Claims
- 1. An isolated nucleic acid molecule encoding a polypeptide wherein the encoded polypeptide comprises amino acid residues 18-459 of SEQ ID NO:2.
- 2. The isolated nucleic acid molecule of claim 1, wherein the encoded polypeptide further comprises an affinity tag.
- 3. The isolated nucleic acid molecule of claim 1, wherein the encoded polypeptide comprises amino acid residues 1-459 of SEQ ID NO:2.
- 4. The isolated nucleic acid molecule of claim 1, wherein the encoded polypeptide is SEQ ID NO:2.
- 5. A nucleic acid molecule encoding a fusion protein, wherein the encoded fusion protein comprises a first portion and a second portion joined by a peptide bond, wherein the first portion consists of amino acid residues 18-459 of SEQ ID NO:2; and wherein the second portion consists another polypeptide.
- 6. The nucleic acid molecule of claim 5, wherein the encoded fusion protein further comprises an affinity tag.
- 7. An expression vector comprising the following operably linked elements:
a transcription promoter; a DNA segment encoding a polypeptide wherein the encoded polypeptide comprises amino acid residues 18-459 of SEQ ID NO:2; and a transcription terminator.
- 8. The expression vector of claim 7 further comprising a secretory signal sequence operably linked to the DNA segment.
- 9. The expression vector of claim 8 further comprising an affinity tag operably linked to the DNA segment.
- 10. A cultured cell into which has been introduced an expression vector of claim 7, wherein the cell expresses the polypeptide encoded by the DNA segment.
- 11. A cultured cell into which has been introduced an expression vector of claim 8, wherein the cell expresses the polypeptide encoded by the DNA segment.
- 12. A method of producing a polypeptide comprising:
culturing a cell of claim 10; and isolating the polypeptide produced by the cell.
- 13. A method of producing a polypeptide comprising:
culturing a cell according to claim 11; and isolating the polypeptide produced by the cell.
REFERENCE TO RELATED APPLICATION
[0001] The present application is a continuation of U.S. patent application Ser. No. 09/997,610, filed Nov. 29, 2001, which claims the benefit of U.S. Patent Application Serial No. 60/253,924, filed on Nov. 29, 2000, both of which are herein incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60253924 |
Nov 2000 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09997610 |
Nov 2001 |
US |
Child |
10688570 |
Oct 2003 |
US |