Antibodies, pharmaceutical compositions and uses thereof

Abstract
The present invention provides antibodies or the antigen-binding portion thereof, that bind to one or more carbohydrate antigens. Also disclosed herein are pharmaceutical pharmaceutical compositions and methods for the inhibition of cancer cells in a subject in need thereof. The pharmaceutical compositions comprise an antibody or an antigen-binding portion thereof and at least one pharmaceutically acceptable carrier.
Description
FIELD

The present invention relates to antibodies to tumor-associated carbohydrate antigens, including specific portions or variants specific for at least one tumor-associated carbohydrate antigen or fragment thereof, as well as nucleic acids encoding such antibodies, complementary nucleic acids, vectors, host cells and methods of making and using thereof, including therapeutic formulations and pharmaceutical compositions comprising the antibody. Further, methods are provided for administering antibodies to a subject in an amount effective to inhibit cancer cells.


BACKGROUND

Numerous surface carbohydrates are expressed in malignant tumor cells. For example, Globo H (Fuc α1-->2Galß1-->3GalNAcß1-->3Gal α1-->4Galß1-->4Glc) has been shown to be overexpressed on a variety of epithelial cancers and is associated with tumor aggressiveness and poor prognosis in breast cancer and small cell lung carcinoma. Previous studies have shown that Globo H and Stage-specific embryonic antigen 3 (SSEA3, also called Gb5) were observed on breast cancer cells and breast cancer stem cells (W W Chang et al. “Expression of Globo H and SSEA3 in breast cancer stem cells and the involvement of fucosyl transferases 1 and 2 in Globo H synthesis. PNAS, 105(33):11667-11672).


These findings support a rationale for the development of antibodies to tumor associated carbohydrate antigens, as there is still an unmet need for effective treatment and/or prevention for cancer. The present invention provides antibodies to tumor associated carbohydrate antigens to satisfy these and other needs.


SUMMARY OF THE INVENTION

The present invention provides for antibodies, or antigen-binding portions thereof, comprising a variable domain that bind to a carbohydrate antigen, conjugated versions of these antibodies, encoding or complementary nucleic acids, vectors, host cells, compositions, formulations, devices, transgenic animals, transgenic plants related thereto, and methods of making and using thereof, as described and enabled herein, in combination with what is known in the art. The antibody or antigen-binding portion thereof may have a dissociation constant (KD) of about 10E-7 M or less, about 10E-8 M or less, about 10E-9 M or less, about 10E-10 M or less, about 10E-11 M or less, or about 10E-12 M or less. The antibody or antigen-binding portion thereof may be humanized or chimeric.


In one embodiment, the present invention provides for an antibody, or an antigen-binding portion thereof, comprising a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 3


In another embodiment, the present invention provides for an antibody, or an antigen-binding portion thereof, comprising a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 4.


In yet another embodiment, the present invention provides for an antibody, or an antigen-binding portion thereof, comprising a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 3; and a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 4.


In a fourth embodiment, the present invention provides an antibody, or an antigen-binding portion thereof, comprises a heavy chain region, wherein the heavy chain region comprises three complementarity determining regions (CDRs), CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 5, 6 and 7, respectively. In an exemplary embodiment, the heavy chain further comprises a framework between a leader sequence and said CDR1 having an amino acid sequence about 80% to about 100% homologous to SEQ ID NO: 87. In another embodiment, the heavy chain further comprises a framework between said CDR2 and said CDR3 having an amino acid sequence about 80% to about 100% homologous to SEQ ID NO: 89. In yet another exemplary embodiment, the heavy chain further comprises a framework between said CDR1 and said CDR2 of the heavy chain having amino acid sequence about 80% to about 100% homologous to SEQ ID NO: 11, wherein the framework contains glycine at position 9 and the antibody or the antigen-binding portion thereof binds to a carbohydrate antigen, such as Globo H.


In a fifth embodiment, the present invention provides an antibody, or an antigen-binding portion thereof, comprises a light chain region, wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 8, 9 and 10, respectively. In an exemplary embodiment, the light chain further comprises a framework between a leader sequence and said CDR1 having an amino acid sequence about 80% to about 100% homologous to SEQ ID NO: 88. In another exemplary embodiment, the light chain further comprises a framework between said CDR2 and said CDR3 of the light chain, having an amino acid sequence about 80% to about 100% homologous to SEQ ID NO: 90. In yet another exemplary embodiment, the light chain further comprises a framework between said CDR1 and said CDR2 of the light chain having amino acid sequence about 80% to about 100% homologous to SEQ ID NO: 12, wherein the framework contains proline at position 12, and the antibody or the antigen-binding portion thereof binds to Globo H. In yet another exemplary embodiment, the light chain further comprises a framework between said CDR1 and said CDR2 of the light chain having amino acid sequence about 80% to about 100% homologous to SEQ ID NO: 12, wherein the framework contains tryptophan at position 13, and the antibody or the antigen-binding portion thereof binds to a carbohydrate antigen, such as Globo H.


In a sixth embodiment, the present invention provides an antibody, or an antigen-binding portion thereof, comprising a heavy chain region and a light chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 5, 6 and 7, respectively, and wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 8, 9 and 10, respectively.


In some embodiments, an antibody, or an antigen-binding portion thereof, comprising: a heavy chain region, wherein the heavy chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 5, 6 or 7 are provided. In other embodiments, an antibody, or an antigen-binding portion thereof, comprising a light chain region, wherein the light chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 8, 9 or 10 are provided.


The present invention is also directed to an antibody, or an antigen-binding portion thereof, comprising: a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 13.


The present invention is also directed to an antibody, or an antigen-binding portion thereof, comprising: a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 14.


The present invention is also directed to an antibody, or an antigen-binding portion thereof, comprising: a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 13; and a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 14.


An exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprises a heavy chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 15, 16 and 17, respectively. Another exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprises a light chain region, wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 18, 19 and 20, respectively.


Another exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprising a heavy chain region and a light chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 15, 16 and 17, respectively, and wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 18, 19 and 20, respectively.


In some embodiments, an antibody, or an antigen-binding portion thereof, comprising: a heavy chain region, wherein the heavy chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 15, 16 or 17 are provided. In other embodiments, an antibody, or an antigen-binding portion thereof, comprising a light chain region, wherein the light chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 18, 19 or 20 are provided.


One embodiment of the present invention is an antibody, or an antigen-binding portion thereof, comprising: a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 21.


Another embodiment of the present invention is an antibody, or an antigen-binding portion thereof, comprising: a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 22.


In yet another embodiment of the present invention is an antibody, or an antigen-binding portion thereof, comprising: a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 21; and a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 22.


An exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprises a heavy chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 23, 24 and 25, respectively. Another exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprises a light chain region, wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 26, 27 and 28, respectively.


Another exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprising a heavy chain region and a light chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 23, 24 and 25, respectively, and wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 26, 27 and 28, respectively.


In some embodiments, an antibody, or an antigen-binding portion thereof, comprising: a heavy chain region, wherein the heavy chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 23, 24 or 25 are provided. In other embodiments, an antibody, or an antigen-binding portion thereof, comprising a light chain region, wherein the light chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 26, 27 or 28 are provided.


The present invention also discloses an antibody, or an antigen-binding portion thereof; comprising: a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 29.


The present invention also discloses an antibody, or an antigen-binding portion thereof, comprising: a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 30.


The present invention also discloses an antibody, or an antigen-binding portion thereof; comprising: a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 29; and a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 30.


An exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprises a heavy chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 31, 32 and 33, respectively. Another exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprises a light chain region, wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 34, 35 and 36, respectively.


Another exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprising a heavy chain region and a light chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 31, 32 and 33, respectively, and wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 34, 35 and 36, respectively.


In some embodiments, an antibody, or an antigen-binding portion thereof, comprising: a heavy chain region, wherein the heavy chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 31, 32 or 33 are provided. In other embodiments, an antibody, or an antigen-binding portion thereof, comprising a light chain region, wherein the light chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 34, 35 or 36 are provided.


One embodiment of the present invention provides an antibody, or an antigen-binding portion thereof, comprising: a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 37.


Another embodiment of the present invention provides an antibody, or an antigen-binding portion thereof, comprising: a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 38.


Another embodiment of the present invention provides an antibody, or an antigen-binding portion thereof, comprising: a heavy chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 37; and a light chain variable domain comprising an amino acid sequence about 80% to about 100% homologous to the amino acid sequence shown in SEQ ID NO: 38.


An exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprises a heavy chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 39, 40 and 41, respectively. Another exemplary embodiment discloses an antibody, or an antigen-binding portion thereof, comprises a light chain region, wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 42, 43 and 44, respectively.


Another exemplary embodiment provides an antibody, or an antigen-binding portion thereof, comprising a heavy chain region and a light chain region, wherein the heavy chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 39, 40 and 41, respectively, and wherein the light chain region comprises three CDRs, CDR1, CDR2 and CDR3, having amino acid sequences about 80% to about 100% homologous to the amino acid sequences set forth in SEQ ID NOs: 42, 43 and 44, respectively.


In some embodiments, an antibody, or an antigen-binding portion thereof, comprising: a heavy chain region, wherein the heavy chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 39, 40 or 41 are provided. In other embodiments, an antibody, or an antigen-binding portion thereof, comprising a light chain region, wherein the light chain region comprises a CDR having an amino acid sequence about 80% to about 100% homologous to the amino acid sequence selected from SEQ ID NOs: 42, 43 or 44 are provided.


The present invention provides for a pharmaceutical composition comprising the antibody or antigen-binding portion thereof as described herein and at least one pharmaceutically acceptable carrier.


The present invention also provides for a method of inhibiting Globo H expressing cancer cells, comprising administering to a subject in need thereof an effective amount of the antibody or antigen-binding portion thereof described herein, wherein the Globo H expressing cancer cells are inhibited.


The present invention also provides for hybridoma clones designated as 2C2 (deposited under American Type Culture Collection (ATCC) Accession Number PTA-121138), 3D7 (deposited under ATCC Accession Number PTA-121310), 7A11 (deposited under ATCC Number PTA-121311), 2F8 (deposited under ATCC Accession Number PTA-121137) and 1E1 (deposited under ATCC Accession Number PTA-121312), and antibodies or antigen-binding portions produced therefrom.





BRIEF DESCRIPTION OF THE DRAWINGS


FIG. 1 is a linear plot showing the effect of PBS, Globo H-VK9 mAbs, Globo H-2C2 mAbs and Globo H-3D7 mAb on pancreatic cancer (HPAC) volume in mice.



FIG. 2 is a linear plot showing the effect of normal saline and different doses of Globo H-2C2 mAbs on breast cancer (MCF7) volume in mice.



FIG. 3A-3F are bar graphs showing the binding affinity of Globo H-2C2 mAb (FIG. 3A), Globo H-7A11 mAb (FIG. 3B), Globo H-3D7 mAb (FIG. 3C), Globo H-2F8 mAb (FIG. 3D), Globo H-1E1 mAb (FIG. 3E) and Globo H-VK9 mAb (FIG. 3F) with various carbohydrate antigens.





DETAILED DESCRIPTION OF THE INVENTION

As used herein, the articles “a” and “an” refer to one or more than one (i.e., at least one) of the grammatical object of the article. By way of example, “an element” means one element or more than one element.


An “effective amount,” as used herein, refers to a dose of the vaccine or pharmaceutical composition that is sufficient to reduce the symptoms and signs of cancer, such as weight loss, pain and palpable mass, which is detectable, either clinically as a palpable mass or radiologically through various imaging means. The term “effective amount” and “therapeutically effective amount” are used interchangeably.


The term “subject” can refer to a vertebrate having cancer or to a vertebrate deemed to be in need of cancer treatment. Subjects include all warm-blooded animals, such as mammals, such as a primate, and, more preferably, a human. Non-human primates are subjects as well. The term subject includes domesticated animals, such as cats, dogs, etc., livestock (for example, cattle, horses, pigs, sheep, goats, etc.) and laboratory animals (for example, mouse, rabbit, rat, gerbil, guinea pig, etc.). Thus, veterinary uses and medical formulations are contemplated herein.


All numbers herein are approximations and may be modified by “about.”


The present invention provides for pharmaceutical compositions and methods for the treatment or inhibition of cancer cells. The pharmaceutical compositions comprises antibodies that recognize carbohydrate antigen, including mouse monoclonal antibodies, humanized antibodies, chimeric antibodies, or antigen-binding portions of any of the foregoing. These antibodies (or antigen-binding portion thereof) can neutralize the carbohydrate antigen, and/or inhibit cancer cells. Therefore, the present antibodies or antigen-binding portion thereof can be used in the treatment or inhibition of cancer cells.


Antibodies of the present invention include any protein or peptide that comprise at least one complementarity determining region (CDR) of a heavy or light chain, or a ligand binding portion thereof, derived from an antibody produced by the hybridoma designated 2C2 (deposited under ATCC Accession No.: PTA-121138), the hybridoma designated 3D7 (deposited under ATCC Accession No.: PTA-121310), the hybridoma designated 7A11 (deposited under ATCC Accession No.: PTA-121311), the hybridoma designated 2F8 (deposited under ATCC Accession No.: PTA-121137), or the hybridoma designated 1E1 (deposited under ATCC Accession No.: PTA-121312) as described herein. Antibodies include antibody fragments, antibody variants, monoclonal antibodies, polyclonal antibodies, and recombinant antibodies and the like. Antibodies can be generated in mice, rabbits or humans.


