Claims
- 1. A process for preparing an antitumor protein hybrid which is expressed by the following formula (II'), which process comprises reacting the substantial fragment Fab of an antitumor immunogloblin which has at least one S-sulfo radical or an active disulfide radical expressed by the following formula (V) with the subunit A of ricin which has at least one thiol radical in the fragment:
- --S--Z (V)
- where Z indicates ##STR4## wherein Fab indicates a moiety which is substantially the fragment Fab of an antitumor immunoglobulin; RA indicates a moiety which is the subunit A of ricin; S.sub.1 and S.sub.2 are both sulfur atoms, S.sub.1 indicating a sulfur atom arising fron the disulfide bond in the immunoglobulin and S.sub.2 a sulfur atom arising from the disulfide bond in ricin; and m represents an integer of 1 to 5.
- 2. A process for preparing an antitumor protein hybrid which is expressed by the following formula (I'), which process comprises binding the substantial fragment Fab of an antitumor immunoglobulin which has at least one thiol radical in the fragment with the subunit A of ricin which has at least one thiol radical in the fragment with the use of a compound which has at least two functional groups capable of reacting with a thiol radical
- Fab--S.sub.1 --X--S.sub.2 --RA).sub.m (I')
- wherein Fab indicates a moiety which is substantially the fragment Fab of an antitumor immunoglobulin; RA indicates a moiety which is the subunit A of ricin; X indicates a divalent organic radical; S.sub.1 and S.sub.2 are both sulfur atoms, S.sub.1 indicating a sulfur atom arising from the disulfide bond in the immunoglobulin and S.sub.2 a sulfur atom arising from the sulfide bond in ricin; and m represents a integer of 1 to 5.
- 3. The process of claim 1, wherein said substantial fragment Fab of an antitumor immunogloblin is the substantial fragment Fab of an antitumor immunogloblin having at least one S-sulfo radical.
Priority Claims (1)
Number |
Date |
Country |
Kind |
54-91634 |
Jul 1979 |
JPX |
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CROSS REFERENCE TO RELATED APPLICATIONS
This application is a divisional application of Ser. No. 170,332, filed July 21, 1980, now U.S. Pat. No. 4,350,626.
US Referenced Citations (4)
Divisions (1)
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Number |
Date |
Country |
Parent |
170332 |
Jul 1980 |
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