Claims
- 1. An immunogenic composition, comprising an immunoeffective amount of an isolated and purified analog of Haemophilus influenzae Hin 47 protein wherein multiple amino acids of the natural Hin47 protein contributing to protease activity have been deleted or replaced by different amino acids to provide a reduced protease activity which is less than 10% of that of the natural Hin47 protein and having substantially the same immunogenic properties as natural Hin47 protein.
- 2. The immunogenic composition of claim 1 formulated as a vaccine for in vivo administration to a host to confer protection against diseases caused by a bacterial pathogen that produces Hin47 protein or a protein capable of inducing antibodies in the host specifically reactive with Hin47 protein.
- 3. The immunogenic composition of claim 2 wherein the bacterial pathogen is a Haemophilus species.
- 4. The immunogenic composition of claim 3 wherein the Haemophilus species is Haemophilus influenzae.
- 5. The immunogenic composition of claim 2 further comprising at least one other immunogenic or immunostimulating material.
- 6. An immunogenic composition, comprising an immunoeffective amount of an isolated and purified analog of Haemophilus influenzae Hin 47 protein wherein multiple amino acids of the natural Hin47 protein contributing to protease activity have been deleted or replaced by different amino acids to provide a reduced protease activity which is less than 10% of that of the natural Hin47 protein and having substantially the same immunogenic properties as natural Hin47 protein, wherein at least one amino acid of said identified multiple amino acids is selected from amino acids 95 to 201 of natural Hin47 protein.
- 7. The composition of claim 6 wherein said at least one amino acid is Serine-197.
- 8. The composition of claim 6 wherein Serine-197 is replaced by alanine, cysteine or threonine.
- 9. An immunogenic composition, comprising an immunoeffective amount of an isolated and purified analog of Haemolhilus influenzae Hin 47 protein wherein multiple amino acids of the natural Hin47 protein contributing to protease activity have been deleted or replaced by different amino acids to provide a reduced protease activity which is less than 10% of that of the natural Hin47 protein and having substantially the same immunogenic properties as natural Hin47 protein, wherein at least one amino acid of said identified multiple amino acids is Histidine-91 or Asp-121 of natural Hin47 protein.
- 10. The composition of claim 9 wherein Histidine-91 is replaced by alanine, lysine or arginine.
- 11. The composition of claim 9 wherein Asp-121 is replaced by alanine.
- 12. An immunogenic composition, comprising an immunoeffective amount of an isolated and purified analog of Haemophilus influenzae Hin 47 protein wherein multiple amino acids of the natural Hin47 protein contributing to protease activity have been deleted or replaced by different amino acids to provide a reduced protease activity which is less than 10% of that of the natural Hin47 protein and having substantially the same immunogenic properties as natural Hin47 protein, wherein the multiple amino acids are His-91 and Ser-197 and are deleted or replaced by alanine.
REFERENCE TO RELATED APPLICATION
This is a divisional of Ser. No. 08/487,167 filing date Jun. 7, 1995 now U.S. Pat No. 5,869,302 which is a continuation-in-part of Ser. No 08/296,149 filed Aug. 26, 1994, now abandoned, which is a continuation-in-part of Ser. No. 08/278,091 filed Jul. 21, 1994, now U.S. Pat. No. 5,506,139.
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Divisions (1)
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Number |
Date |
Country |
Parent |
487167 |
Jun 1995 |
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Continuation in Parts (2)
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Number |
Date |
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Parent |
296149 |
Aug 1994 |
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Parent |
278091 |
Jul 1994 |
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