Antibodies of the present invention also include chimerized or humanized monoclonal antibodies generated from antibodies of the present invention.


Thus, anti-cancer antibodies of the present invention include in combination with a heavy chain or light chain variable region, a heavy chain or light chain constant region, a framework region, or any portion thereof, of non-murine origin, preferably of human origin, which can be incorporated into an antibody of the present invention.


Antibodies of the present invention are capable of modulating, decreasing, antagonizing, mitigating, alleviating, blocking, inhibiting, abrogating and/or interfering with at least one Globo-H expressing cancer cell activity in vitro, in situ and/or in vivo.


The term “antibody” is further intended to encompass antibodies, digestion fragments, specified portions and variants thereof, including antibody mimetics or comprising portions of antibodies that mimic the structure and/or function of an anti-cancer antibody or specified fragment or portion thereof, including single chain antibodies and fragments thereof, each containing at least one CDR derived from an anti-cancer antibody of the present invention. Functional fragments include antigen-binding fragments that bind to a Globo-H expressing cancer cells. For example, antibody fragments capable of binding to Globo-H expression cancer cells or portions thereof, including, but not limited to Fab (e.g., by papain digestion), Fab′ (e.g., by pepsin digestion and partial reduction) and F(ab′)2 (e.g., by pepsin digestion), facb (e.g., by plasmin digestion), pFc′ (e.g., by pepsin or plasmin digestion), Fd (e.g., by pepsin digestion, partial reduction and reaggregation), Fv or scFv (e.g., by molecular biology techniques) fragments, are encompassed by the invention (see, e.g., Colligan, Immunology, supra).


As used herein, 2C2 refers to the hybridoma clone or the antibodies generated by the corresponding hybridoma clone.


An antigen-binding portion of an antibody may include a portion of an antibody that specifically binds to a carbohydrate antigen (e.g., Globo H, SSEA-3 or SSEA-4).


The humanized antibody of the present invention is an antibody from a non-human species where the amino acid sequence in the non-antigen binding regions (and/or the antigen-binding regions) has been altered so that the antibody more closely resembles a human antibody while retaining its original binding ability.


Humanized antibodies can be generated by replacing sequences of the variable region that are not directly involved in antigen binding with equivalent sequences from human variable regions. Those methods include isolating, manipulating, and expressing the nucleic acid sequences that encode all or part of variable regions from at least one of a heavy or light chain. Sources of such nucleic acid are well known to those skilled in the art. The recombinant DNA encoding the humanized antibody, or fragment thereof, can then be cloned into an appropriate expression vector.


An antibody light or heavy chain variable region comprises a framework region interrupted by three hypervariable regions, referred to as CDRs. In one embodiment, humanized antibodies are antibody molecules from non-human species having one, two or all CDRs from the non-human species and one, two or all three framework regions from a human immunoglobulin molecule.


According to one aspect of the invention, the location of the CDRs and framework residues are determined by methods disclosed in Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242. According to another aspect of the invention, the antibody or the antigen-binding portion thereof may have the following structure:


Leader Sequence—FW1-CDR1-FW2-CDR2-FW3-CDR3-


in which the framework regions FW1, FW2, FW3 and the CDRs CDR1, CDR2, CDR3 have amino acid sequences disclosed in Table 1.


The humanized antibodies of the present invention can be produced by methods well known in the art. For example, once non-human (e.g., murine) antibodies are obtained, variable regions can be sequenced, and the location of the CDRs and framework residues determined. Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242. Chothia, C. et al. (1987) J. Mol. Biol., 196:901-917. DNA encoding the light and heavy chain variable regions can, optionally, be ligated to corresponding constant regions and then subcloned into an appropriate expression vector. CDR-grafted antibody molecules can be produced by CDR-grafting or CDR substitution. One, two, or all CDRs of an immunoglobulin chain can be replaced. For example, all of the CDRs of a particular antibody may be from at least a portion of a non-human animal (e.g., mouse such as CDRs shown in Table 1) or only some of the CDRs may be replaced. It is only necessary to keep the CDRs required for binding of the antibody to a predetermined carbohydrate antigen (e.g., Globo H). Morrison, S. L., 1985, Science, 229:1202-1207. Oi et al., 1986, BioTechniques, 4:214. U.S. Pat. Nos. 5,585,089; 5,225,539; 5,693,761 and 5,693,762. EP 519596. Jones et al., 1986, Nature, 321:552-525. Verhoeyan et al., 1988, Science, 239:1534. Beidler et al., 1988, J. Immunol., 141:4053-4060.


Also encompassed by the present invention are antibodies or antigen-binding portions thereof comprising one or two variable regions as disclosed herein, with the other regions replaced by sequences from at least one different species including, but not limited to, human, rabbits, sheep, dogs, cats, cows, horses, goats, pigs, monkeys, apes, gorillas, chimpanzees, ducks, geese, chickens, amphibians, reptiles and other animals.


A chimeric antibody is a molecule in which different portions are derived from different animal species. For example, an antibody may contain a variable region derived from a murine mAb and a human immunoglobulin constant region. Chimeric antibodies can be produced by recombinant DNA techniques. Morrison, et al., Proc Natl Acad Sci, 81:6851-6855 (1984). For example, a gene encoding a murine (or other species) antibody molecule is digested with restriction enzymes to remove the region encoding the murine Fc, and the equivalent portion of a gene encoding a human Fc constant region is then substituted into the recombinant DNA molecule. Chimeric antibodies can also be created by recombinant DNA techniques where DNA encoding murine V regions can be ligated to DNA encoding the human constant regions. Better et al., Science, 1988, 240:1041-1043. Liu et al. PNAS, 1987 84:3439-3443. Liu et al., J. Immunol., 1987, 139:3521-3526. Sun et al. PNAS, 1987, 84:214-218. Nishimura et al., Canc. Res., 1987, 47:999-1005. Wood et al. Nature, 1985, 314:446-449. Shaw et al., J. Natl. Cancer Inst., 1988, 80:1553-1559. International Patent Publication Nos. WO1987002671 and WO 86/01533. European Patent Application Nos. 184,187; 171,496; 125,023; and 173,494. U.S. Pat. No. 4,816,567.


The antibodies can be full-length or can comprise a fragment (or fragments) of the antibody having an antigen-binding portion, including, but not limited to, Fab, F(ab′)2, Fab′, F(ab)′, Fv, single chain Fv (scFv), bivalent scFv (bi-scFv), trivalent scFv (tri-scFv), Fd, dAb fragment (e.g., Ward et al., Nature, 341:544-546 (1989)), an isolated CDR, diabodies, triabodies, tetrabodies, linear antibodies, single-chain antibody molecules, and multispecific antibodies formed from antibody fragments. Single chain antibodies produced by joining antibody fragments using recombinant methods, or a synthetic linker, are also encompassed by the present invention. Bird et al. Science, 1988, 242:423-426. Huston et al., Proc. Natl. Acad. Sci. USA, 1988, 85:5879-5883.


The antibodies or antigen-binding portions thereof of the present invention may be monospecific, bi-specific or multispecific. Multispecific or bi-specific antibodies or fragments thereof may be specific for different epitopes of one target carbohydrate (e.g., Globo H) or may contain antigen-binding domains specific for more than one target carbohydrate (e.g., antigen-binding domains specific for Globo H, SSEA-3 and SSEA-4). In one embodiment, a multispecific antibody or antigen-binding portion thereof comprises at least two different variable domains, wherein each variable domain is capable of specifically binding to a separate carbohydrate antigen or to a different epitope on the same carbohydrate antigen. Tutt et al., 1991, J. Immunol. 147:60-69. Kufer et al., 2004, Trends Biotechnol. 22:238-244. The present antibodies can be linked to or co-expressed with another functional molecule, e.g., another peptide or protein. For example, an antibody or fragment thereof can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovalent association or otherwise) to one or more other molecular entities, such as another antibody or antibody fragment to produce a bi-specific or a multispecific antibody with a second binding specificity.


All antibody isotypes are encompassed by the present invention, including IgG (e.g., IgG1, IgG2, IgG3, IgG4), IgM, IgA (IgA1, IgA2), IgD or IgE (all classes and subclasses are encompassed by the present invention). The antibodies or antigen-binding portions thereof may be mammalian (e.g., mouse, human) antibodies or antigen-binding portions thereof. The light chains of the antibody may be of kappa or lambda type.


The variable regions of the present antibodies or antigen-binding portions thereof can be from a non-human or human source. The framework of the present antibodies or antigen-binding portions thereof can be human, humanized, non-human (e.g., a murine framework modified to decrease antigenicity in humans), or a synthetic framework (e.g., a consensus sequence).


In one embodiment, the present antibodies, or antigen-binding portions thereof, comprise at least one heavy chain variable region and/or at least one light chain variable region.


The present antibodies or antigen-binding portions thereof specifically bind to Globo H with a dissociation constant (KD) of less than about 10E-7 M, less than about 10E-8 M, less than about 10E-9 M, less than about 10E-10 M, less than about 10E-11 M, or less than about 10E-12 M. In one embodiment, the antibody or the antibody binding portion thereof has a dissociation constant (KD) of 1˜10×10E-9 or less. In another embodiment, the Kd is determined by surface plasmon resonance.


Antibodies with a variable heavy chain region and a variable light chain region that are at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to the variable heavy chain region and variable light chain region of the antibody produced by clone 2C2, and can also bind to a carbohydrate antigen (e.g. Globo H). Homology can be present at either the amino acid or nucleotide sequence level.


In some embodiments, the antibodies or antigen-binding portions thereof include, for example, the variable heavy chains and/or variable light chains of the antibodies produced by hybridoma 2C2, hybridoma 3D7, hybridoma 7A11, hybridoma 2F8 and hybridoma 1E1, are shown in Table 1.


In related embodiments, the antibodies or antigen-binding portions thereof include, for example, the CDRs of the variable heavy chains and/or the CDRs of the variable light chains of the antibodies produced from hybridoma 2C2, hybridoma 3D7, hybridoma 7A11, hybridoma 2F8 and hybridoma 1E1. The CDRs and frameworks of the variable heavy chains and the variable light chains from these hybridoma clones are shown in Table 1.









TABLE 1







SEQ ID NO. 1-90










Hybridoma


SEQ


Clones
Chain Region
Sequence
ID No.





