Antagonists of LHRH

Information

  • Patent Grant
  • 5656727
  • Patent Number
    5,656,727
  • Date Filed
    Friday, April 28, 1995
    29 years ago
  • Date Issued
    Tuesday, August 12, 1997
    27 years ago
Abstract
LHRH analogs and congeners with high water solubility have been synthesized. These new analogs had 0%-100% antiovulatory activity at a 0.5 .mu.g dosage and 0%-80% at 0.25 .mu.g. The ED.sub.50 for histamine release was 30.5 .mu.g/ml->300 .mu.g/ml.
Description

BACKGROUND OF THE INVENTION
Many potent analogs of LHRH (the luteinizing hormone releasing hormone, pGlu.sup.1, His.sup.2, Trp.sup.3, Ser.sup.4, Tyr.sup.5, Gly.sup.6, Leu.sup.7, Arg.sup.8, Pro.sup.9, Gly.sup.10 - NH.sub.2) have structural features which cause release of histamine from mast cells (Karten et al., 1986). These features include a group of hydrophobic amino acids at the N-terminal and strongly basic residues in positions 6 and 8, notably D-Arg.sup.6, Arg.sup.8. A prime example of this class of analogs is (N-Ac-D-2-Na1.sup.1, D-4F-Phe.sup.2 D-Trp.sup.3, D-Arg.sup.6)- LHRH (Schmidt et al.). Some analogs bind to rat peritoneal mast cells and membrane preparations and that the binding was related to the release of histamine (Sundaram et al.). For a recent review of LHRH analogs, see Janecka, et al. (1994).
Abbreviations for the unnatural amino acids mentioned herein are included in the following list:
______________________________________AABLys N.sup..epsilon. -(4-acetylaminobenzoyl)lysineAALys N.sup..epsilon. -anisinoyl-lysineAAPhe 3-(4-acetylaminophenyl)lysineAbu 2-aminobutyric acidACLys N.sup..epsilon. -(6-aminocaproyl)lysineACyh 1-aminocyclohexanecarboxylic acidACyp 1-aminocyclopentanecarboxylic acidAile alloisoleucineAla alanineAnGlu 4-(4-methoxy-phenylcarbamoyl)-2-aminobutyric acid2ANic 2-aminonicotinic acid6ANic 6-aminonicotinic acidAPic 6-aminopicolinic acidAPh 4-aminobenzoic acidAPhe 4-aminophenylalanineAPz 3-amino-2-pyrazinecarboxylic acidAze azetidine-2-carboxylic acidBim 5-benzimidazolecarboxylic acidBzLys N.sup..epsilon. -benzoyllysineCit citrullineCl.sub.2 Phe 3-(3,4-dichlorophenyl)alanineCpa 3-(4-chlorophenyl)alaninecPzACAla cis-3-(4- pyrazinylcarbonylaminocyclohexyl)alaninecPmACAla cis-3-(4-(4- pyrimidylcarbonyl)aminocyclohexyl)alanineDbf 3-(2-dibenzofuranyl)alanineDMGLys N.sup..epsilon. -(N,N-dimethylglycyl)lysineDpo N.sup..epsilon. -(4,6-dimethyl-1-pyrimidyl)-ornithineF.sub.2 Ala 3,3-difluoroalaninehNal 4-(2-naphthyl)-2-aminobutyric acidHOBLys N.sup..epsilon. -=(4-hydroxybenzoyl)lysinehpClPhe 4-(4-chlorophenyl)-2-amino-butyric acidHse homoserine (2-amino-4-hydroxybutanoic acid)ICapLys N.sup..epsilon. -(6-isopropylaminocaproyl)lysineILys N.sup..epsilon. -isopropyllysineInd indoline-2-carboxylic acidINicLys N.sup..epsilon. -isonicotincyllysineIOrn N.sup..delta. -isopropylornithineMe.sub.3 Arg N.sup.G,N.sup.G,N.sup.G -trimethylarginineMe.sub.2 Lys N.sup..epsilon., N.sup..epsilon. -dimethyllysineMNal 3-((6-methyl)2-naphtyl)alanineMNicLys N.sup..epsilon. -(6-methylpicolinoyl)lysineMOB 4-methoxybenzoylMpClPhe