Claims
- 1. A purified polynucleotide comprising a nucleotide sequence encoding a polypeptide that comprises a fragment of a mammalian Asp2 protein, wherein said polypeptide lacks the Asp2 transmembrane domain of said Asp2 protein, and wherein the polypeptide and the fragment retain β-secretase activity of said Asp2 protein.
- 2. A polynulcleotide of claim 2 wherein the polypeptide comprises a fragment of human Asp2 protein.
- 3. A polynucleotide of claim 2 wherein the polypeptide comprises a fragment of Asp2(a) having the amino acid sequence set forth as SEQ ID NO: 4, and wherein the polypeptide lacks the transmembrane domain amino acids 455-477 of SEQ ID NO: 4.
- 4. A polynucleotide of claim 3, wherein the polypeptide further lacks cytoplasmic domain amino acids 478-501 of SEQ ID NO: 4.
- 5. A purified polynucleotide of claim 4, wherein said polypeptide further lacks amino acids 420-454 of SEQ ID NO: 4.
- 6. A polynucleotide of claim 3, wherein the polypeptide comprises an amino acid sequence:
that includes amino acids 58-419 of SEQ ID NO: 4, and that lacks amino acids 22-57 of SEQ ID NO: 4.
- 7. A polynucleotide of claim 3, wherein the polypeptide comprises an amino acid sequence:
that includes amino acids 46-419 of SEQ ID NO: 4, and that lacks amino acids 22-45 of SEQ ID NO: 4.
- 8. A polynucleotide of claim 3, wherein the polypeptide comprises an amino acid sequence that includes amino acids 22-454 of SEQ ID NO: 4.
- 9. A polynucleotide of claim 2, wherein the polypeptide comprises a fragment of human Asp2(b) having the amino acid set forth in SEQ ID NO: 6, and wherein the polypeptide lacks transmembrane domain amino acids 430-452 of SEQ ID NO: 6.
- 10. A polynucleotide of claim 9, wherein the polypeptide lacks cytoplasmic domain amino acids 453-476 of SEQ ID NO: 6.
- 11. A polynucleotide of claim 10, wherein the polypeptide further lacks amino acids 395-429 of SEQ ID NO: 6.
- 12. A polynucleotide of claim 9, wherein the polypeptide comprises an amino acid sequence:
that includes amino acids 58-394 of SEQ ID NO: 6, and that lacks amino acids 22 to 57 of SEQ ID NO: 6.
- 13. A polynucleotide of claim 10, wherein the polypeptide comprises an amino acid sequence:
that includes amino acids 46-394 of SEQ ID NO: 6, and that lacks amino acids 22-45 of SEQ ID NO: 6.
- 14. A polynucleotide of claim 9, wherein the polypeptide comprises an amino acid sequence that includes amino acids 22 to 429 of SEQ ID NO: 6.
- 15. A vector comprising a polynucleotide of claim 1.
- 16. A host cell transformed or transfected with a vector of claim 15.
- 17. A host cell transformed or transfected with a polynucleotide of claim 1.
- 18. A polynucleotide comprising a nucleotide sequence that hybridizes under stringent conditions to a nucleic acid comprising the sequence set forth in SEQ ID NO: 4 or SEQ ID NO: 6, wherein the nucleotide sequence encodes a polypeptide having β-secretase biological activity.
- 19. A vector comprising a polynucleotide of claim 18.
- 20. A host cell transformed or transfected with a polynucleotide of claim 19.
- 21. A vector comprising a polynucleotide of claim 3.
- 22. A host cell transformed or transfected with a vector of claim 21.
- 23. A vector comprising a polynucleotide of claim 4.
- 24. A host cell transformed or transfected with a vector of claim 23.
- 25. A vector comprising a polynucleotide of claim 5.
- 26. A host cell transformed or transfected with a vector of claim 25.
- 27. A vector comprising a polynucleotide of claim 6.
- 28. A host cell transformed or transfected with a vector of claim 27.
- 29. A vector comprising a polynucleotide of claim 7.
- 30. A host cell transformed or transfected with a vector of claim 29.
- 31. A vector comprising a polynucleotide of claim 8.
- 32. A host cell transformed or transfected with a vector of claim 31.
- 33. A vector comprising a polynucleotide of claim 9.
- 34. A host cell transformed or transfected with a vector of claim 33.
- 35. A vector comprising a polynucleotide of claim 10.
- 36. A host cell transformned or transfected with a vector of claim 35.
- 37. A vector comprising a polynucleotide of claim 11.
- 38. A host cell transformed or transfected with a vector of claim 37.
- 39. A vector comprising a polynucleotide of claim 12.
- 40. A host cell transformed or transfected with a vector of claim 39.
- 41. A vector comprising a polynucleotide of claim 13.
- 42. A host cell transformed or transfected with a vector of claim 41.
- 43. A vector comprising a polynucleotide of claim 14.
- 44. A host cell transformed or transfected with a vector of claim 43.
Parent Case Info
[0001] The present application is a continuation of U.S. patent application Ser. No. 09/416,901, filed Oct. 13, 1999, which claims priority benefit of U.S. Provisional Patent Application No. 60/155,493, filed Sep. 23, 1999; and which also claims priority benefit as a continuation-in-part of U.S. patent application Ser. No. 09/404,133 and PCT/US99/20881, both filed Sep. 23, 1999, both of which in turn claim priority benefit of U.S. Provisional Patent Application No. 60/101,594, filed Sep. 24, 1998. All of these priority applications are hereby incorporated by reference in their entirety.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60155493 |
Sep 1999 |
US |
|
60101594 |
Sep 1998 |
US |
Continuations (3)
|
Number |
Date |
Country |
Parent |
09416901 |
Oct 1999 |
US |
Child |
09795847 |
Feb 2001 |
US |
Parent |
09404133 |
Sep 1999 |
US |
Child |
09795847 |
Feb 2001 |
US |
Parent |
PCT/US99/20881 |
Sep 1999 |
US |
Child |
09795847 |
Feb 2001 |
US |