Cd45 inhibitors

BACKGROUND

[0001] 1. Field of the Invention

[0002] Compounds, compositions and methods for the treatment of immunologically-related diseases and disorders such as autoimmune disorders and organ graft rejection.

[0003] 2. Related Art

[0004] Action of the immune system is known to be involved in immunologically-related diseases and disorders such as autoimmune disorders and in organ graft rejection (“OCR”). Hematopoietic, thymus-derived cells, (so-called “T cells”) have an important and pervasive role as regulators and effectors of the functions of the immune system. Hematopoietic cells, and T cells in particular have on their surfaces a major transmembrane glycoprotein designated CD45, characterized by a cluster of antigenic determinants. CD45 is also known as leukocyte common antigen (“LCA”). The cytosolic portion of CD45 has protein tyrosine phosphatase (“PTP”) activity and CD45 activity is known to be essential for TCR initiated T cell activation. Studies in CD45-deficient cell lines have shown that CD45 is a positive regulator of the T-Cell Receptor (“TCR”) and that CD45 functions in TCR regulation by dephosphorylating the src kinases p56lck and p59fyn). which allows autophosphorylation of the positive regulatory site on these enzymes; these reactions lead to downstream events and ultimately to T cell activation.

[0005] Available treatments for autoimmune disorders and OGR have therapeutic disadvantages. For example, Cyclosporin A, the drug most commonly used to treat OGR, has renal and CNS toxicity.

SUMMARY OF THE INVENTION

[0006] Potent inhibitors of CD45 have been discovered. Such inhibitors are useful for the treatment of various autoimmune disorders as well as for treatment of OGR. Inhibition of the phosphatase activity of CD45 by compounds of the present invention has been shown by incubating the cytosolic portion of CD45 with the compounds and p-nitrophenyl phosphate (pNPP), a phosphatase substrate. Spectrophotometric monitoring has shown that the liberation of p-nitrophenol from the substrate by CD45 is inhibited in the presence of the compounds disclosed herein. Inhibition of the phosphatase activity of CD45 by compounds of the present invention has also been shown using a p56lck carboxy-terminal phosphorylated peptide as a substrate. Compounds of the present invention have also been shown to inhibit proliferation of T cells in a T-cell proliferation assay.

[0007] Compounds of the present invention are substituted phenanthrene-9,10-diones in accord with structural diagram I:

[0008] wherein R1 at each occurrence is independently selected from hydrogen, halogen, NH2, NO2, NH—CO—R2, CO—NH—R2, Ar, (CH2)nCH(COOH)R3 COR3, NHCOCH2CH(COOH)NHR4 and N(R5)2;

[0009] in compounds in accord with structural diagram I, R2 is selected from (C1-C4)alkyl, (CH2)n,COOR6 and phenyl, wherein: n is an integer selected from the range 1 to 8; phenyl moieties of R2 can be substituted at one, two or three positions with a moiety selected from halogen, NO2 and CF3; alkyl moieties of R2 can be substituted with halogen, and R6 is selected from hydrogen and (C1-C4)alkyl;

[0010] in compounds in accord with structural diagram I where R1 is Ar, Ar at each occurrence is independently selected from groups in accord with the following structural diagrams:

[0011] or from phenyl which can be substituted with a moiety selected from halogen, (C1-C4)alkyl, O-(C1-C4)alkyl and halo(C1-C4)alkyl;

[0012] in compounds in accord with structural diagram I where R1 is COR3or (CH2)nCH(COOH)R3, R3 at each occurrence is an N-linked oligopeptide selected from GQ-N-iBu, GQPQP, QQPQP, EQPQP, GEPQP, QEPQP, GQGQP, QQGQP, EQGQP, GEGQP, QEGQP, QQPEG, GQGEP, GQPQG, QQPQG, GEPQG, GQPEG, GGPEG, EGPEG, RGPEG, GEPEG, EEPEG, REPI G, GRPEG, ERPEG, RRPEG, GGPRG, EGPRG, RGPRG, GEPRG, EEPRG, REPRG, GRPRG, ERPRG and RRPRG;

[0013] in compounds in accord with structural diagram I where R1 is NHCOCH2CH(COOH)NHR4, R4 at each occurrence is a C-linked N-acetyl-amino acid, -tripeptide or -oligopeptide selected from Q, EGQ, ATEGQ, TATEGQ, and FTATEGQ;

[0014] in compounds in accord with structural diagram I where R1 is N(R5)2, R5 at each occurrence is selected from hydrogen and tosyl.

[0015] Particular compounds of the present invention within the scope of compounds in accord with structural diagram I are substituted phenanthrene-9,10-diones in accord with structural diagram II:

[0016] wherein R5 is selected from hydrogen and tosyl.

[0017] Further particular compounds of the present invention within the scope of compounds in accord with structural diagram I are substituted phenanthrene-9,10-diones in accord with structural diagram III:

[0018] wherein R1 is Ar and Ar selected from groups in accord with the following structural diagrams:

[0019] or from phenyl which can be substituted with a moiety selected from halogen, (C1-C4)alkyl, halo(C1-C4)alkyl and O-(C1-C4)alkyl.

[0020] Yet further particular compounds of the present invention within the scope of compounds in accord with structural diagram I are substituted phenanthrene-9,10-diones in accord with structural diagram IV:

[0021] wherein R1 is independently selected at each occurrence from hydrogen, halogen, NO2, NH2, (CH2)nCH(COOH)R3 and COR3 where R3 is an N-linked peptide selected from GQ-N-iBu, GQPQP, QQPQP, EQPQP, GEPQP, QEPQP, GQGQP, QQGQP, EQGQP, GEGQP, QEGQP, QQPEG, GQGEP, GQPQG, QQPQG, GEPQG, GQPEG, GGPEG, EGPEG, RGPEG, GEPEG, EEPEG, REPEG, GRPEG, ERPEG, RRPEG, GGPRG, EGPRG, RGPRG, GEPRG, EEPRG, REPRG, GRPRG, ERPRG and RRPRG, with the proviso that no more than one R1 moiety is other than hydrogen.

[0022] Other particular compounds of the present invention within the scope of compounds in accord with structural diagram I are substituted phenanthrene-9,10-dionies in accord with structural diagram V:

[0023] wherein R1 is NHCOCH2CH(COOH)NHR4, where R4 is a C-linked N-acetyl-oligopeptide selected from Q, EGQ, ATEGQ, TATEGQ, and FTATEGQ.

[0024] Still further particular compounds of the present invention within the scope of compounds in accord with structural diagram I are phenanthrene-9,10-diones which are not substituted at positions 6 and 8 and wherein R1 is selected from hydrogen, NH2, NO2, and NHC(O)(CH2)nCOOCH3 where n is an integer selected from the range 1 to 8.

[0025] Compounds of the present invention are ligands of CD45 which, when bound, inhibit the activity of the protein tyrosine phosphatase (PTP) activity of the cytosolic portion of CD45. Binding of a compound of the present invention to CD45 inhibits the activity of CD45 essential for TCR initiated T cell activation. Thus, compounds of the invention inhibit the positive regulation of the TCR that leads to downstream events and T cell activation. Compounds of the present invention are useful to suppress the action of the immune system in immunologically-related diseases and disorders such as autoimmune disorders and organ graft rejection and to inhibit the action of T cells as functional regulators and effectors of the immune system.

[0026] The present invention also encompasses compositions made with compounds described herein useful for the treatment of immuniologically-related diseases and disorders and methods utilizing such compositions for treating such disorders.

DETAILED DESCRIPTION OF THE INVENTION

[0027] As described herein, the ring atoms of 9,10-phenanthrenediones are identified with a conventional numbering system, in accord with the following structural diagram:

[0028] As described herein, amino acids (“AA”) of oligopeptides are identified by conventional abbreviations or one-letter code, as follows: Glycine/Gly/G; Glutamic acid/Glu/E; Glutamine/Gln/Q; Proline/Pro/P; Leucine/Leu/L; Arginine/Arg/R; Phenylalanine/Phe/F; Tyrosine/Tyr/Y, and Threonine/Thr/T.

[0029] As used herein, (C1-C4)alkyl has its conventionally-understood meaning and particularly means linear or branched hydrocarbon chains having from one to four carbon atoms and thus includes methyl, ethyl, propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl, and the like.

[0030] As used herein, halo(C1-C4)alkyl has its conventionally-understood meaning and particularly means (C1-C4)alkyl as used herein wherein hydrogen atoms have been replaced by halogen atoms and thus includes monochloromethyl, trifluoromethyl, difluoroethyl, trifluoropropyl, perfluoro(C1-C4)alkyl, and the like.

[0031] As used herein perfluoro(C1-C4)alkyl has its conventionally-understood meaning and particularly means (C1-C4)alkyl as used herein wherein each hydrogen atom has been replaced by a fluorine atom and thus includes trifluoromethyl.

[0032] As used herein, (CH2)n has its conventionally-understood meaning and particularly means linear hydrocarbon chains having from one to n carbon atoms and thus includes methylene, ethylene, propylene, n-butylene groups, and the like.

[0033] As used herein, the terms halogen, halo, or halide have their conventionally-understood meanings and particularly mean chlorine, bromine, iodine or fluorine.

[0034] As used herein, the term tosyl has its conventionally-understood meaning and particularly means a group in accord with the following structural diagram:

[0035] As used herein, the term “from the range 1 to 6” or the like, means any integral value in the stated range, in this example 1, 2, 3, 4, 5 or 6.

[0036] Definitions of Terms:

[0037] DMF, N,N-dimethylformamide; THF, tetrahydrofuran; HATU, ( )-(7-Azabenzotriazol-1-yl)-N, N, N′,N′-tetramethyluroniumhexafluorophosphate; TLC, thin-layer chromatography; NMR, nuclear magnetic resonance; TFA, trifluoroacetic acid; FMOC, N-(9-fluorenylmethoxycarbonyl); HPLC, high performance liquid chromatography; HRMS, high resolution mass spectroscopy; EDC, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; DMAP, 4-dimethylaminopyridine; DMSO, dimethylsulfoxide; QTOF, quadropole time of flight; IC50, concentration giving 50% inhibition; CC50, concentration giving 50% cytotoxicity; AA, amino acid; ND, not determined.

[0038] HPLC method used: Analytical HPLC using an HP 1100 HPLC, with a C18 Dynamax column (5 cm×4.6 mm, 3 μM particle size, 100 Å pore size), flow rate of 0.5 mL/min, 20%-60% CH3CN in H2O over 7.5 min, holding at 60% CH3CN for 2.5 min, while monitoring at 254 and 210 nm.

[0039] HRMS method used: The sample was solubilized and diluted in MeOH. The QTOF mass spectrometer was calibrated using a PEG 400/600/1000 mixture solubilized in 50:50 acetonitrile/water with 50 mM ammonium acetate across a mass range 150-1150 Da. There was one sample submitted, with MW 1262 Da. This sample was run scanning the mass range 500-1300 Da using a calibration from the MW range 150-1300. The resolution for the instrument was measured at m/Δm=5100 at 50% peak height for the ion observed at m/z 953.1443. For each sample, 8 μL of solution was flow-injected into the mass spectrometer with a 35 μL/min flow of 80:20 acetonitrile/water (0.1% formic acid). A lock mass reference compound was infused into this flow at a rate of 2 μL/min (Ref. Compound exact mass 953.1443). Data was acquired from a single 3.5 minute analysis, and the signal was averaged from 0.9-1.8 min and then smoothed and centroided using a one-point lock mass correction using the reference compound listed above (m/z=953.1443).

EXAMPLES

Example 1

[0040] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-2-fluoro-benzamide:

[0041] To a solution of 2-amino-phenanthrene-9,10-dione (50 mg, 240 μmol) in THF (10 mL,) was added an excess of Na2CO3 (1g), followed by 2-fluorobenzoylchloride (46 μL, 384 μmol). The mixture was shaken overnight, then filtered and the solvent evaporated. The resulting material was purified on a silica gel column, using CH2Cl2-10% EtOAC/CH2Cl2 as the eluant to yield the pure amide N-(9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-2-fluoro-benzamide as a red solid. 1H NMR (300 MHz, DMSO-d6) δ10.80 (1H, s), 8.45 (1H, d, J=2.4 Hz), 8.30 (1H, d, J=9 Hz), 8.23 (1H, d, J=8 Hz), 8.09 (1H, dd, J=2.3, 8.9 Hz), 8.02(1H, dd, J=1.3, 7.7 Hz), 7.80-7.70 (2H, m), 7.62 (1H, m), 7.50 (1H, dd, J=7.3, 7.3 Hz), 7.38 (2H, m); HPLC: 6.97 min.