N-methyl-3-(4-chlorophenyl)lysineMPZGlu glutamic acid, y-4-methylpiperazineNAcDNal N-acetyl-3-12-naphthyl)alanineNacDQal N-acetyl-3-(3-quinolyl)alanineNal 3-(2-naphthyl)alanineNap 2-naphthoic acidNapDThr N-naphthoyl-D-threonineNicLys N.sup..epsilon. -nicotinoyllysineNO.sub.2 B 4-nitrobenzoylNO.sub.2 Phe 3-(4-nitrophenyl)alanineoClPhe 3-(2-chlorophenyl)alanineOpt O-phenyl-tyrosineOrn ornithinePal 3-(3-pyridyl)alanine21Pal 3-(2-pyridyl)alanine2PALys N.sup..epsilon. -(3-pyridylacetyl)lysinepCapLys N.sup..epsilon. -(5-picolinoylaminocaproyl)lysinepClPhe 3-(4-chlorophenyl)alaninepFPhe 3-(4-fluorophenyl)-alaninePic picolinic acidPicLys N.sup..epsilon. -picolinoyllysinePicSar N-picolinoylsarcosinylPip piperidine-2-carboxylic acidPmcLys N.sup..epsilon. --(4-pyrimidylcarbonyl)lysinePtf 3-(4-trifluoromethyl) phenylalaninePz pyrazinecarboxylic acidPzAla 3-pyrazinylalaninePzAPhe 3-(4-pyrazinylcarbonylaminophenyl)alanineQal 3-(3-quinolyl)alanineQnd-Lys N.sup..epsilon. -quinaldoyllysineQui 3-quinolinecarboxylic acidQux 2-quinoxalinecarboxylic acidTic 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acidTinGly 2-thienylglycinetNACAla trans-3-(4-nicotinoylaminocyclohexyl)-alaninetPACAla trans-3-(4-picolinoylaminocyclohexyl)alanine______________________________________
SUMMARY OF THE INVENTION
LHRH analogs having the following formulas have been prepared and tested.
(NAcDQal.sup.1, DPtf.sup.2, DPal.sup.3, cisPzACAla.sup.5, DPicLys.sup.6, DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, cisPzACAla.sup.5, DNicLys.sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, Thr.sup.4, PicLys.sup.5, DPicLys.sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, DPicLys.sup.6, Thr.sup.7, ILys.sup.8, DAla.sup.10)LHRH
(NAcDThr.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, DPicLys.sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, NicLys.sup.5, DNicLys.sup.6, Thr.sup.7, ILys.sup.8,DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, Thr.sup.4 NicLys.sup.5, DNicLys.sup.6, Thr.sup.7, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, D(PicSar) ILys.sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, D(PicSar)Lys.sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, D(6ANic) Lys.sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, D(6ANic) Orn.sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDQal.sup.1, DCpa.sup.2, DPal.sup.3, cisPzACAla.sup.5, DPicLys.sup.6, NLeu.sup.7, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DCpa.sup.2, DPal.sup.3, DPicLys.sup.5, DAPhe(PicSar).sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDQal.sup.1, DCpa.sup.2, DPal.sup.3, PicLys.sup.5, DPal.sup.6, ILys.sup.8, DAla.sup.10)LHRH
(NAcDNal.sup.1, DCpa.sup.2, DPal.sup.3, PicLys.sup.5, DOrn (ACyp).sup.6, ILys.sup.8, DAla.sup.10)LHRH
DESCRIPTION OF THE PREFERRED EMBODIMENTS
LHRH analogs and congeners with high water solubility have been synthesized. These new analogs had 0%-100% antiovulatory activity at a 0.5 .mu.g dosage and 0%-80% at 0.25 .mu.g. The ED.sub.50 for histamine release was 30.5 .mu.g/ml->300 .mu.g/ml.
