Patent Application
20230220088
The present invention relates to engineered antibodies that target human CCR5, as well as related engineered viruses. These antibodies and viruses are useful for addressing SARS-CoV-2 infection, HIV-1 infection, and other disorders.
Since the beginning of the AIDS epidemic, there has been an ongoing worldwide effort to develop effective anti-HIV-1 therapeutics and prophylactics. To date, this effort has yielded many failures and only a few successes. And, even the successful therapeutics and prophylactics are not without drawbacks such as serious side effects and the need for repeated administration. More recently, since the beginning of the COVID-19 outbreak, there has been—and continues to be—an intensive worldwide effort to develop effective anti-SARS-CoV-2 therapeutics and prophylactics. To date, this nascent effort has not succeeded.
For at least these reasons, there is an unmet need for a superior way to treat and prevent HIV-1 and SARS-CoV-2 infections, as well as other disorders addressable by targeting CCR5.
This invention provides a humanized monoclonal antibody having the light chain variable region amino acid sequence set forth in
This invention also provides a first humanized IgG4 monoclonal antibody having the light chain variable region amino acid sequence set forth in
This invention further provides a humanized monoclonal antibody having the light chain variable region amino acid sequence set forth in
This invention further provides a second humanized IgG4 monoclonal antibody having the light chain variable region amino acid sequence set forth in
This invention still further provides a humanized IgG2/IgG4 monoclonal fusion antibody having the light chain variable region amino acid sequence set forth in
This invention provides an isolated nucleic acid molecule encoding one or more chains of the present monoclonal antibody. This invention also provides a recombinant vector comprising the nucleotide sequence of the present nucleic acid molecule operably linked to a promoter of RNA transcription.
This invention also provides a composition comprising (i) the present monoclonal antibody, and (ii) a pharmaceutically acceptable carrier.
This invention further provides a recombinant AAV vector comprising a nucleic acid sequence encoding the heavy chain and/or the light chain of the present monoclonal antibody.
This invention still further provides a recombinant AAV particle comprising the present recombinant AAV vector and an AAV capsid protein, as well as a composition comprising (i) a plurality of the present AAV particles and (ii) a pharmaceutically acceptable carrier.
This invention provides a method for reducing the likelihood of a human subject's becoming infected with HIV-1 comprising administering to the subject a prophylactically effective amount of the present monoclonal antibody. This invention also provides a method for reducing the likelihood of a human subject's becoming infected with HIV-1 comprising administering to the subject a prophylactically effective number of the present recombinant AAV particles.
This invention provides a method for treating a human subject who is infected with HIV-1 comprising administering to the subject a therapeutically effective amount of the present monoclonal antibody. This invention also provides a method for treating a human subject who is infected with HIV-1 comprising administering to the subject a therapeutically effective number of the present recombinant AAV particles.
This invention provides a method for treating a human subject who is infected with SARS-CoV-2 comprising administering to the subject a therapeutically effective amount of the present monoclonal antibody. This invention also provides a method for treating a human subject who is infected with SARS-CoV-2 comprising administering to the subject a therapeutically effective number of the present recombinant AAV particles.
This invention provides a method for reducing the likelihood of a human subject's becoming afflicted with a CCR5-mediated disorder comprising administering to the subject a prophylactically effective amount of the present monoclonal antibody. This invention also provides a method for treating a human subject who is afflicted with a CCR5-mediated disorder comprising administering to the subject a therapeutically effective amount of the present monoclonal antibody. This invention further provides a method for reducing the likelihood of a human subject's becoming afflicted with a CCR5-mediated disorder comprising administering to the subject a prophylactically effective number of the present recombinant AAV particles. This invention still further provides a method for treating a human subject who is afflicted with a CCR5-mediated disorder comprising administering to the subject a therapeutically effective number of the present recombinant AAV particles. CCR5-mediated disorders include, without limitation, HIV-1 infection, hypercytokinemia, cytokine release syndrome, Alzheimer's disease, cancer (e.g., metastatic breast cancer), atherosclerosis, arthritis, inflammatory bowel disease, multiple sclerosis, and other immune-mediated illnesses such as graft-vs-host disease (GvHD) and non-alcoholic steatohepatitis (NASH).
This invention provides a first method for treating a human subject afflicted with a disorder comprising (i) administering to the subject a therapeutically effective amount of an agent for treating the disorder and (ii) administering to the subject a prophylactically effective amount of the present monoclonal antibody in conjunction with step (i), wherein the agent is known to cause cytokine release syndrome.
This invention also provides a second method for treating a human subject afflicted with a disorder comprising (i) administering to the subject a therapeutically effective amount of an agent for treating the disorder and (ii) administering to the subject a prophylactically effective number of the present AAV particles in conjunction with step (i), wherein the agent is known to cause cytokine release syndrome.
Finally, this invention provides three kits. The first kit comprises, in separate compartments, (a) a diluent and (b) a suspension of the present monoclonal antibody. The second kit comprises, in separate compartments, (a) a diluent and (b) the present monoclonal antibody in lyophilized form. The third kit comprises, in separate compartments, (a) a diluent and (b) a suspension of a plurality of the present recombinant AAV particles.
This figure shows a schematic diagram of an expression cassette encoding the present monoclonal antibody for inclusion in an AAV vector.
This invention provides certain recombinant monoclonal antibodies, recombinant viral vectors and particles, and related methods for addressing HIV-infection, SARS-CoV-2 infection, and other CCR5-mediated disorders like cancer.
In this application, certain terms are used which shall have the meanings set forth as follows.
As used herein, “administer”, with respect to monoclonal antibodies, means to deliver the antibodies to a subject's body via any known method suitable for that purpose. Specific modes of administration include, without limitation, intravenous, intramuscular, nasal, and subcutaneous administration. Preferably, the administration is subcutaneous. Similarly, as used herein, “administer”, with respect to recombinant viral particles, means to deliver the particles to a subject's body via any known method suitable for that purpose. Specific modes of administration include, without limitation, intravenous, intramuscular, and subcutaneous administration. Preferably, the administration is intravenous.
In this invention, monoclonal antibodies can be formulated using one or more routinely used pharmaceutically acceptable carriers. Such carriers are well known to those skilled in the art. For example, injectable drug delivery systems include solutions containing salts (e.g., sodium chloride and sodium phosphate). In a specific embodiment, the injectable drug delivery system comprises monoclonal antibody (e.g., 0.1 mg, 0.5 mg, 1 mg, 5 mg, 10 mg, 25 mg, 50 mg, 75 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, 225 mg, 250 mg, 275 mg, 300 mg, 325 mg, 350 mg, 375 mg, 400 mg, 425 mg, 450 mg, 475 mg, 500 mg, 525 mg, 550 mg, 575 mg, 600 mg, 625 mg, 650 mg, 675 mg, 700 mg, 725 mg, 750 mg, 775 mg, 800 mg, 825 mg, 850 mg, 875 mg, 900 mg, 925 mg, 950 mg, 975 mg, 1,000 mg, 1,250 mg, 1,500 mg, 1,750 mg, 2,000 mg, 2,250 mg, 2,500 mg, 2,750 mg, 3,000 mg, 3,250 mg, 3,500 mg, 3,750 mg, 4,000 mg, 4,250 mg, 4,500 mg, 4,750 mg, 5,000 mg, 5,250 mg, 5,500 mg, 5,750 mg, 6,000 mg, 6,250 mg, 6,500 mg, 6,750 mg, 7,000 mg, 7,250 mg, 7,500 mg, 7,750 mg, 8,000 mg, 8,250 mg, 8,500 mg, 8,750 mg, 9,000 mg, 9,250 mg, 9,500 mg, 9,750 mg, or 10,000 mg) in the form of a lyophilized powder in a multi-use vial, which is then reconstituted and diluted in, for example, 0.9% Sodium Chloride Injection, USP. In another specific embodiment, the injectable drug delivery system comprises monoclonal antibody (e.g., 0.1 mg/50 ml, 0.5 mg/50 ml, 1 mg/50 ml, 5 mg/50 ml, 10 mg/50 ml, 25 mg/50 ml, 50 mg/50 ml, 75 mg/50 ml, 100 mg/50 ml, 125 mg/50 ml, 150 mg/50 ml, 175 mg/50 ml, 200 mg/50 ml, 225 mg/50 ml, 250 mg/50 ml, 275 mg/50 ml, 300 mg/50 ml, 325 mg/50 ml, 350 mg/50 ml, 375 mg/50 ml, 400 mg/50 ml, 425 mg/50 ml, 450 mg/50 ml, 475 mg/50 ml, 500 mg/50 ml, 525 mg/50 ml, 550 mg/50 ml, 575 mg/50 ml, 600 mg/50 ml, 625 mg/50 ml, 650 mg/50 ml, 675 mg/50 ml, 700 mg/50 ml, 725 mg/50 ml, 750 mg/50 ml, 775 mg/50 ml, 800 mg/50 ml, 825 mg/50 ml, 850 mg/50 ml, 875 mg/50 ml, 900 mg/50 ml, 925 mg/50 ml, 950 mg/50 ml, 975 mg/50 ml, 1,000 mg/50 ml, 1,250 mg/50 ml, 1,500 mg/50 ml, 1,750 mg/50 ml, 2,000 mg/50 ml, 2,250 mg/50 ml, 2,500 mg/50 ml, 2,750 mg/50 ml, 3,000 mg/50 ml, 3,250 mg/50 ml, 3,500 mg/50 ml, 3,750 mg/50 ml, 4,000 mg/50 ml, 4,250 mg/50 ml, 4,500 mg/50 ml, 4,750 mg/50 ml, 5,000 mg/50 ml, 5,250 mg/50 ml, 5,500 mg/50 ml, 5,750 mg/50 ml, 6,000 mg/50 ml, 6,250 mg/50 ml, 6,500 mg/50 ml, 6,750 mg/50 ml, 7,000 mg/50 ml, 7,250 mg/50 ml, 7,500 mg/50 ml, 7,750 mg/50 ml, 8,000 mg/50 ml, 8,250 mg/50 ml, 8,500 mg/50 ml, 8,750 mg/50 ml, 9,000 mg/50 ml, 9,250 mg/50 ml, 9,500 mg/50 ml, 9,750 mg/50 ml, or 10,000 mg/50 ml) in the form of a suspension in a single-use vial, which is then withdrawn and diluted in, for example, 0.9% Sodium Chloride Injection, USP. Injectable drug delivery systems also include suspensions, gels, microspheres and polymeric injectables, and can comprise excipients such as solubility-altering agents (e.g., ethanol, propylene glycol, and sucrose) and polymers (e.g., polycaprylactones and PLGAs).
In addition, in this invention, recombinant viral particles can be formulated using one or more routinely used pharmaceutically acceptable carriers. Such carriers are well known to those skilled in the art. For example, injectable drug delivery systems include solutions containing salts (e.g., sodium chloride and sodium phosphate) and surfactants (e.g., a poloxamer). In a specific embodiment, the injectable drug delivery system comprises an aqueous solution of sodium chloride (e.g., 180 mM), sodium phosphate (e.g., 10 mM), and a poloxamer (e.g., 0.001% Poloxamer 188). Injectable drug delivery systems also include suspensions, gels, microspheres and polymeric injectables, and can comprise excipients such as solubility-altering agents (e.g., ethanol, propylene glycol, and sucrose) and polymers (e.g., polycaprylactones and PLGAs).
As used herein, the term “antibody” includes, without limitation, (a) an immunoglobulin molecule comprising two heavy chains (i.e., H chains, such as μ, δ, γ, α and ε) and two light chains (i.e., L chains, such as A and K) and which recognizes an antigen; (b) polyclonal and monoclonal immunoglobulin molecules; (c) monovalent and divalent fragments thereof, and (d) bispecific forms thereof. Immunoglobulin molecules may derive from any of the commonly known classes, including but not limited to IgA, secretory IgA, IgG and IgM. IgG subclasses are also well known to those in the art and include, but are not limited to, human IgG1, IgG2, IgG3 and IgG4 (preferably IgG2 and IgG4). Antibodies can be both naturally occurring and non-naturally occurring. Furthermore, antibodies include chimeric antibodies, wholly synthetic antibodies, single chain antibodies (e.g., scFv), and fragments thereof. Antibodies may contain, for example, all or a portion of a constant region (e.g., an Fc region) and a variable region, or contain only a variable region (responsible for antigen binding). Antibodies may be human, humanized, or nonhuman. Methods for designing and making humanized antibodies are well known (See, e.g., Chiu and Gilliland; Lafleur, et al.). Antibodies include, without limitation, the present monoclonal antibodies as defined herein.
