Claims
- 1. A purified and isolated polypeptide encoded by the nucleic acid sequence of a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform, wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform.
- 2. A purified and isolated polypeptide encoded by the nucleic acid sequence of a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform, wherein the amino acid residues from position 11 to position 28 are deleted from the portion of the nucleic acid sequence encoding the .beta.-amyloid protein domain and the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform.
- 3. A purified and isolated polypeptide encoded by the nucleic acid sequence of a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform in which an alanine substitution at a phosphorylation site within the cytoplasmic domain of an amyloid precursor protein is encoded, wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform.
RELATED U.S. APPLICATION DATA
This is a divisional of application Ser. No. 08/123,659 filed on Sep. 20, 1993, which is a continuation-in-part of application Ser. No. 07/877,675 filed on May 1, 1992, now abandoned.
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3318793 |
Oct 1993 |
AUX |
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Sep 1991 |
EPX |
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Non-Patent Literature Citations (2)
Entry |
Sahasrabudhe, S.R. et al; J. Biol. Chem. 267:25602-25608 (1992). |
Vitek, M.P. et al., Soc. Neurosci. Abstr. 17(1-2):1443 (1991). |
Divisions (1)
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Number |
Date |
Country |
Parent |
123659 |
Sep 1993 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
877675 |
May 1992 |
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