Cephalosporins

Abstract

Novel penicillins and cephalosporins and non-toxic salts thereof, which contain a mono- or di-oxo- or thioxo-piperazino(thio)carbonylamino group in molecule. These compounds are valuable antibacterial compounds for use in mammals including man. This disclosure relates to such compounds and a process for the preparation thereof.

Description

This invention relates to novel penicillins and cephalosporins and to a process for producing the same.

The compounds of the present invention have various characteristics including a broad antibacterial spectrum against Gram-positive and Gram-negative bacteria, and effective antibacterial activity particularly against Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus species. Furthermore, the compounds of the present invention possess high resistance to .beta.-lactamase produced from bacteria, and effective antibacterial activity even against clinical isolates of bacteria which are significant at present from the clinical standpoint. Accordingly, the compounds of the present invention are quite effective as therapeutic drugs for human and animal infectious diseases derived from the above-mentioned pathogenic microorganisms.

It has heretofore been known that 6-acylamino penicillanic acids and 7-acylaminocephalosporanic acids having an amino group at the .alpha.-position of the acyl group show strong antibacterial activity not only against Gram-positive bacteria but also against Gram-negative bacteria. However, there are the disadvantages that the known compounds described above show substantially no effective antibacterial activity against not only Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus species, which have been known as causes for clinically serious infectious diseases but also resistant bacteria which are frequently isolated at present from many clinical hospitals. And they tend to by hydrolyzed with .beta.-lactamase produced from many drug-resistant bacteria.

With an aim to obtain penicillins and cephalosporins having no disadvantages mentioned above, the present invention conducted extensive sutdies to find that novel compounds of formula (I) which appears hereinafter, which are prepared by bonding the moiety, ##STR1## wherein A, X, Y, R.sup.2, R.sup.3, n and m are as mentioned hereinafter, to the amino group in the acyl group of penicillins and cephalosporins, can sufficiently satisfy the above-mentioned aim and have extremely valuable therapeutic effects.

It is an object of this invention to provide novel penicillins and cephalosporins containing a mono- or di-oxo- or thioxo-piperazino(thio)carbonylamino group in molecule.

It is another object of this invention to provide novel penicillins and cephalosporins having a broad antibacterial spectrum.

It is a further object of the invention to provide novel penicillins and cephalosporins having high resistance to .beta.-lactamase produced from bacteria.

It is a still further object of the invention to provide novel penicillins and cephalosporins having effective antibacterial activity against clinical isolates of bacteria.

It is a still further object of the invention to provide a process for producing the novel penicillins and cephalosporins.

It is a still further object of the invention to provide a pharmaceutical composition containing the novel penicillins or cephalosporins as active ingredient.

Other objects and advantages of this invention will become apparent from the following description.

The compounds of the present invention are penicillins and cephalosporins represented by the general formula (I), ##STR2## wherein R represents an amino acid residue; R.sup.1 represents a hydrogen atom, a blocking group or a salt-forming cation; n represents 1 or 2; each of the n X's, which may be identical or different from one another, represents individually an oxygen or sulfur atom, and the n X's are attached in any combination to the 2-, 3- and/or 5-positions of the piperazine ring; m represents 4-n; each pair of R.sup.2 and R.sup.3 are linked to the same carbon atom, and m pairs of R.sup.2 and R.sup.3, which may be the same or different, represent individually a hydrogen atom, a halogen atom, a carboxyl group, or a substituted or unsubstituted alkyl, cycloalkyl, aryl, acyl, aralkyl, alkoxycarbonylalkyl, acyloxyalkyl, alkoxy, alkoxycarbonyl, cycloalkyloxycarbonyl, aralkoxycarbonyl, aryloxycarbonyl, amino or carbamoyl group; any pair of R.sup.2 and R.sup.3 together with a common carbon atom may form a cycloalkyl ring; A represents a hydrogen atom, a hydroxy group, a nitro group, a cyano group, or an unsubstituted or substituted alkyl, alkenyl, alkynyl, alkadienyl, cycloalkyl, cycloalkenyl, cycloalkadienyl, aryl, acyl, aralkyl, acyloxyalkyl, alkoxy, cycloalkyloxy, aryloxy, alkoxycarbonyl, cycloalkyloxycarbonyl, aryloxycarbonyl, aralkoxycarbonyl, alkylsulfonyl, cycloalkylsulfonyl, arylsulfonyl, carbamoyl, thiocarbamoyl, acylcarbamoyl, acylthiocarbamoyl, alkylsulfonylcarbamoyl, arylsulfonylcarbamoyl, alkylsulfonylthiocarbamoyl, arylsulfonylthiocarbamoyl, sulfamoyl, alkoxycarbonylthioalkyl, alkoxythiocarbonylthioalkyl, amino or heterocyclic group; Y represents an oxygen or sulfur atom; and <Z represents ##STR3## where R.sup.4 represents a hydrogen atom, a halogen atom, a hydroxy group, a cyano group, an azido group, a quaternary ammonium group, or an organic group linked through O, N or S.

In the above-mentioned general formula (I), R represents an amino acid residue. Examples of such amino acid residue include residues of amino acids derived from various aliphatic, araliphatic, aromatic, alicyclic and heterocyclic compounds, which amino acids may have the amino group at a position such as .alpha.-, .beta.- or .gamma.-position to the carboxyl group. Preferable as said R is an .alpha.-amino acid residue represented by the formula ##STR4## wherein R.sup.5 is an alkyl group such as methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl or the like; a cycloalkyl group such as cyclopentyl, cyclohexyl, cycloheptyl or the like; a cycloalkenyl group such as cyclopentenyl, cyclohexenyl, or the like; a cycloalkadienyl group such as cyclopentadienyl, cyclohexadienyl or the like; an aryl group such as phenyl, naphthyl or the like; an aralkyl group such as benzyl, phenetyl or the like; an aryloxy group such as phenoxy, naphthoxy or the like; an alkylthioalkyl group such as methylthiomethyl, ethylthiomethyl, methylthioethyl, ethylthioethyl or the like; or a heterocyclic group such as furyl, thienyl, oxazolyl, thiazolyl, isoxazolyl, isothiazolyl, imidazolyl, pyrazolyl, pyridyl, pyrazyl, pyrimidyl, pyridazyl, quinolyl, isoquinolyl, quinazolyl, indolyl, indazolyl, 1,3,4-oxadiazolyl, 1,2,4-oxadiazolyl, 1,3,4-thiadiazolyl, 1,2,4-thiadiazolyl or the like; each group represented by said R.sup.5 may be substituted by various groups, for example, halogen, hydroxy, nitro, alkyl, alkoxy, alkylthio, acyl, alkylsulfonylamino or the like; R.sup.6 represents a hydrogen atom; and R.sup.5 and R.sup.6 together with a common carbon atom may form a cycloalkyl ring such as cyclohexyl, cycloheptyl or the like; a cycloalkenyl ring such as cyclopentenyl, cyclohexenyl, or the like; or a cycloalkadienyl ring such as cyclopentadienyl, cyclohexadienyl, or the like.

In the general formula (I), R.sup.1 is a hydrogen atom, a blocking group or a salt-forming cation. The blocking group may be any of those which have heretofore been used in the field of penicillin or cephalosporin type compounds. Concretely, the blocking group includes (1) ester-forming groups capable of being removed by catalytic reduction, chemical reduction or hydrolysis under mild conditions e.g. arylsulfonylalkyl groups such as toluene-sulfonylethyl, etc.; substituted or unsubstituted aralkyl groups such as benzyl, 4-nitrobenzyl, diphenylmethyl, trityl, 3,5-di(tert.-butyl)-4-hydroxybenzyl, etc.; substituted or unsubstituted alkyl groups such as tert.-butyl, trichloroethyl, etc.; phenacyl group; alkoxyalkyl groups such as methoxymethyl, etc.; and unsubstituted or alkyl-substituted cyclic aminoalkyl groups such as piperidinoethyl, 4-methylpiperidinoethyl, morpholinoethyl, pyrrolidinoethyl, etc.; (2) ester-forming groups capable of being easily removed owing to enzymes in a living body, e.g. acyloxyalkyl groups such as pivaloyloxymethyl, etc.; phthalide group; and indanyl group; (3) silicon-containing groups, phosphorus-containing groups and tin-containing groups which are capable of being easily removed by treating with H.sub.2 O or an alcohol, such as (CH.sub.3).sub.3 Si--, ##STR5## (C.sub.4 H.sub.9).sub.3 Sn--, or the like. The examples of the blocking groups mentioned in above (1), (2) and (3) are merely typical, and other examples are disclosed in U.S. Pat. Nos. 3,499,909; 3,573,296 and 3,641,018 and DOS 2,301,014; 2,253,287 and 2,337,105 and may be used in this invention. The salt-forming cation includes cations which have heretofore been known in the field of penicillin or cephalosporin type compounds, and preferable are those capable of forming non-toxic salts. The salts include alkali metal salts such as sodium salt, potassium salt, etc.; alkaline earth metal salts such as calcium salt, magnesium salt, etc.; ammonium salt; and salts with nitrogen-containing organic bases such as procaine, dibenzylamine, N-benzyl-.beta.-phenethylamine, 1-ephenamine, N,N-dibenzylethylenediamine, etc. In addition to the above cations, there may be used cations capable of forming the salts with other nitrogen-containing organic bases, such as trimethylamine, triethylamine, tributylamine, pyridine, dimethylaniline, N-methylpiperidine, N-methylmorpholine, diethylamine, dicyclohexylamine, etc. Furthermore, the cation includes quaternary ammonium groups formed at the 3-position of cephem ring, such as pyridinium, quinolinium, isoquinolinium, pyrimidinium, and the like. In this case, a betaine structure is formed in the molecule.

In the general formula (I), m pairs of R.sup.2 and R.sup.3, which may be the same or different, represent individually, a hydrogen atom; a halogen atom such as fluorine, chlorine, bromine, etc.; a carboxyl group; an alkyl group such as methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, etc.; a cycloalkyl group such as cyclopentyl, cyclohexyl, cycloheptyl, etc.; an aryl group such as phenyl, naphthyl, etc.; an acyl group such as acetyl, propionyl, butyryl, benzoyl, etc.; an aralkyl group such as benzyl, phenethyl, etc.; an alkoxycarbonylalkyl group such as methoxycarbonylmethyl, ethoxycarbonylmethyl, etc.; an acyloxyalkyl group such as acetyloxymethyl, propionyloxymethyl, pivaloyloxymethyl, benzoyloxymethyl, etc.; an alkoxy group such as methoxy, ethoxy, propoxy, butoxy, etc.; an alkoxycarbonyl group such as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, etc.; a cycloalkyloxycarbonyl group such as cyclopentyloxycarbonyl, cyclohexyloxycarbonyl, cycloheptyloxycarbonyl, etc.; an aralkoxycarbonyl group such as benzyloxycarbonyl, phenethoxycarbonyl, etc.; an aryloxycarbonyl group such as phenoxycarbonyl, naphthoxycarbonyl, etc.; an amino group such as amino, N-alkylamino (e.g. N-methylamino, N-ethylamino, N-propylamino, N-butylamino, etc.), N,N-dialkylamino (e.g. N,N-dimethylamino, N,N-diethylamino, N,N-dibutylamino, etc.), N-acylamino (e.g. N-acetylamino, N-propionylamino, N-butyrylamino, N-benzoylamino, etc.), and cyclic amino (e.g. pyrrolidino, piperidino, morpholino, etc.); and a carbamoyl group such as carbamoyl, N-methylaminocarbonyl, N-ethylaminocarbonyl, N,N-dimethylaminocarbonyl, N,N-diethylaminocarbonyl, etc. Further, R.sup.2 and R.sup.3 together with a common carbon atom may form a cycloalkyl ring such as a cyclopentyl, cyclohexyl or cycloheptyl group. Each of the groups mentioned above for said R.sup.2 and R.sup.3 may be substituted by various substituents, for example, halogen atoms, or alkyl, alkoxy, alkylthio, acyl or nitro groups.

In the general formula (I), A represents a hydrogen atom; a hydroxy group; a nitro group; a cyano group; an alkyl group such as methyl, ethyl, propyl, isopropyl, butyl, pentyl, hexyl, heptyl, octyl, dodecyl, or the like; an alkenyl group such as vinyl, propenyl, butenyl, or the like; an alkynyl group such as propargyl, or the like; an alkadienyl group such as 1,3-butadienyl, 1,3-pentadienyl, or the like; a cycloalkyl group such as cyclopentyl, cyclohexyl, cycloheptyl, or the like; a cycloalkenyl group such as cyclopentenyl, cyclohexenyl, or the like; a cycloalkadienyl group such as cyclopentadienyl, cyclohexadienyl, or the like; an aryl group such as phenyl, naphthyl, or the like; an acyl group such as formyl, acetyl, propionyl, isovaleryl, caproyl, enanthoyl, capryloyl, palmitoyl, stearoyl, acryloyl, cyclohexanecarbonyl, benzoyl, phenylglycyl, furoyl, thenoyl, or the like; an aralkyl group such as benzyl, phenethyl, or the like; an acyloxyalkyl group such as acetyloxyethyl, pivaloyloxymethyl, benzoyloxymethyl, or the like; an alkoxy group such as methoxy, ethoxy, propoxy, butoxy, or the like; a cycloalkyloxy group such as cyclopentyloxy, cyclohexyloxy, cycloheptyloxy, or the like; an aryloxy group such as phenoxy, naphthoxy, or the like; an alkoxycarbonyl group such as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, butoxycarbonyl, or the like; a cycloalkyloxycarbonyl group such as cyclopentyloxycarbonyl, cyclohexyloxycarbonyl, cycloheptyloxycarbonyl, or the like; an aryloxycarbonyl group such as phenoxycarbonyl, (1- or 2-)naphthoxycarbonyl, or the like; an aralkoxycarbonyl group such as benzyloxycarbonyl, phenethoxycarbonyl, or the like; an alkylsulfonyl group such as methanesulfonyl, ethanesulfonyl, propanesulfonyl, butanesulfonyl, or the like; a cycloalkylsulfonyl group such as cyclopentanesulfonyl, cyclohexanesulfonyl, or the like; an arylsulfonyl group such as benzenesulfonyl, (1- or 2-)naphthalenesulfonyl, or the like; a carbamoyl group such as carbamoyl, N-alkylaminocarbonyl (e.g. N-methylaminocarbonyl, N-ethylaminocarbonyl, N-propylaminocarbonyl, N-butylaminocarbonyl, or the like), N-arylaminocarbonyl (e.g. N-phenylaminocarbonyl, or the like), N,N-dialkylaminocarbonyl (e.g. N,N-dimethylaminocarbonyl, N,N-diethylaminocarbonyl, or the like), cyclic amino carbonyl (e.g. pyrrolidinocarbonyl, piperidinocarbonyl, morpholinocarbonyl, or the like); a thiocarbamoyl group such as thiocarbamoyl, N-alkylaminothiocarbonyl (e.g. N-methylaminothiocarbonyl, N-ethylaminothiocarbonyl, N-propylaminothiocarbonyl, or the like), N-arylaminothiocarbonyl (e.g. N-phenylaminothiocarbonyl, or the like), N,N-dialkylaminothiocarbonyl (e.g. N,N-dimethylaminothiocarbonyl, N,N-diethylaminothiocarbonyl, or the like), or cyclic aminothiocarbonyl (e.g. pyrrolidinothiocarbonyl, piperidinothiocarbonyl, morpholinothiocarbonyl, or the like); an acylcarbamoyl group such as N-acetylcarbamoyl, N-propionylcarbamoyl, N-butyrylcarbamoyl, N-benzoylcarbamoyl, N-furoylcarbamoyl, N-thenoylcarbamoyl, or the like; an acylthiocarbamoyl group such as N-acetylthiocarbamoyl, N-propionylthiocarbamoyl, N-butyrylthiocarbamoyl, N-benzoylthiocarbamoyl, N-naphthoylthiocarbamoyl, N-furoylthiocarbamoyl, N-thenoylthiocarbamoyl, or the like); an alkylsulfonylcarbamoyl group such as methanesulfonylaminocarbonyl, ethanesulfonylaminocarbonyl, butanesulfonylaminocarbonyl, or the like; an arylsulfonylcarbamoyl group such as benzenesulfonylaminocarbonyl, (1- or 2-)naphthalenesulfonylaminocarbonyl, or the like; an alkylsulfonylthiocarbamoyl group such as methanesulfonylaminothiocarbonyl, ethanesulfonylaminothiocarbonyl butanesulfonylaminothiocarbonyl, or the like; an arylsulfonylthiocarbamoyl group such as benzenesulfonylaminothiocarbonyl, naphthalenesulfonylaminothiocarbonyl, or the like; a sulfamoyl group such as sulfamoyl, N-methylsulfamoyl, N-ethylsulfamoyl, N-propylsulfamoyl, N-butylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N,N-dipropylsulfamoyl, N,N-dibutylsulfamoyl, N-phenylsulfamoyl, N-benzylsulfamoyl, N-cyclopentylsulfamoyl, N-cyclohexylsulfamoyl, or the like; an alkoxycarbonylthioalkyl group such as methoxycarbonylthiomethyl, ethoxycarbonylthiomethyl, propoxycarbonylthiomethyl, butoxycarbonylthiomethyl, methoxycarbonylthioethyl, or the like; an alkoxythiocarbonylthioalkyl group such as methoxythiocarbonylthiomethyl, ethoxythiocarbonylthiomethyl, propoxythiocarbonylthiomethyl, butoxythiocarbonylthiomethyl, methoxythiocarbonylthioethyl, or the like; an amino group such as amino, N-alkylamino (e.g. N-methylamino, N-ethylamino, N-propylamino, N-butylamino, or the like), N,N-dialkylamino (e.g. N,N-dimethylamino, N,N-diethylamino, N,N-dibutylamino, or the like), N-acylamino (e.g. N-acetylamino, N-propionylamino, N-butyrylamino, N-benzoylamino, or the like), or cyclic amino (e.g. pyrrolidino, piperidino, morpholino, or the like); or a heterocyclic group such as thiazolyl, pyridyl, pyridazyl, pyrazyl, thiadiazolyl, triazolyl, tetrazolyl, quinolyl, or the like, Each of the groups mentioned above for A in formula (I) may be substituted by any of such substitutents as, for example, halogen atoms, hydroxyl groups, alkyl groups, alkoxy groups, alkylthio groups, nitro groups, cyano groups, amino groups (e.g. dialkylamino, cyclic amino, etc.), carboxyl groups, acyl groups, etc.

The quaternary ammonium groups for R.sup.4 include pyridinium, quinolinium, isoquinolinium and pyrimidinium. Further, the organic group which is linked through O, N or S for R.sup.4 includes alkoxy groups such as methoxy, ethoxy, propoxy, etc.; aryloxy groups such as phenoxy, naphthoxy, etc.; aralkoxy groups such as benzyloxy, phenethoxy, etc.; acyloxy groups such as acetyloxy; propionyloxy, butyryloxy, benzoyloxy, naphthoyloxy, cyclopentanecarbonyloxy, cyclohexanecarbonyloxy, furoyloxy, thenoyloxy, etc.; carbamoyloxy groups such as carbamoyloxy, N-methylaminocarbonyloxy, N,N-dimethylaminocarbonyloxy, N-acetylaminocarbonyloxy, phenylaminocarbonyloxy, benzylaminocarbonyloxy, cyclohexylaminocarbonyloxy, etc.; guanidino groups such as guanidino, N-methylguanidino, etc.; amino groups such as amino, N-alkylamino (e.g. N-methylamino, N-ethylamino, N-propylamino, N-butylamino, N-cyclohexylamino, N-phenylamino, etc.), N,N-dialkylamino (e.g. N,N-dimethylamino, N,N-diethylamino, N,N-dibutylamino, etc.), and cyclic amino (e.g. pyrrolidino, piperidino, morpholino, etc.); alkylthio groups such as methylthio, ethylthio, propylthio, etc.; arylthio groups such as phenylthio, (1- or 2-)-naphthylthio, etc.; aralkylthio groups such as benzylthio, phenethylthio, etc.; acylthio groups such as acetylthio, propionylthio, butyrylthio, benzoylthio, (1- or 2-)naphthoylthio, cyclopentanecarbonylthio, cyclohexanecarbonylthio, furoylthio, thenoylthio, isothiazolecarbonylthio, isoxazolecarbonylthio, thiadiazolecarbonylthio, triazolecarbonylthio, etc.; thiocarbamoylthio groups such as thiocarbamoylthio, N-methyl-thiocarbamoylthio, N,N-diethylthiocarbamoylthio, 1-piperidino-thiocarbonylthio, 1morpholino-thiocarbonylthio, 4-methyl-1-piperazinothiocarbonylthio, etc.; alkoxythiocarbonylthio groups such as methoxythiocarbonylthio, ethoxythiocarbonylthio, propoxythiocarbonylthio, butoxythiocarbonylthio, etc.; aryloxythiocarbonylthio groups such as phenoxythiocarbonylthio, etc.; cycloalkyloxythiocarbonylthio groups such as cyclohexyloxythiocarbonylthio, etc.; amidinothio groups such as amidinothio, N-methylamidinothio, N,N'-dimethylamidinothio, etc.; and heterocycle thio groups such as oxazolylthio, thiazolylthio, isoxazolylthio, isothiazolylthio, imidazolylthio, pyrazolylthio, pyridylthio, pyrazinylthio, pyrimidinylthio, pyridazinylthio, quinolylthio, isoquinolylthio, quinazolylthio, indolylthio, indazolylthio, oxadiazolylthio, thiadiazolylthio, triazolylthio, tetrazolylthio, triazinylthio, benzimidazolylthio, benzoxazolylthio, benzothiazolylthio, triazolopyridylthio, purinylthio, pyridine-1-oxide-2-ylthio, pyridazine-1-oxide-6-ylthio, etc. Each of the groups mentioned above for R.sup.4 may be substituted by any of such substituents as, for example, halogen atoms, alkyl groups, alkoxy groups, alkylthio groups, nitro groups, cyano groups, acylamino groups, acyl groups, carboxyl groups, carbamoyl groups, etc.

By the term "aryl" and its root "ar" applicants intend univalent aromatic hydrocarbon radicals having the free valence at ring atom. By the term "acyl" applicants intend the carboxylic acyl radical. By the term "cyclic amino" is meant a saturated heterocyclic amine whose free valence is attached to a ring amino nitrogen.

The above-mentioned compounds of formula (I) of the present invention have their optical isomers, and all of D-isomers, L-isomers and racemic compounds thereof are involved in the scope of the present invention.

In the present invention, preferable compounds of the general formula (I) are as follows: ##STR6## wherein R.sup.1, R.sup.2, R.sup.3, A, R.sup.5 and <Z are as defined above.

The compounds of formula (I) of the present invention are produced according to either the process (1), (2) or (3) described below.

Process (1):

A process comprising reacting a compound represented by the general formula (II), ##STR7## with a reactive derivative in the ##STR8## group (hereinafter referred to as "(thio)carboxyl group") of a compound represented by the general formula (III), ##STR9##

Process (2):

A process comprising reacting a compound represented by the general formula (IV), ##STR10## with a compound represented by the general formula (V), ##STR11## or with a reactive derivative in the ##STR12## group (hereinafter referred to as "carboxyl group") of the compound of formula (V).

Process (3):

A process comprising reacting a compound represented by the general formula (VI), ##STR13## with a compound represented by the general formula (VII),

R.sup.8 M (VII) or with a tertiary amine.

In the above-mentioned formulas (II) to (VI), R, R.sup.1, R.sup.2, R.sup.3, R.sup.4, X, m, n, A, Y and <Z are as defined above; and R.sup.7 represents a hydrogen atom, a silicon-containing group or a phosphorus-containing group, these silicon-containing and phosphorus-containing groups having the same meanings as mentioned above for R.sup.1.

In the aforesaid formula (VI), B represents a substituent capable of being easily replaced by a nucleophilic reagent, and includes, for example, halogen atoms such as chlorine, bromine, etc.; lower alkanoyloxy groups such as formyloxy, acetoy, propionyloxy, butyryloxy, pivaloyloxy, etc.; arylcarbonyloxy groups such as benzoyloxy, naphthoyloxy, etc.; arylcarbonylthio groups such as benzoylthio, naphthoylthio, etc.; carbamoyloxy groups; heteroaromatic amine N-oxide thio groups having a thio group on the carbon atom adjacent to the N-oxide group in the molecule, such as pyridine-1-oxide-2-ylthio, pyridazine-1-oxide-6-ylthio, etc. Each of the groups mentioned above for B may be substituted by any of such substituents as, for example, halogen atoms, nitro groups alkyl groups, alkoxy groups, alkylthio groups, acyl groups, etc.

In formula (VIII), R.sup.8 represents a cyano group, an azido group or an organic group linked through O, N or S, and this organic group is the same as mentioned above for R.sup.4.