2C2
Heavy Chain
Nucleic acid Sequence
 1



Variable Region
TCTGGCCCTGGGATATTGCAGCCCTCCCAGACCCTCAGTCTGAC




(Vh)
TTGTTCTTTCTCTGGATTTTCACTGTACACTTTTGATATGGGTG





TAGGCTGGATTCGTCAGCCTTCAGGGAAGGGTCTGGAGTGGCTG





GCACACATTTGGTGGGATGATGATAAGTACTATAACCCAGCCCT





GAAGAGTCGGCTCACAGTCTCCAAGGATACCTCCAAAAACCAGG





TCTTCCTCAAGATCCCCAATGTGGACACTGCAGATAGTGCCACA





TACTACTGTGCTCGAGTAAGGGGCCTCCATGATTATTACTACTG





GTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCT






2C2
Light Chain
Nucleic acid Sequence
 2



Variable Region
GCATCTCCAGGGGAGAAGGTCACAATGACTTGCAGGGCCAGTTC




(VL)
AAGTGTAAGTTACATGCACTGGTACCAGCAGAAGCCAGGATCCT





CCCCCAAACCCTGGATTTATGCCACATCCAACCTGGCGTCTGGA





GTCCCTGCTCGCTTCAGTGGCAGTGGGTCTGGGACCTCTTACTC





TCTCACAATCAGCAGAGTGGAGGCTGAAGATGCTGCCACTTATT





TCTGCCAGCAGTGGAGTCGAAACCCATTCACGTTCGGCTCGGGG





ACAAAGTTGGAAATAAGA






2C2
Heavy Chain
Amino Acid Sequence
 3



Varible Region
SGPG ILQPSQTLSL TCSFSGFSLY TFDMGVGWIR




(Vh)
QPSGKGLEWL AHIWWDDDKY YNPALKSRLT VSKDTSKNQV





FLKIPNVDTA DSATYYCARV RGLHDYYYWF AYWGQGTLVT





VS






2C2
Light Chain
Amino Acid Sequence
 4



(VL)
ASPGEKVT MTCRASSSVS YMHWYQQKPG SSPKPWIYAT





SNLASGVPAR FSGSGSGTSY SLTISRVEAE DAATYFVQQW





SRNPFTFGSG TKLEIR






2C2
Heavy Chain
Amino Acid Sequence
 5



CDR1
YTFDMGVG






2C2
Heavy Chain
Amino Acid Sequence
 6



CDR2
HIWWDDDKYYNPALKS






2C2
Heavy Chain
Amino Acid Sequence
 7



CDR3
VRGLHDYYYWFAY






2C2
Light Chain
Amino Acid Sequence
 8



CDR1
RASSSVSYMH






2C2
Light Chain
Amino Acid Sequence
 9



CDR2
ATSNLAS






2C2
Light Chain
Amino Acid Sequence
10



CDR3
QQWSRNPFT






2C2
Heavy Chain
Amino Acid Sequence
11



Frame work 2
WIRQPSGKGLEWLA






2C2
Light Chain
Amino Acid Sequence
12



Frame work 2
WYQQKPGSSPKPWIY






3D7
Heacy Chain
Amino Acid Sequence
13



Variable Region
SGPGILQPSQTLSLTCSFSGFSLYTFDMGVGWIRQPSGKGLEW




(Vh)
LAHIWWDDDKYYNPALKSRLTVSKDTSKNQVFLKIPNVDTADS





ATYYCARVRGLHDYYYWFAYWGQGTLVTVS






3D7
Light Chain
Amino Acid Sequence
14



Variable Region
ASPGEKVTMTCRASSSVSYMHWYQQKPGSSPKPWIYATSNLAS




(VL)
GVPARFSGSGSGTSYSLTISRVEAEDAATYFCQQWSRNPFTFG





SGTKLEIR






3D7
Heavy Chain
Amino Acid Sequence
15



CDR1
YTFDMGVG






3D7
Heavy Chain
Amino Acid Sequence
16



CDR2
HIWWDDDKYYNPALKS






3D7
Heavy Chain
Amino Acid Sequence
17



CDR3
VRGLHDYYYWFAY






3D7
Light Chain
Amino Acid Sequence
18



CDR1
RASSSVSYMH






3D7
Light Chain
Amino Acid Sequence
19



CDR2
ATSNALS






3D7
Light Chain
Amino Acid Sequence
20



CDR3
QQWSRNPFT






7A11
Heavy Chain
Amino Acid Sequence
21



Variable Regin
SGPGILQPSQTLSLTCSFSGFSLYTFDMGVGWIRQPSGKGLEW




(Vh)
LAQIWWDDDKYYNPGLKSRLTISKDTSKNQVFLKIPNVDTADS





ATYYCARIRGLRDYYYWFAYWGQGTLVTVS






7A11
Light Chain
Amino Acid Sequence
22



Variable Region
ASPGEKVTMTCRASSSVSYMHWYQQKPGSSPKPWIYATSNLAS




(VL)
GVPARFSGSGSGTSYSLTISRVEAEDAATYFCQQWSRNPFTFG





SGTKLEIR






7A11
Heavy Chain
Amino Acid Sequence
23



CDR1
YTFDMGVG






7A11
Heavy Chain
Amino Acid Sequence
24



CDR2
QIWWDDDKYYNPGLKS






7A11
Heavy Chain
Amino Acid Sequence
25



CDR3
IRGLRDYYYWFAY






7A11
Light Chain
Amino Acid Sequence
26



CDR1
RASSSVSYMH






7A11
Light Chain
Amino Acid Sequence
27



CDR2
ATSNLAS






7A11
Light Chain
Amino Acid Sequence
28



CDR3
QQWSRNPFT






2F8
Heavy Chain
Amino Acid Sequence
29



Variable Region
SGPGILQPSQTLSLTCSFSGFSLSTFGLGVGWIRQPSGKGLEW




(Vh)
LAHIWWDDDKSYNPALKSRLTISKDTSKNQVFLMIANVDTADT





ATYYCARIGPKWSNYYYCDYWGQGTTLTVS






2F8
Light Chain
Amino Acid Sequence
30



Variable Region
ASPGEKVTMTCRASSSVSYMHWYQQKPGSSPKPYIYATSNLSS




(VL)
GVPARFSGSGSGTSYSLTISRVEAEDAATYYCQQWSSNPFTFG





SGTKLEIK






2F8
Heavy Chain
Amino Acid Sequence
31



CDR1
STFGLGVG






2F8
Heavy Chain
Amino Acid Sequence
32



CDR2
HIWWDDDKSYNPALKS






2F8
Heavy Chain
Amino Acid Sequence
33



CDR3
IGPKWNSYYYYCDY






2F8
Light Chain
Amino Acid Sequence
34



CDR1
RASSSVSYMH






2F8
Light Chain
Amino Acid Sequence
35



CDR2
ATSNLSS






2F8
Light Chain
Amino Acid Sequence
36



CDR3
QQWSSNPFT






1E1
Heavy Chain
Amino Acid Sequence
37



Variable Region
SGPGILQPSQTLSLTCSFSGFSLSTFGLGVGWIRQPSGKGLEW




(Vh)
LAHIWWDDDSKYNPALKSQLTISKDTSKNQVLLKIANVDTADT





ATYYCARIGPKWSNYYYYCDYWGQGTTLTVS






1E1
Light Chain
Amino Acid Sequence
38



Variable Region
ASPGEKVTMTCRASSSVSYNHWYQQKPGSSPKPYIYATSNLSS




(VL)
GVPARFSGSGSGTSYSLTISRVEAEDAATYYCQQWSSNPFTFG





SGTKLEIK






1E1
Heavy Chain
Amino Acid Sequence
39



CDR1
STFGLGVG






1E1
Heavy Chain
Amino Acid Sequence
40



CDR2
HIWWDDDKSYNPALKS






1E1
Heavy Chain
Amino Acid Sequence
41



CDR3
IGPKWNSYYYYCDY






1E1
Light Chain
Amino Acid Sequence
42



CDR1
RASSSVSYMH






1E1
Light Chain
Amino Acid Sequence
43



CDR2
ATSNLSS






1E1
Light Chain
Amino Acid Sequence
44



CDR3
QQWSSNPFT






2C2
Heavy Chain
Amino Acid Sequence
45



CDR1
TACACTTTTGATATGGGTGTAGGC






2C2
Heavy Chain
Nucleic Acid Sequence
46



CDR2
CACATTTGGTGGGATGATGATAAGTACTATAACCCAGCCCTGA





AGAGT






2C2
Heavy Chain
Nucleic Acid Sequence
47



CDR3
GTAAGGGGCCTCCATGATTATTACTACTGGTTTTGCTTAC






2C2
Light Chain
Nucleic Acid Sequence
48



CDR1
AGGGCCAGTTCAAGTGTAAGTTACATGCAC






2C2
Light Chain
Nucleic Acid Sequence
49



CDR2
GCCACATCCAACCTGGCGTCT






2C2
Light Chain
Nucleic Acid Sequence
50



CDR3
CAGCAGTGGAGTCGAAACCCATTCACG






3D7
Heavy Chain
Nucleic Acid Sequence
51



Variable Region
TCTGGCCCTGGGATATTGCAGCCCTCCCAGACCCTCAGTCTGA




(Vh)
CTTGTTCTTTCTCTGGATTTTCACTGTACACTTTTGATATGGG





TGTAGGCTGGATTCGTCAGCCTTCAGGGAAGGGTCTGGAGTGG





CTGGCACACATTTGGTGGGATGATGATAAGTACTATAACCCAG





CCCTGAAGAGTCGGCTCACAGTCTCCAAGGATACCTCCAAAAA





CCAGGTCTTCCTCAAGATCCCCAATGTGGACACTGCAGATAGT





GCCACATACTACTGTGCTCGAGTAAGGGGCCTCCATGATTATT





ACTACTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGT





CTCT






3D7
Light Chain
Nucleic Acid Sequence
52



Variable Region
GCATCTCCAGGGGAGAAGGTCACAATGACTTGCAGGGCCAGTT




(VL)
CAAGTGTAAGTTACATGCACTGGTACCAGCAGAAGCCAGGATC





CTCCCCCAAACCCTGGATTTATGCCACATCCAACCTGGCGTCT





GGAGTCCCTGCTCGCTTCAGTGGCAGTGGGTCTGGGACCTCTT





ACTCTCTCACAATCAGCAGAGTGGAGGCTGAAGATGCTGCCAC





TTATTTCTGCCAGCAGTGGAGTCGAAACCCATTCACGTTCGGC





TCGGGGACAAAGTTGGAAATAAGA






3D7
Heavy Chain
Nucleic Acid Sequence
53



CDR1
TACACTTTTGATATGGGTGTAGGC






3D7
Heavy Chain
Nucleic Acid Sequence
54



CDR2
CACATTTGGTGGGATGATGATAAGTACTATAACCCAGCCCTGA





AGAGT






3D7
Heavy Chain
Nucleic Acid Sequence
55



CDR3
GTAAGGGGCCTCCATGATTATTACTACTGGTTTGCTTAC






3D7
Light Chain
Nucleic Acid Sequence
56



CDR1
AGGGCCAGTTCAAGTGTAAGTTACATGCAC






3D7
Light Chain
Nucleic Acid Sequence
57



CDR2
GCCACATCCAACCTGGCGTCT






3D7
Light Chain
Nucleic Acid Sequence
58



CDR3
CAGCAGTGGAGTCGAAACCCATTCACG






7A11
Heavy Chain
Nucleic Acid Sequence
59



Variable Region
TCTGGCCCTGGGATATTGCAGCCCTCCCAGACCCTCAGTCTGA




(Vh)
CTTGTTCTTTCTCTGGATTTTCACTGTACACTTTTGATATGGG





TGTAGGCTGGATTCGTCAGCCTTCAGGGAAGGGTCTGGAGTGG





CTGGCACAAATTTGGTGGGATGATGATAAGTACTATAACCCAG





GCCTGAAGAGTCGGCTCACAATCTCCAAGGATACCTCCAAAAA





CCAGGTATTCCTCAAGATCCCCAATGTGGACACTGCAGATAGT





GCCACATACTACTGTGCTCGAATAAGGGGCCTCCGTGATTATT





ACTACTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGT





CTCT






7A11
Light Chain
Nucleic Acid Sequence
60



Variable Region
GCATCTCCAGGGGAGAAGGTCACAATGACTTGCAGGGCCAGCT




(VL)
CAAGTGTAAGTTACATGCACTGGTACCAGCAGAAGCCAGGATC





CTCCCCCAAACCCTGGATTTATGCCACATCCAACCTGGCTTCT





GGAGTCCCTGCTCGCTTCAGTGGCAGTGGGTCTGGGACCTCTT





ACTCTCTCACAATCAGCAGAGTGGAGGCTGAAGATGCTGCCAC





TTATTTCTGCCAGCAGTGGAGTCGAAACCCATTCACGTTCGGC





TCGGGGACAAAGTTGGAAATAAGA






7A11
Heavy Chain
Nucleic Acid Sequence
61



CDR1
TACACTTTTGATATGGGTGTAGGC






7A11
Heavy Chain
Nucleic Acid Sequence
62



CDR2
CAAATTTGGTGGGATGATGATAAGTACTATAACCCAGGCCTGA





AGAGT






7A11
Heavy Chain
Nucleic Acid Sequence
63



CDR3
ATAAGGGGCCTCCGTGATTATTACTACTGGTTTGCTTAC






7A11
Light Chain
Nucleic Acid Sequence
64



CDR1
AGGGCCAGCTCAAGTGTAAGTTACATGCAC






7A11
Light Chain
Nucleic Acid Sequence
65



CDR2
GCCACATCCAACCTGGCTTCT






7A11
Light Chain
Nucleic Acid Sequence
66



CDR3
CAGCAGTGGAGTCGAAACCCATTCACG






2F8
Heavy Chain
Nucleic Acid Sequence
67



Variable Region
TCTGGCCCTGGGATATTGCAGCCCTCCCAGACCCTCAGTCTGA




(Vh)
CTTGTTCTTTCTCTGGGTTTTCGCTGAGCACTTTTGGTTTGGG





TGTAGGCTGGATTCGTCAGCCTTCAGGGAAGGGTCTGGAGTGG





CTGGCACACATTTGGTGGGATGATGATAAGTCCTATAACCCAG





CCCTGAAGAGTCGGCTCACAATCTCCAAGGATACCTCCAAAAA





CCAGGTCTTCCTCATGATCGCCAATGTGGACACTGCAGATACT





GCCACATACTACTGTGCTCGAATAGGCCCGAAATGGAGCAACT





ACTACTACTACTGTGACTACTGGGGCCAAGGCACCACTCTCAC





AGTCTCC






2F8
Light Chain
Nucleic Acid Sequence
68



Variable Region
GCATCTCCAGGGGAGAAGGTCACAATGACTTGCAGGGCCAGCT




(VL)
CAAGTGTTAGTTACATGCACTGGTACCAGCAGAAGCCAGGATC





CTCCCCCAAACCCTACATTTATGCCACATCCAACCTGTCTTCT





GGAGTCCCTGCTCGCTTCAGTGGCAGTGGGTCTGGGACCTCTT





ACTCTCTCACAATCAGCAGAGTGGAGGCTGAAGATGCTGCCAC





TTATTACTGCCAGCAGTGGAGTAGTAACCCCTTCACGTTCGGC





TCGGGGACAAAGTTGGAAATAAAA






2F8
Heavy Chain
Nucleic Acid Sequence
60



CDR1
AGCACTTTTGGTTTGGGTGTAGGC






2F8
Heavy Chain
Nucleic Acid Sequence
70



CDR2
CACATTTGGTGGGATGATGATAAGTCCTATAACCCAGCCCTGA





AGAGT






2F8
Heavy Chain
Nucleic Acid Sequence
71



CDR3
ATAGGCCCGAAATGGAGCAACTACTACTACTACTGTGACTAC






2F8
Light Chain
Nucleic Acid Sequence
72



CDR1
AGGGCCAGCTCAAGTGTTAGTTACATGCAC






2F8
Light Chain
Nucleic Acid Sequence
73



CDR2
GCCACATCCAACCTGTCTTCT






2F8
Light Chain
Nucleic Acid Sequence
74



CDR3
CAGCAGTGGAGTAGTAACCCCTTCACG






1E1
Heavy Chain
Nucleic Acid Sequence
75



Variable Region
TCTGGCCCTGGGATATTGCAGCCCTCCCAGACCCTCAGTCTGA




(Vh)
CTTGTTCTTTCTCTGGGTTTTCGCTGAGCACTTTTGGTTTGGG





TGTAGGCTGGATTCGTCAGCCTTCAGGGAAGGGTCTGGAGTGG





CTGGCACACATTTGGTGGGATGATGATAAGTCCTATAACCCAG





CCCTGAAGAGTCAGCTCACAATCTCCAAGGATACCTCCAAAAA





CCAGGTACTCCTCAAGATCGCCAATGTGGACACTGCAGATACT





GCCACATACTACTGTGCTCGAATAGGCCCGAAATGGAGCAACT





ACTACTACTACTGTGACTACTGGGGCCAAGGCACCACTCTCAC





AGTCTCC






1E1
Light Chain
Nucleic Acid Sequence
76



Variable Region
GCATCTCCAGGGGAGAAGGTCACAATGACTTGCAGGGCCAGCT




(VL)
CAAGTGTTAGTTACATGCACTGGTACCAGCAGAAGCCAGGATC





CTCCCCCAAACCCTACATTTATGCCACATCCAACCTGTCTTCT





GGAGTCCCTGCTCGCTTCAGTGGCAGTGGGTCTGGGACCTCTT





ACTCTCTCACAATCAGCAGAGTGGAGGCTGAAGATGCTGCCAC





TTATTACTGCCAGCAGTGGAGTAGTAACCCCTTCACGTTCGGC





TCGGGGACAAAGTTGGAAATAAAA






1E1
Heavy Chain
Nucleic Acid Sequence
77



CDR1
AGCACTTTTGGTTTGGGTGTAGGC






1E1
Heavy Chain
Nucleic Acid Sequence
78



CDR2
CACATTTGGTGGGATGATGATAAGTCCTATAACCCAGCCCTGA





AGAGT






1E1