[0042] The compounds of examples 2 to 8 inclusive were prepared by the method of Example 1, by utilizing the appropriate acid chloride.

Example 2

[0043] 2-Benzyloxy-N-(9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-acetamide:

[0044] Mauve solid; 1H NMR (300 MHz, DMSO-d6) δ10.23 (1H, s), 8.38 (1H, d, J=2.1 Hz), 8.26 (1 H, d, J=9 Hz), 8.21 (1 H, d, J=7.8 Hz), 8.05-7.99 (2H, m),7.76 (1 H, dd, J=7.2, 7.2 Hz), 7.51-7.32 (6H, m), 4.65 (2H, s), 4.15 (2H, s); HPLC: 7.51 min.

Example 3

[0045] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-butyramide:

[0046] Red solid; 1H NMR (300 MHz, DMSO-d6) δ10.25 (1H, s), 8.30 (1H, d, J=2.4 Hz), 8.23 (1H, d, J=8.4Hz), 8.19 (1H, d, J=7.2Hz), 7.98 (2H, m), 7.75 (1H, ddd, J=7.9,7.9, 1.3 Hz), 7.47 (1H, dd, J=7.2 Hz), 2.34 (2H, t, J=7.2 Hz), 1.64 (2H, tq, J=7.4, 7.4 Hz), 0.93 (3H, t, J=7.3 Hz); HPLC: 5.86 min.

Example 4

[0047] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-2,2-dimethyl-propionamide:

[0048] Mauve solid; 1H NMR (300 MHz, CDCl3) δ8.41 (1H, dd, J=2.5, 8.7 Hz), 8.17 (1H, dd, J=1.3, 7.8 Hz), 7.98 (1 H, d, J=8.6 Hz), 7.94 (1H, d, J=7.2 Hz), 7.89 (1H, d, J=2.5 Hz), 7.70 (1H, ddd, J=1.4, 8,8 Hz), 7.54 (1H, br s), 7.44 (1H, dd, J=7.4, 7.4 Hz), 1.35 (9H, s); HPLC: 6.68 min.

Example 5

[0049] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-2-nitro-benzamide:

[0050] Purple solid; 1H NMR (300 MHz, DMSO-d6) δ11.04 (1H, s), 8.39 (1H, d, J=2.4 Hz), 8.31 (1H, d, J 9Hz), 8.24(1H, d, J=8.1 Hz), 8.19 (1H, d, J=8.1 Hz), 8.04-7.99(2H, m), 7.86-7.75 (4H, m), 7.50 (1H, dd, J=7.5, 7.5 Hz); HPLC: 6.39 min.

Example 6

[0051] 2,4,6-Trichloro-N-(9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-benzamide:

[0052] Red solid; 1H NMR(300 MHz, ˜1:3 DMSO-d6:CDCl3) δ10.89 (1H,s), 8.38 (1H, d, J=1.8 Hz), 8.26 (1H, dd, J=2.4, 8.7 Hz), 8.10 (1H,dd, J=1.1, 7.6 Hz),8.07 (1H, d, J=8.7 Hz), 8.05 (1H, d,J=7.8 Hz), 7.75 (1H, ddd, J=1.6, 7.9, 7.9 Hz), 7.47 (3H,m);HPLC:8.47 min.

Example 7

[0053] 2,2,2-Trichloro-N-(9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-acetamide:

[0054] Brown solid; 1H NMR (300 MHz, CDCl3) δ10.61 (1H, br s), 8.43 (1H, d, J=2.1 Hz), 8.35 (1H, dd, J=2.2, 9 Hz), 8.16 (1H, dd,J=1.2, 7.8 Hz), 8.07-8.01 (2H,m), 7.72 (1H, dd, J =7.5, 7.5 Hz), 7.47 (1H, dd, J=7.7, 7.7 Hz); HPLC: 7.65 min.

Example 8

[0055] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-2-trifluoromethyl-benzamide:

[0056] Brown solid; 1H NMR (300 MHz, ˜1:3 DMSO-d6:CDCl3) δ10.78 (1H, s), 8.43 (1H, d, J =2.2 Hz), 8.20 (1H, dd, J=2.5, 8.8 Hz). 8.10-8.06 (2H, m), 7.84-7.65 (6H, m), 7.46 (1H, dd, J=7.6, 7.6 Hz); HPLC: 7.37 min.

Example 9

[0057] N-(9,10-Dioxo-9,10-dihydro-phenenathren-3-yl)-butyramide:

[0058] To a solution of 3-amino-phenanthrene-9,10-dione (50 mg. 240 μmol) in THF (10 mL) an excess of Na2CO3 (0.5 g) was added, followed by butyrylchloride (1 mL). The mixture was shaken overnight, then water (1 mL) was added and the mixture was shaken for an additional 30 min. The mixture was filtered through a pad of silica gel using THF as the eluant, and the eluate evaporated under reduced pressure to yield a solid. The solid products were triturated with Et2O, and the residual solid material collected by vacuum filtration to yield the pure amide N-(9,10-dioxo-9,10-dihydro-phenanthren-3-yl)-butyramide as a brown solid. 1H NMR (300 MHz, DMSO-d6) δ10.44 (1H, s), 8.56 (1H, d, J=1.8 Hz),8.05-8.02 (3H,m), 7.84 (1H, ddd, J=1.5, 7.5, 7.5 Hz), 7.70 (1H, dd, J=1.8, 8.7 Hz), 7.56 (1H, dd, J=7.5, 7.5 Hz), 2.40 (2H, t, J=7.2 Hz), 1.66 (2H, tq, J=7.5, 7.5 Hz), 0.95 (3H, t, J=7.5 Hz); HPLC: 5.97 min.

[0059] The compounds of examples 10 to 13 inclusive were prepared by the method of Example 9, by utilizing the appropriate acid chloride.

Example 10

[0060] N-(9,10-Dioxo-9,10-dihydro-phenenathren-3-yl)-2,2-dimethyl-propionamide:

[0061] Brown solid; 1H NMR (300 MHz, DMSO-d6) δ9.68 (1H, s), 8.60 (1H, s), 8.06-8.02 (3H, m), 7.9 0(1H, dd, J=1.8, 8.7 Hz), 7.84 (1H, ddd, J=1.2, 8.1, 8.1 Hz), 7.76 (1H, dd, J=7.5, 7.5 Hz), 1.29 (9H, s); HPLC: 6.89 min.

Example 11

[0062] 2,2,2-Trichloro-N(9,10-dioxo-9,10-dihydro-phenanthren-3-yl)-acetamide:

[0063] Brown solid; 1H NMR (300 MHz, tfa shake-DMSO-d6) δ8.62 (1H, d, J=1.9 Hz), 8.31 (1H, s), 8.11 (2 H, m), 7.96 (1H, dd, J=1.8, 8.7 Hz), 7.86 (1H, ddd, J=1.5, 7.8, 7.8 Hz); HPLC: 7.76 min.

Example 12

[0064] N-(9,10-Dioxo-9,10-dihydro-phenanthren-3-yl)-2-fluoro-benzamide:

[0065] Yellow solid; 1H NMR (300 MHz, DMSO-d6) δ10.91 (1H, s), 8.64 (1H, s), 8.09-8.02 (3H, m), 7.89-7.82 (2H, m), 7.76 (1H, dd, J=7.5, 7.5 Hz), 7.68-7.57(2H, m),7.44-7.36(2H,m); HPLC: 7.15 min.

Example13

[0066] N-(9,10-Dioxo-9,10-dihydro-phenanthren-3-yl)-2-trifluoromethyl-benzamide:

[0067] Brown solid; 1H NMR (300 MHz, DMSO-d6) δ11.10 (1H, s), 8.61 (1H, s), 8.09-7.99 (3H, m), 7.91-7.75 (6H, m), 7.57 (1H, dd, J=7.5, 7.5 Hz); HPLC: 7.36 min.

Example 14

[0068] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)- 4-methyl-N-[(4-methylphenyl)sulfonyl]-benzenesulfonamide:

[0069] To a solution of 2-amino-phenanthrene-9,10-dione (200 mg, 900 μmol) in CH2Cl2 (10 mL) under N2, was added Et3N (630 μL, 4.48 mmol), p-toluenesulfonyl chloride (TsCl, 340 mg, 1.79 mmol) and a catalytic amount of N,N-dimethylaminopyridine. The resultant solution was stirred overnight, after which time it was diluted with ethyl acetate (25 mL) and washed sequentially with saturated aqueous NH4Cl, water and brine and then dried over Na2SO4. Filtration followed by evaporation under reduced pressure yielded a product which was purified by silica gel chromatography using CH2Cl2 as the eluant; the first material eluted from the column was the di-tosylate of 2-amino-phenanthrene-9,10-dione, N-(9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-4-methyl-N-[(4-methylphenyl)sulfonyl]-benzenesulfonamide (39 mg, 73 μmol, 8 %) which was dried to a yellow solid. 1H NMR (300 MHz, DMSO-d6) δ8.39(1H, d , J=8.7 Hz), 8.31 (1H, d, J=8.0Hz), 8.06(1H, dd, J=1.2, 7.7 Hz), 7.81 (1H, dd, J=7.5, 7.5 Hz), 7.73 (4H, d, J=8.3 Hz), 7.60 (1H, dd, J=7.5, 7.5 Hz), 7.54 (1H, m), 7.52 (4H, d, J=8.3 Hz), 7.36 (1H, dd, J=2.4, 8.5 Hz), 2.50 (6H, s);HPLC: 9.85 min.

Example 15

[0070] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-4-methyl-benzenesulfonamide:

[0071] A second material which eluted from the column was the mono-tosylate of 2-amino-9,10-phenenthrenedione, N-(9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-4-methyl-benzenesulfonamide (53 mg, 140 mmol, 16%) which was dried to an orange solid. 1H NMR (300 MHz, DMSO-d6) δ10.77 (1H, s), 8.17 (1H, d, J=9 Hz), 8.12 (1H, d, J=8.1 Hz), 7.96 (1H, dd, J=1.5, 7.8 Hz), 7.78-7.29 (4H, m), 7.48 (1H, s), 7.46 (1H, t, J=8.4 Hz), 7.38 (2H, d, J=8.1 Hz), 2.32 (3H, s); HPLC: 7.14 min.

Example 16

[0072] 2-Benzofuran-2-yl-phenanthrene-9,10-dione:

[0073] To a mixture of 2-bromo-phenanthrene-9,10-dione (150 mg, 520 μmol) in dioxane (5 mL) and H2 O (0.5 mL), benzo[b]furan-2-boronic acid (169 mg, 1.4 mmol), tris(dibenzylideneacetone)-dipalladium(0) (48 mg, 52 μmol), tri-o-tolylphosphine (32 mg, 1.4 mmol) and K2CO3 (220 mg, 1.57 mmol) were added. The resultant mixture was heated at 80° C. for 18 h and the solid material was removed by filtration. The soluble material was purified using a Rainin preparative HPLC on a Rainin phenyl column (25 cm×21.4 mm, 8 μM particle size, 60 Å, 40 mL/min, with 50% - 80% dioxane/H2 O for 40 min, followed by 80%-50% for 5 min. If the purity at this stage was not sufficient, the material was then chromatographed on a silica gel column using CH2-Cl2 as the eluant. Purple solid; 1H NMR (300 MHz, CDCl3) δ8.64 (1H, d, J =1.8 Hz), 8.21 (2H, ddd, J=1.2, 7.8, 7.8 Hz), 8.10 (1H, d, J=8.7 Hz), 8.05 (1H, d, J=7.8 Hz), 7.75 (1H, dd, J=7.8 ,7.8 Hz), 7.64 (1H, d, J=7.5 Hz), 7.57 (1H, d, J=8.1 Hz), 7.50 (1H, dd, J=7.8, 7.8Hz), 7.35 (1H, ddd, J=1.2, 6.6, 6.6 Hz), 7.31-7.28(2H, m); HPLC: 10.97 min.

[0074] The compounds of examples 17 to 22 inclusive were prepared by the method of Example 16, by utilizing the appropriate boronic acid.

