Claims
- 1. The method of making water-soluble amidated immune globulins which comprises reacting immune globulins with a molar excess of a water-soluble primary monoamine and a water-soluble carbodiimide or a salt thereof in a slightly acidic to neutral aqueous solution.
- 2. A method as in claim 1 wherein the molar ratio of amine to immune globulines is at least 50:1 and the ratio of carbodiimide to immune globulines is at least 1:1.
- 3. A method as in claim 1 wherein the molar ratio of carbodiimide to immune globulines is from 5:1 to 20:1.
- 4. A method as in claim 1 wherein said amine is an aliphatic monoamine having 1 to 10 carbon atoms.
- 5. A method as in claim 4 wherein said aliphatic monoamine is hydroxy-substituted.
- 6. A method as in claim 4 wherein said aliphatic monomine is acetal-substituted.
- 7. A method as in claim 1 wherein said amine is ethanol amine, trishydroxymethylaminomethane, or glucosamine.
- 8. A method as in claim 1 wherein said carbodiimide is 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride or 1-cyclohexyl-3-(2-morpholino-ethyl)carbodiimide-metho-p-toluene sulfonate.
- 9. A method as in claim 1 wherein the reaction is carried out at a pH-value of 3 to 7.
- 10. A method as in claim 1 wherein the reaction is carried out at a temperature from 5.degree. to 50.degree. C.
- 11. A method as in claim 1 wherein said immune globulins are human immune globulins.
- 12. A method as in claim 1 wherein said immune globulins comprise at least one disulfide bond converted into two sulfhydril groups with the aid of a reducing agent.
- 13. A method as in claim 12 wherein the conversion of the disulfide bond into sulfhydril groups is carried out with a reducing agent at a slightly alkaline pH-value.
- 14. A method as in claim 13 wherein the concentration of the reducing agent is 0.01 mol/1 and the molar ratio of protein to reducing agent is 2.5:1 to 50:1.
- 15. A method as in claim 12 wherein said reducing agent is dithioerythritol or dithiothreitol.
- 16. Water-soluble amidated immune globulins prepared by the method of claim 1.
- 17. Water-soluble amidated immune globulins prepared by the method of claim 12.
Priority Claims (1)
Number |
Date |
Country |
Kind |
2442655 |
Sep 1974 |
DEX |
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Parent Case Info
This is a continuation of application Ser. No. 610,458, filed Sept. 4, 1975, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
3857931 |
Hager |
Dec 1974 |
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Continuations (1)
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Number |
Date |
Country |
Parent |
610458 |
Sep 1975 |
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