Patent Application
20240317802
The present invention relates to the technical field of bioactive peptides, in particular to an anti-aging sturgeon roe tetrapeptide and a preparation method and application thereof.
Skin aging is the external manifestation of human aging. A mechanism of aging is complex. Research in recent years has shown that the pathogenesis may be related to many factors, including lipid metabolism, oxidative stress, inflammation, apoptosis, etc. Research has shown that the main reason for skin aging is that the oxygen free radicals produced by ultraviolet rays and other factors affect the normal growth cycle of skin cells, promote the hydrolysis of collagen and other extracellular matrix by protease, and cause DNA damage and the reduction of matrix protein synthesis, thereby resulting in skin relaxation and reduction of elasticity. With the stimulation of facial muscle fibers by expressions, the most significant sign of skin aging, i.e., wrinkles are produced.
Mechanism of endogenous skin aging: endogenous aging of skin is an irreversible and slow physiological process. Endogenous skin aging is evident only after a certain stage of age, and is characterized by skin drying, roughness, elasticity reduction, and the production of wrinkles. With the increase of age, the dermal mast cells and fibroblasts in the skin tissue are decreased, the secretion of collagen is decreased, and the dermo-epidermal junction area is flattened. Under the influence of aging of other organs in a human body, endogenous skin aging is caused by many factors. From the perspective of the physiological mechanism, oxidative stress causes damage to DNA, protein and other components of cells, and exacerbates progressive telomere shortening, which is an important reason for endogenous skin aging.
Exogenous skin aging: exogenous factors, such as ultraviolet irradiation, pollution and smoking, produce a series of reactions such as pro-oxidation/anti-oxidation through neuroendocrine immune regulation, and affect cell renewal, so as to permanently affect the physiological function of the skin. Ultraviolet induced photoaging is the currently recognized most important cause of skin aging.
Reactive oxygen species (ROS) produced by UV induction can damage DNA and inhibit tyrosine phosphatases, which leads to signal transduction enhancement and ultimately leads to up-regulation of the transcription factor AP-1. At the same time, ultraviolet ray can also lead to the up-regulation of c-Jun as one of the components of AP-1, and down-regulation of retinoic acid receptors, to further weaken the inhibitory effect of retinoic acid on AP-1. Moreover, ultraviolet ray directly induces DNA variation, up-regulates nuclear factor-kB (NF-kB), and inhibits transforming growth factor-β (TGF-β)-mediated cell signaling pathways. These effects lead to degradation or secretion decrease of collagen. Collagen as the most widespread matrix protein of the body provides the support and elasticity for skin. Once the balance of collagen secretion/degradation is broken, the content of collagen in the skin will be reduced, thereby affecting the stability of the skin structure. With the excessive stimulation of the fiber by muscle movement such as expression muscle, the most significant sign of skin aging, i.e., wrinkles are produced. Therefore, the synergistic use of active ingredients with multiple action mechanisms is a preferred choice to achieve a desired anti-aging effect.
Functional extracts separated from aquatic food can be used as functional food and nutritional health products. Peptides obtained from aquatic protein by biotechnology means not only show high nutritional value, but also show biological characteristics for diet or therapeutic purposes, have special active aquatic extracts, and may become functional food for human nutrition. Fish is the earliest biological resource that people begin to eat and rich in protein, vitamins and minerals, and is a high-quality raw material for the development of functional foods such as oligopeptide. At present, a large number of antioxidant, antitumor, antibacterial and anti-inflammatory polypeptides have been obtained by enzymolysis and separation from silver carp, Pacific saury, tilapia and the like, and new structures and mechanisms of action have been continuously discovered. Therefore, rational and efficient development and utilization of freshwater fish resources has broad market prospects, and has certain social and economic significance.
However, there is no relevant report on the research of anti-aging effect of sturgeon roe polypeptide.
Therefore, how to prepare a sturgeon roe polypeptide with anti-aging effect is an urgent problem for those skilled in the art.
In view of this, a purpose of the present invention is to provide an anti-aging sturgeon roe tetrapeptide and a preparation method and application thereof, so as to solve the deficiencies in the prior art.
In order to achieve the above purpose, the present invention adopts the following technical solution:
An anti-aging sturgeon roe tetrapeptide has a peptide sequence of NLPL.