Example 17

[0075]

2
-Thiophen-3-yl -phenanthrene-9,10-dione:

[0076] Orange solid; 1H NMR (300 MHz, CDCl3) δ8.40 (1H, d, J=2.1 Hz), 8.20 (1H, d, J=7.8 Hz), 8.04 (1H, d, J=8.1 Hz) 8.02 (1H, d, J=7.2 Hz), 7.94 (1H, dd, J=2.1, 8.4 Hz), 7.73 (1H, dd, J=7.5, 7.5 Hz), 7.64 (1H, m), 7.51-7.45 (3H, m); HPLC: 8.47 min.

Example 18

[0077] 2-(2-Fluoro-phenyl)-phenanthrene-9,10-dione:

[0078] Orange solid; 1H NMR (300 MHz, CDCl3) δ8.38 (1H, s), 8.23 (1H, d, J=7.8 Hz), 8.10 (1H, d, J=8.1 Hz), 8.07 (1H, d, J=8.1 Hz), 7.95 (1H,m), 7.75 (1H, dd, J=7.5,7.5 Hz), 7.56-7.51 (2H, m), 7.41-7.38 (1H, m), 7.28-7.20 (2H, m); HPLC: 8.76 min.

Example 19

[0079] 1 -Naphthalen-2-yl-phenanthrene-9,10-dione:

[0080] Orange solid; 1H NMR (300 MHz, CDCl3) δ8.35 (1H, d, J=2.1 Hz), 8.25 (1H, dd, J=1.2, 7.2 Hz), 8.16 (1H, d, J=8.4 Hz), 8.11 (1H, d, J=8.1 Hz), 7.96-7.84 (4H, m), 7.77 (1H, ddd, J=1.5, 7.7, 7.7 Hz), 7.52-7.47 (5H, m); HPLC: 10.31 min.

Example 20

[0081] 2-(2-Methoxy-phenyl)-phenanthrene-9,10-dione:

[0082] Orange solid; 1H NMR (300 MHz, CDCl3) δ8.36 (1H, d, J=1.9 Hz), 8.20 (1H, dd, J=1.4, 7.8 Hz), 8.06 (1H, s), 8.03 (1H, s), 7.93 (1H, dd, J=1.9, 8.3 Hz), 7.73 (1H, ddd, J=1.5, 7.6, 7.6 Hz), 7.47 (1H, ddd, J=0.6, 7.4, 7.4 Hz), 7.42-7.36 (2H, m), 7.10 (2H, m), 3.86 (3H, s); HPLC: 8.71 min.

Example 21

[0083]

2
-Benzo[1,3]dioxol-5-yl-phenanthrene-9,10-dione:

[0084] Violet solid; 1H NMR (300 MHz, CDCl3) δ8.33 (1H, d, J=2.1 Hz), 8.20 (1H, dd, J=1.5, 7.8 Hz), 8.04 (1H, d, J=8.1 Hz), 8.02 (1H, d, J=7.2 Hz), 7.86 (1H, dd, J=1.8, 8.4 Hz), 7.73 (1H, ddd, J=1.5, 7.8, 7.8 Hz), 7.47 (1H, ddd, J=0.9, 7.4, 7.4 Hz), 7.21-7.14(2H, m), 6.91 (1H, d, J=7.8 Hz), 6.10 (2H, s); HPLC: 8.71 min.

Example 22

[0085] 2-(2-Ethoxy-phenyl)-phenanthrene-9,10-dione:

[0086] Orange solid; 1H NMR (300 MHz, CDCl3) δ8.43 (1H, s), 8.21 (1H, d, J=7.8 Hz), 8.04 (2H, m), 7.96 (1H, d, J=8.4 Hz), 7.73 (1H, dd, J=7.2, 7.2 Hz), 7.49-7.34 (3H, m), 7.12-6.99 (2H, m), 4.09 (2H, q, J=7.2 Hz), 1.39 (3H, t, J=6.9 Hz); HPLC: 9.55 min.

Example 23

[0087] A Compound in Accord with Structural Diagram VII:

[0088] Polystyrene resin with the Ellman's aldehyde linker (50 mmol, see J. Org. Chem. 1997; 62, p. 1240) was swelled in DMF (1L) and acetic acid (10 mL) for 10 minutes, at which time isobutylamine (32 mL, 325 mmol) and sodium triacetoxyborohydride (69.5 g, 328 mmol) were added. The mixture was stirred with an overhead stirrer for two hours, then transferred to a fritted glass funnel and was washed with MeOH and DMF (1:1 mixture, 3×300 mL), DMF (3×300 mL), CH2Cl2 (5×300 ml) and methanol (5×300 ml). The resin was dried in vacuo at 40° C. for 16 h. The above resin (1.2 g, mmol) was swelled in DMF for 15 min, then washed three times with DMF. A solution of FMOCGln(Trt)OH (2.14 g, 3.5 mmol), HATU (1.14 g, 3 mmol), and diisopropylethylamine (1.1 mL, 6 mmol) in DMF (10 ml) was added to the resin and allowed to react for one hour. The liquid was drained and the resin was washed with DMF (10×10 mL). The resin was treated with 20% piperidine in DMF (10 mL) for 3 min, drained, then again treated with 20% piperidine in DMF (10 mL) for 7 min and drained. The resin was washed with DMF (10×10 mL). A mixture of FMOCGlyOH (1.04 g, 3.5 mmol). HATU (1.14 g, 3 mmol), and diisopropylethylamine (1.1 mL, 6 mmol) in DMF (10 mL) was added to the resin and allowed to react for 1 h. The liquid was drained and the resin was washed with DMF (10×10 mL). The resin was treated with 20% piperidine in DMF (10 mL) for 3 min, drained, then treated with another 20% piperidine in DMF (10 mL) for 7 min and drained. The resin was washed with DMF (10×10 mL). A mixture of 9,10-dioxo-9.10-dihydro-phenanthrene-3-carboxylic acid (880 mg, 3.5 mmol), HATU (1.14 g, 3 mmol), and diisopropylethylamine (1.1 mL, 6 mmol) was added to the resin and allowed to react for 1 h. The liquid was drained and the resin washed with DMF (10×10 mL), CH2Cl2 (5×10 mL), and Et2O (5×10 mL). The resin was dried for 16 h in vacuo, then treated with TFA containing 2% water and 2% thioanisole (10 mL) for 2 h. The solids were removed by filtration and washed with CH2Cl2 (10 mL). The volume of combined filtrate was reduced to about 1 mL by rotary evaporation and precipitation was induced by addition of Et2O (25 mL). The resulting solid was collected by vacuum filtration, washed with H2 O (3×25 mL) and dried in vacuo for 2 h. This product was purified by preparative HPLC on a C18 Dynamax column (21.4 mm×25 cm, 60 Å) using a gradient of 10% to 40% acetonitrile in water with 0.1% TFA. Fractions containing pure product were combined and lyophilized to give the compound in accord with structural diagram VII (170 mg) as an orange solid. Orange solid; HPLC: 3.95 min. Anal. Calcd. for C26H28N4O6-0.5H2O-0.5CF3CO2H C, 58.06;H, 5.32; N, 10.03. Found: C, 57.98, 58.31; H, 5.48, 5.40; N, 9.88, 9.88.

Example 24

[0089] A Compound in Accord with Structural Diagram VIII:

[0090] 6-Chlorochlortrityl resin (6.19 g, 7 mmol) was swelled in dry CH2Cl2 (30 mL) and a solution of FMOCprolineOH (1.18 g, 3.5 mmol) and diisopropylethylamine (1.8 mL, 10.5 mmol) in dry CH2Cl2 (10 mL) was added. The reaction was gently mixed under a nitrogen atmosphere for 30 min, then MeOH (3 mL) was added to cap the unreacted sites. After 15 min, the resin was transferred to a fritted glass funnel and washed with DMF (2×50 mL), CH2Cl2 (2×50 mL). MeOH (2×50 mL), and Et2O (2×50 mL). The resin was dried in vacuo at 50° C. to constant weight. A portion of this material (1.5 g, 0.25 mmol) was swelled in DMF (5 mL) for 15 min then washed with DMF (3×5 mL). The resin was treated with 20% piperidine in DMF (5 mL) for 3 min, drained, then treated with another 20% piperidine in DMF (5 mL) for 7 min and drained. The resin was washed with DMF (10×5 mL). A solution of FMOCglutamine(Trt)OH (610 mg, 1 mmol), HATU (380 mg, 1 mmol), and diisopropylethylamine (350 μL) in DMF (5 mL) was added to the resin and mixed with a gentle nitrogen bubbling for 2 h. The solution was drained and the resin was washed with DMF (10×5 mL). The resin was treated with 20% piperidine in DMF (5 mL) for 3 min, drained, then another portion of 20% piperidine in DMF (5 mL) was added and mixed for 7 min then drained. The resin was washed with DMF (10×5 mL). A solution of FMOCprolineOH (340 mg, 1 mmol), HATU (380 mg, 1 mmol), and diisopropylethylamine (350 μL) in DMF (5 mL) was added to the resin and mixed with a gentle nitrogen bubbling for 16 h. The solution was drained and the resin was washed with DMF (10×5 mL). The resin was treated with 20% piperidine in DMF (5 mL) for 3 min, drained, then another portion of 20% piperidine in DMF (5 mL) for 7 min and drained. The resin was washed with DMF (10×5 mL). A solution of FMOCglutamineOH (370 mg, 1 mmol), HATU (380 mg, 1 mmol), and diisopropylethylamine (350 μL) in DMF (5 mL) was added to the resin and mixed with a gentle nitrogen bubbling for 2.5 h. The solution was drained and the resin washed with DMF (10×5 mL). The resin was treated with 20% piperidine in DMF (5 mL) for 3 min, drained, then with another 20% piperidine in DMF (5 mL) for 7 min and drained. The resin was washed with DMF (10×5 mL). A solution of FMOCglutamineOH(OtBu) (830 mg, 1 mmol), HATU (380 mg, 1 mmol), and diisopropylethylamine (350 μL) in DMF (5 mL,) was added to the resin and mixed with a gentle nitrogen bubbling for 2 h. The solution was drained and the resin was washed with DMF (10×5 mL). The resin was treated with 20% piperidine in DMF (5 mL) for 3 min, drained, then with another 20% piperidine in DMF (5 mL) for 7 min and drained. The resin was washed with DMF (10×5 mL). A solution of 9,10-dioxo-9,10-dihydro-phenanthrene-3-carboxylic acid (250 mg, 1 mmol), HATU (380 mg, 1 mmol), and diisopropylethylamine (350 μL) in DMF (5 mL) was added to the resin and mixed with a gentle nitrogen bubbling for 16 h. The solution was drained and the resin was washed with DMF (10×5 mL), CH2Cl2 (5×5 mL), Et2O (5×5mL), then dried under a stream of N2. The resin was treated with TFA containing 2% thioanisole and 2% water (5 mL) for 3 h. The solids were filtered off and the filtrate was evaporated to a red oil. Addition of Et2O (10 mL) induced precipitation, and this solid was collected by vacuum filtration to give the product (300 mg) as a pink solid. The material was purified by preparative HPLC on a C18 Dynamax column (21.4 mm×25 cm, 60 Å) using a gradient of 10% to 26% acetonitrile in water with 0.1% TFA. Fractions containing pure product were combined and lyophilized to give M374187 (100 mg) as a yellow solid. HPLC: 2.55 min; Anal. Calcd. for C40H45N7O13-1.0H2O-0.5CF3CO2H C, 54.30; H, 5.28; N, 10.81; Found C, 54.10, 54.04; H, 5.19, 5.23;N, 10.99, 11.01.

[0091] The compounds shown in tables 1 and 2, below, were synthesized by the method of Example 24, using either 9,10-dioxo-9,10-dihydro-phenanthrene-3-carboxylic acid or 9,10-dioxo-9,10-dihydro-phenanthrene-2-carboxylic acid as the starting material and a suitable peptide fragment:

[0092] Table 1 shows compounds of the invention in accord with structural diagram VIII, having 3-carboxy-linked peptides.

1TABLE 1Example No.AA1AA2AA3AA4AA524GluGlnProGlnPro25GlyGlnProGlnPro26GlnGlnProGlnPro27GlyGluProGlnPro28GlnGluProGlnPro29GlyGlnGlyGlnPro30GlnGlnGlyGlnPro31GluGlnGlyGlnPro32GlyGluGlyGlnPro33GlnGluGlyGlnPro34GlnGlnProGluGly35GlyGlnGlyGluPro36GlyGlnProGlnGly37GlnGlnProGlnGly38GlyGluProGlnGly39GlyGlnProGluGly

Example 25

[0093] Yellow solid; HPLC: 2.67 min; Anal. Calc. For C37H41N7O11-1.5H2O-0.4CF3CO2H C,54.54; H, 5.38N, 11.78. Found: C, 54.52; 54.42; H, 5.40; 5.32;N, 11.72; 11.80.

