Claims
- 1. An antibiotic substance named chloropolysporin B and pharmaceutically acceptable salts thereof, which, as its sulfate, is characterized by the properties:
- (a) it takes the form of an amphoteric white powder, soluble in water;
- (b) specific rotation: [.alpha.].sup.25 -64.5.degree. (C=1.04, 0.1 N aqueous hydrochloric acid, sodium D-line);
- (c) elemental analysis:
- C, 48.33%; H, 5.05%; N, 5.48%; Cl, 5.11%; S, 1.00%;
- (d) on acid hydrolysis it yields:
- neutral saccharides: glucose, mannose and rhamnose; amino acids: 3-chloro-4-hydroxyphenylglycine and N-methyl-p-hydroxyphenylglycine;
- (e) ultraviolet absorption spectrum:
- as illustrated in FIG. 1 of the accompanying drawings, having an absorption maximum .lambda..sub.max at 280 nm (E.sub.1cm =51) in a 0.1 N solution of hydrochloric acid, the absorbence, E, being measured at a concentration of 1% w/v;
- (f) infrared absorption spectrum;
- the infrared absorption spectrum (.upsilon. cm.sup.-1) measured on a KBr disc is as shown in FIG. 2 of the accompanying drawings;
- (g) nuclear magnetic resonance spectrum:
- the nuclear magnetic resonance spectrum (.delta. ppm), measured at 270 MHz in deuterated dimethyl sulfoxide using tetramethylsilane as the internal standard, is as illustrated in FIG. 3 of the accompanying drawings;
- (h) solubility:
- soluble in water and methanol, sparingly soluble in acetone, and insoluble in ethyl acetate, chloroform and benzene;
- (i) color reactions:
- positive in Ninhydrin and Rydon-Smith reactions;
- (j) thin layer chromatography:
- Rf value=0.65, using a cellulose sheet (Eastman) as adsorbent and a 15:10:3:12 by volume mixture of butanol, pyridine, acetic acid and water as the developing solvent;
- (k) high voltage paper electrophoresis: using Toyo's filter paper No. 51A in a 0.1 M tris-hydrochloric acid buffer solution of pH 7.5 (3300 volt/60 cm, 1 hour); the migration distance (detected by bioautography with Bacillus subtilis PCI 219) from the origin to the cathode was 4 cm;
- (l) molecular formula:
- C.sub.83 H.sub.89 O.sub.34 N.sub.8 Cl.sub.3. 0.5H.sub.2 SO.sub.4. 10H.sub.2 O;
- (m) molecular weight:
- the molecular weight, measured by FAB-MS, was 1846 (MH.sup.+, 1847).
- 2. A pharmaceutical or veterinary composition comprising the antibiotic substance chloropolysporin B or a salt thereof in an antibiotic effective amount as defined in claim 1 in admixture with a pharmaceutical or veterinary carrier or diluent.
- 3. A method for the treatment or prophylaxis of bacterial infections by administering an antibiotic effective amount of the antibiotic substance chloropolysporin B or a salt thereof as defined in claim 1 to an animal.
- 4. A method of promoting the growth of a farm animal by the oral administration of an effective amount of the antibiotic substance chloropolysporin B or a salt thereof as defined in claim 1 to said animal.
- 5. An antibiotic composition comprising 0.1 to 1.0 grams of the chloropolysporin B or salt thereof of claim 1 in a pharmaceutical carrier or diluent.
- 6. A compound of the formula: ##STR2## wherein: R.sup.1 represents a ristosamine residue;
- R.sup.2 represents a mannose residue;
- R.sup.3 represents a glucose residue; and
- R.sup.4 represents a rhamnose residue;
- or a pharmaceutically acceptable salt thereof.
Priority Claims (1)
Number |
Date |
Country |
Kind |
60-2805 |
Jan 1985 |
JPX |
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Parent Case Info
This is a division of application Ser. No. 07/379,656 filed July 11, 1989 (issued as U.S. Pat. No. 4,918,054 on Apr. 17, 1990) which is a continuation of application Ser. No. 06/813,617 filed Dec. 26, 1985 (abandoned).
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4557933 |
Haneishi et al. |
Dec 1985 |
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Non-Patent Literature Citations (3)
Entry |
Okazaki et al., The Journal of Antibiotics, vol. 40, No. 7, pp. 917-923 (7/87). |
Takatsu et al., The Journal of Antibiotics, vol. 40, No. 7, pp. 924-945 (7/87). |
Takatsu et al., The Journal of Antibiotics, vol. 40, No. 7, pp. 946-952 (7/89). |
Divisions (1)
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Number |
Date |
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Parent |
379656 |
Jul 1989 |
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Continuations (1)
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Number |
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813617 |
Dec 1985 |
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