In the formula (VII), M represents a hydrogen atom, an alkali metal or an alkaline earth metal. The tertiary amine used in the process (3) includes pyridine, quinoline, isoquinoline, pyrimidine, etc. These tertiary amines may be substituted by various substituents such as halogen, lower alkyl, carbamoyl and the like.

As the compound (II), there may be used any of D-isomers, L-isomer or racemic compound.

As the reactive derivative of the (thio)carboxyl group of the compound of formula (III), there is used a reactive derivative of a carboxylic acid which is ordinarily employed for the synthesis of acid amide compounds. Examples of the reactive derivative are acid halides, acid azides, acid cyanides, mixed acid anhydrides, active esters, active amides, etc. Particularly preferable examples thereof are acid halides such as acid chlorides, acid bromies, etc., and active esters such as cyanomethyl ester, trichloromethyl ester, etc.

The reactive derivative of the (thio)carboxyl group of the compound of formula (III) can be easily obtained by reacting, for example, an oxopiperazine or thioxopiperazine of formula (VIII) synthesized according to the process of the literature references described below, with phosgene, thiophosgene, trichloromethyl ester of chloroformic acid, or the like, ##STR14## wherein A, X, R.sup.2, R.sup.3, m and n are as defined previously. Literature references:

V. G. Granik, Khim-Farm. Zh., I(4), 16-19 (1967) (Russ); Samuel R. Aspinall, J. An. Chem. Soc., 62, 1202-4 (1940); Kuniyoshi MASUZAWA, Pharm. Bull. (Japan), 38 2078-2081 (1966); Arthur P. Phillips, Ger. 1135472, Aug. 30 (1962); J. L. Riebsomer, J. Org. Chem., 15 68-73 (1950); Jongkees, Rec. trav. Chim., 27 305; Patric T. Izzo, J. Am. Chem. Soc., 81 4668-4670 (1959); and B. H. Chase & A. M. Downes, J. Chem. Soc., 3874-3877 (1953).

Concrete examples of the compound of formula (VIII) and the reactive derivative of (thio)carboxyl group of the compound of formula (III) are as set forth in Table 1 and Table 2, respectively, but it is needless to say that these are not limitative.

TABLE 1__________________________________________________________________________ ##STR15## (VIII) m.p. (recrystallization IRCompound solvent) (cm.sup.-1)__________________________________________________________________________ ##STR16## ##STR17## .nu..sub.C.dbd.O 1640 .nu..sub.NH 3450-3250 ##STR18## b.p. 143.degree. C./1 mmHg oily material .nu..sub.C.dbd.O 1650 .nu..sub.NH 3300-3200 ##STR19## b.p. 122-125.degree. C./2 mmHg 140-141.degree. C. (IPA) .nu..sub.C.dbd.O 1650-1630 .nu..sub.NH 3260, 3170 ##STR20## 85-86.degree. C. (IPAIPE) .nu..sub.C.dbd.O 1660-1620 ##STR21## 105-106.degree. C. (AcOEt) .nu..sub.C.dbd.O 1710, 1640 .nu..sub.NH 3300, 3190 ##STR22## ##STR23## .nu..sub.C.dbd.O 1645, 1625 .nu..sub.NH 3380, 3220 ##STR24## 129-130.degree. C. (IPA) .nu..sub.C.dbd.O 1650, 1630 .nu..sub.NH 3270 ##STR25## 134-135.degree. C. (IPA) .nu..sub.C.dbd.O 1660-1630 .nu..sub.NH 3280 ##STR26## 96-97.degree. C. (CCl.sub.4) .nu..sub.C.dbd.O 1670, 1640 .nu..sub.NH 3200 ##STR27## 80-81.degree. C. (IPE) .nu..sub.C.dbd.O 1660, 1620 .nu..sub.NH 3250 ##STR28## 83-84.degree. C. (IPE) .nu..sub.C.dbd.O 1660, 1620 .nu..sub.NH 3250 ##STR29## 99-100.degree. C. (CCl.sub.4) .nu..sub.C.dbd.O 1660, 1620 .nu..sub.NH 3250 ##STR30## 203-205.degree. C. (IPA) .nu..sub.C.dbd.O 1670, 1620 .nu..sub.NH 3250 ##STR31## 91-93.degree. C. (IPA) .nu..sub.C.dbd.O 1640, 1600 .nu..sub.NH 3250 ##STR32## 146-148.degree. C. (IPA) .nu..sub.C.dbd.O 1650, 1620 .nu..sub.NH 3200 ##STR33## 118-120.degree. C. (IPA) .nu..sub.C.dbd.O 1660, 1620 .nu..sub.NH 3200 ##STR34## 182-185.degree. C. (IPA) .nu..sub.C.dbd.O 1670, 1600 .nu..sub.NH 3200 ##STR35## Oily material .nu..sub.C.dbd.O 1650, 1620 .nu..sub.NH 3200 ##STR36## ##STR37## .nu..sub.C.dbd.O 1650, 1630 .nu..sub.NH 3225 ##STR38## 167-168.degree. C. (EtOH) .nu..sub.C.dbd.O 1680 .nu..sub.NH 3200 .nu..sub.SO.sbsb.2.sub.N< 1310, 1140 ##STR39## ##STR40## .nu..sub.C.dbd.O 1680, 1650 1620 .nu..sub.NH 3300 ##STR41## 85-88.degree. C. (AcOEt) .nu..sub.C.dbd.O 1660, 1640 .nu..sub.NH 3300, 3200 ##STR42## ##STR43## .nu..sub.C.dbd.O 1690-1650 .nu..sub.NH 3200, 3050 ##STR44## 189-190.degree. C. (IPA) .nu..sub.C.dbd.O 1650, 1620 .nu..sub.NH 3250 ##STR45## 136-138.degree. C. (Acetone) .nu..sub.C.dbd.O 1660 .nu..sub.NH 3200 ##STR46## Oily material .nu..sub.C.dbd.O 1650-1630 .nu..sub.NH 3270 ##STR47## Oily material .nu..sub.NH 3250 .nu..sub.C.dbd.O 1650-1630 ##STR48## Oily material .nu..sub.C.dbd.O 1650-1620 ##STR49## Oily material .nu..sub.NH 3270 .nu..sub.C.dbd.O 1650-1630 Hydrochloride .nu..sub.C.dbd.O 1680 .nu..sub.NH 3200, 3080 ##STR50## Oily material .nu..sub.C.dbd.O 1680 .nu..sub.NH 3300 ##STR51## Oily material .nu..sub.C.dbd.O 1720, 1640 .nu..sub.NH 3300 ##STR52## b.p. 104.degree. C./4 mmHg .nu..sub.C.dbd.O 1620 .nu..sub.NH 3275 ##STR53## Oily material .nu..sub.C.dbd.O 1610 .nu..sub.NH 3250 ##STR54## Oily material .nu..sub.C.dbd.O 1610 .nu..sub.NH 3250 ##STR55## Oily material .nu..sub.C.dbd.O 1610 .nu..sub.NH 3400-3200 ##STR56## Oily material .nu..sub.C.dbd.O 1620 .nu..sub.NH 3270 ##STR57## Oily material .nu..sub.C.dbd.O 1620 .nu..sub.NH 3270 ##STR58## Oily material .nu..sub.C.dbd.O 1620 .nu..sub.NH 3270 ##STR59## Oily material .nu..sub.C.dbd.O 1620 .nu..sub.NH 3270 ##STR60## Oily material .nu..sub.C.dbd.O 1620 .nu..sub.NH 3270 ##STR61## Oily material .nu..sub.C.dbd.O 1620 .nu..sub.NH 3270 ##STR62## Oily material .nu..sub.C.dbd.O 1620 .nu..sub.NH 3300 ##STR63## Oily material .nu..sub.C.dbd.O 1630 .nu..sub.NH 3300 ##STR64## Oily material .nu..sub.C.dbd.O 1630 .nu..sub.NH 3300 ##STR65## Oily material .nu..sub.C.dbd.O 1630 .nu..sub.NH 3200 ##STR66## ##STR67## .nu..sub.C.dbd.O 1630 .nu..sub.NH 3300 ##STR68## Oily material .nu..sub.C.dbd.O 1700 .nu..sub.NH 3400-3250 ##STR69## b.p. 183-185.degree. C./2 mmHg .nu..sub.C.dbd.O 1620 ##STR70## Oily material .nu..sub.C.dbd.O 1650 .nu..sub.NH 3300 ##STR71## Oily material .nu..sub.C.dbd.O 1620 .nu..sub.NH 3300 ##STR72## Oily material .nu..sub.C.dbd.O 1640 .nu..sub.NH 3300 ##STR73## Oily material .nu..sub.C.dbd.O 1660 .nu..sub.NH 3350 ##STR74## Oily material .nu..sub.C.dbd.O 1630 .nu..sub.NH 3300 ##STR75## 184-185.degree. C. (EtOH) .nu..sub.C.dbd.O 1690-1650 .nu..sub.NH 3190, 3050 ##STR76## 177-178.degree. C. (EtOH) .nu..sub.C.dbd.O 1680-1650 .nu..sub.NH 3190, 3050 ##STR77## 142-143.degree. C. (IPA) .nu..sub.C.dbd.O 1680-1620 .nu..sub.NH 3200 ##STR78## 209.degree. C. (IPA) .nu..sub.C.dbd.O 1660-1630 .nu..sub.NH 3230 ##STR79## 158.degree. C. (IPA) .nu..sub.C.dbd.O 1695, 1660 .nu..sub.NH 3220 ##STR80## Oily material .nu..sub.C.dbd.O 1730-1650 .nu..sub.NH 3300-3200 ##STR81## ##STR82## .nu..sub.C.dbd.O 1680, 1650 .nu..sub.NH 3250 ##STR83## ##STR84## .nu..sub.C.dbd.O 1680, 1650 .nu..sub.NH 3200, 3100 ##STR85## 111-113.degree. C. (CCl.sub.4) .nu..sub.C.dbd.O 1695, 1670 .nu..sub.NH 3240, 3150 ##STR86## ##STR87## .nu..sub.C.dbd.O 1650 .nu..sub.NH 3300-3200 ##STR88## 104-106.degree. C. (IPE) .nu..sub.C.dbd.O 1700, 1660 .nu..sub.NH 3200, 3100 ##STR89## 111-115.degree. C. (IPE) .nu..sub.C.dbd.O 1700, 1660 .nu..sub.NH 3200, 3100 ##STR90## 112-115.degree. C. (IPE) .nu..sub.C.dbd.O 1700, 1660 .nu..sub.NH 3200, 3100 ##STR91## 116-120.degree. C. (IPE) .nu..sub.C.dbd.O 1700, 1660 .nu..sub.NH 3225, 3100 ##STR92## 136-137.degree. C. (Acetone) .nu..sub.C.dbd.O 1680, 1655 .nu..sub.NH 3200, 3100 ##STR93## 202-204.degree. C. (IPA) .nu..sub.C.dbd.O 1690, 1645 .nu..sub.NH 3260 ##STR94## 128-129.degree. C. (EtOH) .nu..sub.C.dbd.O 1700-1650 .nu..sub.NH 3200-3100 ##STR95## 127-128.degree. C. (AcOEt) .nu..sub.C.dbd.O 1660 .nu..sub.NH 3200, 3080 ##STR96## ##STR97## .nu..sub.C.dbd.O 1660 .nu..sub.NH 3200, 3100 ##STR98## 183-185.degree. C. (EtOH) .nu..sub.C.dbd.O 1720, 1660 .nu..sub.NH 3220, 3175, 3050 ##STR99## 96-99.degree. C. (IPAn-Hexane) .nu. .sub.C.dbd.O 1720, 1660 .nu..sub.NH 3330 ##STR100## 143-146.degree. C. (IPA) .nu..sub.C.dbd.O 1765, 1720, 1680 .nu..sub.NH 3350 ##STR101## 210-212.degree. C. (MeOH) .nu..sub.C.dbd.O 1680 .nu..sub.NH 3380, 3290, 3070 ##STR102## 132-133.degree. C. (EtOH) .nu..sub.C.dbd.O 1715, 1685 .nu..sub.NH 3275, 3170 ##STR103## 98-100.degree. C. (IPA) .nu..sub.C.dbd.O 1715, 1665 .nu..sub.NH 3360__________________________________________________________________________ Note: IPA = (CH.sub.3).sub.2 CHOH IPE = (CH.sub.3).sub.2 CHOCH(CH.sub.3).sub.2 AcOEt = CH.sub.3 COOCH.sub.2 CH.sub.3 EtOH = CH.sub.3 CH.sub.2 OH TABLE 2__________________________________________________________________________ ##STR104## (III)Compound Physical property I.R. (cm.sup.-1)__________________________________________________________________________ ##STR105## Oily material .nu..sub.C.dbd.O 1790, 1710, 1640 ##STR106## Oily material .nu..sub.C.dbd.O 1790, 1730-1650 ##STR107## Oily material .nu..sub.C.dbd.O 1790, 1730-1650 ##STR108## Oily material .nu..sub.C.dbd.O 1740, 1660, 1640 ##STR109## Oily material .nu..sub.C.dbd.O 1740, 1680-1640 ##STR110## Oily material .nu..sub.C.dbd.O 1740, 1680-1640 ##STR111## Oily material .nu..sub.C.dbd.O 1790, 1710, 1640 ##STR112## Oily material .nu..sub.C.dbd.O 1790, 1730, 1640 ##STR113## Oily material .nu..sub.C.dbd.O 1740, 1660, 1630 ##STR114## Oily material .nu..sub.C.dbd.O 1740, 1640 ##STR115## Oily material .nu..sub.C.dbd.O 1730, 1650 ##STR116## Oily material .nu..sub.C.dbd.O 1740, 1640 ##STR117## Oily material .nu..sub.C.dbd.O 1720, 1640 ##STR118## Oily material .nu..sub.C.dbd.O 1790, 1710, 1640 ##STR119## Oily material .nu..sub.C.dbd.O 1790, 1700 .nu..sub.SO.sbsb.2 1320, 1140 ##STR120## Oily material .nu..sub.C.dbd.O 1790, 1720-1660 ##STR121## Oily material .nu..sub.C.dbd.O 1740, 1720, 1650 ##STR122## Oily material .nu..sub.C.dbd.O 1750, 1720, 1640 ##STR123## Oily material .nu..sub.C.dbd.O 1740-1720, 1670 ##STR124## Oily material .nu..sub.C.dbd.O 1790, 1720 ##STR125## Oily material .nu..sub.C.dbd.O 1790, 1720 ##STR126## Oily material .nu..sub.C.dbd.O 1790, 1720 ##STR127## Oily material .nu..sub.C.dbd.O 1790, 1720 ##STR128## ##STR129## .nu..sub.C.dbd.O 1720, 1660 ##STR130## Crystal .nu..sub.C.dbd.O 1730, 1670 ##STR131## Crystal .nu..sub.C.dbd.O 1720, 1660 ##STR132## m.p. 59-60.degree. C. (from IPE) .nu..sub.C.dbd.O 1710-1730, 1660 ##STR133## ##STR134## .nu..sub.C.dbd.O 1725, 1650 ##STR135## Oily material .nu..sub.C.dbd.O 1720, 1690 ##STR136## Oily material .nu..sub.C.dbd.O 1790, 1740-1700 ##STR137## Oily material .nu..sub.C.dbd.O 1710, 1630 ##STR138## Oily material .nu..sub.C.dbd.O 1730, 1650 ##STR139## Oily material .nu..sub.C.dbd.O 1730, 1650 ##STR140## Oily material .nu..sub.C.dbd.O 1720, 1640 ##STR141## Oily material .nu..sub.C.dbd.O 1730, 1640 ##STR142## Oily material .nu..sub.C.dbd.O 1720, 1640 ##STR143## Oily material .nu..sub.C.dbd.O 1730, 1640 ##STR144## Oily material .nu..sub.C.dbd.O 1730, 1640 ##STR145## Oily material .nu..sub.C.dbd.O 1720, 1640 ##STR146## Oily material .nu..sub.C.dbd.O 1720, 1640 ##STR147## Oily material .nu..sub.C.dbd.O 1730, 1640 ##STR148## Oily material .nu..sub.C.dbd.O 1730, 1640 ##STR149## Oily material .nu..sub.C.dbd.O 1720, 1640 ##STR150## Oily material .nu..sub.C.dbd.O 1730, 1650 ##STR151## m.p. 105-107.degree. C. .nu..sub.C.dbd.O 1730, 1650 ##STR152## Oily material .nu..sub.C.dbd.O 1720, 1645 ##STR153## Oily material .nu..sub.C.dbd.O 1700-1740 ##STR154## Oily material .nu..sub.C.dbd.O 1730, 1660-1630 ##STR155## Oily material .nu..sub.C.dbd.O 1720, 1640 ##STR156## Oily material .nu..sub.C.dbd.O 1730, 1650 ##STR157## Oily material .nu..sub.C.dbd.O 1730, 1650 ##STR158## Oily material .nu..sub.C.dbd.O 1730, 1650 ##STR159## m.p. 150.degree. C. (decomp.) .nu..sub.C.dbd.O 1670, 1720 ##STR160## Oily material .nu..sub.C.dbd.O 1790, 1720-1670 ##STR161## Oily material .nu..sub.C.dbd.O 1790, 1710, 1670 ##STR162## Oily material .nu..sub.C.dbd.O 1790, 1710-1660 ##STR163## Oily material .nu..sub.C.dbd.O 1790, 1710-1660 ##STR164## m.p. 94-95.degree. C. (decomp.) (from CH.sub.2 Cl.sub.2Et.sub.2 O) .nu..sub.C.dbd.O 1790, 1680 ##STR165## Oily material .nu..sub.C.dbd.O 1790, 1720, 1670 ##STR166## m.p. 95-96.degree. C. (decomp.) (from .nu..sub.C.dbd.O 1780, 1660 ##STR167## Oily material .nu..sub.C.dbd.O 1780, 1710-1640 ##STR168## Oily material .nu..sub.C.dbd.O 1780, 1660 ##STR169## m.p. 130-131.degree. C. (decomp.) .nu..sub.C.dbd.O 1780, 1660 ##STR170## Oily material .nu..sub. C.dbd.O 1790, 1720-1665 ##STR171## Oily material .nu..sub.C.dbd.O 1780, 1720-1640 ##STR172## Oily material .nu..sub.C.dbd.O 1780, 1720-1640 ##STR173## Oily material .nu..sub.C.dbd.O 1780, 1720-1640 ##STR174## Crystal .nu..sub.C.dbd.O 1775, 1660-1620 ##STR175## Crystal .nu..sub.C.dbd.O 1785, 1720-1650 ##STR176## Oily material .nu..sub.C.dbd.O 1790, 1720, 1680 ##STR177## m.p. 65-70.degree. C. (decomp.) .nu..sub.C.dbd.O 1785, 1680 ##STR178## m.p. 100-101.degree. C. (decomp.) .nu..sub.C.dbd.O 1725, 1675 ##STR179## m.p. 180-181.degree. C. .nu..sub.C.dbd.O 1740, 1695 ##STR180## m.p. 160-165.degree. C. .nu..sub.C.dbd.O 1740, 1670 ##STR181## Oily material .nu..sub.C.dbd.O 1800, 1750, 1710 ##STR182## m.p. 185-187.degree. C. (decomp.) .nu..sub.C.dbd.O 1730, 1690 ##STR183## Oily material .nu..sub.C.dbd.O 1750, 1710-1685 ##STR184## Oily material .nu..sub.C.dbd.O 1735, 1725, 1710,__________________________________________________________________________ 1675 Note: Et.sub.2 O = CH.sub.3 CH.sub.2 OCH.sub.2 CH.sub.3 AcOBu = CH.sub.3 CCO(CH.sub.2).sub.3 CH.sub.3

The compound represented by the general formula (V) can be easily obtained by reacting, for example, a salt with an alkali metal, an alkaline earth metal or a nitrogen-containing organic base of an amino acid (VII) (any of D-isomer, L-isomer and racemic compound) represented by the general formula (VII)

H.sub.2 N--R--COOH (VII) wherein R is as defined previously, with a reactive derivative in the (thio)carboxyl group of a compound represented by the general formula (III) in a solvent inert to the reaction in the presence of an acid-binding agent. Preferable examples of the compound of formula (V) are D-isomers, L-isomers and racemic compounds of the following compounds, though it is needless to say that the examples are not limitative; .alpha.-(4-Acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Chloroacetyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Dichloroacetyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Palmitoyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Caproyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Capryloyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Enanthoyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Cyclohexanecarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Benzoyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-p-Chlorobenzoyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-p-Methoxybenzoyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-[4-(3,4,5-Trimethoxybenzoyl)-2-oxo-1-piperazinocarbonylamino]phenylacetic acid .alpha.-[4-(2,4-Dichlorobenzoyl)-2-oxo-1-piperazinocarbonylamino]phenylacetic acid .alpha.-(4-Acetyl-3-methyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Methanesulfonyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Acetylaminocarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Phenylaminocarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Ethoxycarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Pivaloyloxymethyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Hexyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Butyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Butyl-6-methyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Octyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(3-Oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(2,5-Dimethyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(5-Methyl-3-oxo-1-piperazinocarbonylaminophenylacetic acid .alpha.-(2-Ethoxycarbonylmethyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(2-Methyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Acetyl-2-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Phenylaminocarbonyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Methyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Butyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Ethyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Isopropyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Pentyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-iso-Pentyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Hexyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4n-Heptyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Octyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Dodecyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Cyclopentyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(2-Methyl-4-n-butyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Butyl-5-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Butyl-6-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(2-Phenyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Benzyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Carbamoyl-2-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.(4-.beta.-Hydroxyethyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Allyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-.alpha.-Methylallyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4.beta.-Methylally-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-[4-(Trans-2-butenyl)-3-oxo-1piperazinocarbonylamino]phenylacetic acid .alpha.-(4-Morpholinomethyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Ethyl-3-oxo-1-piperazinocarbonylamino)propionic acid .alpha.-(4-Acetyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Benzoyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Methyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Benzyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Acetoxyethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Butyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Isopropyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Pentyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Hexyl-2,3dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Heptyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-n-Octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Allyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Phenyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-.beta.-Chloroethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Pyrrolidinoethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Methyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetic acid .alpha.-(4-Ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetic acid .alpha.-(6-Methyl-4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4,6-Dimethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Ethyl-2,3-dioxo-1-piperazinothiocarbonylamino)phenylacetic acid .alpha.-(4-Methyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetic acid .alpha.-(4-Ethyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetic acid .alpha.-(4-n-Propyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetic acid .alpha.-(4-n-Butyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetic acid .alpha.-(4-Methyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid .alpha.-(4-Ethyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid .alpha.-(4-n-Propyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid .alpha.-(4-n-Butyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid .alpha.-(2,2-Pentamethylene-3,5-dioxo-1-piperzinocarbonylamino)phenylacetic acid .alpha.-(4-Benzyl-2,2-pentamethylene-3,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-.beta.,.beta.,.beta.-Trichloroethoxycarbonyl-2,2-pentamethylene-3,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(3,5-Dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(2-Methyl-2-phenyl-3,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Benzyl-2-methyl-3,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid .alpha.-(4-Methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid

As the rective derivative in the carboxyl group of the compound represented by the general formula (V), there is used a reactive derivative of a carboxylic acid which is ordinarily used in the synthesis of acid amides. Such reactive derivative includes, for example, acid halides, acid anhydrides, mixed acid anhydrides with organic or inorganic acids, active acid amides, acid cyanides, active esters, etc. Particularly, acid chlorides, mixed acid anhydrides and active acid amides are preferable. Examples of the mixed acid anhydrides are mixed acid anhydrides with substituted acetic acids, alkyl carbonic acids, aryl carbonic acids and aralkyl carbonic acids; examples of the active esters are cyanomethyl esters, substituted phenyl esters, substituted benzyl esters, substituted thienyl esters, etc.; and examples of the active acid amides are N-acyl saccharins, N-acyl imidazoles, N-acyl benzoylamides, N,N-dicyclohexyl-N-acylureas, N-acyl sulfonamides, etc.

Compounds of formula (VI) can be obtained by, for example, process (1) or (2). Some of the compounds obtained by process (3) can further be used as the starting compounds in process (3). Any of D-, L- and racemic compounds of formula (VI) may be used.

The modes of practice of the processes (1), (2) and (3) are explained below.

