Adjuvant for promoting absorption of therapeutically active substances through the digestive tract

This invention relates to an adjuvant for promoting absorption of therapeutically active substances through the digestive tract and a pharmaceutical composition comprising a therapeutically active substance and said adjuvant, said composition being beneficial in that the therapeutically active substance contained therein is readily absorbed into the blood stream through the digestive tract even when said active substance is usually absorbable through the digestive tract only with considerable difficulty.
Several compounds have heretofore been proposed as adjuvants for promoting absorption of medicinal substances through the digestive tract, but have been found to be disadvantageous in that some are useful only for oral application while others are not efficient enough to bring about a therapeutically effective blood concentration of the concomitant active substance.
The intensive search undertaken under the above circumstances into adjuvants which would promote absorption or therapeutically active substances through the digestive tract and particularly for those substances which could be successfully employed for rectal administration has resulted in the finding that enamine derivatives of the following general formula (I) have an excellent absorption-promoting action and that a pharmaceutical composition containing such an adjuvant is capable of ensuring a high and sustained blood concentration of the therapeutically active substance concomitantly present, irrespective of the kind of therapeutically active substance or the dosage forms employed, such as suppositories, tablets, capsules or sublingual tablets, etc.
Thus, this invention relates, in one aspect, to an absorption-promoting adjuvant comprising one or more members of enamine derivatives having the general formula: ##STR2## (wherein ##STR3## means the residue of an organic aminocarboxylic acid or organic aminosulfonic acid with a hydrogen atom removed from its amino group; the carboxyl or sulfo group of said residue being optionally in the form of an alkali metal salt or ester; R.sub.1 and R.sub.2 may as taken together form a ring structure; R.sub.3 is hydrogen atom, lower alkyl or lower alkoxy; R.sub.4 is hydrogen atom, lower alkyl or lower alkoxycarbonyl; R.sub.5 is lower alkyl, lower alkoxy which may optionally be substituted by hydroxyl, carboxyl or lower alkoxycarbonyl, or lower alkylamino substituted by hydroxyl, carboxyl or lower alkoxycarbonyl; When R.sub.3 and R.sub.4 are both lower alkyls, the two may as taken together form a carbocyclic group; when R.sub.4 is lower alkyl and R.sub.3 is lower alkoxy, the two groups may as taken together form an oxygen-containing ring.)
In another aspect, this invention relates to a pharmaceutical composition comprising a therapeutically active substance and said adjuvant.
It is therefore an object of this invention to provide an adjuvant which promotes absorption of a therapeutically active substance through the digestive tract, said adjuvant comprising of one or more enamine derivatives (I).
It is another object to provide a pharmaceutical composition comprising a therapeutically active component and said absorption-promoting adjuvant. Other objects of the invention will become apparent from the following description and claims.

FIGS. 1-7 are graphs showing the effectiveness of the present invention.
Some of the enamine derivatives (I) which may be employed in accordance with this invention are novel compounds. Thus, for example, the compounds represented by the following general formula: ##STR4##(wherein ##STR5##has the same meaning as defined hereinbefore; R'.sub.5 is a lower alkoxy orlower alkylamino group substituted by hydroxyl, carboxyl or lower alkoxycarbonyl) are novel compounds.
Referring to ##STR6##in general formula (I) and (I'), said organic aminocarboxylic acid means a carboxylic acid containing a primary or secondary amino group. Such organic aminocarboxylic acids include not only naturally-occurring alpha-amino acids but also such amino carboxylic acids as p-aminobenzoic acid, phenylglycine, etc. The naturally-occurring alpha-amino acids may beacidic, basic or neutral amino acids. Some di- and tripeptides also come under the definition of said organic aminocarboxylic acids. Thus, there may be mentioned aliphatic monoaminomonocarboxylic acids such as glycine, alanine, valine, leucine, isoleucine, etc.; aliphatic hydroxyamino acids such as serine, threonine, etc.; sulfur-containing aliphatic amino acids such as cysteine, cystine, methionine, etc.; aliphatic monoaminodicarboxylic acids such as aspartic acid, glutamic acid, etc. (inclusive of the compounds obtainable by amidating one of the carboxyl groups of such dicarboxylic acids, such as asparagine, glutamine, etc.); aliphatic diaminomonocarboxylic acids such as lysine, arginine, etc.; aromatic amino acids such as phenylalanine, tyrosine, etc.; heterocyclic amino acids such as histidine, tryptophan, proline, oxyproline, etc.; and so on. As examples of said amino carboxylic acids, which are not naturallyoccurring, there may be mentioned .beta.-aminopropionic acid, .gamma.-aminobutyric acid, anthranilic acid, p-aminobenzoic acid, etc. Thecarboxyl group of such organic aminocarboxylic acids may be in the form of an alkali metal salt (e.g. sodium, potassium or other salt) or an ester. The ester may for example be an alkyl ester of 1 to 6 carbon atoms, preferably one containing 1 to 4 carbon atoms (e.g. methyl, ethyl, propyl or other ester.)
The organic aminosulfonic acid means any of aliphatic, aromatic and heterocyclic sulfonic acids corresponding to said aminocarboxylic acids whose carboxyl groups have been replaced by a sulfo group.
Among such organic aminosulfonic groups are taurine and aminobenzenesulfonic acid. The sulfo group may be in the form of an alkalimetal salt or an ester as mentioned in connection with said carboxyl groups.
R.sub.1 and R.sub.2 are such that either one of R.sub.1 and R.sub.2 may forexample be a lower alkyl group or aryl group substituted by carboxyl and/orsulfo, and these groups may be further substituted by amino, mercapto, lower alkylamino, lower alkylthio, heterocyclic group, aryl group, etc. The other of R.sub.1 and R.sub.2 may usually be a hydrogen atom, although it may be lower alkyl, in which case R.sub.1 and R.sub.2 may as taken together form a ring structure.