EXAMPLE 26

[0094] Yellow solid; HPLC: 2.41 min.

EXAMPLE 27

[0095] Yellow solid; HPLC: 2.88 min; Anal. Calcd. for C37H40N6O12-1.0OH2O-0.5CF3CO2H C, 54.61; H, 5.13 N, 10.05. Found C, 54.82, 54.81; H, 5.51, 5.52; N, 10.01; 10.01.

EXAMPLE 28

[0096] Yellow solid; HPLC: 2.60 min; Anal. Calcd. for C40H45N7O13-1.5H2O-0.6CF3CO2H C, 53.37; H, 5.28; N, 10.57. Found C, 53.49; 53.43; H, 5.14, 5.15; N,10.85, 10.82.

Example 29

[0097] Yellow solid; HPLC: 2.34 min; Anal. Calcd. for C34H37N7O11-0.7H2O-0.6CF3CO2H C, 52.80; H, 4.91; N, 12.24. Found C, 52.59, 52.81; H, 4.92, 4.90; N, 12.53, 12.57.

Example 30

[0098] Yellow solid; HPLC: 2.07 min; Anal. Calcd. for C37H42N8O12-2.0H2O-0.6CF3CO2H C, 51.25; H, 5.25; N, 12.52. Found C, 51.31, 51.39; H, 4.97, 4.97; N, 12.75, 12.79.

Example 31

[0099] Yellow solid; HPLC: 2.47 min: Anal. Calcd. for C37H41N7O13-1.0H2O-0.5CF3CO2H C, 52.66; H, 5.06; N. 11.31. Found C, 52.79, 52.78; H, 5.17, 5.20; N, 11.31, 11.31.

Example 32

[0100] Yellow solid; HPLC; 2.52 min; Anal. Calcd. for C34H36N6O12-0.5H2O-0.5CF3CO21H C. 53.44; H, 4.80; N, 10.69. Found 53.29, 53.24; H, 4.96, 4.98; N, 10.73, 10.74.

Example 33

[0101] Yellow solid; HPLC: 2.25 min; Anal. Calcd. for C37H41N7O13-1.0H2O-0.5CF3CO2H C, 52.66; H, 5.06; N, 11.31. Found C, 52.59, 52.61; H, 5.17, 5.18; N, 11.46, 11.37.

Example 34

[0102] Yellow solid; HPLC: 2.13min; Anal. Calcd. for C37H41N7O13-1.5H2O-0.5CF3CO2H C, 52.11; N, 5.12; N, 11.20. Found C, 51.94, 51.98; H, 4.93, 4.93; N. 11.42, 11.42.

Example 35

[0103] Yellow solid: HPLC: 2.49 min; Anal. Calcd. for C34H36N6O12-1.5H2O-0.5CF3CO2H C, 52.24; H. 4.95: N, 10.44. Found C, 51.87, 51.91; H, 4.93, 4.86; N, 10.56, 10.57.

Example 36

[0104] Yellow solid. HPLC; 2.26min; Anal. Calcd. for C34H37N7O11-1.0H2O-0.5CF3CO2H C, 52.92, 53.08; H, 5.01, 5.00; N, 12.60, 12.66.

Example 37

[0105] Yellow solid; HPLC: 2.02 min; Anal. Calcd. for C37H42N8O12-1.0H2O-0.6CF3CO2H C, 52.30; H, 5.12; N, 12.77. Found C, 52.35, 52.28; H, 5.21, 5.15; N, 13.08, 13.05.

EXAMPLE38

[0106] Yellow solid; HPLC: 2.50 min; Anal. Calcd. for C34H36N6O12-0.5H2O-0.5CF3CO2H C, 53.4; H, 4.80; N, 10.68. Found C, 53.48, 53.48; H, 4.80, 4.85; N, 10.84, 10.88.

Example 39

[0107] Yellow solid; HPLC: 2.47 min; Anal. Calcd. for C34H36N6O12-1.0H2O-0.5CF3CO2H C, 52.83; H, 4.87; N, 10.56. Found C, 52.99, 53.30; H, 4.80, 4.83; N, 10.87, 10.84.

[0108] Table 2 shows compounds of the invention in accord with structural diagram VIII, having 2-carboxy-linked peptides.

2TABLE 2Example No.AA1AA2AA3AA4AA540GlyGlyProGluGly41GluGlyProGluGly42ArgGlyProGluGly43GlyGluProGluGly44GluGluProGluGly45ArgGluProGluGly46GlyArgProGluGly47GluArgProGluGly48ArgArgProGluGly49GlyGlyProArgGly50GluGlyProArgGly51ArgGlyProArgGly52GlyGluProArgGly53GluGluProArgGly54ArgGluProArgGly55GlyArgProArgGly56GluArgProArgGly57ArgArgProArgGly

Example 40

[0109] Yellow solid: HPLC; 2.77 min; Anal. Calc. For C31H31N5O11-1.0OH2O-0.5CF3CO2H C, 53.04; H, 4.66; N, 9.66. Found: C, 53.10, 52.99; H, 4.68, 4.61; N, 9.87, 9.93.

Example 41

[0110] Yellow solid; HPLC: 2.83 min; Anal. Calc. For C34H35N5O13 -0.5H2O-0.5CF3CO2H C, 53.37; H, 4.67; N, 8.89. Found: C, 53.35, 53.46; H, 4.75, 4.83; N, 8.85, 8.89 min.

Example 42

[0111] Yellow solid; HPLC: 2.74 min; Anal. Calc. For C35H40N8O11-0.5H2O-1.5CF3CO2H C, 49.14; H, 4.61; N, 12.06. Found: C, 49.07, 49.12; H, 4.64, 4.70; N, 12.06, 12.08.

Example 43

[0112] Yellow solid; HPLC: 2.78 min; Anal. Calc. For C34H35N5O13-0.75H2O-0.4CF3CO2H C, 53.53; H, 4.76; N, 8.97. Found: C, 53.75, 53.75; H, 4.80, 4.79; N, 9.04, 9.06.

Example 44

[0113] Yellow solid; HPLC: 2.83 min; Anal. Calc. For C37H39N5O15-0.5H2O-0.5CF3CO2H C, 53.09; H, 4.75; N, 8.15. Found: C, 52.92, 52.86; H, 4.94, 4.88; N, 8.22, 8.22.

Example 45

[0114] Yellow solid; HPLC: 2.74 min; Anal. Calc. For C38H44N8O13-1.5H2O-1.4CF3CO2H C, 48.64; H, 4.84; N, 11.12. Found: C, 48.36, 48.46; H, 4.68, 4.71; N, 11.23, 11.28.

Example 46

[0115] Yellow solid; HPLC: 2.49 min; Anal. Calc. For C35H40N8O11-0.5H2O-1.5CF3CO2H C, 49.14; 4.61; N, 12.06. Found: C, 49.00, 48.67; H, 4.68, 4.67; N, 12.24, 12.14.

Example 47

[0116] Yellow solid; HPLC: 2.83 min; Anal. Calc. For C38H44N8O13-1.0H2O-1.5CF3CO2H C, 48.76; H, 4.74; N, 11.10. Found: C, 48.69, 48.96; H, 4.74, 4.76; N, 11.33, 11.34.

Example 48

[0117] Yellow solid; HPLC: 2.48 min; Anal. Calc. For C39H49N11O11-2.0H2O-2.2CF3CO2H C, 45.94; H, 4.90; N, 13.58. Found: C, 45.72, 45.90; H, 4.61, 4.62; N, 13.75, 13.79. C, 45.94; H, 4.90; N, 13.58. Found: C, 45.72 ,45.90; H, 4.61, 4.62; N, 13.75, 13.79.

Example 49

[0118] Yellow solid; HPLC: 2.74 min; Anal. Calc. For C32H36N8O9-0.5H2O-1.5CF3CO2H C, 49.07; H, 4.53; N, 13.08. Found: C, 49.06, 49.10; H, 4.53, 4.54; N, 13.30, 13.29.

Example 50

[0119] Yellow solid; HPLC: 2.84 min; Anal. Calc. For C35H40N8O11-0.5H2O-1.5CF3CO2H C, 49.14; H, 4.61; N, 12.06. Found: C, 48.87, 49.00; H, 4.63, 4.64; N, 12.23, 12.29.

Example 51

[0120] Yellow solid; HPLC: 2.49min; Anal. Calc. For C36H45N11O9-1.5H2O-2.5CF3CO2H C, 45.27; H, 4.68; N, 14.16. Found: ,. 45.10, 45.14; H, 4.60, 4.55; N, 14.27, 14.28.

Example 52

[0121] Yellow solid; HPLC: 2.81 min; Anal. Calc. For C35H40N8O11-1.25H2O-1.5CF3CO2H C, 48.44; H, 4.71; N, 11.89. Found: C, 48.40, 48.28; N. 4.62, 4.60; N, 12.13, 12.10.

Example 53

[0122] Yellow solid; HPLC: 2.92 min; Anal. Calc. For C38H44N8O13-1.0H2O-1.5CF3CO2H C, 48.76; H, 4.74; N, 11.10. Found: C, 48.36, 48.52; H, 4.67, 4.69; N, 11.20, 11,26.

Example 54

[0123] Yellow solid; HPLC: 2.56 min; Anal. Calc. For C39H49N11O11-1.5H2O-2.3CF3CO2H C, 46.05; H, 4.81; N, 13.55. Found: C, 45.83, 45.90; H, 4.63, 4.63; N, 13.79, 13.73.

Example 55

[0124] Yellow solid; HPLC: 2.57 min; Anal. Calc. For C36H45N11O9-2.0H2O-2.2CF3CO2H C, 45.66; H, 4.86; N, 14.50. Found: C, 45.46, 45.58; H, 4.57, 4.58; N, 14.57, 14.61.

Example 56

[0125] Yellow solid; HPLC: 2.71 min; Anal. Calc. For C39H49N11O11-1.5H2O-2.3CF3CO2H C, 46.05; H, 4.81; N, 13.55. Found: C, 46.03, 45.83; H, 4.68, 4.65; N, 13.74, 13.72.

Example 57

[0126] Yellow solid; HPLC: 2.09 min; Anal. Calc. For C40H54N14O9-2.5H2O-3.2CF3CO2H C, 43.37; H, 4.89; N, 15.26. Found: C, 43.40, 43.28; H, 4.57, 4.52; N, 15.38, 15.34.

Example 58

[0127] A Compound in Accord with Structural Diagram IX:

