Claims
- 1. An immortal ρ° cell line.
- 2. The immortal ρ° cell line of claim 1, wherein said cell line is a ρ° form of an immortal neural cell line.
- 3. The immortal ρ° cell line of claim 1 wherein said cell line is undifferentiated.
- 4. The undifferentiated immortal ρ° cell line of claim 3 wherein said cell line is capable of being induced to differentiate.
- 5. The immortal ρ° cell line of claim 4, wherein said cell line is a ρ° form of a neuroblastoma cell line.
- 6. The ρ° cell line of claim 5, wherein said cell line is a ρ° form of neuroblastoma cell line SH-SY5Y.
- 7. A cybrid cell line, comprising: the cultured immortal cells having genomic and mitochondrial DNAs of differing biological origins.
- 8. The cybrid cell line of claim 7, wherein said genomic DNA has its origin in an immortal ρ° cell line, and said mitochondrial DNA has its origin in a human tissue sample.
- 9. The cybrid cell line of claim 7, wherein said genomic DNA has its origin in an undifferentiated immortal ρ° cell line that is capable of being induced to differentiate, and said mitochondrial DNA has its origin in a human tissue sample.
- 10. The cybrid cell line of claim 9, wherein said undifferentiated immortal ρ° cell line is a ρ° form of a neuroblastoma cell line.
- 11. The cybrid cell line of claim 10, wherein said neuroblastoma cell line is derived from the neuroblastoma cell line SH-SY5Y.
- 12. The cybrid cell line of claim 8, wherein said human tissue sample is derived from a patient having a disease that is associated with mitochondrial defects.
- 13. The cybrid cell line of claim 9, wherein said human tissue sample is derived from a patient having a disease that is associated with mitochondrial defects.
- 14. The cybrid cell line of claim 9, wherein said undifferentiated immortal ρ° cell line is a ρ° form of a neuroblastoma cell line and said human tissue sample is derived from a patient having a neurological disease that is associated with mitochondrial defects.
- 15. The cybrid cell line of claim 8 wherein said human tissue sample is from a patient having a disorder selected from the group consisting of Alzheimer's Disease, Parkinson's Disease, Huntington's disease, dystonia, Leber's hereditary optic neuropathy, schizophrenia, myoclonic-epilepsy-lactic-acidosis-and-stroke (MELAS), and myoclonic-epilepsy-ragged-red-fiber—syndrome (MERRF).
- 16. The cybrid cell line of claim 9 wherein said undifferentiated immortal ρ° cell line is a ρ° form of neuroblastoma cell line SH-SY5Y and said human tissue sample is from a patient having a disorder selected from the group consisting of Alzheimer's disease, Parkinson's Disease, Huntington's disease, dystonia, Leber's hereditary optic neuropathy, schizophrenia, mitochondrial encephalopathy-lactic-acidosis -and-stroke (MELAS), and myoclonic-epilepsy-ragged-red-fiber—syndrome (MERRF).
- 17. The cybrid cell line of claim 9, wherein said undifferentiated immortal ρ° cell line is a ρ° form of neuroblastoma cell line SH-SY5Y and said human tissue sample is from a patient having Alzheimer's Disease.
- 18. A differentiated cybrid cell line resulting from induction of differentiation in cells of the cybrid cell line of claim 9.
- 19. A method of constructing a cybrid cell line, comprising the steps of:
a.) treating an immortal cell line with a chemical agent capable of irreversibly disabling mitochondrial electron transport and thus converting said cell line into an immortal ρ° cell line; and b.) transfecting said immortal ρ° cell line with isolated mitochondria to form said cybrid cell line.
- 20. The method of claim 19, wherein said immortal cell line is undifferentiated, but capable of being induced to differentiate.
- 21. The method of claim 19, wherein said isolated mitochondria are purified from a patient known to be afflicted with a disorder associated with a mitochondrial defect.
- 22. The method of claim 19, wherein said chemical agent is ethidium bromide.
- 23. A method of constructing cybrid cell lines, comprising the steps of:
a.) treating an immortal neuroblastoma cell line with ethidium bromide to irreversibly disable mitochondrial electron transport and thus convert said cell line into an immortal ρ° neuroblastoma cell line; and b.) transfecting said immortal ρ° neuroblastoma cell line with mitochondria isolated from tissue of a patient afflicted with a disorder selected from the group consisting of Alzheimer's Disease, Parkinson's Disease, Huntington's disease, dystonia, Leber's hereditary optic neuropathy, schizophrenia, myoclonic-epilepsy-lactic-acidosis -and-stroke (MELAS), and myoclonicepilepsy-ragged-red-fiber—syndrome (MERRF), to form said cybrid cell line.
