1. Field of the Invention
The present invention relates to a cell colonies dissecting and transplanting apparatus that can use automatization equipment to precisely cut, capture and pickedly place the original cell colonies, so as to decrease the infection probability.
2. Description of the Prior Arts
Nowadays, the molecular biotechnology is generally applied in human disease researches, especially in the human embryonic stem cells studyings. Hence, mass-produce of said human embryonic stem cells for medical usages is an indispensable study in the molecular biotechnology industry.
A conventional culturing method of human embryonic stem cells as shown in
The present invention has arisen to mitigate and/or obviate the afore-described disadvantages.
The primary objective of the present invention is to provide a cell colonies dissecting and transplanting apparatus, wherein a movable holder for receiving a plurality of loading trays is disposed onto a base and can displace from a first axis feeding means and a second axis feeding means to moveably align with a first axis and a second axis; in addition, a cutting means and a transplanting means respectively capable of vertically move along two third axis feeding means are arranged onto the base, wherein the cutting means is provided with an array of invertable blades, and the transplanting means is defined with a bent capture needle for capturing the cell masses, whereby enabling to use the cutting means to dissect the original cell colonies into a plurality of cell masses, and then pickedly place said cell masses onto a plurality of media boards of subculture, while said cell colonies and the media boards of subculture are placed onto the loading trays of holder, so as to precisely cut, capture and pickedly place the cell colonies.
The secondary objective of the present invention is to provide a cell colonies dissecting and transplanting apparatus, wherein a platform having a CCD image capturing means therebottom is disposed onto the base, by means of the holder, the original cell colonies and the media boards of subculture can be moved onto the CCD image capturing means to capture their images, and then by using the operational analysis of controller, the cutting position of cutting means will be accurately modified to dissect the original cell colonies into a plurality cell masses, thereafter, said cell masses will be placed to the media boards of subculture such that the original cell colonies can be precisely cut, captured and pickedly placed.
The present invention will become more obvious from the following description when taken in connection with the accompanying drawings, which show, for purpose of illustrations only, a preferred embodiment in accordance with the present invention.
Referring to 18, a second axis (Y-axis) feeding means 12, a movable holder 13, two loading trays 14
15, a plurality of media boards 16
17, a cutting means 19, a transplanting means 20, two third axis feeding means 21
22, an auxiliary platform 23.
By using the first axis (X-axis) feeding means 11 and the second axis (Y-axis) feeding means 12, the holder 13 can be couplingly driven on the base 10, so as to move in X-axis and Y-axis directions. The two loading trays 1415 are respectively mounted onto the holder 13, wherein the loading tray 14 serves to receive the media boards 16 of cell colonies, while the loading tray 15 is applied to receive the media boards 17 of subculture. Moreover, by using the first axis (X-axis) feeding means 18, the cutting means 19 and the transplanting means 20 both can be couplingly driven on the base 10, so as to cause a movement above the two loading trays 14
15 in X-axis direction. The cutting means 19 and the transplanting means 20 can also be respectively driven by means of two third axis (Z-axis) feeding means 21
22, so as to cause a vertical movement above the two loading trays 14
15 in Z-axis direction. For the precision dissections of original cell colonies, the platform 23 having a CCD image capturing means therebottom is arranged onto the base 10, and then by using the holder 13, the media boards 16 of original cell colonies can be pickedly placed onto the CCD image capturing means to cause a CCD image capture, thereafter, by using operational analysis of controller, the cutting means 19 can be accurately moved onto the original cell colonies to cause a precision dissection.
Referring to
Referring to
While we have shown and described various embodiments in accordance with the present invention, it should be clear to those skilled in the art that further embodiments may be made without departing from the scope of the present invention.
Number | Date | Country | Kind |
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093141042 | Dec 2004 | TW | national |