The processes (1) and (2) may be carried out under substantially the same conditions. That is, the compound of formula (II) or (IV) is dissolved or suspended in at least one inert solvent selected from for example, water, acetone, tetrahydrofuran, dioxane, acetonitrile, dimethylformamide, methanol, ethanol, methoxyethanol, diethyl ether, isopropyl ether, benzene, toluene, methylene chloride, chloroform, ethyl acetate, methyl isobutyl ketone and the like. The resulting solution or suspension is reacted with a reactive derivative of the compound of formula (III), or with the compound of formula (V) or a reactive derivative in the carbonyl group of the compound of formula (V) in the presence or absence of a base at a temperature in the range from -60.degree. to 80.degree. C., preferably from -40.degree. to 30.degree. C. The reaction time is ordinarily 5 minutes to 5 hours. Examples of the base used in the above reaction are inorganic bases such as alkali hydroxides, alkali hydrogencarbonates, alkali carbonates, alkali acetates, etc.; tertiary amines such as trimethylamine, triethylamine, tributylamine, pyridine, N-methylpiperidine, N-methylmorpholine, lutidine, collidine, etc.; and secondary amines such as dicyclohexylamine, diethylamine, etc. When the compound of formula (V) is used in the form of a free acid or salt in the process (2), the reaction of the process (2) may be effected in the presence of a dehydrating condensing agent such as N,N-dicyclohexyl carbodiimide, N-cyclohexyl-N'-morpholinoethyl carbodiimide, N,N'-diethyl carbodiimide, N,N'-carbonyl di (2-methylimidazole), a trialkyl ester of phosphorous acid, ethyl ester of polyphosporic acid, phosphorus oxychloride, phosphorus trichloride, 2-chloro-1,3,2-dioxaphospholane or oxazolyl chloride. The salt of the compound of formula (V) includes alkali metal salts, alkaline earth metal salts, ammonium salts, and salts with organic bases such as trimethylamine, dicyclohexylamine and the like.

The process (3) is carried out in the manner described below.

When B in the formula (VI) is a group other than a hetero aromatic N-oxide thio group having a thio groups on the carbon atom adjacent to the N-oxide group in the molecule, the compound of formula (VI) is reacted with the compound of formula (VII) or a tertiary amine in at least one solvent selected from, for example, water, methanol, ethanol, propanol, isopropanol, butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, tetrahydrofuran, dioxane, acetonitrile, ethyl acetate, methoxyethanol, dimethoxyethane, dimethylformamide, dimethyl sulfoxide, dichloromethane, chloroform, a dichloroethane, and the like. The above-mentioned reaction is preferably effected in a strongly polar solvent such as water or the like. In this case, the pH of the reaction solution is advantageously maintained at 2 to 10, preferably 4 to 8. The desired pH may be attained by addition of a buffer solution such as sodium phosphate. The reaction conditions are not particularly limited, though the reaction is ordinarily conducted of 0.degree. to 100.degree. C. over a period of several hours to tens hours. When B in the formula (VI) is a hetero aromatic N-oxide thio group having a thio group on the carbon atom adjacent to the N-oxide group in the molecule, the compound of formula (VI) is reacted with the compound of formula (VII) in the above-mentioned solvent in the presence of a cupric compound. This reaction is particularly useful where an alcohol is used such as methyl alcohol, ethyl alcohol, propyl alcohol, isopropyl alcohol, n-butyl alcohol, benzyl alcohol, ethylene glycol or the like as the compound of formula (VII). In this case, the reaction proceeds smoothly by using an excess of the alcohol per se to allow it to act as the reaction medium, too. The cupric compound used in this process includes organic and inorganic ones, such as cupric chloride, bromide, fluoride, nitrate, sulfate, borate, phosphate, cyanide, formate, acetate, propionate, citrate, tartarate, benzoate, salicylate and the like. The amount of the cupric compound used is preferably 1/2 mole per mole of the compound of formula (VI). The reaction temperature and the reaction time may be varied depending upon the kinds of compound of formula (VI), cupric compound and compound of formula (VII), though they are usually selected from the range of 0.degree. to 100.degree. C. and the range of several minutes to several days, respectively.

The reaction conditions to be adopted in the processes (1), (2) and (3) are not limited to those mentioned above, and can be properly varied depending upon the kinds of reaction reagents.

Further, the non-toxic salts of the general formula (I), in which R.sup.1 is a salt-forming cation, can be easily obtained according to an ordinary procedure from compounds of the general formula (I), in which R.sup.1 is a hydrogen atom or a blocking group.

Thus, among the compounds of formula (I) of the present invention, the penicillins can be easily obtained according to any of the aforesaid processes (1) and (2), while the cephalosporins can be easily obtained according to either the aforesaid process (1), (2), or (3).

The present penicillins and cephalosporine include concretely the following compounds though are not restricted thereto. The following penicillins can be produced by any of the aforesaid processes (1) and (2), and the following cephalosporins can be produced by any of the aforesaid processes (1), (2) and (3).

Penicillins:

6-[D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-dichloroacetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-enanthoyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6[D(-)-.alpha.-(4-cyclohexanecarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-acetyl-3-methyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-methanesulfonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-hexyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-butyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-butyl-6-methyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-octyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-pivaloyloxymethyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-palmitoyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-capryloyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-caproyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-chloroacetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-benzoyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-p-chlorobenzoyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-p-methoxybenzoyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-{D(-)-.alpha.-[4-(3,4,5-trimethoxybenzoyl)-2-oxo-1-piperazinocarbonylamino]phenylacetamido}penicillanic acid, 6-{D(-)-.alpha.-[4-(2,4-dichlorobenzoyl)-2-oxo-1-piperazinocarbonylamino]phenylacetamido}penicillanic acid, 6-[D(-)-.alpha.-(4-acetylaminocarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-phenylaminocarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-ethoxycarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-butyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-ethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-isopropyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-pentyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-iso-pentyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(2-methyl-4-n-butyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-butyl-5-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-butyl-6-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-benzyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-.beta.-hydroxyethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-acetyl-2-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-carbamoyl-2-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(2,5-dimethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(5-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(2-ethoxycarbonylmethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(2-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-ethyl-3-oxo-1-piperazinocarbonylamino)propionamido]penicillanic acid, 6-[D(-)-.alpha.-(4-allyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-methylallyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-.beta.-methylallyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-{D(-)-.alpha.-[4-(trans-2-butenyl)-3-oxo-1-piperazinocarbonylamino]phenylacetamido}penicillanic acid, 6-[D(-)-.alpha.-(4-n-hexyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-heptyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-octyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-dodecyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-cyclopentyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-phenylaminocarbonyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(2-phenyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-morpholinomethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-acetyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-benzoyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-methyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-benzyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-iso-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-acetoxethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-allyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-phenyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-.beta.-chloroethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(6-methyl-4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4,6-dimethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-pentyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-hexyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-heptyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinothiocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetamido]penicillanic acid, 6-[DL-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetamido]penicillanic acid, 6-[DL-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetamido]penicillanic acid, 6-[DL-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetamido]penicillanic acid, 6-[DL-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(2,2-pentamethylene-3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(2-methyl-2-phenyl-3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-benzyl-2,2-pentamethylene-3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-.beta.,.beta.,.beta.-trichloroethoxycarbonyl-2,2-pentamethylene-3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, 6-[D(-)-.alpha.-(4-benzyl-2-methyl-2-phenyl-3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, pivaloyloxymethyl 6-[D(-)-.alpha.-(2-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]pencillanate, phthalidyl 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, phthalidyl 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, phthalidyl 6-[D(-)-.alpha.-(4-iso-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, phthalidyl 6-[D(-)-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, methoxymethyl 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, methoxymethyl 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, methoxymethyl 6-[D(-)-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, methoxymethyl 6-[D(-)-.alpha.-(4-iso-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, methoxymethyl 6-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, pivaloyloxymethyl 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, pivaloyloxymethyl 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, pivaloyloxymethyl 6-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, .beta.-piperidinoethyl 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, .beta.-piperidinoethyl 6-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, .beta.-morpholinoethyl 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, .beta.-morpholinoethyl 6-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanate, etc.

Cephalosporins:

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-pentyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-hexyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-heptyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-iso-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinothiocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinothiocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-phenyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-6-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4,6-dimethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-phenyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-phenyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)propionamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-azidomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1,3,4-triazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-oxadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[3-(2,6-dimethyl-5-oxo-2,5-dihydro-1,2,4-triazinyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(4-methyloxazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(4-methylthiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(pyridyl-1-oxide)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-(2-thiazolinylthiomethyl)-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methylimidazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-(2-pyrimidinylthiomethyl)-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[3-(6-methylpyridazinyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[1-(4-methylpiperazino)thiocarbonylthiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(3-methylisoxazolyl)carbonylthiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-ethoxythiocarbonylthiomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethoxycarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-n-hexyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methanesulfonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-acetylaminocarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-acetyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methanesulfonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-acetylaminocarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-ethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-acetyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, methoxymethyl 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylate, etc.

The susceptible test of typical compounds among the compounds of the present invention are shown below.

(1) The minimum inhibitory concentrations (MIC) of the compounds against different standard strains are shown in Tables 3 and 4.

The minimum inhibitory concentration (MIC) was determined by the plate method disclosed in "Chemotherapy" (Japan), Vol. 16, (1968), pages 98-99. The culture medium used was a Heart infusion agar (pH 7.4). The number of the cells per plate used in the inoculum was 10.sup.4 (10.sup.6 cells/ml).

TABLE 3 Pseudomonas Proteus Compound Staphylococcus Escherichia aeruginosa Klebsiella vulgaris No. Compound aureus 209p coli NIHJ I.F.O. pneumoniae 3027 (Control) ##STR185## <1.57 <1.57 >200 50 >200 ##STR186## <1.57 <1.57 50 >200 <1.57 ##STR187## 3.13 1.57 50 >200 0.79 1 ##STR188## <1.57 <1.57 25 12.5 3.13 2 ##STR189## <1.57 <1.57 50 12.5 6.25 3 ##STR190## <1.57 <1.57 100 3.13 3.13 4 ##STR191## <1.57 <1.57 25 12.5 3.13 5 ##STR192## <1.57 <1.57 25 12.5 <1.57 6 ##STR193## 3.13 3.13 50 6.25 6.25 7 ##STR194## <1.57 <1.57 200 12.5 6.25 8 ##STR195## <1.57 <1.57 100 6.25 3.13 9 ##STR196## <1.57 3.13 100 3.13 3.13 10 ##STR197## <1.57 <1.57 100 6.25 3.13 11 ##STR198## <1.57 <1.57 50 50 6.25 12 ##STR199## <1.57 3.13 50 6.25 12.5 13 ##STR200## <1.57 <1.57 25 <1.57 <1.57 14 ##STR201## <1.57 <1.57 25 3.13 <1.57 15 ##STR202## <1.57 3.13 50 6.25 6.25 16 ##STR203## <1.57 <1.57 12.5 1.57 <1.57 17 ##STR204## <1.57 <1.57 12.5 50 6.25 18 ##STR205## <1.57 <1.57 12.5 25 3.13 19 ##STR206## <1.57 <1.57 12.5 50 3.13 20 ##STR207## 3.13 <1.57 12.5 25 3.13 21 ##STR208## <0.79 1.57 25 25 3.13 22 ##STR209## <1.57 <1.57 50 12.5 6.25 23 ##STR210## <1.57 <1.57 25 6.25 3.13 24 ##STR211## 3.13 <1.57 50 50 25 25 ##STR212## <1.57 <1.57 25 50 3.13 26 ##STR213## <1.57 <1.57 25 25 12.5 27 ##STR214## <1.57 <1.57 25 25 3.13 28 ##STR215## <1.57 <1.57 25 25 3.13 (trans-) 29 ##STR216## 3.13 <1.57 12.5 3.13 3.13 30 ##STR217## <1.57 <1.57 25 6.25 3.13 31 ##STR218## <1.57 <1.57 12.5 6.25 <1.57 32 ##STR219## <1.57 <1.57 12.5 12.5 6.25 33 ##STR220## <1.57 <1.57 50 6.25 3.13 34 ##STR221## 1.57 3.13 100 50 50 35 ##STR222## 1.57 6.25 100 25 25 36 ##STR223## <1.57 <1.57 6.25 <1.57 <1.57 37 ##STR224## <1.57 <1.57 6.25 6.25 <1.57 38 ##STR225## 0.4 <0.1 6.25 3.13 0.4 39 ##STR226## 0.4 <0.1 6.25 1.57 0.4 40 ##STR227## 0.4 <0.1 6.25 3.13 0.4 41 ##STR228## <1.57 <1.57 25 6.25 <1.57 42 ##STR229## 1.57 <1.57 12.5 6.25 <1.57 43 ##STR230## <1.57 <1.57 6.25 1.57 <1.57 44 ##STR231## < 1.57 <1.57 6.25 <1.57 <1.57 45 ##STR232## 0.79 <0.1 12.5 0.79 0.4 46 ##STR233## 0.2 <0.1 6.25 0.4 0.4 47 ##STR234## <1.57 <1.57 6.25 <1.57 <1.57 48 ##STR235## <0.4 <0.4 6.25 25 <0.4 49 ##STR236## <0.4 0.79 12.5 12.5 1.57 50 ##STR237## <0.79 <0.79 6.25 6.25 <0.79 51 ##STR238## <0.4 <0.4 12.5 <0.4 <0.4 52 ##STR239## 0.79 0.79 25 25 1.57 53 ##STR240## 0.79 <0.4 6.25 25 0.79 54 ##STR241## 0.79 0.4 12.5 0.79 0.79 55 ##STR242## 0.79 0.4 6.25 25 0.79 56 ##STR243## <0.4 <0.4 12.5 1.57 0.79 57 ##STR244## <0.79 <0.79 12.5 12.5 3.13 58 ##STR245## <1.57 <1.57 12.5 25 3.13 59 ##STR246## <1.57 <1.57 25 200 3.13 (Note) Sodium Carbenicillin and Sodium Sulbenicillin are regarded as preferable drugs at the level of this technical field, and hence are described for reference. TABLE 4 Com- Staphylo- Pseudomonas Proteus pound coccus Escherichia aeruginosa Klebsiella vulgaris No. Compound aureus 209p coli NIHJ I.F.O. p neumoniae 3027 (Control) ##STR247## (SodiumCephaloglycin) <1.57 <1.57 >200 <1.57 100 ##STR248## (SodiumCephalothin) <1.57 <1.57 >200 <1.57 100 ##STR249## (SodiumCephazolin) <1.57 <1.57 >200 1.57 200 ##STR250## (Cephaloridine) <1.57 >3.13 200 3.13 200 60 ##STR251## 0.79 <0.1 25 3.13 3.13 61 ##STR252## <0.79 <0.79 25 3.13 3.13 62 ##STR253## 0.79 <0.1 50 1.57 3.13 63 ##STR254## <0.79 <0.79 25 <0.79 1.57 64 ##STR255## 0.79 <0.1 25 1.57 3.13 65 ##STR256## 3.13 0.79 25 3.13 3.13 66 ##STR257## <0.79 <0.79 25 0.79 <1.57 67 ##STR258## 6.25 <0.79 100 3.13 12.5 68 ##STR259## 1.57 <0.79 12.5 <0.79 1.57 69 ##STR260## <0.79 <0.79 12.5 <0.4 <0.79 70 ##STR261## <0.79 1.57 100 1.56 <0.79 71 ##STR262## <0.79 <0.79 50 1.56 <0.79 72 ##STR263## <1.57 <0.1 6.25 <0.1 0.4 73 ##STR264## 1.57 <0.1 25 0.2 1.57 74 ##STR265## 1.57 0.2 12.5 0.79 1.57 75 ##STR266## 1.57 <0.4 50 1.57 12.5 76 ##STR267## 1.57 <0.4 50 0.79 3.13 77 ##STR268## 1.57 <0.1 25 0.4 1.57 78 ##STR269## 3.13 <0.1 12.5 0.2 0.79 79 ##STR270## 1.57 <0.1 3.13 0.79 3.13 80 ##STR271## 0.79 <0.1 50 3.13 6.25 81 ##STR272## <1.57 <1.57 12.5 <1.57 <1.57 82 ##STR273## 1.57 <0.1 25 0.79 0.79 83 ##STR274## 1.57 <1.57 25 <1.57 <1.57 84 ##STR275## <1.57 <1.57 25 <1.57 <1.57 (Note) Sodium Cephalothin, Sodium Cephazolin and Cephaloridine are regarded as preferable drugs at the level of this technical field, and hence are set forth for reference. (2) The minimum inhibitory concentrations (MIC) of the compounds against clinical isolates of bacteria are shown in Tables 5 and 6.

MIC was determined in the same manner as in the preceding paragraph (1).