[0128] A compound in accord with structural diagram IX having the N-Ac-amino acid sequence N-Ac-Glu-Gly-Gln was synthesized as follows: FMOCAsp(OH)OtBu (3.3 g, 8 mmol) and N-methylmorpholine (900 μL, 8 mmol) were dissolved in dry THF (20 mL) and chilled to −10° C. Isobutylchloroformate (910 μL, 7 mmol) was added dropwise, maintaining reaction temperature below −7° C. To this mixture was added a suspension of 2-amino-phenanthrene-9,10-dione (1.12 g, 5 mmol) in dry THF (40 mL), and the reaction was allowed to warm to ambient temperature. The disappearance of 2-aminophenanthrene-9,10-dione was monitored by TLC (Rf=0.42, 25:75 EtOAc:CH2Cl2, v/v on a silica gel plate), and reaction was complete after 7 d. The solvent was removed by rotary evaporation and the residue was partitioned between EtOAc and sat'd. aq. NH4Cl. The organic phase was washed with sat'd. aq. NaHCO3 and brine, then dried over anhydrous MgSO4. This mixture was filtered and the filtrate was concentrated to a red-brown solid and chromatographed on silica gel with 15:85, EtOAC:CH2Cl2, v/v. This gave FMOCAsp-2-aminophenanthrene-9,10-dione (1.92 g, 3.1 mmol, 63%) as a red solid. This intermediate was dissolved in CH2Cl2(100 mL) and TFA (30 mL) was added; after 1h the volatiles were removed by rotary evaporation. Residual TFA was removed by dissolving the material in CH2Cl2 and concentration on the rotary evaporator twice. This was dissolved in dry CH2Cl2 (100 mL), diisopropylethylamine (5 mL) and dry DMF (10 mL) were added under N2. 2-chlorochlortrityl resin (4.5 g of 1.2 mmol/g, 5.5 mmol) was added to the mixture and mixed by shaking. After 3 h, MeOH (10 mL) was added and the shaking was continued for an additional 10 min. The resin was collected by vacuum filtration and washed with CH2Cl2 (5×10 mL), DMF (2×10 mL), CH2Cl2 (2×10 mL) and MeOH (3×10 mL). The resin was dried for 64 h in vacuo. An incorporation of 2.3 mmol of FMOCAsp(3-aminophenanithrene-9,10-dione) was calculated based on the increase of mass to 5.8 g. The resin was swelled in DMF (25 mL) for 1 h, then washed with DMF (5×25 mL). The resin was treated with 20% piperidine in DMF (10 mL) for 3 min, drained, then was treated with more 20% piperidine in DMF (10 mL) for 7 min and drained. The resin was washed with DMF (10×10 mL). A solution of FMOCGln(trt)OH (6.72 g, 11 mmol), HATU (3.8 g, 10 mmol), and diisopropylethylamine (3.5 mL) in DMF (15 mL) was added to the resin and mixed with gentle nitrogen bubbling for 2 h. The solution was drained and the resin was washed with DMF (10×10 mL). The resin was treated with 10 ml 20% piperidine in DMF (10 mL) for 3 min, drained, then another portion of 20% piperidine in DMF (10 mL) was added for 7 min and drained. The resin was washed with DMF (10×10 mL). A portion of the resin (ca. 10%) was removed for other reactions and the remaining resin was treated with a solution of FMOCGlyOH (1.93 g, 11 mmol), HATU (3.8 g, 10 mol), diisopropylethylamine (3.5 mL) in DMF (15 mL) for 1 h. The reaction was drained and washed with DMF (10×10 mL). The resin was treated with 20% piperidine in DMF (10 mL) for 3 min, drained, then treated with additional 20% piperidine in DMF (10 mL) for 7 min and drained. The resin was washed with DMF (10×10 mL). A portion of the resin (about 10%) was removed for other reactions and the remaining resin was treated with a solution of FMOCGlu(tBu)OH (4.68 g, 11 mmol). HATU (3.8 g, 10 mmol). and diisopropylethylamine (3.5 mL) in DMF (15 mL) for 16 h. The solution was drained and the resin was washed with DMF (10×10 mL). The resin was treated with 20% piperidine in DMF (10 mL) for 3 min, drained, then additional 20% piperidine in DMF (10 mL) was added for 7 min and drained. The resin was washed with DMF (10×10 mL). A portion of this material (ca. 15%) was removed and treated with a solution of Ac2O (1 mL) and diisopropylethylamine (2 mL) in DMF (5 mL) for 2 h. The solution was drained and washed withDMF (10×5 mL), CH2Cl 2 (5×5 mL), Et2O (5×5 mL) and dried under a N2 stream. The resin was treated with a solution of TFA containing 2% H2O and 2% thioanisole (5 mL) for 2 h. The solids were removed and the solution was concentrated to a red oil. Addition of Et2O (10 mL) induced precipitation of a red solid, which was collected by vacuum filtration. The material was purified by preparative HPLC on a C18 Dynamax column (21.4 mm×25 cm, 60 Å) using a gradient of 10% to 26% acetonitrile in H2O with 0.1% TFA. Fractions containing pure product were combined and lyophilized to give the product (40 mg) as a red solid. HPLC: 2.69 min; HRMS theor. [M+H]:695.2313 amu; obs. [M+H] 695.2307 amu; deviation of −0.9 ppm.

[0129] Table 3 shows compounds of the invention in accord with structural diagram IX, made by the method of Example 58, utilizing the appropriate peptide fragments.

3TABLE 3Example No.amino acid sequence58N—Ac-Glu-Gly Gin59N—Ac-Gln60N—Ac-Ala-Thr-Glu-Gly-Gln61N—Ac-Thr-Ala-Thr-Glu-Gly-Gln62N—Ac-Phe-Thr-Ala-Thr-Glu-Gly-Gln

Example 59

[0130] Red Solid; HPLC: 2.85 min; HRMS theor. [M+H]: 509.1672 amu; obs. [M+H] 509.1657 amu; Deviation of −3 ppm.

Example 60

[0131] Red Solid; HPLC: 2.69 min; HRMS theor. [M+H]: 867.3161 amu; obs. [M+H] 867.3145 amu; Deviation of −1.8 ppm.

Example 61

[0132] Red Solid; HPLC: 2.69 min; HRMS theor. [M+H]: 968.3638 amu; obs. [M+H] 968.3619; Deviation of −1.9 ppm.

Example 62

[0133] Red Solid; HPLC: 3.81 min; HRMS theor. [M+H]: 1115.4322 amu; obs. [M+H] 1115.4286 amu; Deviation of −3.2 ppm.

Example 63

[0134] N-(9,10-Dioxo-9,10-dihydro-phenanthren-4-yl)-succinamic Acid Methyl Ester:

[0135] Using a BOHDAN Automated RAM Synthesizer; to a solution 4-amino-phenanthrene-9,10-dione (50 mg, 238 μmol) in THF (10 mL) an excess of Na2CO3 (1 g) was added, followed by 3-carbomethoxypropionyl chloride (381 μmol; 323 μL). The reaction cycle, which consisted of vortexing the mixture twice for 10 s, was repeated 10 times, and this was followed by filtering and evaporating the solvent. The reaction mixture was then purified on a 10 g silica gel column, using CH2Cl2-5% EtOAC/CH2Cl2-10% EtOAC/CH2Cl2-20% EtOAC/CH2Cl2 as the eluant, to yield the pure N-(9,10-Dioxo-9.10-dihydro-phenanthren-4-yl)-succinamic acid methyl ester as a brown solid. 1H NMR (300 MHz, DMSO-d6) δ10.26 1H, s), 8.43 (1H, d, J=7.8 Hz), 8.01 (1H, dd, J=1.4, 7.8 Hz), 7.92 (1H, dd, J=1.4, 7.4 Hz). 7.75 (1H, ddd, J=1.5, 7.8, 7.8 Hz), 7.68 (1H, dd, J=1.5, 7.8 Hz), 7.53 (1H, d, J=7.8 Hz), 7.50 (1H, d, J=7.5 Hz), 3.62 (3H, d), 2.67 (4H, m); HPLC: 3.57 min.

[0136] The compounds of examples 64 to 79 inclusive were prepared by the method of Example 63, utilizing the appropriate acid chloride.

Example 64

[0137] 7-(5-Nitro-9,10-dioxo-9,10-dihydro-phenanthren-2-ylcarbamoyl)-heptanoic Acid Methyl Ester:

[0138] Brown solid; 1H NMR (300 MHz, DMSO-d6) δ10.37 (1H, s), 8.39 (1H, d, J=2.4 Hz), 8.22 (1H, dd, J=1.5, 8.0 Hz), 8.11 (1H, dd, J=1.2, 8.3 Hz), 7.85 (1H, dd, J=2.7, 9.0 Hz), 7.66 (1H, dd, J=7.8, 7.8 Hz), 7.38 (1H, d, J=8.6 Hz), 3.58 (3H, s), 2.38-2.27 (4H, m), 1.62-1.48 (4H, m), 1.30 (4H, m); HPLC: 7.48 min.

Example 65

[0139] 4-(5-Nitro-9,10-dioxo-9,10-dihydro-phenanthren-2-ylcarbamoyl)-butyric Acid Methyl Ester:

[0140] Red Solid; 1H NMR (300 MHz, DMSO-d6) δ10.41 (1H, s), 8.39 (1H, d, J=2.4 Hz), 8.22 (1H, d, J=1.1, 7.8 Hz), 8.11 (1H, dd, J=1.2, 8.0 Hz), 7.84 (1H, dd, J=2.6, 9.0Hz), 7.66 (1H, dd, J=7.9, 7.9 Hz), 7.38 (1H, d, J=9.0 Hz), 3.60 (3H, s), 2.42 (2H, t, J=7.4 Hz), 2.39 (2H, t, J=7.4 Hz), 1.86 (2H, tt, J=7.7, 7.7 Hz); HPLC: 5.96 min.

Example 66

[0141] N-(5-Nitro-9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-succinamic Acid Methyl Ester:

[0142] Red Solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.51 (1H, s), 8.39 (1H, d, J=2.3 Hz), 8.22 (1H, dd, J=0.8, 7.9 Hz), 8.11 (1H, dd, J=1.1, 7.9 Hz), 7.82 (1H, dd, J=2.3, 8.7 Hz), 7.66 (1H, dd, J=7.6, 7.6 Hz), 7.38 (1H, d, J=8.6 Hz), 3.61 (3H, s), 2.66 (4H, m); HPLC: 5.58 min.

Example 67

[0143] 4-(9,10-Dioxo-9,10-dihydro-phenanthren-4-ylcarbamoyl)-butyric Acid Methyl Ester:

[0144] Orange solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.18 (1H, s), 8.35 (1H, d, J=9 Hz), 7.99 (1H, d, J=6 Hz), 7.93 (H, d, J=9 Hz), 7.75-7.67 (2H, s), 7.53 (1H, d, J=6 Hz), 7.50 (1H, d, J=9 Hz), 3.62 (3H, s), 2.44-2.37 (4H, m), 1.91-1.82 (2H, m), HPLC: 3.89 min.

Example 68

[0145] 7-(9,10-Dioxo-9,10-dihydro-phenanthren-4-ylcarbamoyl)-heptanoic Acid Methyl Ester:

[0146] Orange solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.14 (1H, s), 8.35 (1H, d, J=8.0 Hz), 7.99 (1H, d, J=7.6 Hz), 7.92 (1H, d, J=7.7 Hz), 7.75-7.66 (2H, m), 7.51 (2H, m), 3.59 (3H, s), 2.37 (2H, t, J=7.2 Hz), 2.31 (2H, t, J=7.6 Hz), 1.58 (4H, m), 1.33 (4H, m); HPLC: 5.81 min.

Example 69

[0147] 9-(9,10-Dioxo-9,10-dihydro-phenanthren-4-ylcarbamoyl)-nonanoic Acid Methyl Ester:

[0148] Brown solid; 1H NMR (300 MHz; DMSO-d6 ) δ10.12 (1H, s), 8.35 (1H, d, J=8.5 Hz), 7.99 (1H, d, J=7.7 Hz), 7.92 (1H, d, J=7.6 Hz), 7.70 (2H, m), 7.52 (1H, d, J=7.6 Hz), 7.50 (1H, d, J=7.7 Hz), 3.58 (3H, s), 2.37 (2H, t, J=7.0 Hz), 2.29 (2H, t, J=7.5 Hz), 1.61 (2H, m), 1.52 (2H, m), 1.29 (8H, m); HPLC: 7.31 min.

Example 70

[0149] 4-(9,10-Dioxo-9,10-dihydro-phenanthren-2-ylcarbamoyl)-butyric Acid Methyl Ester:

[0150] Brown solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.27 (1H, s), 8.28 (1H, d, J=2.5 Hz), 8.23 (1H, d, J=8.9 Hz), 8.19 (1H, d, J=8.1 Hz), 7.99 (1H, d, J=7.3 Hz), 7.94 (1H, d, J=2.0 Hz), 7.75 (1H, dd, J=7.7, 7.7 Hz), 7.47 (1H, dd, J=7.5, 7.5 Hz), 3.60 (3H, s), 2.40 (4H, m), 1.87 (2H, tt, J=7.4, 7.4 Hz); HPLC: 5.58 min.

Example 71

[0151] 7-(9,10-Dioxo-9,10-dihydro-phenanthren-2-ylcarbamoyl)-heptanoic Acid Methyl Ester:

[0152] Brown solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.22 (1H, s), 8.28 (1H, d, J=2.4 Hz), 8.20 (2H, m), 7.98 (2H, m), 7.75 (1H, dd, J=7.3, 7.3 Hz), 7.47 (1H, dd, J=7.7, 7.7 Hz), 3.30 (3H, s), 2.37-2.28 (4H, m), 1.63-1.52 (4H, m), 1.31 (4H, m); HPLC: 7.26 min.