“CCR5” (i.e., human CCR5), to which the present monoclonal antibody binds, is a member of the beta chemokine receptor family of integral membrane proteins. It is a G protein-coupled receptor (also known as a GPCR or seven-(pass)-transmembrane domain receptor) that functions as a chemokine receptor in the CC chemokine group. CCR5's ligands include CCL3 and CCL4 (also known as MIP 1α and 1β, respectively), CCL3L1, and CCR5 (also known as RANTES). These are small chemotactic cytokines that mediate chemo-attraction between cells.
As used herein, a “CCR5-mediated disorder” means a disorder wherein the presence of CCR5 in the afflicted subject plays a role in the disorder's formation, continuation, and/or progression. Examples of CCR5-mediated disorders include, without limitation, HIV-1 infection, hypercytokinemia, cytokine release syndrome, Alzheimer's disease, cancer (e.g., metastatic breast cancer), atherosclerosis, arthritis, inflammatory bowel disease, multiple sclerosis, and other immune-mediated illnesses such as graft-vs-host disease (GvHD) and non-alcoholic steatohepatitis (NASH). CCR5 plays a role in HIV-1 infection, in that it is a co-receptor for HIV-1 entry into, and infection of, target cells. PRO 140 binds tightly to CCR5 and is a powerful inhibitor of HIV-1 infection.
“Cytokine release syndrome” (CRS) is a potentially life-threatening systemic inflammatory response that follows the administration of certain therapeutic antibodies (e.g., Rituxan® (rituximab)) and adoptive T-cell therapies (e.g., Kymriah® (tisagenlecleucel)).
As used herein, “effector function”, with respect to an antibody, includes, without limitation, antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), and complement fixation. Methods for measuring effector function are known (see, e.g., R. P. Rother, et al.; C. Dumet, et al.; T. Schlothauer, et al.; C.-H. Lee, et al.; and O. Vafa, et al.). These known methods include, by way of example, the (i) surface-plasmon-resonance analysis; (ii) solution-phase complement-activation assay; (iii) enzyme-linked immunosorbent assay; (iv) C1q cell surface binding assay; (v) complement-dependent cytotoxicity (CDC) assays; (vi) complementor FcγR-mediated cellular cytotoxicity assays; (vii) complement-receptor inhibition assays; (viii) phagocytosis assays (ADCP or CDCP); (ix) anaphylaxis assay; and (x) FcγRIIb-mediated internalization assay, all as described in C.-H. Lee, et al. These methods also include, for example, the methods for determining (i) ADCC and CDC; (ii) ADCP; and (iii) Fc-dependent induction of cytokine release (FIC), all as described in O. Vafa, et al.
As used herein, a subject who has been “exposed” to HIV-1 includes, for example, a subject who experienced a high-risk event (e.g., one in which he/she came into contact with the bodily fluids of an infected human subject). In one embodiment, this exposure occurs one month, three weeks, two weeks, one week, five days, four days, three days, two days or 24 hours prior to receiving the present prophylaxis.
As used herein, a “human subject” can be of any age, gender or state of co-morbidity. In one embodiment, the subject is male, and in another, the subject is female. In another embodiment, the subject is co-morbid (e.g., afflicted with diabetes, asthma, and/or heart disease). In a further embodiment, the subject is not co-morbid. In still another embodiment, the subject is younger than 60 years old. In yet another embodiment, the subject is at least 60 years old, at least 65 years old, at least 70 years old, at least 75 years old, at least 80 years old, at least 85 years old, or at least 90 years old.
“Hypercytokinemia”, also known as a cytokine storm, is a severe immune reaction in which the body releases too many cytokines into the blood too quickly. In an afflicted subject, large numbers of white blood cells are activated and release inflammatory cytokines, which in turn activate yet more white blood cells. Hypercytokinemia can occur, for example, as a result of viral infection, as is the case with SARS-CoV-2, Ebola, and MERS-CoV. In SARS-CoV-2-infected subjects, pulmonary involvement leads to acute respiratory distress syndrome (ARDS), a life-threatening illness. ARDS results in shock, multi-organ failure including cardiac failure, and eventually death. For a subject infected with SARS-CoV-2, CCR5 blockade by the present monoclonal antibody can both inhibit the onset of, and reduce the severity of, hypercytokinemia.
As used herein, a heavy chain modification that “increases” the terminal half-life of the present monoclonal antibody is a modification that renders the present monoclonal antibody's terminal half-life longer than that of PRO 140 when administered at the same dose (e.g., 700 mg) and by the same route (e.g., subcutaneously, intravenously, or intramuscularly). In a preferred embodiment, the present monoclonal antibody's terminal half-life is longer than that of PRO 140 (when administered at the same dose and by the same route) by at least a factor of two, at least a factor of three, at least a factor of four, at least a factor of five, at least a factor of six, at least a factor of seven, at least a factor of eight, at least a factor of nine, at least a factor of 10, at least a factor of 15, at least a factor of 20, at least a factor of 25, at least a factor of 30, at least a factor of 35, at least a factor of 40, at least a factor of 45, or at least a factor of 50. In another preferred embodiment, the present monoclonal antibody's terminal half-life is at least two weeks, at least one month, at least two months, or at least three months. In a further preferred embodiment, the present monoclonal antibody's terminal half-life is at least five days, at least 10 days, at least 15 days, at least 20 days, at least 25 days, at least 30 days, at least 35 days, at least 40 days, at least 45 days, at least 50 days, at least 55 days, at least 60 days, at least 65 days, at least 70 days, at least 75 days, at least 80 days, at least 85 days, at least 90 days, at least 95 days, or at least 100 days. In a further preferred embodiment, the present monoclonal antibody's terminal half-life is from five days to 10 days, from 10 days to 15 days, from 15 days to 20 days, from 20 days to 25 days, from 25 days to 30 days, from 30 days to 35 days, from 35 days to 40 days, from 40 days to 45 days, from 45 days to 50 days, from 50 days to 55 days, from 55 days to 60 days, from 60 days to 65 days, from 65 days to 70 days, from 70 days to 75 days, from 75 days to 80 days, from 80 days to 85 days, from 85 days to 90 days, from 90 days to 95 days, from 95 days to 100 days, or over 100 days. An antibody's terminal half-life (e.g., its mean terminal half-life) can be determined, for example, based on data from in vivo human studies, and can also be determined, for example, based on data from animal studies (e.g., studies in mice (e.g., human FcRn transgenic mice available from Jackson Labs (see, e.g., G. Proetzel and D. C. Roopenian; Avery, et al.; and D. Sheridan, et al.)), rats, rabbits, and monkeys (such as rhesus monkeys, cynamolgous macaques, and marmosets)). Methods for determining an antibody's terminal half-life are known (see, e.g., P. L. Toutain and A. Bousquet-Melou). Examples of heavy chain modifications that increase antibody terminal half-life (such as those that increase antibody binding to FcRn) are described in C. Dumet, et al. and G. J. Robbie, et al. They include, without limitation, the following, with numbering according to the EU Index: (i) point mutations at position 252, 254, 256, 309, 311, 433, 434, and/or 436, including the “YTE” mutation combination M252Y/S254T/T256E (U.S. Pat. No. 7,083,784); (ii) the “LS” mutation combination M428L/N434S (WO/2009/086320); (iii) the “QL” mutation combination T250Q/M428L; and (iv) the mutation combinations M428L/V308F and Q311V/N434S. By way of example, the heavy chain modification M252Y/S254T/T256E (YTE) increases the terminal half-life of the present monoclonal antibody if the present monoclonal antibody's terminal half-life is 10 days and that of PRO 140 is three days, when the present monoclonal antibody and PRO 140 are both administered subcutaneously at a 700 mg dose.
As used herein, a subject is “infected” with a virus if the virus is present in the subject. Present in the subject includes, without limitation, present in at least some cells in the subject, and/or present in at least some extracellular fluid in the subject. In one embodiment, the virus present in the subject's cells is replicating. A subject who is exposed to a virus may or may not become infected with it.
Heavy chain modifications that “inhibit half antibody formation” in IgG4 are described, for example, in C. Dumet, et al. They include, without limitation, the following, with numbering according to the EU Index: (i) S228P; (ii) the mutation combination S228P/R409K, and (iii) the mutation combination S228P/K447del. Moreover, the mutations 447del and 446/447del are known to reduce IgG4 C-terminal heterogeneity, as described in C. Dumet.
As used herein, a heavy chain modification that “lowers the effector function” of the present monoclonal antibody is a modification that renders the present monoclonal antibody's effector function lower than that of PRO 140. These effector function-lowering heavy chain modifications include, without limitation, IgG4 heavy chain point mutations and deletion mutations. Examples of IgG4 heavy chain modifications that lower effector function relative to wild-type IgG4 heavy chains are described in C. Dumet, et al. They include, without limitation, the following, with numbering according to the EU Index: (i) L235E (WO/1994/028027); (ii) L235A, F234A, and G237A (WO/1994/029351 and WO/1995/026403); (iii) D265A (U.S. Pat. No. 7,332,581); (iv) L328 substitution, A330R, and F243L (WO/2004/029207); (v) IgG2/IgG4 format wherein IgG2 (up to T260) is joined to IgG4 (WO/2005/007809); (vi) F243A/V264A combination (WO/2011/149999); (vii) E233P/F234A/L235A/G236del/G237A combination (WO/2017/079369); and (viii) S228P/L235E combination.
“PRO 140 #1” and “PRO 140 #2”, as defined in U.S. Pat. No. 7,122,185, are referred to herein, collectively and individually, as “PRO 140.” PRO 140 #1 has the heavy and light chain variable region amino acid sequences set forth in
As used herein, a “prophylactically effective amount” of the present monoclonal antibodies includes, without limitation, (i) 0.1 mg, 0.5 mg, 1 mg, 5 mg, 10 mg, 15 mg, 20 mg, 25 mg, 30 mg, 35 mg, 40 mg, 45 mg, 50 mg, 60 mg, 70 mg, 80 mg, 90 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, 225 mg, 250 mg, 275 mg, 300 mg, 325 mg, 350 mg, 375 mg, 400 mg, 425 mg, 450 mg, 475 mg, 500 mg, 525 mg, 550 mg, 575 mg, 600 mg, 625 mg, 650 mg, 675 mg, 700 mg, 725 mg, 750 mg, 775 mg, 800 mg, 825 mg, 850 mg, 875 mg, 900 mg, 925 mg, 950 mg, 975 mg, 1,000 mg, 1,250 mg, 1,500 mg, 1,750 mg, 2,000 mg, 2,250 mg, 2,500 mg, 2,750 mg, 3,000 mg, 3,250 mg, 3,500 mg, 3,750 mg, 4,000 mg, 4,250 mg, 4,500 mg, 4,750 mg, 5,000 mg, 5,250 mg, 5,500 mg, 5,750 mg, 6,000 mg, 6,250 mg, 6,500 mg, 6,750 mg, 7,000 mg, 7,250 mg, 7,500 mg, 7,750 mg, 8,000 mg, 8,250 mg, 8,500 mg, 8,750 mg, 9,000 mg, 9,250 mg, 9,500 mg, 9,750 mg, or 10,000 mg; (ii) 0.1 mg to 1 mg, 1 mg to 5 mg, 5 mg to 20 mg, 20 mg to 50 mg, 50 mg to 100 mg, 100 mg to 150 mg, 150 mg to 200 mg, 200 mg to 250 mg, 250 mg to 300 mg, 300 mg to 350 mg, 350 mg to 400 mg, 400 mg to 450 mg, 450 mg to 500 mg, 500 mg to 550 mg, 550 mg to 600 mg, 600 mg to 650 mg, 650 mg to 700 mg, 700 mg to 750 mg, 750 mg to 800 mg, 800 mg to 850 mg, 850 mg to 900 mg, 900 mg to 950 mg, 950 mg to 1,000 mg, 1,000 mg to 1,250 mg, 1,250 mg to 1,500 mg, 1,500 mg to 1,750 mg, 1,750 mg to 2,000 mg, 2,000 mg to 2,250 mg, 2,250 mg to 2,500 mg, 2,500 mg to 2,750 mg, 2,750 mg to 3,000 mg, 3,000 mg to 3,250 mg, 3,250 mg to 3,500 mg, 3,500 mg to 3,750 mg, 3,750 mg to 4,000 mg, 4,000 mg to 4,250 mg, 4,250 mg to 4,500 mg, 4,500 mg to 4,750 mg, 4,750 mg to 5,000 mg, 5,000 mg to 5,250 mg, 5,250 mg to 5,500 mg, 5,500 mg to 5,750 mg, 5,750 mg to 6,000 mg, 6,000 mg to 6,250 mg, 6,250 mg to 6,500 mg, 6,500 mg to 6,750 mg, 6,750 mg to 7,000 mg, 7,000 mg to 7,250 mg, 7,250 mg to 7,500 mg, 7,500 mg to 7,750 mg, 7,750 mg to 8,000 mg, 8,000 mg to 8,250 mg, 8,250 mg to 8,500 mg, 8,500 mg to 8,750 mg, 8,750 mg to 9,000 mg, 9,000 mg to 9,250 mg, 9,250 mg to 9,500 mg, 9,500 mg to 9,750 mg, or 9,750 mg to 10,000 mg; (iii) 0.1 mg/kg, 0.2 mg/kg, 0.3 mg/kg, 0.4 mg/kg, 0.5 mg/kg, 0.6 mg/kg, 0.7 mg/kg, 0.8 mg/kg, 0.9 mg/kg, 1 mg/kg, 2 mg/kg, 3 mg/kg, 4 mg/kg, 5 mg/kg, 6 mg/kg, 7 mg/kg, 8 mg/kg, 9 mg/kg, 10 mg/kg, 15 mg/kg, 20 mg/kg, 25 mg/kg, 30 mg/kg, 35 mg/kg, 40 mg/kg, 45 mg/kg, 50 mg/kg, 60 mg/kg, 70 mg/kg, 80 mg/kg, 90 mg/kg, 100 mg/kg, 200 mg/kg, 300 mg/kg, 400 mg/kg, or 500 mg/kg; or (iv) 0.1 mg/kg to 1 mg/kg, 1 mg/kg to 10 mg/kg, 10 mg/kg to 20 mg/kg, 20 mg/kg to 30 mg/kg, 30 mg/kg to 40 mg/kg, 40 mg/kg to 50 mg/kg, 50 mg/kg to 60 mg/kg, 60 mg/kg to 70 mg/kg, 70 mg/kg to 80 mg/kg, 80 mg/kg to 90 mg/kg, 90 mg/kg to 100 mg/kg, 100 mg/kg to 200 mg/kg, 200 mg/kg to 300 mg/kg, 300 mg/kg to 400 mg/kg, or 400 mg/kg to 500 mg/kg. In the preferred embodiment, the prophylactically effective amount of monoclonal antibodies is 175 mg, 350 mg, or 700 mg, administered subcutaneously. In another preferred embodiment, the prophylactically effective amount of monoclonal antibodies is administered as a single, one-time-only dose. In a further embodiment, the prophylactically effective amount of monoclonal antibodies is administered as two or more doses over a period of days, weeks, or months (e.g., twice daily for one or two weeks; once daily for one or two weeks; every other day for two weeks; three times per week for two weeks; twice per week for two weeks; once per week for two weeks; twice with the administrations separated by two weeks; once per month; once every two months; once every three months; once every four months; twice per year; or once per year).