In the above connection, the lower alkyl group may for example be an acyclic alkyl group of 1 to 6 carbon atoms, preferably 1 to 4 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, tert-butyl, pentyl, hexyl, etc.; and a cycloalkyl group of 5 to 6 carbon atoms, such as cyclopentyl and cyclohexyl. The aryl group may for example be phenyl, .alpha.-naphthyl or .beta.-naphthyl.
The lower alkyl group R.sub.3 in general formula (I) may for example be an acylic alkyl group of 1 to 6 carbon atoms, preferably 1 to 4 carbon atoms,such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, pentyl, hexyl, etc. or a cycloalkyl group of 5 to 6 carbon atoms, such as cyclopentyl and cyclohexyl. The lower alkoxy which is also designated by R.sub.3 may for example be an acyclic alkoxy group of 1 to 6 carbon atoms,preferably 1 to 4 carbon atoms, such as methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, tert-butoxy, pentyloxy, hexyloxy, etc. ora cycloalkoxy group of 5 to 6 carbon atoms, such as cyclopentyloxy, and cyclohexyloxy.
The lower alkyl R.sub.4 in general formula (I) may be any of the lower alkyls named with reference to R.sub.3. The lower alkoxy moiety of lower alkoxycarbonyl R.sub.4 may be any of the lower alkoxy groups mentioned with reference to R.sub.3.
The lower alkyl and lower alkoxy groups R.sub.5 in general formula (I) may be the same as those mentioned with reference to R.sub.3.
The above lower alkoxy group may be substituted by one or more members of the group consisting of hydroxyl, carboxyl and lower alkoxycarbonyl, etc. These substituents may be the same or different.
The lower alkoxy moiety of said lower alkoxycarbonyl group as one of the above-mentioned substituent may be the same as that defined and illustrated with reference to R.sub.3. The lower alkyl moiety of lower alkylamino substituted by hydroxyl, carboxyl and/or lower alkoxycarbonyl, as designated by R.sub.5, may be the same as that defined and illustrated with reference to R.sub.3. This lower alkylamino group may be substituted by 2 or more substituents which may be the same or different. The lower alkoxy moity of lower alkoxycarbonyl in said substituent may be the same as that defined and illustrated with reference to R.sub.3.
When both R.sub.3 and R.sub.4 are lower alkyls, the compound (I) may be represented by general formula (II): ##STR7##(wherein ##STR8##and R.sub.5 are as defined hereinbefore; R.sub.6 is lower alkylene)
The lower alklene group R.sub.6 may be an alkylene group of 1 to 4 carbon atoms, such as methylene, propylene, butylene, etc. When, in general formula (I), R.sub.4 is lower alkyl and R.sub.3 is lower alkyloxy, the compound (I) may be represented by the general formula (III): ##STR9##(wherein ##STR10##and R.sub.5 are as defined hereinbefore; R.sub.7 is lower alkylene)
The lower alkylene R.sub.7 may for example be an alkylene group of 1 to 4 carbon atoms, such as ethylene, propylene and butylene.
Referring to general formula (I'), the lower alkoxy or lower alkylamino group R'.sub.5 as substituted by hydroxyl, carboxyl and/or lower alkoxycarbonyl has the same meaning as defined with reference to R.sub.5 in general formula (I).
The enamine derivative (I) according to this invention can be produced by any procedure analogous to the method described in Chemiche Berichte, 98, 789 (1965). Thus, an organic aminocarboxylic acid, an aminosulfonic acid or an alkali metal salt thereof is reacted with an acylacetate or a .beta.-diketone at room temperature or an elevated temperature. This reaction may be written as follows. ##STR11##(In the above formulas, ##STR12##and R.sub.3 to R.sub.5 are as defined hereinbefore; R.sub.8 is a group which is removable on reaction with an amine.)
Thus, the primary or secondary amino group in said organic aminocarboxylic acid or aminosulfonic acid (IV) reacts with the acyl-acetate or .beta.-diketone (V) or a derivative thereof (VI) to yield the desired enamine derivative. As examples of compounds (V) and (VI), there may be mentioned acetylacetone, propionylacetone, butyroylacetone, methyl acetoacetate, ethyl acetoacetate, n-propyl acetoacetate, isopropyl acetoacetate, n-butyl acetoacetate, isobutyl acetoacetate, tert-butyl acetoacetate, n-pentyl acetoacetate, methoxyethyl acetoacetate, ethoxyethyl acetoacetate, isopropoxyethyl acetoacetate, n-propoxyethyl acetoacetate, .alpha.-acetylbutyrolactone, cyclopentanone-2-carboxylic acid methyl ester, cyclopentanone-2-carboxylic acid ethyl ester, cyclopentanone-2-carboxylic acid isopropyl ester, cyclopentanone-2-carboxylic acid n-butyl ester, cyclohexanone-2-carboxylicacid methyl ester, cyclohexanone-2-carboxylic acid ethyl ester, cyclohexanone-2-carboxylic acid propyl ester, ethoxymethylene malonic aciddiethyl ester, ethoxymethylene malonic acid dipropyl ester.
This reaction is generally carried out in a solvent. Solvents which will not interfere with the reaction may be employed and, as examples, isopropyl alcohol, ether, chloroform, dioxane, benzene, ethyl acetate and toluene may be mentioned. The reaction temperature may be a mild one, e.g.room temperature to a moderately elevated temperature.
As mentioned hereinbefore, the enamine derivative (I) is employed as an adjuvant for promoting absorption of therapeutically active substances through the digestive tract. The therapeutically active substances employed in accordance with this invention may be any substances or agentswhich display medicinal effects when absorbed through the digestive tract of the recipient and, therefore, may cover such varied therapeutic drugs as drugs affecting the central nurvous system, antimicrobial drugs, gastrointestinal drugs, drugs affecting metabolism, cardiovascular drugs, respiratory drugs, etc. More specifically, there may be mentioned .beta.-lactam antibiotics such as penicillins and cephalosporins, high molecular substances such as peptides, carbohydrates such as inulin, heparin, etc.. The peptides include insulin, urokinase, lysozyme and so on.