- 24. A method for evaluating a compound for potential utility in the treatment of a disorder that is associated with mitochondrial defects, comprising the steps of:
a.) contacting a predetermined quantity of the test compound with cultured immortal cybrid cells having genomic DNA originating from an immortal ρ° cell line and mitochondrial DNA originating from tissue of a patient having a disease that is associated with mitochondrial defects; and b.) measuring a phenotypic trait in said cybrid cells that is affected by said mitochondrial defect; and c.) establishing whether and to what extent said drug is capable of causing said trait to become more similar to those of control cells having mitochondria that lack said defect, which capability indicates that the compound has potential utility in the treatment of said disorder.
- 25. A method for evaluating a compound for potential utility in the treatment of a disorder that is associated with mitochondrial defects according to claims 24, comprising the steps of:
a.) inducing the differentiation of cultured undifferentiated immortal cybrid cells having genomic DNA originating from an immortal ρ° cell line and mitochondrial DNA originating from tissue of a patient having a disease that is associated with mitochondrial defects; and b.) contacting a predetermined quantity of the test compound with said differentiated cybrid cells; and c.) measuring a phenotypic trait in said differentiated cybrid cells that is affected by said mitochondrial defect; and d.) establishing whether and to what extent said drug is capable of causing said trait to become more similar to those of control cells having mitochondria that lack said defect, which capability indicates that the compound has potential utility in the treatment of said disorder.
- 26. A method for the diagnosis of disorders that are associated with mitochondrial defects, comprising the steps of:
a.) obtaining from a patient a biological sample containing mitochondria; and b.) transferring said mitochondria into immortal ρ° cells to form cybrid cells; and c.) measuring a phenotypic trait in said cybrid cells that is caused by the mitochondrial defect associated with the disorder or disorders being tested for; and d.) establishing whether said cybrid cells exhibit said trait as do cells of patients suffering from said disorder, which indicates the presence of the disorder in said patient.
- 27. A method for the diagnosis of disorders that are associated with mitochondrial defects according to claim 26, comprising the steps of:
a.) obtaining from a patient a biological sample containing mitochondria; and b.) transferring said mitochondria into undifferentiated immortal ρ° cells to form cybrid cells; and c.) inducing said cybrid cells to differentiate; and d.) measuring one or more phenotypic trait in said differentiated cybrid cells that is caused by the mitochondrial defect associated with the disorder or disorders being tested for; and e.) establishing whether said cybrid cells exhibit said trait as do cells of patients suffering from said disorder, which indicates the presence of the disorder in said patient.
- 28. A cybrid animal comprising: a multicellular, non-human animal, having genomic and mitochondrial DNAs of differing biological origins.
- 29. A method of preparing a cybrid animal, comprising the steps of:
a.) isolating embryonic cells from a multicellular, non-human animal; and b.) treating said embryonic cells with a chemical agent capable of irreversibly disabling mitochondrial electron transport, thus converting said cells to a ρ° state; and c.) transfecting said immortal ρ° cell line with mitochondria isolated from another cell source, to produce said cybrid animal.
- 30. A method for evaluating a compound for potential utility in the treatment of a disorder that is associated with mitochondrial defects, comprising the steps of:
a.) contacting a predetermined quantity of the test compound with a cybrid animal of claim 28; and b.) measuring or observing one or more phenotypic trait in said cybrid animal that is affected by said mitochondrial defect; and c.) establishing whether and to what extent said drug is capable of causing said trait or traits to become more similar to those of control animals having mitochondria that lack said defect, which capability indicates that said compound has potential utility in the treatment of said disorder.
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. patent application Ser. No. 08/397,808, filed Mar. 3, 1995 (issued as U.S. Pat. No. 5,888,498), and is also a continuation of U.S. patent application Ser. No. 09/272,904, filed Mar. 19, 1999, which application is a continuation of U.S. patent application No. 08/397,808, filed Mar. 3, 1995 (issued as U.S. Pat. No. 5,888,498), which application is a continuation-in-part of U.S. patent application Ser. No. 08/219,842, filed Mar. 30, 1994 (issued as U.S. Pat. No. 5,565,323), and which applications are incorporated herein by reference in their entirety.
Continuations (2)
|
Number |
Date |
Country |
Parent |
09272904 |
Mar 1999 |
US |
Child |
09825525 |
Apr 2001 |
US |
Parent |
08397808 |
Mar 1995 |
US |
Child |
09272904 |
Mar 1999 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08219842 |
Mar 1994 |
US |
Child |
08397808 |
Mar 1995 |
US |