Example 72

[0153] 9-(9,10-Dioxo-9,10-dihydro-phenanthren-2-ylcarbamoyl)-nonanoic Acid Methyl Ester:

[0154] Brown solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.22 (1H, s), 8.28 (1H, d, J=2.0 Hz), 8.22 (1H, d, J=8.9 Hz), 8.19 (1H, d, J=8.2 Hz), 7.98 (2H, m), 7.75 (1H, dd, J=7.3, 7.3 Hz), 7.47 (1H, dd, J=7.7, 7.7 Hz), 3.57 (3H, s), 2.34 (2H, t, J=7.2 Hz), 2.28 (2H, t, J=7.3 Hz), 1.61 (2H, m), 1.52 (2H, m); HPLC: 8.57 min.

Example 73

[0155] N-[5-(3-Methoxycarbonyl-propionylamino)-9,10-dioxo-9,10-dihydro-phenanthren-2-yl]-succinamic Acid Methyl Ester:

[0156] Pink solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.40 (1H, s), 10.18 (1H, s), 8.39 (1H, d, J=8.7 Hz), 8.23 (1H, d, J=2.0 Hz), 7.90 (2H, m), 7.63 (1H, d, J=7.9 Hz), 7.45 (1H, dd, J=7.7, 7.7 Hz), 3.62 (3H, s), 3.61 (3H, s), 2.65 (8H, m); HPLC: 3.64 min.

Example 74

[0157] 4-[5-(4-Methoxycarbonyl-butyrylamino)-9,10-dioxo-9,10-dihydro-phenanthren-2-ylcarbamoyl]-butyric Acid Methyl Ester:

[0158] Brown solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.31 (1H, s), 10.11 (1H, s), 8.29 (1H, d, J=8.8 Hz), 7.91 (2H, m), 7.64 (1H, d, J=7.2 Hz), 7.47 (1H, dd, J=7.7, 7.7 Hz), 3.63 (3H, s), 3.60 (3H, s), 2.40 (8H, m), 1.87 (4H, m); HPLC: 4.24 min.

Example 75

[0159] 7-[5-(7-Methoxycarbonyl-heptanoylamino)-9,10-dioxo-9,10-dihydro-phenanthren-2-ylcarbamoyl]-heptanoic Acid Methyl Ester:

[0160] Brown solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.23 (1H, s), 10.03 (1H, s), 8.29 (1H, d, J=8.9 Hz), 8.20 (1H, s), 7.90 (2H, m), 7.63 (1H, d, J=8.1 Hz), 7.44 (1H, dd, J=7.7, 7.7 Hz), 3.58 (6H, s), 2.31 (8H, m), 1.56 (8H, m), 1.30 (8H, m); HPLC: 7.09 min.

Example 76

[0161] 9-[5-(9-Methoxycarbonyl-nonanoylamino)-9,10-dioxo-9,10-dihydro-phenanthren-2-ylcarbamoyl]-nonanoic Acid Methyl Ester:

[0162] Brown solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.22 (1H, s), 10.03 (1H, s), 8.29 (1H, d, J=8.6 Hz), 8.20 (1H, s), 7.93 (1H, dd, J=2.0, 8.9 Hz), 7.89 (1H, d, J=7.6 Hz), 7.62 (1H, d, J=7.6 Hz), 7.44 (1H, dd, J=7.7, 7.7 Hz), 3.57 (6H, s), 2.38-2.26 (8H, m), 1.62-1.50 (8H, m), 1.28 (16H, m); HPLC: 9.21 min.

Example 77

[0163] 4-(9,10-Dioxo-9,10-dihydro-phenanthren-3-ylcarbamoyl)-butyric Acid Methyl Ester:

[0164] Orange solid: 1H NMR (300 MHz, DMSO-d6 ) δ10.46 (1H, s), 8.53 (1H, d, J=1.7 Hz), 8.04-7.98 (3H, m), 7.83 (1H, dd, J=7.3, 7.3 Hz), 7.67 (1H, dd, J=1.5, 8.8 Hz), 7.56 (1H, dd, J=7.2, 7.2 Hz), 3.61 (3H, s), 2.45 (4H, m), 1.89 (2H, tt, J=7.7, 7.7 Hz); HLPC: 5.68 min.

Example 78

[0165] 8-(9,10-Dioxo-9,10-dihydro-phenanthren-3-ylcarbamoyl)-heptanoic Acid Methyl Ester:

[0166] Orange solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.42 (1H, s), 8.54 (1H, d, J=1.6 Hz), 8.04-7.98 (3H, m), 7.83 (1H, ddd, J=1.2, 7.6, 7.6 Hz), 7.68 (1H, dd, J=1.4, 8.6 Hz), 7.56 (1H, dd, J=7.4, 7.4 Hz), 3.58 (3H, s), 2.40 (2H,t, J=7.3 Hz), 2.30 (2H, t, J=7.3 Hz), 1.63 (2H, m), 1.54 (2H, m), 1.32 (4H, m); HPLC: 7.44 min.

Example 79

[0167] 9-(9,10-Dioxo-9,10-dihydro-phenanthren-3-ylcarbamoyl)-nionanoic Acid Methyl Ester:

[0168] Brown solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.42 (1H, s), 8.54 (1H, s), 8.02 (3H, m), 7.84 (1H, dd, J=7.7, 7.7 Hz), 7.69 (1H, d, J=8.7 Hz), 7.56 (1H, dd, J=7.7, 7.7 Hz), 3.57 (3H, s), 2.40 (2H, t, J=7.3 Hz), 2.28 (2H, t, J=7.3 Hz), 1.63 (2H, m), 1.52 (2H, m), 1.29 (8H, m); HPLC: 8.82 min.

[0169] Compounds of examples 80 and 81 were made as follows: To a solution of 2-amino-phenanthrene-9,10-dione (670 mg, 3.0 mmol) in THF (70 mL) diisopropylethylamine (1.1 mL, 6.0 mmol) was added, followed by methyl 4-chloro-4-oxobutyrate for Example 81 N-(9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-succinamic acid methyl ester (490 μL, 4.0 mmol) or acetyl chloride for Example 82, N-(9,10-dioxo-9,10-dihydro-phenanthren-2-yl)-acetamide (284 μL, 4.0 mmol), Each reaction was stirred for 2.5 h, then the THF was removed by rotary evaporation, and the reaction mixture was chromatographed on silica gel (9:1, CH2Cl2: MeOH, v/v) and the product eluted.

Example 80

[0170] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-succinamic Acid Methyl Ester:

[0171] Brown solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.39 (1H, s), 8.29 (1H, d, J=2.3 Hz), 8.23 (1H, d, J=9.1 Hz), 8.19 (1H, d, J=8.0 Hz),7.99 (1H, dd, J=1.4, 7.8 Hz), 7.92 (1H, dd, J=2.3, 8.7 Hz), 7.74 (1H, ddd, J=1.1, 7.7, 7.7 Hz), 7.47 (1H, dd, J=7.1, 7.1 Hz), 3.61 (3H, s), 2.65 (4H, m); HPLC: 4.61 min.

Example 81

[0172] N-(9,10-Dioxo-9,10-dihydro-phenanthren-2-yl)-acetamide:

[0173] Purple solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.33 (1H, s), 8.26 (1H, d, J=2.7 Hz), 8.23 (1H, d, J=9.1 Hz), 8.19 (1H, d, J=8.0 Hz), 7.99 (1H, dd, J=1.1, 7.7 Hz), 7.95 (1H, dd, J=2.7, 8.5 Hz), 7.75 (1H, ddd, J=1.6, 8.0, 8.0 Hz), 7.47 (1H, dd, J=7.2, 7.2 Hz), 2.10 (3H, s); HPLC: 4.42 min.

Example 82

[0174] N-[7-(3-Methoxycarbonyl-propionylamino)-9,10-dioxo-9,10-dihydro-phenanthren-2-yl]-succinamic Acid Methyl Ester:

[0175] The compound of example 82 was synthesized by a method that differed from that of example 80 only in that double the amount of acid chloride, methyl 4-chloro-4-oxobutyrate, was used and 2,7-diamino-phenanthrene-9,10-dione was used in place of 2-amino-phenanthrene-9,10-dione. Purple solid; 1H NMR (300 MHz, DMSO-d6 ) δ10.34 (2H, s), 8.25 (2H, d, J=1.9 Hz), 8.10 (2H, d, J=9.0 Hz), 7.89 (2H, dd, J=1.7, 8.4 Hz), 3.61 (6H, s), 2.50 (8H, m); HPLC: 4.78 min.

Example 83

[0176] 2,7-Diamino-phenanthrene-9,10-dione:

[0177] 2,7-Dinitro-phenanthrene-9,10-dione (3.0 g, 10 mmol) was suspended in THF (200 mL) and Raney Nickel (ca. 1 g) was added. The reaction was hydrogenated on a Parr shaker under 50 psi H2 for 2.5 h, at which time the mixture was filtered through a plug of diatomaceous earth and the filtrate was evaporated under rotary evaporation. The product was chromatographed on silica gel (15% to 100% EtOAc in CH2Cl2 as eluant) and dried to afford 2,7-diamino-phenanthrene-9,10-dione (1.1 g, 4.6 mmol, 46%) as a black solid. 1H NMR (300 MHz, TFA-d1-DMSO-d6 ) δ8.77 (2H, d, J=2.7 Hz), 8.76 (2H, d, J=9.1 Hz), 8.62 (2H, dd, J=2.7, 9.1 Hz), Anal. Calcd for C14H6N2O6 C, 56.39; H, 2.02; N, 9.39 Found: C, 56.20, 56.44; H, 2.17, 2.14; N, 8.89, 8.95.

Examples 84 and 85

[0178] Compounds of examples 84 and 85 were made by the following method: 2,5-dinitro-phenanthrene-9,10-dione (3.0 g, 10 mmol) was dissolved in THF (250 mL) and Raney Nickel (ca. 0.3 g) was added. The reaction was hydrogenated on a Parr shaker under 50 psi H2 until the uptake of H2 slowed (ca. 5 h). The reaction mixture was filtered through celite and the filtrate evaporated by rotary evaporation. The reaction mass was a mixture of 2-amino-5-nitro-phenanthrene-9,10-dione and 2,5-diamino-phenanthrene-9,10-dione, and was chromatographed on silica gel.

[0179] The compound of example 84, 2-amino-5-nitro-phenanthrene-9,10-dione was eluted with EtOAc:CH2Cl2 1:4, and dried to afford a black solid (400 mg, 1.7 mmol, 17%), 1H NMR (300 MHz, DMSO-d6 ) δ8.11 (1H, dd, J=1.4, 8.1 Hz), 7.99 (1H, dd, J=1.1, 8.1 Hz), 7.47 (1H, dd, J=7.5, 7.5 Hz), 7.25 (1H, d, J=2.8 Hz), 7.08 (1H, d, J=8.6 Hz), 6.82 (1H, dd, J=2.8, 8.6 Hz), 6.18 (2H, s).

[0180] The compound of example 85, 2,5-diamino-phenanthrene-9,10-dione was eluted with EtOAc and dried to afford a black solid (1.2 g, 5 mmol, 50%), 1H NMR (300 MHz, DMSO-d6) δ8.20 (1H, d, J=8.7 Hz), 7.25 (1H, dd, J=1.5, 7.2 Hz), 7.15 (1H, d, J=2.5 Hz) 7.12-7.01 (2H, m), 6.88 (1H, dd, J=2.5, 8.5 Hz), 5.71 (2H, s), 5.46 (2H, s); HPLC: 1.89 min.

[0181] The compounds of Examples 86 to 89 inclusive were prepared by the method of Example 83 using the appropriate nitrated 9,10-phenanthrenedione as the starting material.

Example 86

[0182] 1-Amino-phenanthrene-9,10-dione:

[0183] Purple solid; 1H NMR (300 MHz, TFA-d1-DMSO-d6) δ8.25 (1H, d, J=8.2 Hz), 8.05 (1H, dd, J=1.2, 7.8 Hz), 7.77 (1H, ddd, J=1.5, 7.2, 8.6 Hz), 6.97 (1H, dd, J=2.1, 7.4 Hz); HPLC: 5.13 min.

Example 87

[0184] 2-Amino-phenanthrene-9,10-dione:

[0185] Black solid; 1H NMR (300 MHz, DMSO-d6) δ8.00 (1H, d, J=7.8 Hz), 7.92-7.87 (2H, m), 7.65 (1H, ddd, J=1.7, 8.0, 8.0 Hz), 7.31 (1H, dd, J=7.0, 7.0Hz), 7.19 (1H, d, J=2.8 Hz), 6.91 (1H, dd, J=2.5, 8.5 Hz), 5.87 (2H, s), Anal. Calcd for C14H9NO2-0.33 H2O C, 73.35 4.25 N, 6.11. Found: C, 73.33, 73.39; H, 4.22, 4.21; N, 6.28, 6.29.

