Patent Application
20230077821
This application contains a Sequence Listing that has been submitted electronically as an XML file named “00530-0330002_SL_ST26.XML.” The XML file, created on Aug. 10, 2022, is 118,387 bytes in size. The material in the XML file is hereby incorporated by reference in its entirety.
Axons that span long distances enable rapid communication within a neural circuit, but they are particularly vulnerable to injury and degeneration. Therefore, axonal degeneration impairs circuit functionality and is a prominent component of multiple neurologic disorders. Many chemotherapeutic drugs required to treat cancers can injure long-range axons (e.g., peripheral sensory or motor neuron axons), causing chemotherapy-induced peripheral neuropathy (CIPN) with impaired tactile sensation and persistent pain. Currently, the molecular mechanisms of CIPN are not understood, and there are no available treatments. There is an unmet need to provide compositions and methods for treating or preventing CIPN.
The ability to sense, respond to, and perceive music, warning cues, language, and other environmental stimuli depends on the auditory nervous system. The human sense of hearing is made possible by the conversion of sound stimuli into patterns of neural activity that can be integrated with other sensory information to guide behavior, intraspecies communication, and other critical functions. This remarkably rapid processing, which can occur in the space of 10 microseconds, relies on hair cells and their proper innervation by precisely wired spiral ganglion neurons within the cochlea of the inner ear. Successful transmission of the intensity, frequency, and timing of stimuli from hair cells to the brain is contingent on the proper assembly of these auditory circuits during development.
Through precisely organized circuits within the inner ear, sensory information represented by specific displacements of the inner and outer hair cells along the basilar membrane is converted into electrical signals that propagate along the spiral ganglia. Peripheral spiral ganglion axons transmit acoustic information from the hair cells to central projections within the eighth (VIIIth) nerve which innervates the brainstem at the anteroventral cochlear nucleus, the posteroventral cochlear nucleus, and the dorsal cochlear nucleus. Integrating centers within the superior olivary complex and inferior colliculus of the midbrain allow organisms to localize sources in space and process complex features such as the temporal and harmonic properties of speech and music. As spiral ganglion neurons form appropriate connections to provide the critical bridge from the inner ear to the central nervous system, understanding how these neurons initiate their assembly and maintain their processes over time is critical to efforts aimed at producing therapies to treat defects in the auditory nervous system.
This disclosure relates, in part, to methods of treating or preventing chemotherapy-induced peripheral neuropathy or hearing loss using bclw protein, a BH4 domain-containing fragment thereof, or a bclw mimetic. Also disclosed are exemplary bclw mimetics that can be used in the methods described herein.
In a first aspect, the disclosure features a method of treating or preventing chemotherapy-induced peripheral neuropathy (CIPN) in a human subject in need thereof. In one instance, the method involves administering a therapeutically effective amount of a bclw protein or a BH4 domain containing fragment thereof. In another instance, the method involves administering a polypeptide comprising an amino acid sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or 100% identical to the amino acid sequence of the BH4 domain of bclw. In another instance, the method involves administering a polypeptide comprising an amino acid sequence that is identical to the amino acid sequence of the BH4 domain of bclw except for between 1 to 10, 1 to 9, 1 to 8, 1 to 7, 1 to 6, 1 to 5, 1 to 4, 1 to 3, 1 to 2, or 1 amino acid substitution, deletion, or insertion. The amino acid substitution may be conservative or non-conservative. In some cases, the substitutions are on the non-interacting face of the BH4 helix. In some cases, the substitutions are on both the interacting and non-interacting faces of the BH4 helix. The interacting/non-interacting face refers to the face of the helix involved in interacting with IP3R and/or Bax. In yet another instance, the method involves administering a bclw mimetic to the human subject.
In certain embodiments, the bclw protein or the BH4 domain containing fragment thereof comprises or consists of one of SEQ ID NOs.: 2, 3, 46, or 47, or an internally cross-linked variant thereof (e.g., wherein 2 amino acids are replaced by two olefinic side chain containing non-natural amino acids that can form a hydrocarbon staple).
In certain embodiments, the bclw mimetic is an internally cross-linked (e.g., stapled, stitched, or a combination thereof) polypeptide comprising the BH4 domain of bclw. In specific embodiments, the internally cross-linked bclw polypeptide is selected from the group consisting of the amino acid sequence set forth in SEQ ID NOs: 4-32, 50, and 51. In one embodiment, the internally cross-linked bclw polypeptide is ALVADFVGYKLRXKGYXSGA (SEQ ID NO: 50), wherein X is a non-natural amino acid with olefenic side chains. In some embodiments, the internally cross-linked bclw polypeptides has at least 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid substitutions relative to the amino acid sequence set forth in SEQ ID NOs: 4-32, 50, and 51. The amino acid substitutions may be conservative substitutions. In certain embodiments, the human subject has, or is at risk of developing, peripheral neuropathy following treatment with a chemotherapeutic agent. In some embodiments, the chemotherapeutic agent is a microtubule-targeting agent. In certain embodiments, the microtubule-targeting agent is a taxane (e.g., paclitaxel). In certain embodiments, the microtubule-targeting agent is vinblastine, vincristine, vindesine, vinorelbine, vinflunine, docetaxel, discodermolide, eleutherobin, sarcodictyin, epothilone, colchicine, combretastatin, 2-methoxyestradiol, or noscapine. In certain embodiments, the chemotherapeutic agent is an alkylating agent (e.g., oxaliplatin, lomustine, or carmustine). In certain embodiments, the chemotherapeutic agent is an arabinoside (e.g., cytarabine). In certain embodiments, the chemotherapeutic agent is a proteasome inhibitor, a PI3K inhibitor, or a Raf inhibitor. In some embodiments, the bclw mimetic, the BH4 domain containing fragment, and the polypeptide are each 8 to 100 (e.g., 8 to 40, 8 to 30, 8 to 50, 8 to 60) amino acids in length (e.g., 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 32, 35, 40, 45, 50, 60, 70, 75, 80, 90, 100). In some embodiments, the bclw mimetic or the polypeptide is administered to the human subject topically. In certain embodiments, the method further involves administering metformin to the human subject.
In a second aspect, the disclosure provides a method of administering chemotherapy to a human subject in need thereof. The method involves administering an effective amount of a chemotherapeutic agent and a therapeutically effective amount of a bclw protein or a BH4 domain containing fragment thereof, a polypeptide comprising an amino acid sequence that is at least 70% identical to the amino acid sequence of the BH4 domain of bclw, or a bclw mimetic to the human subject.
In one embodiment, the internally cross-linked bclw polypeptide is selected from the group consisting of the amino acid sequence set forth in SEQ ID NOs: 4-32, 50, and 51. In one embodiment, the internally cross-linked bclw polypeptide is ALVADFVGYKLRXKGYXSGA (SEQ ID NO: 50), wherein X is a non-natural amino acid with olefenic side chains. In some embodiments, the internally cross-linked bclw polypeptides has at least 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid substitutions relative to the amino acid sequence set forth in SEQ ID NOs: 4-32, 50, and 51. The amino acid substitutions may be conservative substitutions. In certain cases, the serine in SEQ ID NO:50 or 51 is replaced with a cysteine or a norleucine. In certain embodiments, the polypeptide comprises an amino acid sequence that is at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or 100% identical to the amino acid sequence of the BH4 domain of bclw. In some embodiments, the chemotherapeutic agent is a microtubule-targeting agent, an alkylating agent, an antimetabolite, a folic acid analogue, a spindle poison, a platinum compound, an epipodophyllotoxin, an antibiotic, an EGF-R inhibitor, an Eph-R inhibitor, a p38/JAK kinase inhibitor, a PI3K inhibitor, a MEK inhibitor, a MAPK inhibitor, a Trk inhibitor, a proteasome inhibitor, or a Raf inhibitor. In some embodiments, the microtubule-targeting agent is a taxane (e.g., paclitaxel). In some embodiments, the human subject has a hematological tumor. In certain instances the hematological tumor is selected from the group consisting of acute myeloid leukemia, chronic myeloid leukemia, Hodgkin lymphoma, non-Hodgkin lymphoma, multiple myeloma, acute lymphoblastic leukemia, and chronic lymphocytic leukemia. In some embodiments, the human subject has a solid tumor. In certain instances, the solid tumor is selected from the group consisting of breast cancer, ovarian cancer, and lung cancer. In certain embodiments, the chemotherapeutic agent and the bclw mimetic or the polypeptide are administered sequentially. In some instances, the bclw mimetic or the polypeptide is administered prior to the chemotherapeutic agent. In other instances, the chemotherapeutic agent is administered prior to the bclw mimetic or the polypeptide. In some embodiments, the chemotherapeutic agent and the bclw mimetic or the polypeptide are administered simultaneously. In certain instances, the method further involves administering metformin to the human subject.
In a third aspect, the disclosure relates to method of treating a human subject in need of treatment with chemotherapy. The method involves selecting a human subject that has one or more of: reduced levels of endogenous bclw in the axons of sensory neurons; reduced levels of EphA4 or Eph/5 receptors in sensory neurons; reduced expression of transporters; reduced expression of tubulin; or reduced expression of NGF, BDNF or NT3. The method further involves administering an effective amount of a bclw mimetic or a polypeptide comprising an amino acid sequence that is at least 70% identical to the amino acid sequence of the BH4 domain of bclw to the human subject.
In certain embodiments, the bclw mimetic is an internally cross-linked (e.g., stapled, stitched, or a combination thereof) polypeptide comprising the BH4 domain of bclw. In specific embodiments, the internally cross-linked bclw polypeptide is selected from the group consisting of the amino acid sequence set forth in SEQ ID NOs: 4-32, 50, and 51. In one embodiment, the internally cross-linked bclw polypeptide is ALVADFVGYKLRXKGYXSGA (SEQ ID NO: 50), wherein X is a non-natural amino acid with olefenic side chains. In some embodiments, the internally cross-linked bclw polypeptides has at least 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid substitutions relative to the amino acid sequence set forth in SEQ ID NOs: 4-32, 50, and 51. The amino acid substitutions may be conservative substitutions. In certain embodiments, the polypeptide comprises an amino acid sequence that is at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or 100% identical to the amino acid sequence of the BH4 domain of bclw. In certain embodiments, the chemotherapy comprises a taxane (e.g., paclitaxel).
In another aspect, the disclosure features a method of treating or preventing hearing loss in a human subject in need thereof. The method involves administering to the human subject an effective amount of a bclw protein or a BH4 domain containing fragment thereof, a polypeptide comprising an amino acid sequence that is at least 70% identical to the amino acid sequence of the BH4 domain of bclw, or a bclw mimetic.
In certain embodiments, the bclw protein or the BH4 domain containing fragment thereof comprises or consists of one of SEQ ID NOs.: 2, 3, 46, or 47, or an internally cross-linked variant thereof (e.g., wherein 2 amino acids are replaced by two olefinic side chain containing non-natural amino acids that can form a hydrocarbon staple).
In certain embodiments, the bclw mimetic is an internally cross-linked (e.g., stapled, stitched, or a combination thereof) polypeptide comprising the BH4 domain of bclw. In specific embodiments, the internally cross-linked bclw polypeptide is selected from the group consisting of the amino acid sequence set forth in SEQ ID NOs: 4-32, 50, and 51. In one embodiment, the internally cross-linked bclw polypeptide is ALVADFVGYKLRXKGYXSGA (SEQ ID NO: 50), wherein X is a non-natural amino acid with olefenic side chains. In some embodiments, the internally cross-linked bclw polypeptides has at least 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid substitutions relative to the amino acid sequence set forth in SEQ ID NOs: 4-32, 50, and 51. The amino acid substitutions may be conservative substitutions. In some embodiments, the bclw mimetic, the BH4 domain containing fragment, and the polypeptide are each 8 to 100 (e.g., 8 to 40, 8 to 30, 8 to 50, 8 to 60) amino acids in length (e.g., 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 32, 35, 40, 45, 50, 60, 70, 75, 80, 90, 100). In some embodiments, the bclw mimetic, the BH4 domain containing fragment, or the polypeptide is administered to the human subject by injection into the oval window. In some embodiments, the bclw mimetic, the BH4 domain containing fragment, or the polypeptide is administered to the human subject by topical application to the ear. In some embodiments, the bclw mimetic, the BH4 domain containing fragment, or the polypeptide is administered to a subject by direct delivery via the tympanic membrane. In certain instances, the administration involves the use of a virus (e.g., adenovirus, adeno-associated virus, lentivirus vectors) or using ear drops. In certain embodiments, the human subject has age-related hearing loss. In other embodiments, the human subject has noise-related hearing loss. In yet other embodiments, the human subject has chemotherapy-related hearing loss.
In another aspect the disclosure features a polypeptide comprising or consisting of an internally cross-linked polypeptide selected from the group consisting of SEQ ID NOs.: 4 to 30, 50 and 51. In certain embodiments, in instances where the N-terminal residue of the peptide is not an arginine, an arginine residue is added or substituted.
In yet another aspect, the disclosure provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a polypeptide comprising or consisting of an internally cross-linked polypeptide selected from the group consisting of SEQ ID NOs.: 4 to 32, 50 and 51. In certain embodiments, the pharmaceutical composition is formulated as ear drops.
In another aspect, the disclosure features a virus comprising a polypeptide comprising or consisting of an internally cross-linked polypeptide selected from the group consisting of SEQ ID NOs.: 4 to 30, 50 and 51; or a bclw protein; or a BH4 domain containing fragment thereof; or a polypeptide comprising an amino acid sequence that is at least 70% identical to the amino acid sequence of the BH4 domain of bclw. In certain embodiments, the virus is an adenovirus. In other embodiments, the virus is a an adeno-associate virus. In some embodiments, the virus is a lentivirus. Such viruses are useful in delivering the bclw peptide or protein to the subject.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the exemplary methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present application, including definitions, will control. The materials, methods, and examples are illustrative only and not intended to be limiting.
Other features and advantages of the invention will be apparent from the following detailed description and from the claims.
The present disclosure is based, at least in part, on the finding that a BCL-2 family protein, bclw, a BH4 domain-containing fragment thereof, or bclw mimetics are useful for treating or preventing chemotherapy-induced peripheral neuropathy (CIPN). This disclosure features exemplary bclw mimetics that are internally cross-linked bclw polypeptides or modified bclw polypeptides that contain a sequence based on the BH4 domain of bclw. In addition, this disclosure provides methods for treating or preventing hearing loss (e.g., age-related, noise-induced, chemotherapy-induced). Also provided herein are pharmaceutical compositions and kits that contain at least one of these internally cross-linked bclw polypeptides and/or modified polypeptides, methods of using these internally cross-linked polypeptides and/or modified polypeptides to treat CIPN and hearing loss. Non-limiting aspects and embodiments of these methods are described herein. Any of the aspects described below can be used in any combination in the methods described herein.
Bclw, also known as Bcl-2-like protein 2 is a protein that in humans is encoded by the BCL2L2 gene. Bclw encodes a pro-survival (i.e., anti-apoptotic) member of the bcl-2 protein family and expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. The murine form of this gene has been shown to play a role in the survival of NGF- and BDNF-dependent neurons.
The amino acid sequence of human bclw is provided below (the BH4 domain is boldened):
This disclosure features the use of the bclw protein in the methods described herein. The bclw protein that is used may be at least 65%, 70%, 75%, 80%, 85%, 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to SEQ ID NO: 1. These bclw proteins include the BH4 domain or a functional variant thereof. Methods for determining percent identity between amino acid sequences are known in the art. For example, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). In a preferred embodiment, the length of a reference sequence aligned for comparison purposes is at least 60%, 70%, 80%, 90%, or 100% of the length of the reference sequence. The amino acid residues at corresponding amino acid positions are then compared. When a position in the first sequence is occupied by the same amino acid residue as the corresponding position in the second sequence, then the molecules are identical at that position. The determination of percent identity between two amino acid sequences is accomplished using the BLAST 2.0 program. Sequence comparison is performed using an ungapped alignment and using the default parameters (Blossom 62 matrix, gap existence cost of 11, per residue gapped cost of 1, and a lambda ratio of 0.85). The mathematical algorithm used in BLAST programs is described in Altschul et al. (Nucleic Acids Res., 25:3389-3402, 1997).
In some instances, the bclw protein used in the methods described herein are fragments of bclw that include the BH4 domain. These fragments can be, e.g., 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 125, or 150 amino acids in length. In certain instances, the fragments may be linked to a half-life extending moiety (e.g., polyethylene glycol, human serum albumin, an XTEN).
All proteins belonging to the Bcl-2 family contain either a BH1, BH2, BH3, or BH4 domain. The Bcl-2 family proteins are classified as multidomain anti-apoptotic, multidomain pro-apoptotic, or BH3-only pro-apoptotic proteins. All anti-apoptotic proteins contain BH1 and BH2 domains, and some of them contain an additional N-terminal BH4 domain (e.g., Bcl-2, Bcl-x(L), Bcl-w), which is not seen in pro-apoptotic proteins, except for Bcl-x(S). All pro-apoptotic proteins, except for Bad, and some anti-apoptotic proteins, such as Bcl-2 or Bcl-x(L), contain a BH3 domain that is necessary for dimerization with other proteins of Bcl-2 family and crucial for their killing activity; some of them also contain BH1 and BH2 domains (e.g., Bax, Bak).
The BH4 domain of bclw has the amino acid sequence: DTRALVADFVGYKLRQKGYVCGA (SEQ ID NO:2). In certain instances, the methods employ a bclw polypeptide comprising or consisting of the amino acid sequence: RALVADFVGYKLRQKGYVCGA (SEQ ID NO:3). In some instances, the methods employ a bclw polypeptide comprising or consisting of the amino acid sequence: ALVADFVGYKLRQKGYVCGA (SEQ ID NO:46). In some instances, the methods employ a bclw polypeptide comprising the amino acid sequence: TRALVADFVGYKLRQK (SEQ ID NO:47).
In certain instances, the BH4 domain of bclw has an amino acid sequence that is at least 40%, 45%, 50%, 55%, 60%, 65%. 70%, 75%, 80%, 85%, 90%, 95% identical to the amino acid sequence of SEQ ID NO:2. In certain instances, the BH4 domain of bclw has an amino acid sequence that is at least 40%, 45%, 50%, 55%, 60%, 65%. 70%, 75%, 80%, 85%, 90%, 95% identical to the amino acid sequence of SEQ ID NO:3. In certain instances, the BH4 domain of bclw has an amino acid sequence that is at least 40%, 45%, 50%, 55%, 60%, 65%. 70%, 75%, 80%, 85%, 90%, 95% identical to the amino acid sequence of SEQ ID NO:46. In certain instances, the BH4 domain of bclw has an amino acid sequence that is at least 40%, 45%, 50%, 55%, 60%, 65%. 70%, 75%, 80%, 85%, 90%, 95% identical to the amino acid sequence of SEQ ID NO:47. In some cases the variability from SEQ ID NO:2, 3, 46, or 47 is on the non-interacting alpha-helical face of the BH4 domain (e.g., the domain that interacts with IP3R and/or Bax). In some cases, the variability from SEQ ID NO:2 is on both the interacting and the non-interacting alpha-helical face of the BH4 domain (e.g., the domain that interacts with IP3R and/or Bax).
