Patent Application
20210147493
This application relates to processes for creating zein-enriched and zein-depleted corn protein fractions.
There is a long history of process development related to isolation of zein for a variety of industrial uses. Zein isolation typically involves dissolution of the zein protein from corn gluten meal using aqueous ethanol and subsequent solvent and “contaminant” removal, where a major portion of the non-protein composition of the zein is lipid. Variations on this process have been developed, but always starting from corn gluten meal. While there are commercial zein producers, the zein is expensive and therefore typically unsuited to food use. In addition, the zein-depleted fraction does not seem to be used in foods. Thus two potentially high-protein ingredients are not available to food formulators.
Described herein is a corn protein product, comprising a first fraction comprising 75 wt % to 95 wt % (dry solids) protein and a second fraction comprising 60 wt % to 80 wt % (dry solids) protein, wherein the first fraction is a zein-enriched fraction and the second fraction is a zein-depleted fraction and a method of achieving the same. Further described herein is a corn protein product derived from destarched corn gluten meal comprising a first fraction comprising 78 wt % to 83 wt % (dry solids) protein and a second fraction comprising 70 wt % to 80 wt % (dry solids) protein, wherein the first fraction is a zein-enriched fraction and the second fraction is a zein-depleted fraction.
A process by which the starch in corn gluten meal is removed yielding a product with about 75% protein is disclosed in U.S. Pat. No. 9,226,515, which has been further processed to remove lipid and pigments and which has greater than 85% protein as disclosed in International Application No. PCT/2016/024020. In this process, the objective is to maximize protein yield, so conditions that minimize protein solubilization are identified and employed. The process described is also quite cost-effective.
Aspects of the invention described herein explore whether using high-water solvents might yield a novel fractionation of the corn protein. For example, the ratio of zein dissolved to total protein might vary depending on whether corn gluten meal (“CGM”) or destarched (according to U.S. Pat. No. 9,226,515 and hereinafter referred to as Empyreal®) CGM is used. Similarly, there may be other proteins that are preferentially dissolved or retained as a consequence of prior heat and enzyme treatment.
The processing history of Empyreal® creates material that responds differently to ethanol extraction than corn gluten meal. This difference impacts yield, first extract purity, as well as protein and amino acid distribution. It has been surprising that the zein-enriched protein extracted from Empyreal® is not the same as the zein-enriched material extracted from CGM.
In practical terms, there is a tension between the technical properties of a material that tends to drive towards higher purity and the economic consequences of seeking high purity. A focus and intention in this technology development is on the less pure protein with attention towards lower cost. In these terms, the benefits of using Empyreal® rather than CGM as a raw material become salient. It has been found that the extracted residue from Empyreal® is close to 70% protein on a dry basis compared to about 40% from CGM (both values rise slightly after further defatting). This has a huge impact on the value of the two protein fractions as with protein under 50%, feed is the most likely outcome. With protein over 70%, food is a more likely and valuable use.
Disclosed herein is a corn protein product comprising a zein-enriched fraction (also referred to herein as a “first fraction” and an “extract”) and a zein-depleted fraction (also referred to herein as a “second fraction” and a “residue). Surprisingly herein, the zein-enriched fraction comprises 75 wt % to 95 wt % (dry solids) protein and the zein-depleted fraction comprises 60 wt % to 80 wt % (dry solids) protein. Further, in some aspects the zein-enriched fraction comprises 78 wt % to 83 wt % (dry solids) protein and the zein-depleted fraction comprises 70 wt % to 80 wt %. Unexpectedly, the zein-depleted fraction comprises a high enough protein content that it too (along with the zein-enriched fraction) can be used for food applications.
To achieve such a corn product, aspects of the present invention start with a destarched corn gluten meal, for example but not limited to Empyreal®. It has been found that destarched corn gluten meal, when compared to corn gluten meal, achieves a zein-depleted fraction higher in protein than corn gluten meal. Separation of the zein-enriched and zein-depleted fractions is achieved using an aqueous solution of an alcohol like ethanol or isopropanol. In preferred aspects, the organic solvent is an ethanol-water solvent comprising 55-80 wt % ethanol. Once the organic solvent is added to the destarched corn gluten meal, a series of solids-liquid separations (such as filtration or centrifugation) and homogenization techniques commonly known to one skilled in the art are carried out to recover a zein-enriched and zein-depleted fraction. Notably, higher ethanol concentrations increase the amount of protein present in the zein-depleted fraction. Furthermore, results suggest that using destarched corn gluten meal yields a purer protein in the zein-enriched fraction than corn gluten meal and the zein-depleted fraction can have up to 1.75 times higher protein concentration.