Example 88

[0186] 3-Amino-phenanthrene-9,10-dione:

[0187] Brown solid; 1H NMR (300 MHz, DMSO-d6) δ7.99 (2H, m), 7.83 (1H, d, J=8.4 Hz), 7.78 (1H, d, J=7.2 Hz), 7.53 (1H, dd, J=7.2, 7.2 Hz), 7.30 (1H, s), 6.89 (2H, s), 6.64 (1H, dd, J=1.5, 8.6 Hz), Anal. Calcd, For C14H9O2N-0.10 H2O C, 74.72; H, 4.12; N, 6.22 Found: C, 74.94, 74.74; H, 4.10, 4.08; N, 6.26, 6.30.

Example 89

[0188] 4-Amino-phenanthrene-9,10-dione:

[0189] Black solid; 1H NMR (300 MHz, DMSO-d6) δ8.56 (1H, d, J=8.0 Hz), 7.94 (1H, dd, J=1.4, 7.8 Hz), 7.73 (1H, ddd, J=1.6, 7.8, 7.8 Hz), 7.41 (1H, ddd, J=1.0, 7.9, 7.9 Hz), 7.36 (1H, dd, J=3.2, 5.9 Hz), 7.20 (2H, m), 5.84 (2H, s), Anal. Calcd. For C14H9NO2 C, 75.33; H, 4.06; N, 6.27 Found: C, 75.12, 75.12; H, 4.28,4.29; N, 6.21, 6.20.

Example 90

[0190] N-(9,10-Dioxo-9,10-dihydro-phenanthren-3-yl)-succinamic Acid Methyl Ester:

[0191] To a solution of 3-amino-phenanthrene-9,10-dione (400 mg, 1.8 mmol) in dioxane (100 mL) was added a large excess of Na2CO3 followed by methyl 4-chloro-4-oxobutyrate (240 μL, 2.0 mmol). The reaction was stirred for several hours, then filtered and the solvent was removed under reduced pressure. The material was chromatographed on silica gel with 20% EtOAc in CH2Cl2 as eluant and dried to give N-(9,10-Dioxo-9,10-dihydro-phenanthren-3-yl)-succinamic acid methyl ester as an orange solid. 1H NMR (300 MHz, DMSO-d6) δ10.60 (1H, s), 8.55 (1H, d, J=1.5 Hz), 8.04-7.98 (3H, m), 7.84 (1H, ddd, J=1.6, 7.9, 7.9 Hz), 7.65 (1H, dd, J=2.0, 8.7 Hz), 7.56 (1H, dd, J=7.6, 7.6 Hz), 3.62 (3H, s), 2.72 (2H, m), 2.67 (2H, m); HPLC: 5.30 min.

Example 91

[0192] N-(9,10-Dioxo-9,10-dihydro-phenanthren-1-yl)-succinamic Acid Methyl Ester:

[0193] To a solution of 1-amino-phenanthrene-9,10-dione (50 mg, 240 μmol) in THF (50 mL) was added Na2CO3 (210 mg, 2.2 mmol), followed by methyl 4-chloro-4-oxobutyrate (60 μL, 500 μmol). The mixture was stirred overnight filtered, and diluted with ethyl acetate. The organic layer was washed with water, brine, dried over Mg2SO4, filtered, and chromatographed on silica gel with ethyl acetate as the eluant to yield N-(9,10-dioxo-9,10-dihydro-phenanthren-1-yl)-succinamic acid methyl ester as an orange solid. 1H NMR (300 MHz, DMSO-d6) δ12.04 (1H, s), 8.60 (1H, d, J=7.7 Hz), 8.32 (1H, d, J=7.7 Hz), 8.03 (2H, m), 7.79 (2H, m), 7.56 (1H, dd, J=7.2, 7.2 Hz), 3.61 (1H, dd, J=7.2 Hz), 2.81 (2h, m) 2.69 (2H, m); HPLC: 6.49 min.

Examples 92 to 95

[0194] Compounds of examples 92 to 95 inclusive were prepared substantially in accordance with the procedures disclosed in Schmidt, J. Chem. Ber. 1903, 23, 3726-3730, taking into account the results disclosed by Ray, F. E.; Francis, W. C. J. Org. Chem. 1943, 8, 52-59, which procedures and results are incorporated herein by reference.

Example 92

[0195] 2-Nitro-phenanthrene-9,10-dione:

[0196] Orange solid; 1H NMR (300 MHz, DMSO-d6) δ8.80 (1H, d, J=2.8 Hz), 8.69 (1H, d, J=8.5 Hz), 8.60 (1H, dd, J=2.4, 8.7 Hz), 8.51 (1H, d, J=8.31 Hz), 8.22 (1H, dd, J=1.4, 7.7 Hz), 7.93 (1H, ddd, J=1.6, 8.1, 8.1 Hz), 7.73 (1H, dd, J=7.5, 7.5 Hz). Anal. Calcd. For C14H7NO4: C, 66.41; H, 2.79; N. 5.53; Found: C, 66.70, 66.71; H, 2.83, 2.84; N. 5.57, 5.63.

Example 93

[0197] 4-Nitro-phenanthrene-9,10-dione:

[0198] Orange solid; 1H NMR (300 MHz, DMSO-d6) δ8.27 (1H, dd, J=1.5, 7.9 Hz), 8.17 (1H, dd, J=1.3, 7.9 Hz), 8.10 (1H, dd, J=1.5, 7.8 Hz), 7.79-7.71 (2H, m), 7.63 (1H, ddd, J=0.9, 7.7, 7.7 Hz), 7.46 (1H, d, J=8.0 Hz), Anal. Calcd. For C14H7NO4-0.25 H2O C, 65.25; H, 2.93; N, 5.43. Found: C, 65.19, 65.24; H, 2.82. 2.83; N, 5.43, 5.44.

Example 94

[0199] 2,7-Dinitro-phenanthrene-9,10-dione:

[0200] Yellow solid; 1H NMR (300 MHz, tfashake-DMSO-d6) δ8.77 (2H, d, J=2.7 Hz), 8.76 (2H, d, J=9.1 Hz), 8.62 (2H, dd, J=2.7, 9.1 Hz). Anal. Calcd. for C14H6N2O6 C, 56.39; H, 2.02; N, 9.39 Found: C, 56.20, 56.44; H, 2.17, 2.14; N, 8.89, 8.95.

Example 95

[0201] 2,5-Dinitro-phenanthrene-9,10-dione:

[0202] Yellow solid; 1H NMR (300 MHz, DMSO-d6) δ8.67 (1H, d, J=2.5 Hz), 8.54 (1H, dd, J=2.5, 9.1 Hz), 8.36 (1H, dd, J=1.5, 8.1 Hz), 8.26 (1H, dd, J=1.3, 7.8 Hz), 7.87 (1H, dd, J=7.8, 7.8 Hz), 7.73 (1H, d, J=8.8 Hz),

Example 96

[0203] 2-Bromo-phenanthrene-9,10-dione:

[0204] This compound was prepared substantially in accordance with the procedures disclosed in Bhatt, M. V.; Tetrahedron 1964, 20, 803-821, which procedures are incorporated herein by reference. Orange solid; 1H NMR (300 MHz, DMSO-d6) δ8.30 (1H, d, J=7.9 Hz), 8.27 (1H, d, J=8.6 Hz), 8.07 (1H, d, J=2.4 Hz), 8.04 (1H, d, J=1.0, 7.6 Hz), 7.96 (1H, dd, J=2.7, 8.9 Hz), 7.79 (1H, ddd, J=1.7, 8.2, 8.2 Hz), 7.56 (1H, dd, J=7.6, 7.6 Hz), Anal. Calcd. For C14H7O2 C, 58.57; H, 2.46. Found: C, 58.22, 58.99; H, 2.63, 2.65.

Examples 97 and 98

[0205] The compounds of examples 97 and 98 were prepared substantially in accordance with the procedures disclosed in Langenbeck, W.; Schaller, R.; Arneberg, K. Chem. Ber. 1942, 75, 1483-1488, which procedures are incorporated herein by reference.

Example 97

[0206] 9,10-Dioxo-9,10-dihydro-phenanthrene-2-carboxylic Acid:

[0207] Yellow solid; 1H NMR (300 MHz, DMSO-d6) δ13.45 (1H, s), 8.51 (1H, d, J=1.9 Hz), 8.46 (1H, d, J=8.2 Hz), 8.37 (1H, d, J=8.0 Hz), 8.24 (1H, dd, J=2.0, 8.2 Hz), 8.08 (1H, dd, J=0.75, 7.6 Hz), 7.83 (1H, dd, J=7.2, 7.2 Hz), 7.61 (1H, dd, J=7.5, 7.5 Hz) Anal calcd. For C15H8O4-0.4 H2O C, 69.45; H, 3.42 Found: C, 69.59, 69.66; H 3.16, 3.17.

Example 98

[0208] 9,10-Dioxo-9,10-dihydro-phenanthrene-3-carboxylic Acid:

[0209] Orange solid; 1H NMR (300 MHz, DMSO-d6) δ13.66 (1H, br s), 8.71 (1H, s), 8.35 (1H, d, J=8.0 Hz), 8.12 (1H, d, J=8.0 Hz), 8.08-8.02 (2H, m), 7.81 (1H, dd, J=7.5, 7.5 Hz), 7.58 (1H, dd, J=7.5, 7.5 Hz), Anal. Calcd. For C15H8O4-0.2 H2O C, 70.42; H, 3.31 Found: C, 70.61, 70.76; H, 3.41, 3.44.

Example 99

[0210] 3-Nitro-phenanthrene-9,10-dione:

[0211] This compound was prepared substantially in accordance with the procedures disclosed in Braithwaite, R. S. W.; Holt, P. F. J. Chem. Soc. 1959, 2304-2305, which procedures are incorporated herein by reference. Orange solid; 1H NMR (300 MHz, TFA-d1-DMSO-d6) δ9.02 (1H, d, J=1.7 Hz), 8.50 (1H, d, J=8.1 Hz), 8.29 (2H, m), 8.13 (1H, dd, J=1.1, 7.6 Hz), 7.85 (1H, ddd, J=1.3, 7.7, 7.7 Hz), 7.64 (1H, dd, J=7.4, 7.4 Hz), Anal. Calcd. For C14H7NO4-0.1H2O: C, 65.94; H, 2.85; N, 5.49. Found: C, 65.78, 66.02; H, 2.92, 2.93; N, 5.41, 5.40.

Examples 100 and 101

[0212] The compounds of examples 100 and 101 were prepared substantially in accordance with the procedures disclosed in Mosettig, E.; Van de Kamp, J. J. Am. Chem. Soc. 1930, 52, 3704-3710, which procedures are incorporated herein by reference.

Example 100

[0213] 3-Acetyl-phenanthrene-9,10-dione:

[0214] Orange solid; 1H NMR (300 MHz, DMSO-d6) δ8.70 (1H, d, J=2.4 Hz), 8.45 (1H, d, J=8.1 Hz), 8.13 (1H, d, J=8.1 hz), 8.07 (1H, dd, J=2.4, 7.8 Hz), 8.02 (1H, dd, J=1.5, 8.1 Hz), 7.82 (1H, ddd, J=2.4, 7.6, 7.6 Hz), 7.58 (1H, ddd, J=1.2, 7.5, 7.5 Hz), 2.75 (3H, s); HPLC: 5.48 min.

Example 101

[0215] 2-Acetyl-phenanthrene-9,10-dione:

[0216] Yellow solid; 1H NMR (300 MHz, DMSO-d6) δ8.49-8.45 (2H, m), 8.39 (1H, d, J=8.1 Hz), 8.27 (1H, dd, J=2.1, 8.4 Hz), 8.08 (1H, dd, J=1.2, 7.8 Hz), 7.83 (1H, ddd=1.5, 7.4, 7.4 Hz), 7.61 (1H, dd, J=7.5 Hz), 2.68 (3H, s); HPLC: 5.27 min.