The penicillins include ampicillin, cyclacillin, cloxacillin, benzylpenicillin, carbenicillin, piperacillin, mezlocillin, pirdenicillin,ticarcillin and so on.
The cephalosporins include cephalexin cephalothin, cefoxitin, cefazolin, cephaloridine, cephacetrile, cefotiam, ceforanide and so on.
The pharmaceutical composition containing the absorption-promoting adjuvantaccording to this invention may assume any dosage form in which the therapeutically active substance thereof may pass into the circulation through the digestive tract. For example, oral preparations (e.g. capsules, tablets, etc.), rectal preparations (e.g. suppositories) and sublingual preparations are included. Particularly desirable are preparations for rectal administration.
Any of these dosage forms may be prepared by the established pharmaceuticalprocedures.
Taking rectal suppositories as an example, they may be manufactured in the following manner. First, the enamine derivative (I) is added to a suppository base and, then, a therapeutically active substance is added and dispersed. The resulting mixture is then filled and molded in a suppository container. The order of addition need not be limited to that described above but may be reversed. It is also possible to incorporate anantioxidant, preservative, volume-builder, etc. The suppository base just mentioned may be any known material, oleaginous or water-soluble. The oleaginous base includes, among others, such vegetable oils as peanut oil,olive oil, corn oil, fatty acid glyceride [e.g. Witepsol.RTM. (Dynamite Novel Chemicals), SB-Base.RTM. (Kanegafuchi Chemical Co., Ltd.), O.D.O.RTM. (Nisshin Oil K.K.), etc.] and such mineral oils as paraffin andvaseline.
The water-soluble base includes polyethylene glycol, propylene glycol, glycerin, etc.
The term `digestive tract` as used herein means any and all of the hollow organs of the body which digest and absorb and excrete the nutrients takeninto the body, such as oral cavity, esophagous, stomach, small and large intestines, rectum, etc. While therapeutically active substances are generally not well absorbed when rectally administered, the presence of the adjuvant of this invention in rectal doses assists considerably in theabsorption of therapeutically active substances. Therefore, this invention is especially valuable for application to rectal administration.
The level of addition of enamine derivative (I) is normally 0.5 to 85 weight %, preferably 1 to 20 W%, based on the whole preparation. More specifically, when the therapeutically active component is a .beta.-lactamantibiotic, the concentration of enamine derivative (I) may range from 1 to85 W%, preferably 2 to 20 W%, based on the whole preparation. In the case of insulin, the amount of enamine derivative (I) may range from 0.5 to 20 W%, preferably 1 to 15 W%.
In the case of the rectal preparations, the level of addition of therapeutically active substances is usually 0.0004 to 50 w% based on the whole preparation. More specifically, when the therapeutically active component is a .beta.-lactam antibiotic, the concentration of .beta.-lactam antibiotic may range from 2.5 to 50 w%, preferably from 5 to30 w%, based on the whole preparation. In the case of insulin, the concentration of insulin (24 units/mg) may range from 0.001 to 1 w%, preferably from 0.01 to 0.5 w%. In the case of heparin, the concentration of heparin Na (100 units/mg) may range from 0.1 to 50 w%, preferably from 0.5 to 30 w%. In the case of urokinase, the concentration of urokinase (210000 units/mg) may range from 0.0004 to 0.1 w%, preferably from 0.0005 to 0.01 w%.
In the case of the oral preparations, the level of addition of therapeutically active substances is 15-99 w%, preferably 80-98 w%, based on the whole preparation, when the therapeutically active substances are .beta.-lactam antibiotics.
Production Example 1 [Enamine derivative (I)]
The starting material aminocarboxylic acid or aminosulfonic acid sodium salt or methyl ester (0.2 M) (each of the material compounds mentioned in Table 1) was added to 50-100 ml of isopropyl alcohol, followed by additionof the acylacetate or .beta.-diketone (0.25 M) (also listed in Table 1). The mixture was stirred at room temperature or an elevated temperature until it became a clear solution. The insolubles were then filtered off, the solvent distilled off under reduced pressure and the residue washed with petroleum ether or diethyl ether. By the above procedure were obtained the enamine derivatives (I) having molecular formula shown in Table I, Compound Nos. 1 to 4, 6 to 8 and 10 to 17, (Table 1).
Production Example 2 [Enamine derivative (1)]
The starting material aminocarboxylic acid sodium salt or methyl ester (mentioned in Table 1) (0.2 M) was reacted with diethyl ethoxymethylenemalonate (0.3 M) in 200 ml of benzene under boiling, with the byproduct ethyl alcohol being azeotropically removed.
In 3 to 5 hours, there was obtained a pale yellow solution with small amounts of crystals suspended therein. Following the above reaction, the benzene was distilled off and the residue dissolved in methanol and filtered to remove the insolubles. Then, the methanol was distilled off and the residue washed with petroleum ether or diethyl ether. By the aboveprocedure were obtained enamine derivatives (I) having molecular formula shown in Table 1, Compound Nos. 5 and 9 (Table 1).
The elemental analyses, melting points and ultraviolet absorptions (.lambda.max, ethanol) are given in Table 2.