In a preferred embodiment, the prophylactically effective amount of the present monoclonal antibodies is administered according to a regimen for pre-exposure prophylaxis (“PrEP”) for the prevention of HIV infection. PrEP involves the regular (e.g., daily, weekly, monthly, semiannual, or annual) use of anti-retroviral therapy before and after events involving the risk of HIV exposure, in order to lower the chances of acquiring HIV infection. These events include sex and intravenous drug use, for example. The CDC provides a clinical practice guideline for PrEP (“Preexposure Prophylaxis for the Prevention of HIV Infection in the United States—2021 Update”), and PrEP is also the subject of ongoing research (see, e.g., J. Riddell IV, et al.; H. Yusuf, et al.; and R. Chou, et al.). In addition, preferred embodiments of PrEP for the present monoclonal antibodies are set forth in the Examples section below.
As used herein, a “prophylactically effective amount” of the present recombinant viral particles (e.g., recombinant AAV particles) includes, without limitation, (i) from 1×1010 to 5×1010 particles (also referred to as “viral genomes” or “vg”) per kg of body weight, from 5×1010 to 1×1011 particles/kg, from 1×1011 to 5×1011 particles/kg, from 5×1011 to 1×1012 particles/kg, from 1×1012 to 5×1012 particles/kg, from 5×1012 to 1×1013 particles/kg, from 1×1013 to 5×1013 particles/kg, or from 5×1013 to 1×1014 particles/kg; or (ii) 1×1010 particles/kg, 5×1010 particles/kg, 1×1011 particles/kg, 5×1011 particles/kg, 1×1012 particles/kg, 5×1012 particles/kg, 1×1013 particles/kg, 5×1013 particles/kg, or 1×1014 particles/kg, 5×1014 particles/kg, or 1×1015 particles/kg. In the preferred embodiment, the prophylactically effective amount of viral particles is administered as a single, one-time-only dose. In another embodiment, the prophylactically effective amount of viral particles is administered as two or more doses over a period of months or years.
As used herein, a “recombinant AAV (adeno-associated virus) particle”, also referred to as “rAAV particle”, includes, without limitation, an AAV capsid protein (e.g., VP1, VP2 and/or VP3) and a vector comprising a nucleic acid encoding an exogenous protein (e.g., an antibody heavy chain) situated between a pair of AAV inverted terminal repeats in a manner permitting the AAV particle to infect a target cell. Preferably, the recombinant AAV particle is incapable of replication within its target cell. The AAV serotype may be any AAV serotype suitable for use in gene therapy, such as AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAVrh10, AAV11, AAV12, LK01, LK02 or LK03.
As used herein, “reducing the likelihood” of a human subject's becoming infected with a virus includes, without limitation, reducing such likelihood by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 99%. Preferably, reducing the likelihood of a human subject's becoming infected with a virus means preventing the subject from becoming infected with it. Similarly, “reducing the likelihood” of a human subject's becoming symptomatic of a viral infection includes, without limitation, reducing such likelihood by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 99%. Preferably, reducing the likelihood of a human subject's becoming symptomatic of a viral infection means preventing the subject from becoming symptomatic.
As used herein, the term “subject” includes, without limitation, a mammal such as a human, a non-human primate, a dog, a cat, a horse, a sheep, a goat, a cow, a rabbit, a pig, a hamster, a rat and a mouse. The present methods are envisioned for these non-human embodiments, mutatis mutandis, as they are for human subjects in this invention.
As used herein, a human subject is “symptomatic” of an HIV-1 infection if the subject shows one or more symptoms known to appear in an HIV-1-infected human subject after a suitable incubation period. Such symptoms include, without limitation, detectable HIV-1 in the subject, and those symptoms shown by patients afflicted with AIDS. AIDS-related symptoms include, without limitation, weight loss, fever, fatigue and opportunistic infections.
As used herein, a human subject is “symptomatic” of a SARS-CoV-2 infection if the subject shows one or more symptoms known to appear in a SARS-CoV-2-infected human subject after a suitable incubation period. Such symptoms include, without limitation, detectable SARS-CoV-2 in the subject, and those symptoms shown by patients afflicted with COVID-19. COVID-19-related symptoms include, without limitation, fever, cough, shortness of breath, persistent pain or pressure in the chest, new confusion or inability to arouse, and/or bluish lips or face.
As used herein, a “therapeutically effective amount” of the present monoclonal antibodies includes, without limitation, (i) 0.1 mg, 0.5 mg, 1 mg, 5 mg, 10 mg, 15 mg, 20 mg, 25 mg, 30 mg, 35 mg, 40 mg, 45 mg, 50 mg, 60 mg, 70 mg, 80 mg, 90 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, 225 mg, 250 mg, 275 mg, 300 mg, 325 mg, 350 mg, 375 mg, 400 mg, 425 mg, 450 mg, 475 mg, 500 mg, 525 mg, 550 mg, 575 mg, 600 mg, 625 mg, 650 mg, 675 mg, 700 mg, 725 mg, 750 mg, 775 mg, 800 mg, 825 mg, 850 mg, 875 mg, 900 mg, 925 mg, 950 mg, 975 mg, 1,000 mg, 1,250 mg, 1,500 mg, 1,750 mg, 2,000 mg, 2,250 mg, 2,500 mg, 2,750 mg, 3,000 mg, 3,250 mg, 3,500 mg, 3,750 mg, 4,000 mg, 4,250 mg, 4,500 mg, 4,750 mg, 5,000 mg, 5,250 mg, 5,500 mg, 5,750 mg, 6,000 mg, 6,250 mg, 6,500 mg, 6,750 mg, 7,000 mg, 7,250 mg, 7,500 mg, 7,750 mg, 8,000 mg, 8,250 mg, 8,500 mg, 8,750 mg, 9,000 mg, 9,250 mg, 9,500 mg, 9,750 mg, or 10,000 mg; (ii) 0.1 mg to 1 mg, 1 mg to 5 mg, 5 mg to 20 mg, 20 mg to 50 mg, 50 mg to 100 mg, 100 mg to 150 mg, 150 mg to 200 mg, 200 mg to 250 mg, 250 mg to 300 mg, 300 mg to 350 mg, 350 mg to 400 mg, 400 mg to 450 mg, 450 mg to 500 mg, 500 mg to 550 mg, 550 mg to 600 mg, 600 mg to 650 mg, 650 mg to 700 mg, 700 mg to 750 mg, 750 mg to 800 mg, 800 mg to 850 mg, 850 mg to 900 mg, 900 mg to 950 mg, 950 mg to 1,000 mg, 1,000 mg to 1,250 mg, 1,250 mg to 1,500 mg, 1,500 mg to 1,750 mg, 1,750 mg to 2,000 mg, 2,000 mg to 2,250 mg, 2,250 mg to 2,500 mg, 2,500 mg to 2,750 mg, 2,750 mg to 3,000 mg, 3,000 mg to 3,250 mg, 3,250 mg to 3,500 mg, 3,500 mg to 3,750 mg, 3,750 mg to 4,000 mg, 4,000 mg to 4,250 mg, 4,250 mg to 4,500 mg, 4,500 mg to 4,750 mg, 4,750 mg to 5,000 mg, 5,000 mg to 5,250 mg, 5,250 mg to 5,500 mg, 5,500 mg to 5,750 mg, 5,750 mg to 6,000 mg, 6,000 mg to 6,250 mg, 6,250 mg to 6,500 mg, 6,500 mg to 6,750 mg, 6,750 mg to 7,000 mg, 7,000 mg to 7,250 mg, 7,250 mg to 7,500 mg, 7,500 mg to 7,750 mg, 7,750 mg to 8,000 mg, 8,000 mg to 8,250 mg, 8,250 mg to 8,500 mg, 8,500 mg to 8,750 mg, 8,750 mg to 9,000 mg, 9,000 mg to 9,250 mg, 9,250 mg to 9,500 mg, 9,500 mg to 9,750 mg, or 9,750 mg to 10,000 mg; (iii) 0.1 mg/kg, 0.2 mg/kg, 0.3 mg/kg, 0.4 mg/kg, 0.5 mg/kg, 0.6 mg/kg, 0.7 mg/kg, 0.8 mg/kg, 0.9 mg/kg, 1 mg/kg, 2 mg/kg, 3 mg/kg, 4 mg/kg, 5 mg/kg, 6 mg/kg, 7 mg/kg, 8 mg/kg, 9 mg/kg, 10 mg/kg, 15 mg/kg, 20 mg/kg, 25 mg/kg, 30 mg/kg, 35 mg/kg, 40 mg/kg, 45 mg/kg, 50 mg/kg, 60 mg/kg, 70 mg/kg, 80 mg/kg, 90 mg/kg, 100 mg/kg, 200 mg/kg, 300 mg/kg, 400 mg/kg, or 500 mg/kg; or (iv) 0.1 mg/kg to 1 mg/kg, 1 mg/kg to 10 mg/kg, 10 mg/kg to 20 mg/kg, 20 mg/kg to 30 mg/kg, 30 mg/kg to 40 mg/kg, 40 mg/kg to 50 mg/kg, 50 mg/kg to 60 mg/kg, 60 mg/kg to 70 mg/kg, 70 mg/kg to 80 mg/kg, 80 mg/kg to 90 mg/kg, 90 mg/kg to 100 mg/kg, 100 mg/kg to 200 mg/kg, 200 mg/kg to 300 mg/kg, 300 mg/kg to 400 mg/kg, or 400 mg/kg to 500 mg/kg. In the preferred embodiment, the therapeutically effective amount of monoclonal antibodies is 175 mg, 350 mg, or 700 mg, administered subcutaneously. In another preferred embodiment, the therapeutically effective amount of monoclonal antibodies is administered as a single, one-time-only dose. In a further embodiment, the therapeutically effective amount of monoclonal antibodies is administered as two or more doses over a period of days, weeks, or months (e.g., twice daily for one or two weeks; once daily for one or two weeks; every other day for two weeks; three times per week for two weeks; twice per week for two weeks; once per week for two weeks; twice with the administrations separated by two weeks; once per month; once every two months; once every three months; once every four months; twice per year; or once per year).