Example 102:

[0217] To a solution of 9,10-dioxo-9,10-dihydro-phenanthrene-3-carboxylic acid (1.1 g, 4.5 mmol) in anhydrous DMF (10 mL) under N2 was added t-butyl glycine hydrochloride (760 mg, 4.6 mmol), EDC (1.1 g, 6.0 mmol), DMAP (60 mg, 500 μmol) and diisopropylethylamine (2.1 mL, 12 mmol) and the resultant solution was stirred for 16 h. The reaction was diluted with EtOAc and washed with 1M HCI, water, sat'd aq. NaHCO3 and brine. The organic layer was dried over MgSO4, filtered, concentrated, and chromatographed on silica gel (3:1 EtOAc:CH2Cl2, v/v) to afford a yellow solid (500 mg). This solid was subsequently recrystallized from refluxing EtOAc to afford pure [(9,10-dioxo-9,10-dihydro-phenanthrene-3-carbonyl)-amino]-acetic acid tert-butyl ester (310 mg, 31%). Yellow solid; 1H NMR (300 MHz, DMSO-d6) δ9.29 (1H, dd, J=6.1, 6.1 Hz), 8.71 (1H, s), 8.38 (1H, d, J=8.0 Hz), 8.13 (1H, d, J=8.3 Hz), 8.07 (1H, dd, J=1.5, 7.9 Hz), 7.97 (1H, dd, J=1.5, 8.3 Hz), 7.85 (1H, ddd, J=1.5, 7.8, 7.8 Hz), 7.58 (1H, ddd, J=7.2, 7.2 Hz), 4.00 (2H, d, J=6.1 Hz), 1.45 (9H, s); Anal. Calcd. For C21H91NO5-0.2H2O: C, 68.36; H, 5.30; N, 3.80. Found: C, 68.22, 68.58 ; H, 5.17, 5.20; N, 3.87, 3.89.

[0218] The compound of example 103 was prepared by the method of Example 102, utilizing 9,10-dioxo-9,10-dihydro-phenanthrene-2-carboxylic acid as the starting material.

Example 103

[0219] [(9,10-Dioxo-9,10-dihydro-phenanthrene-2-carbonyl)-amino]-acetic acid tert-butyl ester:

[0220] Yellow solid; 1H NMR (300 MHz, DMSO-d6) δ9.23 (1H, dd, J=5.7, 5.7 Hz), 8.54 (1H, d, J=1.9Hz), 8.46 (1H, d, J=8.7Hz), 8.39 (1H, d, J=7.9 Hz), 8.22 (1H, dd, J=2.3, 8.7 Hz), 8.07 (1H, dd, J=1.6, 7.7 Hz). 7.82 (1H, ddd, J=1.5, 7.9, 7.9 Hz). 7.59 (1H, dd, J=7.4, 7.4 Hz), 3.94 (2H, d, J=6.3 Hz), 1.44 (9H, s); Anal. Calcd. For C21H19NO5-0.1 H2O: C, 68.69; H, 5.27; N, 3.81. Found: C, 68.67, 68.84; H, 5.38, 5.41; N, 3.77, 3.78.

Example 104

[0221] To a solution of [(9,10-dioxo-9,10-dihydro-phenanthrene-3 -carbonyl)-amino]-acetic acid tert-butyl ester (200 mg, 550 μmol) in CH2Cl2 (10 mL) under N2 was added TFA (10 mL). This mixture was stirred for 2 h and concentrated under reduced pressure. The residue was dissolved in CH2Cl2 and evaporated once again to rid residual TFA. The material was recrystallized from refluxing EtOAc to afford [(9,10-dioxo-9,10-dihydro-phenanthrene-3-carbonyl)-amino]-acetic acid (70 mg, 47%). Orange solid; 1H NMR (300 MHz, DMSO-d6) δ12.7 (1H, s), 9.29 (1H, dd, J=5.7, 5.7 Hz), 8.71 (1H, s), 8.39 (1H, d, J=7.9 Hz), 8.13 (1H, d, J=8.3Hz), 8.07 (1H, dd, J=1.2, 7.5 Hz), 7.98 (1H, dd, J=1.6, 8.2Hz), 7.85 (1H, ddd, J=1.3, 8.7, 8.7 Hz), 7.59 (1H, dd, J=7.5, 7.5 Hz), 4.03 (2H, d, J=5.6 Hz); Anal. Calcd. For C17H11NO5-1.0H2O: C, 62.39; H, 4.00; N, 4.28. Found: C, 62.48, 62.36; H, 3.63, 3.61; N, 4.46, 4.47.

[0222] The compound of example 105 was prepared by the method of example 104, by utilizing [(9,10-dioxo-9,10-dihydro-phenanthrene-2-carbonyl)-amino]-acetic acid tert-butyl ester as the starting material.

Example 105:

[0223] [(9,10-Dioxo-9,10-dihydro-phenanthrene-2-carbonyl)-amino]-acetic acid:

[0224] Orange solid; 1H NMR (300 MHz, DMSO-d6) δ12.69 (1H, s), 9.22 (1H, dd, J=6.0, 6.0 Hz), 8.54 (1H, d, J=1.9 Hz), 8.46 (1H, d, J=8.2 Hz), 8.39 (1H, d, J=8.0 Hz), 8.23 (1H, dd, J=1.9, 8.4 Hz), 8.08 (1H, dd, J=1.1, 7.5Hz), 7.82(1H, ddd, J=1.5, 8.0, 8.0 Hz), 7.59 (1H, dd, J=8.0, 8.0 Hz), 3.97 (2H, d, J=5.8 Hz); Anal. Calcd. For C17H11NO5: C, 66.0; H, 3.58; N, 4.53. Found: C, 66.09, 66.28; H, 3.69, 3.71; N, 4.59, 4.59.

Example 106

[0225] 9,10-Phenanthrenedione was purchased from Aldrich Chemical Company, Milwaukee, Wis. and used as received.

[0226] Assays for Biological Activity

[0227] Method A:

[0228] Phosphatase assay using pNPP as substrate:

[0229] CD45 enzyme was obtained from BIOMOL (Plymouth Meeting, Pa.). Phosphatase activity was assayed in a buffer containing final concentrations of 25 mM imidazole at pH 7.0, 50 mM NaCl, 2.5 mM ethylenediaminetetraacetic acid (“EDTA”), 5 mM dithiothreitol (“DTT”) and 10 μg/mL bovine serum albumin (“BSA”) using pNPP as a substrate. Compounds were tested in a range from 30 to 0.01 μM, with a final concentration of 1 or 5% dimethylsulfoxide (“DMSO”), depending on the compound solubility. Activity was measured by following the increase in absorbance at 405 nm using a SpectraMax Plus spectrophotometric plate reader (Molecular Devices, Sunnyvale, Calif.).

[0230] Method B:

[0231] Cytotoxicity Assay:

[0232] Calcein-AM (Molecular Probes. Eugene, Oreg.) uptake, as a quantitative measure of cell viability, was used to evaluate the toxic effect of compounds on T cells. Briefly, PBMC were treated for 3-7 days with 3-10 μg/ml PHA, a potent T-cell mitogen, to preferentially expand the T-cell population. (Bradley, Linda M. Cell Poliferation in Selected Methods in Cellular Immunology, Eds. Mishell, B. B. and Shiigi, S. M., W. H. Freeman and Co., San Francisco, 1980.)

[0233] The T-cell lymphoblasts were purified by separation over Lymphoprep, plated at 2×105/well in a round bottom 96-well plate containing RPMI with compound and incubated overnight at 37° C. in an incubator containing 5% CO2. The dilution scheme and culture media were the same as those used in the T-cell proliferation assay. After the incubation period, cells were washed with Dulbecco's phosphate-buffered saline (D-PBS) and incubated with 1 μM Calcein-AM for 30-45 mim in D-PBS as described in the technical sheet provided with The LIVE/DEAD Viability/Cytotoxicity Kit from Molecular Probes. Percent viability was assessed on a fluorescent plate reader (excitation filter 485/20 nm; emission filter 530/25 nm) where the 100% control value is the fluorescence intensity observed in the absence of test compound.

[0234] Method C:

[0235] Phosphatase assay using lck 10-mer as substrate:

[0236] Phosphatase activity was assayed in 96 well plates in a buffer containing final concentrations of 25 mM HEPES at pH 7.2, 5 mM DTT and 10 μg/mL BSA using the lck carboxy-terminal peptide TEGQpYQPQP as the substrate (Cho, H., Krishnaraj, R., Itoh, M., Kitas, E., Bannwarth, W., Saito, H., Walsh, C. T. 1993. Substrate specificities of catalytic fragments of protein tyrosine phosphatases (HPTPb, LAR, and CD45) toward the phosphotyrosylpeptide substrates and thiophosphotyrosylated peptides as inhibitors. Protein Science 2:977-984). Compounds were tested in a range from 30 to 0.01 μM in a final concentration of 5% DMSO. Enzyme was incubated with substrate with or without compound, at room temperature for 1.5 h. At the end of the incubation period, BIOMOL “Green Reagent” (BIOMOL, Plymouth Meeting, Pa.) was added to each well, the plates incubated at room temperature for 30 min and absorbance read at 620 nm.

[0237] Method D:

[0238] Cell isolation and T cell proliferation assay:

[0239] Whole blood was obtained from healthy human blood donors. Peripheral blood mononuclear cells (“PBMC”) were isolated using Lymphoprep density-gradient centrifugation (Nycomed Amersham, Oslo, Norway), washed, counted and resuspended at 2×106 cells/mL in RPMI 1640 medium containing glutamine, 0.1 mg/mL gentamycin and 10% heat inactivated human serum. PBMC were transferred to 96-well plates (2×105 cells/well) containing compound or vehicle control, with the final concentration of DMSO not to exceed 0.3%, and incubated for 1 hour before addition of the activating anti-CD3 antibody, OKT3 (30ng/mL). After 24 hours in culture, the cells were pulsed with [3H]thymidine (1 μCi/well) overnight and harvested the next day onto 96-well Packard GF/C filter plates using a Packard Cell Harvester (Packard Instruments, Meriden, Conn.). The filter plate was dried, the bottom of the plate sealed, 25 μL of Microscint 20 scintillation fluid added to each well, the top of the plate sealed with TopSeal-A, and the plate counted on a Packard TopCount. The data from the TopCount is transferred into Excel 5 (Microsoft, Redmond, Wash.) and formatted for EC50 determination using Prism software (GraphPad Software, San Diego, Calif.).

[0240] Table 4 shows the inhibition of CD45 activity in the pNPP asssay and the lck assay certain compounds of the present invention. Inhibition in the T cell proliferation assay, as well as results from T cell cytotoxicity assay are shown.

4TABLE 4pNPP IC50Ick IC50T cell prolif.Example. No.(μM)(μM)IC50 (μM)CC50 (μM)10.6113.12020.5111.01430.7171.18.240.23.80.13.550.3121.08.260.5161.51270.5130.5980.4151.41290.85.20.89.1101.24.90.710112.811.66.5>30121.36.03.015131.15.81.5141412.9>300.85.3151.0156.4>30163.7>300.83.6170.8>301.87.3180.75>301.38.4194.49>301.64.4200.99>302.18.9210.99>301.84.9223.69.70.7>30231.13.020>30240.62.4>30>30251.22.5>30>30260.72.3>30>30270.73.1>30>30281.12.25>30291.12.520>30300.91.714.5>30310.62.57>30320.72.310.5>30331.12.516>30340.91.9>30ND350.91.4>30>30361.02.2>30>30371.12.1>30>30380.72.2>30ND390.72.3>30>30400.63.919.3>30411.43.58.8>30420.93.53.3>30431.93.89.0>30441.13.814.2>30451.43.95.0>30461.03.85.5>30470.74.23.0>30480.65.711>30490.96.116>30500.72.823>30511.14.814>30520.83.020>30531.03.716>30540.93.920>30551.44.7>30>30560.82.319>30571.33.221>30581.517.8>30ND591.15.8>30ND602.07.921.4ND611.88.523ND621.415.3>30ND630.72.61.28641.1>304.014651.13.10.615660.91.83.513671.02.20.84681.03.21.87.5690.64.84.614700.44.70.53710.53.71.31472>30NDNDND730.53.05.011740.83.32.619750.810.02.613769.3>307.0>30770.82.91.69780.76.91.328791.6>300.5>30805.94.90.76.5811.22.50.20.9820.52.91.17840.82.60.74851.42.80.5486>30NDNDND870.42.30.25883.7410.50.217890.82.90.29900.43.21.018910.44.70.59922.45.41.610930.53.91.310944.15.00.5895>30>30>3030960.373.10.65971.03.3>30>30981.02.4>30ND990.54.90.22.81000.84.20.83.51010.82.91.14.51020.63.40.32.51030.64.90.4101040.53.46.5>301050.82.24.4>301060.73.10.31.3

Cd45 inhibitors

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