrifugation (about 5000 rpm for 10 min) to pellet the cells and resuspended in about 17.5 ml of PBS. Resuspended cell solution was used to inoculate a 500 ml DasGip unit containing about 250 ml of detoxified hydrolysate and nutrient solution (about 3.5 ml/100 ml of medium). Fermentation was performed at about 32° C. at pH 6.3 with about 200 rpm. The duration of fermentation was about 92 hours with regular sampling. Sampling was conducted by a 25 ml steriological pipette through the port in the head plate of the DasGip unit. About 3 ml of culture were taken out, harvested by centrifugation (about 5000 rpm for 10 min) to pellet the cells and the supernatant was submitted for analysis. Standard analytical techniques such as high-pressure liquid chromatography (HPLC) were used to determine concentration of sugars and ethanol in the medium. Fermentation performances for yeast strains BD31378 (expressing a xylose isomerase of SEQ ID NO:96) and BD31365 (expressing a xylose isomerase of SEQ ID NO:78) are presented in FIG. 7A and FIG. 7B, respectively.

While various specific embodiments have been illustrated and described, it will be appreciated that various changes can be made without departing from the spirit and scope of the invention(s).

Claims

1. An isolated nucleic acid sequence which encodes a polypeptide comprising an amino acid sequence having at least 95%, 97% or 98% sequence identity to amino acid residues 2-377 of SEQ ID NO:96, wherein the nucleic acid encodes a polypeptide with at least one substitution relative to SEQ ID NO: 96 or at least one heterologous amino acid flanking the N-terminal or the C-terminal.

2. An isolated nucleic acid sequence having at least 90%, 93%, 95%, 96%, 98%, or 99% sequence identity, or having 100% sequence identity, to the nucleotide sequence of SEQ ID NO: 95, or to a portion thereof and encoding a xylose isomerase catalytic or dimerization domain, wherein the nucleic acid comprises at least one substitution relative to SEQ ID NO: 95 or is codon optimized.

3. A vector comprising the nucleic acid sequence of claim 2.

4. The vector of claim 3 which further comprises an origin of replication.

5. The vector of claim 3, which further comprises a promoter sequence operably linked to said nucleic acid sequence.

6. The vector of claim 5, wherein the promoter sequence is operable in yeast.

7. The vector of claim 5, wherein the promoter sequence is operable in filamentous fungi.

8. The nucleic acid sequence of claim 1 or 2, further comprising the amino acid sequence of (a) SEQ ID NO:21.2 or SEQ ID NO:213 and/or (b) SEQ ID NO:214.

9. The nucleic acid sequence of claim 8, wherein the encoded polypeptide comprises an amino acid sequence having at least 95%, 97% or 98% sequence identity to amino acids 2-377 of SEQ ID NO:96.

10. The nucleic acid sequence of claim 8 or 9, wherein the encoded polypeptide comprises an amino acid sequence having at least 95%, 97% or 98% sequence identity to SEQ ID NO:96.

11. A host cell transformed with the vector of claim 3.

12. The host cell of claim 11 which is a prokaryotic cell.

13. The host cell of claim 12 which is a bacterial cell.

14. The host cell of claim 11 which is a eukaryotic cell.

15. A recombinant cell engineered to express the polypeptide encoded by the nucleic acid sequence of any one of claims 1, 2, 8, 9 or 10.

16. The recombinant cell of claim 15 which is a eukaryotic cell.

17. The recombinant cell of claim 15 which is a yeast cell.

18. The recombinant cell of claim 17 which is a yeast cell of the genus Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Klockera, Schwanniomyces, Issatchenkia or Yarrowia.

19. The recombinant cell of claim 18, wherein the yeast cell is of the species S. cerevisiae, S. bulderi, S. barnetti, S. exiguus, S. uvarum, S. diastaticus, K. lactis, K. marxianus or K. fragili, or Issatchenkia orientalis.

20. The recombinant cell of claim 19, which is a S. cerevisiae cell.

21. The recombinant cell of claim 20, comprising one or more genetic modifications resulting in at least one of the following phenotypes: (a) an increase in transport of xylose into the cell;(b) an increase in xylulose kinase activity;(c) an increase in aerobic growth rate on xylose;(d) an increase in flux through the pentose phosphate pathway into glycolysis;(e) a decrease in aldose reductase activity;(f) a decrease in sensitivity to catabolite repression;(g) an increase in tolerance to ethanol, intermediates, osmolarity or organic acids; and(h) a reduced production of byproducts.

22. The recombinant cell of claim 21, wherein one or more genetic modifications result in increased expression levels of one or more of a hexose or pentose transporter, a xylulose kinase, an enzyme from the pentose phosphate pathway, a glycolytic enzyme and an ethanologenic enzyme.

23. The recombinant cell of claim 22, wherein the increased expression levels are achieved by overexpressing an endogenous gene or expressing a heterologous gene in the recombinant cell.

24. The recombinant cell of claim 21, wherein one or more genetic modifications result in decreased expression levels of one or more of a hexose kinase gene, the MIGI gene, and the MIG2 genes.

25. The recombinant cell of claim 21 which is engineered to express the xylose reductase (XR), xylose kinase (XK) and xylitol dehydrogenase (XD) pathway.

26. The recombinant cell of claim 17 in which the nucleic acid is operably linked to a promoter that is insensitive to catabolite repression.

27. The recombinant cell of claim 26 in which the promoter is the TDH3 promoter, the PGK1 promoter, the TEF1 promoter, or the ADH1 promoter.

28. The recombinant cell of claim 15 which is a filamentous fungal cell.

29. The recombinant cell of claim 28, wherein the filamentous fungal cell is of the genus Aspergillus, Penicillium, Rhizopus, Chrysosporium, Myceliophthora, Trichoderma, Humicola, Acremonium or Fusarium.

30. The recombinant cell of claim 28, wherein the filamentous fungal cell is of the species Aspergillus niger, Aspergillus oryzae, Trichoderma reesei, Penicillium chrysogenum, Myceliophthora thermophila, or Rhizopus oryzae.

31. A method for producing a fermentation product, comprising culturing the recombinant cell of claim 15 in medium containing xylose under conditions in which the fermentation product is expressed.

32. The method of claim 31, wherein the xylose in the medium is provided by lignocellulosic hydrolysate.

33. The method of claim 31 or claim 32, wherein the fermentation product is ethanol.

34. The method of claim 31 or claim 32, wherein the fermentation product is butanol, diesel, lactic acid, 3-hydroxy-propionic acid, acrylic acid, acetic acid, succinic acid, citric acid, malic acid, fumaric acid, itaconic acid, an amino acid, 1,3-propane-diol, ethylene, glycerol, a β-lactam antibiotic or a cephalosporin.

35. The method of claim 34, wherein the recombinant cell comprises a genetic modification that results in decreased alcohol dehydrogenase activity.

36. The method of claim 34 or claim 35, wherein the recombinant cell expresses one or more enzymes that confers on the cell the ability to produce said fermentation product.

37. The method of any one of claims 31 to 36, wherein the medium further comprises glucose.

38. The method of any One of claims 31 to 36, wherein the recombinant cell is cultured under anaerobic conditions.

39. The method of any one of claims 31 to 38, further comprising recovering the fermentation product.