As used herein, a “therapeutically effective amount” of the present recombinant viral particles (e.g., recombinant AAV particles) includes, without limitation, (i) from 1×1010 to 5×1010 particles (also referred to as “viral genomes” or “vg”) per kg of body weight, from 5×1010 to 1×1011 particles/kg, from 1×1011 to 5×1011 particles/kg, from 5×1011 to 1×1012 particles/kg, from 1×1012 to 5×1012 particles/kg, from 5×1012 to 1×1013 particles/kg, from 1×1013 to 5×1013 particles/kg, or from 5×1013 to 1×1014 particles/kg; or (ii) 1×1010 particles/kg, 5×1010 particles/kg, 1×1011 particles/kg, 5×1011 particles/kg, 1×1012 particles/kg, 5×1012 particles/kg, 1×1013 particles/kg, 5×1013 particles/kg, or 1×1014 particles/kg, 5×1014 particles/kg, or 1×1015 particles/kg. In the preferred embodiment, the therapeutically effective amount of viral particles is administered as a single, one-time-only dose. In another embodiment, the therapeutically effective amount of viral particles is administered as two or more doses over a period of months or years.
As used herein, “treating” a subject afflicted with a disorder (e.g., infected with SARS-CoV-2 and symptomatic of that infection; infected with HIV-1 and symptomatic of that infection; or afflicted with another CCR5-mediated disorder) includes, without limitation, (i) slowing, stopping, or reversing the progression of one or more of the disorder's symptoms, (ii) slowing, stopping or reversing the progression of the disorder underlying such symptoms, (iii) reducing or eliminating the likelihood of the symptoms' recurrence, and/or (iv) slowing the progression of, lowering or eliminating the disorder. In the preferred embodiment, treating a subject afflicted with a disorder includes (i) reversing the progression of one or more of the disorder's symptoms, (ii) reversing the progression of the disorder underlying such symptoms, (iii) preventing the symptoms' recurrence, and/or (iv) eliminating the disorder. For a subject infected with SARS-CoV-2 but not symptomatic of that infection, “treating” the subject also includes, without limitation, reducing the likelihood of the subject's becoming symptomatic of the infection, and preferably, preventing the subject from becoming symptomatic of the infection. When the disorder is cancer, treating a subject includes, without limitation, slowing, stopping, or reversing metastasis.
This invention provides certain anti-CCR5 monoclonal antibodies. These antibodies possess the light and heavy chain variable region amino acid sequences of PRO 140 (leronlimab), and differ from PRO 140 with respect to certain heavy chain modifications that inhibit half antibody formation, increase terminal half-life, and lower the effector function. It also provides recombinant viral particles (preferably recombinant AAV particles) that, when introduced into a subject, cause the long-term expression of those antibodies. These antibodies and viral particles address SARS-CoV-2 infection, HIV-1 infection, and CCR5-related diseases.
Specifically, this invention provides a humanized monoclonal antibody having the light chain variable region amino acid sequence set forth in
This invention also provides a first humanized IgG4 monoclonal antibody having the light chain variable region amino acid sequence set forth in
In one embodiment, the first humanized IgG4 (preferably IgG4κ) monoclonal antibody has the light chain variable region amino acid sequence set forth in
In a preferred embodiment of the first humanized IgG4 (preferably IgG4κ) monoclonal antibody, the half antibody formation-inhibiting mutation is selected from the group consisting of S228P, the S228P/R409K combination, and the S228P/K447del combination (with numbering according to the EU Index).
In another preferred embodiment of the first humanized IgG4 (preferably IgG4κ) monoclonal antibody, the antibody comprises a terminal half-life-extending mutation combination selected from the group consisting of M252Y/S254T/T256E (YTE) and M428L/N434S (LS) (with numbering according to the EU Index).
This invention provides a humanized monoclonal antibody having the light chain variable region amino acid sequence set forth in
This invention also provides a second humanized IgG4 monoclonal antibody having the light chain variable region amino acid sequence set forth in
In one embodiment, the second humanized IgG4 (preferably IgG4κ) monoclonal antibody has the light chain variable region amino acid sequence set forth in
In a preferred embodiment of the second humanized IgG4 (preferably IgG4κ) monoclonal antibody, the half antibody formation-inhibiting mutation is selected from the group consisting of S228P, the S228P/R409K combination, and the S228P/K447del combination (with numbering according to the EU Index).
In another preferred embodiment of the second humanized IgG4 (preferably IgG4κ) monoclonal antibody, the antibody comprises a terminal half-life-extending mutation combination selected from the group consisting of M252Y/S254T/T256E (YTE) and M428L/N434S (LS) (with numbering according to the EU Index).
In a further preferred embodiment of the second humanized IgG4 (preferably IgG4κ) monoclonal antibody, the antibody comprises an effector function-lowering mutation selected from the group consisting of L235E, L235A, F234A, G237A, D265A, an L328 substitution, A330R, F243L, the F243A/V264A combination, the E233P/F234A/L235A/G236del/G237A combination, and the S228P/L235E combination (with numbering according to the EU Index).
This invention further provides a humanized IgG2/IgG4 monoclonal fusion antibody having the light chain variable region amino acid sequence set forth in
In one embodiment, the humanized IgG2/IgG4 (preferably IgG2/IgG4κ) monoclonal fusion antibody has the light chain variable region amino acid sequence set forth in
In a preferred embodiment of the humanized IgG2/IgG4 (preferably IgG2/IgG4κ) monoclonal fusion antibody, the antibody comprises a terminal half-life-extending mutation combination selected from the group consisting of M252Y/S254T/T256E (YTE) and M428L/N434S (LS) (with numbering according to the EU Index).
The above monoclonal antibodies are referred to herein, collectively and individually, as the present monoclonal antibody.
The following exemplary antibodies are envisioned as embodiments of the present monoclonal antibody. The first exemplary antibody is a humanized IgG4 (preferably IgG4κ) monoclonal antibody that (i) has the terminal half-life-extending mutation combination M252Y/S254T/T256E (YTE); and (ii) has the half antibody formation-inhibiting mutation S228P (all with numbering according to the EU Index). In one embodiment, the first exemplary antibody has the effector function-lowering L235E mutation (with numbering according to the EU Index). In another embodiment, the first exemplary antibody has one or more of the effector function-lowering mutations L235A, F234A, and G237A (with numbering according to the EU Index). In a further embodiment, the first exemplary antibody has the effector function-lowering D265A mutation (with numbering according to the EU Index). In a further embodiment, the first exemplary antibody has one or more of the effector function-lowering mutations A330R, F243L, and an L328 substitution (with numbering according to the EU Index). In a further embodiment, the first exemplary antibody has the effector function-lowering F243A/V264A mutation combination (with numbering according to the EU Index). In a further embodiment, the first exemplary antibody has the effector function-lowering E233P/F234A/L235A/G236del/G237A mutation combination (with numbering according to the EU Index). In yet a further embodiment, the first exemplary antibody has the effector function-lowering S228P/L235E mutation combination (with numbering according to the EU Index).
The second exemplary antibody is a humanized IgG4 (preferably IgG4κ) monoclonal antibody that (i) has the terminal half-life-extending mutation combination M252Y/S254T/T256E (YTE); and (ii) has the half antibody formation-inhibiting mutation combination S228P/R409K (all with numbering according to the EU Index). In one embodiment, the second exemplary antibody has the effector function-lowering L235E mutation (with numbering according to the EU Index). In another embodiment, the second exemplary antibody has one or more of the effector function-lowering mutations L235A, F234A, and G237A (with numbering according to the EU Index). In a further embodiment, the second exemplary antibody has the effector function-lowering D265A mutation (with numbering according to the EU Index). In a further embodiment, the second exemplary antibody has one or more of the effector function-lowering mutations A330R, F243L, and an L328 substitution (with numbering according to the EU Index). In a further embodiment, the second exemplary antibody has the effector function-lowering F243A/V264A mutation combination (with numbering according to the EU Index). In a further embodiment, the second exemplary antibody has the effector function-lowering E233P/F234A/L235A/G236del/G237A mutation combination (with numbering according to the EU Index). In yet a further embodiment, the second exemplary antibody has the effector function-lowering S228P/L235E mutation combination (with numbering according to the EU Index).
The third exemplary antibody is a humanized IgG4 (preferably IgG4κ) monoclonal antibody that (i) has the terminal half-life-extending mutation combination M252Y/S254T/T256E (YTE); and (ii) has the half antibody formation-inhibiting mutation combination S228P/K447del (all with numbering according to the EU Index). In one embodiment, the third exemplary antibody has the effector function-lowering L235E mutation (with numbering according to the EU Index). In another embodiment, the third exemplary antibody has one or more of the effector function-lowering mutations L235A, F234A, and G237A (with numbering according to the EU Index). In a further embodiment, the third exemplary antibody has the effector function-lowering D265A mutation (with numbering according to the EU Index). In a further embodiment, the third exemplary antibody has one or more of the effector function-lowering mutations A330R, F243L, and an L328 substitution (with numbering according to the EU Index). In a further embodiment, the third exemplary antibody has the effector function-lowering F243A/V264A mutation combination (with numbering according to the EU Index). In a further embodiment, the third exemplary antibody has the effector function-lowering E233P/F234A/L235A/G236del/G237A mutation combination (with numbering according to the EU Index). In yet a further embodiment, the third exemplary antibody has the effector function-lowering S228P/L235E mutation combination (with numbering according to the EU Index).
The fourth exemplary antibody is a humanized IgG4 (preferably IgG4κ) monoclonal antibody that (i) has the terminal half-life-extending mutation combination M428L/N434S (LS); and (ii) has the half antibody formation-inhibiting mutation S228P (all with numbering according to the EU Index). In one embodiment, the fourth exemplary antibody has the effector function-lowering L235E mutation (with numbering according to the EU Index). In another embodiment, the fourth exemplary antibody has one or more of the effector function-lowering mutations L235A, F234A, and G237A (with numbering according to the EU Index). In a further embodiment, the fourth exemplary antibody has the effector function-lowering D265A mutation (with numbering according to the EU Index). In a further embodiment, the fourth exemplary antibody has one or more of the effector function-lowering mutations A330R, F243L, and an L328 substitution (with numbering according to the EU Index). In a further embodiment, the fourth exemplary antibody has the effector function-lowering F243A/V264A mutation combination (with numbering according to the EU Index). In a further embodiment, the fourth exemplary antibody has the effector function-lowering E233P/F234A/L235A/G236del/G237A mutation combination (with numbering according to the EU Index). In yet a further embodiment, the fourth exemplary antibody has the effector function-lowering S228P/L235E mutation combination (with numbering according to the EU Index).
The fifth exemplary antibody is a humanized IgG4 (preferably IgG4κ) monoclonal antibody that (i) has the terminal half-life-extending mutation combination M428L/N434S (LS); and (ii) has the half antibody formation-inhibiting mutation combination S228P/R409K (all with numbering according to the EU Index). In one embodiment, the fifth exemplary antibody has the effector function-lowering L235E mutation (with numbering according to the EU Index). In another embodiment, the fifth exemplary antibody has one or more of the effector function-lowering mutations L235A, F234A, and G237A (with numbering according to the EU Index). In a further embodiment, the fifth exemplary antibody has the effector function-lowering D265A mutation (with numbering according to the EU Index). In a further embodiment, the fifth exemplary antibody has one or more of the effector function-lowering mutations A330R, F243L, and an L328 substitution (with numbering according to the EU Index). In a further embodiment, the fifth exemplary antibody has the effector function-lowering F243A/V264A mutation combination (with numbering according to the EU Index). In a further embodiment, the fifth exemplary antibody has the effector function-lowering E233P/F234A/L235A/G236del/G237A mutation combination (with numbering according to the EU Index). In yet a further embodiment, the fifth exemplary antibody has the effector function-lowering S228P/L235E mutation combination (with numbering according to the EU Index).