TABLE 5-1______________________________________ Staphlococcus aureus Compound MS 8619 MS 8588 MS 8713 MS 8596______________________________________Con- Sodium <0.4 6.25 3.13 1.56trol Ampicillin Sodium 0.79 6.25 6.25 6.25 Carbenicillin Sodium 3.13 3.13 3.13 3.13 SulbenicillinCompound No. 1 1.57 6.25 3.13 3.13Compound No. 13 0.79 3.13 3.13 3.13Compound No. 14 0.79 3.13 3.13 3.13Compound No. 16 <0.4 3.13 3.13 3.13Compound No. 30 <0.4 1.57 1.57 1.57Compound No. 36 0.79 3.13 6.25 3.13Compound No. 37 0.79 3.13 12.5 3.13Compound No. 38 0.79 3.13 6.25 3.13Compound No. 39 0.79 1.57 3.13 1.57Compound No. 40 0.79 3.13 12.5 3.13Compound No. 45 0.79 3.13 6.25 3.13Compound No. 46 <0.4 1.57 6.25 3.13Compound No. 47 <0.4 3.13 6.25 3.13______________________________________ Staphlococcus aureus Compound MS 8684 F-1 F-2 F-3 F-4 F-5______________________________________Con- Sodium 1.56 12.5 0.79 12.5 50trol Ampicillin Sodium 6.25 6.25 3.13 3.13 12.5 >200 Carbenicillin Sodium 3.13 6.25 3.13 6.25 6.25 >200 SulbenicillinCompound No. 1 3.13 12.5 3.13 6.25 >200Compound No. 13 3.13 12.5 1.57 6.25 200Compound No. 14 3.13 12.5 1.57 6.25 200Compound No. 16 3.13 6.25 0.79 6.25 100Compound No. 30 1.57 3.13 0.79 0.79 3.13 100Compound No. 36 3.13 12.5 3.13 1.57 6.25 >200Compound No. 37 3.13 12.5 1.57 3.13 6.25 >200Compound No. 38 3.13 6.25 1.57 0.79 6.25 >200Compound No. 39 3.13 6.25 1.57 1.57 6.25 >200Compound No. 40 3.13 6.25 3.13 0.79 6.25 >200Compound No. 45 3.13 6.25 1.57 1.57 6.25 >200Compound No. 46 1.57 6.25 1.57 0.79 6.25 >200Compound No. 47 3.13 6.25 1.57 1.57 12.5 >200______________________________________ TABLE 5-2______________________________________ Escherichia coli Compound GN 3481 GN 3435 GN 3452 GN 3465______________________________________Con- Sodium 6.25 3.13 6.25trol Ampicillin Sodium 6.25 6.25 12.5 >200 Carbenicillin Sodium 12.5 6.25 12.5 >200 SulbenicillinCompound No. 1 12.5 6.25 12.5 200Compound No. 13 6.25 3.13 3.13 25Compound No. 14 6.25 6.25 6.25 50Compound No. 16 3.13 1.57 1.57 12.5Compound No. 30 25 12.5 25 50Compound No. 36 3.13 1.57 3.13 100Compound No. 37 6.25 3.13 12.5 200Compound No. 38 3.13 0.79 3.13 50Compound No. 39 1.57 0.79 0.79 25Compound No. 40 1.57 0.79 1.57 50Compound No. 45 1.57 0.79 1.57 25Compound No. 46 3.13 <0.4 0.79 6.25Compound No. 47 1.57 0.79 1.57 6.25______________________________________ Escherichia coli Compound GN 3611 K-1 K-2 K-3 K-4______________________________________Con- Sodium >200 6.25 6.25 >200 12.5trol Ampicillin Sodium >200 6.25 6.25 >200 12.5 Carbenicillin Sodium >200 6.25 12.5 >200 6.25 SulbenicillinCompound No. 1 6.25 25 >200 12.5Compound No. 13 3.13 6.25 100 6.25Compound No. 14 3.13 12.5 200 6.25Compound No. 16 1.57 3.13 50 3.13Compound No. 30 >200 12.5 25 >200 12.5Compound No. 36 >200 3.13 3.13 >200 1.57Compound No. 37 >200 12.5 6.25 >200 3.13Compound No. 38 >200 3.13 3.13 >200 0.79Compound No. 39 >200 1.57 1.57 >200 0.79Compound No. 40 >200 1.57 3.13 >200 0.79Compound No. 45 >200 0.79 1.57 200 0.79Compound No. 46 >200 0.79 0.79 50 <0.4Compound No. 47 >200 1.57 1.57 100 0.79______________________________________ TABLE 5-3__________________________________________________________________________ Pseudomonas aeruginosaCompound GN 1035 GN 376 GN 82 GN 221 GN 1091 GN 1091 GN 2987 GN 163 GN GN__________________________________________________________________________ 383Con- Sodium >200 >200 >200 >200 >200 >200 >200 >200 >200 >200trol Ampicillin Sodium >200 50 100 25 100 200 50 50 50 50 Carbenicillin Sodium 100 50 50 25 50 100 25 50 50 50 SulbenicillinCompound No. 1 100 25 25 25 25 50 25 25 25 50Compound No. 13 50 50 50 50 25 50 12.5 25 50 50Compound No. 14 50 50 25 25 25 50 12.5 25 50 50Compound No. 16 25 25 6.25 25 12.5 12.5 3.13 12.5 12.5 25Compound No. 19 100 12.5 12.5 12.5 12.5 50 12.5 12.5 25 12.5Compound No. 30 50 50 25 50 25 50 12.5 25 50 50Compound No. 36 25 6.25 6.25 3.13 6.25 25 12.5 6.25 12.5 6.25Compound No. 37 50 12.5 6.25 6.25 12.5 25 25 12.5 50 25Compound No. 38 12.5 3.13 3.13 6.25 3.13 12.5 6.25 3.13 6.25 6.25Compound No. 39 12.5 6.25 3.13 3.13 3.13 12.5 3.13 3.13 6.25 6.25Compound No. 40 25 3.13 6.25 6.25 6.25 12.5 3.13 6.25 6.25 6.25Compound No. 45 50 25 12.5 3.13 12.5 25 12.5 12.5 25 25Compound No. 46 50 25 6.25 12.5 12.5 12.5 6.25 6.25 12.5 25Compound No. 47 25 50 12.5 25 12.5 25 12.5 12.5 25 50__________________________________________________________________________ TABLE 5-4__________________________________________________________________________ Pseudomonas aeruginosa Klebsiella pneumoniaeCompound S-1 S-2 S-3 S-4 GN 4117 GN 4081 GN 3850 GN 917__________________________________________________________________________Con- Sodium >200 >200 >200 >200 >200 >200 50 25trol Ampicillin Sodium 200 200 200 200 >200 >200 >200 Carbenicillin Sodium 100 100 100 100 >200 >200 >200 >200 SulbenicillinCompound No. 1 50 100 50 50 200 >200 25 25Compound No. 13 50 50 100 50 25 25 6.25 12.5Compound No. 14 50 50 100 50 50 50 12.5 25Compound No. 16 12.5 25 50 25 25 25 3.13 12.5Compound No. 19 50 50 50 50 >200 >200 100 50Compound No. 30 50 50 100 50 100 100 25 25Compound No. 36 50 12.5 25 50 100 100 12.5 6.25Compound No. 37 200 25 50 100 100 200 25 12.5Compound No. 38 12.5 12.5 12.5 12.5 50 50 6.25 3.13Compound No. 39 12.5 12.5 25 12.5 25 25 3.13 1.57Compound No. 40 12.5 25 25 12.5 50 100 12.5 6.25Compound No. 45 25 25 50 25 25 25 3.13 1.57Compound No. 46 50 50 50 50 12.5 12.5 1.57 0.79Compound No. 47 50 50 50 50 12.5 12.5 3.13 1.57__________________________________________________________________________ TABLE 5-5__________________________________________________________________________ Salmonella Shigella sonnei Shigella flexneri Salmonella typhi typhi-murium__________________________________________________________________________Compound JS 11755 JS 11232 JS 11215 JS 11839 SL 2169 SL 819 SL 2136 SL 858__________________________________________________________________________Con- Sodium 6.25 >200 1.57 0.78 1.56 >200 3.13trol Ampicillin Sodium 12.5 >200 >200 12.5 3.13 6.25 >200 12.5 Carbenicillin Sodium >200 >200 >200 12.5 1.57 6.25 >200 25 SulbenicillinCompound No. 1 12.5 >200 100 3.13 6.25 6.25 >200 12.5Compound No. 13 3.13 12.5 12.5 1.57 3.13 6.25 200 0.79Compound No. 14 6.25 25 25 3.13 3.13 6.25 200 1.57Compound No. 16 1.57 6.25 6.25 0.79 1.57 3.13 100 1.57Compound No. 36 3.13 50 100 3.13 1.57 1.57 >200 6.25Compound No. 37 6.25 100 >200 6.25 3.13 6.25 >200 12.5Compound No. 38 3.13 50 25 1.57 0.79 1.57 200 3.13Compound No. 39 1.57 25 25 0.79 0.79 0.79 100 0.79Compound No. 40 3.13 50 50 3.13 1.57 1.57 >200 6.25Compound No. 45 1.57 25 25 1.57 0.79 1.57 200 0.79Compound No. 46 0.79 12.5 6.25 0.79 0.79 1.57 50 <0.4Compound No. 47 0.79 6.25 6.25 1.57 1.57 3.13 50 <0.4__________________________________________________________________________ TABLE 5-6______________________________________ ProteusCompound mirabilis morganii vulgaris rettgeri______________________________________Con- Sodium <1.57 <1.57 <1.5 200trol Ampicillin Sodium 0.8 0.4 0.8 >200 Carbenicillin Sodium 0.79 <0.4 <0.4 >200 SulbenicillinCompound No. 16 1.56 1.56 0.8 6.25Compound No. 30 3.13 3.13 3.13 12.5Compound No. 36 <0.4 <0.4 <0.4 12.5Compound No. 37 0.79 0.79 <0.4 25Compound No. 38 <0.4 <0.4 <0.4 12.5Compound No. 39 <0.4 <0.4 <0.4 6.25Compound No. 40 <0.4 0.79 <0.4 6.25Compound No. 45 <0.4 0.79 <0.4 6.25Compound No. 46 <0.4 <0.4 <0.4 3.13Compound No. 47 <0.4 <0.4 <0.4 0.79______________________________________ TABLE 6-1__________________________________________________________________________ Staphylococcus aureusCompound MS 8619 MS 8588 MS 8713 MS 8596 MS 8684 F-1 F-2 F-3 F-4 F-5__________________________________________________________________________Con- Sodium 1.56 3.13 3.13 1.56 1.56 3.13 1.56 1.56 3.13 25trol Cephaloglycin Sodium <0.4 <0.4 <0.4 <0.4 <0.4 <0.4 <0.4 <0.4 <0.4 1.56 Cephalothin Sodium <0.4 <0.4 <0.4 <0.4 <0.4 0.78 <0.4 <0.4 <0.4 0.78 Cephazolin Cephalorizine <0.4 <0.4 <0.4 <0.4 <0.4 <0.4 <0.4 <0.4 <0.4 0.78Compound No. 60 0.78 1.56 0.78 0.78 1.56 3.13 0.78 1.56 1.56 50Compound No. 61 1.56 1.56 1.56 1.56 1.56 3.13 1.56 1.56 3.13 50Compound No. 62 0.78 1.56 1.56 0.78 1.56 3.13 0.78 1.56 1.56 12.5Compound No. 63 0.78 1.56 1.56 1.56 1.56 3.13 0.78 1.56 1.56 12.5Compound No. 68 0.78 1.56 3.13 1.56 1.56 1.56 1.56 0.78 6.25 0.78Compound No. 69 0.78 1.56 1.56 1.56 1.56 3.13 1.56 0.78 3.13 0.78Compound No. 72 1.56 1.56 -- 3.13 1.56 1.56 -- -- -- --Compound No. 73 1.56 1.56 -- 3.13 1.56 1.56 -- -- -- --Compound No. 74 0.78 0.78 -- 0.78 0.78 0.78 -- -- -- --Compound No. 77 1.56 1.56 -- 1.56 1.56 1.56 -- -- -- --Compound No. 78 1.56 3.13 -- 3.13 1.56 3.13 -- -- -- --Compound No. 79 1.56 3.13 -- 3.13 1.56 1.56 -- -- -- --Compound No. 82 1.56 3.13 -- 3.13 1.56 3.13 -- -- -- --__________________________________________________________________________ TABLE 6-2__________________________________________________________________________ Escherichia coliCompound GN 3481 GN 3435 GN 3452 GN 3465 GN 3611 K-1 K-2 K-3 K-4__________________________________________________________________________Con- Sodium 3.13 1.56 3.13 12.5 25 1.56 1.56 25 12.5trol Cephaloglycin Sodium 12.5 6.25 12.5 25 50 6.25 6.25 100 25 Cephalothin Sodium 1.56 1.56 1.56 6.25 25 1.56 1.56 >200 3.13 Cephazolin Cephalorizine 3.13 3.13 3.13 50 100 3.13 3.13 200 6.25Compound No. 60 6.25 6.25 12.5 100 >200 6.25 12.5 200 25Compound No. 61 3.13 3.13 6.25 50 200 3.13 6.25 100 6.25Compound No. 62 6.25 6.25 6.25 25 200 6.25 12.5 200 12.5Compound No. 63 3.13 3.13 12.5 25 100 3.13 6.25 50 6.25Compound No. 72 -- 0.1 0.2 1.56 -- -- 0.39 -- 0.39Compound No. 73 -- 0.78 0.78 1.56 -- -- 1.56 -- 0.78Compound No. 74 -- 0.78 1.56 1.56 -- -- 3.13 -- 1.56Compound No. 77 -- 1.56 1.56 6.25 -- -- 3.13 -- 1.56Compound No. 78 -- 0.39 0.39 1.56 -- -- 0.78 -- 0.39Compound No. 79 -- 0.39 0.39 3.13 -- -- 1.56 -- 0.78Compound No. 82 -- 1.56 1.56 6.25 -- -- 6.25 -- 1.56__________________________________________________________________________ TABLE 6-3__________________________________________________________________________ Pseudomonas aeruginosaCompound GN 1035 GN 376 GN 82 GN 221 GN 1091 GN 2565 GN 2987 GN 163 GN 244 GN__________________________________________________________________________ 383Con- Sodium >200 >200 >200 >200 >200 >200 >200 >200 >200 >200trol Cephaloglycin Sodium >200 >200 >200 >200 >200 >200 >200 >200 >200 >200 Cephalothin Sodium >200 >200 >200 >200 >200 >200 >200 >200 >200 >200 Cephazolin Cephalerizine >200 >200 >200 >200 >200 >200 >200 >200 >200 >200Compound No. 60 200 50 50 12.5 50 100 50 50 50 50Compound No. 61 100 12.5 25 6.25 25 50 25 25 25 12.5Compound No. 62 200 100 100 50 100 100 50 50 100 100Compound No. 63 100 50 50 25 50 50 25 25 50 25Compound No. 68 50 12.5 6.25 3.13 12.5 50 12.5 12.5 25 12.5Compound No. 69 50 12.5 12.5 6.25 12.5 50 12.5 12.5 25 25Compound No. 72 25 6.25 6.25 6.25 6.25 25 -- -- 12.5 12.5Compound No. 73 50 12.5 12.5 12.5 12.5 25 -- -- 12.5 12.5Compound No. 74 25 50 25 50 25 50 -- -- 25 50Compound No. 77 50 25 25 12.5 25 50 -- -- 25 25Compound No. 78 25 12.5 12.5 6.25 25 12.5 -- -- 12.5 25Compound No. 79 25 6.25 12.5 6.25 12.5 25 -- -- 12.5 12.5Compound No. 82 50 25 50 25 50 50 -- -- 50 50__________________________________________________________________________ TABLE 6-4__________________________________________________________________________ Pseudomonas aeruginosa Klebsiella pneumoniaeCompound S-1 S-2 S-3 S-4 GN 4117 GN 4081 GN 917__________________________________________________________________________Con- Sodium >200 >200 >200 >200 3.13 3.13 1.56trol Cephaloglycin Sodium >200 >200 >200 >200 6.25 12.5 3.13 Cephalothin Sodium >200 >200 >200 >200 3.13 3.13 1.56 Cephazolin Cephalorizine >200 >200 >200 >200 12.5 12.5 3.13Compound No. 60 200 200 100 100 25 25 6.25Compound No. 61 50 50 50 50 12.5 12.5 6.25Compound No. 62 200 200 200 200 25 12.5 6.25Compound No. 63 100 100 100 100 6.25 6.25 3.13Compound No. 68 25 25 25 25 -- -- 1.56Compound No. 69 25 25 50 50 -- -- 0.78Compound No. 72 12.5 -- -- -- -- 1.56 0.2Compound No. 73 50 -- -- -- -- 1.56 0.78Compound No. 74 50 -- -- -- -- 1.56 1.56Compound No. 77 50 -- -- -- -- 3.13 1.56Compound No. 78 25 -- -- -- -- 3.13 0.39Compound No. 79 25 -- -- -- -- 12.5 0.78Compound No. 82 100 -- -- -- -- 6.25 1.56__________________________________________________________________________ TABLE 6-5__________________________________________________________________________ ProteusCompound mirabilis morganii vulgaris rettgeri__________________________________________________________________________Sodium 3.13 1.56 50 50CephaloglycinCompound No. 60 3.13 3.13 1.56 6.25Compound No. 61 1.56 1.56 0.8 3.13Compound No. 62 6.25 3.13 3.13 6.25Compound No. 63 3.13 3.13 1.56 3.13Compound No. 72 0.78 0.78 0.39 1.56Compound No. 73 0.39 0.78 0.78 3.13Compound No. 74 0.39 0.78 0.78 6.25Compound No. 77 1.56 1.56 0.78 1.56Compound No. 78 1.56 1.56 0.39 1.56Compound No. 79 1.56 1.56 0.39 3.13Compound No. 82 3.13 1.56 0.78 6.25__________________________________________________________________________ (3) Resistant activity against .beta.-lactamase, Pseudomonas aerugionsa GN 238:

The resistant activity of each compound against .beta.-lactamase was measured in the manner described below.

.beta.-Lactamase was prepared from Pseudomonas aeruginosa GN 238. This microorganism was cultured in 100 ml of a medium containing 2 g of yeast extract, 10 g of polypeptone, 2 g of glucose, 7 g of disodium hydrogen phosphate, 2 g of potassium dihydrogen phosphate, 1.2 g of ammonium sulfate and 0.4 g of magnesium sulfate, per liter, in a 500 -ml Erlenmeyer flask for 6 hrs. at 37.degree. C. with shaking. The resulting cells were collected by centrifugation (5,000 r.p.m..times.10 min.), washed three times with 0.1 M phosphate buffer (pH 7.0). Subsequently, the cells were subjected to sonication (20 KH.sub.z, 20 min.) and then centrifuged at 15,000 r.p.m. for 60 min. By using the supernatant of enzyme fluid, the resistance of each compound against .beta.-lactamase was determined by the iodometric assay method. The results obtained were as set forth in Table 7. Each numeral shown in Table 7 is a relative activity value calculated by assuming as 100 the activity of the control Potassium Penicillin G.

TABLE 7-1______________________________________Comparison of resistant activityagainst .beta.-lactamase Relative activityCompound (%)______________________________________Con- Potassium Penicillin G 100trol Sodium Ampicillin 115 Sodium Carbenicillin 116 Sodium Sulbenicillin 50Compound No. 30 3Compound No. 36 14Compound No. 37 15Compound No. 38 15Compound No. 39 15Compound No. 40 15Compound No. 45 16Compound No. 46 12Compound No. 47 1______________________________________ (4) Resistant activity of each cephalosporin compound against .beta.-lactamase, Proteus vulgaris GN 76 and Escherichia freundii GN 346:

The resistant activity of each cephalosporin compound against .beta.-lactamase was measured in the manner described below.

.beta.-lactamase was prepared from Proteus vulgaris GN 76 and Esherichia freundii GN 346. 10 ml of overnight culture in Heart Infusion broth was diluted with 100 ml of a medium containing 2 g of yeast extract, 10 g of polypeptone, 2 g of glucose, 7 g of disodium hydrogen phosphate, 2 g of potassium dihydrogen phosphate, 1.2 g of ammonium sulfate and 0.4 g of magnesium sulfate, per liter, and incubated with shaking at 37.degree. C. After incubation of 2 hrs, Penicillin G (50 .mu.g/ml) was added as the inducer, and further incubated for 2 hrs. The resulting cells were collected by centrifugation (5,000 r.p.m..times.10 min.), washed two times with 0.1 M phosphate buffer (pH 7.0). Subsequently, the cells were subjected to sonication (20 KHz, 5 min.) and then centrifuged at 15,000 r.p.m. for 60 min. By using the supernatant of enzyme fluid, the stability of each compound against .beta.-lactamase was determined by the iodometric assay method. The results obtained were as set forth in Table 7-2. Each numeral shown in Table 7-2 is a relative activity value calculated by assuming as 100 the activity of the control Cephalorizine.

TABLE 7-2______________________________________Comparison of relative activity against .beta.-lactamase Relative activity (%) Pr. vulgaris E. freundiiCompound GN 6 GN 346______________________________________Con- Cephalorigine 100 100trol Sodium Cephalexin 150 29 Sodium Cephaloglycin 1300 0.3 Sodium Cephalothin 105 17 Sodium Cephazoline 440 150Compound No. 63 6 0.2Compound No. 67 5 0.4Compound No. 69 4 0.1Compound No. 72 7 0.2Compound No. 73 4 0.1Compound No. 74 5 0.03Compound No. 76 11 1Compound No. 81 6 0.4Compound No. 84 10 1______________________________________

From Tables 3 to 6, it is understood that the compounds of the present invention have a broader antibacterial spectrum and more excellent antibacterial activity against not only Pseudomonas aeruginosa, Klebsiella pneumoniae, and Proteus species but also many drug-resistant bacteria than the control ampicillin and cephaloglycin, i.e. compounds having an amino group at the .alpha.-position of the acyl group. It is also understood from Table 7-1 and Table 7-2 that the compounds of the present invention are far higher in resistance to .beta.-lactamase than the control drugs.

As is clear from the above results, the compounds represented by the formula (Ie), among the compounds of the present invention, show prominent effects, and particularly preferable compounds are those of the formula (Ie), in which A represents a hydrogen atom, or an unsubstituted or substituted alkyl, alkenyl, aryl or aralkyl group; and R.sup.2 and R.sup.3 represent individually a hydrogen atom or an alkyl group.

The present penicillins and cephalosporins have generally low toxicity. For example, 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid and 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid have LD.sub.50 (i.v. in mouse having a weight of 19.+-.1 g) greater than 5 g/kg.

The compounds of formula (I) of the present invention may be administered not only in the form of free acids but also in the form of non-toxic salts or physiologically acceptable esters. Further, the compounds, which are in the form of physiologically unacceptable esters, are ordinarily put into uses after bringing them to the form of free acids or non-toxic salts by removing the ester-forming group according to a conventional procedure known in this technical field.

The compounds of the present invention can be administered to humans and animals after formulating them into a physiological form such as tablet, capsule, syrup, injection or the like which is usually adopted in the case of penicillin and cephalosporin type drugs.

Procedures for producing the compounds of the present invention are shown below with reference to examples.

EXAMPLE 1

(1) To a mixture comprising 2.5 g of 1-acetyl-3-oxo-piperazine, 3.45 g of triethylamine and 20 ml of anhydrous dioxane was added a solution of 3.71 g of trimethylchlorosilane in 10 ml of anhydrous dioxane. The resulting mixture was refluxed for 17 hours and cooled to deposit triethylamine hydrochloride, which was then removed by filtration. The filtrate was dropped at -40.degree. to -30.degree. C. into a solution of 1.8 g of phosgene in 30 ml of anhydrous methylene chloride. After the dropping, the resulting mixture was elevated in temperature, and reacted at room temperature for 30 minutes. Subsequently, the excess phosgene and the solvent were removed by distillation under reduced pressure to obtain 3.5 g of pale brown, oily 4-acetyl-2-oxo-1-piperazinocarbonyl chloride.

IR (film) cm.sup.-1 : .nu..sub.C=0 1790, 1710, 1640.

(2) A suspension of 1.0 g of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 20 ml of tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by gradual addition of triethylamine with stirring, and then cooled to 0.degree. C. Into the thus treated suspension was dropped a solution of 900 mg of the aforesaid 4-acetyl-2-oxo-piperazinocarbonyl chloride in 5 ml of tetrahydrofuran at said temperature over a period of 30 minutes. During this period, the pH of the suspension was maintained at 7.5 to 8.0 by gradual addition of triethylamine. Subsequently, the temperature of resulting mixture was elevated to 5.degree. to 10.degree. C., and the mixture was reacted at said temperature for 1 hour while maintaining the pH thereof at 7.5 to 8.0 addition of triethylamine. After the reaction, the tetrahydrofuran was removed by reduced pressure distillation, and the residue was dissolved in a mixed solvent comprising 30 ml of ethyl acetate and 10 ml of water. The resulting solution was adjusted to a pH of 1.5 to 2 by addition of dilute hydrochloric acid with ice-cooling, and then the organic layer was separated off. The aqueous layer was re-extracted with 20 ml of ethyl acetate, and the resulting organic layer was combined with the aforesaid organic layer. The combined organic layer was washed with water, dried over anhydrous magnesium sulfate, and then ice-cooled. Into this organic layer was dropped a solution of 470 mg of a sodium salt of 2-ethylhexonic acid in 20 ml of ethyl acetate to deposit white crystals. The deposited crystals were collected by filtration, washed with ethyl acetate and then dried to obtain 1.4 g of a sodium salt of 6-[D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 205.degree. C. (decomp.), yield 94%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1760 (lactam), 1600-1700 (--COO.sup..crclbar., --CON<).

NMR: [(CD.sub.3).sub.2 SO+D.sub.2 O].tau. values: 2.73 (5H), 4.35 (1H), 4.75 (2H), 5.75 (1H), 5.84 (2H), 6.42 (4H), 8.03 (3H), 8.52 (3H), 8.64 (3H).

The above-mentioned operation was repeated, except that the 4-acetyl-2-oxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 8, to obtain the respective objective compounds as shown in Table 8. The structure of each objective compound was confirmed by IR and NMR.

TABLE 8__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ ##STR276## ##STR277## ##STR278## ##STR279## ##STR280## ##STR281## ##STR282## ##STR283## ##STR284## ##STR285## ##STR286## ##STR287## ##STR288## ##STR289## ##STR290## ##STR291## ##STR292## ##STR293## ##STR294## ##STR295##__________________________________________________________________________

EXAMPLE 2

(1) Into a solution of 1.74 g of a sodium salt of D(-)-.alpha.-aminophenylacetic acid in 30 ml of tetrahydrofuran containing 20% by volume of water which had been cooled to 0.degree. C., a solution of 2.5 g of 4-acetyl-2-oxo-1-piperazinocarbonyl chloride in 5 ml of tetrahydrofuran was dropped at said temperature over a period of 30 minutes. During this period, the pH of the reaction solution was maintained at 11.0 to12.0 by gradual addition of a 10% aqueous sodium hydroxide solution. Subsequently, the temperature of the resulting mixed solution was elevated to 5.degree. to 10.degree. C., and the solution was reacted at room temperature for 2 hours while maintaining the pH thereof at 10.0 to 11.0 by addition of a 10% aqueous sodium hydroxide solution. After the reaction, the tetrahydrofuran was removed by reduced pressure distillation. The residue was dissolved in a mixed solvent comprising 20 ml of water and 50 ml of ethyl acetate, and the resulting solution was adjusted to a pH of 1.0 to 1.5 by addition of dilute hydrochloric acid with ice-cooling. Subsequently, the organic layer was separated off, washed with water and then dried over anhydrous magnesium sulfate. To this organic layer, a solution of 1.66 g of a sodium salt of 2-ethylhexonic acid in 20 ml of ethyl acetate was added to deposit white crystals. The deposited crystals were collected by filtration, sufficiently washed with ethyl acetate and then dried to obtain 1.89 g of a sodium salt of D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid, m.p. 115.degree. C. (decomp.), yield 52%.

IR (KBr) cm.sup.-1 : .nu..sub.C=0 1690, 1650-1600.

(2) To a suspension in 15 ml of anhydrous acetone of 833 mg of the above-mentioned sodium salt of D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetic acid was added 10 mg of N-methylmorpholine. The resulting mixture was cooled to -20.degree. to -15.degree. C. and a solution of 286 mg of ethyl chlorocarbonate in 5 ml of anhydrous acetone was dropped into said mixture over a period of 5 minutes. Subsequently, the mixture was stirred at said temperature for 60 minutes. Into the thus treated mixture, a solution of 646 mg of a triethylamine salt of 6-aminopenicillanic acid in 30 ml of anhydrous methylene chloride was dropped at -40.degree. to -30.degree. C. over a period of 10 minutes. Thereafter, the mixture was reacted with stirring at -30.degree. to -20.degree. C. for 60 minutes, at -20.degree. to 10.degree. C. for 30 minutes, and at -10.degree. to 0.degree. C. for 30 minutes. After the reaction, the organic solvent was removed by reduced pressure distillation. The residue was dissolved in a mixed solvent comprising 50 ml of ethyl acetate and 20 ml of water, and the resulting solution was adjusted to a pH of 1.5 to 2.0 by addition of dilute hydrochloric acid with ice-cooling. Subsequently, the organic layer was separated off, sufficiently washed with water and then dried over anhydrous magnesium sulfate, and the ethyl acetate was removed by reduced pressure distillation. The residue was dissolved in 50 ml of acetone, and the resulting solution was mixed with a solution of 340 mg of a sodium salt of 2-ethylhexonic acid in 20 ml of acetone with ice-cooling to deposit white crystals. The deposited crystals were collected by filtration, sufficiently washed with acetone and then dried to obtain 1.16 g of a sodium salt of 6-[D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 205.degree. C. (decomp.), yield 94%.

EXAMPLE 3

(1) To a mixture comprising 1.0 g of 1 -palmitoyl-3-oxo-piperazine, 0.6 g of triethylamine and 20 ml of anhydrous dioxane was added a solution of 0.65 g of trimethylchlorosilane in 10 ml of anhydrous dioxane. The resulting mixture was refluxed for 16 hours and cooled to deposit triethylamine hydrochloride, which was then removed by filtration. The filtrate was dropped at -40.degree. to -30.degree. C. into a solution of 0.6 g of phosgene in 30 ml of anhydrous methylene chloride. After the dropping, the temperature of the resulting mixture was elevated and the mixture was reacted at room temperature for 30 minutes. Subsequently, the excess phosgene and the solvent were removed by reduced pressure distillation to obtain 1.1 g of pale yellow, oily 4-palmitoyl-2-oxo-1-piperazinocarbonyl chloride.

IR (film) cm.sup.-1 : .nu..sub.C=0 1740, 1660, 1640.

(2) A suspension of 1.0 g of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 20 ml of tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by gradual addition of triethylamine with stirring, and then cooled to 0.degree. C. Into the thus treated suspension, a solution of 1.27 g of the aforesaid palmitoyl-2-oxo-1-piperazinocarbonyl chloride in 5 ml of tetrahydrofuran was dropped at said temperature over a period of 30 minutes. During this period, the pH of the suspension was maintained at 7.5 to 8.0 by gradual addition of treithylamine. Subsequently, the temperature of the resulting mixture was elevated to 5.degree. to 10.degree. C., and the mixture was reacted at said temperature for 1 hour while maintaining the pH thereof at 7.5 to 8.0 by addition of triethylamine. After the reaction, the tetrahydrofuran was removed by reduced pressure distillation, and the residue was dissolved in a mixed solvent comprising 30 ml of ethyl acetate and 10 ml of water. The resulting solution was adjusted to a pH of 1.0 to 2.0 by addition of dilute hydrocloric acid with ice-cooling, and then the organic layer was separated off. The aqueous layer was re-extracted with 20 ml of ethyl acetate, and the resulting organic layer was combined with the aforesaid organic layer. The combined organic layer was washed with water, and dried over anhydrous magnesium sulfate. This organic layer was concentrated under reduced pressure to remove the solvent, and the concentrate was charged into 10 ml of diisopropyl ether to deposit crystals. Thereafter, the crystals were collected by filtration to obtain 1.65 g of white crystals of 6-[D(-)-.alpha.-(4-palmitoyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 121.degree.-123.degree. C. (decomp.), yield 80%.

IR (KBr) cm.sup.-1 : .nu..sub.C=0 1770 (lactam), 1730 (--COOH), 1660-1630 (--CON<).

The above-mentioned operation was repeated, except that the 4-palmitoyl-2-oxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 9, to obtain respective objective compounds as shown in Table 9. The structure of each objective compound was confirmed by IR and NMR.

TABLE 9__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ ##STR296## ##STR297## ##STR298## ##STR299## ##STR300## ##STR301## ##STR302## ##STR303## ##STR304## ##STR305## ##STR306## ##STR307## ##STR308## ##STR309## ##STR310## ##STR311## ##STR312## ##STR313## ##STR314## ##STR315## ##STR316## ##STR317##__________________________________________________________________________

EXAMPLE 4

(1) To a solution of 6.4 g of 1-formyl-3-oxo-piperazine in 10 ml of anhydrous dimethylformamide was added 2.7 g of a sodium hydride (purity 53%) with ice-cooling, and the resulting mixture was reacted at room temperature for 1 hour. Subsequently, the mixture was incorporated with 7.1 g of methyl iodide and reacted for 10 hours. After the reaction, the dimethylformamide was removed by reduced pressure distillation to obtain 1-formyl-4-methyl-3-oxo-piperazine. This piperazine was dissolved in 70 ml of a 50% aqueous actone solution containing 2.2 g of sodium hydroxide, and the resulting solution was reacted at room temperature for 3 hours. Thereafter, the solvent was removed by distillation under reduced pressure, and the residue was charged into acetone to deposit insolubles. The insolubles were separated by filtration, and the acetone was removed from the filtrate the distillation under reduced pressure. Subsequently, the residue was subjected to reduced pressure distillation to obtain 5.2 g of 1-methyl-2-oxo-piperazine, b.p. 104.degree. C./4 mmHg, yield 91%.