A preparation method of the anti-aging sturgeon roe tetrapeptide specifically comprises the following steps:
Further, in the above step (1), a mass ratio of the sturgeon roes to the deionized water is 1:6.
Further, in the above step (1), rotational speed of homogenizing is 8000 rpm and time is 1 min.
Further, in the above step (2), an addition amount of the Alcalase is 1%.
Further, in the above step (2), temperature of enzymolysis is 55° C. and time is 8 h.
Further, in the above step (2), temperature of enzyme inactivation is 90-100° C. and time is 10-15 min.
Further, in the above step (2), cooling is performed to room temperature.
Further, in the above step (3), temperature of centrifugal treatment is 4° C., rotational speed is 8000 rpm, and time is 15 min.
Further, in the above step (3), temperature of storage is −80° C.
The present invention further requests to protect an application of the above sturgeon roe tetrapeptide or the sturgeon roe tetrapeptide prepared by the above preparation method in preparation of anti-aging drugs, anti-aging food, anti-aging health products or anti-aging cosmetics.
According to the above technical solution, compared with the prior art, the present invention has the following beneficial effects:
The present invention evaluates the protein content, hydrolysis degree, protein recovery rate, ABTS antioxidant activity and tyrosinase inhibitory activity of sturgeon roe peptide, selects the enzyme addition amount of 1% (according to substrate mass), enzymolysis time of 8 h and solid-liquid ratio of 1:6, and selects the Alcalase as the preparation condition of a sturgeon roe peptidyl delivery system. The obtained enzymolysis product of the sturgeon roes has high lipid content.
The sturgeon roe peptide prepared by the present invention can improve the levels of oxidative stress and skin related factors (type I collagen and hyaluronic acid) by adjusting a cell proliferation state, so as to realize the protective effect on skin cells at a cellular level.
According to the results of animal experiments, the sturgeon roes prepared by the present invention can realize the anti-aging effect by adjusting the oxidative stress and the skin state in small aging water bodies.
Based on a virtual selection means, the present invention finds that the anti-aging tetrapeptide NLPL derived from sturgeon can play an important anti-aging role in the body, has high absorption efficiency, and can play an important role in the body.
Technical solutions in the embodiments of the present invention are described clearly and fully below. Apparently, the described embodiments are merely part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those ordinary skilled in the art without contributing creative labor will belong to the protection scope of the present invention.
A preparation method of anti-aging sturgeon roe tetrapeptide specifically comprises the following steps:
A preparation method of anti-aging sturgeon roe tetrapeptide specifically comprises the following steps:
A preparation method of anti-aging sturgeon roe tetrapeptide specifically comprises the following steps:
The research aims to optimize the hydrolysis conditions of sturgeon roes to obtain bioactive peptide. The experimental design covers the changes of different enzyme types, enzymolysis time, enzyme addition amount and solid-liquid ratio.
Firstly, 10 g of sturgeon roes are taken, added with ultra-pure water and homogenized for 1 min at 8000 rpm. Then, the optimal pH value is adjusted, enzymes are added, and a sample is hydrolyzed in a thermostatic oscillator at 55° C. Subsequently, the sample is placed in the boiling water bath for enzyme inactivation at 90-100° C. for 10-15 min. After cooling to room temperature, centrifugal treatment is carried out at 4° C. and 8000 rpm for 15 min. The supernatant is taken for the subsequent determination of indexes, and then stored at −80° C. for use.
Single factor investigation of enzyme type: in the single factor research of enzyme type, the addition amount of enzyme is 1%, the hydrolysis time is 4 h, and the solid-liquid ratio is 1:6. The effects of different proteases (trypsin, Alcalase and complex enzyme with trypsin-Alcalase 1:1) on hydrolysis are tested.
Single factor investigation of enzymolysis time: another single factor research aims to determine the effect of the enzymolysis time on peptide production. Under the condition that the solid-liquid ratio is 1:6 and the addition amount of enzyme is 1%, Alcalase is used for hydrolysis, and the hydrolysis time is set as 2 h, 4 h, 8 h, 12 h and 16 h, respectively.
Single factor investigation of addition amount of enzyme: the influence of the addition amount of enzyme on hydrolysis effect is evaluated through single factor research. The solid-liquid ratio is kept at 1:6; Alcalase is used for hydrolysis for 8 h; and 0.2%, 0.5%, 0.8%, 1% and 2% of addition amount of enzyme are added respectively.