The sixth exemplary antibody is a humanized IgG4 (preferably IgG4κ) monoclonal antibody that (i) has the terminal half-life-extending mutation combination M428L/N434S (LS); and (ii) has the half antibody formation-inhibiting mutation combination S228P/K447del (all with numbering according to the EU Index). In one embodiment, the sixth exemplary antibody has the effector function-lowering L235E mutation (with numbering according to the EU Index). In another embodiment, the sixth exemplary antibody has one or more of the effector function-lowering mutations L235A, F234A, and G237A (with numbering according to the EU Index). In a further embodiment, the sixth exemplary antibody has the effector function-lowering D265A mutation (with numbering according to the EU Index). In a further embodiment, the sixth exemplary antibody has one or more of the effector function-lowering mutations A330R, F243L, and an L328 substitution (with numbering according to the EU Index). In a further embodiment, the sixth exemplary antibody has the effector function-lowering F243A/V264A mutation combination (with numbering according to the EU Index). In a further embodiment, the sixth exemplary antibody has the effector function-lowering E233P/F234A/L235A/G236del/G237A mutation corn bination (with numbering according to the EU Index). In yet a further embodiment, the sixth exemplary antibody has the effector function-lowering S228P/L235E mutation combination (with numbering according to the EU Index).
The seventh exemplary antibody is a humanized IgG2/IgG4 (preferably IgG2/IgG4κ) monoclonal fusion antibody that has the terminal half-life-extending mutation combination M428L/N434S (LS) (with numbering according to the EU Index).
The eighth exemplary antibody is a humanized IgG2/IgG4 (preferably IgG2/IgG4κ) monoclonal fusion antibody that has the terminal half-life-extending mutation combination M252Y/S254T/T256E (YTE) (with numbering according to the EU Index).
This invention provides an isolated nucleic acid molecule encoding one or more chains of the present monoclonal antibody. In one embodiment, the present nucleic acid molecule is a DNA molecule, for example, a cDNA molecule.
This invention further provides a recombinant vector, for example a plasmid or a viral vector, comprising the present nucleic acid molecule operably linked to a promoter of RNA transcription.
This invention still further provides a host vector system comprising one or more of the present vectors in a suitable host cell (e.g., a bacterial cell, an insect cell, a yeast cell, or a mammalian cell such as a hybridoma cell (See, e.g., Chiu and Gilliland; Kohler and Milstein)). This invention further provides a method for producing the present monoclonal antibody comprising culturing the present host vector system.
In connection with the present vectors, a nucleic acid sequence “encoding” a protein (e.g., an antibody heavy chain) encodes it operably (i.e., in a manner permitting its expression in a cell infected by a viral particle comprising the vector that contains the nucleic acid sequence). Additionally, the recombinant viral vectors of this invention are not limited to any particular configuration with respect to the exogenous protein-coding sequences. For example, in one embodiment of the present recombinant AAV vector, a “one vector” approach is used wherein a singular recombinant AAV vector includes nucleic acid sequences encoding both heavy and light antibody chains. In another embodiment, a “two vector” approach is used wherein one recombinant AAV vector includes a nucleic acid sequence encoding the heavy antibody chain, and a second recombinant AAV vector includes a nucleic acid sequence encoding the light antibody chain (See, e.g., S. P. Fuchs, et al.).
This invention provides a composition comprising (i) the present monoclonal antibody, and (ii) a pharmaceutically acceptable carrier.
This invention also provides a recombinant AAV vector comprising a nucleic acid sequence encoding the heavy chain and/or the light chain of the present monoclonal antibody.
In a preferred embodiment of the present recombinant AAV vector, the nucleic acid sequence encodes the heavy chain and the light chain of the present monoclonal antibody.
This invention further provides a recombinant AAV particle comprising the present recombinant AAV vector and an AAV capsid protein.
This invention still further provides a composition comprising (i) a plurality of the present AAV particles and (ii) a pharmaceutically acceptable carrier.
This invention provides a method for reducing the likelihood of a human subject's becoming infected with HIV-1 comprising administering to the subject a prophylactically effective amount of the present monoclonal antibody. Preferably, this method comprises administering the present monoclonal antibody according to a PrEP regimen. This invention also provides a method for reducing the likelihood of a human subject's becoming infected with HIV-1 comprising administering to the subject a prophylactically effective number of the present recombinant AAV particles. In the preferred embodiment of each of these prophylactic methods, the subject has been exposed to HIV-1.
This invention provides a method for treating a human subject who is infected with HIV-1 comprising administering to the subject a therapeutically effective amount of the present monoclonal antibody. This invention also provides a method for treating a human subject who is infected with HIV-1 comprising administering to the subject a therapeutically effective number of the present recombinant AAV particles. In the preferred embodiment of each of these therapeutic methods, the subject is symptomatic of an HIV-1 infection.
This invention provides a method for treating a human subject who is infected with SARS-CoV-2 comprising administering to the subject a therapeutically effective amount of the present monoclonal antibody. This invention also provides a method for treating a human subject who is infected with SARS-CoV-2 comprising administering to the subject a therapeutically effective number of the present recombinant AAV particles. In one embodiment of each of these therapeutic methods, the subject is symptomatic of a SARS-CoV-2 infection. In another embodiment, the subject is asymptomatic of a SARS-CoV-2 infection.
In addition to the prophylactic and therapeutic methods above, this invention provides prophylactic and therapeutic methods for addressing CCR5-mediated disorders generally. Specifically, this invention provides a method for reducing the likelihood of a human subject's becoming afflicted with a CCR5-mediated disorder comprising administering to the subject a prophylactically effective amount of the present monoclonal antibody. This invention also provides a method for treating a human subject who is afflicted with a CCR5-mediated disorder comprising administering to the subject a therapeutically effective amount of the present monoclonal antibody. This invention further provides a method for reducing the likelihood of a human subject's becoming afflicted with a CCR5-mediated disorder comprising administering to the subject a prophylactically effective number of the present recombinant AAV particles. This invention still further provides a method for treating a human subject who is afflicted with a CCR5-mediated disorder comprising administering to the subject a therapeutically effective number of the present recombinant AAV particles. In one embodiment of each of these prophylactic and therapeutic methods, the CCR5-mediated disorder is selected from the group consisting of hypercytokinemia, cytokine release syndrome, Alzheimer's disease, cancer, atherosclerosis, arthritis, inflammatory bowel disease, non-alcoholic steatohepatitis (NASH), graft-vs-host disease (GvHD), and multiple sclerosis. Preferably, the CCR5-mediated disorder is metastatic breast cancer.
The following are four exemplary embodiments of the present methods for addressing CCR5-mediated disorders. In a first embodiment, this invention provides a method for reducing the likelihood of a human subject's becoming afflicted with hypercytokinemia comprising administering to the subject a prophylactically effective amount of the present monoclonal antibody. Preferably, the subject is infected with a virus (e.g., SARS-CoV-2).
In a second embodiment, this invention provides a method for treating a human subject who is afflicted with hypercytokinemia comprising administering to the subject a therapeutically effective amount of the present monoclonal antibody. Preferably, the subject is infected with a virus (e.g., SARS-CoV-2).
In a third embodiment, this invention provides a method for reducing the likelihood of a human subject's becoming afflicted with hypercytokinemia comprising administering to the subject a prophylactically effective number of the present recombinant AAV particles. Preferably, the subject is infected with a virus (e.g., SARS-CoV-2).
In a fourth embodiment, this invention provides a method for treating a human subject who is afflicted with hypercytokinemia comprising administering to the subject a therapeutically effective number of the present recombinant AAV particles. Preferably, the subject is infected with a virus (e.g., SARS-CoV-2).
This invention provides methods for treating a human subject afflicted with a disorder (such as cancer) whereby the present monoclonal antibody or AAV particle is administered to the subject in conjunction with an agent for treating the disorder, wherein the agent is known to cause cytokine release syndrome. This either prevents the agent from causing cytokine release syndrome in the subject, or reduces the severity of any cytokine release syndrome that the agent causes.
Specifically, this invention provides a first method for treating a human subject afflicted with a disorder (such as cancer) comprising (i) administering to the subject a therapeutically effective amount of an agent for treating the disorder (e.g., a monoclonal antibody or an adoptive T-cell therapy) and (ii) administering to the subject a prophylactically effective amount of the present monoclonal antibody in conjunction with step (i), wherein the agent is known to cause cytokine release syndrome.
In a preferred embodiment of the first method, the disorder is selected from the group consisting of non-Hodgkin's lymphoma (NHL), chronic lymphocytic leukemia (CLL), and rheumatoid arthritis, and the agent is Rituxan® (rituximab). In another preferred embodiment, the disorder is selected from the group consisting of B-cell precursor acute lymphoblastic leukemia, diffuse large B-cell lymphoma (DLBCL) not otherwise specified, high grade B-cell lymphoma, and DLBCL arising from follicular lymphoma, and the agent is Kymriah® (tisagenlecleucel).
In the first method, administering the present monoclonal antibody to the subject in conjunction with the agent includes, for example, (i) administering the present monoclonal antibody to the subject before administering the agent, (ii) administering the present monoclonal antibody to the subject concurrently with the agent (e.g., as an admixture or via separate infusions), or (iii) administering the present monoclonal antibody to the subject immediately after administering the agent. In one embodiment, administering the present monoclonal antibody to the subject in conjunction with the agent includes (i) and (ii) above; (ii) and (iii) above; (i) and (iii) above; or (i), (ii), and (iii) above. Preferably, the first method prevents cytokine release syndrome in the subject, or at least reduces its severity.
This invention also provides a second method for treating a human subject afflicted with a disorder (such as cancer) comprising (i) administering to the subject a therapeutically effective amount of an agent for treating the disorder (e.g., a monoclonal antibody or an adoptive T-cell therapy) and (ii) administering to the subject a prophylactically effective number of the present AAV particles in conjunction with step (i), wherein the agent is known to cause cytokine release syndrome.
In a preferred embodiment of the second method, the disorder is selected from the group consisting of non-Hodgkin's lymphoma (NHL), chronic lymphocytic leukemia (CLL), and rheumatoid arthritis, and the agent is Rituxan® (rituximab). In another preferred embodiment, the disorder is selected from the group consisting of B-cell precursor acute lymphoblastic leukemia, diffuse large B-cell lymphoma (DLBCL) not otherwise specified, high grade B-cell lymphoma, and DLBCL arising from follicular lymphoma, and the agent is Kymriah® (tisagenlecleucel).
In the second method, administering the present AAV particles to the subject in conjunction with the agent includes, for example, administering the present AAV particles to the subject before administering the agent. Preferably, the second method prevents cytokine release syndrome in the subject, or at least reduces its severity.
Finally, this invention provides three kits. The first kit comprises, in separate compartments, (a) a diluent and (b) a suspension of the present monoclonal antibody. The second kit comprising, in separate compartments, (a) a diluent and (b) the present monoclonal antibody in lyophilized form. The third kit comprises, in separate compartments, (a) a diluent and (b) a suspension of a plurality of the present recombinant AAV particles. In one example, the third kit comprises (i) a single-dose vial containing a concentrated solution of the present particles (also measured as viral genomes) in a suitable solution (e.g., a solution of sterile water, sodium chloride, sodium phosphate, and Poloxamer 188) and (ii) one or more vials of suitable diluent (e.g., a solution of sterile water, sodium chloride, sodium phosphate, and Poloxamer 188).
The present vectors, particles, and methods are envisioned for suitable recombinant non-AVV viruses (e.g., lentivirus, adenovirus, alphavirus, herpesvirus, or vaccinia virus), mutatis mutandis, as they are for recombinant AAV viruses in this invention.
The present compositions, vectors, viral particles, methods, and kits are envisioned for the IgM and IgA antibody classes of the present monoclonal antibodies, mutatis mutandis, as they are for the IgG antibody class in this invention.
This invention will be better understood by reference to the examples which follow, but those skilled in the art will readily appreciate that the specific examples detailed are only illustrative of the invention as described more fully in the claims which follow thereafter.
In a preferred embodiment, the terminal half-life of the present monoclonal antibody can be determined using the homozygous human FcRn transgenic mouse referred to as “Tg32” and described in Avery, et al. Specifically, a single dose (e.g., 10 mg/kg) of the present monoclonal antibody is intravenously administered to the Tg32 mouse, and the antibody's terminal half-life in the mouse is determined. Preferably, a single dose (e.g., 10 mg/kg) of the present monoclonal antibody and a single dose of the same amount of PRO 140 are separately (e.g., concurrently) intravenously administered to Tg32 mice, so that the terminal half-life of each can be determined and compared. Methods for determining terminal half-life based on in vivo data are known and are described, for example, in D. Sheridan, et al. By way of example, plasma terminal half-life can be determined using Pharsight Phoenix WinNonlin software.