TABLE 1__________________________________________________________________________Starting materialsCompound Aminocarboxylic acid Acyl acetate or Molecular formula of productsNo. Aminosulfonic acid .beta.-diketone C H N O S Na__________________________________________________________________________1 Glycine (NA) Ethyl acetoacetate 8 12 1 4 0 12 .gamma.-Aminobutyric acid (Na) " 10 16 1 4 0 13 (D) Phenylglycine (Na) " 14 16 1 4 0 14 " Acetylacetone 13 14 1 3 0 15 " Ethoxy methylene malonic acid diethyl ester 16 18 1 6 0 1Acetylbutyrolactonealpha. 14 14 1 4 0 17 " Cyclopentanone-2- carboxylic acid butyl ester 18 22 1 4 0 18 (D) Phenylglycine Ethyl acetoacetate methyl ester 15 19 1 4 0 09 (D) Phenylglycine Ethoxy methylene malonic methyl ester acid diethyl ester 17 21 1 6 0 010 (DL) Phenylalanine Ethyl acetoacetate (Na) 15 18 1 4 0 111 (DL) Phenylalanine Acetylacetone 14 16 1 3 0 1 (Na)12 (DL) Phenylalanine .alpha.-Acetylbutyrolactone 15 16 1 4 0 1 (Na)13 (L) Proline (Na) Ethyl acetoacetate 11 16 1 4 0 114 Taurine (Na) " 8 14 1 5 1 115 Glycylglycine (Na) " 10 15 1 5 0 116 (L) Glutamic acid (Na) " 11 15 1 6 0 117 (L) Lysine (Na) " 18 29 1 6 0 1__________________________________________________________________________(Na): sodium salt
The chemical names of the obtained enamine derivatives (I) in Table 1 are as follows:
1. N-(1-Methyl-2-ethoxycarbonylvinyl)glycine sodium salt (Compound 1)
2. N-(1-Methyl-2-ethoxycarbonylvinyl)-.gamma.-aminobutyric acid sodium salt(Compound 2)
3. N-(1-Methyl-2-ethoxycarbonylvinyl)-D-phenylglycine sodium salt (Compound3)
4. N-(1-Methyl-2-acetylvinyl)-D-phenylglycine sodium salt (Compound 4)
5. N-[2,2-bis(ethoxycarbonyl)vinyl]-D-phenylglycine sodium salt (Compound 5)
6. N-[1-(Tetrahydro-2-furanone-3-ylidene)ethyl]-D-phenylglycine sodium salt(Compound 6)
7. N-(2-Butoxycarbonyl-1-cyclopenten-1-yl)-D-phenylglycine sodium salt (Compound 7)
8. Methyl-N-(1-methyl-2-ethoxycarbonylvinyl)-D-phenylglycinate (Compound 8)
9. Methyl-N-[2,2-bis(ethoxycarbonyl)vinyl]-D-phenylglycinate (Compound 9)
10. N-(1-Methyl-2-ethoxycarbonylvinyl)-DL-phenylalanine sodium salt (Compound 10)
11. N-(1-Methyl-2-acetylvinyl)-DL-phenylalanine sodium salt (Compound 11)
12. N-[1-(Tetrahydro-2-furanone-3-ylidene)ethyl]-DL-phenylglycine sodium salt (Compound 12) 13. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-proline sodiumsalt (Compound 13)
14. N-(1-Methyl-2-ethoxycarbonylvinyl)taurine sodium salt (Compound 14)
15. N-(1-Methyl-2-ethoxycarbonylvinyl)glycylglycine sodium salt (Compound 15)
16. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-glutamic acid sodium salt (Compound 16)
17. N,N'-Bis(1-methyl-2-ethoxycarbonylvinyl)-L-lysine sodium salt (Compound17)
TABLE 2______________________________________Com-pound Calculated (%) Found (%) mp .lambda. maxNo. C H N C H N (.degree.C.) (nm)______________________________________1 45.94 5.78 6.70 45.86 5.81 6.82 168-171 281.02 50.63 6.80 5.90 50.65 6.85 5.96 110-115 282.53 58.95 5.61 4.91 58.76 5.70 5.02 226-227 289.04 61.17 5.53 5.49 61.22 5.58 5.61 124-126 311.05 55.98 5.28 4.08 56.12 5.32 4.12 128-129 283.06 59.36 4.98 4.94 59.28 5.03 4.99 159-161 299.57 63.71 6.53 4.13 63.74 6.62 4.09 106-109 299.08 64.97 6.91 5.05 64.12 6.85 5.11 98-101 284.59 60.89 6.31 4.18 60.88 6.34 4.23 <-20 282.510 60.20 6.06 4.68 60.25 6.05 4.65 182-184 283.511 62.45 5.99 5.20 62.53 6.03 5.25 173-175 313.512 60.60 5.42 4.71 60.72 5.46 4.18 105-108 305.513 53.01 6.47 5.62 53.12 6.45 5.68 >250 289.514 37.06 5.44 5.40 37.03 5.40 5.43 113-115 285.515 45.98 5.79 10.72 45.94 5.69 10.82 129-132 278.016 43.57 5.00 4.62 43.52 4.98 4.70 239 (dec) 284.017 57.13 7.72 3.70 57.08 7.83 4.60 77- 9 285.5______________________________________
Stability in aqueous solution
To examine the stability in water of the enamine derivatives (I) obtained above, some representative compounds were selected and their half-lives [t0.5 (min.)] in pH 7.4 phosphate buffer (.mu.: 0.15, temp.: 25.degree. C.) were determined by ultraviolet absorption spectrometry. The results are presented in Table 3. In the Table, "stable" means that the enamine structure was retained even after 6 hours.