The compounds of the present invention are water soluble and some of them, as listed below, are also soluble in physiological salt solution without gelling. The preferred analogs show favorable potency and histamine values. These are important for clinical use and are not easily accomplished.
An important final point is that many LHRH analogs of the present invention do not contain the PzACAla substitution and still have favorable potency, histamine releasing effects and water solubility as well as solubility in physiological salt solution without gelling, except as listed below. The reason this is important is because the PzACAla is difficult to synthesize because of the stereochemical requirements, thus adding substantially to the expense of synthesis in large amounts. A concerted effort was made to eliminate the PzACAla for this very reason. As you know emphasis on the cost of drugs is a major issue these days.
Analogs 27316, 27345, 27234, 27459, and 27490 were soluble at 1 mg/ml in the physiological buffer solution PIPES-AC. Analog 27249 (1 mg/ml) and analog 27355 (0.3 mg/ml were slightly turbid. Analog 27274 gelled slightly at 1 mg/ml and analog 27163 gelled slightly at 1 mg/ml. For comparison, Antide gelled completely at 1 mg/ml while Lystide did not.





The following Examples are included to show the best mode of the present invention and not to limit the invention unless specifically claimed.
EXAMPLE 1
Design, Synthesis and Biological Evaluation of Analogs of LHRH by Criteria of Potency, Safety and Solubility
Experimental
Materials
BOC-Ser(OBz1), BOC-Pro, BOC-Leu and BOC-D-Ala were obtained from Peninsula Laboratories, Belmont, CA. Abu was obtained from Sigma Chemical Company, St. Louis, Mo and it was converted to the BOC-derivative using standard procedures (Moroder et al.).
BOC-D-2-Nal, BOC-D-pClPhe, BOC-D- and L-3-Pal, BOC-ILys(Z) dicyclohexylamine salt, BOC-D- and L-NicLys and BOC-D-3-Qal were all provided by Dr. Narasimha Rao of the Southwest Foundation for Biomedical Research, San Antonio, Tex. BOC-D- and L-PzAla were synthesized by literature methods (Peterman et al.).
BOC-AOPP was kindly provided by Dr. R. M. Freidinger, Merck, Sharp & Dohme, West Point, Pa. (Freidinger et al., 1980 and 1982). .alpha.-BOC-cis-D- and L-4-amino-cyclohexylalanine were provided by Dr. Narasimha Rao, and were converted to the corresponding BOC-cis-D-and L-PzACAla derivatives by acylation with the p-nitrophenylester (Folkers et al., 1990) of pyrazinecarboxylic acid from the Aldrich Chemical Company, Milwaukee, Wis., in DMF.
BOC-D- and L-PicLys were similarly prepared from .alpha.-BOC-D-and L-Lys and picolinic acid p nitrophenylester (Ljungqvist et al.) in DMF. .alpha.-BOC-D- and L-(DSer)Lys, protected at the D-Ser moiety by the Z group at the amino group and by the benzyl group at the hydroxyl function, were synthesized by acylation of BOC-D- and L-Lys in DMF by Z-D-Ser(OBzl)-ONp in the presence of 1-hydroxybenzotriazole. The BOC-D-(AcDSer)Lys, protected as the benzyl derivative at the D-Ser hydroxy, was similarly prepared by the reaction between Ac-D-Ser(OBzl)ONp and BOC-D-Lys.
All synthesized amino acids and intermediates were characterized by NMR and were homogenous on TLC.