Heavy Chain
Nucleic Acid Sequence
79



CDR3
ATAGGCCCGAAATGGAGCAACTACTACTACTACTGTGACTAC






1E1
Light Chain
Nucleic Acid Sequence
80



CDR1
AGGGCCAGCTCAAGTGTTAGTTACATGCAC






1E1
Light Chain
Nucleic Acid Sequence
81



CDR2
GCCACATCCAACCTGTCTTCT






1E1
Light Chain
Nucleic Acid Sequence
82



CDR3
CAGCAGTGGAGTAGTAACCCCTTCACG






2C2
Heavy Chain
Amino Acid Sequence
83



Frame work 1
SGPGILQPSQTLSLTCSFSGFSL






2C2
Light Chain
Amino Acid Sequence
84



Frame work 1
ASPGEKVTMTC






2C2
Heavy Chain
Amino Acid Sequence
85



Frame work 3
RLTVSKDTSKNQVFLKIPNVDTADSATYYCAR






2C2
Light Chain
Amino Acid Sequence
86



Frame work 3
GVPARSGSGSGTSYSLTISRVEAEDAATYFC






2C2
Heavy Chain
Amino Acid Sequence of Humanized Antibody
87



Frame work 1
SGPTLVKPTQTLTLTCTFSGFSL






2C2
Light Chain
Amino Acid Sequence of Humanized Antibody
88



Frame work 1
LSPGERATLSC






2C2
Heavy Chain
Amino Acid Sequence of Humanized Antibody
89



Frame work 3
RLTISKDTSKNQVVLTMTNMDPVDTATYYCAR






2C2
Light Chain
Amino Acid Sequence of Humanized Antibody
90



Frame work 3
GVPSRFSGSGSGTDFTFTISSLQPEDIATYYC









The invention also encompasses a nucleic acid encoding the present antibody or antigen-binding portion thereof that specifically binds to a carbohydrate antigen. In one embodiment, the carbohydrate antigen is Globo H. In another embodiment, the carbohydrate antigen is SSEA-3. In yet another embodiment, the carbohydrate antigen is SSEA-4. The nucleic acid may be expressed in a cell to produce the present antibody or antigen-binding portion thereof.


In certain embodiments, the antibodies or antigen-binding portions thereof include a variable heavy chain region comprising an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to any of the following:

    • a) SEQ ID NO: 3 (Hybridoma 2C2);
    • b) SEQ ID NO: 13 (Hybridoma 3D7);
    • c) SEQ ID NO: 21 (Hybridoma 7A11);
    • d) SEQ ID NO: 29 (Hybridoma 2F8); or
    • e) SEQ ID NO: 37 (Hybridoma 1E1).


In certain embodiments, the antibodies or antigen-binding portions thereof include a variable light chain region comprising an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to any of the following:

    • f) SEQ ID NO: 4 (Hybridoma 2C2);
    • g) SEQ ID NO: 14 (Hybridoma 3D7);
    • h) SEQ ID NO: 22 (Hybridoma 7A11);
    • i) SEQ ID NO: 30 (Hybridoma 2F8); or
    • j) SEQ ID NO: 38 (Hybridoma 1E1).


In certain embodiments, a variable heavy chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 3, and a variable light chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 4.


In certain embodiments, a variable heavy chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 13, and a variable light chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 14.


In certain embodiments, a variable heavy chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 21, and a variable light chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 22.


In certain embodiments, a variable heavy chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 29, and a variable light chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 30.


In certain embodiments, a variable heavy chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 37, and a variable light chain region of the antibodies or antigen-binding portions thereof comprises an amino acid sequence that is at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or about 100% homologous to SEQ ID NO: 38.


A variable heavy chain region of the antibodies or antigen-binding portions thereof can comprise one, two three or more CDRs comprise amino acid sequences that are at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to any of the following:

    • k) CDRs of the variable heavy chain region of an antibody produced by hybridoma 2C2 (SEQ ID NOs: 5, 6 and 7);
    • l) CDRs of the variable heavy chain region of an antibody produced by hybridoma 3D7 (SEQ ID NOs: 15, 16 and 17);
    • m) CDRs of the variable heavy chain region of an antibody produced by hybridoma 7A11 (SEQ ID NOs: 23, 24 and 25);
    • n) CDRs of the variable heavy chain region of an antibody produced by hybridoma 2F8 (SEQ ID NOs: 31, 32 and 33); or
    • o) CDRs of the variable heavy chain region of an antibody produced by hybridoma 1E1 (SEQ ID No: 39, 40 and 41).


A variable light chain region of the antibodies or antigen-binding portions thereof can comprise one, two three or more CDRs comprise amino acid sequences that are at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to any one of the following:

    • p) CDRs of the variable light chain region of an antibody produced by hybridoma 2C2 (SEQ ID NOs: 8, 9 and 10);
    • q) CDRs of the variable light chain region of an antibody produced by hybridoma 3D7 (SEQ ID NOs: 18, 19 and 20);
    • r) CDRs of the variable light chain region of an antibody produced by hybridoma 7A11 (SEQ ID NOs: 26, 27 and 28);
    • s) CDRs of the variable light chain region of an antibody produced by hybridoma 2F8 (SEQ ID NOs: 34, 35 and 36); or
    • t) CDRs of the variable light chain region of an antibody produced by hybridoma 1E1 (SEQ ID NOs: 42, 43 and 44).


A variable heavy chain region of the antibodies or antigen-binding portions thereof can comprise one, two three or more CDRs comprise amino acid sequences that are at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to CDRs of the variable heavy chain region of an antibody produced by hybridoma 2C2 (SEQ ID NOs: 5, 6, 7) or CDRs of the variable heavy chain region of an antibody produced by hybridoma 3D7 (SEQ ID NOs: 15, 16 and 17), and a variable light chain region of the antibodies or antigen-binding portions thereof can comprise one, two three or more CDRs comprise amino acid sequences that are at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to CDRs of the variable light chain region of an antibody produced by hybridoma 2C2 (SEQ ID NOs: 8, 9 and 10) or CDRs of the variable light chain region of an antibody produced by hybridoma 3D7 (SEQ ID NOs: 18, 19 and 20).


In one embodiment, the antibodies or antigen-binding portions thereof further comprises a framework at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to SEQ ID NO: 83 (Heavy Chain Framework 1 of 2C2 antibody) or SED ID NO: 87 (Heavy Chain Framework 1 of Humanized 2C2 Antibody, see Table 1), wherein the framework is between a leader sequence and CDR1 of a variable heavy chain region of the antibody produced by hybridoma 2C2. In another embodiment, the antibodies or antigen-binding portions thereof further comprises a framework at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to SEQ ID NO: 84 (Light Chain Framework 1 of 2C2 antibody) or SED ID NO:88 (Light Chain Framework 1 of Humanized 2C2 Antibody, see Table 1) and said framework is between a leader sequence and CDR1 of the variable light chain region of the antibody produced by hybridoma 2C2.


In one embodiment, the antibodies or antigen-binding portions thereof further comprises a framework between CDR1 and CDR2 of the variable heavy chain region of the antibody produced by hybridoma 2C2, wherein the framework is at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to SEQ ID NO: 11 (Heavy Chain Framework 2 in Table 1). In an exemplary embodiment, the framework between CDR1 and CDR2 of a variable heavy chain region having amino acid sequence about 80% to about 100% homologous to SEQ ID NO: 11, contains glycine at position 9. The position of the amino acid of SEQ ID NO: 11 is illustrated below:















Amino Acid






















W*
I
R
Q
P
S
G
K
G
L
E
W
L
A**

























Position
1
2
3
4
5
6
7
8
9
10
11
12
13
14


No.





*The amino acid at position 1 of FW2 (W) is the residue adjacent to CDR 1.


**The amino acid at position 14 of FW2 (A) is the residue adjacent to CDR2.






In another embodiment, the antibodies or antigen-binding portions thereof further comprises a framework between CDR1 and CDR2 of the variable light chain region of an antibody produced by hybridoma 2C2, wherein the framework is at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to SEQ ID NO: 12 (Light Chain Framework 2 in Table 1). In an exemplary embodiment, the framework between CDR1 and CDR2 of a variable light chain region of the antibody produced by hybridoma 2C2 contains proline at position 12. In another exemplary embodiment, the framework between CDR1 and CDR2 of a variable light chain region of the antibody produced by hybridoma 2C2 contains tryptophan at position 13. In yet another exemplary embodiment, the framework between CDR1 and CDR2 of a variable light chain region of the antibody produced by hybridoma 2C2 contains proline at position 12 and tryptophan at position 13. The position of the amino acid of SEQ ID NO: 12 is illustrated below:















Amino Acid























W*
Y
Q
Q
K
P
G
S
S
P
K
P
W
I
Y**


























Position
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15


No.





*The amino acid at position 1 of FW2 (W) is the residue adjacent to CDR 1.


**The amino acid at position 15 of FW2 (Y) is the residue adjacent to CDR2.






In one embodiment, the antibodies or antigen-binding portions thereof further comprises a framework at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to SEQ ID NO: 85 (Heavy Chain Framework 3) or SEQ ID NO: 89 (Heavy Chain Framework 3 of Humanized Antibody, see in Table 1), wherein the framework is between CDR2 and CDR3 of a variable heavy chain region of the antibody produced by hybridoma 2C2. In another embodiment, the antibodies or antigen-binding portions thereof further comprises a framework at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to SEQ ID NO: 86 (Light Chain Framework 3) or SEQ ID NO: 90 (Light Chain Framework 3 of Humanized Antibody, see Table 1) and said framework is between CDR2 and CDR3 of a variable light chain region of the antibody produced by hybridoma 2C2.


A variable heavy chain region of the antibodies or antigen-binding portions thereof can comprise one, two three or more CDRs comprise amino acid sequences that are at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to CDRs of the variable heavy chain region of the antibody produced by hybridoma 7A11 (SEQ ID NOs: 23, 24 and 25), and a variable light chain region of the antibodies or antigen-binding portions thereof can comprise one, two three or more CDRs comprise amino acid sequences that are at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to CDRs of a variable light chain region of the antibody produced by hybridoma 7A11 (SEQ ID NOs: 26, 27 and 28).