An alignment of a region comprising the BH4 domains of Bcl-2 (SEQ ID NO:34), Bcl-x(L) (SEQ ID NO:35), Bcl-w (SEQ ID NO:36) are provided below:
In some instances, the methods described herein employ a polypeptide comprising the BH4 domain of bclw or a polypeptide variant thereof. For example, the polypeptide variant may comprise at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen amino acid substitutions relative to the amino acid sequence set forth in SEQ ID NOs:2, 3, 46, or 47. The substitutions can be on the non-interacting alpha-helical face of the BH4 domain (e.g., the domain that interacts with IP3R and/or Bax). In some cases, the substitutions in SEQ ID NO:2 are on both the interacting and the non-interacting alpha-helical face of the BH4 domain (e.g., the domain that interacts with IP3R and/or Bax). In certain instances, a cysteine residue in a BH4 domain of bclw is replaced with a serine. Thus, the disclosure includes amino acid sequences set forth in SEQ ID NOs:2, 3, 46, or 47 except that the cysteine residue in these sequences is replaced with a serine. In certain instances, a cysteine residue in a BH4 domain of bclw is replaced with a norleucine. Thus, the disclosure includes amino acid sequences set forth in SEQ ID NOs:2, 3, 46, or 47 except that the cysteine residue in these sequences is replaced with a norleucine.
In certain instances, the bclw polypeptide that is employed in the methods described herein is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids in length. In certain instances, the bclw polypeptide that is employed in the methods described herein is 5 to 30, 6 to 30, 7 to 30, 8 to 30, 9 to 30, 10 to 30, 11 to 30, 12 to 30, 14 to 30, 15 to 30, 16 to 30, 17 to 30, 18 to 30, 19 to 30, or 20 to 30 amino acids in length.
In some instances, the methods described herein employ a bclw mimetic. For example, the disclosure provides internally cross-linked polypeptides and/or modified polypeptides that contain a sequence based on a BH4 domain of the BCL-2 family protein, bclw, that include at least two modified amino acids joined by an internal (intramolecular) cross-link (or staple), wherein the at least two amino acids are separated by 2 to 6 amino acids (e.g., 2, 3, or 6 amino acids). Stabilized peptides herein include stapled and/or stitched peptides.
Amino acids are the building blocks of the peptides disclosed herein. The term “amino acid” refers to a molecule containing both an amino group and a carboxyl group. Amino acids include alpha-amino acids and beta-amino acids. Amino acids suitable for inclusion in the peptides disclosed herein include, without limitation, natural alpha-amino acids, such as D- and L-isomers of the 20 common naturally-occurring alpha-amino acids found in peptides (e.g., Ala (A), Arg (R), Asn (N), Cys (C), Asp (D), Gln (Q), Glu (E), Gly (G), His (H), Ile (I), Leu (L), Lys (K), Met (M), Phe (F), Pro (P), Ser (S), Thr (T), Trp (W), Tyr (Y), and Val (V)), unnatural (or non-natural) alpha-amino acids (including, but not limited to, α,α-disubstituted and N-alkylated amino acids), natural beta-amino acids (e.g., beta-alanine), and unnatural (or non-natural) beta-amino acids. Amino acids used in the construction of the internally cross-linked polypeptides and modified polypeptides of the present disclosure can be prepared by organic synthesis or obtained by other routes, such as, e.g., degradation of or isolation from a natural source.
There are many known unnatural (or non-natural) amino acids, any of which can be included in the internally cross-linked polypeptides and modified polypeptides of the present invention. Some non-limiting examples of unnatural amino acids are 4-hydroxyproline, desmosine, gamma-aminobutyric acid, beta-cyanoalanine, norvaline, 4-(E)-butenyl-4(R)-methyl-N-methyl-L-threonine, N-methyl-L-leucine, 1-amino-cyclopropanecarboxylic acid, 1-amino-2-phenyl-cyclopropanecarboxylic acid, 1-amino-cyclobutanecarboxylic acid, 4-amino-cyclopentenecarboxylic acid, 3-amino-cyclohexanecarboxylic acid, 4-piperidylacetic acid, 4-amino-1-methylpyrrole-2-carboxylic acid, 2,4-diaminobutyric acid, 2,3-diaminopropionic acid, 2,4-diaminobutyric acid, 2-aminoheptanedioic acid, 4-(aminomethyl)benzoic acid, 4-aminobenzoic acid, ortho-, meta-, and para-substituted phenylalanines (e.g., substituted with —C(═O)C6H5; —CF3; —CN; -halo; —NO2; CH3), disubstituted phenylalanines, substituted tyrosines (e.g., further substituted with -Q=O)C6H5; —CF3; —CN; -halo; —NO2; CH3), and statine. Additionally, amino acids can be derivatized to include amino acid residues that are, e.g., hydroxylated, phosphorylated, sulfonated, acylated, and glycosylated.
A “peptide” or “polypeptide” contains (includes) a polymer of amino acid residues linked together by peptide (amide) bonds. The term(s), as used herein, refer(s) to proteins, polypeptides, and peptides of any size, structure, or function. Typically, a peptide or polypeptide will be at least three amino acids long. A peptide or polypeptide can refer to an individual protein or a collection of proteins. In some instances, peptides can include only natural amino acids, although non-natural amino acids (i.e., compounds that do not occur in nature but that can be incorporated into a polypeptide chain) and/or amino acid analogs known in the art can alternatively be employed. Also, one or more of the amino acids in a peptide or polypeptide can be modified, e.g., by the addition of a chemical entity, e.g., a carbohydrate group, a hydroxyl group, a phosphate group, a farnesyl group, an isofarnesyl group, a fatty acid group, a linker for conjugation, functionalization, and/or other modification. A peptide or polypeptide can be a single molecule or can be a multi-molecular complex, e.g., a protein. A peptide or polypeptide can be a fragment of a naturally-occurring protein or peptide. A peptide or polypeptide can be naturally-occurring, recombinant, or synthetic, or any combination thereof “Dipeptide” refers to two covalently linked amino acids.
Exemplary peptides that can be used to generate the internally cross-linked polypeptides described herein can include a sequence (e.g., at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids, or between 10-30, 15-24, 15-25, 17-25, 15-22, 18-20, 18-22, 18-25, 19-22, 19-25, 20-22, 20-21, 20-22, 20-23, 20-24, 20-25, 20-26, 20-27, 20-28, 20-29, 20-30, 22-35, 22-40, or 22-50 amino acids) that is at least 70% (e.g., at least 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%) identical to the BH4 domain of bclw e.g., that inherently possess or can be induced to have an alpha-helical secondary structure. Additional exemplary peptides that can be used to generate the internally cross-linked polypeptides described herein can contain one or more additional amino acids present in bclw that are N- and/or C-terminal to the conserved BH4 domain. Additional exemplary peptides that can be used to generate the internally cross-linked polypeptides described herein include a one, two, three, or four amino acid N-terminal and/or a one, two, three, or four amino acid C-terminal truncation of the sequence of any one of SEQ ID NOs: 4-32.
In some instances, the internally cross-linked polypeptides e.g. comprise, consist essentially of, or consist of an amino acid sequence selected from the group of RALVADFVGYKLRQKGYVCGA (SEQ ID NO:3) or ALVADFVGYKLRQKGYVCGA (SEQ ID NO:46), RALVADFVGYKLRQKGYV (SEQ ID NO:48), or ALVADFVGYKLRQKGYV (SEQ ID NO:49), wherein between 1, 2, 3, 4, 5, 6 amino acid substitutions (e.g., cysteine to serine, conservative amino acid substitutions) are made in the sequence of SEQ ID NO:3 or SEQ ID NO:46, and wherein: the side chains of two amino acids separated by two to six (inclusive) (e.g., 2, 3, or 6) amino acids are replaced by an internal staple; the side chains of three amino acids are replaced by internal staples and/or an internal stitch; the side chains of four amino acids are replaced by internal staples, internal stiches, or a combination of internal staples and stiches; or the side chains of at least four amino acids are replaced by internal staples, internal stiches, or a combination of internal staples and stiches. In certain cases, two amino acids in SEQ ID NO: 2, 3, or 46-49 are hydrocarbon stapled. In certain instances, three amino acids in SEQ ID NO: 2, 3, or 46-49 are stitched. In some instances, any two amino acids in SEQ ID NO: 2, 3, or 46-49 at positions [i] and [i+3], positions [i] and [i+4], or at positions [i] and [i+7] are replaced by a non-natural amino acid with olefenic side chains. For example, for [i] and [i+3], it could be two S5 pentenyl alanines, or one R-propenyl alanine and one S-pentenyl alanine; for [i] and [i+4] two S-pentenyl alanines may be used; for [i,] and [i+7] one S-pentenyl alanine and one R-octenyl alanine may be used, or one R-pentenyl alanine and one S-octenyl alanine may be used. Such peptides, upon ruthenium catalyzed olefin metathesis can yield “stapled” bclw peptides. In certain instances, the bclw stapled or stitched peptides can also has one, two, three, four, five, six, or seven amino acid substitutions relative to SEQ ID NO: 2, 3, or 46-49. In certain instances, the amino acid substitutions are conservative. For example, a positively charged amino acid can be replaced by R, K, or H; a negatively-charged amino acid can be replaced with D, N, E, or Q; a polar amino acid can be replaced with A, G, V, L, I, W, F, or Y; and serine, threonine or cysteine can be replaced by each other. It is to be understood that non-natural homologues of the amino acid could be substituted in place of the natural amino acid. In certain instances, the bclw stapled or stitched peptides can also has one, two, three, four, or five deletions relative to SEQ ID NO: 2, 3, or 46-49. In certain instances, the bclw stapled or stitched peptides have one, two, three, or four amino acid substitutions relative to SEQ ID NO: 2, 3, or 46-49 and also have one, two, or three deletions relative to SEQ ID NO:2, 3, or 46-49. Bclw peptides with these substitutions and/or deletions can be selected for use in the methods described herein based on their ability to, e.g., reduce or prevent axonal degeneration and/or bind to the endoplasmic reticulum calcium channel, IP3R1. Methods for determining whether a peptide has these properties are, e.g., described in the Examples. In some cases, the internally stapled or stitched bclw polypeptide includes a targeting moiety or a moiety that facilitates cell entry (e.g., 2, 3, 4, 5, 6, 7, 8 or 9 contiguous Arg) at the amino or carboxy terminus.
A “conservative amino acid substitution” is an amino acid substitution that does not reduce (e.g., substantially reduce) binding of the internally cross-linked polypeptide or modified polypeptide to its target protein, e.g., BAX protein or endoplasmic reticulum calcium channel, IP3R1, and can, in some circumstances, improve binding activity. Methods for detecting any reduction in binding can include comparing binding affinity following conservative amino acid substitution, wherein any amino acid substitution that reduces (e.g., substantially reduces) binding are not conservative amino acid substitutions. In some aspects, substantially reduced binding can include binding that is 10% or less, 20% or less, 30% or less, 40% or less, 50% or less, 60% or less, 70% or less, 80% or less, 90% or less, 95% or less, 98% or less, 99% or less, or 100% less than binding of the unsubstituted internally cross-linked polypeptide or modified polypeptide to its target protein, e.g., a BAX protein, or endoplasmic reticulum calcium channel, IP3R1. Methods for assessing interaction between an internally cross-linked polypeptide or modified polypeptide and a target protein, e.g., a BAX protein or endoplasmic reticulum calcium channel, IP3R1, are disclosed herein.
In some instances, a “conservative amino acid substitution” can include substitutions in which one amino acid residue is replaced with another amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., lysine, arginine, and histidine), acidic side chains (e.g., aspartic acid and glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, and cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, and tryptophan), beta-branched side chains (e.g., threonine, valine, and isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, and histidine).
Non-limiting exemplary internally cross-linked bclw peptides that can be used in the methods described herein include any one of:
XALVXDFVGYKLRQKGYV
8ALVADFXGYKLRQKGYV
wherein X is S-pentenyl alanine; wherein “8” is R-octenyl alanine; and wherein “B” is norleucine.
In a different embodiment, each X in each sequence above (i.e., SEQ ID NOs.: 4-32 and 50-53) can be the same non-natural amino acid or different non-natural amino acid. In certain instances, “X” is one of (S)-2-(4-pentenyl)Ala-OH, (R)-2-(4-pentenyl)Ala-OH, (R)-2-(7-octenyl)Ala-OH, (S)-2-(7-octenyl)Ala-OH, 2-amino-2-(pent-4-enyl)hept-6-enoic acid, (R)-2-(2-propenyl)Ala-OH, or (S)-2-(2-propenyl)Ala-OH. In certain embodiments, if a sequence has two “X's” both X's are (S)-2-(4-pentenyl)Ala-OH. In certain embodiments, if a sequence has two “X's” both X's are (R)-2-(4-pentenyl)Ala-OH. In certain embodiments, if a sequence has two “X's” both X's are (R)-2-(7-octenyl)Ala-OH. In certain embodiments, if a sequence has two “X's” both X's are (S)-2-(7-octenyl)Ala-OH. In certain embodiments, if a sequence has two “X's” both X's are 2-amino-2-(pent-4-enyl)hept-6-enoic acid. In certain embodiments, if a sequence has two “X's” both X's are (R)-2-(2-propenyl)Ala-OH. In certain embodiments, if a sequence has two “X's” both X's are (R)-2-(2-propenyl)Ala-OH. In certain embodiments, if a sequence has two “X's” both X's are (S)-2-(2-propenyl)Ala-OH. In certain embodiments, if a sequence has two “X's” both X's are different non-natural amino acids and can, e.g., be selected from the group consisting of S)-2-(4-pentenyl)Ala-OH, (R)-2-(4-pentenyl)Ala-OH, (R)-2-(7-octenyl)Ala-OH, (S)-2-(7-octenyl)Ala-OH, 2-amino-2-(pent-4-enyl)hept-6-enoic acid, (R)-2-(2-propenyl)Ala-OH, and (S)-2-(2-propenyl)Ala-OH. In certain instances, if the two X's are separated by 3 amino acids, the two X's can be different non-natural residues, e.g, the first X is (R)-2-(2-propenyl)Ala-OH or (S)-2-(2-propenyl)Ala-OH and the second X is (S)-2-(2-propenyl)Ala-OH or (R)-2-(2-propenyl)Ala-OH. In certain instances, the “X's” that are in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 can be any residue/substance that permits formation of a staple and/or stitch. In other words, the staple and/or stitch does not need to be a hydrocarbon-staple and/or stitch. Methods of performing different types of stapling are well known in the art (see, e.g., Lactam stapling: Shepherd et al., J. Am. Chem. Soc., 127:2974-2983 (2005); Triazole stapling: Kawamoto et al., J. Med. Chem., 55:1137-1146 (2011); UV-cycloaddition stapling: Madden et al., Bioorg. Med. Chem. Lett., 21:1472-1475 (2011); Disulfide stapling: Jackson et al., Am. Chem. Soc., 113:9391-9392 (1991); Oxime stapling: Haney et al., Chem. Commun., 47:10915-10917 (2011); Thioether stapling: Brunel and Dawson, Chem. Commun., 552-2554 (2005); Photoswitchable stapling: J. R. Kumita et al., Proc. Natl. Acad. Sci. U.S.A., 97:3803-3808 (2000); Double-click stapling: Lau et al., Chem. Sci., 5:1804-1809 (2014); Bis-lactam stapling: J. C. Phelan et al., J. Am. Chem. Soc., 119:455-460 (1997); and Bis-acylation stapling: A. M. Spokoyny et al., J. Am. Chem. Soc., 135:5946-5949 (2013)). In certain instances, the “X's” that are in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 can be any residue that permits formation of a hydrocarbon staple and/or stitch. In certain instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 are lactam stapled. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 are triazole stapled. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 are stapled using a UV-cycloaddition staple. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 are disulfide stapled. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 are oxime stapled. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 are thioether stapled. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 comprise a photoswitchable staple. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 are double-click stapled. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 are bis-lactam stapled. In other instances, the peptides with the sequences recited in SEQ ID NOs.: 4-32 or 50-53, or inserted into SEQ ID NOs.: 2, 3, 46, 48, or 49 comprise a bis-arylation staple. In certain embodiments, the bclw internally cross-linked peptide comprises or consists of the amino acid sequence set forth in SEQ ID NO: 4-33, or a modified version (i.e., a sequence comprising 2 or more (e.g., 3, 4, 5, 6) non-natural olefinic side chain-containing amino acids that can form a hydrocarbon staple) of, an amino sequence set forth in any one of SEQ ID NOs.: 2, 3, 46, 48, or 49.
In some embodiments of any of the methods described herein, the bclw internally cross-linked peptide is 18 to 100 amino acids in length (e.g., between 18 and 90 amino acids in length, between 18 and 80 amino acids in length, between 18 and 70 amino acids in length, between 18 and 60 amino acids in length, between 18 and 50 amino acids in length, between 18 and 40 amino acids in length, between 18 and 30 amino acids in length, between 18 and 25 amino acids in length, between 20 and 40 amino acids in length, between 20 and 35 amino acids in length, between 20 and 30 amino acids in length, between 22 and 40 amino acids in length; between 22 and 35 amino acids in length, between 22 and 30 amino acids in length, between 25 and 40 amino acids in length, between 25 and 35 amino acids in length, or between 25 and 30 amino acids in length).
As disclosed above, internally cross-linked polypeptides or modified polypeptides herein include at least two modified amino acids that together form an internal (intramolecular) cross-link (or staple), wherein the at least two modified amino acids are separated by 2 (i.e., i, i+3), 3 (i.e., i, i+4), or 6 (i.e., i, i+7) amino acids. Additional exemplary relative positions of staple(s) and/or stitch(es) that can be introduced in any of the internally cross-linked polypeptides and modified polypeptides described herein are known in the art. See, e.g., US 2016/0031959 incorporated by reference in its entirety herein.
In the case of a cross-link between i and i+3 the cross-link can be a C7 alkylene or alkenylene. In the case of a cross-link between i and i+4 the cross-link can be a C8 alkylene or alkenylene. In the case of a cross-link between i and i+7 the cross-link can be a C11, C12, or C13 alkylene or alkenylene. When the cross-link is an alkenylene, there can be one or more double bonds.
In the case of a cross-link between i and i+3 the cross-link can be a C6, C7, or C8 alkyl or alkene (e.g., a C6 alkene having a single double bond). In the case of a cross-link between i and i+4 the cross-link can be a C8 alkyl or alkene. In the case of a cross-link between i and i+7 the cross-link can be a C11, C12, or C13 alkyl or alkene (e.g., a C11 alkene having a single double bond). When the cross-link is an alkene, there can be one or more double bonds.