TABLE 3______________________________________Compound No. t 0.5 (min.) Compound No. t 0.5 (min.)______________________________________1 3.7 10 15.72 18.8 13 3.13 26.1 14 24.04 Stable 15 31.85 Stable 16 16.36 478 17 22.97 Stable______________________________________
EXAMPLE 1
______________________________________(Recipe 1: capsule)______________________________________Ampicillin (briefly, AB-PC) sodium 15 mg/kgCompound 3 10 mg/kg______________________________________
Capsules each containing the above indicated amounts of components were prepared by the conventional procedure and administrered to 3 rabbits. Immediately thereafter, each animal was given 20 ml of water and the concentration of AB-PC in the blood was measured by the biological assay method. The mean results are shown in FIG. 1. In the figure, white circlesrepresent AB-PC alone and black circles represent the capsule according to this invention. The absorption-promoting effect of compound 3 appeared 2 to 3 hours after dosing, and in terms of the area under the blood-concentration curve (AUC: .mu.g.min/ml), the results for each rabbitwere 1.52, 1.99 and 1.73, all in excess of 1.5 times of AB-PC alone.
The blood concentrations were measured in conformity with the Antibiotic Standard of Japan. The cup method was employed with Sarcina lutea.
EXAMPLE 2
______________________________________(Recipe 2: solution)______________________________________Compound 3 250 mgWater 20 ml______________________________________
An oral solution of the above composition was prepared according to the established pharmaceutical practice.
Capsules each containing 15 mg/kg of AB-PC sodium were orally administered to rabbits and immediately thereafter, the above solution was administeredalso by the oral route. The blood concentration of AB-PC (black circles) isshown in FIG. 2. The white circles represent AB-PC alone.
The blood concentrations were measured in conformity with the biological assay method in the Antibiotic Standard of Japan.
EXAMPLE 3
______________________________________(Recipe 3: rectal ointment)______________________________________CET-Na 20%Compound 3 10%Liquid paraffin 35%White vaseline 35%______________________________________
Cephalothin sodium (briefly CET-Na), a drug which is sparingly absorbed from the rectum, was selected for a test. Thus, CET-Na was dispersed in liquid paraffin-white vaseline base to prepare rectal ointment. The ointment was administered to rabbits by the rectal route. FIG. 3 shows thechange in blood concentration (dose: 50 mg CET-Na/kg). A high CET level in the blood could by very quickly achieved. The blood concentration was measured in conformity with the biological assay method in the Antibiotic Standard of Japan.
EXAMPLE 4
AB-PC and cephalexin (briefly, CEX), both of which are sparingly absorbed from the rectum, are also tested. Thus, enamine derivatives (I) and these antibiotics were used to prepare rectal ointments in accordance with established practice. The ointments were administered to rabbits to investigate the peak blood concentrations (.mu.g/ml) and AUC (.mu.g.min./ml). The results are shown in Tables 4, 5 and 6.
______________________________________(Recipes 4 to 7: rectal ointments)______________________________________ AB-PC . Na 10% Enamine derivative (I) 10% Liquid paraffin 40% White vaseline 40%Dose: 15mg AB-PC . Na/kg______________________________________
Compounds 3, 5, 13 and 14 were used as said enamine derivatives (I) in Recipes 4, 5, 6 and 7, respectively.
TABLE 4______________________________________ Peak bloodRecipe Enamine concentrationNo. derivative (I) (.mu.g/ml) AUC______________________________________4 Compound 3 4.8 2705 Compound 5 4.7 1496 Compound 13 2.6 1087 Compound 14 5.9 239______________________________________
______________________________________(Recipes 8 and 9: rectal ointments)______________________________________ CEX . H.sub.2 O 20% Enamine derivative (I) 10% Liquid paraffin 35% White vaseline 35%Dose: 50 mg CEX . H.sub.2 O/kg______________________________________
TABLE 5______________________________________ Peak bloodRecipe Enamine concentrationNo. derivative (I) (.mu.g/ml) AUC______________________________________8 Compound 2 6.7 2789 Compound 13 6.5 733______________________________________
______________________________________(Recipes 10-17: rectal ointments)______________________________________ CET . Na 20% Enamine derivative (I) 10% Liquid paraffin 35% White vaseline 35%Dose: 50mg CET . Na/kg______________________________________
TABLE 6______________________________________ Peak bloodRecipe Enamine concentrationNo. derivative (I) (.mu.g/ml) AUC______________________________________ 9 Compound 2 6.3 31510 Compound 3 14.4 44511 Compound 4 11.0 56812 Compound 5 6.1 30613 Compound 8 3.8 4914 Compound 13 2.5 2515 Compound 14 7.8 25016 Compound 15 1.8 10217 Compound 17 8.7 396______________________________________
EXAMPLE 5
The absorption-promoting effect of the enamine derivative (I) on .beta.-lactam antibiotics has been confirmed in the above experiments. Then, to examine similar effects on drugs having higher molecular weights,insulin which has a molecular weight of 6000 was selected.
______________________________________(Recipe 18: rectal suppository)Amorphous insulin 3 I.U.Compound 3 0.05gWitepsol H-15.RTM. 0.45g(Recipe 19: rectal suppository)Amorphous insulin 1 I.U.Compound 3 0.05gWitepsol H-15.RTM. 0.45g______________________________________
The blood glucose concentration observed after rectal administration of theabove suppository to rabbits (body weights: 2.0-2.5 kg) is presented in FIG. 4.
The test was conducted by the glucoseoxidase method. The broken line represents the control glucose level (insulin alone added to base). In this case, there has been no absorption of insulin. The white triangles represent the glucose level observed after administration of one I.U. of insulin per animal, while black triangles represent the level after administration of 3 I.U. of insulin per animal. Especially in the latter case, the blood glucose concentration is reduced about 50% from the pre-medication level. The largest depression of blood glucose from the pre-medication level was expressed in % response. The results are shown inFIG. 5. The dose response curve when insulin alone was intramuscularly administered is shown in FIG. 6. The absorption-promoting effect of the adjuvant of this invention was almost comparable to the effect of intramuscular injection.