(2) Into a solution of 1.9 g of phosgene in 20 ml of anhydrous dioxane was dropped at 10.degree. C. 20 ml of an anhydrous dioxane solution containing 2.0 g of 1-methyl-2-oxo-piperazine and 1.95 g of triethylamine, upon which reaction took place to deposit white crystals of triathylamine hydrochloride. The deposited crystals were removed by filtration, and the filtrate was concentrated to dryness to obtain 3.0 g of pale yellow, oily 4-methyl-3-oxo-1-piperazinocarbonyl chloride.

IR (film) cm.sup.-1 : .nu..sub.C=0 1710, 1630.

(3) A suspension of 4.0 g of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in40 ml of tetrahydrofurna containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by gradual addition of triethylamine with stirring, and then cooled to 0.degree. C. Into the thus treated suspension, 10 ml of a tetrahydrofuran solution containing 2.2 g of the aforesaid 4-methyl-3-oxo-1-piperazinocarbonyl chloride was dropped. During this period, the pH of the suspension was maintained at 7.5 to 8.5 by gradual addition of triethylamine. Subsequently, the resulting mixture was reacted at said temperature for 30 minutes, and the temperature thereof was elevated at 10.degree. to 15.degree. C., after which the mixture was further reacted at said temperature for 90 minutes while maintaining the pH thereof at 7.5 to 8.0 by addition of triethylamine. After the reaction, the tetrahydrofuran was removed by distillation under reduced pressure, and the residue was dissolved in 30 ml of water. The resulting solution was washed with ethyl acetate, and then the aqueous layer was separated off. This aqueous layer was ice-cooled and then adjusted to a pH of 1.5 by addition of dilute hydrochloric acid to deposit white crystals. The deposited crystals were collected by filtration, washed several times with a small amount of water, dried, and then dissolved in 100 ml of acetone. To the resulting solution was added 1.9 g of a sodium salt of 2-ethylhexonic acid with ice-cooling to deposit white crystals, which were then collected by filtration to obtain 5.4 g of a sodium salt of 6-[D(-)-.alpha.-(4-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 195.degree. C. (decomp.), yield 92%.

IR (KBr) cm.sup.-1 :.nu..sub.C=0 1760 (lactam), 1600-1660 (--CON<,--COO.crclbar.).

NMR [(CD.sub.3).sub.2 SO+D.sub.2 O] .tau. values: 2.62 (5H), 4.48 (1H), 4.56 (2H), 5.97 (3H), 6.63-6.39 (4H), 7.13 (3H), 8.46 (3H), 8.55 (3H).

The above-mentioned operation was repeated, except that the 4-methyl-3-oxo-1-piperazinocarbonyl chlordie was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 10, to obtain respective objective compounds as shown in Table 10. The structure of each objective compound was confirmed by IR and NMR.

TABLE 10__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ ##STR318## ##STR319## ##STR320## ##STR321## ##STR322## ##STR323## ##STR324## ##STR325## ##STR326## ##STR327## ##STR328## ##STR329## ##STR330## ##STR331## ##STR332## ##STR333## ##STR334## ##STR335## ##STR336## ##STR337## ##STR338## ##STR339## ##STR340## ##STR341## ##STR342## ##STR343## ##STR344## ##STR345## ##STR346## ##STR347## ##STR348## ##STR349## ##STR350## ##STR351##__________________________________________________________________________

EXAMPLE 5

(1) A solution of 1.0 g of a sodium salt of D(-)-.alpha.-aminophenyl acetic acid in 20 ml of tetrahydrofuran containing 20% by volume of water was cooled to 0.degree. to 5.degree. C. To this solution was added 1.2 g of 2-methyl-3-oxo-1-piperazinocarbonyl chloride over a period of 10 minutes. During this period, the pH of the solution was maintained at 11.0 to 12.0 by gradual addition of a 10% aqueous sodium hydroxide solution. The solution was reacted at said temperature for 1 hour, and the temperature thereof was elevated to 5.degree. to 10.degree. C., after which the mixture was further reacted at said temperature for 2 hours, while maintaining the pH thereof at 10.0 to 11.0 by addition of a 10% aqueous sodium hydroxide solution. After the reaction, tetrahydrofuran was removed by distillation under reduced pressure, and the residue was dissolved in a mixed solvent comprising 20 ml of water and 50 ml of ethyl acetate. The resulting solution was adjusted to a pH of 1.5 by addition of dilute hydrochloric acid with ice-cooling, and then the organic layer was separated off. The aqueous layer was further extracted with 50 ml of ethyl acetate, and the resulting organic layer was combined with the aforesaid organic layer. The combined organic layer was washed with water and then dried over anhydrous magnesium sulfate. To this organic layer was added 0.9 g of a sodium salt of 2-ethylhexonic acid to deposit white crystals. The deposited crystals were collected by filtration and then dried to obtain 1.26 g of white crystals of a sodium salt of D(-)-.alpha.-(2-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetic acid, m.p. 215.degree. C. (decomp.), yield 70%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1650-1590.

(2) To a suspension in 15 ml of anhydrous acetone of 1.0 g of the above-mentioned sodium salt of D(-)-.alpha.-(2-methyl-3-oxo-1-piperazinocarbonylamino)-phenylacetic acid was added 10 mg of N-methylmorpholine. The resulting mixture was cooled to -20.degree. to -15.degree. C., and a solution of 380 mg of ethyl chlorocarbonate in 5 ml of anhydrous acetone was dropped into said mixture over a period of 5 minutes. Subsequently, the mixture was stirred at said temperature for 60 minutes, and then cooled to -40.degree. to -30.degree. C. Into the thus treated mixture was dropped a solution of 960 mg of a triethylamine salt of 6-aminopenicillanic acid in 10 ml of anhydrous methylene chloride over a period of 10 minutes. Thereafter, the mixture was reacted with stirring at -30.degree. to -20.degree. C. for 60 minutes, at -20.degree. to -10.degree. C. for 30 minutes, and at -10.degree. to -0.degree. C. for 30 minutes. After the reaction, the organic solvent was removed by distillation under reduced pressure. The residue was dissolved in a mixed solvent comprising 20 ml of water and 50 ml of ethyl acetate, and the resulting solution was adjusted to a pH of 1.5 by addition of dilute hydrochloric acid with ice-cooling. Subsequently, the organic layer was separated off, sufficiently washed with water and then dried over anhydrous magnesium sulfate. To this organic layer was added 0.5 g of a sodium salt of 2-ethylhexonic acid with ice-cooling to deposit white crystals. The deposited crystals were collected by filtration, and then dried to obtain 1.39 g of a sodium salt of 6-[D(-)-.alpha.-(2-methyl-3-oxo-1-piperazinocarbonylamino)-phenylacetamido]penicillanic acid, m.p. 208.degree. C. (decomp.), yield 90%.

In the same manner as above, 2.0 g of a sodium salt of 6-[D(-)-.alpha.-(4-ethyl-3-oxo-1-piperazinocarbonylamino)propionamido]penicillanic acid, m.p. 195.degree. C. (decomp.), yield 86%, was obtained from 1.59 g of a sodium salt of D(-)-.alpha.-(4-ethyl-3-oxo-1piperazinocarbonylamino)propionic acid and 1.59 g of a triethylamine salt of 6-aminopenicillanic acid.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1760 (lactam), 1680-1600 (--CON<, --COO.sup..crclbar.).

EXAMPLE 6

(1) Into a solution of 0.5 g of phosgene in 10 ml of anhydrous dioxane was dropped at 10.degree. C. 10 ml of anhydrous dioxane containing 0.56 g of 1-allyl-2-oxo-piperazine and 0.5 g of triethylamine, upon which reaction took place to deposit white crystals of triethylamine hydrochloride. Subsequently, the deposited crystals were collected by filtration, and the filtrate was concentrated to dryness to obtain 800 mg of pale yellow, oily 4-allyl-3-oxo-1-piperazinocarbonyl chloride.

IR (film) cm.sup.-1 : .nu..sub.C=O 1720, 1640.

(2) A suspension of 1.4 g of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by gradual addition of triethylamine with stirring, and then cooled to 0.degree. C. Into the thus treated suspension was dropped 10 ml of a tetrahydrofuran solution containing 800 mg of the aforesaid 4-allyl-3-oxo-1-piperazinocarbonyl chloride. During this period, the pH of the suspension was maintained at 7.5 to 8.5 by gradual addition of triethylamine. Subsequently, the resulting mixture was reacted at said temperature for 30 minutes, and the temperature thereof was then elevated to 10.degree. to 15.degree. C., after which the mixture was further reacted at said temperature for 90 minutes while maintaining the pH thereof at 7.5 to 8.0 by addition of triethylamine. After the reaction, the tetrahydrofuran was removed by distillation under reduced pressure, and the residue was dissolved in 20 ml of water. The resulting solution was washed with ethyl acetate, and the aqueous layer was then separated off. This aqueous layer was ice-cooled and adjusted to a pH of 1.5 by addition of dilute hydrochloric acid to deposit white crystals. The deposited crystals were collected by filtration, sufficiently washed with water and then dried to obtain 1.8 g of 6-[D(-)-.alpha.-(4-allyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 92.degree. C. (decomp.), yield 90%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1760 (lactum), 1720-1620 (--COOH, --CON<).

The above-mentioned operation was repeated, except that the 4-allyl-3-oxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 11, to obtain respective objective compounds as shown in Table 11. The structure of each objective compound was confirmed by IR and NMR.

TABLE 11__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ ##STR352## ##STR353## ##STR354## ##STR355## ##STR356## ##STR357## ##STR358## ##STR359## ##STR360## ##STR361## ##STR362## ##STR363## ##STR364## ##STR365## ##STR366## ##STR367## ##STR368## ##STR369## ##STR370## ##STR371##__________________________________________________________________________

EXAMPLE 7

Using 0.63 g of 6-[D(-)-.alpha.-aminophenylacetamido]pencillanic acid and 600 mg of a hydrochloride of 4-(N-morpholinomethyl)-3-oxo-1-piperazinocarbonyl chloride, the same operation as in Example 6 was repeated to obtain 0.63 g of 6-{D(-)-.alpha.-[4-(N-morpholinomethyl)-3-oxo-1-piperazinocarbonylamino]-phenylacetamido}penicillanic acid, m.p. 85.degree. C. (decomp.), yield 60%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O : 1770 (lactam), 1600-1680 (--COO.sup..crclbar., --CON<).

EXAMPLE 8

Using 5.0 g of a hydrochloride of pivaloyloxymethyl ester of 6-[D(-)-.alpha.-aminophenylacetamido]-penicillanic acid and 1.94 g of 2-methyl-3-oxo-1-piperazinocarbonyl chloride, the same operation as in Example 6 was repeated to obtain 5.2 g of a pivaloyloxymethyl ester of 6-[D(-)-.alpha.-(2-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-penicillanic acid, m.p. 140.degree. C. (decomp.), yield 80%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1740-1770 (lactam, ester) 1630-1670 (--CON<).

EXAMPLE 9

(1) Into a mixture comprising 8.0 g of 4-acetyl-2,5-dioxo-piperazine, 5.0 g of triethylamine and 100 ml of anhydrous tetrahydrofuran was dropped 6.0 g of trimethylchlorosilane with stirring at room temperature. After the dropping, the resulting mixture was reacted at said temperature for 2 hours to deposit triethylamine hydrochloride. The deposited hydrochloride was separated by filtration, and the filtrate was dropped at 0.degree. to 5.degree. C. into 100 ml of an anhydrous tetrahydrofuran solution containing 10.0 g of phosgene. After completion of the dropping, the resulting mixture was stirred at 10.degree. to 15.degree. C. for 3 hours to terminate the reaction. Subsequently, the tetrahydrofuran and the excess phosgene were removed by distillation under reduced pressure to obtain 11.0 g of oily 4-acetyl-2,5-dioxo-1-piperazinocarbonyl chloride.

(2) A suspension of 17.5 g of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 200 ml of tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by gradual addition of triethylamine with stirring at 10.degree. to 15.degree. C. to form a homogeneous solution. Into this solution was dropped a solution of 11.0 g of the aforesaid 4-acetyl-2,5-dioxo-1-piperazinocarbonyl chloride in 30 ml of tetrahydrofuran at 0.degree. C. over a period of 30 minutes. During this period, the pH of the reaction solution was maintained at 7.5 to 8.0 by gradual addition of triethylamine. Subsequently, the temperature of the resulting mixed solution was elevated to 5.degree. to 10.degree. C. and the solution was further reacted for 1 hour while maintaining the pH thereof at 7.5 to 8.0 by addition of triethylamine. After completion of the reaction, the tetrahydrofuran was removed by distillation under reduced pressure. To the residue was added 100 cc of N hydrochloric acid at 0.degree. to 10.degree. C., and the resulting mixture was stirred for 30 minutes to deposit white crystals. The deposited crystals were collected by filtration, and again suspended in water. The resulting aqueous suspension was adjusted to a pH of 8.0 by gradual addition of triethylamine at 5.degree. to 10.degree. C., and then freed from insolubles by filtration. The filtrate was adjusted to a pH of 1.5 by gradual addition of N hydrochloric acid to deposit crystals. The deposited crystals were collected by filtration, washed with water and then dried to obtain 21.2 g of 6-[D(-)-.alpha.-(4-acetyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 162.degree.-164.degree. C. (decomp.), yield 80%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770 (lactam), 1730-1660 (--COOH, --CON<).

NMR ((CD.sub.3).sub.2 CO).tau. values: 0.23 (1H), 2.65 (5H), 4.26 (1H), 4.33-4.63 (2H), 5.38 (4H), 5.68 (1H), 7.55 (3H), 8.47 (3H), 8.53 (3H).

The above-mentioned operation was repeated, except that the 4-acetyl-2,5-dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 12, to obtain respective objective compounds as shown in Table 12. The structure of each objective compound was confirmed by IR and NMR.

TABLE 12__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ D(-)- ##STR372## ##STR373## m.p. (decomp.) 88.degree. C., yield 60% D(-)- ##STR374## ##STR375## m.p. (decomp.) 179-181.degree. C., yield 83% D(-)- ##STR376## ##STR377## m.p. (decomp.) 88.degree. C., yield 82% D(-)- ##STR378## ##STR379## m.p. 214-215.degree. C., yield 89.6% D(-)- ##STR380## ##STR381## m.p. (decomp.) 176-181.degree. C., yield 84.4% D(-)- ##STR382## ##STR383## m.p. (decomp.) 148-151.degree. C., yield 92% D(-)- ##STR384## ##STR385## m.p. (decomp.) 95-100.degree. C., yield 91% D(-)- ##STR386## ##STR387## m.p. (decomp.) 120-125.degree. C., yield 92% D(-)- ##STR388## ##STR389## m.p. (decomp.) 147-149.degree. C., yield__________________________________________________________________________ 89%

EXAMPLE 10

(1) A suspension of 8.0 g of D(-)-.alpha.-aminophenyl acetic acid in 80 ml of tetrahydrofuran was adjusted to a pH of 11.5 by gradual addition of a N sodium hydroxide solution with stirring to form a homogeneous solution. This solution was cooled to 0.degree. C., and 15 ml of a tetrahydrofuran solution containing 11 g of 4-acetyl-2,5-dioxo-1-piperazinocarbonyl chloride was dropped at said temperature into said solution over a period of 30 minutes. During this period, the pH of the reaction solution was maintained at 10.5 to 11.0 by gradual addition of a N sodium hydroxide solution. Subsequently, the temperature of the resulting mixed solution was elevated to 5.degree. to 10.degree. C., and the mixture was further reacted for 1 hour, upon which D(-)-.alpha.-aminophenylacetic acid deposited. After completion of the reaction, the deposited acid was separated by filtration, and the filtrate was concentrated under reduced pressure to remove tetrahydrofuran. The residue was dissolved in a mixed solvent comprising 10 ml of water and 80 ml of ethyl acetate, and the resulting solution was adjusted to a pH of 1.0 by addition of dilute hydrochloric acid with ice-cooling. Subsequently, the organic layer was separated off, dried over anhydrous magnesium sulfate, and then charged into 100 ml of an ethyl acetate solution containing 8.3 g of sodium 2-ethylhexonate to deposit crystals. The deposited crystals were collected by filtration, washed with acetone, and then dried over P.sub.2 O.sub.5 to obtain 7.9 g of a sodium salt of D(-)-.alpha.-(4-acetyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid, m.p. 104.degree. C. (decomp.), yield 42%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1690-1650, 1600-1590.

(2) To a suspension in 25 ml of anhydrous acetone of 1.75 g of the aforesaid sodium salt of D(-)-.alpha.-(4-acetyl-2,5-dioxo-1-piperazinocarbonylamino)phenyl acetic acid was added 20 mg of N-methylmorpholine, and the resulting mixture was cooled to -20.degree. to -15.degree. C. Into this mixture was dropped a solution of 0.57 g of ethyl chlorocarbonate in 5 ml of anhydrous acetone over a period of 5 minutes, and the mixture was stirred at said temperature for 60 minutes. Subsequently, a solution of 1.29 g of a triethylamine salt of 6-aminopenicillanic acid in 30 ml of anhydrous methylene chloride was dropped into said mixture at -40.degree. to -30.degree. C. over a period of 10 minutes. The temperature of the resulting mixture was elevated from -30.degree. C. to 0.degree. C., and the mixture was then reacted at said temperature for about 2 hours. After the reaction, the solvent was removed by distillation under reduced pressure. The residue was charged into 30 ml of water, and the resulting mixture was freed from insolubles by filtration with ice-cooling. The filtrate was adjustable to a pH of 1.5 to 2.0 by addition of dilute hydrochloric acid to deposit crystals. The deposited crystals were collected by filtration, sufficiently washed with water, and then dried to obtain 2.34 g of 6-[D(-)-.alpha.-(4-acetyl-2,5-dioxo-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 162.degree.-164.degree. C. (decomp.), yield 90%.

In the same manner as above, 530 mg of 6-[D(-)-.alpha.-(4-benzyl-2,2-pentamethylene-3,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 95.degree.-100.degree. C., yield 82.68%, was obtained from 450 mg of D(-)-.alpha.-(4-benzyl-2,2-pentamethylene-3,5-dioxo-1-piperazinocarbonylamino)phenylacetic acid and 320 mg of a triethylamine salt of 6-aminopenicillanic acid.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770 (lactam), 1700-1660 (--COOH, --CON<).

EXAMPLE 11

(1) Into a mixture comprising 8 g of a diethyl ester of oxalic acid and 8 ml of ethanol was dropped at room temperature 4.4 g of N-ethyl ethylenediamine. The resulting mixture was allowed to react for 3 hours, and then heated to remove the ethanol. Subsequently, the residue was recrystallized from 10 ml of dioxane to obtain 5.4 g of 1-ethyl-2,3-dioxo-piperazine, m.p. 124.degree. C., yield 76.0%.

(2) To a suspension of 0.71 g of the above-mentioned 1-ethyl-2,3-dioxo-piperazine in 15 ml of anhydrous dioxane were added with stirring 0.70 g of trimethylsilyl chloride and 0.83 ml of triethylamine. The resulting mixture was stirred at room temperature for 20 hours to deposit triethylamine hydrochloride. This hydrochloride was separated by filtration, and the filtrate was dropped at 5.degree. to 10.degree. C. into a solution of 0.70 g of phosgene in 10 ml of anhydrous tetrahydrofuran. Subsequently, the resulting mixture was reacted at 5.degree. to 10.degree. C. for 30 minutes and at room temperature for 2 hours, and then the solvent was removed by distillation under reduced pressure to obtain 1.0 g of pale yellow crystals of 4-ethyl-2,3-dioxo-1-piperazinocarbonyl chloride.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780, 1660.

(3) A suspension of 1.75 g of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 50 ml of tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by addition of triethylamine with stirring to form a solution. This solution was cooled to 0.degree. to 5.degree. C., and then 7 ml of an anhydrous tetrahydrofuran solution containing 1.0 g of the aforesaid 4-ethyl-2,3-dioxo-1-piperazinocarbonyl chloride was dropped into the solution. During this period, the pH of the reaction solution was maintained at 7.5 to 8.0 by gradual addition of triethylamine. The resulting mixed solution was reacted at said temperature for 30 minutes and then at 5.degree. to 10.degree. C. for 1 hour, while maintaining the pH thereof at 7.5 to 8.0. After the reaction, the tetrahydrofuran was removed by distillation under reduced pressure, and the residue was dissolved in 20 ml of water and then washed two times with 20 ml of ethyl acetate. To the aqueous layer was again added 50 ml of ethyl acetate, and the resulting mixture was adjusted to a pH of 1.5 by gradual addition of dilute hydrochloric acid with ice-cooling. Subsequently, the ethyl acetate layer was separated off, sufficiently washed with water, and then dried over anhydrous magnesium sulfate. Into the thus treated layer was dropped 10 ml of an ethyl acetate solution containing 0.83 g of sodium 2-ethylhexonate to deposit white crystals. The deposited crystals were collected by filtration, sufficiently washed with ethyl acetate, washed with diethyl ether, and then dried to obtain 2.4 g of a sodium salt of 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 183.degree.-185.degree. C. (decomp.), yield 89%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1765 (lactam), 1720-1670 (--CON<), 1600 (--COO.sup..crclbar.).

NMR ((CD.sub.3).sub.2 SO+D.sub.2 O) .tau. values: 2.62 (5H), 4.31 (1H), 4.50 (1H), 4.70 (1H), 6.05 (1H), 6.35-6.65 (6H), 8.49 (3H), 8.60 (3H), 8.91 (3H).

The above-mentioned operation was repeated, except that the 4-ethyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 13, to obtain respective objective compounds as shown in Table 13. The structure of each objective compound was confirmed by IR and NMR.

TABLE 13__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ ##STR390## ##STR391## ##STR392## ##STR393## ##STR394## ##STR395## ##STR396## ##STR397## ##STR398## ##STR399## ##STR400## ##STR401## ##STR402## ##STR403## ##STR404## ##STR405## ##STR406## ##STR407## ##STR408## ##STR409##__________________________________________________________________________

EXAMPLE 12

A suspension of 1.4 g of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 30 ml of tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by addition of triethylamine with stirring to form a solution. This solution was cooled to 0.degree. to 5.degree. C., and 10 ml of a tetrahydrofuran solution containing 1.2 g of 4-n-pentyl-2,3-dioxo-1-piperazinocarbonyl chloride was dropped into said solution. During this period, the pH of the reaction solution was maintained at 7.5 to 8.5 by gradual addition of triethylamine. Subsequently, the resulting mixed solution was reacted at said temperature for 30 minutes and then at 10.degree. to 15.degree. C. for 90 minutes, while maintaining the pH thereof at 7.5 to 8.5. After the reaction, the tetrahydrofuran was removed by distillation under reduced pressure, and the residue was dissolved in 20 ml of water and then washed two times with 20 ml of ethyl acetate. To the aqueous layer was further added 30 ml of ethyl acetate, and the resulting mixture was adjusted to a pH of 1.5 by addition of dilute hydrochloric acid with ice-cooling. Thereafter, the ethyl acetate layer was separated off, sufficiently washed with water, dried over magnesium sulfate, and then freed from the solvent by distillation under reduced pressure. The residue was crystallized by addition of diisopropyl ester to obtain 1.8 g of crystals of 6-[D(-)-.alpha.-(4-n-pentyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 96.degree. C. (decomp.), yield 80.5%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770 (lactam), 1720-1660 (--CON<, --COOH).

NMR ((CD.sub.3).sub.2 SO+D.sub.2 O).tau. values: 2.62 (5H), 4.31 (1H), 4.51-4.69 (2H), 6.04 (1H), 6.20-6.90 (6H), 8.50 (3H). 8.60 (3H), 8.75 (6H), 8.90 (3H).

The above-mentioned operation was repeated, except that the 4-n-pentyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 14, to obtain respective objective compounds as shown in Table 14. The structure of each objective compound was confirmed by IR and NMR.

TABLE 14__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ D(-)- ##STR410## ##STR411## m.p. (decomp.) 107.degree. C., yield 89% D(-)- ##STR412## ##STR413## m.p. (decomp.) 92.degree. C., yield 88.5% D(-)- ##STR414## ##STR415## m.p. (decomp.) 95.degree. C., yield 79.8% D(-)- ##STR416## ##STR417## m.p. (decomp.) 80-82.degree. C., yield__________________________________________________________________________ 95%

EXAMPLE 13

Using 1.7 g of a triethylamine salt of 6-[D(-)-.alpha.-amino-p-hydroxyphenylacetamido]penicillanic acid and 0.7 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride, the same operation as in Example 12 was repeated to obtain 1.2 g of a sodium salt of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]penicillanic acid, m.p. 170.degree.-172.degree. C. (decomp.), yield 75%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1760 (lactam), 1710-1660 (--CON<), 1600 (--COO.crclbar.).