A variable heavy chain region of the antibodies or antigen-binding portions thereof can comprise one, two three or more CDRs comprise amino acid sequences that are at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to CDRs of the variable heavy chain region of the antibody produced by hybridoma 2F8 (SEQ ID NOs: 31, 32 and 33) or CDRs of the variable heavy chain region of the antibody produced by hybridoma 1E1 (SEQ ID NOs: 39, 40 and 41), and a variable light chain region of the antibodies or antigen-binding portions thereof can comprise one, two three or more CDRs comprise amino acid sequences that are at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, about 70%, about 75%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% homologous to CDRs of the variable light chain region of the antibody produced by hybridoma 2F8 (SEQ ID NOs: 34, 35 and 36) or CDRs of the variable light chain region of the antibody produced by hybridoma 1E1 (SEQ ID NOs: 42, 43 and 44).


In certain embodiments, the variable regions corresponding to the variable regions in Table 1 have sequence variations. For example, a heavy chain variable region, in which 1, 2, 3, 4, 5, 6, 7 or 8 residues, or less than 40%, less than about 30%, about 25%, about 20%, about 15%, about 10%, about 9%, about 8%, about 7%, about 6%, about 5%, about 4%, about 3%, about 2% or about 1% amino acid residues substituted or deleted but retain essentially the same immunological properties including, but not limited to, binding to a carbohydrate antigen.


In certain embodiments, CDRs corresponding to the CDRs in Table 1 have sequence variations. For example, CDRs, in which 1, 2, 3, 4, 5, 6, 7 or 8 residues, or less than 20%, less than 30%, or less than about 40% of total residues in the CDR, are substituted or deleted can be present in an antibody (or antigen-binding portion thereof) that binds a carbohydrate antigen.


The antibodies or antigen-binding portions may be peptides. Such peptides can include variants, analogs, orthologs, homologs and derivatives of peptides, that exhibit a biological activity, e.g., binding of a carbohydrate antigen. The peptides may contain one or more analogs of an amino acid (including, for example, non-naturally occurring amino acids, amino acids which only occur naturally in an unrelated biological system, modified amino acids from mammalian systems etc.), peptides with substituted linkages, as well as other modifications known in the art.


Also within the scope of the invention are antibodies or antigen-binding portions thereof in which specific amino acids have been substituted, deleted or added. In an exemplary embodiment, these alternations do not have a substantial effect on the peptide's biological properties such as binding affinity. In another exemplary embodiment, antibodies may have amino acid substitutions in the framework region, such as to improve binding affinity of the antibody to the antigen. In yet another exemplary embodiment, a selected, small number of acceptor framework residues can be replaced by the corresponding donor amino acids. The donor framework can be a mature or germline human antibody framework sequence or a consensus sequence. Guidance concerning how to make phenotypically silent amino acid substitutions is provided in Bowie et al., Science, 247: 1306-1310 (1990). Cunningham et al., Science, 244: 1081-1085 (1989). Ausubel (ed.), Current Protocols in Molecular Biology, John Wiley and Sons, Inc. (1994). T. Maniatis, E. F. Fritsch and J. Sambrook, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor laboratory, Cold Spring Harbor, N.Y. (1989). Pearson, Methods Mol. Biol. 243:307-31 (1994). Gonnet et al., Science 256:1443-45 (1992).


The antibody, or antigen-binding portion thereof, can be derivatized or linked to another functional molecule. For example, an antibody can be functionally linked (by chemical coupling, genetic fusion, noncovalent interaction, etc.) to one or more other molecular entities, such as another antibody, a detectable agent, a cytotoxic agent, a pharmaceutical agent, a protein or peptide that can mediate association with another molecule (such as a streptavidin core region or a polyhistidine tag), amino acid linkers, signal sequences, immunogenic carriers, or ligands useful in protein purification, such as glutathione-S-transferase, histidine tag, and staphylococcal protein A. One type of derivatized protein is produced by crosslinking two or more proteins (of the same type or of different types). Suitable crosslinkers include those that are heterobifunctional, having two distinct reactive groups separated by an appropriate spacer (e.g., m-maleimidobenzoyl-N-hydroxysuccinimide ester) or homobifunctional (e.g., disuccinimidyl suberate). Such linkers are available from Pierce Chemical Company, Rockford, Ill. Useful detectable agents with which a protein can be derivatized (or labeled) include fluorescent compounds, various enzymes, prosthetic groups, luminescent materials, bioluminescent materials, and radioactive materials. Non-limiting, exemplary fluorescent detectable agents include fluorescein, fluorescein isothiocyanate, rhodamine, and, phycoerythrin. A protein or antibody can also be derivatized with detectable enzymes, such as alkaline phosphatase, horseradish peroxidase, beta-galactosidase, acetylcholinesterase, glucose oxidase and the like. A protein can also be derivatized with a prosthetic group (e.g., streptavidin/biotin and avidin/biotin).


Nucleic acids encoding a functionally active variant of the present antibody or antigen-binding portion thereof are also encompassed by the present invention. These nucleic acid molecules may hybridize with a nucleic acid encoding any of the present antibody or antigen-binding portion thereof under medium stringency, high stringency, or very high stringency conditions. Guidance for performing hybridization reactions can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. 6.3.1-6.3.6, 1989, which is incorporated herein by reference. Specific hybridization conditions referred to herein are as follows: 1) medium stringency hybridization conditions: 6×SSC at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 60° C.; 2) high stringency hybridization conditions: 6×SSC at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 65° C.; and 3) very high stringency hybridization conditions: 0.5 M sodium phosphate, 7% SDS at 65° C., followed by one or more washes at 0.2×SSC, 1% SDS at 65° C.


A nucleic acid encoding the present antibody or antigen-binding portion thereof may be introduced into an expression vector that can be expressed in a suitable expression system, followed by isolation or purification of the expressed antibody or antigen-binding portion thereof. Optionally, a nucleic acid encoding the present antibody or antigen-binding portion thereof can be translated in a cell-free translation system. U.S. Pat. No. 4,816,567. Queen et al., Proc Natl Acad Sci USA, 86:10029-10033 (1989).


The present antibodies or antigen-binding portions thereof can be produced by host cells transformed with DNA encoding light and heavy chains (or portions thereof) of a desired antibody. Antibodies can be isolated and purified from these culture supernatants and/or cells using standard techniques. For example, a host cell may be transformed with DNA encoding the light chain, the heavy chain, or both, of an antibody. Recombinant DNA technology may also be used to remove some or all of the DNA encoding either or both of the light and heavy chains that is not necessary for binding, e.g., the constant region.


The present nucleic, acids can be expressed in various suitable cells, including prokaryotic and eukaryotic cells, e.g., bacterial cells, (e.g., E. coli), yeast cells, plant cells, insect cells, and mammalian cells. A number of mammalian cell lines are known in the art and include immortalized cell lines available from the American Type Culture Collection (ATCC). Non-limiting examples of the cells include all cell lines of mammalian origin or mammalian-like characteristics, including but not limited to, parental cells, derivatives and/or engineered variants of monkey kidney cells (COS, e.g., COS-1, COS-7), HEK293, baby hamster kidney (BHK, e.g., BHK21), Chinese hamster ovary (CHO), NS0, PerC6, BSC-1, human hepatocellular carcinoma cells (e.g., Hep G2), SP2/0, HeLa, Madin-Darby bovine kidney (MDBK), myeloma and lymphoma cells. The engineered variants include, e.g., glycan profile modified and/or site-specific integration site derivatives.


The present invention also provides for cells comprising the nucleic acids described herein. The cells may be a hybridoma or transfectant, such as hybridoma designated as 2C2.


Alternatively, the present antibody or antigen-binding portion thereof can be synthesized by solid phase procedures well known in the art. Solid Phase Peptide Synthesis: A Practical Approach by E. Atherton and R. C. Sheppard, published by IRL at Oxford University Press (1989). Methods in Molecular Biology, Vol. 35: Peptide Synthesis Protocols (ed. M. W. Pennington and B. M. Dunn), chapter 7. Solid Phase Peptide Synthesis, 2nd Ed., Pierce Chemical Co., Rockford, Ill. (1984). G. Barany and R. B. Merrifield, The Peptides: Analysis, Synthesis, Biology, editors E. Gross and J. Meienhofer, Vol. 1 and Vol. 2, Academic Press, New York, (1980), pp. 3-254. M. Bodansky, Principles of Peptide Synthesis, Springer-Verlag, Berlin (1984).


The present invention provides for methods for making an antibody or antigen-binding portion thereof that specifically binds to a carbohydrate antigen, (e.g., Globo H). For example, a non-human animal is immunized with a composition that includes a carbohydrate antigen (e.g., Globo H), and then a specific antibody is isolated from the animal. The method can further include evaluating binding of the antibody to a carbohydrate antigen.


Any of a variety of carbohydrate antigens, particularly Globo H, may be used in the practice of the present invention. Examples of carbohydrate antigens include, but are not limited to Globo antigens such as Globo H, stage-specific embryonic antigen 3 (SSEA-3) (also called Gb5), stage-specific embryonic antigen 4 (SSEA-4), Gb-4 and Gb-3, Lewis antigens such as sLex, Lex, sLea, Lea and Ley, polysaccharide antigens such as polysialic acid (PSA), sTn(c) amd Tn(c), Thomsen-Friedenreich antigen (TF(c)), the ganglioside such as GD1, GD2, GD3, Fucosyl GM1, GM1, GM2, GM3, GD1α and GM2, sulfitide antigen such as 6Gal-HSO3-SiaLex and 6GluNAc-HSO3-SiaLex. Other carbohydrate antigens include, but are not limited to: α-Galactose, α-Man-6-phosphate, α-L-Rhamnose, α-GalNAc(Tn), α-NeuAc-OCH2C6H4-p-NHCOOCH2, Fucα1-2Galβ1-4GalNAcβ (H types3), NeuAcα2-8NeuAcα, (NeuAcα2-8)2 Polysialic acid, NeuAca2-6Galb, NeuAcb2-6Gala(STn), Gala1-3Galb1-4GlaNAcb (NeuAca2-8)3, GalNAcαa-3(Fucα1-2)Galβ (Blood Group A), Galα1-3(Fucα1-2)Galβ (Blood Group B), 6Gal-HSO3-SiaLex, 6GluNAc-HSO3-SiaLex and α2-6 sialylated diantennary N-glycans.


In one embodiment, the anti-Globo H antibody or the antigen binding portion thereof can cross react or bind with other carbohydrate antigens with high selectivity, as illustrated in FIG. 3A and FIG. 3B. Non limiting examples of the carbohydrate antigens are: SSEA-3, SSEA-4, Lewis antigens.


In one embodiment, the present invention provides for a method for making a hybridoma that expresses an antibody that specifically binds to a carbohydrate antigen (e.g., Globo H). The method contains the following steps: immunizing an animal with a composition that includes a carbohydrate antigen (e.g., Globo H); isolating splenocytes from the animal; generating hybridomas from the splenocytes; and selecting a hybridoma that produces an antibody that specifically binds to Globo H. Kohler and Milstein, Nature, 256: 495, 1975. Harlow, E. and Lane, D. Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1988.


In one embodiment, carbohydrate antigen is used to immunize mice subcutaneously. One or more boosts may or may not be given. The titers of the antibodies in the plasma can be monitored by, e.g., ELISA (enzyme-linked immunosorbant assay) or flow cytometry. Mice with sufficient titers of anti-carbohydrate antigen antibodies are used for fusions. Mice may or may not be boosted with antigen 3 days before sacrifice and removal of the spleen. The mouse splenocytes are isolated and fused with PEG to a mouse myeloma cell line. The resulting hybridomas are then screened for the production of antigen-specific antibodies. Cells are plated, and then incubated in selective medium. Supernatants from individual wells are then screened by ELISA for human anti-carbohydrate antigen monoclonal antibodies. The antibody secreting hybridomas are replated, screened again, and if still positive for anti-carbohydrate antigen antibodies, can be subcloned by limiting dilution.


Adjuvants that may be used to increase the immunogenicity of one or more of the carbohydrate antigens. Non-limiting examples of adjuvants include aluminum phosphate, aluminum hydroxide, MF59 (4.3% w/v squalene, 0.5% w/v polysorbate 80 (Tween 80), 0.5% w/v sorbitan trioleate (Span 85)), CpG-containing nucleic acid, QS21 (saponin adjuvant), α-Galactosyl-ceramides or synthetic analogs thereof (e.g., C34, see U.S. Pat. No. 8,268,969), MPL (Monophosphoryl Lipid A), 3DMPL (3-O-deacylated MPL), extracts from Aquilla, ISCOMS (see, e.g., Sjolander et al. (1998) J. Leukocyte Biol. 64:713; WO90/03184; WO96/11711; WO 00/48630; WO98/36772; WO00/41720; WO06/134423 and WO07/026190), LT/CT mutants, poly(D,L-lactide-co-glycolide) (PLG) microparticles, Quil A, interleukins, Freund's, N-acetyl-muramyl-L-threonyl-D-isoglutamine (thr-MDP), N-acetyl-nor-muramyl-L-alanyl-D-isoglutamine (CGP 11637, referred to as nor-MDP), N-acetylmuramyl-L-alanyl-D-isoglutaminyl-L-alanine-2-(1′-2′-dip-almitoyl-sn-glycero-3-hydroxyphosphoryloxy)-ethylamine (CGP 19835A, referred to as MTP-PE), and RIBI, which contains three components extracted from bacteria, monophosphoryl lipid A, trehalose dimycolate and cell wall skeleton (MPL+TDM+CWS) in a 2% squalene/Tween 80 emulsion.


