Claims
- 1. A method for producing a purified bone morphogenic protein-5 (BMP-5) said method comprising the steps of
- (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA sequence from nucleotide #699 to #2060 of Table III; and
- (b) isolating and purifying said protein from said culture medium.
- 2. An isolated DNA sequence encoding a bone morphogenic protein (BMP-5) comprising:
- (a) nucleotide #1647 through #2060 of Table III; and
- (b) naturally occurring allelic sequences and equivalent degenerative codon sequences thereof.
- 3. A vector comprising a DNA sequence of claim 2 in operative association with an expression control sequence therefor.
- 4. A host cell transformed with the vector of claim 3.
- 5. The host cell of claim 4 wherein said host cell is a mammalian cell.
- 6. The host cell of claim 4 wherein said host cell is a CHO cell.
- 7. A method for producing a bone morphogenic protein-5 (BMP-5) comprising:
- (a) transforming a host cell with a vector comprising nucleotide #1647 to #2060 of Table III;
- (b) culturing said transformed host cell in a suitable culture medium;
- (c) isolating and purifying said BMP-5 from said culture medium.
- 8. The method of claim 7 wherein said host cell is CHO.
- 9. A method for producing a bone morphogenic protein-5 (BMP-5) comprising:
- (a) transforming a host cell with a vector comprising nucleotide #1665 to #2060 of Table III;
- (b) culturing said transformed host cell in a suitable culture medium;
- (c) isolating and purifying said BMP-5 protein from said culture medium.
- 10. The method of claim 9 wherein said host cell is CHO.
- 11. A method for producing a bone morphogenic protein-5 (BMP-5) comprising:
- (a) transforming a host cell with a vector comprising nucleotide #1 to #2060 of Table III;
- (b) culturing said transformed host cell in a suitable culture medium;
- (c) isolating and purifying said BMP-5 from said culture medium.
- 12. The method of claim 11 wherein said host cell is CHO.
- 13. A composition comprising an isolated and purified (1) protein having the amino acid sequence from amino acid #317 (Ala) to amino acid #454 (His) as shown in Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
- 14. A composition comprising an isolated and purified (1) protein having the amino acid sequence from amino acid #323 (Asn) to amino acid #454 (His) as shown in Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
- 15. A composition comprising an isolated and purified (1) protein having the amino acid sequence from amino acid #1 (Met) to amino acid #454 (His) as shown in Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
- 16. A composition comprising (1) an isolated and purified protein dimer with at least one of the subunits having the amino acid sequence from amino acid #317 (Ala) to amino acid #454 (His) of Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
- 17. A composition comprising (1) an isolated and purified protein dimer with at least one of the subunits having the amino acid sequence from amino acid #323 (Asn) to amino acid #454 (His) of Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
- 18. A composition comprising (1) an isolated and purified protein dimer with at least one of the subunits having the amino acid sequence from amino acid #1 (Met) to amino acid #454 (His) of Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
- 19. A composition comprising (1) an isolated and purified protein dimer with at least one of the subunits having the amino acid sequence from amino acid #28 (Gly) to amino acid #454 (His) of Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
- 20. An isolated DNA sequence encoding a bone morphogenic protein-5 (BMP-5) comprising:
- (a) nucleotide #1665 through #2060 of Table III; or
- (b) naturally occurring allelic sequences or equivalent degenerative codon sequences thereof.
- 21. An isolated DNA sequence encoding a bone morphogenic protein-5 (BMP-5) comprising:
- (a) nucleotide #1 through #2060 of Table III; or
- (b) naturally occurring allelic sequences or equivalent degenerative codon sequences thereof.
- 22. An isolated DNA sequence encoding a bone morphogenic protein-5 (BMP-5) comprising:
- (a) nucleotide #699 through #2060 of Table III; or
- (b) naturally occurring allelic sequences or equivalent degenerative codon sequences thereof.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US90/01630 |
Mar 1990 |
WOX |
|
Parent Case Info
This application is a divisional of U.S. Ser. No. 08/116,425 filed Sep. 7, 1993, now U.S. Pat. No. 5,543,394 which is a continuation of U.S. Ser. No. 07/995,565 filed Dec. 22, 1992, now abandoned, which is a continuation of U.S. Ser. No. 07/588,227 filed Sep. 26, 1990, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/437,409 filed Nov. 15, 1989, now abandoned; U.S. Ser. No. 07/370,547 filed Jun. 23, 1989, now U.S. Pat. No. 5,106,748; U.S. Ser. No. 07/347,559 filed May 4, 1989, now abandoned, which is a continuation of U.S. Ser. No. 07/329,610 filed Mar. 28, 1989, now abandoned; which is a continuation-in-part of U.S. Ser. No. 07/179,100, now U.S. Pat. No. 5,014,649; U.S. Ser. No. 07/179,101, abandoned; and U.S. Ser. No. 07/179,197, abandoned, each filed Apr. 8, 1988, which are continuations-in-part of U.S. Ser. No. 07/028,285 filed Mar. 20, 1987, now abandoned and U.S. Ser. No. 07/031,346 filed Mar. 26, 1987, now U.S. Pat. No. 4,877,864 which are continuations-in-part of U.S. Ser. No. 06/943,332 filed Dec. 17, 1986, now abandoned and U.S. Ser. No. 06/880,776 filed Jul. 1, 1986, now abandoned.
US Referenced Citations (21)
Foreign Referenced Citations (5)
Number |
Date |
Country |
2 017466 |
May 1990 |
CAX |
33 6760 A2 |
Jun 1989 |
EPX |
4 165 78A2 |
May 1990 |
EPX |
4 094 72 A1 |
Nov 1990 |
EPX |
WO8909788 |
Oct 1989 |
WOX |
Related Publications (7)
|
Number |
Date |
Country |
|
370547 |
Jun 1989 |
|
|
347559 |
May 1989 |
|
|
179101 |
Apr 1988 |
|
|
179197 |
Apr 1988 |
|
|
179101 |
Apr 1988 |
|
|
179197 |
Apr 1988 |
|
|
31346 |
Mar 1987 |
|
Divisions (1)
|
Number |
Date |
Country |
Parent |
116425 |
Sep 1993 |
|
Continuations (3)
|
Number |
Date |
Country |
Parent |
995565 |
Dec 1992 |
|
Parent |
588227 |
Sep 1990 |
|
Parent |
329610 |
May 1989 |
|
Continuation in Parts (4)
|
Number |
Date |
Country |
Parent |
437409 |
Nov 1989 |
|
Parent |
179100 |
Apr 1988 |
|
Parent |
28285 |
Mar 1987 |
|
Parent |
943332 |
Dec 1986 |
|