In a preferred embodiment of this invention, the present monoclonal antibodies are prophylactically administered according to a PrEP regimen in order to more effectively lower the likelihood of a subject's becoming infected with HIV during a high-risk event such as sex or intravenous drug use.
Subjects
In one embodiment, a subject who would benefit from a PrEP regimen using the present monoclonal antibodies includes a sexually active individual who has engaged in anal or vaginal sex within six months of initiating a PrEP regimen, and (i) has an HIV-positive sexual partner, and/or (ii) has a history inconsistent condom use, or no condom use, with sexual partners.
Dosages
A suitable amount of the present monoclonal antibody is administered as a single 3 ml subcutaneous injection. In one embodiment, the initial dose is followed by a second dose after two weeks, and by further doses every two weeks thereafter, with follow-up visits as appropriate. In another embodiment, the initial dose is followed by a second dose after one month, and by further doses every two months thereafter, with follow-up visits as appropriate. In another embodiment, the initial dose is followed by a second dose after two months, and by further doses every three months thereafter, with follow-up visits as appropriate. In a further embodiment, the initial dose is followed by a second dose after three months, and by further doses every three months thereafter, with follow-up visits as appropriate. In yet a further embodiment, the initial dose is followed by a second dose after six months, and by further doses every six months thereafter, with follow-up visits as appropriate.
The suitable amount of the present monoclonal antibody includes, without limitation, any of the following: (i) 0.1 mg, 0.5 mg, 1 mg, 5 mg, 10 mg, 15 mg, 20 mg, 25 mg, 30 mg, 35 mg, 40 mg, 45 mg, 50 mg, 60 mg, 70 mg, 80 mg, 90 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, 225 mg, 250 mg, 275 mg, 300 mg, 325 mg, 350 mg, 375 mg, 400 mg, 425 mg, 450 mg, 475 mg, 500 mg, 525 mg, 550 mg, 575 mg, 600 mg, 625 mg, 650 mg, 675 mg, 700 mg, 725 mg, 750 mg, 775 mg, 800 mg, 825 mg, 850 mg, 875 mg, 900 mg, 925 mg, 950 mg, 975 mg, 1,000 mg, 1,250 mg, 1,500 mg, 1,750 mg, 2,000 mg, 2,250 mg, 2,500 mg, 2,750 mg, 3,000 mg, 3,250 mg, 3,500 mg, 3,750 mg, 4,000 mg, 4,250 mg, 4,500 mg, 4,750 mg, 5,000 mg, 5,250 mg, 5,500 mg, 5,750 mg, 6,000 mg, 6,250 mg, 6,500 mg, 6,750 mg, 7,000 mg, 7,250 mg, 7,500 mg, 7,750 mg, 8,000 mg, 8,250 mg, 8,500 mg, 8,750 mg, 9,000 mg, 9,250 mg, 9,500 mg, 9,750 mg, or 10,000 mg; (ii) 0.1 mg to 1 mg, 1 mg to 5 mg, 5 mg to 20 mg, 20 mg to 50 mg, 50 mg to 100 mg, 100 mg to 150 mg, 150 mg to 200 mg, 200 mg to 250 mg, 250 mg to 300 mg, 300 mg to 350 mg, 350 mg to 400 mg, 400 mg to 450 mg, 450 mg to 500 mg, 500 mg to 550 mg, 550 mg to 600 mg, 600 mg to 650 mg, 650 mg to 700 mg, 700 mg to 750 mg, 750 mg to 800 mg, 800 mg to 850 mg, 850 mg to 900 mg, 900 mg to 950 mg, 950 mg to 1,000 mg, 1,000 mg to 1,250 mg, 1,250 mg to 1,500 mg, 1,500 mg to 1,750 mg, 1,750 mg to 2,000 mg, 2,000 mg to 2,250 mg, 2,250 mg to 2,500 mg, 2,500 mg to 2,750 mg, 2,750 mg to 3,000 mg, 3,000 mg to 3,250 mg, 3,250 mg to 3,500 mg, 3,500 mg to 3,750 mg, 3,750 mg to 4,000 mg, 4,000 mg to 4,250 mg, 4,250 mg to 4,500 mg, 4,500 mg to 4,750 mg, 4,750 mg to 5,000 mg, 5,000 mg to 5,250 mg, 5,250 mg to 5,500 mg, 5,500 mg to 5,750 mg, 5,750 mg to 6,000 mg, 6,000 mg to 6,250 mg, 6,250 mg to 6,500 mg, 6,500 mg to 6,750 mg, 6,750 mg to 7,000 mg, 7,000 mg to 7,250 mg, 7,250 mg to 7,500 mg, 7,500 mg to 7,750 mg, 7,750 mg to 8,000 mg, 8,000 mg to 8,250 mg, 8,250 mg to 8,500 mg, 8,500 mg to 8,750 mg, 8,750 mg to 9,000 mg, 9,000 mg to 9,250 mg, 9,250 mg to 9,500 mg, 9,500 mg to 9,750 mg, or 9,750 mg to 10,000 mg; (iii) 0.1 mg/kg, 0.2 mg/kg, 0.3 mg/kg, 0.4 mg/kg, 0.5 mg/kg, 0.6 mg/kg, 0.7 mg/kg, 0.8 mg/kg, 0.9 mg/kg, 1 mg/kg, 2 mg/kg, 3 mg/kg, 4 mg/kg, 5 mg/kg, 6 mg/kg, 7 mg/kg, 8 mg/kg, 9 mg/kg, 10 mg/kg, 15 mg/kg, 20 mg/kg, 25 mg/kg, 30 mg/kg, 35 mg/kg, 40 mg/kg, 45 mg/kg, 50 mg/kg, 60 mg/kg, 70 mg/kg, 80 mg/kg, 90 mg/kg, 100 mg/kg, 200 mg/kg, 300 mg/kg, 400 mg/kg, or 500 mg/kg; or (iv) 0.1 mg/kg to 1 mg/kg, 1 mg/kg to 10 mg/kg, 10 mg/kg to 20 mg/kg, 20 mg/kg to 30 mg/kg, 30 mg/kg to 40 mg/kg, 40 mg/kg to 50 mg/kg, 50 mg/kg to 60 mg/kg, 60 mg/kg to 70 mg/kg, 70 mg/kg to 80 mg/kg, 80 mg/kg to 90 mg/kg, 90 mg/kg to 100 mg/kg, 100 mg/kg to 200 mg/kg, 200 mg/kg to 300 mg/kg, 300 mg/kg to 400 mg/kg, or 400 mg/kg to 500 mg/kg.
In a preferred embodiment of this invention, the prophylactic efficacy of the present monoclonal antibodies is tested in macaques using the following method taken from Chang, et al.
HIV and SHIV Stocks
HIV-1 isolates are obtained from the NIH AIDS Reagent Program, with the majority from the HIV-1 60 International Isolate Panel (Cat #11412). The SHIVSF162P3 stock (173.3 ng/mL p27, 1×109 vRNA copies/mL, 2.67×105 TCID50/mL in TZM-bl cells, 1.28×103 TCID50/mL in rhesus PBMC (peripheral blood mononuclear cells)) is used in the PrEP animal study.
HIV and SI-11V In Vitro Infection Assays
Human PBMCs are first depleted of CD8+ T cells with Human CD8 Microbeads (Miltenyi) and then sequentially enriched for CD4+ T cells with Human CD4 Microbeads (Miltenyi) following the manufacturer's instructions. The resulting CD4+CD8− T cells are incubated at 2×106 cells/mL in R15-100 media (RPMI 1640 with antibiotic/mycotic, 15% fetal bovine serum (FBS), and 100 U/mL IL-2) and activated for 24 hours with a stimulating cocktail containing CD3, CD49d, CD28 antibodies (BD Biosciences), and Staphylococcal enterotoxin B (Toxin Technologies, Inc.). After 24 hours, cells are washed two times and incubated for 2-3 additional days in R15-100 before viral infection. At day 0 of infection, 5×105 cells are incubated with or without the desired concentration of mAb (i.e., the present monoclonal antibody) for 1 hour at 37° C. Next, cells are infected with the desired HIV isolate by spinoculation for 2 hours at 1200×g at room temperature (RT). Cells are washed four times with R15-100 to remove free viruses and cultured with R15-100 media plus the same concentration of mAb used during the pre-treatment step. An additional 5×105 cells are left uninfected and kept in culture as the uninfected control. Cultures are maintained by replacing 50% of the culture with new media containing the desired concentration of mAb every other day for 5 days, when cells are harvested for intracellular p24 staining by flow cytometry.
Rhesus macaque (RM) PBMCs are depleted of CD8+ T cells by staining with NHP CD8-PE (Miltenyi) followed by anti-PE microbeads (Miltenyi) then subsequently enriched for CD4+ T cells with NHP CD4 microbeads (Miltenyi), following the manufacturer's instructions. Purified RM CD4+ cells are activated and maintained in culture similarly to human CD4+ T cells, as described above. For the spreading assay with HIV-1 Ba-L, HIV-1 LAI, and SHIVSF162P3, CD4+ T cells are isolated, activated, infected, and cultured as described above but with the following changes. HIV-1 Ba-L, HIV-1 LAI, and SHIVsn62p3 are used to infect at a multiplicity of infection (MOI) of 1×10−5 (FFU/cell) and kept in culture for 6 days before p24 or p27 intracellular staining. For detection of intracellular HIV p24 or SHIV p27 by flow cytometry, cells are stained for CD3, CD4, CD8, and amine-reactive dye for viability for 30 minutes at RT in the dark. Cells are washed once with phosphate-buffered saline (PBS), spun down at 830×g for 4 minutes, and fixed with 2% paraformaldehyde (PFA) for 30 minutes in 4° C. Afterwards, cells are washed once with FACS buffer (10% bovine growth serum in PBS) and stained with p24 or p27 antibodies in 100 μL of Permeabilization Medium B (Thermo Fisher) for 1 hour at RT in the dark. Cells are washed once with FACS buffer and fixed again with 2% PFA for more than 30 minutes before collecting on LSR-II instrument and FACsDIVA version 6.1 (BD Biosciences, Franklin Lakes, N.J.). Data are analyzed using FlowJo v10 (Tree Star) by gating on singlet, live, CD3+, CD8−, and p24+ or p27+.
Human Blood Donors
Healthy human donor whole blood is purchased from Innovative Research and further processed to PBMC by density gradient centrifugation using Ficoll-Hypague. Blood is collected with K2 EDTA anticoagulant and tested negative for the following viral markers: HIV-1 RNA, antibodies to HIV, antibodies to hepatitis C virus (HCV), HCV RNA, hepatitis B virus (HBV) DNA, hepatitis B surface antigen (HbsAg), and syphilis. CCR5 expression is confirmed via flow cytometry. Leukapheresis samples are collected from TRB following the relevant informed consent procedures.
Quantitation of CCR5 Expression Levels
To measure the frequency of CCR5-expressing cells, PBMCs are incubated with 5 μg/mL of unlabeled mAb for 30 minutes at RT in the dark, and then washed once with PBS. Anti-human IgG4 is used as a secondary antibody to detect surface-bound mAb for 30 minutes at RT in the dark. Cells are washed once with FACS buffer and once with PBS, and then stained for CD3, CD4, CD8, CCR5 (via antibody clone 3A9, which is specific to a distinct, non-competitive CCR5 epitope than mAb), and amine-reactive dye for 30 minutes at RT in the dark. Cells are washed twice with PBS and fixed with 2% PFA before collecting through the LSR-II instrument and FACsDIVA version 6.1. Samples are analyzed by gating on singlet, live, CD3+, CD4+/CD8−, and CCR5+(via clone 3A9) and/or human IgG4+ events. The number of CCR5 molecules on the cell surface is measured with quantitative cytometry using the Quantum Molecules of Equivalent Soluble Fluorochrome (MESF) kit (Bangs Laboratories, Inc). PE-conjugated mAb used to quantify surface CCR5 expression and PE-labeled microspheres for standard curve generation are provided by IncelIDX. Phenotypic staining is done using CD3−, CD4−, CD8−, CD14−, CD16− specific antibodies used as described above. T cell memory subset determination is defined as central memory (human: CCR7+CD45RA−, RM: CD28+CD95+), effector memory (human: CCR7−CD45RA−, RM: CD28−CD95+), and naive (human: CCR7+CD45RA+, RM: CD28+CD95−). Gating is done using FlowJo v10. MFI is used to attain MESF according to the manufacturer's protocol.
mAb CCR5 RO
To measure the percentage of CCR5 RO on the surface of CD4+ T cells, the following RO equation can be employed:
% RO=[% IgG4/(% IgG4+% mAb-PB)]×100%.