Single factor investigation of solid-liquid ratio: finally, the influence of the solid-liquid ratio on hydrolysis effect is investigated by single factor analysis. The sturgeon roe samples are mixed with 20 mL, 40 mL and 60 mL of water respectively, and then hydrolyzed with 1% Alcalase for 8 h.
The degree of hydrolysis is determined by OPA method, and the protein content of fish roe and enzymolysis supernatant is determined by Kjeldahl method in GB 5009.5-2016 National Food Safety Standard-Determination of Protein in Foods.
Protein recovery rate %=protein content of enzymolysis supernatant/protein content of fish roe×100%.
A method for determining ABTS free radical scavenging capacity is an experimental method for evaluating the antioxidant capacity of a compound or sample. ABTS (2, 2′-azino-bis(3-ethylbenzothiazoline) is a synthetic free radical compound that can be used to simulate oxygen free radicals in living organisms. In the method, the antioxidant performance of the compound is judged by measuring the scavenging ability of the compound for ABTS free radicals. In the determination process, ABTS free radicals will change the color, and the addition of antioxidants will cause color fading, the degree of which is in direct proportion to the antioxidant capacity. The basic steps of the method include:
In this experiment, the ABTS free radical scavenging ability of the enzymolysis supernatant is determined with reference to the optimized method.
L-tyrosine solution: weighing 0.025 g of L-tyrosine into 50 mL of sterile ionized water, dissolving with a phosphate buffer solution, and fixing a volume to 50 mL, for use right after it is ready.
Tyrosinase solution: preparing the tyrosinase with the phosphate buffer solution at 1000 U/mL and storing away from light at −20° C., for use right after it is ready.
Sample solution: diluting the sample with PBS buffer salt solution to a concentration of 1 mg/mL, for use right after it is ready.
By referring to the addition amounts of reagents in Table 1, the L-tyrosine solution, the sample solution/reagent and the phosphate buffer solution are added into the reaction system successively, thoroughly mixed, incubated in a constant temperature environment at 37° C. for 10 min, then added with 20 μL of tyrosinase solution into each well successively, and uniformly mixed at 37° C. to react for 5 min. A microplate reader is immediately put and the absorbance is tested at 475 nm.
The degree of hydrolysis and protein recovery rate of enzyme types are shown in
It can be seen from
The ABTS free radical scavenging activity of the enzyme types are shown in
It can be seen from
The tyrosinase inhibitory activity of the enzyme types is shown in
It can be seen from
The protein recovery rate and the degree of hydrolysis at enzymolysis time are shown in
It can be seen from
The ABTS free radical scavenging activity at enzymolysis time is shown in
It can be seen from
Tyrosinase inhibitory activity at enzymolysis time is shown in
It can be seen from
The degree of hydrolysis and the protein recovery rate of the addition amount of enzyme are shown in
It can be seen from
The ABTS free radical scavenging activity of the addition amount of enzyme is shown in
It can be seen from
The tyrosinase inhibitory activity with the addition amount of enzyme is shown in
It can be seen from
The degree of hydrolysis and the protein recovery rate of the solid-liquid ratio are shown in
It can be seen from
The ABTS free radical scavenging activity of the solid-liquid ratio is shown in
It can be seen from
The tyrosinase inhibitory activity of the solid-liquid ratio is shown in
It can be seen from
A 250 mL flat-bottomed flask is made at constant weight and weighed. The sample is weighed into a 50 mL colorimetric tube, added with 2 mL of 95% ethanol and 4 mL of water, and mixed uniformly. 10 mL of hydrochloric acid solution is added and mixed uniformly. The colorimetric tube is placed in a water bath at 70-80° C. and hydrolyzed for 40 min. The flask is oscillated every 10 min to mix the particles attached to the flask wall into the solution. After hydrolysis, the colorimetric tube is taken out and cooled to room temperature.
10 mL of 95% ethanol is added and mixed uniformly. 30 mL of ether and petroleum ether mixture is added, covered, shaken for 5 min and stood for 10 min. The ether layer extract is collected into a 250 mL flask. The hydrolysate is extracted repeatedly for 3 times according to the above steps, and the ether layer is steamed in a water bath. The residue is the fat extract. The flask is dried in an oven at 60° C. to constant weight and weighed.
The fat contents of sturgeon roes and freeze-dried enzymolysis solution are shown in Table 2.