“Peptide stapling” or “hydrocarbon stapling” is a term coined from a synthetic methodology, wherein two olefin-containing side-chains (e.g., cross-linkable side chains) present in a polypeptide chain are covalently joined (e.g., “stapled together”) using a ring-closing metathesis (RCM) reaction to form a cross-linked ring (see, e.g., Blackwell et al., J. Org. Chem., 66: 5291-5302, 2001; Angew et al., Chem. Int. Ed. 37:3281, 1994). As used herein, the term “peptide stapling” or “hydrocarbon stapling” includes the joining of two (e.g., at least one pair of) double bond-containing side-chains, triple bond-containing side-chains, or double bond-containing and triple bond-containing side chain, which can be present in a polypeptide chain, using any number of reaction conditions and/or catalysts to facilitate such a reaction, to provide a singly “stapled” polypeptide. The term “multiply stapled” polypeptides refers to those polypeptides containing more than one individual staple, and can contain two, three, or more independent staples of various spacings and compositions. The term “peptide stitching,” as used herein, refers to multiple and tandem “stapling” events in a single polypeptide chain to provide a “stitched” (e.g., tandem or multiply stapled) polypeptide, in which two staples, e.g., are linked to a common residue. Peptide stitching is disclosed in U.S. Pat. Nos. 8,592,377 and 9,079,970, which are both hereby incorporated by reference in their entirety. In some instances, staples, as used herein, can retain the unsaturated bond or can be reduced (e.g., as mentioned below in the stitching paragraph description).
While many peptide staples have all hydrocarbon cross-links, other type of cross-links or staples can be used. For example, triazole-containing (e.g, 1, 4 triazole or 1, 5 triazole) crosslinks can be used (see, e.g., Kawamoto et al., J. Med. Chem. 55:1137-1146, 2012; WO 2010/060112).
Stapling of a peptide using an all-hydrocarbon cross-link has been shown to help maintain its native conformation and/or secondary structure, particularly under physiologically relevant conditions (see, e.g., Schafmiester et al., J. Am. Chem. Soc. 122:5891-5892, 2000; Walensky et al., Science 305:1466-1470, 2004).
Stapling the polypeptide herein by an all-hydrocarbon crosslink predisposed to have an alpha-helical secondary structure can constrain the polypeptide to its native alpha-helical conformation. The constrained secondary structure can, for example, increase the peptide's resistance to proteolytic cleavage, can increase the peptide's thermal stability, can increase the peptide's hydrophobicity, can allow for better penetration of the peptide into the target cell's membrane (e.g., through an energy-dependent transport mechanism, such as pinocytosis), and/or can lead to an improvement in the peptide's biological activity relative to the corresponding uncross-linked (e.g., “unstitched” or “unstapled”) peptide.
While at least two amino acids are required to support an internal cross-link (e.g., a staple), additional pairs of internally cross-linked amino acids can be included in a internally cross-linked polypeptide or modified polypeptide, e.g., to support additional internal cross-links (e.g., staples). For example, internally cross-linked polypeptides and modified polypeptides can include 1, 2, 3, 4, 5, or more staples. Internally cross-linked polypeptides and modified polypeptides (e.g., stapled and/or stitched peptides) are generally referred to herein as “stabilized alpha-helix of BCL-2 domains” (SAHBs).
Alternatively or in addition, internally cross-linked polypeptides and modified polypeptides can include three internally cross-linked or stitched amino acids, e.g., yielding two staples arising from a common origin. A peptide stitch includes at least three internally cross-linked amino acids, wherein the middle of the three amino acids (referred to here as the core or central amino acid and shown as “i”) forms an internal cross-link (between alpha carbons) with each of the two flanking modified amino acids. The alpha carbon of the core amino acid has side chains that are internal cross-links to the alpha carbons of other amino acids in the peptide, which can be saturated or not saturated. Amino acids cross-linked to the core amino acid can be separated from the core amino acid in either direction by 2, 3, or 6 amino acids (e.g. i, i−3, i, i−4, i, i−7 or, i, i+3, i, i+4, i, i+7), where “i” is the core amino acid). The number of amino acids on either side of the core (e.g., between the core amino acid and an amino acid cross-linked to the core) can be the same or different. In some instances, a stitch can include 3, 4, 5, or more internally cross-linked amino acids. In some instances, internally cross-linked polypeptides and modified polypeptides can include 1, 2, 3, 4, 5, or more stitches.
In some aspects, internally cross-linked polypeptides or modified polypeptides described herein can include a combination of at least one (e.g., 1, 2, 3, 4, or 5) staple and at least one (e.g., 1, 2, 3, 4, or 5) stitch.
Internal cross-links (e.g., staples and/or stitches) can be positioned on amino acids within an internally cross-linked polypeptide or modified polypeptide to conserve the structural relationship of amino acids in the binding or the interacting face of the peptide (e.g., to preserve the binding interface of a peptide). Alternatively, staples can placed on the interacting face as long as binding affinity or activity is not altered. The “interacting face” of the internally cross-linked polypeptides described herein includes those amino acid residues of the alpha helix that interact (e.g., interact specifically or bind specifically) with a BAX protein. In some aspects, the staple or staples can be placed such that they partially or completely engage the target and enhance binding activity. For example, staples or stitches can be placed to conserve the structural relationship of amino acids in an interaction face of the internally cross-stitched polypeptide or modified polypeptide. Such internal cross-links can include: one or more staples; one or more stitches; and/or a combination of one or more staples with one or more stitches.
Selection of amino acids for modification (e.g., to support an internal cross-link) can also be facilitated by staple scanning. The term “staple scan” refers to the synthesis of a library of stapled peptides whereby the location of the i and i+3; i and i+4; and i and i+7 single and multiple staple, or stitches, are positioned sequentially down the length of the peptide sequence, sampling all possible positions, to identify desired or optimal properties and activities for the stapled or stitched constructs.
In some aspects, the tethers, e.g., hydrocarbon staples are used to stabilize structures other than helices. In such cases, the ends of the tethers can be placed at intervals other than at i, i+3, i+4, and i+7.
Structurally constrained peptides and the like are understood to include modified peptides having any (i.e., at least one) chemical modification, e.g., mutation of the original or native sequence with a natural or non-natural amino acid; chemical modification to incorporate a molecular tether, chemical modification to promote the formation of a disulfide bridge, etc., such that the structurally constrained peptide adopts a more limited number of structures than the unmodified peptide. A structurally constrained peptide can include 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or more mutations as compared to the native, wild-type sequence. For example, molecular tethers can include hydrocarbon staples to promote the formation of stable helical structures, especially alpha-helical and 310 helix structures, or kinks depending on the positions of the ends of the tethers and the lengths of the tethers. Natural or non-natural amino acids can be employed to promote kinks (e.g., bends in the structure as defined by the variable angles between the two adjoining structures) or other preferred confirmations. For example, the natural amino acid proline can induce a kink in a peptide due to the structure of the amino acid R group and the lack of a hydrogen-bond donor. Non-natural amino acids, particularly those having large and/or charged R groups, or N-methylated amides, N-substituted glycines, cyclic alpha, alpha-disubstitution, cyclic N,N-disubstitution, and beta-amino acids can promote specific, desired confirmations. It is understood that a population of “structurally constrained” peptides in solution can not all have the desired confirmation all of the time. Instead, in a population of structurally constrained peptides in solution, the desired confirmation is present at least about 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, or more of the time than the native or original peptide sequence in solution prior to chemical modification. The structure of a population of peptides in solution can be determined by various methods known to those of skill in the art including, but not limited to, circular dichroism and NMR spectroscopy. X-ray crystallography can be applied to determine the structure of a constrained peptide when packed in the form of a crystal.
Suitable tethers are described herein and in U.S. Patent Application Publication No. 2005/0250680, U.S. Pat. No. 8,592,377, U.S. Patent Application Publication No. 2011/0318352, WO 2009/108261, and WO 2010/148335, each of which are herein incorporated by reference in their entireties.
Amino acid side chains suitable for use in the internally cross-linked polypeptides and modified polypeptides disclosed herein are known in the art. For example, suitable amino acid side chains include methyl (as the alpha-amino acid side chain for alanine is methyl), 4-hydroxyphenylmethyl (as the alpha-amino acid side chain for tyrosine is 4-hydroxyphenylmethyl) and thiomethyl (as the alpha-amino acid side chain for cysteine is thiomethyl), etc. A “terminally unsaturated amino acid side chain” refers to an amino acid side chain bearing a terminal unsaturated moiety, such as a substituted or unsubstituted, double bond (e.g., olefinic) or triple bond (e.g., acetylenic), that participates in cross-linking reaction with other terminal unsaturated moieties in the polypeptide chain. In certain aspects, a “terminally unsaturated amino acid side chain” is a terminal olefinic amino acid side chain. In certain aspects, a “terminally unsaturated amino acid side chain” is a terminal acetylenic amino acid side chain. In certain aspects, the terminal moiety of a “terminally unsaturated amino acid side chain” is not further substituted.
As noted above, an internal tether or cross-link can extend across the length of one helical turn (i.e., about 3.4 amino acids (i.e., i, i+3, or i, i+4) or two helical turns (i.e., about 7 amino acids (i.e., i, i+7). Accordingly, amino acids positioned at i and i+3; i and i+4; or i and i+7 of SEQ ID NO:2 or 3 are ideal candidates for chemical modification and cross-linking. Thus, e.g., where a peptide has the sequence . . . Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, Xaa6, Xaa7, Xaa8, Xaa9 . . . (wherein “ . . . ” indicates the optional presence of additional amino acids), cross-links between Xaa1 and Xaa4, or between Xaa1 and Xaa5, or between Xaa1 and Xaa8 are useful, as are cross-links between Xaa2 and Xaa5, or between Xaa2 and Xaa6, or between Xaa2 and Xaa9, etc.
Internally cross-linked polypeptides and modified polypeptides can include more than one crosslink within the polypeptide sequence to either further stabilize the sequence or facilitate the stabilization of longer polypeptide stretches. If the polypeptides are too long to be readily synthesized in one part, independently synthesized, internally cross-linked polypeptides or modified polypeptides can be conjoined by a technique called native chemical ligation (see, e.g., Bang, et al., J. Am. Chem. Soc. 126:1377-1383, 2004). Alternately, large peptides are routinely synthesized using a convergent approach, whereby fully protected fragments are specifically and sequentially reacted to form the full length desired product, after final deprotection, such as in the industrial synthesis of Fuzeon.
The invention features a modified polypeptide of Formula (I),
or a pharmaceutically acceptable salt thereof,
wherein;
each R1 and R2 are independently H or a C1 to C10 alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heteroarylalkyl, or heterocyclylalkyl;
R3 is alkylene, alkenylene, or alkynylene (e.g., a C6, C7, C8, C11, C12, or C13 alkylene), or [R4′-K-R4]n; each of which is substituted with 0-6 R5;
R4 and R4′ are independently alkylene, alkenylene, or alkynylene (e.g., each are independently a C1, C2, C3, C4, C5, C6, C7, C8, C9, or C10 alkylene, alkenylene or alkynylene);
R5 is halo, alkyl, OR6, N(R6)2, SR6, SORE, SO2R6, CO2R6, R6, a fluorescent moiety, or a radioisotope;
K is O, S, SO, SO2, CO, CO2, CONR6,
aziridine, episulfide, diol, or amino alcohol;
R6 is H, alkyl, or a therapeutic agent;
n is 2, 3, 4, or 6;
x is an integer from 2-10;
w and y are each independently an integer from 0-100;
z is an integer from 1-10 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10); and
each Xaa is independently an amino acid (e.g., one of the 20 naturally occurring amino acids or any naturally occurring non-naturally occurring amino acid);
wherein the polypeptide comprises at least 8 (e.g., at least 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18) contiguous amino acids of SEQ ID NO: 2 or 3 or a variant thereof (e.g., one, two, three, four, five, six, or seven amino acid substitutions), or another polypeptide sequence described herein, except that: (a) within the 8 contiguous (e.g., 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18) amino acids of SEQ ID NO: 2 or 3 the side chains of at least one pair (e.g., one, two, three, or four pairs) of amino acids separated by 2 to 6 amino acids (e.g., 2, 3, or 6 amino acids) is replaced by the linking group, R3, which connects the alpha carbons of the pair of amino acids as depicted in Formula I; and (b) the alpha carbon of the first of the pair of amino acids is substituted with R1 as depicted in Formula I and the alpha carbon of the second of the pair of amino acids is substituted with R2 as depicted in Formula I.
In another aspect, the invention features a modified polypeptide of Formula (II),
or a pharmaceutically acceptable salt thereof,
wherein;
each R1 and R2 are independently H or a C1 to C10 alkyl, alkenyl, alkynyl, arylalkyl, cycloalkylalkyl, heteroarylalkyl, or heterocyclylalkyl;
R3 is alkylene, alkenylene, or alkynylene (e.g., a C6, C7, C8, C11, C12, or C13 alkylene) or [R4′-K-R4]n; each of which is substituted with 0-6 R5;
R4 and R4′ are independently alkylene, alkenylene, or alkynylene (e.g., each are independently a C1, C2, C3, C4, C5, C6, C7, C8, C9, or C10 alkylene, alkenylene, or alkynylene);
R5 is halo, alkyl, OR6, NHR6, N(R6)2, SR6, SOR6, SO2R6, CO2R6, R6, a fluorescent moiety, or a radioisotope;
K is O, S, SO, SO2, CO, CO2, CONR6,
aziridine, episulfide, diol, amino alcohol, or diamine;
R6 is H, alkyl, or a therapeutic agent;
n is 2, 3, 4, 5, or 6;
x is an integer from 2-10;
w and y are each independently an integer from 0-100;
z is an integer from 1-10 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10); and
each Xaa is independently an amino acid (e.g., one of the 20 naturally occurring amino acids or any naturally occurring non-naturally occurring amino acid);
R7 is selected from: a) PEG, b) a tat protein, c) an affinity label, d) a targeting moiety, e) a fatty acid-derived acyl group, f) a biotin moiety, g) a fluorescent probe (e.g., fluorescein or rhodamine) linked via, e.g., a thiocarbamate or carbamate linkage, or h) a sequence of 2, 3, 4, 5, 6, 6, 8, or 9 contiguous Arg;
R8 is H, OH, NH2, NHR8a, or NR8aR8b;
wherein the polypeptide comprises at least 8 contiguous amino acids (e.g., 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18) of SEQ ID NO: 2 or 3 or a variant thereof (e.g., one, two, three, four, five, six, or seven amino acid substitutions), or another polypeptide sequence described herein except that: (a) within the at least 8 (e.g., 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18) contiguous amino acids of any one of SEQ ID NO: 2 or 3, the side chains of at least one (e.g., 2, 3, 4, or 5) pair of amino acids separated by 2 to 6 amino acids (e.g., 2, 3, or 6 amino acids) is replaced by the linking group, R3, which connects the alpha carbons of the pair of amino acids as depicted in Formula II; and (b) the alpha carbon of the first of the pair of amino acids is substituted with R1 as depicted in Formula II and the alpha carbon of the second of the pair of amino acids is substituted with R2 as depicted in Formula II.
In the case of Formula I or Formula II, the following aspects are among those disclosed.
In cases where x=2 (i.e., i+3 linkage), R3 can be a C7 alkylene or alkenylene. Where it is an alkenylene, there can one or more double bonds. In cases where x=6 (i.e., i+4 linkage), R3 can be a C11, C12, or C13 alkylene or alkenylene. Where it is an alkenylene, there can one or more double bonds. In cases where x=3 (i.e., i+4 linkage), R3 can be a C8 alkylene or alkenylene. Where it is an alkenylene, there can one or more double bonds.
In certain instances, the two alpha, alpha-disubstituted stereocenters (alpha carbons) are both in the R configuration or S configuration (e.g., i, i+4 cross-link), or one stereocenter is R and the other is S (e.g., i, i+7 cross-link). Thus, where Formula I is depicted as
the C′ and C″ disubstituted stereocenters can both be in the R configuration or they can both be in the S configuration, for example, when x is 3. When x is 6, the C′ disubstituted stereocenter is in the R configuration and the C″ disubstituted stereocenter is in the S configuration or the C′ disubstituted stereocenter is in the S configuration and the C″ disubstituted stereocenter is in the R configuration. The R3 double bond can be in the E or Z stereochemical configuration. Similar configurations are possible for the carbons in Formula II corresponding to C′ and C″ in the formula depicted immediately above.
In some instances R3 is [R4-K-R4′]n; and R4 and R4′ are independently alkylene, alkenylene, or alkynylene (e.g., each are independently a C1, C2, C3, C4, C5, C6, C7, C8, C9, or C10 alkylene, alkenylene or alkynylene).
In some instances of any of the modified polypeptides described herein, the modified polypeptide includes an amino acid sequence which, in addition to the amino acids side chains that are replaced by a cross-link, have 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 amino acid changes (e.g., conservative amino acid changes) in SEQ ID NO: 2 or 3.
The tether can include an alkyl, alkenyl, or alkynyl moiety (e.g., C6, C8, or C11 alkyl, a C6, C8, or C11 alkenyl, or C5, C8, or C11 alkynyl). The tethered amino acid can be alpha disubstituted (e.g., C1-C3 or methyl). [Xaa]y and [Xaa]w are peptides that can independently comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18 contiguous amino acids of SEQ ID NO: 3 and [Xaa]x is a peptide that can comprise between 2 to 7 (e.g., 2, 3, 6, or 7) contiguous amino acids of acids of SEQ ID NO: 2 or 3.
The internally cross-linked polypeptides and modified polypeptides can contain one or more asymmetric centers and thus occur as racemates and racemic mixtures, single enantiomers, individual diastereomers, and diastereomeric mixtures and geometric isomers (e.g., Z or cis and E or trans) of any olefins present. For example, internally cross-linked polypeptides or modified polypeptides disclosed herein can exist in particular geometric or stereoisomeric forms, including, for example, cis- and trans-isomers, R- and S-enantiomers, diastereomers, (D)-isomers, (L)-isomers, the racemic mixtures thereof, and other mixtures thereof. Enantiomers can be free (e.g., substantially free) of their corresponding enantiomer, and/or can also be optically enriched. “Optically enriched,” as used herein, means that the compound is made up of a significantly greater proportion of one enantiomer. In certain aspects substantially free means that a composition contains at least about 90% by weight of a preferred enantiomer. In other aspects the compound is made up of at least about 95%, 98%, or 99% by weight of a preferred enantiomer. Preferred enantiomers can be isolated from racemic mixtures using techniques known in the art, including, but not limited to, for example, chiral high pressure liquid chromatography (HPLC) and the formation and crystallization of chiral salts or prepared by asymmetric syntheses (see, e.g., Jacques, et al., Enantiomers, Racemates and Resolutions (Wiley Interscience, New York, 1981); Wilen, S. H. et al., Tetrahedron 33:2725 (1977); Eliel, E X, Stereochemistry of Carbon Compounds (McGraw-Hill, N Y, 1962); Wilen, S. H., Tables of Resolving Agents and Optical Resolutions p. 268 (EX. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, Ind., 1972). All such isomeric forms of these internally cross-linked polypeptides and modified polypeptides are expressly included in the present invention.