EXAMPLE 6
______________________________________(Recipe 20 - rectal suppository)To investigate the versatility of the absorption-promoting action of the adjuvant according to this invention,inulin (mol. wt. 5000) was selected as a test polysaccharide.______________________________________Inulin 0.25gCompound 3 0.05gWitepsol H-15.RTM. 0.425g______________________________________
The above components were used to prepare a suppository in accordance with established pharmaceutical practice. The suppository was rectally administered to rabbits (body weights 2.0-2.5 kg) and the blood concentrations were determined.
The results, shown in FIG. 7, indicates the versatility of enamine derivative (I). The amount of inulin excreted in the urine was 18% of the dose administered.
The following dosage forms (dispersed in Witepsol H-15.RTM.) were rectally administered to dogs and humans to measure the urinary recovery rates of inulin. The results were comparable to those obtained in rabbits (Table 7). The test was carried out by the Lunt method.
TABLE 7______________________________________ Level of addition of Dose of Enamine derivative % UrinarySubject inulin (I) recovery______________________________________Rabbit mg/body Compound 3 10% 8%Dog " " 5% 20%Human " " 1% 11%Human " Compound 1 2% 12%______________________________________
Production Example 3 [Enamine derivative (I)]
The material amino acids or sodium salts thereof indicated in Table 8 were dissolved or suspended in the solvents also indicated in the same table. The resultant solutions or suspensions were each stirred at room temperature or an elevated temperature. Then, an equimolar amount of ethylacetoacetate was added dropwise and the mixture was stirred for 2 to 10 hours. The solvent was distilled off under reduced pressure and the solid residue was washed or recrystallized. By the above procedure were obtainedthe contemplated products. The physical properties of these products are shown in Tables 9, 10 and 11.
TABLE 8______________________________________Material amino acid or Yieldsodium salt thereof Solvent Temperature (%)______________________________________18 DL-Alanine (Na) methanol room 64.519 L-Alanine (Na) " " 60.020 L-Isoleucine (Na) " " 75.021 L-Leucine (Na) " " 60.022 L-Threonine (Na) " " 70.023 L-Methionine (Na) " " 72.024 DL-Phenylglycine (Na) " " 88.025 L-Phenylalanine (Na) " " 97.026 L-Valine (Na) " 60-80.degree. C. 98.427 L-Tryptophan (Na) " " 96.828 L-Histidine (Na) ethanol room 97.829 L-Arginine " 60-80.degree. C. 80.530 11-Aminoundecanoic " " 84.3 acid (Na)31 L-Serine (Na) " room 80.5______________________________________(Na): Sodium salt
The following contemplated compounds were obtained.
18. N-(1-Methyl-2-ethoxycarbonylvinyl)-DL-alanine sodium salt (Compound 18)
19. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-alanine sodium salt (Compound 19)
20. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-isoleucine sodium salt (Compound 20)
21. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-leucine sodium salt (Compound 21)
22. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-threonine sodium salt (Compound 22)
23. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-methionine sodium salt (Compound 23)
24. N-(1-Methyl-2-ethoxycarbonylvinyl)-DL-phenylglycine sodium salt (Compound 24)
25. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-phenylalanine sodium salt (Compound 25)
26. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-valine sodium salt (Compound 26)
27. .alpha.-N-(1-Methyl-2-ethoxycarbonylvinyl)-L-tryptophan sodium salt (Compound 27)
28. .alpha.-N-(1-Methyl-2-ethoxycarbonylvinyl)-L-histidine sodium salt (Compound 28)
29. .alpha.-N-(1-Methyl-2-ethoxycarbonylvinyl)-L-arginine (Compound 29)
30. N-(1-Methyl-2-ethoxycarbonylvinyl)-11-aminoundecanoic acid sodium salt (Compound 30)
31. N-(1-Methyl-2-ethoxycarbonylvinyl)-L-serine sodium salt (Compound 31)
TABLE 9______________________________________Compound Specific rotationNo. mp. (.degree.C.) values [.alpha.].sub.D______________________________________18 71-75 (dec) 0 (H.sub.2 O 23.degree.)19 157-159 (dec) +187.0 (H.sub.2 O 23.degree.)20 166-167 (dec) +152.0 (H.sub.2 O 26.degree.)21 147-149 +115.0 (H.sub.2 O 23.degree.)22 183-187 (dec) +142.0 (H.sub.2 O 23.degree.)23 108-110 +9.0 (H.sub.2 O 23.degree.)24 199-201 (dec) 0 (H.sub.2 O 23.degree.)25 96.0-97.5 -262.5 (Absolute alcohol 23.degree.)26 60.0-65.0 +125.0 (Methanol 29.degree.)27 118-120 (dec) -260.0 (Methanol 29.degree.)28 110-112 (dec) -135.0 (Absolute alcohol 24.degree.)29 120 +52.0 (Methanol 29.degree.)30 180-190 (dec) --31 193-194 (dec) +110.0 (H.sub.2 O 24.degree.)______________________________________