The benzhydrylamine hydrochloride resin was purchased from Beckman Bioproducts, Palo Alto, Calif. The dicyclohexylcarbodiimide was from Aldrich and was distilled in vacuo before use. The dichloromethane was distilled from sodium carbonate. All other solvents and reagents were reagent grade.
Synthesis
The peptides were synthesized by the solid-phase method using a Beckman automated 990 peptide synthesizer. The protocol details used were essentially as described (Folkers et al., 1984). The peptide was cleaved from the resin with concomitant removal of all protecting groups by treatment with doubly distilled HF at 0.degree. C. for 1 h in the presence of about 10% anisole in p-cresol. The HF was then evaporated, in vacuo, first by a water aspirator and then by pump vacuum overnight. The residue was then extracted 2-3 times with ether in order to remove non-peptidic material. The crude peptide was subsequently extracted with aqueous acetic acid and the extract was lyophilized.
Purification and Characterization
Purification was achieved by chromatography on SiO.sub.2 (EM, 230-400 mesh) with the solvent system n-butanol:acetic acid:water 4:1:2 or 4:1:5 (upper phase) followed by gel filtration on Sephadex G 25 with 6% aqueous acetic acid as the eluant. An alternative purification method was gel filtration as above followed by chromatography on Sephadex LH 20 with the solvent system water:butanol:acetic acid:methanol 90:10:10:8.
The purity was checked by TLC, amino acid analysis and HPLC.
Amino acid analyses were carried out on a Beckman 118 CL amino acid analyzer after hydrolysis in constant boiling HCl for 24 h using standard procedures (Folkers et al., 1984). The unnatural amino acids were qualitatively determined with the exception of 3-Pal which was quantified.
The purity was further checked by HPLC using a Waters instrument with a 660 solvent programmer and a Vydac C.sub.18 column. The flow rate was 1.5 ml/min and the absorbance was recorded at 210 nm. Different gradients of increasing concentration of acetonitrile in 0.01M KH.sub.2 PO.sub.4, adjusted to pH 3 with H.sub.3 PO.sub.4, were employed. All peptides were estimated to be 97-99% pure in this system (data not shown).
Biological assays
AOA in rats was determined as reported (Humphries et al.). The histamine release was assayed in rat mast cells as reported (Hook et al., Karten et al., 1987). The ED.sub.50 value reported is the concentration in .mu.g/ml that releases 50% of total releasable histamine. The biological data are in the following table. These are more completely explained in U.S. Pat. No. 4,935,491 that is incorporated, in pertinent part, by reference herein.
__________________________________________________________________________LHRH ANALOGS, STRUCTURE AND BIOLOGICAL ACTIVITY AOA % INHIB HISTAMINE DOSAGE (#OVUL .mu.g/mlCPD # .mu.g #RATS) ED.sub.50 STRUCTURE*__________________________________________________________________________27163 0.25 100 (0/5) 40 .+-. 0 (NAcDQal.sup.1, DPtf.sup.2, DPal.sup.3, cisPzACAla.sup.5, 0.125 22 (7/9) DPucKts.sup.6, DAla.sup.10) LHRH27234 0.5 100 (0/6) 104 .+-. 