The immunized animal can be any animal that is capable of producing recoverable antibodies when administered an immunogen, such as, but not limited to, rabbits, mice, rats, hamsters, goats, horses, monkeys, baboons and humans. In one aspect, the host is transgenic and produces human antibodies, e.g., a mouse expressing the human immunoglobulin gene segments. U.S. Pat. Nos. 8,236,311; 7,625,559 and 5,770,429, the disclosure of each of which is incorporated herein by reference in its entirety. Lonberg et al., Nature 368(6474): 856-859, 1994. Lonberg, N., Handbook of Experimental Pharmacology 113:49-101, 1994. Lonberg, N. and Huszar, D., Intern. Rev. Immunol., 13: 65-93, 1995. Harding, F. and Lonberg, N., Ann. N.Y. Acad. Sci., 764:536-546, 1995.


After the host is immunized and the antibodies are produced, the antibodies are assayed to confirm that they are specific for the antigen of interest and to determine whether they exhibit any cross reactivity with other antigens. One method of conducting such assays is a sera screen assay as described in U.S. Patent Publication No. 2004/0126829. Anti-carbohydrate antigen antibodies can be characterized for binding to the carbohydrate by a variety of known techniques. For example, in an ELISA, microtiter plates are coated with the toxin or toxoid antigen in PBS, and then blocked with irrelevant proteins such as bovine serum albumin (BSA) diluted in PBS. Dilutions of plasma from toxin-immunized mice are added to each well and incubated. The plates are washed and then incubated with a secondary antibody conjugated to an enzyme (e.g., alkaline phosphatase). After washing, the plates are developed with the enzyme's substrate (e.g., ABTS), and analyzed at a specific OD. In other embodiments, to determine if the selected monoclonal antibodies bind to the target carbohydrate antigen or epitopes, the antibody can be biotinylated which can then be detected with a streptavidin labeled probe. Anti-carbohydrate antigen antibodies can be tested for reactivity with the carbohydrate by Western blotting.


Hybridomas that produce antibodies that bind, preferably with high affinity, to the carbohydrate antigen, can than be subcloned and further characterized. One clone from each hybridoma, which retains the reactivity of the parent cells (by ELISA), can then be chosen for making a cell bank, and for antibody purification.


Antibodies, or antigen-binding fragments, variants or derivatives thereof of the present disclosure can also be described or specified in terms of their binding affinity to an antigen. The affinity of an antibody for a carbohydrate antigen can be determined experimentally using any suitable method (see, e.g., Berzofsky et al., “Antibody-Antigen Interactions,” In Fundamental Immunology, Paul, W. E., Ed., Raven Press: New York, N.Y. (1984); Kuby, Janis Immunology, W. H. Freeman and Company: New York, N.Y. (1992); and methods described herein). The measured affinity of a particular antibody-carbohydrate antigen interaction can vary if measured under different conditions (e.g., salt concentration, pH). Thus, measurements of affinity and other antigen-binding parameters (e.g., KD, Ka, Kd) are preferably made with standardized solutions of antibody and antigen, and a standardized buffer.


The present antibodies or antigen-binding portions thereof have in vitro and in vivo therapeutic, prophylactic, and/or diagnostic utilities. For example, these antibodies can be administered to cells in culture, e.g., in vitro or ex vivo, or to a subject, e.g., in vivo, to treat, inhibit, prevent relapse, and/or diagnose cancer.


The antibodies or antigen-binding portions thereof can be used on cells in culture, e.g., in vitro or ex vivo. For example, cells can be cultured in vitro in culture medium and contacted by the anti-Globo H antibody or fragment thereof. The methods can be performed on cells present in a subject, as part of an in vivo (e.g., therapeutic or prophylactic) protocol. For in vivo embodiments, the contacting step is effected in a subject and includes administering an anti-toxin antibody or portion thereof to the subject under conditions effective to permit binding of the antibody, or portion thereof, to a carbohydrate antigen (e.g., Globo H) expressed on one or more cancer cells in the subject, e.g., in the breast cancer cell.


The antibody or antigen-binding portion thereof can be administered alone or in combination with another therapeutic agent, e.g., a second monoclonal or polyclonal antibody or antigen-binding portion thereof or a chemotherapeutic agent. The combination product may be a mixture of the two compounds or they may be covalently attached. In one example, the antibody or antigen-binding portion thereof specifically binds to Globo H is combined with an antibody (monoclonal or polyclonal) or antigen-binding portion thereof specifically binds VEGF. In another example, the second agent is a chemotherapy agent (e.g., cyclophosphamide, 5-fluorouracil or Actinomycin-D). The antibodies can also be administered in combinations with a cancer vaccine, e.g., Globo H conjugated with Diphtheria Toxin and a saponin adjuvant.


Methods for Inhibiting Cancer Cells


The invention also provides methods for inhibiting the growth of a cell in vitro, ex vivo or in vivo, wherein the cell, such as a cancer cell, is contacted with an effective amount of an antibody or an antigen-binding portion thereof as described herein. Pathological cells or tissue such as hyperproliferative cells or tissue may be treated by contacting the cells or tissue with an effective amount of an antibody or an antigen-binding portion thereof of this invention. The cells, such as cancer cells, can be primary cancer cells or can be cultured cells available from tissue banks such as the American Type Culture Collection (ATCC). The pathological cells can be cells of a Glolob H expressing cancer, gliomas, meningiomas, pituitary adenomas, or a CNS metastasis from a systemic cancer, lung cancer, prostate cancer, breast cancer, hematopoietic cancer or ovarian cancer. The cells can be from a vertebrate, preferably a mammal, more preferably a human. U.S. Patent Publication No. 2004/0087651. Balassiano et al. (2002) Intern. J. Mol. Med. 10:785-788. Thorne, et al. (2004) Neuroscience 127:481-496. Fernandes, et al. (2005) Oncology Reports 13:943-947. Da Fonseca, et al. (2008) Surgical Neurology 70:259267. Da Fonseca, et al. (2008) Arch. Immunol. Ther. Exp. 56:267-276. Hashizume, et al. (2008) Neuroncology 10:112-120. In one embodiment, the cancer is Globo H expressing cancer. In another embodiment, the cancer is SSEA-3 expressing cancer. In yet another embodiment, the cancer is SSEA-4 expressing cancer. Globo H expressing cancer, SSEA-3 expressing cancer and SSEA-4 expressing cancer include, but are not limited to, breast cancer, lung cancer, prostate cancer, pancreatic cancer, gastric cancer, ovarian cancer and endometrial cancer and colon cancer, liver cancer, nasopharyngeal cancer, skin cancer, oral cancer, renal cancer, brain cancer, cervical cancer and bladder cancer.


In vitro efficacy of the present antibody or the antigen-binding portion thereof can be determined using methods well known in the art. For example, the cytoxicity of the antibody or the antigen-binding portion thereof may be studied by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] cytotoxicity assay. MTT assay is based on the principle of uptake of MTT, a tetrazolium salt, by metabolically active cells where it is metabolized into a blue colored formazon product, which can be read spectrometrically. J. of Immunological Methods 65: 55 63, 1983. The cytoxicity of the present antibody or the antigen-binding portion thereof may be studied by colony formation assay. Functional assays for binding Globo H antigen may be performed via ELISA. Cell cycle block by the antibody or the antigen-binding thereof may be studied by standard propidium iodide (PI) staining and flow cytometry. Invasion inhibition may be studied by Boyden chambers. In this assay a layer of reconstituted basement membrane, Matrigel, is coated onto chemotaxis filters and acts as a barrier to the migration of cells in the Boyden chambers. Only cells with invasive capacity can cross the Matrigel barrier. Other assays include, but are not limited to cell viability assays, apoptosis assays, and morphological assays.


Assays can also be done in vivo using a murine model. See, e.g., B. Teicher, Tumor Models for Efficacy Determination. Mol Cancer Ther 2006; 5: 2435-2443.”


Pharmaceutical Composition


In one embodiment, the present invention provides pharmaceutical compositions comprising an antibody or antigen-binding portion thereof described herein, and a pharmaceutically acceptable carrier. In another embodiment, the pharmaceutical composition comprises an isolated nucleic acid encoding the present antibody or antigen-binding portion thereof, and a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers include any and all solvents, dispersion media, isotonic and absorption delaying agents, and the like that are physiologically compatible. In one embodiment, the composition is effective to inhibit cancer cells in a subject.


Routes of administration of the present pharmaceutical compositions include, but are not limited to, intravenous, intramuscular, intranasal, subcutaneous, oral, topical, subcutaneous, intradermal, transdermal, subdermal, parenteral, rectal, spinal, or epidermal administration.


The pharmaceutical compositions of the present invention can be prepared as injectables, either as liquid solutions or suspensions, or as solid forms which are suitable for solution or suspension in liquid vehicles prior to injection. The pharmaceutical composition can also be prepared in solid form, emulsified or the active ingredient encapsulated in liposome vehicles or other particulate carriers used for sustained delivery. For example, the pharmaceutical composition can be in the form of an oil emulsion, water-in-oil emulsion, water-in-oil-in-water emulsion, site-specific emulsion, long-residence emulsion, stickyemulsion, microemulsion, nanoemulsion, liposome, microparticle, microsphere, nanosphere, nanoparticle and various natural or synthetic polymers, such as nonresorbable impermeable polymers such as ethylenevinyl acetate copolymers and Hytrel® copolymers, swellable polymers such as hydrogels, or resorbable polymers such as collagen and certain polyacids or polyesters such as those used to make resorbable sutures, that allow for sustained release of the pharmaceutical composition.


The present antibodies or antigen-binding portions thereof are formulated into pharmaceutical compositions for delivery to a mammalian subject. The pharmaceutical composition is administered alone, and/or mixed with a pharmaceutically acceptable vehicle, excipient or carrier. Suitable vehicles are, for example, water, saline, dextrose, glycerol, ethanol, or the like, and combinations thereof. In addition, the vehicle can contain minor amounts of auxiliary substances such as wetting or emulsifying agents, pH buffering agents, or adjuvants. Pharmaceutically acceptable carriers can contain a physiologically acceptable compound that acts to, e.g., stabilize, or increase or decrease the absorption or clearance rates of the pharmaceutical pharmaceutical compositions of the invention. Physiologically acceptable compounds can include, e.g., carbohydrates, such as glucose, sucrose, or dextrans, antioxidants, such as ascorbic acid or glutathione, chelating agents, low molecular weight proteins, detergents, liposomal carriers, or excipients or other stabilizers and/or buffers. Other physiologically acceptable compounds include wetting agents, emulsifying agents, dispersing agents or preservatives. See e.g., the 21st edition of Remington's Pharmaceutical Science, Mack Publishing Company, Easton, Pa. (“Remington's”). The pharmaceutical compositions of the present invention can also include ancillary substances, such as pharmacological agents, cytokines, or other biological response modifiers.


Furthermore, the pharmaceutical compositions can be formulated into pharmaceutical compositions in either neutral or salt forms. Pharmaceutically acceptable salts include the acid addition salts (formed with the free amino groups of the active polypeptides) and which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or organic acids such as acetic, oxalic, tartaric, mandelic, and the like. Salts formed from free carboxyl groups can also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, 2-ethylamino ethanol, histidine, procaine, and the like.


Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in the art. See, e.g., Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, Pa., 21st edition.


Pharmaceutical compositions can be administered in a single dose treatment or in multiple dose treatments on a schedule and over a time period appropriate to the age, weight and condition of the subject, the particular composition used, and the route of administration, whether the pharmaceutical composition is used for prophylactic or curative purposes, etc. For example, in one embodiment, the pharmaceutical composition according to the invention is administered once per month, twice per month, three times per month, every other week (qow), once per week (qw), twice per week (biw), three times per week (tiw), four times per week, five times per week, six times per week, every other day (qod), daily (qd), twice a day (qid), or three times a day (tid).


The duration of administration of an antibody according to the invention, e.g., the period of time over which the pharmaceutical composition is administered, can vary, depending on any of a variety of factors, e.g., subject response, etc. For example, the pharmaceutical composition can be administered over a period of time ranging from about one or more seconds to one or more hours, one day to about one week, from about two weeks to about four weeks, from about one month to about two months, from about two months to about four months, from about four months to about six months, from about six months to about eight months, from about eight months to about 1 year, from about 1 year to about 2 years, or from about 2 years to about 4 years, or more.


For ease of administration and uniformity of dosage, oral or parenteral pharmaceutical compositions in dosage unit form may be used. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.


The data obtained from the cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. In one embodiment, the dosage of such compounds lies within a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage can vary within this range depending upon the dosage form employed and the route of administration utilized. In another embodiment, the therapeutically effective dose can be estimated initially from cell culture assays. A dose can be formulated in animal models to achieve a circulating plasma concentration range that includes the IC50 (i.e., the concentration of the test compound which achieves a half-maximal inhibition of symptoms) as determined in cell culture. Sonderstrup, Springer, Sem. Immunopathol. 25: 35-45, 2003. Nikula et al., Inhal. Toxicol. 4(12): 123-53, 2000.