NMR ((CD.sub.3).sub.2 SO) .tau.values: 2.8-3.3 (4H), 4.45 (1H), 4.65 (2H), 6.05 (1H), 6.2 (4H), 6.97 (3H), 8.48 (3H), 8.60 (3H).

In the same manner as above, a sodium salt of 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]penicillanic acid, m.p. 175.degree. C. (decomp.), yield 72%, was obtained from 4-ethyl-2,3-dioxo-1-piperazinocarbonyl chloride and a triethylamine salt of 6-[D(-)-.alpha.-amino-p-hydroxyphenylacetamido]penicillanic acid.

EAMPLE 14

To a solution of 0.8 g of a phthalide ester of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 10 ml of tetrahydrofuran was added 0.25 ml of triethylamine. Into the resulting mixture was dropped 0.32 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride with ice-cooling, and the mixture was reacted at room temperature for 2 hours. After the reaction, the solvent was removed by distillation under reduced pressure. The residue was dissolved in a mixed solvent comprising 20 ml of ethyl acetate and 20 ml of water, and the resulting solution was adjusted to a pH of 2 by addition of dilute hydrochloric acid. Subsequently, the organic layer was separated off, washed with water, washed with a 2% aqueous sodium hydrogencarbonate solution, washed with water, dried over magnesium sulfate, and then concentrated to a liquid amount of about 2 ml. To the concentrate was added 20 ml of diisopropyl ether to deposit crystals, which were then collected to obtain 0.95 g of crystals of a phthalide ester of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 157.degree.-160.degree. C. (decomp.), yield 90.0%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1715 (ester), 1680 (--CON<).

NMR (CD.sub.3l ).sub.2 CO+D.sub.2 O) .tau.values: 2.12 (4H), 2.40 (1H), 2.58 (5H), 4.25-4.60 (3H), 5.45 (1H), 5.85-6.42 (4H), 6.90 (3H), 8.50 (6H).

The above-mentioned operation was repeated, except that the 4-methyl-2,3dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 15, to obtain respective objective compounds as shown in Table 15. The structure of each objective compound was confirmed by IR and NMR.

TABLE 15__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ ##STR418## ##STR419## ##STR420## ##STR421## ##STR422## ##STR423##

A solution of 0.86 g of a hydrochloride of methoxymethyl ester of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 15 ml of tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by addition of triethylamine at 0.degree. to 5.degree. C. Into this solution, a solution of 0.38 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride in 10 ml of tetrahydrofuran was dropped over a period of 10 minutes. During this period, the pH of the reaction solution was maintained at 7.5 to 8.0 by gradual addition of triethylamine. The resulting mixed solution was reacted for 30 minutes, while maintaining the pH thereof at 7.5 to 8.0. After completion of the reaction, the tetrahydrofuran was removed by distillation under reduced pressure. The residue was dissolved in a mixed solvent comprising 50 ml of water and 50 ml of ethyl acetate, and the resulting solution was adjusted to a pH of 1.5 by addition of dilute hydrochloric acid with ice-cooling. Subsequently, the organic layer was separated off, washed with water, dried over anhydrous magnesium sulfate, and then freed from the solvent by distillation under reduced pressure to form crystals. The thus formed crystals were washed with diethyl ether to obtain 0.9 g of a methoxymethyl ester of 6-[D(-)-.alpha.-(4methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 111.degree.-115.degree. C. (decomp.), yield 82.5%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1740 (ester), 1700-1660 (--CON<).

NMR ((CD.sub.3).sub.2 CO) .tau. values: 0.15 (1H), 2.0 (1H), 2.67 (5H), 4.3-4.5 (3H), 4.75 (2H), 5.7 (1H), 6.55 (4H), 6.97 (3H), 7.25 (3H), 8.84 (3H), 8.60 (3H).

The above-mentioned operation was repeated, except that the 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 16, to obtain respective objective compounds as shown in Table 16. The structure of each objective compound was confirmed by IR and NMR.

TABLE 16__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ D(-)- ##STR424## ##STR425## m.p. (decomp.) 83-85.degree. C., yield 80.2% D(-)- ##STR426## ##STR427## m.p. (decomp.) 78-80.degree. C., yield 80% D(-)- ##STR428## ##STR429## m.p. (decomp.) 93-95.degree. C., yield 82.5% D(-)- ##STR430## ##STR431## m.p. (decomp.) 70-74.degree. C., yield__________________________________________________________________________ 74.4%

EXAMPLE 16

Using 1.5 g of a hydrochloride of pivaloyloxymethyl ester of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid and 0.6 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride, the same operation as in Example 15 was repeated to obtain a pivaloyloxymethyl ester of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 108.degree.-111.degree. C. (decomp.), yield 75%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1750 (ester), 1710-1660 (--CON<).

The above-mentioned operation was repeated, except that the 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 17, to obtain respective objective compounds as shown in Table 17. The structure of each objective compound was confirmed by IR and NMR.

TABLE 17__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ ##STR432## ##STR433## ##STR434## ##STR435##__________________________________________________________________________

EXAMPLE 17

Using 0.81 g of a hydrochloride of 62-piperidinoethyl ester of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid and 0.3 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride, the same operation as in Example 15 was repeated to obtain 0.75 g of a .beta.-piperidinoethyl ester of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 166.degree.-169.degree. C. (decomp.), yield 78%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1740 (ester), 1710-1670 (--CON<).

NMR (CDCl.sub.3) .tau. values: 2.7 (5H), 4.3-4.6 (3H), 5.7 (1H), 5.75 (2H), 6.0 (2H), 6.4 (2H), 6.9 (3H), 7.45 (2H), 7.6 (4H), 8.5 (12H).

The above-mentioned operation was repeated, except that the 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by 4-n-octyl-2,3-dioxo-1-piperazinocarbonyl chloride, to obtain a .beta.-piperidinoethyl ester of 6-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 110.degree.-115.degree. C. (decomp.), yield 73.58%.

EXAMPLE 18

Using 0.93 g of a hydrochloride of .beta.-morpholinoethyl ester of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid and 0.39 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride, the same operation as in Example 15 was repeated to obtain 0.8 g of a .beta.-morpholinoethyl ester of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 150.degree.-153.degree. C. (decomp.), yield 73%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1740 (ester), 1710-1680 (--CON<).

NMR (CDCl.sub.3) .tau. values: 2.55 (5H), 4.3-4.55 (3H), 5.6 (1H), 5.7 (3H), 6.0 (2H), 6.3 (2H), 7.4 (2H), 7.5 (4H), 8.5 (6H).

The above-mentioned operation was repeated, except that the 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by 4-n-octyl-2,3-dioxo-1-piperazinocarbonyl chloride, to obtain a .beta.-morpholinoethyl ester of 6-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 103.degree.-105.degree. C. (decomp.), yield 70%.

EXAMPLE 19

(1) To a solution of 8.7 g of a sodium salt of D(-)-.alpha.-phenylglycine in 50 ml of water were added 50 ml of ethyl acetate and 5.05 g of triethylamine. To the resulting mixture was gradually added 9.5 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride at 0.degree. to 5.degree. C. over a period of 15 minutes, and then the mixture was reacted at 5.degree. to 15.degree. C. for 30 minutes. After the reaction, the aqueous layer was separated off, washed with diethyl ether, and then adjusted to a pH of 1.5 by addition of dilute hydrochloric acid to deposit crystals. The deposited crystals were collected by filtration, washed with water and dried to obtain 14.1 g of D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid, m.p. 138.degree.-141.degree. C. (decomp.), yield 87%. Recrystallization from hydrous butanol gave white crystals, m.p. 140.degree.-142.degree. C. (decomp.).

Elementary analysis (for C.sub.14 H.sub.15 N.sub.3 O.sub.5.H.sub.2 O): Calculated (%)--C: 52.01, H: 5.30, N: 13.00; Found (%)--C: 52.24, H: 5.32, N: 12.87.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1710, 1700, 1660.

(2) Into a solution of 10 g of the above-mentioned D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid in 200 ml of acetone was dropped a solution of 5.2 g of a sodium salt of 2-ethylhexonic acid in 50 ml of acetone with stirring to deposit crystals. The deposited crystals were collected by filtration and then washed with acetone to obtain 9.6 g of a sodium salt of D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid, m.p. 165.degree. C. (decomp.), yield 95%.

(3) To a suspension of 8.8 g of the above-mentioned sodium salt of D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperzinocarbonylamino) phenylacetic acid in 80 ml of methylene chloride was added 20 mg of N-methylmorpholine. Into the resulting mixture was dropped a solution of 3.1 g of ethyl chlorocarbonate in 20 ml of methylene chloride at -20.degree. to -15.degree. C. over a period of 5 minutes, and the mixture was reacted at said temperature for 1 hour. Into this reaction liquid was dropped a solution of 9.4 g of a triethylamine salt of 6-aminopenicillanic acid in 40 ml of methylene chloride at -40.degree. to -30.degree. C. over a period of 10 minutes, and the resulting mixture was reacted at -40.degree. to -20.degree. C. over a period of 1 hour. After the reaction, the temperature of the reaction liquid was gradually elevated at 0.degree. C. over a period of 1 hour, and the mixture was then subjected to extraction with 100 ml of water. Subsequently, the aqueous layer was separated off, and the methylene chloride layer was further subjected to extraction with 50 ml of water, and the resulting aqueous layer was combined with the aforesaid aqueous layer. The combined aqueous layer was adjusted to a pH of 2 addition of dilute hydrochloric acid with ice-cooling to deposit crystals. The deposited crystals were collected by filtration, sufficiently washed with water, dried and then dissolved in 200 ml of acetone. Into the resulting solution was dropped a solution of 4 g of a sodium salt of 2-ethylhexonic acid in 40 ml of acetone over a period of 10 minutes to deposite crystals. The deposited crystals were collected by filtration, washed with acetone and then dried to obtain 11.4 g of a sodium salt of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 170.degree. C. (decomp.), yield 80.8%.

The above-mentioned operation was repeated, except that the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid was replaced by each of the compounds of formula (V) shown in Table 18, to obtain respective objective compounds as shown in Table 18. The structure of each objective compound was confirmed by IR and NMR.

TABLE 18__________________________________________________________________________Compound of formula (V) Objective compound__________________________________________________________________________ ##STR436## ##STR437## ##STR438## ##STR439## ##STR440## ##STR441##__________________________________________________________________________

EXAMPLE 20

(1) To a solution of 2.28 g of D(-)-.alpha.-amino-1,4-cyclohexadienylacetic acid in 15 ml of N NaOH were added 20 ml of ethyl acetate and 2.1 ml of triethylamine, and the resulting mixture was cooled to 0.degree. C. To this mixture was gradually added 1.69 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride over a period of 10 minutes. Subsequently, the mixture was reacted over 30 minutes with ice-cooling, and then the aqueous layer was separated off. To the aqueous layer was further added 20 ml of ethyl acetate. The resulting mixture was adjusted to a pH of 2 by addition of 2 N hydrochloric acid with ice-cooling, and the ethyl acetate layer was separated off. The organic layer was sufficiently washed with water, dried over anhydrous magnesium sulfate, freed from the solvent by distillation under reduced pressure and then incorporated with isopropyl alcohol to deposit crystals. The deposited crystals were collected by filtration to obtain 2.5 g of white crystals of D(-)-.alpha.-(4-methyl- 2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetic acid, m.p. 140.degree.-145.degree. C. (decomp.), yield 74%.

IR (KBr) cm.sup.-1 : .nu..sub.NH 3300, .nu..sub.C=O 1715, 1660.

NMR (d.sub.6 -DMSO) .tau. values: 0.57 (1H, d), 4.26 (1H, s), 4.36 (2H, s), 5.29 (1H, d), 6.07-6.18 (2H, m), 6.38-6.49 (2H, m), 7.05 (3H, s), 7.35 (4H, s).

(2) To a suspension of 0.45 g of the above-mentioned D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetic acid in 15 ml of anhydrous methylene chloride was added 0.24 ml of N-methylmorpholine with stirring to form a solution. After cooling the solution of -10.degree. C., 3 ml of an anhydrous methylene chloride solution containing 0.24 g of ethyl chlorocarbonate was dropped into the solution, and the resulting mixture was reacted at said temperature for 90 minutes. Subsequently, the reaction liquid was cooled to -20.degree. C., and 5 ml of a methylene chloride solution containing 0.70 g of a triethylamine salt of 6-aminopenicillanic acid and 0.31 of triethylamine was gradually dropped into the reaction liquid. The resulting mixture was reacted at -20.degree. C. for 1 hour, at -20.degree. to 0.degree. C. for 1 hour, and at 0.degree. to 5.degree. C. for 1 hour. Thereafter, the reaction liquid was freed from the solvent by distillation under reduced pressure. The residue was dissolved in 10 ml of water and then washed with 10 ml of ethyl acetate. The aqueous layer was again incorporated with 15 ml of ethyl acetate, and then adjusted to a pH of 2.0 by addition of 2 N HCl with ice-cooling. Subsequently, the ethyl acetate layer was separated off, washed with water, dried over anhydrous magnesium sulfate, and freed from the solvent by distillation under reduced pressure to obtain 0.74 g of white crystals of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetamido]penicillanic acid, m.p. 84.degree.-87.degree. C. (decomp.), yield 87%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1730-1660 (--COOH, --CON<).

NMR (d.sub.6 -DMSO) .tau. values: 0.55 (1H, d), 0.95 (1H, d), 4.22 (1H, s), 4.35 (2H, s), 4.41-4.61 (2H, s), 4.92 (1H, d), 5.75 (1H, s), 6.05 (2H, bs), 6.40 (2H, bs), 7.03 (3H, s), 7.35 (4H, s), 8.40 (3H, s), 8.52 (3H, s).

The thus obtained product was adjusted to a pH of 7.0 by neutralization with an aqueous sodium hydrogencarbonate solution, and then subjected to filtration and freeze-drying to obtain a sodium salt thereof.

The above-mentioned operation was repeated, except that the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-1,4-cyclohexadienylacetic acid was replaced by each of the compounds of formula (V) shown in Table 19, to obtain respective objective compounds as shown in Table 19. The structure of each objective compound was confirmed by IR and NMR.

TABLE 19__________________________________________________________________________Compound of formula (V) Objective compound__________________________________________________________________________ ##STR442## ##STR443## ##STR444## ##STR445## ##STR446## ##STR447##__________________________________________________________________________

EXAMPLE 21

(1) To a solution of 2.2 g of DL-.alpha.-amino-2-thienylacetic acid in 14 ml of a N sodium hydroxide solution was added at 0.degree. C. 2.2 g of triethylamine. To the resulting mixture was further added 3.6 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride little by little at said temperature. Subsequently, the mixture was reacted at 0.degree. C. for 30 minutes, and then at room temperature for 30 minutes. After the reaction, the reaction liquid was adjusted to a pH of 1.0 by addition of dilute hydrochloric acid to deposit crystals. The deposited crystals were collected by filtration, washed with water and then dried to obtain 3.5 g of DL-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid, m.p. 214.degree.-215.degree. C. (decomp.), yield 80.5%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1710, 1680-1660.

(2) Into a solution of 3.5 g of the above-mentioned DL-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid in 100 ml of acetone was dropped a solution of 1.86 g of a sodium salt of 2-ethylhexonic acid in 50 ml of acetone, upon which crystals were deposited. The deposited crystals were collected by filtration and then washed with acetone to obtain 3.5 g of a sodium salt of DL-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid, m.p. 175.degree.-176.degree. C. (decomp.).

(3) To a suspension of 3.3 g of the above-mentioned sodium salt of DL-.alpha.-(4-methyl-2,3dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid in 50 ml of methylene chloride was added 30 mg of N-methylmorpholine, and the resulting mixture was then cooled to -20.degree. to -15.degree. C. Into the resulting mixture was dropped a solution of 1.3 g of ethyl chlorocarbonate in 20 ml of methylene chloride over a period of 5 minutes, and the mixture was stirred at said temperature for 90 minutes. Subsequently, a solution of 3.3 g of a triethylamine salt of 6-aminopenicillanic acid in 50 ml of methylene chloride was dropped into the mixture at -50.degree. to -40.degree. C. over a period of 20 minutes, and the resulting mixture was reacted with stirring at -40.degree. to -30.degree. C. for 30 minutes, at -30.degree. to -20.degree. C. for 30 minutes, and then at -20.degree. to 0.degree. C. for 30 minutes. After the reaction, the solvent was removed by distillation under reduced pressure, and the residue was dissolved in water. The resulting aqueous solution was adjusted to a pH of 2.0 by addition of dilute hydrochloric acid with ice-cooling to deposit crystals. The deposited crystals were collected by filtration, sufficiently washed with water and then dried to obtain 4.1 g of 6-[DL-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetamido]penicillanic acid, m.p. 185.degree. C. (decomp.), yield 80.5%.

IR (nujol) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1715 (--COOH), 1685-1675 (--CON<).

NMR ((CD.sub.3).sub.2 CO) .tau. values: 0.5 (1H), 1.8 (1H), 2.6 (1H), 2.85-3.05 (2H), 4.0 (1H), 4.2-4.5 (2H), 5.7 (1H), 5.8-6.0 (2H), 6.2-6.4 (2H), 6.95 (3H), 8.4 (3H), 8.45 (3H).

The thus obtained product was adjusted to a pH of 7.0 by neutralization with an aqueous sodium hydrogencarbonate solution, and then subjected to filtration and freeze-drying to obtain a sodium salt thereof.

The above-mentioned operation was repeated, except that the sodium salt of DL-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-2-thienylacetic acid was replaced by each of the compounds of formula (V) shown in Table 20, to obtain respective objective compounds as shown in Table 20. The structure of each objective compound was confirmed by IR and NMR.

TABLE 20__________________________________________________________________________Compound of formula (V) Objective compound__________________________________________________________________________ ##STR448## ##STR449## ##STR450## ##STR451## ##STR452## ##STR453##__________________________________________________________________________

EXAMPLE 22

To a suspension of 0.9 g of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 30 ml of anhydrous ethyl acetate were added at 5.degree. to 10.degree. C. 0.55 g of triethylamine and 0.6 g of trimethylsilyl chloride. The resulting mixture was reacted at 15.degree. to 20.degree. C. for 3 hours to form trimethylsilylated 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid. To this acid was then added 1 g of 4-ethyl-2,3-dioxo-1-piperazinocarbonyl chloride, and the resulting mixture was reacted at 15.degree. to 20.degree. C. for 2 hours. After the reaction, a deposited triethylamine hydrochloride was separated by filtration, and the filtrate was incorporated with 0.4 g of n-butanol to deposit crystals. The deposited crystals were collected by filtration to obtain 1.25 g of white crystals of 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid. Into a solution of said crystals in 30 ml of tetrahydrofuran was dropped a solution of 0.38 g of a sodium salt of 2-ethylhexonic acid in 10 ml of tetrahydrofuran, upon which white crystals were deposited. The deposited crystals were collected by filtration, sufficiently washed with tetrahydrofuran and then dried to obtain 1.25 g of a sodium salt of 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 183.degree.-185.degree. C. (decomp.), yield 90%.

EXAMPLE 23

To a suspension of 4 of a trihydrate of 6-[D(-)-.alpha.-aminophenylacetamido]penicillanic acid in 40 ml of water was added 20 ml of ethyl acetate, and the resulting mixture was cooled to 2.degree. C. Subsequently, the mixture was incorporated with 1.37 g of potassium carbonate, and then stirred at 2.degree. to 3.degree. C. for 2 minutes. Thereafter, 1.89 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride was added to the mixture at said temperature over a period of 10 minutes, and the resulting mixture was reacted at said temperature for 15 minutes. After the reaction, slight amounts of insolubles were separated by filtration, and the filtrate was charged into 80 ml of ethyl acetate. Into the resulting mixture was dropped 5 ml of 2 N HCl at 20.degree. to 22.degree. C. over a period of 5 minutes, and the mixture was stirred at said temperature for 5 hours to deposit crystals. The deposited crystals were collected by filtration, washed two times with 4 ml of water, further washed two times with 4 ml of isopropanol, and then dried to obtain 4.0 g of a dihydrate of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 156.degree.-157.degree. C. (decomp.), yield 75.4%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1775, 1740, 1695, 1670.

NMR (d.sub.6 -DMSO) .tau. values: 0.18 (1H, d), 0.77 (1H, d), 2.66 (5H, s), 4.30 (1H, d), 4.40 (3H, br), 4.48 (1H, g), 4.65 (1H, d), 5.80 (1H, s), 6.12 (2H, bs), 6.45 (2H, bs), 7.06 (3H, s), 8.48 (3H, s), 8.60 (3H, s).

The above-mentioned operation was repeated, except that the 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by 4-ethyl-2,3-dioxo-1-piperazinocarbonyl chloride, to obtain a monohydrate of 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 154.degree.-156.degree. C. (decomp.), yield 84.8%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1775, 1735, 1705, 1680, 1665.

NMR (d.sub.6 -DMSO) .tau. values: 0.20 (1H, d), 0.76 (1H, d), 2.69 (5H, s), 4.32 (1H, d), 4.53 (1H, q), 4.64 (1H, d), 5.00 (3H, br), 5.83 (1H, s), 6.13 (2H, bs), 6.49 (2H, bs), 6.62 (2H, q), 8.44 (3H, s), 8.58 (3H, s), 8.91 (3H, t).

The thus obtained monohydrate was neutralized with an aqueous sodium hydrogencarbonate solution, and then subjected to filtration and freeze-drying to obtain a sodium salt of 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid.

Further, a solution in 10 ml of nitromethane of 2 g of the aforesaid dihydrate of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid was allowed to stand overnight to deposit crystals, which were then collected by filtration to obtain 2 g of a monohydrate of a nitromethane addition product of 6-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 128.degree.-130.degree. C. (decomp.), yield 92.2%.

Elementary analysis (for C.sub.22 H.sub.25 N.sub.5 O.sub.7 S.CH.sub.3 NO.sub.2.H.sub.2 O): Calculated (%)--C: 47.42, H: 5.19, N: 14.43; Found (%)--C: 47.94, H; 5.13, N: 14.53.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770, 1735, 1700, 1680.

NMR (d.sub.6 -DMSO) .tau. values: 0.22 (1H, d), 0.80 (1H, d), 2.69 (5H, s), 3.30 (3H, br), 4.30 (1H, d), 4.46-4.70 (2H), 5.67 (3H, s), 5.81 (1H, s), 6.13 (2H, bs), 6.46 (2H, bs), 7.07 (3H, s), 8.45 (3H, s), 8.58 (3H, s).

EXAMPLE 24

To a suspension of 1.6 g of a trihydrate of D(-)-.alpha.-aminobenzyl penicillin in 20 ml of water was added at 2.degree. to 3.degree. C. 0.54 g of potassium carbonate, and the resulting mixture was stirred for 3 minutes. To the mixture was gradually added 0.81 g of 4-ethyl-2,3-dioxo-1-piperazinocarbonyl chloride at said temperature over a period of 10 minutes, and the mixture was reacted for 15 minutes. After the reaction, slight amounts of insolubles formed were separated by filtration, and the filtrate was charged into 10 ml of methyl n-propyl ketone. Into the resulting mixture was dropped 1.98 ml of 2 N HCl at 15.degree. to 20.degree. C. over a period of 2 minutes, and the mixture was stirred at said temperature for 1 hour to deposit crystals. The deposited crystals were collected by filtration, washed two times with 2 ml of water, further washed two times with 2 ml of methyl n-propyl ketone, and then dried to obtain 1.7 g of a monohydrate of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)benzylpenicillin, m.p. 152.degree.-154.degree. C. (decomp.), yield 80.2%.

The thus obtained product was neutralized with an aqueous sodium hydrogencarbonate solution, and then subjected to filtration and freeze-drying to obtain a sodium salt of the said product.

EXAMPLE 25

A suspension of 4.0 g of a monohydrate of 7-[D(-)-.alpha.-aminophenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid in 60 ml of tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by gradual addition of triethylamine with stirring to form a solution, which was then cooled to 0.degree. C. To this solution were gradually added 2.5 g of crystals of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride over a period of 10 minutes. During this period, the pH of the reaction solution was maintained at 7.5 to 8.0 by gradual addition of triethylamine. Subsequently, the resulting mixture was reacted at 0.degree. to 5.degree. C. for 15 minutes while maintaining the pH thereof at 7.5 to 8.0. After the reaction, the reaction liquid was stirred together with 60 ml of diethyl ether and 70 ml of water, and then the aqueous layer was separated off. The thus obtained aqueous layer was washed with 30 ml of ethyl acetate, cooled to 0.degree. to 5.degree. C., and then adjusted to a pH of 1.5 by addition of dilute hydrochloric acid to deposit white crystals. The deposited crystals were collected by filtration, sufficiently washed with water and then dried to obtain 4.7 g of white crystals of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 185.degree.-186.degree. C. (decomp.), yield 86%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770-1760 (lactam), 1720-1660 (--CON<, --COOH).