Also notable is that the zein-enriched and zein-depleted fractions have different amino acid distributions depending on whether corn gluten meal or Empyreal® is used as starting material, suggesting that the protein compositions of the fractions are not identical. Further, the zein-enriched and zein-depleted fractions have different fatty acid profiles.
Furthermore, the percent yield of protein is also interesting. In preferred aspects, the zein-enriched fraction is at least 50%, and more preferably at least 55% of the protein present in the starting material. In preferred aspects, the zein-depleted fraction is at least 30%, and more preferably at least 40% of the protein present in the starting material. It shall be recognized that there may some protein loss in the process. Furthermore, an optional defatting step improves the protein purity in the zein-depleted fraction. The zein-enriched and zein-depleted fractions are treated with organic solvents such as ethanol, hexane, and ethyl acetate to remove lipids and pigments.
The following materials and methods were used in the remaining examples.
CGM and Empyreal were collected as wet cakes at the Cargill Starch and Sweeteners corn wet mill in Blair, Nebr. Cakes were frozen, transported and stored frozen until shortly before use. The Empyreal® wet cake was treated with about 200 ppm H2O2 to oxidize free sulfite but the CGM was not.
Protein analysis was conducted using a Leco FP628 machine following the manufacturer's directions and using EDTA as a standard. Protein is calculated as 6.25×N.
For determining the solids content of raw materials, duplicate or triplicate samples were dried on a Sartorius moisture balance. For determining the solids content of final samples, approximately 1 g of cake or 10 g of extractant were weighed into tared aluminum dishes and left to dry at 80-100° C. for at least overnight under vacuum.
This example shows that CGM and Empyreal® have different fractionation characteristics from each other and yield different proximate residue compositions. 10 g CGM of Empyreal cake is weighed into a 50 mL centrifuge tube. Extractant is prepared according to Table 1 and added to the tube.
The tubes are shaken by hand and then the contents are mixed with a handheld homogenizer. The tubes are then placed in a 60° C. water bath. Tubes are shaken periodically and after 30 minutes, the tubes are centrifuged at 10000 rpm for 2 minutes. The supernatant is poured into a separate tube and an additional 10 g of extractant is added and re-homogenized. After an additional 30 minutes at 60° C., the supernatant is collected again after centrifugation at 10000 rpm. Supernatants are combined.
The cake is put into an A1 pan and weighed, then dried in a vacuum oven. The filtrates are combined and weighed. About 10 g of the filtrate is placed in a tared dish, air-dried partially and placed in a vacuum oven to dry.
The maximum yield of extracted mass is found at about 65 wt % EtOH (
When Empyreal® is extracted, the extracted solids (residue) showed a generally inverse behavior with the minima of residual solids around 65 wt % EtOH (
Extracts derived from Empyreal and CGM have similar concentrations of protein in their dry solids (
A representative sample of the extract and residue from a fractionation at 60° C. and 67 wt % EtOH described in Example 1 is submitted to SDS gel electrophoresis. Based on the protein concentration, samples are weighed out containing a calculated 28 mg of protein and mixed with 10 mL of 0.1N NaOH containing 1 wt % SDS. This is left overnight to hydrate and dissolve. An aliquot containing 100 μL of Laemmli buffer containing 1 mM dithiothreitol is added to 100 μL of the alkaline extract and exposed to a boiling water bath for 5 minutes. The sample is cooled and centrifuged at 13000 g for 5 minutes to remove particulates. A 20 μL aliquot is loaded into the wells of an AnykD™ Mini-PROTEAN® TGX™ Precast Gel and resolved on the Mini-PROTEAN® system. Gels are run until the marker dye reaches the bottom of the gel. Gels are stained with Bio-Safe™ Coomassie Brilliant Blue G-250 and destained in water.
Each of the samples (CGM and Empyreal® extracts (“zein-enriched”)) are loaded 4 times to provide opportunity for quantitative assessment. Images of destained gels are created using Licor Odessey scanner using Image Studio version 2.0 software. Quantitation is done with the manual analysis in Image Studio version 2.0 software.
The extract samples produced relatively clear bands which permitted quantitative analysis. Zein forms a triplet of bands at about 25 kDa that dominates the profile. The triplet comprises about 76% of the extract from Empyreal® but about 57% of the extract from CGM. These are statistically different (p=0.003) using a 2-sample T-test.