The internally cross-linked polypeptides or modified polypeptides can also be represented in multiple tautomeric forms, in such instances, the invention expressly includes all tautomeric forms of the compounds described herein (e.g., isomers in equilibrium (e.g., keto-enol), wherein alkylation at multiple sites can yield regioisomers), regioisomers, and oxidation products of the internally cross-linked polypeptides or modified polypeptides disclosed herein (the invention expressly includes all such reaction products). All such isomeric forms of such internally cross-linked polypeptides or modified polypeptides are included, as are all crystal forms.
The symbol “,” when used as part of a molecular structure, refers to a single bond or a trans or cis double bond.
The term “halo” refers to any radical of fluorine, chlorine, bromine, or iodine. The term “alkyl” refers to a hydrocarbon chain that can be a straight chain or branched chain, containing the indicated number of carbon atoms. For example, C1-C10 indicates that the group can have from 1 to 10 (inclusive) carbon atoms in it. In the absence of any numerical designation, “alkyl” is a chain (straight or branched) having 1 to 20 (inclusive) carbon atoms in it. The term “alkylene” refers to a divalent alkyl (i.e., —R—).
The term “alkenyl” refers to a hydrocarbon chain that can be a straight chain or branched chain having one or more carbon-carbon double bonds in either Z or E geometric configurations. The alkenyl moiety contains the indicated number of carbon atoms. For example, C2-C10 indicates that the group can have from 2 to 10 (inclusive) carbon atoms in it. The term “lower alkenyl” refers to a C2-C8 alkenyl chain. In the absence of any numerical designation, “alkenyl” is a chain (straight or branched) having 2 to 20 (inclusive) carbon atoms in it.
The term “alkynyl” refers to a hydrocarbon chain that can be a straight chain or branched chain having one or more carbon-carbon triple bonds. The alkynyl moiety contains the indicated number of carbon atoms. For example, C2-C10 indicates that the group can have from 2 to 10 (inclusive) carbon atoms in it. The term “lower alkynyl” refers to a C2-C8 alkynyl chain. In the absence of any numerical designation, “alkynyl” is a chain (straight or branched) having 2 to 20 (inclusive) carbon atoms in it.
The term “aryl” refers to a 6-carbon monocyclic or 10-carbon bicyclic aromatic ring system wherein 0, 1, 2, 3, 4, or 5 atoms of each ring can be substituted by a substituent. Examples of aryl groups include phenyl, naphthyl, and the like. The term “arylalkyl” or the term “aralkyl” refers to alkyl substituted with an aryl. The term “arylalkoxy” refers to an alkoxy substituted with aryl.
The term “cycloalkyl” as employed herein includes saturated and partially unsaturated cyclic hydrocarbon groups having 3 to 12 carbons, preferably 3 to 8 carbons, and more preferably 3 to 6 carbons, wherein the cycloalkyl group additionally can be optionally substituted. Preferred cycloalkyl groups include, without limitation, cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cyclohexadienyl, cycloheptyl, cycloheptadienyl, cycloheptatrienyl, cyclooctyl, cyclooctenyl, cyclooctadienyl, cyclooctatrienyl, and cyclooctynyl.
The term “heteroaryl” refers to an aromatic 5-8 membered monocyclic, 8-12 membered bicyclic, or 11-14 membered tricyclic ring system having 1-3 heteroatoms if monocyclic, 1-6 heteroatoms if bicyclic, or 1-9 heteroatoms if tricyclic, said heteroatoms selected from O, N, or S (e.g., carbon atoms and 1-3, 1-6, or 1-9 heteroatoms of N, O, or S if monocyclic, bicyclic, or tricyclic, respectively), wherein 0, 1, 2, 3, or 4 atoms of each ring can be substituted by a substituent. Examples of heteroaryl groups include pyrrolyl, pyridyl, furyl or furanyl, imidazolyl, 1,2,3-triazolyl, 1,2,4-triazolyl, benzimidazolyl, pyridazyl, pyrimidyl, thiophenyl, quinolinyl, indolyl, thiazolyl, oxazolyl, isoxazolyl, and the like. The term “heteroarylalkyl” or the term “heteroaralkyl” refers to an alkyl substituted with a heteroaryl. The term “heteroarylalkoxy” refers to an alkoxy substituted with heteroaryl.
The term “heterocyclyl” refers to a nonaromatic 5-8 membered monocyclic, 8-12 membered bicyclic, or 11-14 membered tricyclic ring system having 1-3 heteroatoms if monocyclic, 1-6 heteroatoms if bicyclic, or 1-9 heteroatoms if tricyclic, said heteroatoms selected from O, N, or S (e.g., carbon atoms and 1-3, 1-6, or 1-9 heteroatoms of N, O, or S if monocyclic, bicyclic, or tricyclic, respectively), wherein 0, 1, 2 or 3 atoms of each ring can be substituted by a substituent. Examples of heterocyclyl groups include piperazinyl, pyrrolidinyl, dioxanyl, aziridinyl, oxiryl, thiiryl, morpholinyl, tetrahydrofuranyl, and the like.
The term “substituents” refers to a group “substituted” on an alkyl, cycloalkyl, aryl, heterocyclyl, or heteroaryl group at any atom of that group. Suitable substituents include, without limitation, halo, hydroxy, mercapto, oxo, nitro, haloalkyl, alkyl, alkaryl, aryl, aralkyl, alkoxy, thioalkoxy, aryloxy, amino, alkoxycarbonyl, amido, carboxy, alkanesulfonyl, alkylcarbonyl, azido, and cyano groups.
In some instances, the hydrocarbon tethers (i.e., cross-links) described herein can be further manipulated. In one instance, a double bond of a hydrocarbon alkenyl tether (e.g., as synthesized using a ruthenium-catalyzed ring closing metathesis (RCM)) can be oxidized (e.g., via epoxidation or dihydroxylation) to provide one of compounds below.
Either the epoxide moiety or one of the free hydroxyl moieties can be further functionalized. For example, the epoxide can be treated with a nucleophile, which provides additional functionality that can be used, for example, to attach a tag (e.g., a radioisotope or fluorescent tag). The tag can be used to help direct the compound to a desired location in the body or track the location of the compound in the body. Alternatively, an additional therapeutic agent can be chemically attached to the functionalized tether (e.g., a cytoprotective agent). Such derivatization can alternatively be achieved by synthetic manipulation of the amino or carboxy-terminus of the internally cross-linked polypeptide or modified polypeptide, or via the amino acid side chain. Other agents can be attached to the functionalized tether, e.g., an agent that facilitates entry of the internally cross-linked polypeptide or modified polypeptide into cells.
While hydrocarbon tethers (cross-links) have been described, other tethers (cross-links) are also envisioned. For example, the tether can include one or more of an ether, thioether, ester, amine, or amide moiety. In some cases, a naturally-occurring amino acid side chain can be incorporated into the tether. For example, a tether can be coupled with a functional group such as the hydroxyl in serine, the thiol in cysteine, the primary amine in lysine, the acid in aspartate or glutamate, or the amide in asparagine or glutamine. Accordingly, it is possible to create a tether using naturally-occurring amino acids rather than using a tether that is made by coupling two non-naturally occurring amino acids. It is also possible to use a single non-naturally occurring amino acid together with a naturally occurring amino acid.
It is further envisioned that the length of the tether (cross-link) can be varied. For instance, a shorter length of tether can be used where it is desirable to provide a relatively high degree of constraint on the secondary alpha-helical structure, whereas, in some instances, it is desirable to provide less constraint on the secondary alpha-helical structure, and thus a longer tether can be desired.
Additionally, while examples of tethers (cross-links) spanning from amino acids i to i+3, i to i+4; and i to i+7 have been described in order to provide a tether that is primarily on a single face of the alpha helix, the tethers can be synthesized to span any combinations of numbers of amino acids (e.g., i to i+7).
In some instances, alpha-disubstituted amino acids are used in the internally cross-linked polypeptide or modified polypeptide to improve the stability of the alpha-helical secondary structure. However, alpha-disubstituted amino acids are not required, and instances using mono-alpha substituents (e.g., in the tethered amino acids) are also envisioned.
In some instances, it can be useful to replace an amino acid on the interacting face of SEQ ID NO: 2 or 3 with another amino acid, e.g., Ala. Such inactive internally cross-linked polypeptides or modified polypeptides can be useful, e.g., as negative controls.
The internally cross-linked polypeptides or modified polypeptides can include a drug, a toxin, a derivative of polyethylene glycol; a second polypeptide; a carbohydrate, etc. Where a polymer or other agent is linked to the internally cross-linked polypeptide or modified polypeptide it can be desirable for the composition containing these internally cross-linked polypeptides or modified polypeptides to be substantially homogeneous.
The addition of polyethelene glycol (PEG) molecules can improve the pharmacokinetic and pharmacodynamic properties of the modified polypeptide or internally cross-linked polypeptide. For example, PEGylation can reduce renal clearance and can result in a more stable plasma concentration. PEG is a water soluble polymer and can be represented as linked to the polypeptide as formula:
XO—(CH2CH2O)n—CH2CH2—Y
where n is 2 to 10,000 and X is H or a terminal modification, e.g., a C1-4 alkyl; and Y is an amide, carbamate, or urea linkage to an amine group (including but not limited to, the epsilon amine of lysine or the N-terminus) of the internally cross-linked polypeptide or modified polypeptide. Y can also be a maleimide linkage to a thiol group (including but not limited to, the thiol group of cysteine). Other methods for linking PEG to a polypeptide, directly or indirectly, are known to those of ordinary skill in the art. The PEG can be linear or branched. Various forms of PEG including various functionalized derivatives are commercially available.
PEG having degradable linkages in the backbone can be used. For example, PEG can be prepared with ester linkages that are subject to hydrolysis. Conjugates having degradable PEG linkages are described in WO 99/34833; WO 99/14259, and U.S. Pat. No. 6,348,558.
In certain aspects, macromolecular polymer (e.g., PEG) is attached to a modified polypeptide or internally cross-linked polypeptide described herein through an intermediate linker. In certain aspects, the linker is made up of from 1 to 50 (e.g., 5, 15, 20, 25) amino acids linked by peptide bonds, wherein the amino acids are selected from the 20 naturally-occurring amino acids. Some of these amino acids can be glycosylated, as is well understood by those in the art. In other aspects, the 1 to 50 amino acids are selected from glycine, alanine, proline, asparagine, glutamine, and lysine. In other aspects, a linker is made up of a majority of amino acids that are sterically unhindered, such as glycine and alanine. In other aspects, a linker includes glycine and/or serine (e.g., G4S (SEQ ID NO: 33)). Non-peptide linkers are also possible. For example, alkyl linkers such as —NH(CH2)nC(O)—, wherein n=2-20 can be used. These alkyl linkers can further be substituted by any non-sterically hindering group such as lower alkyl (e.g., C1-C6), lower acyl, halogen (e.g., C1, Br), CN, NH2, phenyl, etc. U.S. Pat. No. 5,446,090 describes a bifunctional PEG linker and its use in forming conjugates having a peptide at each of the PEG linker termini.
The internally cross-linked polypeptides or modified polypeptides can also be modified, e.g., to further facilitate cellular uptake or increase in vivo stability, in some aspects. For example, acylating or PEGylating a peptidomimetic facilitates cellular uptake, increases bioavailability, increases blood circulation, alters pharmacokinetics, decreases immunogenicity, and/or decreases the needed frequency of administration.
In some aspects, the internally cross-linked polypeptides or modified polypeptides disclosed herein have an enhanced ability to penetrate cell membranes (e.g., relative to non-stapled peptides).
Methods of synthesizing the internally cross-linked polypeptides or modified polypeptides described herein are known in the art. Nevertheless, the following exemplary method can be used. It will be appreciated that the various steps can be performed in an alternate sequence or order to give the desired compounds. Synthetic chemistry transformations and protecting group methodologies (protection and deprotection) useful in synthesizing the internally cross-linked polypeptides and modified polypeptides described herein are known in the art and include, e.g., those such as described in R. Larock, Comprehensive Organic Transformations, VCH Publishers (1989); T. W. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 3d. Ed., John Wiley and Sons (1999); L. Fieser and M. Fieser, Fieser and Fieser's Reagents for Organic Synthesis, John Wiley and Sons (1994); and L. Paquette, ed., Encyclopedia of Reagents for Organic Synthesis, John Wiley and Sons (1995), and subsequent editions thereof.
The internally cross-linked polypeptides and modified polypeptides of this invention can be made by chemical synthesis methods, which are well known to the ordinarily skilled artisan. See, e.g., Fields et al., Chapter 3 in Synthetic Peptides: A User's Guide, ed. Grant, W. H. Freeman & Co., New York, N.Y., 1992, p. 77. Hence, peptides can be synthesized using the automated Merrifield techniques of solid phase synthesis with the α-NH2 protected by either t-Boc or Fmoc chemistry using side chain protected amino acids on, e.g., an Applied Biosystems Peptide Synthesizer Model 430A or 431.
One manner of making of the cross-linked polypeptides or modified polypeptides described herein is using solid phase peptide synthesis (SPPS). The C-terminal amino acid is attached to a cross-linked polystyrene resin via an acid labile bond with a linker molecule. This resin is insoluble in the solvents used for synthesis, making it relatively simple and fast to wash away excess reagents and by-products. The N-terminus is protected with the Fmoc group, which is stable in acid, but removable by base. Any side chain functional groups are protected with base stable, acid labile groups.
Longer peptides can be made by conjoining individual synthetic peptides using native chemical ligation. Alternatively, the longer synthetic peptides can be synthesized by well-known recombinant DNA techniques. Such techniques are provided in well-known standard manuals with detailed protocols. To construct a gene encoding a peptide of this invention, the amino acid sequence is reverse translated to obtain a nucleic acid sequence encoding the amino acid sequence, preferably with codons that are optimum for the organism in which the gene is to be expressed. Next, a synthetic gene is made, typically by synthesizing oligonucleotides which encode the peptide and any regulatory elements, if necessary. The synthetic gene is inserted in a suitable cloning vector and transfected into a host cell. The peptide is then expressed under suitable conditions appropriate for the selected expression system and host. The peptide is purified and characterized by standard methods. The peptides can be made in a high-throughput, combinatorial fashion, e.g., using a high-throughput multiple channel combinatorial synthesizer available from Advanced Chemtech.
One or more peptide bonds can be replaced, e.g., to increase physiological stability of the internally cross-linked polypeptide or modified polypeptide, by: a retro-inverso bonds (C(O)—NH); a reduced amide bond (NH—CH2); a thiomethylene bond (S—CH2 or CH2—S); an oxomethylene bond (O—CH2 or CH2—O); an ethylene bond (CH2—CH2); a thioamide bond (C(S)—NH); a trans-olefin bond (CH═CH); a fluoro-substituted trans-olefin bond (CF═CH); a ketomethylene bond (C(O)—CHR) or CHR—C(O), wherein R is H or CH3; and a fluoro-ketomethylene bond (C(O)—CFR or CFR—C(O), wherein R is H, F, or CH3.
The internally cross-linked polypeptides or modified polypeptides can be further modified by one or more of: acetylation, amidation, biotinylation, cinnamoylation, farnesylation, fluoresceination, formylation, myristoylation, palmitoylation, phosphorylation (Ser, Tyr, or Thr), stearoylation, succinylation, and sulfurylation. As indicated above, internally cross-linked polypeptides and modified polypeptides can be conjugated to, for example, polyethylene glycol (PEG); alkyl groups (e.g., C1-C20 straight or branched alkyl groups); fatty acid radicals; and combinations thereof.
α, α-Disubstituted non-natural amino acids containing olefinic side chains of varying length can be synthesized by known methods (see, e.g., Williams et al., J. Am. Chem. Soc. 113:9276, 1991; Schafmeister et al., J. Am. Chem Soc. 122:5891, 2000; Bird et al., Methods Enzymol., 446:369, 2008; Bird et al, Current Protocols in Chemical Biology, 2011). For internally cross-linked polypeptides or modified polypeptides, where an i linked to i+7 staple is used (two turns of the helix stabilized), either one S5 amino acid and one R8 is used, or one S8 amino acid and one R5 amino acid is used. R8 is synthesized using the same route, except that the starting chiral auxillary confers the R-alkyl-stereoisomer. Also, 8-iodooctene is used in place of 5-iodopentene. Inhibitors are synthesized on a solid support using solid-phase peptide synthesis (SPPS) on MBHA resin (see, e.g., WO 2010/148335).
Fmoc-protected α-amino acids (other than the olefinic amino acids Fmoc-S5—OH, Fmoc-R8—OH, Fmoc-R8—OH, Fmoc-S8—OH, and Fmoc-R5—OH), 2-(6-chloro-1-H-benzotriazole-1-yl)-1,1,3,3-tetramethylaminium hexafluorophosphate (HCTU), and Rink Amide MBHA are commercially available from, e.g., Novabiochem (San Diego, Calif.). Dimethylformamide (DMF), N-methyl-2-pyrrolidinone (NMP), N,N-diisopropylethylamine (DIEA), trifluoroacetic acid (TFA), 1,2-dichloroethane (DCE), fluorescein isothiocyanate (FITC), and piperidine are commercially available from, e.g., Sigma-Aldrich. Olefinic amino acid synthesis is reported in the art (Williams et al., Org. Synth., 80:31, 2003).
In some instances, the internally cross-linked polypeptides and modified polypeptides can include a detectable label. As used herein, a “label” refers to a moiety that has at least one element, isotope, or functional group incorporated into the moiety which enables detection of the peptide (e.g., internally cross-linked polypeptide or modified polypeptide) to which the label is attached. Labels can be directly attached (i.e., via a bond) or can be attached by a linker (e.g., such as, for example, a cyclic or acyclic, branched or unbranched, substituted or unsubstituted alkylene; cyclic or acyclic, branched or unbranched, substituted or unsubstituted alkenylene; cyclic or acyclic, branched or unbranched, substituted or unsubstituted alkynylene; cyclic or acyclic, branched or unbranched, substituted or unsubstituted heteroalkylene; cyclic or acyclic, branched or unbranched, substituted or unsubstituted heteroalkenylene; cyclic or acyclic, branched or unbranched, substituted or unsubstituted heteroalkynylene; substituted or unsubstituted arylene; substituted or unsubstituted heteroarylene; or substituted or unsubstituted acylene, or any combination thereof, which can make up a linker). Labels can be attached to an internally cross-linked polypeptide or modified polypeptide at any position that does not interfere with the biological activity or characteristic of the inventive internally cross-linked polypeptide or modified polypeptide that is being detected.
Labels can include: labels that contain isotopic moieties, which can be radioactive or heavy isotopes, including, but not limited to, 2H, 3H, 13C, 14C, 15N, 31P, 32P, 35S, 67Ga, 99mTc (Tc-99m), 111In, 123I, 125I, 169Yb, and 186Re; labels that include immune or immunoreactive moieties, which can be antibodies or antigens, which can be bound to enzymes (e.g., such as horseradish peroxidase); labels that are colored, luminescent, phosphorescent, or include fluorescent moieties (e.g., such as the fluorescent label FITC); labels that have one or more photoaffinity moieties; labels that have ligand moieties with one or more known binding partners (such as biotin-streptavidin, FK506-FKBP, etc.).