NMR (d.sub.6 -DMSO) .tau. values: 0.1 (1H, d), 0.56 (1H, d), 2.62 (5H, s), 4.26-4.37 (2H, dd), 5.05 (1H, d), 6.1 (2H, bs), 6.47 (2H, bs), 6.63 (2H, s), 7.05 (3H, s), 8.02 (3H, s).

The above-mentioned operation was repeated, except that the 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 21, to obtain respective objective compounds as shown in Table 21. The structure of each objective compound was confirmed by IR and NMR.

TABLE 21__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ ##STR454## ##STR455## ##STR456## ##STR457## ##STR458## ##STR459##__________________________________________________________________________

EXAMPLE 26

(1) To a solution of 0.92 g of 1-n-pentyl-2,3-dioxo-piperazine in 15 ml of anhydrous dioxane were added 1.1 ml of triethylamine and 1.08 g of trimethylsilyl chloride. The resulting mixture was stirred at room temperature for 20 hours to form triethylamine hydrochoride. This hydrochloride was separated by filtration, and the filtrate was dropped at 0.degree. to 5.degree. C. into a solution of 0.6 g of phosgene in 10 ml of anhydrous tetrahydrofuran. Subsequently, the resulting mixture was reacted at 5.degree. to 10.degree. C. for 30 minutes and then at room temperature for 2 hours. Thereaafter, the solvent was removed by distillation under reduced pressure to obtain 1.21 g of pale yellow, oily 4-n-pentyl-2,3-dioxo-1-piperazinocarbonyl chloride.

IR (film) cm.sup.-1 : .nu..sub.C=O 1790, 1720-1665.

(2) A suspension of 1.70 g of a monohydrate of 7-[D(-)-.alpha.-aminophenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid in 50 ml of tetrahydrofuran containing 20% by volume of water was adjusted to a pH of 8.0 to 8.5 by addition of triethylamine with stirring to form a solution. This solution was cooled to 0.degree. to 5.degree. C., and 7 ml of an anhydrous tetrahydrofuran solution containing 1.21 g of the 4-n-pentyl-2,3-dioxo-1-piperazinocarbonyl chloride obtained in (1) was dropped into the solution. During this period, the pH of the solution was maintained at a pH of 7.5 to 8.0 by addition of triethylamine. Subsequently, the resulting mixed solution was reacted at 0.degree. to 5.degree. C. for 1 hour and then at 5.degree. to 10.degree. C. for 2 hours while maintaining the pH thereof at 7.5 to 8.0. After the reaction, the tetrahydrofuran was removed by distillation under reduced pressure, and the residue was dissolved in 20 ml of water and then washed two times with 20 ml of ethyl acetate. The aqueous layer was again charged with 40 ml of ethyl acetate, and then adjusted to a pH of 1.5 by gradual addition of dilute hydrochloric acid with ice-cooling. Subsequently, the ethyl acetate layer was separated off, washed with water, and then dried over anhydrous magnesium sulfate. Thereafter, 10 ml of an ethyl acetate solution containing 0.75 g of sodium 2-ethylhexonate was dropped into the layer at 0.degree. to 5.degree. C. to deposite white crystals. The deposited crystals were collected by filtration, and washed with ethyl acetate and then with diethyl ether to obtain 1.95 g of a sodium salt of 7-[D(-)-.alpha.-(4-n-pentyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 164.degree.-166.degree. C. (decomp.), yield 75 %.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1750 (lactam), 1720-1660 (--CON<), 1590 (--COO.sup..crclbar.).

NMR (d.sub.6 -DMSO+D.sub.2 O) .tau. values: 2.58 (5H, s), 4.33 (1H, s), 4.49 (1H, d), 5.17 (1H, d), 6.10 (2H, bs), 6.42-6.87 (6H, m), 8.09 (3H, s), 8.60-8.90 (6H, bs), 9.12 (3H, t).

The above-mentioned operation was repeated, except that the 4-n-pentyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 22, to obtain respective objective compounds as shown in Table 22. The structure of each objective compound was confirmed by IR and NMR.

TABLE 22__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ D(-)- ##STR460## ##STR461## m.p. (decomp.) 160.degree. C., yield 77.7% D(-)- ##STR462## ##STR463## m.p. (decomp.) 158.degree. C., yield 78% D(-)- ##STR464## ##STR465## m.p. (decomp.) 154.degree. C., yield 78% D(-)- ##STR466## ##STR467## m.p. (decomp.) 185-188.degree. C., yield 77% D(-)- ##STR468## ##STR469## m.p. (decomp.) 135-137.degree. C., yield__________________________________________________________________________ 79.2%

EXAMPLE 27

Using 1.5 g of a hydrochloride of methoxymethyl ester of 7-[D(-)-.alpha.-aminophenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid and 0.65 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride, the same operation as in Example 25 was repeated to obtain 1.6 g of a methoxymethyl ester of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 146.degree.-148.degree. C. (decomp.,), yield 86%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770 (lactam), 1710 (ester), 1680-1600 (--CON<).

EXAMPLE 28

To a suspension of 0.20 g of 7-[D(-)-.alpha.-aminophenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid in 15 ml of anhydrous chloroform was added 0.17 ml of triethylamine with stirring to form a solution, which was then cooled to 0.degree. C. To this solution was added 0.11 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride, and the resulting mixture was reacted at room temperature for 2 hours. After the reaction, the reaction liquid was evaporated under reduced pressure, and the residue was dissolved in 15 ml of water. The resulting solution was washed with 10 ml of ethyl acetate. The aqueous layer was again charged with 20 ml of ethyl acetate, and then adjusted to a pH of 1.5 by addition of 2N hydrochloric acid with ice-cooling. Subsequently, the ethyl acetate layer was separated off, successively washed with water and a saturated aqueous sodium chloride solution, and then dried over magnesium sulfate. Thereafter, the solvent was removed by distillation under reduced pressure to obtain 0.22 g of white crystals of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 175.degree. C. (decomp.), yield 76%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770 (lactam), 1720-1650 (--CON<, --COOH).

NMR (d.sub.6 -DMSO) .tau. values: 0.23 (1H, d), 0.63 (1H, d), 2.66 (5H, s), 4.32 (1H, q), 4.43 (1H, d), 5.05 (1H, d), 5.21 (2H, q), 6.15 (2H, bs), 6.40 (2H, bs), 6.57 (2H, bs), 7.0 (3H, s), 8.0 (3H, s).

The above-mentioned operation was repeated, except that the 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 23, to obtain respective objective compounds as shown in Table 23. The structure of each objective compound was confirmed by IR and NMR.

TABLE 23__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________ D(-)- ##STR470## ##STR471## m.p. (decomp.) 150.degree. C., yield 83.4% D(-)- ##STR472## ##STR473## m.p. (decomp.) 165.degree. C., yield 83% D(-)- ##STR474## ##STR475## m.p. (decomp.) 146.degree. C., yield 82% D(-)- ##STR476## ##STR477## m.p. (decomp.) 112.degree. C., yield 95% D(-)- ##STR478## ##STR479## m.p. (decomp.) 134.degree. C., yield 90.2%__________________________________________________________________________

The aforesaid 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 175.degree. C. (decomp.) was recrystallized from hydrous acetone to obtain white crystals showing a melting point of 198.degree. to 200.degree. C. (decomp.)

EXAMPLE 29

(1) To a solution of 28.2 g of a sodium salt of D(-)-phenylglycine in 150 ml of water were added 200 ml of ethyl acetate and 18.2 g of triethylamine, and the resulting mixture was cooled to 0.degree. C. To this mixture was added 34.3 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride over a period of 15 minutes, and the mixture was reacted at 5.degree. to 10.degree. C. for 15 minutes. Thereafter, the aqueous layer was separated off and adjusted to a pH of 0.5 by addition of 2N hydrochloric acid with ice-cooling to deposit crystals. The deposited crystals were collected by filtration and then dried to obtain 42 g of white crystals of D(-)-.alpha.(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid, m.p. 195.degree. C. (decomp.)

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1700, 1660.

NMR (d.sub.6 -DMSO) .tau. values: 0.1 (1H, d), 2.65 (5H, s), 4.60 (1H, d), 6.10 (2H, bs), 6.50 (2H, bs), 7.0 (3H, s).

(2) To a suspension in 15 ml of anhydrous methylene chloride of 0.31 g of the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid obtained in the above-mentioned item (1) was added 0.11 g of N-methylmorpholine with stirring to form a solution, which was then cooled to -20.degree. C. To this solution was added 3 ml of an anhydrous methylene chloride solution containing 0.13 g of ethyl chlorocarbonate, and the resulting mixture was reacted at -10.degree. to 20.degree. C. for 60 minutes to form a mixed acid anhydride. Into the thus formed acid anhydride was dropped a solution formed by adding 0.50 ml of triethylamine to a suspension in 5 ml of methanol of 0.41 g of 7-amino-3-[2-(5-methyl-1,3,4-thiadiazoly)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid. After the dropping, the resulting mixture was reacted at -50.degree. to -30.degree. C. for 30 minutes, at -30.degree. to -20.degree. C. for 30 minutes, at -20.degree. to 0.degree. C. for 60 minutes, and then at room temperature for 30 minutes. Thereafter, the reaction liquid was concentrated under reduced pressure, and the concentrate was dissolved in 10 ml of water, washed with 5 ml of ethyl acetate, again charged with 15 ml of ethyl acetate, and then adjusted to a pH of 1.5 by addition of 2N hydrochloric acid with ice-cooling. Subsequently, insolubles were separated by filtration, and the ethyl acetate layer was separated off, successively washed with water and a saturated sodium chloride solution, dried over magnesium sulfate, and then freed from the solvent by distillation under reduced pressure to obtain 0.58 g of pale yellow crystals of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 160.degree. C. (decomp.), yield 91%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1650-1720 (--CON<, --COOH).

NMR (d.sub.6 -DMSO) .tau. values: 0.2 (1H, d), 0.6 (1H, d), 2.60 (5H, s), 4.35 (1H, q), 4.40 (1H, d), 5.0 (1H, d), 5.70 (2H, q), 6.10 (2H, bs), 6.25-6.55 (2H, 2H, bs), 7.0 (3H, s), 7.30 (3H, s).

The above-mentioned operation was repeated, except that the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid was replaced by each of the compounds of formula (V) shown in Table 24, to obtain respective objective compounds as shown in Table 24. The structure of each objective compound was confirmed by IR and NMR.

TABLE 24__________________________________________________________________________Compound of formula (V) Objective compound__________________________________________________________________________D(-)- D(-)- ##STR480## ##STR481## m.p. (decomp.) 150.degree. C., yield 91%D(-)- D(-)- ##STR482## ##STR483## m.p. (decomp.) 147.degree. C., yield 85.4%D(-)- D(-)- ##STR484## ##STR485## m.p. (decomp.) 144.degree. C., yield 84.3%D(-)- D(-)- ##STR486## ##STR487## m.p. (decomp.) 167.degree. C., yield__________________________________________________________________________ 93%

EXAMPLE 30

Using 0.3 g of D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid and 0.33 g of 7-amino-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, the same operation as in Example 29 was repeated, to obtain 0.5 g of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 161.degree.-163.degree. C. (decomp.), yield 76%.

IR (nujol) cm.sup.-1 : .nu..sub.C=O 1775 (lactam), 1720-1660 (--CON<, --COOH).

NMR (d.sub.6 -DMSO) .tau. values: 0.02 (1H, d), 0.34 (1H, d), 2.48 (5H, s), 4.17 (1H, q), 4.26 (1H, d), 4.92 (1H, d), 5.66 (2H, s), 6.01 (5H, s), 6.35 (4H, s), 7.0 (3H, s).

The above-mentioned operation was repeated, except that the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid was replaced by each of the compounds of formula (V) shown in Table 25, to obtain respective objective compounds as shown in Table 25. The structure of each objective compound was confirmed by IR and NMR.

TABLE 25__________________________________________________________________________Compound of formula (V) Objective compound__________________________________________________________________________ ##STR488## ##STR489## ##STR490## ##STR491## ##STR492## ##STR493##__________________________________________________________________________ *Anhydrous methylene chloride was substituted for the methanol used in Example 29.

EXAMPLE 31

Using 0.30 g of D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid and 0.34 g of 7-amino-3-[5-(1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, the same operation as in Example 29 was repeated, to obtain 0.47 g of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,3,4-thiadiazolyl)thiomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 158.degree.-159.degree. C. (decomp.), yield 71.5 %.

IR (nujol) cm.sup.-1 : .nu..sub.C=O 1775 (lactam), 1720-1660 (--CON<, --COOH).

The above-mentioned operation was repeated, except that the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid was replaced by D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid, to obtain 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 123.degree. C. (decomp.), yield 64.5%.

EXAMPLE 32

Using 0.31 g of D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid and 0.39 g of 7-amino-3-[2-(1-methyl-1,3,4-triazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, the same operation as in Example 29 was repeated, except that the methanol was replaced by anhydrous methylene chloride, to obtain 0.43 g of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, yield 70%.

IR (nujol) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1720-1650 (--CON<, --COOH).

The above-mentioned operation was repeated, except that the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid was replaced by each of the compounds of formula (V) shown in Table 26, to obtain respective objective compounds as shown in Table 26. The structure of each objective compound was confirmed by IR and NMR.

TABLE 26__________________________________________________________________________Compound of formula (V) Objective compound__________________________________________________________________________ ##STR494## ##STR495## ##STR496## ##STR497##__________________________________________________________________________

EXAMPLE 33

The procedure of Example 29 was repeated, except that the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid was replaced by each of the compounds of formula (V) shown in Table 27, to obtain respective objective compounds shown in Table 27. The structure of each objective compound was confirmed by IR and NMR.

TABLE 27__________________________________________________________________________Compound of formula (V) Objective compound__________________________________________________________________________D(-)- D(-)- ##STR498## ##STR499##D(-)- D(-)- ##STR500## ##STR501##D(-)- D(-)- ##STR502## ##STR503##D(-)- D(-)- ##STR504## ##STR505##D(-)- D(-)- ##STR506## ##STR507##D(-)- D(-)- ##STR508## ##STR509##D(-)- D(-)- ##STR510## ##STR511##D(-)- D(-)- ##STR512## ##STR513##D(-)- D(-)- ##STR514## ##STR515##__________________________________________________________________________

EXAMPLE 34

The procedure of Example 30 was repeated, except that the D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid was replaced by each of the compounds of formula (V) shown in Table 28, to obtain respective objective compounds shown in Table 28. The structure of each objective compound was confirmed by IR and NMR.

TABLE 28__________________________________________________________________________Compound of formula (V) Objective compound__________________________________________________________________________D(-)- D(-)- ##STR516## ##STR517##D(-)- D(-)- ##STR518## ##STR519##D(-)- D(-)- ##STR520## ##STR521##D(-)- D(-)- ##STR522## ##STR523##D(-)- D(-)- ##STR524## ##STR525##D(-)- D(-)- ##STR526## ##STR527##D(-)- D(-)- ##STR528## ##STR529##D(-)- D(-)- ##STR530## ##STR531##D(-)- D(-)- ##STR532## ##STR533##__________________________________________________________________________

EXAMPLE 35

(1) To a suspension of 0.9 g of D(-)-.alpha.-alanine in 15 ml of water was added 2.05 g of triethylamine to dissolve D(-)-.alpha.-alanine in water, and the resulting solution was cooled to 0.degree. C. To the solution was added 2.3 g of 4-methyl-2,3-dioxo-1-piperazinocarbonyl chloride over 15 minutes, after which reaction was effected for 30 minutes with ice-cooling. Dilute hydrochloric acid was then added to the reaction product to adjust the pH thereof to 2.0. The water was removed by distillation under reduced pressure, and 30 ml of acetone was added to the residue, after which insolubles were filtered off. To the resulting acetone solution was added 10 ml of an acetone solution of 1.6 g of a sodium salt of 2-ethylhexonic acid, and the deposited crystals were collected by filtration, and dried to obtain 2.1 g of a sodium salt of D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)propionic acid having a melting point of 115.degree.-8.degree. C. (decomp.), yield 78.5%.

IR (KBr) cm.sup.-1 :.nu..sub.C=O 1700, 1680, 1600 (--CON<, --COO.sup.63).

(2) In the same manner as in Example 32, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)propionamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid was obtained from a sodium salt of D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)propionic acid and 7-amino-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid. The thus obtained product was dissolved in 20 ml of acetone, and a solution of 0.65 g of a sodium salt of 2-ethylhexonic acid in 5 ml of acetone was added to the resulting solution. The deposited crystals were collected by filtration and dried to obtain 1.2 g of sodium salt of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)propionamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid having a melting point of 195.degree. C. (decomp.), yield 67.7%.

IR (KBr) cm.sup.-1 ; .nu..sub.C=O 1780 (lactam), 1710-1660 (--CON-), 1600 (--COO.sup..crclbar.).

EXAMPLE 36

In the same manner as in Example 32, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonyl)-p-hydroxyphenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid was obtained from 7-amino-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid and D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetic acid.

Melting point (decomp.), 147.degree.-9.degree. C.; yield, 62.0%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1765 (lactam), 1720-1660 (--CON<, --COOH).

In the same manner as above, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 188.degree.-190.degree. C. (decomp.), yield 80.7%, was obtained from 7-amino-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid and D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetic acid.

EXAMPLE 37

In the same manner as in Example 29, 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-azidomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid was obtained from D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid and 7-amino-3-azidomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid.

Melting point (decomp.), 185.degree.-8.degree. C.; yield, 68.0%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1775 (lactam), 1720-1660 (--CON<, --COOH); .nu..sub.N.sbsb.3 2090.

EXAMPLE 38

In 10 ml of a phosphoric acid buffer solution of a pH of 6.3 was suspended 0.57 g of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, and 0.07 g of sodium hydrogencarbonate was dissolved therein. To the solution was then added 0.12 g of 1-methyl-5-mercapto-1,2,3,4-tetrazole to dissolve the latter in the former, and the solution was subjected to reaction for 24 hours while maintaining the pH of the solution at 6.5-6.7 by using dilute hydrochloric acid and sodium hydrogencarbonate. After the reaction, the reaction liquid was cooled, and then adjusted to a pH of 5.0 by adding dilute hydrochloric acid. The reaction liquid was sufficiently washed with ethyl acetate, after which the aqueous layer was separated off and then adjusted to a pH of 1.5 by adding dilute hydrochloric acid thereto. The deposited crystals were collected by filtration and dried, after which the dried crystals were washed with ethyl acetate to obtain 0.40 g of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 163.degree.-165.degree. C. (decomp.), yield 74.5%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1775 (lactam), 1720-1660 (--CON<, --COOH).

NMR (d.sub.6 -DMSO) .tau. values: 0.18 (1H, d), 0.55 (1H, d), 2.64 (5H, s), 4.3 (1H, q), 4.4 (1H, d), 5.0 (1H, d), 5.75 (2H, s), 6.05 (5H, s), 6.3-6.8 (6H), 8.92 (3H, t).

In the same manner as above, the objective compounds shown in Table 29 were obtained from 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonyl)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid or 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid and the compounds of formula (VII) shown in Table 29. All the objective compounds were D(-) isomers, and the structure of each objective compound was confirmed by IR and NMR.

TABLE 29__________________________________________________________________________Compound offormula (VII) Objective compound__________________________________________________________________________ ##STR534## ##STR535## ##STR536## ##STR537## ##STR538## ##STR539## ##STR540## ##STR541## ##STR542## ##STR543## ##STR544## ##STR545## ##STR546## ##STR547## ##STR548## ##STR549## ##STR550## ##STR551## ##STR552## ##STR553## ##STR554## ##STR555## ##STR556## ##STR557## ##STR558## ##STR559## ##STR560## ##STR561## ##STR562## ##STR563##NaN.sub.3 ##STR564## ##STR565## ##STR566## ##STR567## ##STR568## ##STR569## ##STR570##__________________________________________________________________________

EXAMPLE 39

In 10 ml of water was suspended 1.15 g of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, and 0.17 g of sodium hydrogencarbonate was then dissolved therein, after which 0.48 g of pyridine and 4.1 g of potassium thiocyanate were added thereto. The resulting mixture was subjected to reaction at 60.degree. C. for 5 hours while maintaining the pH of the mixture at 6.0 to 6.5 by adding dilute hydrochloric acid or sodium hydrogencarbonate. After the reaction, 20 ml of water was added to dilute the reaction mixture, which was then sufficiently washed with chloroform. The aqueous layer was then separated off and then adjusted to a pH of 1.5 by adding dilute hydrochloric acid. The deposited crystals were collected by filtration, dried, and then washed with acetone to obtain 1.04 g (yield, 79.6%) of a thiocyanic acid salt of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-pyridinomethyl-.DELTA..sup.3 -cephem- 4-carboxylic acid betaine having a melting point (decomp.) of 155.degree.-160.degree. C., said product having the formula, ##STR571##

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1720-1660 (--CON<); .nu.SCN 2040.

In the same manner as above, a thiocyanic acid salt of 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-pyridinomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid betaine was obtained from 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethy-1-.DELTA..sup.3 -cephem-4-carboxylic acid and pyridine, said product having the formula, ##STR572##

Melting point (decomp.), 180.degree.-185.degree. C.; yield, 82.0%.

In a conventional manner, the above two products were treated with an ion exchange resin to obtain the desired 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-pyridinomethyl-.DELTA..sup.3 -cephem-4 -carboxylic acid betaine and 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-pyridinomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid betaine.

EXAMPLE 40

In 85 ml of anhydrous methanol was dissolved 1.5 g of a sodium salt of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(pyridyl-1-oxide)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid. To the resulting solution was added 0.65 g of anhydrous cupric chloride, and the resulting mixture was stirred at room temperature for 15 minutes and then subjected to reaction at 50.degree. C. for 14 hours. After the reaction, hydrogen sulfide gas was passed through the reaction solution with ice-cooling for 20 minutes. The resulting insolubles were filtered off, and the filtrate was concentrated under reduced pressure. To the residue was added 20 ml of a 5% aqueous sodium hydrogen-carbonate solution, and the insolubles were filtered off, after which dilute hydrochloric acid was added to the filtrate to adjust the pH to 6.5. The filtrate was then washed with 10-ml portions of ethyl acetate three times, after which the aqueous layer was separated off and then adjusted to a pH of 1.8 by adding dilute hydrochloric acid thereto. The thus deposited crystals were collected by filtration and then dried under reduced pressure and washed with 20 ml of an ethyl acetate-chloroform mixed solvent (1:1 by volume) to obtain 0.40 g of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 162.degree.-6.degree. C. (decomp.), yield 30.5 %.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770 (lactam), 1700 (--COOH), 1666 (--CON<).

NMR (d.sub.6 -DMSO) .tau. values: 0.13 (1H, d), 0.53 (1H, d), 2.61 (5H, s), 4.31 (1H, q), 4.41 (1H, d), 4.96 (1H, d), 5.82 (2H, s), 6.10 (2H bs), 6.33 (2H, 2H, 2H, bs), 6.79 (3H, s), 8.89 (3H, t).

EXAMPLE 41

To a solution of 3.2 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid in 20 ml of anhydrous methylene chloride and 5 ml of dimethylformamide was added 1.33 g of N,N-dimethylaniline. The resulting mixture was cooled to -15.degree. to -10.degree. C., and a solution of 1.14 g of ethyl chlorocarbonate in 5 ml of anhydrous methylene chloride was dropped into said mixture over a period of 5 minutes. The mixture was reacted at said temperature for 60 minutes.

On the other hand, to a suspension of 3.28 g of 7-amino-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid in 65 ml of anhydrous acetonitrile was added 3.04 g of N,O-bis(trimethylsilyl)acetamide to obtain a solution. The solution was cooled to -20.degree. C. and poured into the aforesaid reaction mixture. Subsequently, the mixture was reacted at -10.degree. C. to -5.degree. C. for 60 minutes, and at 5.degree. to 10.degree. C. for 60 minutes. After the reaction, to the reaction mixture 5 ml of methanol was added and the mixture was freed from insolubles by filtration. Thereafter the solvent was removed by distillation under reduced pressure. The residue was dissolved in a mixed solvent comprising 100 ml of water and 50 ml of ethyl acetate, and the resulting solution was adjusted to a pH of 7.5 to 8.0 by addition of sodium hydrogencarbonate, after which the aqueous layer was added 80 ml of ethyl acetate and 20 ml of acetone, and the resulting solution was adjusted to a pH of 1.5 by addition of dilute hydrochloric acid. Subsequently, the organic layer was separated off, sufficiently washed with water and then the solvent was removed from ethyl acetate layer by distillation under reduced pressure. The residue was dissolved in 15 ml of acetone, and to this solution was added 60 ml of 2-propanol with stirring to deposit white crystals. The deposited crystals were collected by filtration, sufficiently washed with 2-propanol and then dried to obtain 5.26 g of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 163.degree.-165.degree. C. (decomp.), yield 83.6%. The structure of this compound was confirmed by IR and NMR.

