Claims
- 1. A kit comprising (a) a single stranded DNA oligomer bonded at its 3' end to a single stranded polynucleotide binding sequence wherein said binding sequence is complementary to a polynucleotide target sequence comprising 12 to 1000 nucleotides, wherein said oligomer consists of about 3 to 100 oligonucleotide units each consisting of an identical oligonucleotide template sequence having about 8 to 100 nucleotides and at least one restriction site when said template sequence is hybridized to a complementary sequence, wherein said oligomer is consists of only three different nucleotides, said nucleotides being selected from the group consisting of dATP, dTTP, dGTP and dCTP and derivatives thereof, (b) deoxynucleoside triphosphates, (c) DNA-dependent DNA polymerase, (d) restriction endonuclease capable of cleaving said restriction site.
- 2. The kit of claim 1 which further comprises in combination a single stranded DNA primer comprising a nucleic acid sequence capable of hybridizing with RNA and containing a restriction site, said primer being terminated at its 3' end by a group incapable of reacting in a chain extension by said DNA-dependent DNA polymerase.
- 3. The kit of claim 2 which further comprises a restriction enzyme capable of cleaving said primer at said restriction site when said primer is hybridized with said RNA sequence.
Parent Case Info
This is a divisional of pending application Ser. No. 07/076,807, filed Jul. 23, 1987, now U.S. Pat. No. 4,994,368.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4656134 |
Ringold |
Apr 1987 |
|
4948731 |
Gehrke et al. |
Aug 1990 |
|
Divisions (1)
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Number |
Date |
Country |
Parent |
76807 |
Jul 1987 |
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