Claims
- 1. A method for screening for a compound which reduces the formation of .beta.-amyloid protein, comprising the steps of:
- (a) measuring the amount of marker in a suitable medium containing transfected cells stably or transiently expressing a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform,
- (b) treating said cells with the compound, and
- (c) testing the medium for an increase in the amount of the marker.
- 2. The method of claim 1, wherein the compound is a protein kinase C activator.
- 3. The method of claim 1, which further comprises contacting the treated cells with an antibody directed to a portion of a .beta.-amyloid protein domain sequence under suitable conditions to favor the formation of an antibody-antigen complex, and detecting the presence of any complex so formed.
- 4. A method for screening for a compound which reduces the formation of .beta.-amyloid protein, comprising the steps of:
- (a) measuring the amount of marker in a suitable medium containing transfected cells stably or transiently expressing a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform wherein the amino acid residues from position 11 to position 28 are deleted from the portion of the nucleic acid sequence encoding the .beta.-amyloid protein domain and the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform,
- (b) treating said cells with the compound, and
- (c) testing the medium for an increase in the amount of the marker.
- 5. The method of claim 4, which further comprises contacting the treated cells with an antibody directed to a portion of a .beta.-amyloid protein domain sequence under suitable conditions to favor the formation of antibody-antigen complex, and detecting the presence of any complex so formed.
- 6. A method for screening for a compound which reduces the formation of .beta.-amyloid protein, comprising the steps of:
- (a) measuring the amount of marker in a suitable medium containing transfected cells stably or transiently expressing a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform and in which an alanine substitution at a phosphorylation site within the cytoplasmic domain of an amyloid precursor protein is encoded, wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform,
- (b) treating said cells with the compound, and
- (c) testing the medium for an increase in the amount of the marker.
- 7. The method of claim 6, which further comprises contacting the treated cells with an antibody directed to a portion of a .beta.-amyloid protein domain sequence under suitable conditions to favor the formation of an antibody-antigen complex, and detecting the presence of any complex so formed.
RELATED U.S. APPLICATION DATA
This is a divisional of application Ser. No. 08/123,659 filed on Sep. 20, 1993, which is a continuation in part of application Ser. No. 07/877,675 filed on May 1, 1992, now abandoned.
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Sep 1994 |
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Divisions (1)
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Number |
Date |
Country |
Parent |
123659 |
Sep 1993 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
877675 |
May 1992 |
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