A second band is also visible at about 40 kDa. This band is almost twice as prominent in the Empyreal® extract (2.48%) compared to the CGM extract (1.0%) which is also statistically different (p=0.016). This comparison is shown in
Taken together, the results of Examples 1 and 2 suggest that using Empyreal® as a starting material actually yields a purer protein in the extraction than using CGM and the residual material is 1.75-times higher protein concentration. It is logical to infer that the distribution of proteins in the residue is also different as a function of the starting materials.
The same procedure in Example 1 is used to prepare a zein-enriched extract and a zein-depleted residue from Empyreal® and CGM wet cakes. The Empyreal cake is 39.7% solids and the CGM cake is 38.9% solids. 240 g of 69 wt % EtOH is added to 100 g of thawed cake and homogenized with a hand-held homogenizer. The mixture is placed at 60° C. for 30 minutes with periodic shaking. Extract is recovered by filtration on a Buchner funnel with 18.5 cm Whatman 113 filter paper. The residue is resuspended in 250 g of 66 wt % EtOH and the extraction and filtration is repeated. The solids are resuspended a third time in 250 g of 66 wt % EtOH and the separation repeated.
The final residue is air dried. The extracts are combined and concentrated by a combination of air drying and drying under a Na stream. After sufficient solvent is removed, the protein coagulated and the solvent is poured off. The remaining mass completed air drying.
Amino acid analysis is conducted after overnight hydrolysis of samples in 6N HCl under vacuum at 110° C. After cooling, samples are neutralized with 6N KOH. The primary amino acids are derivatized in 2% diethyl ethoxymethylenemalonate in methanol solution and 1M cesium bicarbonate buffer. A 1 μL aliquot is injected onto a Waters Acquity CORTECS reversed phase C18 column (100×2.1 mm, 1.6 um) installed in an Agilent 1290 ultra-high performance liquid chromatography instrument. The derivatized amino acids are eluted by a linear gradient comprising 95% mobile phase A (20 mM ammonium formate with 0.1% formic acid) to 95% mobile phase B (acetonitrile). The analytes are then detected by UV absorbance at 282 nm. Peaks are quantified by comparison to a standard obtained from Sigma (A9781) Amino acid analyses are reported as the percent of each amino acid as a fraction of all amino acids detected.
Two comparisons are of primary interest. Table 2 shows that the amino acid distribution in the extract from Empyreal® was dissimilar to that of the extract derived from CGM.
The compositions of the two extracts is compared by dividing the Empyreal®-derived amino acid concentration by the CGM-derived concentration for each amino acid. Empyreal® extract protein is relatively low in ammonia (derived from glutamine and asparagine), and glycine. Empyreal®-derived extract is enriched in aspartic acid, isoleucine and especially lysine.
A similar analysis of the residue after extraction (Table 3) shows that Empyreal® residue is relatively depleted in ammonium, arginine, aspartic acid, glycine, histidine, lysine, and valine.
Notably, the residue comprises greater than 11.5% leucine, greater than 5.0% tyrosine, and less than 4.0% lysine on a protein basis
The Empyreal®-derived fraction is enriched in glutamic acid, leucine and tyrosine
Without being bound to any particular theory, the consequences of the differing amino acid compositions is not obvious, but it strongly suggests that the protein compositions of the fractions are not identical. Identical protein compositions would have identical amino acid compositions. Shifting proportions of a set of proteins could lead to shifting amino acid distributions. The results of the SDS gel electrophoresis do suggest a changing proportion of constituent proteins. The amino acid analyses are consistent with that observation.
A 250 g sample of thawed Empyreal® cake (62.77% moisture) is placed in a 1000 mL bottle. Ethanol (424 g) and water (70 g) are added to the bottle and the mixture is homogenized with a handheld homogenizer. The bottle is placed in a 60° C. water bath and agitated periodically over a 30 minute period. The solids are recovered by filtration of the mixture through a sheet of 18.5 cm Whatman 113 filter paper. The filter cake is resuspended in 300 g of 66 wt % EtOH and returned to the water bath for 30 min with periodic shaking. The filter cake is partially collected on filter paper as before. Filtration is poor, so unfiltered suspension is centrifuged at 6000 rpm (approx. 5500 g) for 3 minutes. The pellet is combined with the filter cake and resuspended in 300 g of 66 wt % EtOH. The bottle is returned to the water bath for 30 minutes with periodic shaking. Solids are recovered by centrifugation as before. The solids are resuspended and the entire process repeated like the previous step.