4 mL of 0.5 mol/L sodium methanol is added to the flask and heated in a water bath at 45° C. for 20 min. The sample liquid in the flask is transferred into a 20 mL colorimetric tube and 4 mL of 14% boron trifluoride methanol solution is added and heated in a water bath at 45° C. for 20 min. The colorimetric tube is cooled to room temperature, added with 3 mL of n-hexane to extract for 2 min, and stood for stratification; then, n-hexane layer is taken for measurement.
The initial temperature of the injection volume is 100° C. for 13 min; the temperature is raised to 180° C. at 10° C./min for 6 min, raised to 192° C. at 1° C./min for 9 min, and raised to 240° C. at 4° C./min for 2 min. Chromatographic column operation conditions are as follows: sample volume is 1 μL; a shunt ratio is 20:1; the injection temperature is 260° C.; ion source temperature is 240° C.; SCAN full scan mode; and a scan range is 40 to 400. The result is expressed in g/100 g.
The results of unsaturated fatty acid in sturgeon roes and enzymolysis solution are shown in Table 3.
It can be seen from Table 3 that the contents of Ω3, Ω6 and Ω9 fatty acids in the sturgeon roe enzymolysis solution are higher than the content of fatty acid in sturgeon roes. The total content of unsaturated fatty acid per 100 g of enzymolysis solution is more than twice that of sturgeon roes per 100 g.
The protein content, degree of hydrolysis, protein recovery rate, ABTS antioxidant activity and tyrosinase inhibitory activity of the sturgeon roe peptide are evaluated. It is selected that the enzyme addition amount is 1% (according to substrate mass), enzymolysis time is 8 h, and the solid-liquid ratio is 1:6, and Alcalase is selected as the preparation condition of a sturgeon roe peptidyl delivery system.
HaCaT cells at logarithmic growth stage are selected; the cell concentration is adjusted to 1.5×104 cells/mL; and the cells are inoculated into a 96-well plate. The cells are divided into a normal group and sample groups with different concentration gradients. The normal group is given a blank medium and a sturgeon roe extract (protein concentrations are 0.1, 0.3, 0.5, 0.9, 1.3, and 1.7 mg/mL, respectively). Six parallels are set for each concentration and cultured for 24 h. OD values of the groups are measured by CCK-8 method, and relative cell viability is calculated.
Relative cell viability %=ODsample/ODnormal×100%.
HaCaT cells are inoculated into a 96-well plate with a concentration of 1.5×104 cells per well, with 100 μL per well. The cells are attached to the wall in an incubator at 37° C. and 5% CO2 for 24 h, and the medium is absorbed and discarded. The cells are divided into a normal group and an H2O2 damage group (0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, and 1.3 mmol/L). Six compound wells are set in each group and incubated at 37° C. and 5% CO2 for 2 h. OD values of the groups are measured by CCK-8 method, and relative cell viability is calculated.
Relative cell viability %=ODdamage/ODnormal×100%.
HaCaT cells are inoculated into a 96-well plate with a concentration of 1.5×104 cells per well, with 100 μL per well. The cells are attached to the wall in an incubator at 37° C. and 5% CO2 for 24 h, and the medium is discarded. The cells are divided into a normal group, a model group and a drug administration group. The normal group and the model group are given a blank medium, and the drug administration group is added with samples of different concentration gradients. Six compound wells are set in each group, and incubated at 37° C. and 5% CO2 for 24 h. The supernatant is sucked and discarded. Except that the normal group is added with the blank medium, 900 μmol/L H2O2 is added to the model group and the drug administration group for 6 h. OD values of the groups are measured by CCK-8 method, and relative cell viability is calculated.
HaCaT cells are inoculated into a 6-well plate at a concentration of 6×105 cells per well, and attached to the wall in an incubator at 37° C. and 5% CO2 for 24 h. The medium is discarded. The cells are divided into a normal group, a model group and a drug administration group. The normal group and the model group are given a blank medium, and samples of different concentration gradients are added to the drug administration group. The cells are incubated at 37° C. and 5% CO2 for 24 h, and the supernatant is absorbed and discarded. Except that the blank medium is added to the normal group, 900 μmol/L H2O2 is added to the model group and the drug administration group for 6 h. The cells are scraped off with a cell scraper and broken for later use.