In some instances, labels can include one or more photoaffinity moieties for the direct elucidation of intermolecular interactions in biological systems. A variety of known photophores can be employed, most relying on photoconversion of diazo compounds, azides, or diazirines to nitrenes or carbenes (see, e.g., Bayley, H., Photogenerated Reagents in Biochemistry and Molecular Biology (1983), Elsevier, Amsterdam, the entire contents of which are incorporated herein by reference). In certain aspects of the invention, the photoaffinity labels employed are o-, m- and p-azidobenzoyls, substituted with one or more halogen moieties, including, but not limited to 4-azido-2,3,5,6-tetrafluorobenzoic acid.
Labels can also be or can serve as imaging agents. Exemplary imaging agents include, but are not limited to, those used in positron emissions tomography (PET), computer assisted tomography (CAT), single photon emission computerized tomography, X-ray, fluoroscopy, and magnetic resonance imaging (MRI); anti-emetics; and contrast agents. Exemplary diagnostic agents include but are not limited to, fluorescent moieties, luminescent moieties, magnetic moieties; gadolinium chelates (e.g., gadolinium chelates with DTPA, DTPA-BMA, DOTA, and HP-DO3A), iron chelates, magnesium chelates, manganese chelates, copper chelates, chromium chelates, iodine-based materials useful for CAT and x-ray imaging, and radionuclides. Suitable radionuclides include, but are not limited to, 123I, 125I, 130I, 131I, 133I, 135I, 47Sc, 72As, 72Se, 90Y, 88Y, 97Ru, 100Pd, 101mRh, 119Sb, 121Ba, 197Hg, 211At, 212Bi, 212Pb, 109Pd, 111In, 67Ga, 68Ga, 67Cu, 75Br, 77Br, 99mTc, 14C, 13N, 15O, 32P, 33P, and 18F.
Fluorescent and luminescent moieties include, but are not limited to, a variety of different organic or inorganic small molecules commonly referred to as “dyes,” “labels,” or “indicators.” Examples include, but are not limited to, fluorescein, rhodamine, acridine dyes, Alexa dyes, cyanine dyes, etc. Fluorescent and luminescent moieties may include a variety of naturally-occurring proteins and derivatives thereof, e.g., genetically engineered variants. For example, fluorescent proteins include green fluorescent protein (GFP), enhanced GFP, red, blue, yellow, cyan, and sapphire fluorescent proteins, reef coral fluorescent protein, etc. Luminescent proteins include luciferase and aequorin, and derivatives thereof. Numerous fluorescent and luminescent dyes and proteins are known in the art (see, e.g., U.S. Patent Application Publication No. 2004/0067503; Valeur, B., “Molecular Fluorescence: Principles and Applications,” John Wiley and Sons, 2002; and Handbook of Fluorescent Probes and Research Products, Molecular Probes, 9th edition, 2002).
Again, methods suitable for obtaining (e.g., synthesizing), stapling, and purifying the internally cross-linked polypeptides and modified polypeptides disclosed herein are also known in the art (see, e.g., Bird et. al., Methods in Enzymology 446:369-386 (2008); Bird et al, Current Protocols in Chemical Biology 2011; Walensky et al., Science 305:1466-1470 (2004); Schafmeister et al., J. Am. Chem. Soc. 122:5891-5892 (2000); U.S. Patent Application Publication No. 2010/0168388; and U.S. Pat. No. 7,723,468, each of which are hereby incorporated by reference in their entirety).
In some embodiments, the internally cross-linked polypeptides or modified polypeptides are substantially free of non-stapled peptide contaminants or are isolated. Methods for purifying internally cross-linked polypeptides or modified polypeptides include, for example, synthesizing the peptide on a solid-phase support. Following cyclization, the solid-phase support may be isolated and suspended in a solution of a solvent such as DMSO, DMSO/dichloromethane mixture, or DMSO/NMP mixture. The DMSO/dichloromethane or DMSO/NMP mixture may comprise about 30%, 40%, 50%, or 60% DMSO. In a specific embodiment, a 50%/50% DMSO/NMP solution is used. The solution may be incubated for a period of 1, 6, 12, or 24 hours, following which the resin may be washed, for example with dichloromethane or NMP. In one embodiment, the resin is washed with NMP. Shaking and bubbling an inert gas into the solution may be performed.
Properties of the internally cross-linked polypeptides and modified polypeptides of the invention can be assayed, for example, using the methods described below. For example, any of the cross-linked polypeptides and polypeptides described herein can be tested for their ability to prevent or decrease stress-induced cell death (e.g., using methods described herein or known in the art) or to increase or induce cell death (e.g., apoptosis) (e.g., using fluorescence-assisted cell sorting, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and/or immunofluorescence microscopy, or any other methods known in the art for detecting cellular apoptosis or cell death).
Assays to Determine α-Helicity: Internally cross-linked polypeptides or modified polypeptides are dissolved in an aqueous solution (e.g. 5 mM potassium phosphate solution at pH 7, or distilled H2O, to concentrations of 25-50 μM). Circular dichroism (CD) spectra are obtained on a spectropolarimeter (e.g., Jasco J-710, Aviv) using standard measurement parameters (e.g. temperature, 20° C.; wavelength, 190-260 nm; step resolution, 0.5 nm; speed, 20 nm/sec; accumulations, 10; response, 1 sec; bandwidth, 1 nm; path length, 0.1 cm). The α-helical content of each internally cross-linked polypeptide or modified polypeptide is calculated by dividing the mean residue ellipticity by the reported value for a model helical decapeptide (see, e.g., Yang et al., Methods Enzymol. 130:208 (1986)).
Assays to Determine Melting Temperature (Tm): Internally cross-linked polypeptides/modified polypeptides or the unmodified template peptides are dissolved in distilled H2O or other buffer or solvent (e.g., at a final concentration of 50 μM) and Tm is determined by measuring the change in ellipticity over a temperature range (e.g., 4 to 95° C.) on a spectropolarimeter (e.g., Jasco J-710, Aviv) using standard parameters (e.g., wavelength 222 nm; step resolution, 0.5 nm; speed, 20 nm/sec; accumulations, 10; response, 1 sec; bandwidth, 1 nm; temperature increase rate: 1° C./min; path length, 0.1 cm).
In Vitro Protease Resistance Assays: The amide bond of the peptide backbone is susceptible to hydrolysis by proteases, thereby rendering peptidic compounds vulnerable to rapid degradation in vivo. Peptide helix formation, however, typically buries and/or twists and/or shields the amide backbone, and therefore may prevent or substantially retard proteolytic cleavage. The internally cross-linked polypeptides or modified polypeptides of the present invention may be subjected to in vitro enzymatic proteolysis (e.g., trypsin, chymotrypsin, and pepsin) to assess for any change in degradation rate compared to a corresponding uncross-linked or alternatively stapled polypeptide. For example, the internally cross-linked or modified polypeptide and a corresponding uncross-linked polypeptide are incubated with trypsin agarose and the reactions quenched at various time points by centrifugation and subsequent HPLC injection to quantitate the residual substrate by ultraviolet absorption at 280 nm. Briefly, the internally cross-linked polypeptide or modified polypeptide and the control uncross-linked polypeptide or alternatively stapled polypeptide (5 mcg) are incubated with trypsin agarose (Pierce) (S/E ˜125) for 0, 10, 20, 90, and 180 minutes. Reactions are quenched by tabletop centrifugation at high speed; remaining substrate in the isolated supernatant is quantified by HPLC-based peak detection at 280 nm. The proteolytic reaction displays first order kinetics and the rate constant, k, is determined from a plot of ln[S] versus time.
Internally cross-linked polypeptides or modified polypeptides and/or a corresponding uncross-linked polypeptide can be each incubated with fresh mouse, rat, and/or human serum (e.g. 1-2 mL) at 37° C. for, e.g., 0, 1, 2, 4, 8, and 24 hours. Samples of differing internally cross-linked polypeptide or modified polypeptide concentration may be prepared by serial dilution with serum. To determine the level of intact internally cross-linked polypeptide or modified polypeptide, the following procedure may be used: The samples are extracted, for example, by transferring 100 μL of sera to 2 mL centrifuge tubes followed by the addition of 10 μL of 50% formic acid and 500 μL acetonitrile and centrifugation at 14,000 RPM for 10 min at 4±2° C. The supernatants are then transferred to fresh 2-mL tubes and evaporated on Turbovap under N2<10 psi, 37° C. The samples are reconstituted in 100 μL of 50:50 acetonitrile:water and submitted to LC-MS/MS analysis. Equivalent or similar procedures for testing ex vivo stability are known and may be used to determine stability of macrocycles in serum.
In Vivo Protease Resistance Assays: A key benefit of peptide stapling is the translation of in vitro protease resistance into markedly improved pharmacokinetics in vivo. Liquid chromatography/mass spectrometry-based analytical assays can be used to detect and quantitate the levels of internally cross-linked polypeptides or modified polypeptides in plasma. For pharmacokinetic analysis, internally cross-linked polypeptides or modified polypeptides are dissolved in sterile aqueous 5% dextrose (1 mg/mL) and administered to C57BL/6 mice (Jackson Laboratory) by bolus tail vein or intraperitoneal injection (e.g., 5, 10, 25, or 50 mg/kg). Blood is collected by retro-orbital puncture at 5, 30, 60, 120, and 240 minutes after dosing 5 animals at each time point. Plasma is harvested after centrifugation (2,500×g, 5 minutes, 4° C.) and stored at −70° C. until assayed. Peptide concentrations in plasma are determined by reversed-phase high performance liquid chromatography with electrospray ionization mass spectrometric detection (see, e.g., Aristoteli et al., Journal of Proteome Res. 6:571-581, 2007; Walden et al., Analytical and Bioanalytical Chemistry 378:883-897, 2004). Study samples are assayed together with a series of 7 calibration standards of internally cross-linked polypeptide or modified polypeptide in plasma at concentrations ranging from 1.0 to 50.0 μg/mL, drug-free plasma assayed with and without addition of an internal standard, and 3 quality control samples (e.g., 3.75, 15.0, and 45.0 μg/mL). Standard curves are constructed by plotting the analyte/internal standard chromatographic peak area ratio against the known internally cross-linked polypeptide or modified polypeptide concentration in each calibration standard. Linear least squares regression is performed with weighting in proportion to the reciprocal of the analyte concentration normalized to the number of calibration standards. Values of the slope and y-intercept of the best-fit line are used to calculate the internally cross-linked polypeptide or modified polypeptide in study samples. Plasma concentration-time curves are analyzed by standard noncompartmental methods using WinNonlin Professional 5.0 software (Pharsight Corp., Cary, N.C.), yielding pharmacokinetic parameters such as initial and terminal phase plasma half-life, peak plasma levels, total plasma clearance, and apparent volume of distribution.
In Vitro Binding Assays: To assess the binding and affinity of internally cross-linked polypeptides or modified polypeptides to proteins (e.g., IP3R1), a fluorescence polarization assay (FPA) can be used, for example. The FPA technique measures the molecular orientation and mobility using polarized light and fluorescent tracer. When excited with polarized light, fluorescent tracers (e.g., FITC) attached to molecules with high apparent molecular weights (e.g., FITC-labeled internally cross-linked polypeptides or modified polypeptides bound to a large protein) emit higher levels of polarized fluorescence due to their slower rates of rotation as compared to fluorescent tracers attached to smaller molecules (e.g., FITC-labeled internally cross-linked polypeptides or modified polypeptides that are free in solution).
In Vitro Displacement Assays to Characterize Antagonists of Peptide-Protein Interactions: To assess the binding and affinity of compounds that antagonize the interaction between an internally cross-linked polypeptide or modified polypeptide and a protein (e.g., IP3R1 protein), a fluorescence polarization assay (FPA) utilizing a fluoresceinated internally cross-linked polypeptide or modified polypeptide is used, for example. The FPA technique measures the molecular orientation and mobility using polarized light and fluorescent tracer. When excited with polarized light, fluorescent tracers (e.g., FITC) attached to molecules with high apparent molecular weights (e.g. FITC-labeled internally cross-linked polypeptides or modified polypeptides bound to a large protein (e.g., IP3R1 protein) emit higher levels of polarized fluorescence due to their slower rates of rotation as compared to fluorescent tracers attached to smaller molecules (e.g. FITC-labeled internally cross-linked polypeptides or modified polypeptides that are free in solution). A compound that antagonizes the interaction between the fluoresceinated internally cross-linked polypeptide or modified polypeptide and an acceptor protein will be detected in a competitive binding FPA experiment.
Binding Assays in Intact Cells: It is possible to measure binding of peptides or internally cross-linked polypeptides or modified polypeptides to proteins (e.g., a IP3R1 protein) on or in intact cells by immunoprecipitation experiments.
Cellular Penetrability Assays: To measure the cell penetrability of the internally cross-linked polypeptides or modified polypeptides, intact cells are incubated with fluoresceinated internally cross-linked polypeptides (10 μM) for 4 hours in serum-free media or in media supplemented with human serum at 37° C., washed twice with media and incubated with trypsin (0.25%) for 10 minutes at 37° C. The cells are washed again and resuspended in PBS. Cellular fluorescence is analyzed, for example, by using either a FACSCalibur flow cytometer or Cellomics KineticScan® HCS Reader.
Clinical Trials: To determine the suitability of the internally cross-linked polypeptides, modified polypeptides, compounds, or pharmaceutical compositions of the invention for treatment of humans, clinical trials can be performed. For example, patients having a cancer or suspected of having a cancer requiring chemotherapy are selected and separated into treatment and one or more control groups, wherein the treatment group is administered an internally cross-linked polypeptide, modified polypeptide, compound, or pharmaceutical composition of the invention, while the control groups receive a placebo or a known cytoprotective drug. The treatment safety and efficacy of the internally cross-linked polypeptides, modified polypeptides, compounds, or pharmaceutical compositions of the invention can thus be evaluated by performing comparisons of the patient groups with respect to factors, such as prevention of symptoms, time to resolution of symptoms, and/or time to a decrease in the number, severity, or frequency of one or more symptoms of the disease. In some embodiments, subject administered an internally cross-linked polypeptide, modified polypeptide, compound, or pharmaceutical composition of the invention can have a reduced number of symptoms of the disease as compared to a subject in a control group receiving a placebo.
One or more of the proteins, polypeptides, or internally cross-linked polypeptides or modified polypeptides disclosed herein (e.g., one or more bclw mimetics, or bclw protein or BH4 domain-containing fragment thereof, or a polypeptide having an amino acid sequence at least 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identical to the amino acid sequence (e.g., the IP3R1 or Bax-interacting alpha helical face of the BH4 domain of bclw) can be formulated for use as or in pharmaceutical compositions. Such compositions can be formulated or adapted for administration to a subject via any route, e.g., any route approved by the Food and Drug Administration (FDA). Exemplary methods are described in the FDA Data Standards Manual (available at www.fda.gov/Drugs/DevelopmentApprovalProcess/FormsSubmissionRequirements/ElectronicSubmissions/DataStandardsManualmonographs/default.htm). For example, compositions can be formulated or adapted for administration by inhalation (e.g., oral and/or nasal inhalation (e.g., via nebulizer or spray)), injection (e.g., intravenously, intra-arterial, subdermally, intraperitoneally, intramuscularly, and/or subcutaneously), and/or for oral administration, transmucosal administration, and/or topical administration (including topical creams or ointments, topical (e.g., nasal) sprays, and/or topical solutions).
In some instances, pharmaceutical compositions can include an effective amount of one or more internally cross-linked polypeptides or modified polypeptides (e.g., an internally cross-linked polypeptide having the BH4 domain of bclw). The terms “effective amount” and “effective to treat,” as used herein, refer to an amount or a concentration of one or more internally cross-linked polypeptides and/or modified polypeptides or a pharmaceutical composition described herein utilized for a period of time (including acute or chronic administration and periodic or continuous administration) that is effective within the context of its administration for causing an intended effect or physiological outcome (e.g., prevention or treatment of CIPN and/or hearing loss).
Pharmaceutical compositions of this invention can include one or more internally cross-linked polypeptides and/or modified polypeptides and any pharmaceutically acceptable carrier, adjuvant and/or vehicle. In some instances, pharmaceutical compositions can further include one or more additional therapeutic agents in amounts effective for achieving a modulation of disease or disease symptoms.
In some instances, some pharmaceutical compositions of the present disclosure can include one or more agents for the alleviation of symptoms of a disorder described herein (e.g., agents for the alleviation of symptoms of CIPN or hearing loss).
In some instances, the pharmaceutical compositions of the present disclosure can include metformin. In some instances, the pharmaceutical compositions of the present disclosure can include one or more calpain inhibitors (e.g., AK275, MDL28170 (calpain inhibitor III, CAS 88191-84-8), PD150606, SJA6017, ABT-705253, and SNJ-1945).
The term “pharmaceutically acceptable carrier” refers to a carrier that can be administered to a patient, together with one or more internally cross-linked polypeptides and/or modified polypeptides of this invention, and which does not destroy the pharmacological activity thereof and is nontoxic when administered in doses sufficient to deliver a therapeutic amount of the one or more internally cross-linked polypeptide and/or one or more modified polypeptide.
Pharmaceutically acceptable carriers and vehicles that can be used in the pharmaceutical compositions of this invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, self-emulsifying drug delivery systems (SEDDS), such as d-α-tocopherol polyethyleneglycol 1000 succinate, surfactants used in pharmaceutical dosage forms such as Tweens or other similar polymeric delivery matrices, serum proteins (e.g., human serum albumin), buffer substances (e.g., phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, and salts), and electrolytes (e.g., protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, polyethylene glycol, and wool fat). Cyclodextrins (e.g., α-, β-, and γ-cyclodextrin) can also be advantageously used to enhance delivery of the internally cross-linked polypeptides and/or modified polypeptides described herein.
The pharmaceutical compositions of this invention can contain any conventional non-toxic pharmaceutically-acceptable carriers, adjuvants, or vehicles. In some cases, the pH of the formulation can be adjusted with pharmaceutically acceptable acids, bases, or buffers to enhance the stability of the formulated internally cross-linked polypeptide(s) and/or modified polypeptide(s), or its delivery form. The term “parenteral” as used herein includes subcutaneous, intra-cutaneous, intra-venous, intra-muscular, intra-articular, intra-arterial, intra-synovial, intra-sternal, intra-thecal, intra-lesional, and intra-cranial injection or infusion techniques.
Pharmaceutical compositions can be in the form of a solution, powder for inhalation and/or nasal administration, or cream or spray for topical administration. Such compositions can be formulated according to techniques known in the art using suitable dispersing or wetting agents (such as, for example, Tween 80) and suspending agents.
Pharmaceutical compositions can include preservatives and additives. Preservatives can be included to limit or prevent microbial growth or contamination. Exemplary preservatives can include, but are not limited to, thimerosal, benzethonium chloride (Phemerol), phenol, and 2-phenoxyethanol. Exemplary additives can include human serum albumin, gelatin, and antibiotics.