TABLE 10______________________________________CompoundNo. UV.sub.(m.mu.).sup.(.lambda. max, ethanol) IR.sub.(cm.spsb.-1.sub.).sup.(Nujol)______________________________________18 287 1630, 157019 387 1630, 157020 290 1640, 1620, 158021 288 1640, 156022 288 1650, 1610, 157023 288 1630, 159024 286 1640, 156025 288 1630, 1610, 158026 288 1640, 159027 284, 290 1630, 158028 287 1640, 159029 286 1630, 157030 284 1640, 156031 287 1660, 1650, 1620, 1570______________________________________
TABLE 11______________________________________CompoundNo. H.sub.A H.sub.B H.sub.C______________________________________18 9.00 (d,9,1.sup.H) 1.85 (s,3.sup.H) 4.20 (a,1.sup.H)19 8.96 (d,8,1.sup.H) 1.85 (s,3.sup.H) 4.20 (s,1.sup.H)20 8.90 (d,9,1.sup.H) 1.80 (s,3.sup.H) 4.20 (s,1.sup.H)21 8.70 (d,9,1.sup.H) 1.80 (s,3.sup.H) 4.15 (s,1.sup.H)22 8.87 (d,8,1.sup.H) 1.86 (s,3.sup.H) 4.22 (s,1.sup.H)23 8.85 (d,8,1.sup.H) 1.85 (s,3.sup.H) 4.20 (s,1.sup.H)24 9.50 (d,6,1.sup.H) 1.60 (s,3.sup.H) 4.22 (s,1.sup.H)25 8.85 (d,9,1.sup.H) 1.46 (s,3.sup.H) 4.10 (s,1.sup.H)26 8.95 (d,9,1.sup.H) 1.85 (s,3.sup.H) 4.25 (s,1.sup.H)27 8.95 (d,9,1.sup.H) 1.45 (s,3.sup.H) 4.05 (s,1.sup.H)28 8.85 (d,9,1.sup.H) 1.60 (s,3.sup.H) 4.12 (s,1.sup.H)29 8.85 (d,9,1.sup.H) 1.85 (s,3.sup.H) 4.25 (s,1.sup.H)30 -- (disappear) 1.75 (s,3.sup.H) 4.35 (s,1.sup.H)31 9.00 (d,7,1.sup.H) 1.80 (s,3.sup. H) 4.25 (s,1.sup.H) (ppm)______________________________________(Note)1. The solvent was DMSOd.sub.6, except that D.sub.2 O was used for Compoun30. ##STR13##
EXAMPLE 7
______________________________________(Recipes 21-34: rectal suppositories)______________________________________Amorphous insulin 6 I.U.Enamine derivative (I) As indicated in Table 12Witepsol H-15.RTM. To make a total of 0.5g______________________________________
The above components were used to prepare rectal suppositories in accordance with established pharmaceutical practice. The suppositories were administered to rabbits and dogs by rectal route.
Table 12 shows the glucose responses (%) obtained with the above suppositories. The test was conducted by the glucose oxidase method.
TABLE 12______________________________________ Time course (min.) Return Blood to Kind glucose pre-Level of addition of response medi-of enamine deriva- ani- (6 I.U.) On- Lowest cationtive (I) mal (%) set level level______________________________________Compound 18, 1% R 45-55 30 45-60 180Compound 19, 1% R 45-55 30 45-60 180Compound 20, 2% D 25-35 60-90 120 180-240Compound 21, 1% D 35-45 30 60-90 180Compound 22, 5% R 40-50 30 45-75 180Compound 23, 10% R 30 30 60-90 180Compound 24, 1% R 50-65 15 30-60 180Compound 25, 1% R 50-65 30 45-90 180Compound 26, 10% R 30 30 60-90 180Compound 27, 10% R 30 30 60-90 180Compound 28, 10% R 30 30 60-90 180Compound 29, 5% R 40-50 15 60-90 210Compound 30, 10% R 30 30 60-90 180Compound 31, 10% R 30 30 60-90 180______________________________________(Note)R: rabbitD: dog
Production Example 4 [Enamine derivatives (I)]
The amino acids and starting compounds (V) indicated in Table 13 were reacted under the conditions also indicated in the same table. The reaction was carried out in homogenous solution or suspension for 2 to 10 hours.
The reaction was conducted at room temperature or an elevated temperature, with constant stirring. The solvent was then distilled off and the solid residue was recrystallized to obtain the contemplated enamine derivative.
TABLE 13__________________________________________________________________________ Material Material compound (V)* Tempera-No. amino acid (R.sub.5 in formula V) Solvent ture__________________________________________________________________________32 Glycine (Na) ##STR14## Ethanol Room33 DL-Alanine (Na) ##STR15## Ethanol Reflux34 L-phenylalanine (Na) ##STR16## Ethanol Room35 L-arginine ##STR17## H.sub.2 O Room36 L-phenylalanine (Na) ##STR18## Methanol Room37 L-phenylalanine (Na) ##STR19## Ethanol Room38 Glycine (Na) ##STR20## Ethanol Room__________________________________________________________________________(Note)*R.sub.3 = CH.sub.3, R.sub.4 = H(Na): Sodium salt
The chemical names of enamine derivatives (I) thus obtained are as follows.
32. N-[1-Methyl-2-(1-ethoxycarbonyl-ethoxycarbonyl)vinyl]glycine sodium salt (Compound 32)
33. N-[1-Methyl-2-(1-ethoxycarbonyl-ethoxycarbonyl)vinyl]-DL-alanine sodiumsalt (Compound 33)
34. N-[1-Methyl-2-(1-ethoxycarbonyl-ethoxycarbonyl)vinyl]-L-phenylalanine sodium salt (Compound 34)
35. .alpha.-N-(1-Methyl-2-(1-carboxy-ethoxycarbonyl)vinyl]-L-arginine (Compound 35)
36. N-[1-Methyl-2-(2-hydroxyethylcarbamoyl)vinyl]-L-phenylalanine sodium salt (Compound 36)
37. N-(1-Methyl-2-ethoxycarbonylmethylcarbamoyl-vinyl)-L-phenylalanine sodium salt (Compound 37)
38. N-[1-Methyl-2-(2,3-dihydroxypropoxycarbonyl)vinyl]glycine sodium salt (Compound 389
The melting points, UV spectra, IR spectra and specific rotation values of these product compounds are shown in Table 14.