1.0×106 cells are transferred into a 5 mL flow test tube, and centrifuged at 300 g at room temperature for 5 min. The centrifuge liquid is removed; 2 mL of binding buffer is added, and centrifuged at 300 g at room temperature for 5 min. The supernatant is removed. 100 μL of stain buffer resuspension cells are added, and 5 μL of Annexin V APC and 5 μL of PI solution are added, and incubated at room temperature for 25 min. 100 μL of stain buffer is added and tested on the computer.
The cell treatment mode is consistent with “2.3 cell apoptosis detection”. The cells are scraped off with a cell scraper and broken for later use. The indexes are detected by operation according to the relevant steps of kits GSH (glutathione), SOD (superoxide dismutase), MDA (malondialdehyde) and LDH (lactate dehydrogenase).
HaCaT Human Immortalized Epidermal Cells (HDF Human Dermal Fibroblast cells are inoculated by 1×106 per well) are inoculated by 1.2×106 per well. After the cells are attached to the wall, straight lines are drawn in a 6-well plate with a 200 μL gun head. The cells are divided into a normal group and a sturgeon roe extract (protein concentration) by 0.4 mg/mL. The normal group is added with a basic medium. A sample adding group is added with a sample solution configured by the basic medium. Cell growth conditions are observed at 0 h, 12 h and 24 h respectively.
HaCaT Human Immortalized Epidermal Cells and HDF Human Dermal Fibroblast cells are inoculated on a 12-well plate at 3×105 per well, and attached to the wall for 24 h. Then, a normal medium/sample is added (two concentration gradients are set). After 24 h of culture, the culture supernatant is collected for detection (Human hyaluronic acid (HA) ELISA kit from Nanjing Jiancheng, and human Type I collagen (Col I) ELISA kit).
The screening of the molding concentration is shown in
It can be seen from
Results are shown in
Wherein the cytotoxicity of the enzymatic hydrolysate of sturgeon is shown by A in
It can be seen from A in
The protective effect of the enzymolysis product of sturgeon roes on HaCaT cells with H2O2 damage is shown by B in
It can be seen from B in
Results are shown in Table 4 and
It can be seen from Table 4 that different quadrants of the flow cytometer represent different cell states.
It can be seen from
Results are shown in
GSH is a coenzyme of many enzymes such as GSH-Px, which is involved in scavenging HO, O2−, H2O2, etc., and capable of effectively protecting the body from oxidative stress damage. Therefore, the amount of GSH can reflect the antioxidant capacity of the body to a certain extent.
The effect of enzymatic hydrolysate of sturgeon on glutathione (GSH) level of damaged cells is shown by A in
It can be seen from A in
SOD can convert harmful superoxide free radicals into H2O2 through cell respiration. Cell damage can lead to the change of the oxidative stress level. In order to further explore the reasons, the project team detects the activity of superoxide dismutase SOD in cells.
The effect of enzymatic hydrolysate of sturgeon on superoxide dismutase (SOD) of damaged cells is shown by B in
It can be seen by B in
The results show that the sturgeon roe extract can achieve the purpose of improving the oxidative stress state of cells by improving the SOD activity.
Malondialdehyde (MDA) is one of the iconic products of lipid peroxidation, and the content of MDA in the organism can directly reflect the degree of oxidation or damage of the organism. Therefore, improving the MDA level of the body is also one of the important means to achieve oxidation resistance and maintain cell vitality.
The effect of enzymatic hydrolysate of sturgeon on malondialdehyde (MDA) in damaged cells is shown by C in
It can be seen from C in
The damage of the cell membrane structure caused by apoptosis or necrosis results in the release of enzymes in the cytoplasm into a culture solution, including lactate dehydrogenase (LDH) which has stable enzyme activity. Quantitative analysis of cytotoxicity can be achieved by detecting LDH activity.
The effect of enzymatic hydrolysate of the sturgeon on lactate dehydrogenase (LDH) in damaged cells is shown by D in
It can be seen from D in
Based on the above experimental results, the protein concentration 0.4 mg/mL of the sturgeon roe extract is selected for a cell scratch experiment.