4 (NAcDNAl.sup.1, DpClPhe.sup.2, DPal.sup.3, cisPzACAla.sup.5, 0.25 67 (2/6) DNicLys.sup.6, ILys.sup.8, DAla.sup.10) LHRH27249 0.5 100 (0/5) 95 .+-. 5 (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, Thr.sup.4, PicLys.sup.5, 0.25 17 (5/6) DPicLys.sup.6, ILys.sup.8, DAla.sup.10) LHRH27274 0.5 100 (0/5) 30.5 .+-. 2 (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, 0.25 17 (5/6) DPicLys.sup.6, Thr.sup.7, ILys.sup.8, DAla.sup.10) LHRH27306 0.5 20% 100 .+-. 0 (NapDthr.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, DPicLys.sup.6, ILys.sup.8, DAla.sup.10) LHRH27313 0.5 0 298 .+-. 3 (NacDNAl.sup.1, DpClPhe.sup.2, DPal.sup.3, NicLys.sup.5, DNicLys.sup.6, Thr.sup.7, ILys.sup.8, DAla.sup.10) LHRH27315 0.5 0 100 .+-. 0 (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, Thr.sup.4, NicLys.sup.5, DNicLys.sup.6, Thr.sup.7, ILys.sup.8, DAla.sup.10) LHRH27316 0.5 100 (0/5) 34 .+-. 2 (NacDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicyLys.sup.5, 0.25 50 (5/10) D (PicSar) Lys.sup.6, ILys.sup.8, DAla.sup.10) LHRH27345 0.5 100 (0/10) 31.5 .+-. 2 (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, D (PicSar) Lys.sup.6, 0.25 33 (4/6) ILys.sup.8, DAla.sup.10) LHRH27355 0.5 83 (1/6) 96 .+-. 0 (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, 0.25 50 (3/6) D (6ANic) Lys.sup.6, ILys.sup.8, DAla.sup.10) LHRH27459 0.5 100 (0/6) 100 .+-. 0 (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, 0.25 25 (3/4) D (6ANic) Orn.sup.6, ILys.sup.8, DAla.sup.10) LHRH27490 0.5 100 (0/5) 200 .+-. 100 (NacDQal.sup.1, DCpa.sup.2, DPal.sup.3, cisPzACAla.sup.5, 0.25 80 (2/10) DPicLys.sup.6, NLeu.sup.7, ILys.sup.8, DAla.sup.10) LHRH27535 0.5 86% 275 .+-. 25 (NAcDNAl.sup.1, DCpa.sup.2, DPal.sup.3, DPicLys.sup.5, 0.25 20% DAPhe (PicSar).sup.6, ILys.sup.8, DAla.sup.10) LHRH27542 0.5 100% >300 (NAcDQal.sup.1, DCpa.sup.2, DPal.sup.3, PicLys.sup.5, 0.25 0% DPal.sup.6, ILys.sup.8, DAla.sup.10) LHRH27629 0.5 100% NA (NAcDNal.sup.1, DCpa.sup.2, DPal.sup.3, PicLys.sup.5, 0.25 17% DOrn (ACyp).sup.6, ILys.sup.8, DAla.sup.10)__________________________________________________________________________ LHRH *Unspecified positions are from LHRH (pGlu.sup.1, His.sup.2, Trp.sup.3, Ser.sup.4, Tyr.sup.5, Gly.sup.6, Leu.sup.7, Arg.sup.8, Pro.sup.9, Gly.sup.10 --NH.sub.2)
The following citations are incorporated in pertinent part by reference herein for the reasons cited in the above text.
References
Folkers et al., (Jun. 19, 1990) U.S. Pat. No. 4,935,491.
Folkers et al., Biochem, Biophys. Res. Commun. 123, 1221 (1984).
Freidinger et al., Since 210, 656 (1980)
Freidinger et al., J. Org. Chem. 47 104 (1982).
Humphries et al., Biochem. Biophys. Res. Comm. (1976) 72, 939-944.
Humphries et al., (1987) J. Med. Chem. 21, 120-123.
Janecka et al. (1994) Amino Acids 6,111-130.
Karten et al., (1986) Endocr. Rev. 7: 44-66.
Karten et al., in: LHRH and its Analogs: Contraceptive and Therapeutic Applications II, pp. 179-190, MTP Press LTD, Lancaster, England (1987).
Ljungqvist et al., (1988) Acta Chem. Scand. 842: 408-410.
Ljungqvist et al., (1987) Biochem. Biophys Res. Comm. 148: 849-856.
Ljungqvist et al., Proc. Natl. Acad. Sci. USA 85, 8236 (1988).
Moroder et al., Hoppe-Seyler's Z. Physiol. Chem. 357, 1651(1976).