An exemplary, non-limiting range for a therapeutically or prophylactically effective amount of an antibody or antigen-binding portion of the invention is from about 0.001 to about 60 mg/kg body weight, about 0.01 to about 30 mg/kg body weight, about 0.01 to about 25 mg/kg body weight, about 0.5 to about 25 mg/kg body weight, about 0.1 to about 20 mg/kg body weight, about 10 to about 20 mg/kg body weight, about 0.75 to about 10 mg/kg body weight, about 1 to about 10 mg/kg body weight, about 2 to about 9 mg/kg body weight, about 1 to about 2 mg/kg body weight, about 3 to about 8 mg/kg body weight, about 4 to about 7 mg/kg body weight, about 5 to about 6 mg/kg body weight, about 8 to about 13 mg/kg body weight, about 8.3 to about 12.5 mg/kg body weight, about 4 to about 6 mg/kg body weight, about 4.2 to about 6.3 mg/kg body weight, about 1.6 to about 2.5 mg/kg body weight, about 2 to about 3 mg/kg body weight, or about 10 mg/kg body weight.


The pharmaceutical composition is formulated to contain an effective amount of the present antibody or antigen-binding portion thereof, wherein the amount depends on the animal to be treated and the condition to be treated. In one embodiment, the present antibody or antigen-binding portion thereof is administered at a dose ranging from about 0.01 mg to about 10 g, from about 0.1 mg to about 9 g, from about 1 mg to about 8 g, from about 2 mg to about 7 g, from about 3 mg to about 6 g, from about 10 mg to about 5 g, from about 20 mg to about 1 g, from about 50 mg to about 800 mg, from about 100 mg to about 500 mg, from about 0.01 μg to about 10 g, from about 0.05 μg to about 1.5 mg, from about 10 μg to about 1 mg protein, from about 30 μg to about 500 μg, from about 40 μg to about 300 μg, from about 0.1 □g to about 200 μg, from about 0.1 □g to about 5 □g, from about 5 □g to about 10 □g, from about 10 □g to about 25 □g, from about 25 □g to about 50 □g, from about 50 □g to about 100 □g, from about 100 □g to about 500 □g, from about 500 □g to about 1 mg, from about 1 mg to about 2 mg. The specific dose level for any particular subject depends upon a variety of factors including the activity of the specific peptide, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination and the severity of the particular disease undergoing therapy and can be determined by one of ordinary skill in the art without undue experimentation.


The present antibodies, antigen-binding portions thereof, pharmaceutical compositions and methods can be used in all vertebrates, e.g., mammals and non-mammals, including human, mice, rats, guinea pigs, hamsters, dogs, cats, cows, horses, goats, sheep, pigs, monkeys, apes, gorillas, chimpanzees, rabbits, ducks, geese, chickens, amphibians, reptiles and other animals.


The following examples of specific aspects for carrying out the present invention are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way.


EXAMPLES
Example 1: Hybridoma Fusion and Screening

A classical hybridoma fusion was performed. Mice received their first immunization with Globo H-KLH (Keyhole Limpet Hemocyanin) conjugated with a saponin adjuvant and 3 subsequent boosters on days 7, 14, and 24. Bleedings trials were performed at day 10, 17, 21, and 24 and the serum was tested to check for titres of anti-Globo H antibody. Five mice were found to produce high anti-Globo H IgG and anti-Globo H IgM titers and were used for hybridoma production. Mouse myeloma cells were used for fusion with the mouse splenocytes following procedure of a KÖhler and Milstein (KÖhler G. and Milstein C, 1975). Hybridoma supernatants were screened by affinity ELISA with 0.2 μg Globo H-ceramide/well. The anti-Globo H Vk9 mAb served as positive controls. The OD of hybridoma clone with no dilution of supernatant>background×2 was selected. Top five hybridoma clones were 1E1, 2C2, 2F8, 3D7, 7A11.


Example 2: Kinetic Analysis of Mouse Monoclonal Antibodies

Kinetic binding experiments were performed at 25° C. with Biacore T100 (GE Healthcare) by single-cycle kinetics (SCK) method and multi-cycle kinetics (MCK) method.


Globo H was immobilized by amine coupling, according to the manufacturer's instruction. The Globo H-amine was diluted to 15 mg/ml in immobilization buffer (10 mM sodium acetate pH4.5) and immobilized at 25° C. using a flow rate of 5 μl/min.


The anti-Globo H antibodies (Globo H-Vk9 mab, Globo H-2C2 mAb and Globo H-3D7 mAb) were diluted in running buffer to 200 nM (50 nM). 200 μl of the 200 nM (50 nM) solution was mixed with 200 μl running buffer to obtain a 100 nM (25 nM) solution. The dilution continued for the following dilution series: 200, 100, 50, 25 and 12.5 nM (analyte concentration: 50 nM, 25 nM, 12.5 nM, 6.25 nM, 3.125 nM). The dilution samples were placed in Rack Positions and tested by MCK and SCK methods. Samples for MCK and SCK series were subjected to 420 seconds dissociation time. The surface was regenerated by a 40 seconds injection of a 10 mM Glycine pH2.0/1.5 (v/v=1) solution. The SCK and MCK data were fitted with a 1:1 binding model with Biacore Evaluation software 2.0.


The results were analyzed for the dissociation constant (KD), which is the measure used to describe the binding strength between antibody and antigen, kon(1/Ms), the on-rate at which antibody antigen complexes form, koff(1/s), the off-rate at which the antibody antigen complexes dissociate, Rmax, the maximum amount of analyte response. Table 2 shows affinity and kinetic data for anti-Globo H antibodies from the following hybridomas: VK9, 2C2 and 3D7. 3D7 antibody and 2C2 antibody have a higher binding affinity than that of VK9 antibody









TABLE 2







Kinetic data for Anti-Globo H Antibodies












Hybridoma
KD (M)
kon (l/MS)
koff (1/s)
Rmax
Chi2















VK9
1.436E−7
2.994E+4
4.298E−3
68.3
8.48


3D7
7.854E−9
2.760E+5
2.168E−3
203.5
0.6514


2C2
7.631E−9
2.810E+5
2.144E−3
187.4
0.542









Example 3: Affinity Analysis of Anti-Globo H Antibodies

The following anti-Globo H antibodies were tested for EC50 and % of cell binding: Globo H-VK9 mAb, Globo H-1E1 mAb, Globo H-2C2 mAb, Globo H-2F8 mAb, Globo H-3D7 mAb, Globo H-7A11 mAb.


Procedures:


Affinity ELISA for EC50: The wells were coated with 0.2 μg Globo H-ceramide per well on ice. After blocking, the tested antibody from 6.2 ng/ml to 51200 ng/ml was added to the wells. After incubation for 1 hour at room temperature, the excess antibody was removed by washing for 3 times. Goat anti-mouse IgG-HRP (1:533) was added. Color development was quantified in a plate reader at 490 nm. EC50 was determined by Prism 5.0 Software.


FACS for % of cell binding with Antibody: The cancer cell lines were prepared for a total of 200,000 cells in 50 μl FACS buffer per tube. The indicated antibody was added to reach a final concentration of 1 μg/ml. After gentle vortex, the tubes were placed on ice and incubated for approximately 1 hr. After washing with FACS buffer, the anti-mouse IgG-PE in FACS buffer was added to reach a final concentration of 4 μg/ml. After gentle vortex, the tubes were placed on ice and incubated for approximately 30 mins. After washing with FACS buffer, the test cells were resuspended in 200 μl of FACS buffer. After performing the flow cytometry, the percent of cell binding were analyzed by WinMDI software. In the histogram plot, incubation of secondary antibody only was used to define the background (M1) and binding (M2) region. Based on the setting of secondary antibody only as background (M1), the percentage of binding region (M2) of the indicated antibodywas determined.


Antibody binding to Globo H in breast cancer cell line (MCF-7), lung cancer cell line (LLC1) and pancreatic cell line (HPAC) was evaluated using fluorescence activated cell sorting (FACS) analysis.


Results:


Table 3 summarizes the EC50 data of Globo H-2C2 mAb, Globo H-2F8 mAb, Globo H-3D7 mAb, Globo H-7A11 mAb, and Globo H-1E1 mAb. The results show that Globo H-2C2 mAb, Globo H-2F8 mAb, Globo H-3D7 mAb, and Globo H-7A11 mAb are more effective in neutralizing Globo H antigen compared to Globo H-VK9 mAb. FACS analysis shows that Globo H-2C2 mAb and Globo H-3D7 mAb have a higher binding affinity for Globo H antigen on breast cancer cell line compare to Globo H VK9 mAb. In addition, Globo H-2C2 mAb and Globo H-3D7 mAb have a higher binding affinity for Globo H antigen on pancreatic cancer cell line (HPAC) compare to Globo H VK9 mAb. Moreover, Globo H-1E1 mAb, Globo H-2C2 mAb, Globo H-3D7 mAb and Globo H-7A11 mAb have a higher binding affinity for Globo H antigen on lung cancer cell line (LLC1) compare to Globo H VK9 mAb.









TABLE 3







EC50 and Binding Affinity of Anti-Globo H Antibodies












ELISA






EC50
MCF-7
LLC1
HPAC


Hybridoma
(ng/ml)
% Gated
% Gated
% Gated














VK9
8915.85
82.63
7.67
33.63


1E1
12914.62
70.63
14.61
24.47


2C2
2161.56
87.44
10.24
39.73


2F8
8691.14
65.95
8.87
12.32


3D7
4072.24
86.87
11.00
34.11


7A11
3478.15
59.64
16.70
15.05





The EC50 value is the concentration of antibody which neutralizes 50% of Globo H.






Example 4: In Vivo Anti-Tumor Evaluation of Anti-Globo H Antibodies

Nude mice weighing 33 g with human pancreatic cancer (HPAC) xenograft were randomized into the following 6 study groups:
















Groups in FIG. 1
Treatment
N
Dose
Hybridoma







PBS
PBS (Phosphate
4
0.4 μg/g twice



(Control)
Buffered Saline)

a week via




Alone

intraperitoneal



VK9
Globo H VK9 mAb
4
injection
Vk9


2C2
Globo H 2C2 mAb
4

2C2


3D7
Globo H 3D7 mAb
4

3D7









The mice were observed for a period of 29 days for tumor volume and the results were recorded and summarized in FIG. 1.


Results:


At day 29, the tumor volume was reduced in the following order: VK9=3D7<2C2 groups. The tumor volume in 2C2 group was significantly reduced compare to the control group (P<0.05).


Example 5: In Vivo Anti-Tumor Evaluation of Anti-Globo H Antibodies

Nude mice weighing 27 g with human breast cancer (MCF7) xenograft were randomized into the following 5 study groups:
















Groups in






FIG. 2
Treatment
N
Dose
Hybridoma







Normal
PBS
Alone
8



Saline






(Vehicle)






2C2
Globo H 2C2 mAb
8
0.4 mg/kg on Day 1
2C2


2C2
Globo H 2C2 mAb
8
  4 mg/kg on Day 1
2C2









The mice were observed for a period of 18 days for tumor volume and the results were recorded and summarized in FIG. 2.


Results:


As shown in FIG. 2, the tumor volume was reduced in the following groups compare to control: 2C2 (4 mg/kg)>2C2 (0.4 mg/kg) after Day 15. Since Day 8, the tumor volume in 2C2 (4 mg/kg) was significantly reduced compare to the control group (P<0.05).


Example 6: Cross Reactivity of Anti-Globo H Antibodies

An in vitro cross reactivity evaluation of Anti-Globo H antibodies (Globo H 2C2, 7A11, 3D7, 2F8, 1E1 mAb and Globo H Vk9) was performed.


Procedure:


GlycoDx cartridges were added with 620 μL wash buffer, 100 μL of diluted Anti-Globo H antibodies, 100 μL blocking buffer, 120 μL conjugate buffer, and 120 μL substrate buffer. The cartridge was detected with the CCD analyzer, and the data is output to the Excel spreadsheet for further analysis.


Results:


As shown in FIG. 3A, Globo H 2C2 mAb binds to Globo H and shows cross reactivity to other carbohydrate antigens, such as the Lewis antigens (sLex and sLea) and S15-S27 antigens (see Table 4 for the list of carbohydrate antigens). Globo H 7A11 mAb binds to Globo H and shows cross reactivity to other carbohydrate antigens, such as the Lewis antigens (sLex and sLea) and S15-S17, S19-S22 antigens (see FIG. 3B). Globo H 3D7 mAb binds to Globo H and shows cross reactivity to other carbohydrate antigens, such as the Lewis antigens (sLex, sLea, and Ley) and S15-S22 antigens (see FIG. 3C). Globo H 2F8 mAb binds to Globo H and shows cross reactivity to other carbohydrate antigens, such as the Lewis antigens (sLex and sLea) and S15, S17 and S21 antigens (see FIG. 3D). Globo H 1E1 mAb binds to Globo H and shows cross reactivity to other carbohydrate antigens, such as the Lewis antigens (sLex) and S16, S17 and S20-S22 antigens (see FIG. 3E) In contrast, Globo H VK9 mAb only binds to Globo H and does not show cross-reactivity to other carbohydrate antigens (see FIG. 3F).