The solids are recovered from the centrifuge bottles and broken into small pieces which are placed in an aluminum tray to dry under partial vacuum under warm conditions. The filtrates and supernatants are combined and concentrated by rotary evaporation at 50° C. and 24 in vacuum. When sufficient EtOH is removed, the protein agglomerates and evaporation is stopped. The protein settles and a water-rich fluid is poured off of the jelly-like mass. The soft mass is placed in an aluminum tray and dried under vacuum under warm conditions. The dried extract solids comprises about 93 wt % protein and the dried residue solids comprises about 79 wt % protein, both on a dry basis.
Samples of zein-enriched extract solids and zein-depleted residue solids are defatted by serial extractions with organic solvents: absolute ethanol, hexane, and ethyl acetate. One gram samples of dry ground extract and residue are placed in 15 mL plastic centrifuge tubes. To each tube, 3 mL of solvent is added and mixed. Samples are incubated for 10 minutes with constant inversion at room temperature. The solvent is recovered by centrifuging the samples for 3 minutes at 2000 g and then lifting off the free liquid with a pipette. The extraction is repeated 3 additional times for a total of 4 extractions. Samples are dried under a Na stream overnight. The lipid content of the fractions are measured using AOCS Ce-1h-05.
The lipid contents of fractions in this example are shown in Table 4.
The residue sample has much higher total fat, and each subtype of fat concentration than the extract. Without being bound to any particular theory, this probably reflects the poor lipid dissolving power of the aqueous EtOH containing 35 wt % water. Further extraction with solvents containing minimal water decreased the total fatty acid concentration further. The results also indicate that not all solvents are equally effective in removing the lipids present in these two protein fractions.
The bulk zein-depleted sample was defatted by first grinding the protein in a coffee grinder until the entire sample passed through a 425 μm screen. 25 g material and 125 g absolute ethanol were weighed into five, 250 mL polyethylene bottles. Bottles were hand shaken and placed in a water bath set to 40° C. The bottles were periodically hand shaken throughout the 30 minute heating period and, when removed from the water bath, centrifuged at 9000 rpm for 5 minutes. The supernatant was poured off and 75 g ethanol added to each bottle containing the remaining solids. Solids were re-suspended by hand shaking and the heating and centrifuging was repeated. The first and second defatting extractions were time consuming and inefficient so it was decided that the samples should be filtered instead of centrifuged. The third and fourth defatting extractions were conducted by adding 50 g absolute ethanol to the remaining solids, shaking, and heating in the water bath as above. After 30 minutes, the bottles were shaken to re-suspend the material and filtered using a Buchner funnel and Whatman 1 filter paper. Solids were transferred to an aluminum pan and dried in a vacuum oven set to 40° C. overnight. Results of fat content are determined using the AOCS Ce-1h-05 method
To obtain the defatted zein-enriched samples, it shall be understood that additional agitation is required. Results are shown in Table 5.
In this example, the defatted zein-depleted fraction demonstrates total fat content less than 1.5 wt % (db).
One hundred grams of corn protein isolate (note that corn protein isolate is used because its fractionation is similar to that of Empyreal® and to illustrate defatting prior to fractionation) prepared according to PCT/US2016/024020 is placed in a 1 L plastic bottle. The loss on drying is 7.57% and the protein is 88.4% on a dry basis. Aqueous ethanol (65 wt %) is added and the suspension is homogenized then placed at 50° C. with periodic mixing for 75 minutes. Solids are collected by filtration on VWR 417 paper on a Buchner funnel. The solids are resuspended in 400 g of 65 wt % EtOH and the incubation at 50° C. continued for 30 minutes. The solids are collected again as described above, and resuspended in 400 g of 65 wt % EtOH for 30 minutes at 50° C. The solids are collected as described above and placed in an aluminum pan to dry in an oven; this represents the residue. The filtrates are combined and held at room temperature until concentration began. The combined filtrates are concentrated in a rotary evaporator to remove about half the solvent. The concentrate is stored at about 4° C. for three days during which time a soft precipitate forms. The pellet is collected by centrifugation (3000 g, 5 minutes, ambient temperature). Further concentration did not precipitate more material and the liquid is evaporated to yield a solid material. Protein and loss on drying is analyzed for the recovered extract and residue fractions. About 15.5% of the initial protein is unaccounted for. About 45% of the protein recovered was in the extract. Table 6 shows the results.