In some instances, one or more of the internally cross-linked polypeptides and/or modified polypeptides disclosed herein can be conjugated, for example, to a carrier protein. Such conjugated compositions can be monovalent or multivalent. For example, conjugated compositions can include one internally cross-linked polypeptide or modified polypeptide disclosed herein conjugated to a carrier protein. Alternatively, conjugated compositions can include two or more internally cross-linked polypeptides or modified polypeptides disclosed herein conjugated to a carrier. In such instances, additional cytoprotective components or chemotherapeutic agents can also be coupled to the carrier protein.
As used herein, when two entities are “conjugated” to one another, they are linked by a direct or indirect covalent or non-covalent interaction. In certain aspects, the association is covalent. In other aspects, the association is non-covalent. Non-covalent interactions include hydrogen bonds, van der Waals interactions, hydrophobic interactions, magnetic interactions, electrostatic interactions, etc. An indirect covalent interaction is when two entities are covalently connected, optionally through a linker group.
Carrier proteins can include any protein that increases or enhances the cytoprotective activity of a pharmaceutical composition in a subject. Polymeric carriers can be a natural or a synthetic material containing one or more primary and/or secondary amino groups, azido groups, or carboxyl groups. Carriers can be water-soluble.
In some aspects, the present disclosure provides methods for using these pharmaceutical compositions for treating or preventing the CIPN or hearing loss.
Although effective amounts can depend, among other things, on the species of subject treated, the body weight of the subject, and the chosen treatment regimen, effective amounts can be readily determined by those in the art.
Chemotherapy is typically used in the context of cancer treatment and uses one or more anti-cancer drugs referred to as chemotherapeutic agents as part of a standardized treatment regimen. Chemotherapy may be given with a curative intent (which almost always involves combinations of drugs), or it may aim to prolong life or to reduce symptoms (palliative chemotherapy).
Non-limiting examples of chemotherapeutic agents include: alkylating agents (e.g., mechlorethamine, chlorambucil, cyclophosamide, ifosfamide, melphalan, streptozocin, carmustine, lomustine, oxaliplatin, busulfan, dacarbazine, temozolomide, thiotepa, and altretamine), antimetabolites (e.g., 5-fluorouracil, capecitabine, 6-mercaptopurine, folic acid analogs (e.g., methotrexate), gemcitabine, arabinosides (e.g., cytarabine), fludarabine, and premetrexed), anthracyclines (e.g., daunorubicin, doxorubicin, epirubicin, and idarubicin), topoisomerase inhibitors (e.g., topotecan, irinotecan, and epipodophyllotoxins (e.g., etoposide and teniposode)), microtubule-targeting agents and spindle poisons (e.g., taxanes (paclitaxel and docetaxel), vinblastine, vincristine, vindesine, vinorelbine, vinflunine, discodermolide, eleutherobin, sarcodictyin, epothilone, ixaberpilone, colchicine, combretastatin, 2-methoxyestradiol, noscapine, and estramustine), proteasome inhibitors, EGF-R inhibitors, Eph-R inhibitors, p38/JAK kinase inhibitors, PI3K inhibitors, MEK inhibitors, MAPK inhibitors, Trk inhibitors, proteasome inhibitors, Raf inhibitors, corticosteroids (e.g., dexamethasone, prednisolone, and methyl prednisolone), platinum compounds, and therapeutic antibodies (e.g., bevacizumab, brentuximab vedotin, cetuximab, ibritumomab tiuxetan, ipilimumab, panitumumab, rituximab, tositumomab, and trastuzumab). Chemotherapy often produces several side-effects.
Chemotherapy-induced peripheral neuropathy (CIPN), a type of chemotoxic axon injury, is a common and dose-limiting side effect of numerous cytotoxic chemotherapies in oncology. CIPN constitutes a frequent cause of axon injury and neurological impairment. Patients with CIPN experience pain, tingling, numbness, and/or impaired motor function, due to degeneration of long peripheral sensory or motor neuron axons.
Chemotherapeutic drugs that result in CIPN include microtubule-targeting agents. e.g., CIPN can result from use of chemotherapeutic agents such as, but not limited to, taxanes (e.g., paclitaxel, docetaxel), vinca alkaloids (e.g., vinblastine, vinorelbine, vindesine, vinflunine, vincristine) alkylating agents, arabinosides, proteasome inhibitors, PI3K inhibitors, Raf inhibitors, discodermolide, eleutherobin, sarcodictyin, epothilone, colchicine, combretastatin, 2-methoxyestradiol, and noscapine. Microtubule-targeting chemotherapeutic agents (e.g., those used to treat breast, ovarian, and lung cancers) cause a primarily sensory neuropathy. The mechanism for the chemotherapy-induced degeneration is not understood, and there are currently no treatments available for this common disorder.
This disclosure shows that microtubule targeting chemotherapeutic agents reduce axonal expression of bclw, but does not alter expression of the closely related components Bcl2 or BclxL. bclw was shown to protect axons from degeneration, and microtubule-targeting drugs such as paclitaxel cause degeneration by discontinuing this protection by lowering axonal levels of bclw. The BH4 domain of bclw is sufficient to reduce or prevent axonal degeneration. Accordingly, bclw protein or BH4 containing bclw polypeptides as well as bclw mimetics provide a clinically useful therapy for CIPN.
The disclosure includes methods of using an effective amount of one or more of the mimetics, polypeptides, or pharmaceutical compositions described herein (e.g., one or more bclw mimetics or polypeptides having an amino acid sequence at least 70% to 100% identical to the amino acid sequence of the BH4 domain of bclw) for the prevention or treatment of CIPN in a subject in need thereof. In some instances, the methods involve administering a bclw protein or a BH4 domain-containing fragment thereof. In some cases, the BH4 domain-containing bclw fragment is 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some instances, the methods involve administering a bclw polypeptide comprising or consisting of an amino acid sequence set forth in SEQ ID NOs.: 2 or 3. In some cases, the bclw polypeptide is 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some cases, the bclw polypeptide comprises or consists of an amino acid sequence set forth in SEQ ID NOs.: 2 or 3 with one to twelve amino acid substitutions (e.g., 1, 2, 3, 4, 5) and/or one to five amino acid deletions (e.g., 1, 2, 3 at the N or C-terminal of the polypeptide). In some cases, the bclw polypeptide is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some instances, the methods involve administering a bclw mimetic comprising or consisting of any one of the amino acid sequences set forth in SEQ ID NOs. 4 to 32 or 50-53. In certain instances, the methods involve administering a bclw mimetic comprising or consisting of any one of the amino acid sequences set forth in SEQ ID NOs. 4 to 32 or 50-53 except for 1, 2, 3, 4, or 5 amino acid substitutions and/or deletions (e.g., at N or C-terminus; IP3R non-interacting alpha helical face; Bax non-interacting alpha helical face). The method can further comprise administering other therapeutically beneficial agents to the subject. For example, metformin and/or one or more calpain inhibitors can be administered before, during, and/or after administration of the bclw mimetics, bclw polypeptides, or pharmaceutical compositions described herein.
The term “subject,” as used herein, refers to any mammal. In certain aspects, the term “subject,” as used herein, refers to a human (e.g., a man, a woman, or a child).
The terms “administer,” “administering,” or “administration,” as used herein refers to implanting, absorbing, ingesting, injecting, or inhaling, one or more of the inventive internally cross-linked polypeptides, modified polypeptides, or pharmaceutical compositions (e.g., any of those described herein). In some instances, one or more of the internally cross-linked polypeptides, modified polypeptides, compounds, or pharmaceutical compositions disclosed herein can be administered to a subject topically and/or orally. For example, the methods herein include administration of an effective amount of one or more internally cross-linked polypeptides, modified polypeptides, compounds, or pharmaceutical compositions to achieve the desired or stated effect. The internally cross-linked polypeptides, modified polypeptides, compounds, and pharmaceutical compositions of this invention can be administered at least once a week (e.g., about twice a week, about three times a week, about four times a week, about five times a week, about six times a week, or about 1 to about 6 times per day) or alternatively, as a continuous infusion. Such administration can be used as a chronic or acute therapy.
Specific dosage and treatment regimens for any particular patient will depend upon a variety of factors, including the activity of the specific internally cross-linked polypeptide, modified polypeptide, or compound employed, the age, body weight, general health status, sex, diet, time of administration, rate of excretion, drug combination, the severity and course of the CIPN, the patient's disposition to the treatment, and the judgment of the treating physician.
Following administration, the subject can be evaluated to detect, assess, or determine the number of symptoms and/or the severity and/or frequency of one or more symptoms of the CIPN in the subject. In some instances, treatment can continue until a reduction in the number of symptoms and/or the severity and/or frequency of one or more symptoms of CIPN is observed. Upon improvement of a patient's condition, a maintenance dose of a mimetic, polypeptide, or pharmaceutical composition, or a combination thereof, of the disclosure can be administered, if necessary. Subsequently, the dosage or frequency of administration, or both, can be reduced, as a function of the symptoms, to a level at which the improved condition is retained. Patients can, however, require intermittent treatment on a long-term basis upon any recurrence of CIPN.
Also provided are methods of administering chemotherapy to a subject in need thereof. The method involves administering the chemotherapy and an effective amount of one or more of the bclw mimetics, bclw polypeptides, or pharmaceutical compositions described herein to the subject. The chemotherapeutic agents and the mimetics, polypeptides, or pharmaceutical compositions can be administered simultaneously or sequentially (e.g., the chemotherapeutic agents can be administered prior to or after the bclw mimetics, bclw polypeptides, or pharmaceutical compositions). In certain instances the chemotherapeutic agent is selected from the group consisting of a microtubule-targeting agent, an alkylating agent, an antimetabolite, a folic acid analogue, a spindle poison, a platinum compound, an epipodophyllotoxin, an antibiotic, an EGF-R inhibitor, an Eph-R inhibitor, a p38/JAK kinase inhibitor, a PI3K inhibitor, a MEK inhibitor, a MAPK inhibitor, a Trk inhibitor, a proteasome inhibitor, and a Raf inhibitor. In some instances, the subject is administered an additional therapeutic agent. For example, metformin and/or one or more calpain inhibitors can be administered before, during, and/or after administration of the chemotherapeutic agents and/or the bclw mimetics, bclw polypeptides, or pharmaceutical compositions. In some cases, the subject being administered chemotherapy has a hematological tumor (e.g., acute myeloid leukemia, chronic myeloid leukemia, Hodgkin lymphoma, non-Hodgkin lymphoma, multiple myeloma, acute lymphoblastic leukemia, or chronic lymphocytic leukemia). In certain instances, the subject being administered chemotherapy has a breast cancer, ovarian cancer, or lung cancer. In some instances, the methods involve administering a bclw protein or a BH4 domain-containing fragment thereof. In some cases, the BH4 domain-containing bclw fragment is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some instances, the methods involve administering a bclw polypeptide comprising or consisting of an amino acid sequence set forth in SEQ ID NOs.: 2 or 3. In some cases, the bclw polypeptide is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some cases, the bclw polypeptide comprises or consists of an amino acid sequence set forth in SEQ ID NOs.: 2 or 3 with one to twelve amino acid substitutions (e.g., 1, 2, 3, 4, 5) and/or one to five amino acid deletions (e.g., 1, 2, 3 at the N or C-terminal of the polypeptide). In some cases, the bclw polypeptide is 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some instances, the methods involve administering a bclw mimetic comprising or consisting of any one of the amino acid sequences set forth in SEQ ID NOs. 4 to 32, or 50-53. In certain instances, the methods involve administering a bclw mimetic comprising or consisting of any one of the amino acid sequences set forth in SEQ ID NOs. 4 to 32 or 50-53, except for 1, 2, 3, 4, or 5 amino acid substitutions and/or deletions (e.g., at N or C-terminus).
Also provided are methods of selecting or identifying a subject at risk of developing CIPN. The selected subjects can then be treated using any of the above-described methods. The subjects can be identified or selected on the basis of one or more of the following criteria: having reduced levels of endogenous bclw in the axons of the sensory neurons relative to a control subject who does not develop CIPN upon administration of a chemotherapeutic agent (e.g., a taxane such as paclitaxel), reduced levels of EphA4 or Eph/5 receptors in sensory neurons relative to a control subject who does not develop CIPN upon administration of a chemotherapeutic agent (e.g., a taxane such as paclitaxel), reduced expression of transporters relative to a control subject who does not develop CIPN upon administration of a chemotherapeutic agent (e.g., a taxane such as paclitaxel), reduced expression of tubulin relative to a control subject who does not develop CIPN upon administration of a chemotherapeutic agent (e.g., a taxane such as paclitaxel), reduced expression of NGF, BDNF, and/or NT3 relative to a control subject who does not develop CIPN upon administration of a chemotherapeutic agent (e.g., a taxane such as paclitaxel); and/or genetic polymorphisms in or adjacent to genes such as EphA4/5 that may confer increased risk, or in the genes encoding calcium-interacting proteins such as calmodulin and parvalbumin. Subjects meeting one or more of these criteria are determined to have an enhanced risk of developing CIPN.
This disclosure also features methods of treating or preventing hearing loss in a subject in need thereof. The examples provided in this disclosure show that bclw is necessary for the maintenance of hearing during aging. Accordingly, administering bclw or a mimetic thereof is useful for treating or preventing hearing loss. The hearing loss may be, e.g., age-related, noise-induced, or chemotherapy-induced.
In some instances, the methods involve administering a bclw protein or a BH4 domain-containing fragment thereof. In some cases, the BH4 domain-containing bclw fragment is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some instances, the methods involve administering a bclw polypeptide comprising or consisting of an amino acid sequence set forth in SEQ ID NOs.: 2 or 3. In some cases, the bclw polypeptide is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some cases, the bclw polypeptide comprises or consists of an amino acid sequence set forth in SEQ ID NOs.: 2 or 3 with one to twelve amino acid substitutions (e.g., 1, 2, 3, 4, 5) and/or one to five amino acid deletions (e.g., 1, 2, 3 at the N or C-terminal of the polypeptide). In some cases, the bclw polypeptide is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In some instances, the methods involve administering a bclw mimetic comprising or consisting of any one of the amino acid sequences set forth in SEQ ID NOs. 4 to 32 or 50-53. In certain instances, the methods involve administering a bclw mimetic comprising or consisting of any one of the amino acid sequences set forth in SEQ ID NOs. 4 to 32 or 50-53, except for 1, 2, 3, 4, or 5 amino acid substitutions and/or deletions (e.g., at N or C-terminus). In certain instances, the protein, polypeptide, or mimetic is administered by injection (e.g., into the oval window).
Subjects for this treatment can be selected on the basis of having an advanced age (e.g., age greater than or equal to 65, 70, 75, 80, 85, 90, 95, or 100) and/or hearing loss (e.g., hearing thresholds greater than or equal to 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, or 85 db, as evaluated by standard clinical methods known in the art, e.g., pure tone audiometry for air conduction thresholds at 500, 1000, and 2000 Hz).
In general, these methods include selecting a subject and administering to the subject an effective amount of one or more of the mimetics, polypeptides, or pharmaceutical compositions described herein, and optionally repeating administration as required for the treatment of hearing loss.
The terms “administer,” “administering,” or “administration,” as used herein refers to implanting, absorbing, ingesting, injecting, or inhaling, one or more of the inventive internally cross-linked polypeptides, modified polypeptides, or pharmaceutical compositions (e.g., any of those described herein). In some instances, one or more of the internally cross-linked polypeptides, modified polypeptides, compounds, or pharmaceutical compositions disclosed herein can be administered to a subject by injection (e.g., into the oval window). In some instances, one or more of the internally cross-linked polypeptides, modified polypeptides, compounds, or pharmaceutical compositions disclosed herein can be administered to a subject by direct delivery via the tympanic membrane. In certain instances, the administration involves the use of a virus (e.g., adenovirus, adeno-associated virus, lentivirus vectors) or using ear drops. For example, the methods herein include administration of an effective amount of one or more internally cross-linked polypeptides, modified polypeptides, compounds, or pharmaceutical compositions to achieve the desired or stated effect. The internally cross-linked polypeptides, modified polypeptides, compounds, and pharmaceutical compositions of this invention can be administered at least once a week (e.g., about twice a week, about three times a week, about four times a week, about five times a week, about six times a week, or about 1 to about 6 times per day) or alternatively, as a continuous infusion. Such administration can be used as a chronic or acute therapy.
Specific dosage and treatment regimens for any particular patient will depend upon a variety of factors, including the activity of the specific internally cross-linked polypeptide, modified polypeptide, or compound employed, the age, body weight, general health status, sex, diet, time of administration, rate of excretion, drug combination, the severity of the hearing loss, the patient's disposition to the treatment, and the judgment of the treating physician.
Following administration, the subject can be evaluated to assess the hearing loss in the subject. In some instances, treatment can continue until hearing of the subject improves. Upon improvement of a patient's condition, a maintenance dose of a mimetic, polypeptide, or pharmaceutical composition, or a combination thereof, of the disclosure can be administered, if necessary. Subsequently, the dosage or frequency of administration, or both, can be reduced, as a function of the hearing capacity of the subject, to a level at which the improved condition is retained. Patients can, however, require intermittent treatment on a long-term basis upon any recurrence of hearing loss.
The following are examples of the practice of the invention. They are not to be construed as limiting the scope of the invention in any way.
All experimental procedures were done in accordance with the National Institutes of Health (NIH) guidelines and were approved by the Dana-Farber Cancer Institutional Animal Care and Use Committee (IACUC).
Animal use. Timed pregnant Sprague-Dawley rats were from Charles River. bclw−/− mice are described in Ross et al., Nat Genet., 18(3):251-6 (1998). Genotyping for the wild-type bclw gene and/or lacZ gene was performed by Transnetyx using the Bclw targeting sequence GCTCTGAACCTCCCCATGACTTAAATCCGTTGCTCTTTCT-TGGCCCTGCCCAGTGCCTCTGAGCATTTCACCTATCTCAGGAGC (SEQ ID NO:37) and the lacZ sequence CGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAGTCAGGCCACG-G (SEQ ID NO:38). Bclw mice were maintained on a C57Bl6EJ background.
Cell culture. Compartmented chamber (Campenot) cultures were prepared as described (see, e.g., Fenstermacher et al., Springer Protocols: Neuromethods, 103:105-124 (2015)), with modifications. Briefly, DRGs from embryonic day 15 (E15) rats of either sex were dissected and trypsinized. DRGs (1.2×105 cells) were plated in the center compartment of a Teflon divider (Camp10, Tyler Research; Campenot, 1982) affixed to a p35 culture dish coated with growth factor reduced Matrigel basement membrane (BD Biosciences) 1:45 in DMEM. Cultures were maintained in media consisting of Neurobasal (Invitrogen) with 2% B27 supplement (Invitrogen), 1% penicillin-streptomycin, 1% GlutaMAX (Life Technologies), 0.08% glucose, and 0.3 μM cytosine arabinoside (AraC) at 37° C., 7.5% CO2; BDNF+NGF (PeproTech) were added to the cell body compartment at a concentration of 10 ng/ml and to the axon compartment at a concentration of 100 ng/ml for 2d. On day 3, media was replaced and the AraC was omitted. On day 5, neurotrophins were removed from the cell body compartment and reduced to 1 ng/ml in the axon compartments for 3-5 days. For paclitaxel treatment experiments, 30 μM paclitaxel (Sigma-Aldrich) or vehicle control (0.1% DMSO) was added to either cell body or distal axon compartments on day 7 for 24 hours. For calpain inhibition experiments, 20 μM calpain inhibitor III (VWR) and paclitaxel were added simultaneously to distal axon compartment.