The equation measures unoccupied CCR5 receptors by using Pacific Blue-conjugated mAb (termed mAb-PB). CCR5 RO is defined as the percentage of cells CCR5+(measured by clone 3A9) and mAb+(measured by anti-human IgG4) divided by the percentage of cells CCR5+ and mAb+(measured by the sum of anti-human IgG4 and mAb-PB) cells following incubation with a saturating concentration of mAb-PB. This method is based on RO assays for anti-PD-1 antibodies in clinical trials.
PBMC or single cells from tissue homogenates (0.3-1×106) are stained with anti-human IgG4 for 30 minutes at RT in the dark. Next, cells are washed once with FACS buffer and three times with PBS and then stained with CD45, CD3, CD4, CD8, CD16, CD14, amine-reactive dye, CCR5 (via antibody clone 3A9), and mAb-PB for 30 minutes at RT in the dark. Finally, cells are washed twice with PBS and fixed with 2% PFA for more than 30 minutes before collecting on LSR-II instrument and FACsDIVA version 6.1. Using FlowJo v10, cells are gated on CD45+, singlet, live, CD3+, CD4+, and CCR5+(via 3A9 staining) events. The CD4+ CCR5+ population is further gated on human IgG4+ or mAb-PB+ events.
Rhesus Macaques
All study RMs are housed in ABSL-2+ rooms with autonomously controlled temperature, humidity, and lighting. At assignment, all study RM are free of cercopithicine herpesvirus 1, D-type simian retrovirus, simian T-lymphotrophic virus type 1, and Mycobacterium tuberculosis. RMs are typed for the MHC alleles Mamu-Aantibody01, Mamu-Aantibody02, Mamu-Bantibodyl7, and Mamu-Bantibody08, with Mamu-Bantibodyl7 and/or -Bantibody08 positive animals excluded when possible or placed into control groups when not possible to exclude biasing results. All attempts are made to pair housed RM during the study period. When RMs are single cage-housed due to infection status, they had visual, auditory, and olfactory contact with other animals, and an enhanced enrichment plan is designed and overseen by RM behavior specialists. RMs are fed commercially prepared primate chow twice daily and receive supplemental fresh fruit or vegetables daily. Fresh, potable water is provided via automatic water systems. RMs are sedated with ketamine HCl or dexmedetomidine for procedures, including subcutaneous mAb administration, venipuncture, BAL, tissue biopsy, and SHIV challenge. For spleen and mesenteric lymph node biopsies, animals are sedated with isoflurane. At scheduled endpoints, RMs are euthanized with sodium pentobarbital overdose (>50 mg/kg) and exsanguinated via the distal aorta. A certified veterinary pathologist performs tissue collection at necropsy. The relevant authorities should approve RM care and all experimental protocols and procedures.
For the mAb PrEP study, a total of 18 adult RMs are used and divided between three experimental groups: (1) six RMs in the control group, (2) six RMs in the 10 mg/kg weekly mAb-treated group, and (3) six RMs in the 50 mg/kg every 2 weeks mAb-treated group. The three groups are gender matched, with two females and four male RMs in each group. Animals receive their first injection of mAb 1 week before the initiation of viral challenges, and continue to receive mAb until study week 7, for a total of nine mAb injections for group 1 (10 mg/kg treated group) and a total of five mAb injections for group 2 (50 mg/kg treated group). At study week 0, all three groups receive their first IR SHIVSF162P3 challenge with a 1:400 dilution delivered via atraumatic installation into the rectum with a needleless syringe. Viral challenge then continues every week until confirmed infection or until study week seven for a total of eight consecutive weekly IR challenges. Animals are euthanized as described above, with the timing dictated by the following guidelines: (1) 10 weeks after acquisition of SHIVSF162P3 infection, which is confirmed by plasma viremia, or (2) 8 weeks after complete washout of plasma mAb and loss of mAb CCR5 RO on CD4+ T cells in blood. Three SHIV-naïve RMs serve as adoptive transfer recipients for tissue homogenates from PrEP RM as described. All mAb used in these studies is clinical-grade material provided at a concentration of 175 mg/mL.
Processing of Blood and Tissue
Whole blood is collected into EDTA-treated or non-anticoagulant tubes (BD Biosciences). Blood in EDTA-tubes is assessed for complete blood counts using an ABX Pentra 60C+ Hematology Analyzer. Blood in non-anticoagulant tubes is spun down at 1860×g for 10 minutes to separate serum from clotted blood, and then assessed for serum chemistry values using an ABX Pentra 400 Chemistry Analyzer. PBMCs and plasma are isolated from whole blood in EDTA-treated tubes by density gradient centrifugation using Ficoll-Hypaque. Bronchoalveolar lavage fluid is collected in PBS and filtered through 70-μm cell strainers. Lymph node and spleen are collected in RPMI 1640 containing 10% FBS (R10), diced into tiny pieces with a scalpel, and forced through 70-μm cell strainers to collect single-cell suspensions. Bone marrow is collected in R10, pelleted by spinning at 830×g for 4 minutes. Cell pellet is resuspended by vigorously shaking in PBS containing 2 nM EDTA. Cell suspension is spun down again at 830×g for 4 minutes and resuspended in 70% isotonic Percoll (GE Healthcare, Buckinghamshire, UK) before underlaying in 37% isotonic Percoll. Cells are spun at 500×g for 20 minutes with brake. Mononuclear cells in the center interface are collected and washed in R10. Duodenum and colon are collected in R10 and diced into tiny pieces with a scalpel. Tissues are placed into a 50 mL conical tube containing R3 (RPMI 1640 and 3% FBS) with 0.5 M EDTA, and then incubated in 37° C. with 225 r.p.m. shaking for 30 minutes to remove mucus coating. After 30 minutes, cells are poured over a tea strainer and sequentially washed three times with Hank's Buffered Salt Solution (HBSS) to remove EDTA and mucus coating. Tissues are returned into a 50 mL conical tube containing R3, 0.2 mg/mL collagenase (Sigma-Aldrich, St. Louis, Mo.), and 0.2 mg/mL DNase I (Roche, Indianapolis, Ind.), and then incubated in 37° C. with 225 r.p.m. shaking for 1 h. Digested tissues are filtered through a 70-μm cell strainers. Cell flow-throughs are spun at 840×g for 4 minutes. Pellet is resuspended in 70% isotonic Percoll and underlaid in 37% isotonic Percoll, before spinning at 500×g for 20 minutes with brake. Mononuclear cells in the center interface are collected and washed in R10.
To quantify mAb concentration and ADA in the blood, plasma is heat-inactivated by incubation at 56° C. for 30 minutes, and then spun for 20 minutes at 12,000×g to pellet residual debris. Resulting supernatant is transferred to a new tube and stored at −80° C. until assayed. To quantify mAb concentration in tissues, tissues are finely diced and 10-1,000 mg of tissue of interest are placed into Lysing Matrix tubes (MP Biomedicals) and 300-500 μL of complete EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich) in PBS is added. Tissue disruption is achieved by beating in a Beadbeater (Biospec) device for three cycles of 1 minute beating and 1 minute on ice. Supernatant from the tissue homogenate is transferred to a new tube and spun for 20 minutes at 12,000×g to pellet residual debris. Resulting supernatant is transferred to a new tube and stored at −80° C. until assayed.
Viral Nucleic Acid Detection
Nucleic acids from plasma and PBMC cell pellets are extracted using the Maxwell 16 instrument (Promega, Madison, Wis.) following the manufacturer's protocol, which uses the LEV Viral Nucleic Acid Kit for plasma and the LEV Whole Blood Nucleic Acid kit for cell pellets.
Nucleic acid from tissues is extracted by first placing tissues in Lysing Matrix tubes (MP Biomedicals) with 1 mL Tri-reagent (Molecular Research Center, TR-118). Then, it is vortexed to soaked tissues in Tri-reagent and placed on wet ice. Tissues are grinded using the MagNA Lyser rotor (Roche Life Science) for 1-2 cycles depending on the size of the tissue, alternating between the MagNA Lyser rotor and wet ice. Tissue homogenates are briefly spun down to pellet the beads and supernatant is pipetted out for nucleic acid extraction. Supernatant is mixed with 1/10 bromochloropropane (BCP) to Tri-reagent volume. Mixture is vortexed and incubated for 5 minutes in room temperature, before spinning at 12,000×g for 15 minutes at 4° C. to achieve phase separation. For RNA extraction, the upper aqueous layer is pipetted into a new tube containing 12 μL of glycogen (Thermo Scientific) and 0.5 mL isopropanol. The sample is inverted to mix and spun at 15,000×g for 10 minutes in room temperature. Isopropanol is carefully discarded without losing pellet, and then it is washed with 0.7 mL 75% ethanol twice by spinning at 15,000×g for 10 minutes at room temperature. After discarding the ethanol, the RNA pellet is dried at room temperature for 10-15 minutes. Finally, RNA resuspension buffer (10 mM Tris, pH 8) is added to the RNA-containing tube and incubated in a 37° C. heat block for 15 minutes to elute. Resuspended RNA is stored at −80° C. until assayed. For DNA extraction, the interphase and organic layers, not used for RNA extraction, are mixed with 0.5 mL of DNA extraction buffer, containing 4 M guanidine thiocyanate (Sigma), 50 mM sodium citrate (Sigma), and 1 M Tris base. The reaction is vortexed and incubated for 5 minutes in room temperature, before spinning at 12,000×g for 15 minutes at 4° C. to achieve phase separation. DNA-containing upper aqueous layer is pipetted into a new tube containing 12 μL of glycogen (Thermo Scientific) and 0.4 mL isopropanol. The remaining steps follow RNA extraction protocol described above, with the exception of the DNA resuspension buffer (10 mM Tris, pH 9) used to elute the DNA pellet.
Viral copies are measured by quantitative reverse transcription PCR (RT-qPCR) or qPCR that targets a highly conserved sequence of Gag. The assays use the SGAG21 forward primer (GTCTGCGTCATPTGGTGCATTC), SGAG22 reverse primer (CACTAGKTGTCTCTGCACTATPTGTTTTG), and pSGAG23 probe (5′-6-carboxyfluorescein [FAM]-CTTCPTCAGTKTGTTTCACTTTCTCTTCTGCG-black hole quencher [BHQ1]-3′). All viral detection assays are performed by those who are blinded regarding the treatment conditions of each animal.
To quantitate SHIV viral RNA in plasma, RT-qPCR reactions are performed using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). The reactions use the total RNA extracted from 300 μL of plasma, with 900 nM SGAG21, 900 nM of SGAG22, and 250 nM pSGAG23 in a final volume of 30 μL. Viral RNA copies per reaction are calculated with a standard curve created by using in vitro transcribed SIVgag RNA that is serially diluted in 5 ng/μL yeast tRNA (Sigma R5636). SIV-positive plasma RNA is used as a positive control and nuclease-free water is used as a negative control. Reactions are run with the Applied Biosystems QuantStudio 6 Flex instrument (Life Technologies) using the following thermal conditions: 50° C. for 5 minutes; 95° C. for 20 seconds; [95° C. for 3 seconds, 60° C. for 30 seconds]×45 cycles. The limit of quantification for this assay is 50 copies/mL.