Four hundred grams of corn protein isolate is prepared according to PCT PCT/US2016/024020, but collected before solvent removal, is placed in a 1 L plastic bottle. The loss on drying is 75% and the protein is 84.3% on a dry basis. The solvent composition at this point is between 98 and 100 wt % EtOH. Deionized water (161 g) is added and the suspension is homogenized then placed at 50° C. with periodic mixing for 30 minutes. Solids are collected by filtration on Whatman 113 paper on a Buchner funnel. The solids are resuspended in 260 g absolute EtOH plus 140 g of deionized water and the incubation at 50° C. continued for 30 minutes. The solids are collected again as described above, and resuspended in 260 g absolute EtOH plus 140 g of deionized water for 30 minutes at 50° C. The solids are collected as described above and suspended briefly in absolute EtOH before a final filtration and solids recovery.
The solids are placed in an aluminum pan to dry in an oven. This represents the residue of extraction. The filtrates are combined and held at room temperature until concentration begins. The combined filtrates are concentrated in a rotary evaporator to remove about two-thirds the solvent. The concentrate is stored at about 4° C. for five days during which time a soft precipitate forms. The pellet is collected by filtration as described above. Protein and loss on drying is analyzed for the recovered extract and residue fractions. About 57% of the protein recovered is in the extract. An indication of the variability in these analyses is shown by the fact that the total recovered protein exceeds the initial protein. Table 7 shows the results.
A 10 g sample of wet destarched corn gluten meal cake is weighed into a 50 mL centrifuge tube. NaOH (0.11 g of 1M solution) is added to adjust the pH to approximately 6.0. Twenty-four grams of 24 g of 89 wt % EtOH extractant is prepared and added to the cake. Cake and extractant is shaken then homogenized using a handheld mixer and placed in a 60° C. water bath with periodic mixing over 30 minutes. The suspension is centrifuged at 10000 rpm (TA10.25i rotor) for 2 minutes. The extract was decanted to a separate tube. Ten grams of 65 wt % EtOH was added to the tube, remixed and the incubation at 60° C. continued from another 15 minutes. Solids and liquids were separated by centrifugation as above. The resulting cake was placed in an aluminum pan and dried in a vacuum oven. The filtrates were combined and weighed. About 10 g of filtrate was placed in a pre-weighed dish, air-dried to remove a substantial fraction of the solvent and then dried in a vacuum oven. Loss on drying and protein concentration were determined on both. About 53% of the protein was found in the extract. Results are shown in Table 8.
Comparison of previous examples indicate that defatting has minimal impact on subsequent fraction of protein into the extract. Similarly, fractionation before and after solvent removal is similar.
A 10 g sample of wet corn gluten meal cake is weighed into a 50 mL centrifuge tube. NaOH (0.326 g of 1M solution) is added to adjust the pH to approximately 6.0 similar to the pH of Empyreal®. Twenty-four grams of 24 g of 89 wt % EtOH extractant is prepared and added to the cake. Cake and extractant is shaken then homogenized using a handheld mixer and placed in a 60° C. water bath with periodic mixing over 30 minutes. The suspension is centrifuged at 10000 rpm (TA10.25i rotor) for 2 minutes. The extract is decanted to a separate tube. Ten grams of 65 wt % EtOH is added to the tube, remixed and the incubation at 60° C. continues for another 15 minutes. Solids and liquids are separated by centrifugation as above. The resulting cake is placed in an aluminum pan and dried in a vacuum oven. The filtrates are combined and weighed. About 10 g of filtrate is placed in a pre-weighed dish, air-dried to remove a substantial fraction of the solvent and then dried in a vacuum oven. Loss on drying and protein concentration are determined on both. About 70% of the protein is found in the extract. Results are shown in Table 9.
A 40 mg sample of the zein-depleted material matching the zein-enriched sample in Example 2 and derived from destarched corn gluten meal derived from Example 1 is weighed into a 2 mL microcentrifuge tube. A 60 mg sample of zein-depleted material matching the zein-depleted sample in Example 2 and derived from corn gluten meal from Example 1 was weighed into a 2 mL microcentrifuge tube. The intent is to equalize the ultimate protein concentrations loaded onto the gels. Two millimeters of absolute EtOH is added to each tube and mixed vigorously then placed at 55° C. for 2.5 hour to remove lipid materials. Solids and liquids are separated by centrifugation for 3 minutes at 13,000 g. The solvent is lifted off with a pipette. 2 mL of a 50:50 w/w mixture of EtOH:ethyl acetate is added to each tube and incubated for 30 minutes at 55° C. Solids and liquids are separated by centrifugation for 3 minutes at 13,000 g. The solvent is lifted off with a pipette. Samples are allowed to evaporate to dryness in a hood.
