Claims
- 1. A method of making an antibody comprising:
- a) immunizing an animal with a peptide consisting of SEQ ID NO:1 coupled to an immunogenic carrier;
- b) preparing a hybridoma which produces a monoclonal antibody having the specificity and sensitivity of monoclonal antibodies produced by ATCC Accession No. HB 11111 or ATCC Accession No. HB 11112, and;
- c) isolating the monoclonal antibody produced by the hybridoma.
- 2. The method of claim 1 wherein the animal is immunized with the peptide dissolved or suspended in a physiological buffer.
- 3. The method of claim 1 wherein the animal is immunized with SEQ ID NO:1 coupled to keyhole limpet haemocyanin.
- 4. The method of claim 1 wherein the monoclonal antibody is isolated by binding to Protein G or SEQ ID NO:1.
- 5. A method of making a hybridoma comprising:
- a) immunizing an animal with a peptide consisting essentially of SEQ ID NO:1 coupled to an immunogenic carrier;
- b) obtaining spleen cells from the immunized animal, and;
- c) preparing a hybridoma from the spleen cells which produces a monoclonal antibody having the specificity and sensitivity of monoclonal antibodies produced by ATCC Accession No. HB 11111 or ATCC Accession No. HB 11112.
- 6. The method of claim 5 wherein the animal is immunized with SEQ ID NO:1 coupled to keyhole limpet haemocyanin.
- 7. The method of claim 5 wherein the hybridoma is prepared by fusing the spleen cells of the immunized animal with mouse myeloma cells.
- 8. The method of claim 5 wherein the animal is immunized with the peptide dissolved or suspended in a physiological buffer.
Parent Case Info
This is a division of application Ser. No. 08/250,309, filed May 27, 1994, issued as U.S. Pat. No. 5,478,741 on Dec. 26, 1995 which is a continuation of application Ser. No. 07/945.287, filed Sep. 11, 1992 (abandoned).
Non-Patent Literature Citations (2)
Entry |
Rock et. al. Parasitology (1987) 95: 209-227. |
Palfryman et al. Journal of Immunological Methods, 75 (1984) 383-393. |
Divisions (1)
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Number |
Date |
Country |
Parent |
250309 |
May 1994 |
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Continuations (1)
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Number |
Date |
Country |
Parent |
945287 |
Sep 1992 |
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