TABLE 14______________________________________ UV.sub.(.lambda.max) SpecificCom- m.mu. rotationpound (95% IR value [.alpha.].sub.DNo. mp. (.degree.C.) ethanol) (cm.sup.-1) (Ethanol 20.degree. C.)______________________________________ 172532 -- 288 1640 -- 1580 173033 -- 288 1640 -- 1580 173034 -- 292 1640 -- 1580 164035 -- 286.5 1570 +34.degree. 162036 129-135 287 1580 -183.7.degree. 161037 101-104 288 1580 -218.9.degree.______________________________________
The NMR spectra of Compound 32 through 37 are as follows. ##STR21##
TABLE 15______________________________________Com-pound Sol-No. R' H.sup.A H.sup.B H.sup.C H.sup.D vent______________________________________32 C.sub.2 H.sub.5 8.72 1.85 4.30 4.82 DMSO (t,J=5,1H) (s,3.sup.H) (s,1.sup.H) (q,J=7,1.sup.H)33 C.sub.2 H.sub.5 8.90 1.87 4.23 4.81 " (d,J=7,1H) (s,3.sup.H) (s,1.sup.H) (q,J=7,1.sup.H)34 C.sub.2 H.sub.5 8.80 1.50 4.15 4.82 " (d,J=8,1H) (s,3.sup.H) (s,1.sup.H) (q,J=7,1.sup.H)35 H 1.90 4.82 (s,3.sup.H) (q,J=7,1.sup.H) D.sub.2 O______________________________________
______________________________________ ##STR22## (Compound 36)H.sub.A 9.15 ppm (d, J= 9 Hz, 1H)H.sub.B 1.40 ppm (s, 3H)H.sub.C 4.10 ppm (s, 1H) ##STR23## 7.17 ppm (s, 5H)______________________________________ ##STR24## (Compound 37)H.sub.A 9.50 ppm (d, J= 9 Hz, 1H)H.sub.B 1.08 ppm (s, 3H)H.sub.C 4.06 ppm (s, 1H)H.sub.D 1.23 ppm (t, 3H, 7 Hz)H.sub.E 4.10 ppm (q, 2H, 7 Hz) ##STR25## 7.10 ppm (s, 5H)______________________________________
EXAMPLE 8
______________________________________(Recipe 35-41: rectal suppositories)______________________________________Amorphous insulin 6 I.U.Enamine derivative (I) 0.025gWetspsol H-15.RTM. 0.475g______________________________________
The above components were used to prepare rectal suppositories in accordance with established pharmaceutical practice. These suppositories were rectally administered to rabbits (R, 2.5-3kg) and dogs (D, 12-13kg). The glucose responses (%) and time courses (min.) are shown in Table 16.
TABLE 16______________________________________ Time course (min.) Return Blood to Kind glucose pre-Level of addition of response medi-of enamine deri- ani- (6 I.U.) On- Lowest cationvative (I) mal (%) set level level______________________________________Compound 32, 5% R 45-55 15 45-60 150Compound 32, 5% D 30-40 30 30-60 120Compound 33, 5% R 45-55 15 45-60 150Compound 34, 5% R 45-55 15 45-60 150Compound 35, 5% R 20-30 15 30-60 120Compound 36, 5% R 20-30 15 30-60 120compound 37, 5% R 20-30 30 30-60 120Compound 38, 5% R 45-55 15 45-60 150______________________________________R: RabbitD: Dog
EXAMPLE 9
Suppositories were prepared in accordane with established pharmaceutical practice, using the recipes shown in Table 17. These suppositories were rectally administered to dogs and the absorbabilities of the active components were investigated by measuring the blood concentrations. The results are shown in Table 17.
The blood concentrations were measured in conformity with the biological assay method in the Antibiotic Standard of Japan. The cup method was employed with Sarcina lutea for penicillins and Bacillus subtilis for cephalosporins.
TABLE 17__________________________________________________________________________RecipeEnamine Concentration in blood (.mu.g/ml)derivative Body weight 15 30 60 120(I) Antibiotics base (Dog) (kg) min. min. min. min.__________________________________________________________________________Compound 1 AB-PC-Na SB-W 13.5 1.23 0.87 0.61 0.3260mg 75mg 1065mg(50%)Compound 10 CET-Na Witepsol 12.0 15.30 11.73 6.75 2.20150mg 500mg H-15(10%) 850mgCompound 20 CET-Na Witepsol 9.8 9.00 6.23 2.91 1.00150mg 500mg H-15(10%) 850mgCompound 22 CET-Na Witepsol 9.5 0.75 0.57 0.31 --60mg 75mg H-15(5%) 1065mgCompound 27 AB-PC-Na SB-W 11.4 1.6 1.5 0.74 0.40120mg 75mg 1005mg(10%)Compound 23 AB-PC-Na Witepsol 13.0 0.52 0.34 0.18 0.0860mg 75mg H-15(5%) 1065mgCompound 28 AB-PC-Na SB-W 10.5 0.56 0.44 0.14 --60mg 75mg 1065mg(5%)Compound 29 AB-PC-Na SB-W 11.5 0.50 0.53 0.61 0.41120mg 75mg 1005mg(10%)Compound 16 AB-PC-Na SB-W 13.0 0.69 0.48 0.52 0.29120mg 75mg 1005mg(10%)Compound 31 AB-PC-Na Witepsol 11.3 0.70 0.38 0.24 0.0960mg 75mg H-15(5%) 1065mgCompound 32 CET-Na SB-W 11.5 1.50 1.12 0.73 0.4060mg 75mg 1065mg(5%)Compound 34 CET-Na Witepsol 9.5 1.25 0.83 0.61 0.3960mg 75mg H-15(5%) 1065mgCompound 36 AB-PC-Na Witepsol 12.0 1.45 1.53 0.80 0.40120mg 75mg H-15(10%) 1005mgCompound 37 CET-Na Witepsol 12.5 1.50 1.28 0.87 0.44120mg 75mg H-15(10%) 1005mg__________________________________________________________________________Na: Sodium salt

Number	Date	Country	Kind
53-104130	Aug 1978	JPX
54-97931	Jul 1979	JPX

Adjuvant for promoting absorption of therapeutically active substances through the digestive tract

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