HDF cells are the abbreviation of Human Dermal Fibroblast cells. This is a cell type that exists in the dermis of human skin and is mainly responsible for synthesizing collagen, elastic fibers and other extracellular matrix molecules and maintaining the structure, elasticity and stability of the skin. HDF cells play an important role in physiological and pathological processes such as skin healing, wound repair and skin aging. Because of their key role in skin health and diseases, HDF cells are often used by researchers to explore cell biology, molecular mechanisms, and the pathogenesis of related diseases.
HaCaT cells belong to a human skin epithelial cell line, also known as Human Keratinocyte Cells (HaCaT). The cell line is isolated and cultured from a normal human skin keratosis. HaCaT cells have high proliferative activity in culture in vitro and can constantly divide to form multi-layer cell accumulation, similar to the epidermal layer of human skin. Because of the characteristic of the HaCaT cells to simulate skin epithelial cells, the HaCaT cells are often used in skin biology research, such as cell proliferation, differentiation, keratinization and skin diseases. The HaCaT cells can also be widely used in drug safety evaluation, cosmetic testing, and biomedical research to explore the molecular mechanisms of skin health and diseases.
Results are shown in
It can be seen from A in
It can be seen from B in
It can be seen from E in
It can be seen from C in
It can be seen from F in
The samples are analyzed by LC-MS/MS equipped with an online nanojet ion source. 3 μL of samples are loaded, and the samples are separated with a gradient of 60 min. The column flow rate is controlled at 300 nL/min, the column temperature is 40° C., and the electrospray voltage is 2 kV. The gradient starts from 2% B-phase, rises to 35% with a nonlinear gradient in 47 min, and rises to 100% within 1 min for 12 min.
A mass spectrometer is operated in a data-dependent collection mode, and automatically switched between MS and MS/MS collection. Mass spectrum parameters are set as follows: (1) MS: scanning range (m/z): 200-2000; resolution: 70,000; AGC target: 3e6; maximum injection time: 50 ms; (2) HCD-MS/MS: resolution: 17,500; AGC target: 1e5; maximum injection time: 45 ms; collision energy: 28%; and dynamic exclusion time: 30 s.
Setting of database search parameters: a tandem mass spectrometry is analyzed by PEAKS Studio version 10.6. PEAKS DB searches a database for uniprot-Salmo salar or uniprot-Acipenser sinensis and sets none enzymolysis. An allowable error of fragment ion mass of database search parameters is 0.02 Da, and an allowable error of parent ion mass is 7 ppm. The protein calorie value is 1 unique peptide. The peptide caloric value is −10 log P≥20.
The database of bioactive peptide and a computer virtual screening tool are used to analyze, virtually screen and predict the potential bioactive peptide in the peptide sequence of the enzymolysis product.
The distribution of all target polypeptide sequences obtained by the mass spectrometer is shown by using Upset Venn diagram.
The potential biological activity of peptide fragments is predicted and ranked by Peptide Ranker, and cell permeability is predicted and ranked by CPPpred, which can evaluate the cell penetration potential of peptide. Both scores are between 0 and 1. The higher the score is, the greater the potential is.
The peak area of the peptide represents the content to some extent, and thus can be used as a filtering condition to improve the accuracy of the identified peptide fragment and screen the major contributor peptide fragment. Therefore, the potential bioactive peptide is screened by taking the filtering conditions of Peptide Ranker score >0.5, CPPpred score >0.1, and relative peak area >0.05%. A bubble diagram is made by taking the Peptide Ranker score as an x-axis, the CPPpred score as a y-axis, and the peak area of the peptide as the area of a circle, to show a relationship among the three.
There is a relationship between protein function, charge and hydrophobicity, and net charge and hydrophobicity can be used for describing the intermolecular force of protein. Some researches have found that protein hydrophobicity is significantly correlated with emulsification properties and solubility. Therefore, PepDraw is used for determining the chemical properties of polypeptide: isoelectric point, net charge and hydrophobicity.
The potential bioactive peptides in enzymolysis products of sturgeon roes are screened by mass spectrometry and virtual screening means and in combination with the indexes of peptide ranker, peak area and CPPpred prediction.