Peterman et al., Helv. Chim. Acta 66, 1513 (1983).
Rao et al., (1987) Int. J. Peptide Protein Res. 29: 118-125.
Schmidt et al., (1984) Contraception 29: 283-289.
Sundaram et al., Agents and Actions 25, 307 (1988).
Claims
  • 1. An LHRH analog having the formula
  • (NAcDQal.sup.1, DPtf.sup.2, DPAl.sup.3, cisPzACAla.sup.5, DPicLys.sup.6, DAla.sup.10)LHRH.
  • 2. An LHRH analog having the formula
  • (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, cisPzACAla.sup.5, DNicLys.sup.6, ILys.sup.8, DAla.sup.10)LHRH.
  • 3. An LHRH analog having the formula
  • (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, Thr.sup.4, PicLys.sup.5, DPicLys.sup.6, ILys.sup.8, DAla.sup.10)LHRH.
  • 4. An LHRH analog having the formula
  • (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, DPicLys.sup.6, Thr.sup.7, ILys.sup.8, DAla.sup.10)LHRH.
  • 5. An LHRH analog having the formula
  • (NapDThr.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, DPicLys.sup.6, ILys.sup.8 , DAla.sup.10)LHRH.
  • 6. An LHRH analog having the formula
  • (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, NicLys.sup.5, DNicLys.sup.6, Thr.sup.7, ILys.sup.8, DAla.sup.10 )LHRH.
  • 7. An LHRH analog having the formula
  • (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, Thr4NicLys.sup.5, DNicLys.sup.6, Thr.sup.7, ILys.sup.8, DAla.sup.10)LHRH.
  • 8. An LHRH analog having the formula
  • (NAcDNal.sup.1 , DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, D(PicSar)Lys.sup.6, ILys.sup.8, DAla.sup.10)LHRH.
  • 9. An LHRH analog having the formula
  • (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, D(PicSar)Lys.sup.6, ILys.sup.8, DAla.sup.10)LHRH.
  • 10. An LHRH analog having the formula
  • (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, D(6ANic) Lys.sup.6, ILys.sup.8, DAla.sup.10)LHRH.
  • 11. An LHRH analog having the formula
  • (NAcDNal.sup.1, DpClPhe.sup.2, DPal.sup.3, PicLys.sup.5, D(6ANic )Orn.sup.6, ILys.sup.8, DAla.sup.10)LHRH.
  • 12. An LHRH analog having the formula
  • (NAcDQal.sup.1, DCpa.sup.2, DPal.sup.3, cisPzACAla.sup.5, DPicLys.sup.6, NLeu.sup.7, ILys.sup.8 , DAla.sup.10)LHRH.
  • 13. An LHRH analog having the formula
  • (NAcDNal.sup.1, DCpa.sup.2, DPal.sup.3, DPicLys.sup.5, DAPhe(PicSar).sup.6, ILys.sup.8, DAla.sup.10)LHRH.
  • 14. An LHRH analog having the formula
  • (NAcDQal.sup.1, DCpa.sup.2, DPal.sup.3, PicLys.sup.5, DPal.sup.6, ILys.sup.8, DAla.sup.10)LHRH.
  • 15. An LHRH analog having the formula
  • (NAcDNal.sup.1, DCpa.sup.2, DPal.sup.3, PicLys.sup.5, DOrn(ACyp).sup.6, ILys.sup.8, DAla.sup.10)LHRH.
Parent Case Info

This is a continuation-in-part of application Ser. No. 07/946,056 filed Sep. 15, 1992 now U.S. Pat. No. 5,480,969 which is incorporated by reference herein.

Government Interests

This invention was made with government support under contract no. N01-HD-1-3101 awarded by the National Institutes of Health. The government, therefore, has certain rights in the invention.

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Entry
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Continuation in Parts (1)
Number Date Country
Parent 946056 Sep 1992