Microfluidic chambers cultures were prepared as described (see, e.g., Fenstermacher et al., Nature Neuroscience, 19:690-696 (2016)), with modifications. Briefly, 3×104E15 DRG neurons (4 μl volume) were plated into one channel of a microfluidic device (Xona Microfluidics, SND450) affixed to a PDL/laminin-coated cover glass (Fisherbrand Microscope Cover Glass; 24×40-1.5). Cells were plated in media with 0.3 μM AraC; 50 ng/mL NGF+BDNF was added to cell body wells, and 100 ng/ml was added to axon wells. On day 2, cell body neurotrophins were reduced to 10 ng/ml. On day 4, cell body neurotrophins were reduced to 1 ng/ml and axon neurotrophins were reduced to 10 ng/ml. For paclitaxel treatment experiments, 60 μM paclitaxel or 0.1% DMSO vehicle control was added on day 5 to axon wells for 48 hours. Cells were fixed at room temperature with 4% PFA diluted 1:2 in media for 10 min, then undiluted 4% PFA for an additional 10 min.
Mass cultures consisting of 3×105E15 DRG neurons were grown on Matrigel-coated p35 culture dishes in neurotrophin-enriched (100 ng/ml NGF+BDNF) media with 0.3 μg/ml AraC. On day 3, neurotrophins were reduced to 10 ng/ml) and cultures maintained for 3-6 more days.
Axonal degeneration assay. Compartmented chamber cultures were fixed at room temperature with 4% PFA diluted 1:2 in media for 10 min, then undiluted 4% PFA for an additional 20 min. Cultures were permeabilized with 0.1% TritonX-100 for 10 min, blocked in 3% BSA and 0.1% Triton X-100 for 1 hour at room temperature, and incubated with mouse anti-Tuj1 (1:400; clone Tuj1; Covance) overnight at 4° C. Cultures were then incubated with goat anti-mouse AlexaFluor (1:1000; Invitrogen) for 1 hour at room temperature and counterstained with DAPI. Images of distal axon tips were obtained using a 40× air objective, and axonal degeneration was quantified as a degeneration index, as previously described (Cosker et al., Neuroscience, 33:5195-5207 (2013)). Apoptosis analysis was carried out on the same cultures by taking images in the cell body compartment and counting total and condensed nuclei in NIH ImageJ software by an observer blind to condition.
Western blotting. For analysis of Bcl2 family proteins, cell bodies and axons of E15 DRGs in compartmented cultures were lysed in nonionic detergent, lysates were separated by 4-12% Bis-Tris or 3-8% Tris-Acetate SDS-Page (Thermo Fisher Scientific), and probed with the following antibodies: anti-Bclw (1:1000; clone 31H4; Cell Signaling Technology), anti-Bcl2 (1:1000; Abcam), anti-BclxL (1:1000; Cell Signaling Technology), and anti-GAPDH (1:2000; clone 14C10; Cell Signaling Technology). Bands were visualized with secondary antibodies conjugated to HRP (1:10,000; Bio-Rad) and SuperSignal chemiluminescent substrate signal. Using NIH ImageJ software, protein levels were quantified and levels of protein were normalized to GAPDH.
Calpain protease activity luminescence assay. DRG neurons in mass cultures were treated with vehicle (0.1% DMSO for 48h), paclitaxel (30 nM, 600 nM, or 1.2 μM for 48 hours), or calcium chloride (3 mM for 24 hours). Cultures were harvested in 1× Passive Lysis Buffer (Promega). The resulting lysate was spun 10,000×g for 5 min at 4° C., and 50 μL supernatant was combined with 50 μL Calpain-Glo Reagent (Promega) in a 96 well plate. The plate was shaken briefly and incubated for 40 min in the dark; luminescence intensity was measured with a microplate reader and normalized to protein concentration.
Tetramethylrhodamine ethyl ester measurements of mitochondrial membrane potential. E15 DRG neurons in microfluidic devices were labeled using Tetramethylrhodamine ethyl ester (TMRE) (Invitrogen). TMRE was applied (10 nM) for 20 min at 37° C., then cells were rinsed with phenol-free media and imaged live using a 60x oil 1.4NA objective. Fluorescence intensity of axonal mitochondria was measured by dividing the fluorescence intensity of each mitochondria (Fm) by the background fluorescence intensity of a nearby cytoplasmic region (Fc). Fluorescence intensity was measured using NIH ImageJ software for 80 mitochondria per condition across three experiments, by an observer blind to condition.
Quantitative reverse transcription-PCR. RNA was extracted from cultured neurons using TRIzol (Invitrogen) according to the manufacturer's protocol. Reverse transcription (RT) was performed using a cDNA archive kit (Applied Biosystems) according to the manufacturer's protocol. Quantitative real-time RT-PCR was performed using Taqman Gene expression assays (Applied Biosystems) to assess the expression of bclw (Rn00821025_g1), bcl2 (Rn99999125_m1), and bclxL (Rn00580568_g1). Data were normalized to gapdh (glyceraldehyde-3-phosphate dehydrogenase) for each sample (Applied Biosystems).
SAHB generation. FITC- or biotin-tagged stapled peptides corresponding to the BH4 domain of Bclw were synthesized, derivatized, and purified using our established methods (Bird et al., Nature, 455:1076-1081 (2008)). Stapled peptides were purified by LC-MS to >95% purity and quantified by amino acid analysis. Lyophilized SAHBs were reconstituted in 100% DMSO and diluted into aqueous buffers for experimentation.
Liposomal release assay. Large unilamellar vesicles with lipid composition resembling the mitochondrial outer membrane were generated and entrapped with ANTS and DPX as described (see, e.g., Leshchiner et al., Proc Natl Acad Sci USA, 110:E986-95 (2013); Lovell et al., Cell, 135:1074-1084 (2008)). Recombinant BAX was generated in B1-21 DE3 E. coli and then purified by affinity and size exclusion chromatography as described (see, e.g., Edwards et al., Chemistry & Biology, 20(7):888-902 (2013); Gavathiotis et al., Nature, 455:1076-1081 (2008)).
For measurement of BIMA2-induced activation of BAX, recombinant, full-length BAX protein, BIMA2 (amino acids 145-164), and BCLw BH4 SAHBs were added to liposomes (5 μl) at the indicated concentrations to a final volume of 30 μl in 384-well plate format. ANTS release and dequenching due to DPX dissociation (F) was measured over a period of 7200 sec with a Tecan M1000 plate reader (excitation and emission wavelengths of 355 and 520 nm, respectively). Plates were re-read following lysis with 1% Triton X-100 to determine maximal release (F100). Percent ANTS/DPX release was calculated as [(F−F0)/(F100−F0)].
Protein and peptide introduction. Recombinant His-tagged Bclw, Bcl2, and BclxL proteins (R&D Systems) or control β-galactosidase protein were introduced into cell bodies or axons of compartmented chamber cultures as described (e.g., Cosker et al., Nature Neuroscience, 19:690-696 (2016)). Briefly, 1 μg/μ1 protein was introduced into cultures using 2 μl Chariot reagent (Active Motif). To confirm expression of His-tagged proteins, cell bodies and axons were lysed in nonionic detergent, and protein lysates were separated as above and blotted with the following antibodies: anti-His (1:1000; Novagen) and anti-pan-actin (1:1000; Cell Signaling Technology). FITC-tagged SAHB peptides (BH4-Bclw, BH4-Bcl2, and BH4-BclxL; stock solutions 1 mM in DMSO) were introduced into cell bodies or axons using 350 ng peptide and 2 μl of Chariot reagent diluted 1:10 in water. Control was no peptide with 2 μl of 1:10 diluted Chariot. To confirm peptide expression, cultures were processed for axonal degeneration assay as above, and FITC immunofluorescence was examined with a 40× air objective. Paclitaxel was added to cultures 1 hour after protein or peptide transfection.
Neurotrophin deprivation. FITC SAHBs (FITC-BH4 Bclw, Bcl2, or BclxL) or no peptide control were transfected into the cell body compartment of compartmented cultures as described above. One hour later, both cell body and axon compartments were changed into media without NGF+BDNF for 24 hours. A non-transfected control culture was maintained in media with normal levels of NGF+BDNF. Cultures were fixed and incubated with DAPI (1:1000). Images were taken with a 40x air objective, and an observer blind to condition counted the number of total and condensed nuclei using NIH ImageJ software.
Biotinylated SAHB pulldowns. DRGs were grown in compartmented chamber cultures for 7-8 days, and cell bodies and axons were separately harvested in lysis buffer containing 1% CHAPS detergent, 150 mM NaCl, 50 mM Tris pH 7.4, 1 mM DTT, 500 mM NaF, 100 mM PMSF, 200 mM NaVO3, and EDTA-free cOmplete Mini protease inhibitor cocktail (Sigma-Aldrich). Lysate was pre-cleared 2 hours at 4° C. with High Capacity Neutravidin Agarose Beads (1:20 in lysate; Thermo Scientific). For each pulldown, 200-600 μg of precleared lysate was incubated overnight at 4° C. with biotin alone or with biotinylated-BH4 peptides of Bclw, Bcl2, or BclxL to a final peptide or biotin concentration of 20 μM. The next day, Neutravidin beads were added (1:14) to lysate for 2 hours at 4° C. Lysate was removed, beads were washed on ice with cold PBS+protease inhibitor cocktail, and sample was eluted by boiling for 5 minutes in non-ionic lysis buffer, sample buffer, and reducing agent. Protein was resolved with either 4-12% Bis-Tris SDS-page (Bax, YARS, IP3R1) or 3-8% Tris-Acetate SDS-page (YARS, IP3R1) and probed with the following antibodies at 1:1000: anti-Bax (Cell Signaling), anti-YARS (tyrosyl tRNA synthetase; clone EPR9927; Abcam), and anti-IP3R1 (Thermo Scientific). A 10% input lane from the original cell body or axon lysate was run alongside pulldown lysate. Band intensity was quantified as described above, and each pulldown intensity was normalized to the input intensity.
shRNA lentiviral knockdown. Lentiviral particles were generated using shRNA constructs from Sigma-Aldrich, and validated for protein knockdown in mass cultures. For degeneration assays, lentivirus expression shRNAs were added to compartmented cultures 1:1 in media for 24 hours, then media was changed and cultures were allowed to grow for 5 days prior to paclitaxel treatment. Bclw: TRCN0000321174, TRCN0000321104, TRCN0000321105, TRCN0000321106, TRCN0000004687 IP3R1: TRCN0000321161, TRCN0000273767, TRCN0000012439, TRCN0000012440, TRCN0000012442 IP3R3: TRCN0000434343, TRCN0000424063, TRCN0000416529, TRCN0000012444, TRCN0000012447.
Paclitaxel treatment and behavioral testing. 2 month old age-matched bclw−/− and bclw+/+ mice (17-30 g) of either sex were injected intraperitoneally (IP) with 4 mg/kg paclitaxel (Bristol-Myers Squibb) every other day for 8 days (4 total injections). Paclitaxel was prepared as 1 part 6 mg/ml paclitaxel stock solution diluted in vehicle (1:1 v/v Cremophor EL [EMD Millipore] and dehydrated ethanol) and 2 parts sterile saline and injected at 10 μl/g. Control mice were injected with 1 part vehicle and 2 parts saline. At 6 months of age, bclw−/− mice have altered noxious sensation; however, 2-3 month old bclw−/− mice exhibit normal motor function and noxious sensation (see, e.g., Courchesne et al., Neuroscience, 31:1624-1634 (2011)). For three days prior to the baseline testing, mice were weighed, trained on an automated RotaRod apparatus (4 rpm for 1 min without falling), and habituated in von Frey cages. The next two days, baseline behavioral performance was assessed and averaged. The first paclitaxel injection was given three days later, and mice were weighed and behaviorally tested 10 days after the final injection. To assay motor function, mice were placed on the RotaRod with a ramp of 4-40 rpm and 0.4 rpm/sec acceleration, and latency to fall was measured. Noxious mechanosensation threshold was assayed as described (see, e.g., Courchesne et al., 2011 (supra)), using von Frey filaments (0.008-1.4 g). Withdrawal threshold was determined to be the applied force at which the animal withdrew the stimulated paw on at least 2 of 10 applications. Noxious thermal sensation threshold was assayed as described (see, e.g., Courchesne et al., 2011 (supra)), using a 50° C. hot-plate and measuring latency to flick or lick the hindpaw. Mouse behavior was assessed by an experimenter blind to genotype and condition.
Epidermal footpad innervation. Footpad tissue from hindpaws of bclw−/− and bclw+/+ mice was harvested, fixed, and sectioned (see, e.g., Cosker et al., 2013). Briefly, mice were euthanized with isoflurane 11 days after final paclitaxel injection and footpad tissue was removed and divided into thick (dermal papillae containing) and thin (non-dermal papillae containing) skin. Footpads were fixed in Zamboni's fixative overnight at 4° C., cryopreserved in 30% sucrose overnight at 4° C., frozen, and sectioned into 30 μm floating sections. Sections were blocked in 10% normal goat serum with 0.1% Triton X-100 in PBS 1 hour at room temperature and incubated with anti-Tuj1 (1:300; Covance) overnight at 4° C. Sections were then incubated with goat anti-mouse AlexaFluor 488 (1:200; Invitrogen) and DAPI (1:1000) for 2 hours at room temperature and mounted on gelatin-coated slides. Epidermal images were acquired on a Nikon Ni-E C2 confocal with a 40× 1.3NA oil objective as 30-35 μm z-stacks (1 μm step size) and converted into a maximum intensity projection image. Intraepidermal nerve fiber density was determined to be the number of Tuj1-positive fibers penetrating into the epidermis, normalized to the measured epidermal length (225 μm-450 μm per image) and displayed as number of Tuj1-positive fibers per 225 μm. Images were acquired and quantified in NIH ImageJ by an experimenter blind to condition.
Statistics. Data are expressed as mean±SEM. To assess statistical significant, data were analyzed by unpaired two-tailed Student's t test. For multiple comparisons, data were analyzed by one-way ANOVA with post hoc Bonferroni or Dunnett correction. Significance was placed at p<0.05 unless otherwise indicated.
To determine the site of action of paclitaxel on sensory neurons, paclitaxel (30 nM) was introduced into the media surrounding either the cell body or distal axon compartments of E15 DRG sensory neurons in compartmented cultures, and paclitaxel-induced axonal fragmentation, a direct readout of degeneration, was analyzed. Paclitaxel added to axons was found to increase axon degeneration, while paclitaxel added to cell bodies has no effect (
Paclitaxel treatment reduced axonal mitochondrial membrane potential as assessed using the voltage-sensitive dye TMRE (
Although anti-apoptotic Bcl2 family members are involved in developmental axon degeneration, it is unclear if Bcl2 family members protect against axon degeneration caused by injury or disease. To identify anti-apoptotic Bcl2 family members that protect axons from the degenerative effects of paclitaxel, protein transfection was used in compartmented cultures to overexpress His-tagged recombinant Bclw, Bcl2, or BclxL protein selectively in axons. Transfected axonal Bclw completely prevented paclitaxel-induced axon degeneration (
BH4-Bclw inhibited BIM-induced, Bax-mediated membrane poration of ANTS/DPX encapsulated liposomes in a dose-dependent fashion (data not shown). FITC-tagged BH4 peptides of Bclw, Bcl2, or BclxL or a vehicle control were transfected into axons of compartmented cultures; the axons were then treated with paclitaxel for 24 hours (
The surprisingly specialized function of Bclw in preventing paclitaxel-induced degeneration can be because Bclw interacts with different molecular targets in axons than do Bcl2 and BclxL. As the BH4 domain of Bclw is alone sufficient to prevent paclitaxel-induced degeneration, co-precipitation of the biotinylated BH4 peptides of Bclw, Bcl2, or BclxL with IP3R1 was performed. In axon lysate, IP3R1 co-precipitated preferentially with BH4-Bclw (
To determine if Bclw regulation of IP3R1 is critical for preventing axon degeneration, neurons were infected with shRNAs targeting Bclw and/or IP3R1. Acute knockdown of Bclw increased spontaneous degeneration. Paclitaxel further increased axonal degeneration, suggesting that Bclw acts as a brake on the degenerative cascade activated by paclitaxel (
The expression of the anti-apoptotic components Bclw, Bcl2, or BclxL was examined after paclitaxel treatment. Strikingly, paclitaxel treatment reduced Bclw mRNA and protein levels selectively in axons and not in cell bodies (
To examine the role of Bclw in paclitaxel-induced degeneration in vivo, 2 month old bclw+/+ and bclw−/− mice were injected with paclitaxel (4 mg/kg every other day for 8 days). While bclw+/+ mice developed mild thermal hyperalgesia after paclitaxel treatment, as measured by a 50° hot-plate test, bclw−/− mice developed a more severe thermal pain sensitivity (
Bclw mRNA Expression Analysis in Cochlea.
RNA probe design and generation. RNA probes against Bclw were amplified from rat constructs using gene-specific sets of PCR primers which were designed by hand and purchased from Invitrogen (Table 1). To generate the Bclw probe, two rounds of PCR were performed before DIG labeling. Bclw forward and reverse primers were reacted with a rat Bclw construct, MgCl2 (Invitrogen), dNTPs (Roche), ddH2O, PCR Buffer (Invitrogen), and Taq DNA polymerase (Invitrogen). Each PCR included 30 cycles and an initializing step at 95 degrees Celsius for 2 min, a denaturation step at 95 degrees Celsius for 30 sec, an annealing step at 58 degrees Celsius for 30 sec, an elongation step at 72 degrees Celsius for 1 min, and a final elongation step at 72 degrees Celsius for 10 min. PCR products were run on a 1.2% agarose gel (loading buffer and 1.2% agarose) and bands with a length of ˜550 bp were extracted with a gel extraction kit (QIAGEN). Extracted DNA was subsequently used for a second PCR reaction, which used a Bclw forward primer and reverse nested primer containing the T7 promoter sequence (5′-TAA TAC GAC TCA CTA TAG GG-3′) (SEQ ID NO:45). For this second PCR, all steps were identical to the preceding PCR. PCR products were subsequently run on a 1.2% agarose gel and bands with a length of ˜500 bp were extracted with a gel extraction kit. To confirm the purity and sequence of the DNA, the PCR products were analyzed using a Nanodrop 8000 spectrophotometer (Thermo Scientific) and sequenced at the Dana-Farber DNA Sequencing Facility.