Results are shown in Table 5 and
It can be seen from Table 5 that 18 polypeptide sequences with potential biological activity are obtained from the enzymolysis products of sturgeon roes in this research. In combination with related data such as polypeptide activity score in
In this research, C57BL/6J mice are selected for experiments and divided into 8 groups: a normal group, a model group, a low-dosage sample group, a high-dosage sample group, two pure peptide sample groups and two control groups, with 5 mice in each group. Except the normal group, mice in the sample groups and the model group are injected with 5% D-galactose solution (125 mg/kg) subcutaneously at the neck and back daily at the beginning of the experiment to establish an aging model, which lasts for 8 weeks. The normal group and the control groups receive the same amount of saline injection. Starting from the 8th day of modeling, mice in the sample groups receive the corresponding intragastric sample treatment for 7 weeks. The control groups and the model group continuously receive constant normal saline intragastric administration with the same volume. During the experiment, all the mice are fed with an ordinary feed in an environment of 20-24° C. and operated according to specific dosage indexes.
The specific dosage information is shown in Table 6.
To evaluate oxidative stress-related indexes, after 1 h from final drug administration, the mice are subjected to intraperitoneal anesthesia with 3% pentobarbital sodium (30 mg/kg body weight), and then subjected to blood sampling through the orbital venous plexus of the mice. The obtained blood is stored in a standard blood sampling tube at room temperature for 1.5 h. Subsequently, the blood is centrifuged at 3000 rpm for 10 min and the supernatant is taken. According to the instructions of the kit, the supernatant is used for determining the activity of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA).
In addition, to determine the biochemical indexes related to the skin, a skin tissue sample is taken from the back hair removal area of the mice and the subcutaneous tissue below it is ensured to be excised, for sampling by about 0.1 g. The tissue sample is rinsed twice in pre-cooled normal saline and then drained for the surface moisture with filter paper. Next, the tissue is placed into a 4 mL EP tube and cut into smaller fragments. Under ice bath conditions, the pre-cooled normal saline (with a final tissue concentration of 0.1 g/mL) is added to these tissue fragments and the tissue fragments are homogenized. Then, the tissue fragments are centrifuged at 12000 rpm for 20 min to obtain the supernatants. Finally, the contents of collagen and hyaluronic acid (HA) are determined by using these supernatants according to the steps of the kit.
In this research, SD rats are used and divided into three groups: a normal group and sturgeon NLPL groups (50 mg/kg bw of daily dosage). At 12 h before the experiment, the rats are fasted but allowed to drink water. At 15 min, 45 min, 90 min, 180 min and 360 min after intragastric administration, all rats are anesthetized with 3% pentobarbital sodium, subjected to blood sampling through the abdominal aorta, and then euthanized. The polypeptide samples are prepared by solid phase synthesis and desalted.
The standard concentration is shown in Table 7.
Chromatographic separation is performed by using Waters ACQUITY UPLC I-CLASS ultra-high performance liquid chromatography equipment. Specific conditions are as follows: chromatographic column: Waters UPLC HSS T3 (1.8 μm, 2.1 mm×100 mm)-mobile phase: phase A (water, 0.1% formic acid), phase B (methanol)-flow rate: 0.3 mL/min-injection volume: 5.0 μL-column temperature: 40° C.-single sample analysis time: 8 min.
The gradient procedure of the mobile phase is shown in Table 8.
Mass spectrometric detection is performed by using Waters XEVO TQ-XS series four-pole mass spectrometer. Specific conditions are as follows: positive ion source voltage: 3.0 kV-cone well voltage: 30 V-solvent removal temperature: 500° C.-solvent removal gas flow rate: 1000 L/h-cone well gas flow rate: 150 L/h.
Data are expressed in the form of Mean±SD. Data processing and significance analysis are performed by Graphpad. P<0.05 and P<0.01 indicate significant difference and extremely significant difference in data. The target peak area in the polypeptide metabolism data is calculated by using TargetLynx software, and an allowable error of retention time is 15 s. The calculation of concentration is based on a standard curve method to obtain quantitative results.
Results are shown in
It can be seen from A in
It can be seen from B in
It can be seen from A in
It can be seen from B in
It can be seen from C in
It can be seen from D in
In conclusion, the enzymolysis products of sturgeon and sturgeon polypeptides have excellent effects on improving the oxidative stress level of aging mice.
Results are shown in
The corresponding standard curves are shown in
It can be seen from
The absorption of the sturgeon roe tetrapeptide NLPL in the body is shown in
It can be seen from
The above description of the disclosed embodiments enables those skilled in the art to realize or use the present invention. Many modifications made to these embodiments will be apparent to those skilled in the art. General principles defined herein can be realized in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202311694439.4 | Dec 2023 | CN | national |