To detect SHIV viral RNA copies in cell pellets and tissues, a two-step RT-qPCR reaction is performed, where 2.5 μg RNA is synthesized to complementary DNA (cDNA) using 20 U Superscript II RT (Thermo Fisher/Invitrogen), 5 mM MgCl2, 0.5 mM dNTPs, 1 mM dithithreitol (DTT), 150 ng random hexamers, 1×TaqMan PCR buffer (with 0.05% gelatin and 0.02% Tween-20), and 20 U RNAaseOut in a final volume of 30 μL. The reaction is performed with an Applied Biosystems ABI 9700 instrument using the following thermal conditions: 25° C. for 15 minutes, 42° C. for 40 minutes, 90° C. for 15 minutes, 25° C. for 30 minutes, and 5° C. hold. Following reverse transcription, qPCR is performed by adjusting the reaction to contain 1.25 U Platinum Taq (Applied Biosystems), 600 nM of SGAG21, 600 nM of SGAG22, 100 nM pSGAG23, 1× TaqMan PCR II buffer, 4.5 mM MgCl2, and 50 nM ROX passive reference dye, in a final volume of 50 μL. Viral RNA copies per reaction are calculated with a standard curve created by using in vitro transcribed SIVgag RNA that is serially diluted in 100 ng/μL yeast tRNA (Sigma R5636). Reactions are performed in an Applied Biosystems ABI 7500 instrument using the following thermal condition: 95° C. for 2 minutes; [95° C. for 15 seconds, 60° C. for 1 minute]×45 cycles. To detect SHIV viral DNA copies in cell pellets and tissues, qPCR reactions are done using Taqman Fast Advanced Master Mix (Life Technologies). Extracted DNA is first heated at 95° C. for 5 minutes and then placed on ice. The reactions used 2.5 μg of DNA, with 600 nM of SGAG21, 600 nM of SGAG22, and 100 nM pSGAG23. Viral DNA copies per reaction are calculated with a standard curve created with linearized plasmid DNA containing the SIVgag sequence that is serially diluted in TE buffer containing 2.5 ng/mL SIV-negative rhesus genomic DNA as carrier. Reactions are run with the Applied Biosystems QuantStudio 6 Flex instrument (Life Technologies) using the following thermal conditions: 50° C. for 2 minutes; 95° C. for 20 seconds; [95° C. for 1 second, 60° C. for 20 seconds]×45 cycles. The limit of quantification for this assay is 10 copies/million cells for cell pellets and 7 copies/million cells for whole tissue biopsies.
mAb Measurement in Plasma and Tissues
Enzyme-linked immunosorbent assay (ELISA) is used to detect free mAb in plasma. Half-area 96-well Costar Assay Plates (Corning) are coated with anti-idiotype antibody at 1.5 μg/mL in carbonate-bicarbonate buffer (Thermo Fisher) and incubated overnight. Plates are washed three times with PBS-T (PBS+0.1% Tween-20) and blocked with blocking buffer (PBS+0.4% Tween-20+10% BSA) for at least 2 hours at RT. mAb concentration is calculated with a standard curve created with a serial titration of mAb diluted in blocking buffer with a range of 4.7-300 ng/m L. Heat-inactivated plasma samples are also diluted with blocking buffer. After incubating for 30 minutes at RT, plates are washed three times with 0.5 M NaCl in PBS and incubated with 1:20,000 dilution of mouse anti-human IgG4pFc′-horseradish peroxidase (HRP) (Southern Biotech) in blocking buffer for 30 minutes at RT. Plate is washed three times with PBS-T and developed for 2 minutes using 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Southern Biotech). Reaction is stopped with 1 N H2SO4. Plates are read on the Synergy HTX Multi-Mode Microplate Reader (BioTek) and data are collected using software Gen5 v3.09 at two absorbance wavelengths: 650 nm for the developing reaction and 450 nm for the developed reaction after the reaction is stopped with 1 N H2SO4. Final OD is determined by OD450 nm minus OD650 nm. The limit of detection for the assay is 22.5 ng/m L. mAb in tissue is quantified in supernatants prepared from tissue homogenates by ELISA as described above. Further, the mass of the total protein in the collected tissues is determined by the Pierce Coomassie Plus Broadford Assay (Thermo Fisher) following the manufacturer's instructions. Tissue concentration of mAb is reported as the ng of mAb per mg of total protein.
Measurement of Antibody Antidrug Antibodies (ADA)
Half-area 96-well Costar Assay Plates (Corning) are coated with 2 μg/mL mAb. Plates are washed with PBS-T three times and blocked with blocking buffer for 2 hours at RT. Plates are then washed three times with PBS-T. Heat-inactivated plasma samples are serially diluted in blocking buffer, added to the plates in duplicate, and incubated at RT for 30 minutes. Plates are then washed three times with 0.5 M NaCl in PBS. To determine ADAs from RM, a secondary antibody recognizing rhesus IgG (anti-rhesus IgG1/3[1 B3]-HRP, NHP Reagent Resource) and conjugated to HRP is added. Plates are incubated at RT for 30 minutes, and then washed three times with PBS-T. TMB solution (Southern Biotech) is added at RT for 2 minutes and the reaction is stopped with 1 N H2SO4. Absorbance is read at 450 nm on a Synergy plate reader (BioTek). ADA titers are defined as the reciprocal of the highest dilution of the sample that yields a positive result (e.g. dilution of 1/2460=titer of 2460). A positive result is defined as twice that of background values.
Env Sequencing
Viral sequencing and analysis are adapted from previously published genome-wide SIVmac239 sequencing protocols. Viral RNA is isolated from virus stocks and plasma samples using QIAamp MinElute Virus Spin Kit following the manufacturer's instructions. Complementary DNA is generated with the SuperScript III One-Step RT-PCR with Platinum Taq (Thermo Fisher). SHIV envforward primer (GGCATAGCCTCATAAAATATCTG) and the SHIV env reverse primer (ACAGAGCGAAATGCAGTGATATT) are used to amplify a ˜4.5 kb amplicon spanning the env gene. RT-PCR reactions are performed on Eppendorf Mastercycler Pro S Thermal Cyclers using the following thermal conditions: 50° C. for 30 minutes; 94° C. for 2 minutes; [94° C. for 15 seconds, 60° C. for 1 minute, 68° C. for 4 minutes]×2 cycles; [94° C. for 15 seconds, 58° C. for 1 minute, 68° C. for 4 minutes]×2 cycles; [94° C. for 15 seconds, 60° C. for 1 minute, 68° C. for 4 minutes]×45 cycles; 68° C. for 10 minutes; and hold at 4° C. The resulting 4.9 kb fragments are purified on a 1% agarose gel and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Dual-indexed Illumina MiSeq-compatible libraries are then prepared using the Nextera XT DNA Sample Prep Kit, and purified with AMPure XP magnetic beads (Beckman Coulter). Libraries are analyzed on an Agilent 2100 Bioanalyzer using the HS DNA kit (Agilent), normalized to 2 nM, pooled at an equimolar ratio, and sequenced in parallel on the Illumina MiSeq. Sequence reads are processed, where raw data are trimmed using Trimmomatic version 0.39 and aligned to the SHIVSF162 reference sequence (GenBank Accession No. KF042063.1) using BWA-MEM version 0.7.17-r1188. All bases of the alignment are evaluated and single-nucleotide polymorphisms (SNPs) and deletion/insertion polymorphisms are called for bases with a quality score above 17. Importantly, the identity of the associated read is retained for each SNP, which allowed the phase of SNPs to be considered. This information allows amino acid translations to be calculated based on the sequence of each individual read, as opposed to the consensus sequence. SNP analysis and visualization of mutations is performed using the SequenceAnalysis module, written for LabKey Server 21.3.
T Cell Assays
SHIV-specific CD8+ T cell responses in PBMC are measured by flow cytometric intracellular cytokine staining (ICS). 1×106 PBMCs are incubated with overlapping 15-mer peptide pools spanning SIVmac239 Gag or Vif open reading frame and co-stimulated with CD28 and CD49d antibodies (eBiosciences) for 1 hour, followed by the incubation with Brefeldin A (Sigma-Aldrich) for an additional 8 hours. Stimulation with rhesus cytomegalovirus (RhCMV) lysate serves as a positive control while incubation without antigen serves as a background control. Cells are surface stained with antibodies for CD3, CD4, CD8, and amine-reactive dye, fixed with 2% PFA, permeabilized with BD FACS Lysing Solution (BD Biosciences), and stained intracellularly for IFN-γ, TNF-α, and CD69. Samples are collected on LSR-II instrument and FACsDIVA version 6.1 and analyzed with FlowJo v10 (Tree Star) by gating on singlet, live, CD3+, CD4−, and CD8+ cells. Responding CD8+ T cells are measured by Boolean gating on cells that are CD69+/TFN-α+ and/or CD69+/IFN-γ+.
Adoptive Transfer
To confirm sterilizing protection, cells from the infected control animals (all six animals) or uninfected mAb-treated animals (four animals in the 10 mg/kg group and all six animals in the 50 mg/kg group) are adoptively transferred into one SHIV-naïve RM per animal group. Cells are prepared an hour before infusion by resuspending in 1 mL of Hank's Buffered Salt Solution (HBSS) with 15 U/mL heparin. Recipient RM are sedated with ketamine HCl (8-20 mg/kg) or Telazol (2-5 mg/kg) and prophylactically treated with Benadryl (5 mg/kg) prior to infusion of donor cells. Donor cells are slowly infused intravenously with an infusion pump at a maximum rate of 22 mL/kg/h. Animals are monitored for at least 2 hours for post-procedural complications.
Antibodies
The following conjugated antibodies can be used in these studies: (a) from BD Biosciences, D058-1283 (CD45; PE Cy7; 1:100; cat #561294), SP34-2 (CD3; Alexa 700; 1:100; cat #557917), SP34-2 (CD3; PE; 1:20; cat #552127), LP200 (CD4; PerCP-Cy5.5; 1:40; cat #552838), RPA-T8 (CD8; PacBlu; 1:40; cat #558207), SK1 (CD8; TruRed; 1:100; cat #341051), 3 GB (CD16; Alexa 700; 1:100; cat #560713), 25723.11 (IFN-γ; APC; 1:100; cat #502512), 6.7 (TNF-α; PE; 1:100; cat #554513), 3A9 (CCR5; APC; 1:100; cat #560748), SK1 (CD8; BUV737; 1:50; cat #612754), L200 (CD4, BUV395; 1:200; cat #564107), FN50 (CD69; PE-Texas Red; 1:100; cat #562617), SP34-2 (CD3; Pacific Blue; 1:100; cat #558124); (b) from BioLegend, OKT4 (CD4; APC-Cy7; 1:100; cat #305612), RPA-T4 (CD4; APC; 1:100; cat #300537); (c) from Beckman Coulter, RM052 (CD14; PE-Texas Red; 1:40; cat #IM2707U), KC57 (HIV Gag p24; FITC; 1:100; cat #6604665); (d) from Sigma, HP-6025 (IgG4; FITC; 3:100; cat #F9890); and (e) from SouthernBiotech, HP6023 (mouse anti-human IgG4 pFc′; HRP; 1:20,000; cat #9190-05); and (f) from NHP Reagent Resource, 1 B3 (anti-rhesus IgG1/3; HRP; 1:5000). The following unconjugated antibodies can be used: 55-2F12 (SIV Gag p27; NIH AIDS Research and Reference Reagent Program), conjugated in-house to FITC using Pierce™ FITC Antibody Labeling Kit (Thermo Fisher) and used at approximately 1:100 depending on the efficacy of conjugation. Live/dead Fixable Yellow Dead Cell Stain Kit and Near-IR Dead Cell Stain Kit (Thermo Fisher) are amine-reactive dyes that can be used at 1:1000 dilution to assess cell viability.
Statistical Analyses
Time to infection is assessed by log-rank test. Differences in CCR5 expression percentages are measured by the nonparametric Kruskal—Wallis test, and differences in the number of CCR5 surface molecules are assessed by nonparametric Mann—Whitney test. Statistical significance is determined at the significant alpha level of 0.05. Statistical analyses are conducted using GraphPad Prism software version 6.0 (GraphPad Software, La Jolla, Calif.).
These cassette components include a CMV enhancer/chicken beta-actin promoter and intron (or CAG); an SV40 polyadenylation signal (or SV40 polyA); antibody heavy and light chains; and a Furin F2A self-processing peptide cleavage site. The expression cassette is flanked by AAV serotype 2 inverted terminal repeats (ITR). In the cassette-containing bicistronic single-stranded AAV (ssAAV) vector, both the heavy and light chains are expressed from one open reading frame using a F2A self-processing peptide from FMD. The furin cleavage sequence “RKRR” for the cellular protease furin is added for removal of amino acids left on the heavy chain C-terminus following F2A self-processing. In one embodiment of this invention, the present rAAV vectors possess introns, and in another embodiment, they do not. Abbreviations: CMV, cytomegalovirus; SV40, simian virus 40; and FMD, foot-in-mouth disease virus.
The present rAAVs can be produced according to known methods. For instance, in one such method, HEK-293 cells are transfected with a select rAAV vector plasmid and two helper plasm ids to allow generation of infectious AAV particles. After harvesting transfected cells and cell culture supernatant, rAAV is purified by three sequential CsCl centrifugation steps. Vector genome number is assessed by Real-Time PCR, and the purity of the preparation is verified by electron microscopy and silver-stained SDS-PAGE. (Mueller, et al.)
This application claims the benefit of U.S. Provisional Application No. 63/042,661, filed Jun. 23, 2020, and U.S. Provisional Application No. 63/062,506, filed Aug. 7, 2020, the contents of both of which are incorporated herein by reference. Throughout this application, various publications are cited. The disclosure of these publications is hereby incorporated by reference into this application to describe more fully the state of the art to which this invention pertains.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2021/038349 | 6/22/2021 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 63042661 | Jun 2020 | US | |
| 63062506 | Aug 2020 | US |