Bclw PCR products were DIG labeled to generate labeled riboprobes for in situ hybridization by reacting PCR products with 5x transcription buffer (Promega), 0.1 M DTT, T7 polymerase (Promega), RNasin (Promega), DIG labeling mix (Promega), and DEPC Water for 2 h at 37 degrees Celsius. 10 μl DNase (Promega) was added to the mixture and left to incubate for 10 min at 37 degrees. The reaction was stopped by adding 52 μl of stop buffer. DIG labeled RNA was extracted using an RNeasy Plus Mini Kit (QIAGEN) and resuspended in 50 μl of DEPC H2O before being diluted into hybridization buffer at 1 μg/ml (50% Formamide, 5×SSC, 0.2 mg/ml yeast tRNA, 100 μg/ml heparin, 1×Denhardt's solution, 0.1% Tween, 0.1% Chaps, 5 mM EDTA). RNA purity was confirmed using a Nanodrop 8000 spectrophotometer.
RNA probes against β-actin were amplified from rat constructs using gene-specific sets of β-actin forward, reverse, and reverse nested PCR primers which were designed by hand and purchased from Operon. The annealing temperature for the first and second PCR was 62 degrees Celsius. All other steps were consistent with the methods for generating Bclw RNA probes. β-actin RNA probes served as a positive control for in situ hybridization.
A scrambled sense probe was created by linearizing a miniprep Mef2A vector using vector-specific T7 and SP6 primers. An annealing temperature of 52 degrees Celsius was used during the linearizing step. All other methods were consistent with the methods for generating Bclw RNA probes. Scrambled probes served as a negative control for in situ hybridization.
Rats. Timed pregnant rats were purchased from Charles River. All rats dissected were wild-type littermates between ages P5 and P6. All procedures were performed in accordance with the NIH guidelines and were approved by the Dana-Farber Cancer IACUC.
Tissue preparation. Rats (P5 and P6) were sacrificed by isoflurane (Abbott) inhalation and immediately underwent intracardial perfusion with one body volume (approximately 15 mL) of DEPC-PBS followed by one body volume of 4% PFA in DEPC-PBS. Following perfusion, rats were decapitated and severed heads were either immediately dissected or stored overnight at 4° C. in a solution of 4% PFA in DEPC-PBS before dissecting the following day.
Rat heads were scored down the midline and cochlea were dissected in cold DEPC-PBS over ice. Cochlea were then drop-fixed in 4% PFA in DEPC-PBS overnight at 4 degrees Celsius.
After overnight fixation, tissues were then washed 3 times for 5 min in DEPC-0.1% PBST (PBS and 0.1% Triton X-100) over ice and then dehydrated in a graded methanol/0.1% PBST series (25%, 50%, 75%) into 100% methanol and stored at −20 degrees Celsius until needed.
In situ hybridization for BCLW mRNA expression analysis in cochlea. All in situ hybridization steps were completed using net wells in 12 well plates (BD Falcon) to reduce potential damage to the cochlea when transferred between washes. A total of 9 cochlea were used for each experiment since 3 cochlea were used to detect Bclw mRNA and another 6 cochlea were divided evenly for the positive ((3-actin) and negative (scrambled) controls. RNaseZap (Life Technologies) was used to decontaminate the lab bench and any tools used for in situ hybridization.
Day 1: Cochlea were removed from methanol and rehydrated stepwise into 100% DEPC-0.1% PBST. In each step, approximately 2 ml of solution was applied per well to cover all the tissue. Tissues were then washed 3 times for 5 min in DEPC-0.1% PBST and re-fixed in 4% PFA in DEPC-PBS for 20 min at room temperature. Cochlea were then washed 3 times for 5 min in DEPC-0.5% PBST at room temperature and pre-hybridized with hybridization buffer without probe for 1 hour at 65 degrees Celsius. Pre-hybridization buffer was replaced with hybridization buffer containing 1 μg/ml of probe and cochlea were incubated overnight at 65 degrees Celsius.
Day 2: Hybridization buffer was removed and cochlea were washed 2 times with pre-warmed Solution I (50% formamide, 5×SSC, 1% SDS, and DEPC-H2O) at 70 degrees Celsius for 30 min. Subsequently, tissue was washed once for 20 min at 70 degrees Celsius with 50% Solution I: 50% Solution II (0.5 M NaCl, 10 mM Tris HCl pH 7.5, 0.1% Tween-20, and DEPC-H2O), 3 times for 5 min with Solution II at room temperature, 2 times in Solution III (50% formamide and 2×SSC) for 30 min at 65 degrees Celsius, and 3 times for 5 min in TBST (50 mM Tris, 150 mM NaCl, 0.05% Tween 20). Tissues were then washed in blocking solution (TBST and 10% lamb serum) at room temperature for 2.5 h. Cochlea were then incubated on a rocker at 4 degrees Celsius overnight in blocking solution containing 1:2000 alkaline phosphatase-conjugated sheep anti-digoxigenin (Roche Diagnostics).
Day 3: Cochlea were removed from the blocking solution and washed 3 times for 5 min and then 4 times for 1 h in TBST. Tissues were left overnight in TBST at 4 degrees Celsius.
Day 4: Cochlea were removed from TBST and washed 2 times for 20 minutes in fresh alkaline phosphatase buffer (100 mM Tris pH 9.5, 50 mM MgCl2, 0.1% Tween-20, 100 mM NaCl, and DEPC-H2O) and then incubated in BM Purple (Roche) until signal was dissected under a dissecting microscope (Olympus SZ-ST). The reaction was stopped by washing 2 times for 5 min in 0.1% PBST/5 mM EDTA and fixing overnight in 4% PFA.
Day 5: After fixation, cochlea were transferred and washed 2 times for 10 min in 0.1% PBST/5 mM EDTA and imaged. After whole mount imaging, flat mounts were created and mounted on glass slides (Fisherbrand) using Fluoro-mount mounting media (Southern Biotec) and left in the dark at 4 degrees Celsius until further imaging.
Whole mount imaging. Whole mount imaging was done on a MVX10 MacroView dissecting microscope at the Harvard Medical School Neurobiology Imaging Facility.
Flat mount imaging. Flat mount imaging was done on a microscope at the 20× objective at Dana-Farber Cancer Institute.
Immunofluorescence for BCLW expression analysis in cochlea. Before experimentation, cochlea were removed from 4% PFA in DEPC-PBS and dissected to create flat mounts representative of the apical and basal turns. One cochlea was used to create the control flat mounts and once cochlea was used to create the experimental flat mounts for each experiment. All experiments were performed in 24 well plates, with one flat mount per well. The following protocol was used for all immunofluorescence experiments.
Day 1: Flat mounts were washed in PBS and incubated overnight at room temperature on a rocker. Approximately 500 μl of solution was used to cover the tissues.
Day 2: PBS was removed using a pipette and replaced with a permeabilizing solution (10% normal goat serum (NGS), 0.1% Triton X-100, and PBS) for 1 h at room temperature. After permeabilizing solution was removed, approximately 500 μl of primary antibody diluted in blocking solution (primary antibody, 5% NGS, and PBS) was applied to each well. Flat mounts were incubated in primary antibody solution overnight at 4 degrees Celsius on a rocker. The antibodies and dilutions used were as follows: BCLW rabbit (Cell Signaling) at 1:500 and TUJ1 mouse (Covance) at 1:300. Control flat mounts were not incubated in BCLW primary antibody and only received the TUJ1 primary antibody. Both control and experimental flat mounts were treated equally for all other steps in the immunofluorescence protocol.
Day 3: After overnight incubation, the primary antibody solution was removed and flat mounts were washed 4 times in PBS for one hour at room temperature to remove excess primary antibody. Approximately 500 μl of PBS was added to each well. Flat mounts were incubated overnight at 4 degrees Celsius in PBS.
Day 4: PBS was removed from wells and replaced with approximately 500 μl of secondary antibody (Alexa Fluor 488 goat anti-mouse (Invitrogen) at 1:400 or Alexa Fluor 546 goat anti-rabbit (Invitrogen) at 1:400) diluted in blocking solution (5% NGS and PBS). Each flat mount was incubated in the secondary antibody solution for 1 h at room temperature in the dark. After incubation in the secondary antibody solution, all subsequent steps were performed in the dark. After removal of the secondary antibody solution, flat mounts were washed 3 times in 500 μl of PBS for 5 min each. For nuclear staining, 500 μl of 4′,6-diamidino-2-phenylindole, or DAPI (5 mg/mL in ddH2O), was diluted 1:1000 in PBS and applied to washed flat mounts for 10 min. After removal of DAPI, flat mounts were washed 3 more times in PBS for 1 h each and incubated overnight on a rocker at 4 degrees Celsius to remove excess secondary antibody.
Day 5: Flat mounts were placed on superfrost glass slides (Fisherbrand) and received 50 μl of Fluoro-mount mounting media (Southern Biotec). Tissues were then cover slipped and allowed to dry overnight before imaging.
Imaging. Sections were imaged using a Nikon Eclipse TI inverted microscope with the 20× objective. Images were obtained using NIS Imaging software.
Quantification for BCLW expression analysis in cochlea. Images were quantified using ImageJ software to measure the pixel intensity of BCLW signal in cell bodies and axons. Signal intensity from cell bodies and axons was then normalized to controls. BCLW images for control and experimental tissues were then thresholded to remove background signal. After thresholding, both control and experimental BCLW images were brightened. All BCLW images from control and experimental tissues were manipulated equally to effectively capture the difference in signal.
Behavioral analysis of bclw+/+ and bclw−/− mice. The subjects were 31 bclw+/+ and bclw−/− mice on the C57Blk6EJ Blk6 background that does not contain the not mutation found in the Blk6J mice. 13 different litters contributed to both groups. The mice, originally obtained from Jackson Laboratory (Bar Harbor, Me.), were bred at the Dana-Farber Cancer Institute. The mice were maintained on a 12-hr light-dark cycle with lights on at 7 A.M. and were housed in groups of 1 to 5 mice. All mice were tested for hearing between 3 P.M. and 4 P.M. on testing days. Mice were labeled 1-31 in the order of their birth from oldest to youngest and genotypes were blinded until the conclusion of testing.
The number of mice used for the behavioral analysis was determined by power analysis using STATA and a two sample comparison of means. 6 months and 14 months were used as the mean ages of deafness onset for bclw−/− and bclw+1+ mice, respectively. Standard deviations of 8 months and 4 months were used for bclw−/− and bclw+1+ mice, respectively. The standard deviation for the mean age of deafness onset for bclw−/− mice was calculated by finding the difference between the means. P<0.05 was considered statistically significant. At most, 28 mice were needed for a 90% probability of capturing a statistically significant result.
In each training session, mice were tested for hearing in new, plastic cages provided by the Dana-Farber Cancer Institute Animal Facility. Cages were placed inside a hood with the light turned on but the blower turned off. Each behavior test was video recorded using an Apple iPhone 4S with 1080p HD video recording. All mice in the Dana-Farber Cancer Institute Animal Facility were habituated to white noise by playing background music continuously on a radio. This music persisted in the background during testing. An auditory stimulus, 86 dB, was produced by a Petco dog training clicker.
Mice were first tested at 6 weeks after birth and then every 3 weeks thereafter until 40 weeks of age. 40 weeks was selected as the endpoint for testing since mice on the Blk 6 background develop age-related hearing loss soon after that age, with complete degeneration of the organ of Corti observed by 12 months. During testing, each mouse was removed one at a time from its cage and placed in a new, fresh cage where it was allowed to habituate for 5 min. After the habituation time elapsed, the testing session began and the mouse was presented with three auditory stimuli, each separated by 3 to 6 sec. Responses to the auditory stimuli were video recorded from beginning to end of each testing session. After testing sessions, mice were returned to their original cages. All auditory tests were performed blind to the genotypes.
For each testing session, mice were labeled as having either strong, weak, or no hearing.
This designation was determined by observation of a Preyer's reflex, an elicitation of startle responses to auditory stimuli. A response was considered positive if there was rapid movement of the mouse's entire body or flicking of the pinna immediately following presentation of the auditory stimulus. Those that showed a Preyer's reflex following all three stimuli were labelled as having complete hearing ability. Mice responding to two stimuli were labelled as having weak hearing, and those responding to one or none of the stimuli were deemed deaf. Genotypes were blinded until the conclusion of experimentation. The time of deafness onset for each mouse was defined as the first test date in which the mouse no longer responded to any auditory stimuli. For these mice, all subsequent testing dates also showed no hearing ability. The mean ages of deafness onset for bclw+/+ and bclw−/− mice were compared using a Wilcoxon test (nonparametric t-test for means and proportions). P<0.05 was considered statistically significant.
Bclw mRNA is expressed in the cochlea along an apical-base gradient. It was hypothesized that Bclw plays a role in axonal maintenance of spiral ganglion neurons. This could explain the asymmetric degeneration of peripheral spiral ganglion axons observed during age-related hearing loss in humans, which parallels the retrograde degeneration of dorsal root ganglion peripheral axons in Bclw−/− mice. To evaluate this possibility, whole-mount in situ hybridization was performed using Bclw, β-actin, and scrambled RNA probes to detect the presence of Bclw mRNA in cochlea dissected from wild-type P5-P6 rats.
Because β-actin is known to be targeted to axons and locally translated to facilitate growth cone motility and axon guidance in response to neurotrophins, β-actin was expected to be present both in cell bodies and axons of spiral ganglion neurons. Given the hypothesis that Bclw is expressed in spiral ganglion neurons and transported to peripheral projections, it was predicted that the pattern of Bclw signal would be similar to the pattern of β-actin signal. In situ hybridization assays showed that β-actin and Bclw are both highly expressed within the cochlea (
To more precisely investigate the pattern of Bclw mRNA expression and localization in the cochlea, flat mounts of cochlear turns were created and imaged following whole mount in situ hybridization using Bclw, β-actin, and scrambled RNA probes. Neurogenesis and gene expression follow an apical-base gradient in which circuits develop first in the base of the cochlea, so it was important to determine whether Bclw mRNA expression differs between apical and basal turns. To test this possibility, images of flat mounts of basal and apical turns from the same cochlea were analyzed following in situ hybridization.
In situ hybridization showed robust β-actin mRNA expression in regions of the base and apex associated with spiral ganglion cell bodies. β-actin mRNA also appeared to localize to the spiral lamina where peripheral processes converge into radial bundles and extend to the sensory epithelium to innervate inner hair cells (IHCs) and outer hair cells (OHCs). This signal pattern was not diffuse in the spiral lamina but rather formed a band which persisted along the entire apical-base axis (
In parallel with molecular studies to assess the expression of Bclw in spiral ganglion neurons (Example 9), the function of Bclw in maintaining hearing during aging was investigated by behavioral analysis. To test the hypothesis that bclw−/− mice would have an earlier onset of hearing loss than wild-types, an experiment was designed whereby bclw−/− and wild-type mice on the same background (C57Blk6EJ) were measured for hearing ability at scheduled time periods during their lifespan. Based on studies of sensory neuron degeneration and associated deficits in thermosensation in bclw−/− mice, it was hypothesized that Bclw knockout mice would demonstrate hearing loss at 4 to 6 months of age. Though the Blk6 background is known to develop hearing loss after 12 months of age, this variable was controlled for by comparing knockouts on the C57Blk6EJ Blk6 background to wild-types that were also on the C57Blk6EJ Blk6 background. To assess hearing ability, mice were exposed to three auditory stimuli during each training session. Observation of a Preyer's reflex following a stimulus was considered a positive response. Based on the number of stimuli which received a positive response, mice were labeled as having either strong, weak, or no hearing ability. 31 mice were studied over the course of several months and the average age of deafness onset was compared between bclw−/− C57Blk6EJ Blk6 and wild-type C57Blk6EJ Blk6 mice.
Blinded results of the age of deafness onset showed a bimodal distribution of ages, indicating that mice are segregated into two populations which develop hearing loss either at early or later stages (
Four cohorts of animals were tested:
The noise exposure consisted of 100 dB of broadband noise for 2 hours. All animals were approximately 3 months old at the time and were treated and handled in parallel. Two weeks later, each mouse was tested for hearing, using two measures. First, we measured Distortion product otoacoustic emissions (DPOAE) responses, which are an indication of outer hair cell (and hence cochlear) function. Second, we measured Auditory Brainstem Responses (ABRs), which are created by the neural activity that passes from the cochlea to the brainstem. For DPOAEs and ABRs, we evaluated the threshold for response, i.e., how “loud” the sound needs to be to generate a signal either in the hair cells (DPOAE) or neurons (ABR). The hair cells activate the neurons, so if there is a change in hair cell activity, there will also be a change in neuronal activity. Thus, if DPOAE thresholds are unchanged, but ABR thresholds are changed, we can surmise that the neurons are primarily affected.
In addition, we measured the amplitude of the first ABR wave, which is a more sensitive measure of the neuronal response. When more neurons are activated synchronously, the amplitude increases. Thus, in wild-type animals, as the stimulus is made louder (i.e., increased sound pressure level, as measured in dB), the amplitude of the first wave (as measured in microvolts) also increases. If there is a loss of activated neurons, then the amplitudes are smaller and do not increase much with increased sound pressure levels.
The results from these tests are shown in
The salient findings are indicated below:
1. After noise exposure the CBA mice showed increased thresholds in the high frequency region of the cochlea (compare at 45 kHz).
2. In contrast, the mice that received bcl-w virus had preserved hearing at all frequencies.
3. Moreover, treatment with the virus did not affect baseline hearing (no threshold shift).
4. DPOAE thresholds were unchanged. This means that the noise exposure did not permanently damage the hair cells. This suggests that the preserved hearing in bcl-w treated animals reflects preservation of spiral ganglion neuron connections to inner hair cells in the high frequency region.
5. Consistent with this interpretation, CBA mice showed decreased wave 1 amplitudes in response to 45 kHz stimuli presented in 5 dB increments from 60 (the threshold) to 80 dB. In contrast, wave 1 amplitudes were unchanged in the bcl-w treated group.
Mice treated with paclitaxel exhibit decreased thick skin epidermal innervation compared with control mice. Loss of epidermal innervation is partially rescued when Bclw-BH4 SAHB peptide is injected into the footpad 24 hrs prior to paclitaxel treatment (paclitaxel-peptide) (
Human iPSC derived neurons were treated with paclitaxel. Pretreatment with Bclw BH4-SAHB (peptide 2) partially preserves axon length (
While the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.
This application is a divisional of U.S. patent application Ser. No. 16/326,493, filed on Feb. 19, 2019, which is a national stage of PCT/US2017/048582, filed Aug. 25, 2017, which claims the benefit of priority of U.S. Provisional Application No. 62/380,048, filed Aug. 26, 2016, the content of each of which is incorporated by reference herein in its entirety.
This invention was made with government support under grant numbers CA205255, AG000222, NS050674, and DC009223 awarded by The National Institutes of Health. The government has certain rights in the invention.
| Number | Date | Country | |
|---|---|---|---|
| 62380048 | Aug 2016 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 16326493 | Feb 2019 | US |
| Child | 17823018 | US |