1000 microliters of 6M urea plus 100 microliters of 1N NaOH is added to each sample, mixed on a shaker for 1 hr at 1000 rpm and then left to incubate at room temperature to hydrate and dissolve. An aliquot containing 100 μL of Laemmli buffer containing 1 mM dithiothreitol is added to 100 μL of the alkaline-urea extract and exposed to a boiling water bath for 5 minutes. The sample is cooled and centrifuged at 13000 g for 5 minutes to remove particulates. A 20 μL aliquot is loaded into the wells of an AnykD™ Mini-PROTEAN® TGX™ Precast Gel and resolved on the Mini-PROTEAN® system. Gels are run until the marker dye reaches the bottom of the gel. Gels are stained with Bio-Safe™ Coomassie Brilliant Blue G-250 and destained in water. Destained gels are scanned and quantitated. The proportion of total signal associated with various MW bands is compared and demonstrated in Table 10 and
To test the zein-depleted protein in the beef frank application, ingredients listed in Table 11 are weighed into disposable plastic containers and stored in the refrigerator until use. The control and the zein-depleted beef frank are prepared in duplicate by two analysts using a Cuisinart food processor with the blade attachment. One control and one sample frank is prepared by each analyst.
The control is prepared by adding the ground beef, salt, water (1st and 2nd deionized water are combined), and lard to the Cuisinart bowl. The mixer is quick-pulsed 5 times between each ingredient addition. After adding the lard, the mixer is quick-pulsed and then run at steady power for 1 minute. After the initial mix, the cover is removed and a rubber scraper used to scrape down the lid, bowl sides, and under the blade to ensure all ingredients are homogenized. The mixer is again run at steady power for 1 minute. 30 g of the mixture is weighed, in duplicate, into two tared 50 mL centrifuge tubes. The procedure is repeated by the second analyst using a clean/dry Cuisinart bowl for a total of 4 controls.
The zein-depleted beef frank is prepared by adding the protein to the 1st deionized (25 g) water in a 50 mL centrifuge tube. The tube is hand shaken and set aside for initial equilibration. The beef and salt are added to a clean/dry Cuisinart bowl, quick-pulsing 5 times after each addition. The water/protein suspension is poured into the bowl, and the tube is rinsed into the bowl using the 2nd (35 g) water. The mixer is pulsed 5 times and the lard added. The rest of the procedure is the same as the control.
The centrifuge tubes containing the controls and zein-depleted samples are centrifuged at 3000 g for 1 minute to uniformly pack the mixture in the tube. Tubes are then placed in a water bath set to 75° C. for 35 minutes. Once removed from the water bath, the liquid is drained using a spatula to free the meat frank from the tube. The frank is removed entirely from the tube and rolled on a paper towel until the surface appeared dry. The frank is weighed to determine yield and losses. The addition of zein-depleted protein in a beef frank application increases the product yield 8.5% as compared to the control which contained no plant protein as demonstrated in Table 12.
The zein-depleted protein is included in a nutrition bar model application comparing to soy protein isolate (Supro 620 lot #M310014220). A mixture of 427 g Clearsweet 43/43 syrup, 150 g Isoclear 55, and 100 g glycerol is prepared and the syrup is warmed in a saucepan on an oven top burner to 50° C. with continuous stirring. 18.5 g of the syrup is then weighed in a beaker and 6.25 g protein ingredient (soy or corn) is added and mixed with a stiff metal spatula until homogenous. Final composition of the bar is 45% sugar, 25% plant protein, and 15% water. Four soy protein samples are prepared. Two particle sizes of zein-depleted samples, <105 μm and >105 μm, are prepared in duplicate.
The homogenized mixture is poured into a 1 oz sample cup, covered, and tapped on the bench to remove any air. Samples are stored in a sealed box and the resistance to compression is measured 24-72 hours after the protein addition using a texture analyzer. Zein-depleted protein produced a softer bar than soy protein isolate as demonstrated in Table 13.
This application claims the benefit of U.S. Provisional Patent Application No. 62/561,931, filed Sep. 22, 2017, which is hereby incorporated by reference in its entirety
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2018/052153 | 9/21/2018 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 62561931 | Sep 2017 | US |
