Claims
- 1. An isolated antibody that binds a receptor protein, said receptor protein to which said antibody is raised comprising a protein in purified form derived from rat liver cell membranes, that recognizes and binds advanced glycosylation endproducts (AGE) and that possesses the following characteristics:
- A. said protein recognizes and binds with the ligands AGE-RNase and AGE-Collagen I;
- B. said protein does not recognize and bind with the ligands FFI-BSA, formaldehyde-treated BSA, glucosamide-BSA, and acetyl LDL-BSA in solid phase ligand blotting assay; and
- C. said protein has a molecular mass of about 90 kD or about 60 kD as determined by protein migration on SDS-PAGE.
- 2. The antibody of claim 1 wherein said protein has the following characteristics:
- A. said protein is found on rat liver cell membranes;
- B. said protein is expressed on rat monocytes and rat macrophages; and
- C. said protein is purified by elutions based, respectively, on an AGE ligand affinity column, and anion exchange column, and a hydroxylapatite column;
- and also bears the following distinction:
- D. when said protein has a molecular mass of about 90 kD, as determined by SDS Page, and it is immobilized on nitrocellulose in a solid phase assay, the 90 kD protein does not bind to AGE-modified ligands, and when the protein has a molecular mass of about 60 kD, as determined by SDS Page, said protein binds to AGE modified ligands when immobilized on nitrocellulose in a solid phase assay.
- 3. The antibody of claim 1 wherein said receptor is isolated and purified by the immobilization of detergent-solubilized rat liver membrane proteins on nitrocellulose in accordance with a solid phase assay protocol.
- 4. The antibody of claim 1 wherein the said protein as determined by SDS Pages has a molecular mass of about 90 kD, and the NH.sub.2 -terminal partial amino acid sequence set forth in FIG. 11 (SEQ ID NO.2).
- 5. The antibody of claim 1 whrein the said protein as determined by SDS Page has a molecular mass of about 60 kD and the NH.sub.2 -terminal partial amino acid sequence set forth in FIG. 12 (SEQ ID NO.1).
- 6. The antibody of claim 1 which is a polyclonal antibody.
- 7. The antibody of claim 1 which is a monoclonal antibody.
- 8. An immortal cell line that produces a monoclonal antibody according to claim 7.
- 9. The antibody of claim 1 labeled with a detectable label.
- 10. The antibody of claim 9 wherein the label is selected from the group consisting of enzymes, chemicals which fluoresce and radioactive elements.
CROSS-REFERENCE TO RELATED APPLICATIONS
This Application is a Division of application Ser. No. 07/749,438 filed Aug. 23, 1991 now U.S. Pat. No. 5,585,344, which is a Continuation-In-Part of application Ser. No. 453,958, filed Dec. 20, 1989 now abandoned, which is in turn, a Division of application Ser. No. 091,534, filed Sep. 3, 1987, now U.S. Pat. No. 4,900,747, issued Feb. 13, 1990, which is in turn, a Continuation-In-Part of application Ser. No. 907,747, filed Sep. 12, 1986, now abandoned; all of the above preceding applications by Helen Vlassara, Michael Brownlee and Anthony Cerami, said Ser. No. 907,747, in turn, a Continuation-In-Part of application Ser. No. 798,032, filed Nov. 14, 1985, by Anthony Cerami, Peter Ulrich and Michael Brownlee, now U.S. Pat. No. 4,758,583, which is, in turn, a Continuation-In-Part of application Ser. No. 590,820, now U.S. Pat. No. 4,665,192, filed Mar. 19, 1984 by Anthony Cerami alone.
Government Interests
This invention was made with partial assistance from grant Nos. AG 8245 and DK 19655 from the National Institutes of Health. The government may have certain rights in this invention.
Non-Patent Literature Citations (7)
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Vlassara, H. et al. Proc. Natl. Acad. Sci. U.S.A. 82:5588 (1985). |
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Radoff, S., et al. Arch Biochem. Biophys. 263:418 (1988). |
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Divisions (2)
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749438 |
Aug 1991 |
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Parent |
91534 |
Sep 1987 |
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Continuation in Parts (4)
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453958 |
Dec 1989 |
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907747 |
Sep 1986 |
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798032 |
Nov 1985 |
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590820 |
Mar 1984 |
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