Claims
- 1. A process for preparing a peptide having the amino acid sequence 95-126 of ANF/CDD 1-126 (gamma-hANaP), which is designated urodilatin (ANF/CDD 95-126), of the formula ##STR8## comprising step-wise synthesis on a solid phase using amino acids protected by protective groups.
- 2. The process according to claim 1, characterized in that the protective groups are tert-butyloxycarbonyl, fluorenylmethyloxycarbonyl and/or 3,5-dimethoxyphenyl-2,2-propyloxycarbonyl.
- 3. A process for obtaining from ANF/CDD 99-126 a peptide having the amino acid sequence 95-126 of ANF/CDD 1-126 (gamma-hANaP), which is designated urodilatin (ANF/CDD 95-126), of the formula ##STR9## comprising reacting ANF/CDD 99-126 with tetrapeptide Thr-Ala-Pro-Arg, having optional amino-protective groups, removing the optional amino-protective groups, and purifying the resulting product.
- 4. The process of claim 3 wherein, before reacting with the tetrapeptide, the ANF/CDD 99-126 is bonded to a solid phase, following reacting with the tetrapeptide, the solid phase is detached from the resulting peptide, and, following removing the optional amino-protective groups, subjecting the resulting product to cyclization and purification.
- 5. The process of claim 3 wherein, before reacting with the tetrapeptide, the ANF/CDD 99-126 is dissolved in a solvent, reacting with the tetrapeptide occurs in the presence of an activating agent, and, following reacting with the tetrapeptide and removing the optional amino-protective groups, the resulting product is subjected to chromatography and purified.
- 6. The process according to claim 3, characterized in that the tetrapeptide is included in Boc-Thr(But)-Ala-Pro-Arg(Tos).
- 7. A process for obtaining from urine a peptide having the amino acid sequence 95-126 of ANF/CDD 1-126 (gamma-hANaP), which is designated urodilatin (ANF/CDD 95-126), of the formula ##STR10## comprising adding alginic acid to the urine, eluting peptides adsorbed on the alginic acid, fractionating the eluate, and determining the urodilatin (ANF/CDD 95-126)-active fraction.
- 8. The process according to claim 7, characterized in that the eluate is salt-precipitated or lyophilized, the precipitate is desalted by gel chromatography, the obtained crude extract is subjected to ion exchange chromatography and subsequently separated by high performance liquid chromatography (HPLC).
- 9. The process according to claim 8, characterized in that the separation by HPLC is carried out using a reversed-phase silica gel.
- 10. The process of claim 7 wherein determining the urodilatin (ANF/CDD 95-126)-active fraction is by determining the fraction having a relaxing effect on smooth musculature.
Priority Claims (3)
Number |
Date |
Country |
Kind |
37 06 731.1 |
Mar 1987 |
DEX |
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37 17 329.4 |
May 1987 |
DEX |
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37 41 641.3 |
Dec 1987 |
DEX |
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Parent Case Info
This application is a divisional of Ser. No. 08/185,240, filed Jan. 24, 1994, U.S. Pat. No. 5,449,751, which is a continuation of Ser. No. 07/994,084, filed Dec. 16, 1992, abandoned, which is a continuation of Ser. No. 07/795,248, filed Nov. 18, 1991, abandoned, which is a continuation of Ser. No. 07/401,401, filed Sep. 1, 1989, abandoned, which is a continuation-in-part of PCT/EP88/00144, filed Feb. 27, 1988, abandoned.
US Referenced Citations (6)
Foreign Referenced Citations (1)
Number |
Date |
Country |
4870 |
Nov 1985 |
WOX |
Non-Patent Literature Citations (8)
Entry |
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Nakao et al, Horumon To Rinsho, vol. 33 (4): 365-370 (1985). |
Kangawa et al., Biochemical & Biophysical Research Communications, vol. 121(2):585-591 (1984). |
Seidah et al, Proc. Natl., Acad. Sci., vol. 81:2640-2644 (May 1984). |
Flynn et al, Biochemistry, vol. 232:313-321 (1985). |
Stewart et al, Solid Phase Peptide Synthesis, 2nd ed., published by Pierce Chemica Co. (1984). |
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Divisions (1)
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Number |
Date |
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Parent |
185240 |
Jan 1994 |
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Continuations (3)
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Number |
Date |
Country |
Parent |
994084 |
Dec 1992 |
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Parent |
795248 |
Nov 1991 |
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Parent |
401401 |
Sep 1989 |
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