TABLE 4







List of carbohydrate antigens in FIG. 3A-3C










Abbreviation
Carbohyrate Antigen






S15
α-GalNAc(Tn)



S16
α-NeuAc-OCH2C6H4-p-




NHCOOCH2



S17
Fucα1-2Galβ1-4GalNAcβ




(H types3)



S18
NeuAcα2-




8NeuAcα,(NeuAcα2-8)2




Polysialic acid



S19
NeuAca2-6Galb



S20
NeuAcb2-6Gala(STn)



S21
Gala1-3Galb1-4GlaNAcb



S22
(NeuAca2-8)3



S25
6Gal-HSO3-SiaLex



S26
6GluNAc-HSO3-




SiaLex



S27
α2-6 sialylated




diantennary N-glycans



S28
GalNAcβ1-




4(NeuAcα2-




8NeuAcα2-3)Galβ1-




4Glc-β-Nac-spacer 3-




Biotin (GD2)



S29
GalNAcβ1-




4(NeuAcα2-3)Galβ1-




4Glc-β-Nac-spacer 3-




Biotin (GM2)



S30
Neu5Acα2-3Galβ1-



(SSEA4)
3GalNAcβ1-3Galα1-




4Galβ1-4Glcβ-Nac-




spacer 3-Biotin




(SSEA4)



S31
NeuAcα2-8NeuAcα2-




3Galβ1-4Glc-β-Nac-




spacer 3-Biotin




(GD3)



S32
Fucα1-2Galβ1-




3GalNAcβ1-




4(Neu5Acα2-3)Galβ1-




4Glcβ-Nac-spacer 3-




Biotin (Fucosyl-GM1)









Example 7: Binding Affinity of Anti-Globo H Antibody

An in vitro evaluation of the following Anti-Globo H humanized antibodies was performed. Table 5 lists the amino acid sequences of the heavy chain regions and light chain regions of the humanized antibody from hybridoma 2C2.









TABLE 5







Amino acid sequences of 2C2 humanized antibody








Light Chain



Region or Heavy



Chain Region
Amino Acid Sequence












Light Chain 2
FW1:
LSPGERATLSC (SEQ ID NO: 88)



CDR1:
RASSSVSYMH (SEQ ID NO: 8)



FW2:
WYQQKPGSSPKPWIY (SEQ ID NO: 12)



CDR2:
ATSNLAS (SEQ ID NO: 9)



FW3:
GVPSRFSGSGSGTDFTFTISSLQPEDIATYYC (SEQ ID NO: 90)



CDR3:
QQWSRNPFT (SEQ ID NO: 10)





Light Chain 2-22
FW1:
SEQ ID NO: 88



CDR1:
SEQ ID NO: 8



FW2:
WYQQKPGSSPKLWIY



CDR2:
SEQ ID NO: 9



FW3:
SEQ ID NO: 90



CDR3:
SEQ ID NO: 10





Light Chain 2-23
FW1:
SEQ ID NO: 88



CDR1:
SEQ ID NO: 8



FW2:
WYQQKPGSSPKPLIY



CDR2:
SEQ ID NO: 9



FW3:
SEQ ID NO: 90



CDR3:
SEQ ID NO: 10





Heavy Chain 2
FW1:
SGPTLVKPTQTLTLTCTFSGFSL (SEQ ID NO: 87)



CDR1:
YTFDMGVG (SEQ ID NO: 5)



FW 2:
WIRQPSGKGLEWLA (SEQ ID NO: 11)



CDR 2:
HIWWDDDKYYNPALKS (SEQ ID NO: 6)



FW3:
RLTISKDTSKNQVVLTMTNMDPVDTATYYCAR (SEQ ID NO: 89)



CDR 3:
VRGLHDYYYWFAY (SEQ ID NO: 7)





Heavy Chain 2-21
FW1:
SEQ ID NO: 87



CDR1:
SEQ ID NO: 5



FW 2:
WIRQPPGKGLEWLA



CDR 2:
SEQ ID NO: 6



FW3:
SEQ ID NO: 89



CDR 3:
SEQ ID NO: 7





Heavy Chain 2-22
FW1:
SEQ ID NO: 87



CDR1:
SEQ ID NO: 5



FW 2:
WIRQPSGKALEWLA



CDR 2:
SEQ ID NO: 6



FW3:
SEQ ID NO: 89



CDR 3:
SEQ ID NO: 7









The results of the binding affinity by ELISA method are listed in Tables 6-9.









TABLE 6







The binding affinity of an antibody with a chimeric light chain


and a humanized heavy chain.











Affinity ELISA


Light Chain
Heavy Chain
(optical density)












Chimeric light chain
Heavy Chain 2-22
0.17


(SEQ ID NO: 4)




Chimeric light chain
Heavy Chain 2
0.4


(SEQ ID NO: 4)











These results show that the alteration of the amino acid at position 9 (from G to A) in FW2 of the heavy chain reduces the binding affinity of the antibody from 0.4 to 0.17.









TABLE 7







The bindinig affinity of an antibody with a humanized


light chain and a humanized heavy chain













Affinity ELISA



Light Chain
Heavy Chain
(optical density)







Light chain 2
Heavy Chain 2
0.42



Light Chain 2-22
Heavy Chain 2
0.08



Light Chain 2-23
Heavy Chain 2
0.09

















TABLE 8







The bindinig affinity of an antibody with a humanized light


chain and a humanized heavy chain













Affinity ELISA



Light Chain
Heavy Chain
(optical density)







Light chain 2
Heavy Chain 2-21
0.53



Light Chain 2-22
Heavy Chain 2-21
0.08



Light Chain 2-23
Heavy Chain 2-21
0.12

















TABLE 9







The bindinig affinity of an antibody with a humanized light


chain and a humanized heavy chain













Affinity ELISA



Light Chain
Heavy Chain
(optical density)







Light chain 2
Heavy Chain 2-22
0.32



Light Chain 2-22
Heavy Chain 2-22
0.07



Light Chain 2-23
Heavy Chain 2-22
0.08










These results show the alteration of the amino acid at position 12 of FW2 of the light chain (from P to L) reduces the binding affinity from 0.32-0.53 to 0.07-0.08, and the alteration of amino acid at position 13 of FW2 of the light chain (from W to L) reduces the binding affinity from 0.32-0.53 to 0.08-0.12.


While specific aspects of the invention have been described and illustrated, such aspects should be considered illustrative of the invention only and not as limiting the invention as construed in accordance with the accompanying claims. All publications and patent applications cited in this specification are herein incorporated by reference in their entirety for all purposes as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference in its entirety for all purposes. Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be readily apparent to one of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications can be made thereto without departing from the spirit or scope of the appended claims.

Claims
  • 1. A method of treating cancer, comprising administering to a patient in need thereof a therapeutically effective amount of an antibody or antigen-binding portion thereof; wherein the antibody or antigen-binding portion thereof comprises: (1) a first heavy chain complementarity determining region (HCDR1) having an amino acid sequence of SEQ ID NO:5;a second heavy chain complementarity determining region (HCDR2) having an amino acid sequence of SEQ ID NO: 6;a third heavy chain complementarity determining region (HCDR3) having an amino acid sequence of SEQ ID NO: 7;a framework between a leader sequence and said HCDR1 of the heavy chain having amino acid sequence at least 85% identical to SEQ ID NO: 87;a framework between said HCDR2 and said HCDR3 of the heavy chain, having an amino acid sequence at least 85% identical to SEQ ID NO: 89;a first light chain complementarity determining region (LCDR1) having an amino acid sequence of SEQ ID NO: 8;a second light chain complementarity determining region (LCDR2) having an amino acid sequence of SEQ ID NO: 9;a third light chain complementarity determining region (LCDR3) having an amino acid sequence of SEQ ID NO: 10;a framework between a leader sequence and said LCDR1 of the light chain having an amino acid sequence at least 85% identical to SEQ ID NO: 88; anda framework between said LCDR2 and said LCDR3 of the light chain, having an amino acid sequence at least 85% identical to SEQ ID NO: 90,wherein the antibody or antigen-binding portion thereof binds to Globo H.
  • 2. The method of claim 1, wherein the cancer is breast cancer, lung cancer, prostate cancer, pancreatic cancer, gastric cancer, ovarian cancer, endometrial cancer, colon cancer, liver cancer, nasopharyngeal cancer, skin cancer, oral cancer, renal cancer, brain cancer, cervical cancer or bladder cancer.
  • 3. A method of treating cancer, comprising administering to a patient in need thereof a therapeutically effective amount of an antibody or antigen-binding portion thereof, wherein the antibody or antigen-binding portion thereof comprises: (1) a heavy chain variable domain comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 3; and a light chain variable domain comprising an amino acid sequence that is a least 85% identical to the amino acid sequence of SEQ II) NO: 4, wherein the heavy chain variable domain comprises SEQ ID NOs: 5-7 and wherein the light chain variable domain comprises SEQ ID NOs: 8-10; or(2) a heavy chain variable domain comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 21; and a light chain variable domain comprising an amino acid sequence that is a least 85% identical to the amino acid sequence of SEQ ID NO: 22; wherein the heavy chain variable domain comprises SEQ ID NOs: 23-2.5 and wherein the light chain variable domain comprises SEQ ID NOs: 26-28; or(3) a heavy chain variable domain comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 29; and a light chain variable domain comprising an amino acid sequence that is a least 85% identical to the amino acid sequence of SEQ ID NO: 30, wherein the heavy chain variable domain comprises SEQ ID NOs: 31-33 and wherein the light chain variable domain comprises SEQ ID NOs: 34-36,wherein the antibody or an antigen-binding portion thereof binds to a carbohydrate antigen and wherein the cancer expresses the carbohydrate antigen.
  • 4. The method of claim 2, wherein the carbohydrate antigen is Globo H, sLex, sTn, Tn, sLea, α-NeuAc-OCH2C6H4-p-NHCOOCH2, Fucαl-2Galβ1-4GalNAcβ3, NeuAcα2-6Galβ, Galα1-3Galβ1-4GlaNAcβ3, (NeuAcα2-8)3, 6Gal-HSO3-SiaLex, 6GluNAc-HSO3-SiaLex, α2-6 sialylated diantennary N-glycan or polysialic acid.
  • 5. The method of claim 3, wherein the antibody or antigen-binding portion thereof is: (a) a whole immunoglobulin molecule; (b) an scFv; (c) a Fab fragment; (d) an F(ab′)2; or (e) a disulfide linked Fv.
  • 6. The method of claim 3, wherein the cancer is a Globo H expressing cancer.
  • 7. The method of claim 3, wherein the cancer is breast cancer, lung cancer, prostate cancer, pancreatic cancer, gastric cancer, ovarian cancer, endometrial cancer, colon cancer, liver cancer, nasopharyngeal cancer, skin cancer, oral cancer, renal cancer, brain cancer, cervical cancer or bladder cancer.
  • 8. A method of treating cancer, comprising administering to a patient in need thereof a therapeutically effective amount of an antibody or antigen-binding portion thereof, wherein the antibody or an antigen-binding portion thereof comprises: a first heavy chain complementarity determining region (HCDR1) having an amino acid sequence of SEQ ID NO: 5;a second heavy chain complementarity determining region (HCDR2) having an amino acid sequence of SEQ ID NO: 6;a third heavy chain complementarity determining region (HCDR3) having an amino acid sequence of SEQ ID NO: 7;a framework between said HCDR1 and said HCDR2 of the heavy chain having amino acid sequence at least 85% identical to SEQ ID NO: 11, wherein the framework contains glycine at position 9;a first light chain complementarity determining region (LCDR1) having an amino sequence of SEQ ID NO: 8;a second light chain complementarity determining region (LCDR2) having an amino acid sequence of SEQ ID NO: 9;a third light chain complementarity determining region (LCDR3) having an amino acid sequence of SEQ ID NO: 10; anda framework between said LCDR1 and said LCDR2 of the light chain having amino acid sequence at least 85% identical to SEQ ID NO: 12, wherein the framework contains proline at position 12 and/or tryptophan at position 13,wherein the antibody or the antigen-binding portion thereof binds to Globo H.
  • 9. The method of claim 8, wherein the cancer is breast cancer, lung cancer, prostate cancer, pancreatic cancer, gastric cancer, ovarian cancer, endometrial cancer, colon cancer, liver cancer, nasopharyngeal cancer, skin cancer, oral cancer, renal cancer, brain cancer, cervical cancer or bladder cancer.
CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No. 15/303,132 filed on Oct. 10, 2016, which is national stage entry of PCT/US2015/025305, filed on Apr. 10, 2015, which claims priority to U.S. Provisional Application Ser. No. 61/977,824, filed on Apr. 10, 2014, and U.S. Provisional Application Ser. No. 62/057,381, filed on Sep. 30, 2014. The entirety of the aforementioned applications is incorporated herein by reference.

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Related Publications (1)
Number Date Country
20180134799 A1 May 2018 US
Provisional Applications (2)
Number Date Country
62057381 Sep 2014 US
61977824 Apr 2014 US
Continuations (1)
Number Date Country
Parent 15303132 US
Child 15824733 US