The above-mentioned operation was repeated except that the sodium salt of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid was replaced by each of the reactive derivatives of compounds of formula (III) shown in Table 30, to obtain the respective objective compounds as shown in Table 30. The structure of each objective compound was confirmed by IR and NMR.

TABLE 30__________________________________________________________________________Reactive derivative ofcompound of formula (III) Objective compound__________________________________________________________________________D(-)- D(-)- ##STR573## ##STR574## m.p. (decomp.) 161-163.degree. C., yield 85.8%D(-)- D(-)- ##STR575## ##STR576## m.p. (decomp.) 188-190.degree. C., yield 75.5%D(-)- D(-)- ##STR577## ##STR578## m.p. (decomp.) 162-165.degree. C., yield 74.0%D(-)- D(-)- ##STR579## ##STR580## m.p. (decomp.) 124-128.degree. C., yield__________________________________________________________________________ 75.3%

EXAMPLE 42

Using 3.1 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid and 3.0 g of 7-amino-3-[5-(1,2,3-triazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, the same operation as in Example 41 was repeated to obtain 4.5 g of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,2,3-triazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 177.degree.-180.degree. C. (decomp.), yield 76.7%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770 (lactom), 1703 (--COOH), 1680, 1667 (--CON<).

EXAMPLE 43

Using 1.5 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetic acid and 1.5 g of 7-amino-3-[5-(2-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, the same operation as in Example 41 was repeated to obtain 2.3 g of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]-3-[5-(2-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 172.degree.-177.degree. C. (decomp.), yield 77.7%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1780 (lactam), 1710 (--COOH), 1685, 1672 (--CON<).

In the same manner as above, the objective compounds shown in Table 31 were obtained from D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid or D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetic acid and the compounds of formula (IV) shown in Table 31. The structure of each objective compound was confirmed by IR and NMR.

TABLE 31__________________________________________________________________________Compound of formula (IV) Objective compound__________________________________________________________________________ D(-)- ##STR581## ##STR582## m.p. (decomp.) 171-175.degree. C., yield 72.5% D(-)- ##STR583## ##STR584## m.p. (decomp.) 156-160.degree. C., yield 73.1% D(-)- ##STR585## ##STR586## m.p. (decomp.) 163-165.degree. C., yield 67.4% D(-)- ##STR587## ##STR588## m.p. (decomp.) 159-161.degree. C., yield 68.0% D(-)- ##STR589## ##STR590## m.p. (decomp.) 183-185.degree. C., yield 77.7% D(-)- ##STR591## ##STR592## m.p. (decomp.) 167.degree. C., yield 82.0% D(-)- ##STR593## ##STR594## m.p. (decomp.) 168-174.degree. C., yield__________________________________________________________________________ 65.0%

EXAMPLE 44

In a mixed solvent comprising 80 ml of water and 40 ml of ethyl acetate was suspended 4.0 g of 7-[D(-)-.alpha.-aminophenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, and 1.65 g of anhydrous potassium carbonate was then dissolved therein with ice-cooling.

To this solution was added 7.3 g of 4-(.alpha.-hydroxyethyl)-2,3-dioxo-1-piperazinocarbonyl chloride at 0.degree. to 5.degree. C. over a period of 15 minutes, and the resulting mixture was reacted at 10.degree. to 15.degree. C. for 30 minutes. After the reaction, the aqueous layer was separated off, 100 ml of acetonitrile was added to the aqueous layer, and the resulting solution was adjusted to a pH of 1.5 by addition of dilute hydrochloric acid, and was saturated by sodium chloride. Subsequently, the acetonitrile layer was separated off, successively washed two times with 30 ml of saturated solution of sodium chloride and then dried by reduced pressure. The residue was dissolved in a mixed solvent comprising 50 ml of acetone and 10 ml of ethanol, and insolubles were separated off. Subsequently, 100 ml of isopropyl alcohol was added to the resulting solution, and then concentrated to a liquid amount of about two-thirds under reduced pressure. The deposited crystals were collected by filtration, washed with iso-propyl alcohol and then dried to obtain 4.5 g of 7-[D(-)-.alpha.-4-(.beta.-hydroxyethyl)-2,3-dioxo-1-piperazinocarbonylaminophenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 142.degree.-144.degree. C. (decomp.), yield 76.3%.

IR (KBr) cm.sup.-1 : .nu..sub.C=O 1770 (lactam), 1708 (--COOH), 1680, 1665 (--CON<).

The above-mentioned procedure was repeated, except that the 7-[D(-)-.alpha.-aminophenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid was replaced by each of the compounds of formula (II) shown in Table 32, to obtain the respective objective compounds as shown in Table 32. The structure of each objective compound was confirmed by IR and NMR.

TABLE 32__________________________________________________________________________Compound of formula (II) Objective compound__________________________________________________________________________ D(-)- ##STR595## ##STR596## m.p. (decomp.) 153-157.degree. C., yield 62.0% D(-)- ##STR597## ##STR598## m.p. (decomp.) 141-145.degree. C., yield 64.6% D(-)- ##STR599## ##STR600## m.p. (decomp.) 170-173.degree. C., yield__________________________________________________________________________ 58.0%

EXAMPLE 45

(1) To a solution of 0.63 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid in 10 ml of anhydrous methylene chloride were added 0.5 g of oxalyl chloride and a drop of N,N-dimethylformamide, and the resulting mixture was reacted at room temperature for 30 minutes. After the reaction, the solvent was removed by distillation under reduced pressure. The residue was washed with anhydrous benzene to obtain 0.6 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetyl chloride, m.p. 112.degree.-116.degree. C. (decomp.), yield 88.8%.

IR (KBr) cm.sup.-1 : .nu..sub.NH 3280; .nu..sub.C=O 1790, 1695.

(2) To a suspension of 0.27 g of 7-aminocephalosporanic acid in 6 ml of anhydrous methanol was added 0.24 g of triethylamine. The resulting solution was cooled to -40.degree. C., and to the solution was added a solution of 5 ml of anhydrous methylene chloride containing 0.34 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetyl chloride, and then the temperature of the reaction liquid was gradually elevated to room temperature over a period of 1 hour. After the reaction, the solvent was removed by distillation under reduced pressure. The residue was added to 20 ml of water, and the resulting solution was washed two times with 5 ml of ethyl acetate. To the aqueous layer was added 20 ml of ethyl acetate, and the solution was adjusted to a pH of 1.5 by addition of 2N hydrochloric acid with stirring. Subsequently, the organic layer was separated off, washed with saturated aqueous sodium chloride, dried over anhydrous magnesium sulfate, and then the solvent was removed by distillation under reduced pressure to obtain 453 mg of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 165.degree.-166.degree. C. (decomp.), yield 79.0%.

In the same manner as above, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 168.degree.-174.degree. C. (decomp.), yield 72.3%, was obtained from D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonaylamino)-p-hydroxyphenylacetyl chloride and 7-aminocephalosporanic acid.

EXAMPLE 46

To a suspension of 2.16 g of 6-aminopenicillanic acid in 20 ml of methylene chloride was added 2.02 g of triethylamine. Into the resulting solution was added 3.4 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetyl chloride at 10.degree. to 15.degree. C. and the resulting mixture was reacted at 10.degree. to 15.degree. C. for 2 hours. After the reaction, the solvent was removed by distillation under reduced pressure. The residue was added to 20 ml of water, and to the resulting solution was added 30 ml of ethyl acetate. The solution was adjusted to a pH of 2.5 by addition of 2N hydrochloric acid and was stirred over a period of 4 hours to deposit crystals. The deposited crystals were collected by filtration to obtain 4.4 g of 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]penicillanic acid, m.p. 154.degree.-156.degree. C. (decomp.), yield 82.2%.

In the same manner as above, 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]penicillanic acid m.p. 160.degree.-161.degree. C. (decomp.), yield 70.5%, was obtained from 6-aminopenicillanic acid and D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetyl chloride.

EXAMPLE 47

Using D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid and 7-amino-3-carbamoyl-oxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, the same procedure as in Example 41 was repeated, to obtain 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-carbamoyloxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 175.degree.-180.degree. C. (decomp.), yield 68.0%.

IR (KBr) cm.sup.-1 : .nu..sub.NH.sbsb.2 3450, 3350; .nu..sub.CONH 3300; .nu..sub.C=O 1778, 1710, 1670.

In the same manner as above, 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]-3-carbamoyloxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 178.degree.-182.degree. C. (decomp.), yield 65.0%, was obtained from D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetic acid and 7-amino-3-carbamoyloxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid.

EXAMPLE 48

(1) To a solution of 0.32 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid in 15 ml of methylene chloride was added 0.1 g of N-methylmorpholine. The resulting solution was cooled to -20.degree. C., and to the solution was added a solution of 0.11 g of ethyl chlorocarbonate in 2 ml of methylene chloride, and then the resulting solution was subjected to reaction at -10.degree. to -20.degree. C. for 1 hour. Subsequently, into the reaction liquid was dropped a solution of 0.44 g of a benzhydryl ester of 7-amino-3-carbamoyloxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid in 5 ml of methylene chloride at -20.degree. C. After the dropping, the resulting solution was subjected to reaction at -10.degree. to -20.degree. C. for 1.5 hours, and the temperature was then elevated to room temperature. Thereafter, the solvent was removed by distillation under reduced pressure, and the residue was dissolved in 15 ml of ethyl acetate. Subsequently, the resulting solution was successively washed with 10 ml of water, with 5% (weight/weight) aqueous sodium hydrogencarbonate, and saturated aqueous sodium chloride, and then dried over anhydrous magnesium sulfate. The solvent was removed by distillation under reduced pressure. Subsequently, the residue was purified with a column chromatography (silica gel - chloroform), to obtain 0.53 g of benzhydryl ester of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-carbamoyloxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 120.degree.-125.degree. C. (decomp.), yield 71.6%.

IR (KBr) cm.sup.-1 : .nu..sub.NH.sbsb.2 3480, 3380; .nu..sub.CONH 3300; .nu..sub.C=O 1780, 1718, 1680.

NMR (CDCl.sub.3) .tau. values: 0.1 (1H, d), 2.05 (1H, d), 2.64 (15H, bs), 3.19 (1H, s), 4.20 (2H, m), 4.86 (1H, d), 5.20 (2H, s), 5.25 (2H, bs), 6.1 (2H, m), 6.3-6.9 (6H, m), 8.9 (3H, t).

(2) To a solution of 0.2 g of a benzhydryl ester of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-carbamoyloxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid in 5 ml of anisole was added 5 ml of trifluoroacetic acid while ice-cooling, and the resulting mixed solution was reacted for 30 minutes while ice-cooling. After the reaction, the solvent was removed by distillation under reduced pressure. Subsequently, the residue was dissolved in 15 ml of ethyl acetate, and to the resulting solution was added 10 ml of water, and then the resulting solution was adjusted to a pH of 7.5 by addition of sodium hydrogen-carbonate while stirring. Thereafter, the aqueous layer was separated off, added to 20 ml of ethyl acetate, and then adjusted to a pH of 2.0 by addition of 2N hydrochloric acid. Subsequently, the organic layer was separated off, successively washed with water and saturated aqueous sodium chloride, and then dried over anhydrous magnesium sulfate. The solvent was removed by distillation under reduced pressure to deposit crylstals. Subsequently, the deposited crystals were collected by filtration to obtain 0.13 g of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-carbamoyloxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 175.degree.-180.degree. C. (decomp.), yield 83.8%.

IR (KBr) cm.sup.-1 : .nu..sub.NH.sbsb.2 3450, 3350; .nu..sub.CONH.sub.2 3300; .nu..sub.C=O 1778, 1710, 1670. NMR (d.sub.6 -DNSO) .tau. values: 0.15 (1H, d), 0.55 (1H, d), 2.60 (5H, b), 3.47 (2H, s), 4.28 (2H, m), 4.95 (1H, d), 5.25 (2H, q), 6.10 (2H, m), 6.25-6.90 (6H, m), 8.9 (3H, t).

EXAMPLE 49

(1) To a solution of 0.32 g of D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetic acid in 15 ml of anhydrous methylene chloride was added 0.1 g of N-methylmorpholine. The resulting solution was cooled to -20.degree. C., and to the solution was added a solution of 0.11 g of ethyl chlorocarbonate in 1 ml of methylene chloride, and then the resulting mixed solution was reacted at -10.degree. to -20.degree. C. for 1 hour. Subsequently, into the reaction solution was dropped a solution of 0.46 g of .beta.,.beta.,.beta.-trichloroethyl ester of 7-amino-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid in 5 ml of methylene chloride at -20.degree. C. After the dropping, the resulting mixed solution was reacted at -10.degree. to -20.degree. C. for 1 hour, and at room temperature for 30 minutes. After the reaction, the solvent was removed by distillation under reduced pressure, and the residue was successively washed with 5% (weight/weight) aqueous sodium hydrogencarbonate, and with saturated aqueous sodium chloride, and then dried over anhydrous magnesium sulfate. Subsequently, the solvent was removed by distillation under reduced pressure, the residue was purified with a column chromatography (silica gel-benzene-ethyl acetate) to obtain 0.53 g of .beta.,.beta.,.beta.-trichloroethyl ester of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 125.degree.-135.degree. C. (decomp.), yield 69.6%.

IR (KBr) cm.sup.-1 :.nu..sub.C=O 1780, 1715, 1680.

(2) To a solution of 0.5 g of .beta.,.beta.,.beta.-trichloroethyl ester of 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid were added 0.5 g of zinc dust and 0.5 ml of acetic acid, and then the resulting mixture was reacted for 1.5 hours. After the reaction, the reaction liquid was subjected to sellaite filtration, the filtrate was freed from the solvent by distillation under reduced pressure. Thereafter, the residue was dissolved in 15 ml of water, and the resulting solution was adjusted to a pH of 1.5 by addition of 2N hydrochloric acid to deposit crystals. Subsequently, the deposited crystals were collected by filtration, dried and then washed with ethyl acetate to obtain 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, m.p. 163.degree.-165.degree. C. (decomp.), yield 82.1%.

Claims

1. An antibacterial compound of the formula (I), ##STR601## wherein R is a group represented by the formula ##STR602## each of said moieties A, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 is a conventional cephalosporin substituent selected from the radicals or atoms which each of said moieties represents as set forth subsequently, wherein R.sup.5 represents alkyl, cycloalkyl, cycloalkenyl, cycloalkadienyl, aryl, aralkyl, aryloxy, alkylthioalkyl, furyl, thienyl, oxazoly, thiazolyl, isoxazolyl, isothiazolyl, imidazolyl, pyrazolyl, pyridyl, pyrazyl, pyrimidyl, pyridazyl, quinolyl, isoquinolyl, quinazolyl, indolyl, indazolyl, 1,3,4-oxadiazolyl, 1,2,4-oxadiazolyl, 1,3,4-thiadiazolyl or 1,2,4-thiadiazolyl, any of which may be substituted by halogen, hydroxy, nitro, alkyl, alkoxy, alkylthio, acyl or alkyl-sulfonylamino; R.sup.6 represents a hydrogen atom; and R.sup.5 and R.sup.6 together with a common carbon atom may form a cycloalkyl, cycloalkenyl or cycloalkadienyl ring;

R.sup.1 represents a hydrogen atom, a blocking group of a conventional cephalosporin or a pharmaceutically acceptable salt-forming cation of a conventional cephalosporin;

n represents 1 or 2; each of the n X's, which may be identical or different from one another, represents individually an oxygen or sulfur atom, and the n X's are attached in any combination to the 2-, 3-, or 5-positions of the piperazine ring;

m represents 4-n; each pair of R.sup.2 and R.sup.3 are linked to the same carbon atom, and each R.sup.2 and R.sup.3 of m pairs of R.sup.2 and R.sup.3, which may be the same or different, represent individually hydrogen, halogen, carboxyl, alkyl, cycloalkyl, aryl, acyl, aralkyl, alkoxycarbonylalkyl, acyloxyalkyl, alkoxy, alkoxycarbonyl, cycloalkyloxycarbonyl, aralkoxycarbonyl, aryloxycarbonyl, amino, N-alkylamino, N,N-dialkylamino, N-arylamino, cyclic amino or carbamoyl; any of which may be substituted by halogen, alkyl, alkoxy, alkylthio, acyl or nitro; and any pair of R.sup.2 and R.sup.3 together with a common carbon atom may form a cycloalkyl ring.

A represents hydrogen, hydroxy, nitro, cyano, alkyl, alkenyl, alkynyl, alkadienyl, cycloalkyl, cycloalkenyl, cycloalkadienyl, aryl, acyl, aralkyl, acyloxyalkyl, alkoxy, cycloalkyloxy, alkoxycarbonyl, aryloxy, cycloalkyloxycarbonyl, aryloxycarbonyl, aralkoxycarbonyl, alkylsulfonyl, cycloalkylsulfonyl, arylsulfonyl, carbamoyl, N-alkylaminocarbonyl, N-arylaminocarbonyl, N,N-dialkylaminocarbonyl, cyclic aminocarbonyl, thiocarbamoyl, N-alkylaminothiocarbonyl, N-arylaminothiocarbonyl, N,N-dialkylaminothiocarbonyl, cyclic aminothiocarbonyl, acylcarbamoyl, acylthiocarbamoyl, alkylsulfonylcarbamoyl, arylsulfonylcarbamoyl, alkylsulfonylthiocarbamoyl, arylsulfonylthiocarbamoyl, sulfamoyl, alkoxycarbonylthioalkyl, alkoxythiocarbonylthioalkyl, amino, N-alkylamino, N,N-diathylomino, N-acylamino, cyclic amino, thiazolyl, pyridyl, pyridazyl, pyrazyl, thiadiazolyl, triazolyl, tetrazolyl or quinolyl, any of which may be substituted by halogen, hydroxyl, alkyl, alkoxy, alkylthio, nitro, cyano, amino, carboxyl, or acyl;

Y represents an oxygen or sulfur atom; and

R.sup.4 represents hydrogen, halogen, hydroxy, cyano, azido, pyridinium, quinolinium, isoquinolinium, pyrimidinium, alkoxy, aryloxy, aralkoxy, acyloxy, carbamoyloxy, quanidino, amino, N-alkylamino, N,N-dialkylamino, cyclic amino, alkylthio, arylthio, aralkylthio, acylthio, thiocarbamoylthio, alkoxythiocarbonylthio, aryloxythiocarbonylthio, cycloalkyloxythiocarbonylthio, amidinothio, oxazolylthio, thiazolylthio, isoxazolylthio, isothiazolylthio, imidazolylthio, pyrazolylthio, pyridylthio, pyrazinylthio, pyrimidinylthio, pyridazinylthio, quinolylthio, isoquinolylthio, quinazolylthio, indolylthio, indazolylthio, oxadiazolylthio, thiadiazolylthio, [triazolylthio,] tetrazolylthio, triazinylthio, benzimidazolylthio, benzoxazolylthio, benzothiazolylthio, triazolopyridylthio, purinylthio, pyridine-1-oxide-2-ylthio or pyridazine-1-oxide-6-ylthio each of which may be substituted by halogen, alkyl, nitro, cyano, carboxyl or carbamoyl; thiazolinylthio or 3-(2,6-dimethyl-5-oxo-2,5-dihydro-1,2,4-triazinyl)-thio, with the proviso that when R.sup.4 is pyridinium, quinolinium, isoquinolinium, or pyrimidinium there is present a pharmaceutically acceptable counter-ion.

2. An antibacterial compound of claim 1 wherein R.sup.5 represents alkyl, cycloalkadienyl, thienyl, phenyl, p-hydroxy-phenyl; R.sup.6 represents hydrogen; each pair of R.sup.2 and R.sup.3 are linked to the same carbon atom, and each R.sup.2 and R.sup.3 of m pairs of R.sup.2 and R.sup.3, which may be the same or different, represent individually hydrogen, alkyl, aryl or alkoxycarbonylalkyl or any pair of R.sup.2 and R.sup.3 together with a common carbon atom may form a cycloalkyl ring; A represents hydrogen, alkyl, alkenyl, cycloalkyl, aryl, acyl, aralkyl, acyloxyalkyl, alkylsulfonyl, carbamoyl, acylcarbamoyl, N-arylaminocarbonyl or alkoxycarbonyl; and R.sup.4 represents hydrogen, azido, pyridinium, alkoxy, acetoxy, carbamoyloxy, 5-(3-methylisoxazolyl)carbonylthio, alkoxythiocarbonylthio, 4- methyl-1- piperazinothiocarbonylthio; or oxazolylthio, thiazolylthio, imidazolylthio, pyrimidinylthio, oxadiazolylthio, thiadiazolylthio, triazolylthio, pyridine-1-oxide-2-ylthio or tetrazolylthio which may be substituted by alkyl; or 3-(2,6-dimethyl-5-oxo-2,5-dihydro-1,2,4-triazinyl)thio with the proviso that when R.sup.4 is pyridinium there is present a pharmaceutically acceptable counter-ion.

3. An antibacterial compound of claim 1, of the formula, ##STR603##

4. An antibacterial compound of claim 1, of the formula, ##STR604##

5. An antibacterial compound of claim 1, of the formula, ##STR605##

6. An antibacterial compound of claim 1, of the formula, ##STR606##

7. An antibacterial compound of claim 1, of the formula, ##STR607##

8. An antibacterial compound of claim 7, wherein R.sup.5 is phenyl, p-hydroxyphenyl, cycloalkadienyl or thienyl; A is a hydrogen, alkyl, alkenyl, aryl or aralkyl which may be substituted by halogen or hydroxy; each pair of R.sup.2 and R.sup.3 are linked to the same carbon atom, and each R.sup.2 and R.sup.3 of 2 pairs of R.sup.2 and R.sup.3 , which may be the same or different, represent individually hydrogen or alkyl; R.sup.1 represents a hydrogen atom, a pharmaceutically acceptable cation capable of forming a non-toxic salt or an ester-forming group capable of being easily removed by enzymes in a living body; R.sup.4 represents acetoxy, carbamoyloxy, 2-(5-methyl-1,3,4-thiadiazolyl)thio, 2-(1,3,4-thiadiazolyl)-thio, 2-(1-methyl-1,3,4-triazolyl)thio, 5-(1-methyl-1,2,3,4-tetrazolyl)thio 5-(1,2,3,4-tetrazolyl)thio.

9. An antibacterial compound of claim 7, wherein R.sup.5 represents phenyl or p-hydroxyphenyl; each pair of R.sup.2 and R.sup.3 are linked to the same carbon atom and each R.sup.2 and R.sup.3 of 2 pairs of R.sup.2 and R.sup.3, which may be the same or different, represent individually hydrogen or methyl; A represents methyl or ethyl; R.sup.1 represents a hydrogen atom, a pharmaceutically acceptable cation capable of forming a non-toxic salt or an ester-forming group capable of being easily removed by enzymes in a living body; and R.sup.4 represents acetoxy, 2-(5-methyl-1,3,4-thiadizolyl)thio, 2-(1,3,4-thiadiazolyl)thio, 2-(1-methyl-1,3,4-triazolyl)thio, 5-(1-methyl-1,2,3,4-tetrazolyl)thio or 5-(1,2,3,4-tetrazolyl)thio.

10. An antibacterial compound of claim 1, wherein R.sup.1 is selected from the group consisting of ester-forming groups capable of being removed by catalytic reduction, chemical reduction or hydrolysis under mild conditions and ester-forming groups capable of being easily removed by enzymes in a living body.

11. An antibacterial compound of claim 1, wherein R.sup.1 is a pharmaceutically acceptable cation capable of forming a non-toxic salt.

12. An antibacterial compound of claim 1, wherein R.sup.1 is a hydrogen atom.

13. A compound selected from the group consisting of

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonyl-amino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-pentyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-hexyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-heptyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-octyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-iso-propyl-2,3-dioxo-1-piperazinohiocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinothiocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinothiocarbonylamino)phenylacetamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-propyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-butyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-phenyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-6-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4,6-dimethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-phenyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-phenyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)propionamido]-3-acetoxymethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-azidomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1,3,4-triazolyl ) thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-oxadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[3-(2,6-dimethyl-5-oxo-2,5-dihydro-1,2,4-triazinyl) thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(4-methyloxazolyl) thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(4-methylthiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(pyridyl-1-oxide) thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-(2-thiazolinylthiomethyl)-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methylimidazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-(2-pyrimidinylthiomethyl)-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[3-(6-methylpyridazinyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[1-(4-methylpiperazino)-thiocarbonylthiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(3-methylisoxazolyl)-carbonylthiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-ethoxythiocarbonylthiomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-pyridinomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid betaine, and

7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-pyridinomethyl-.DELTA..sup.3 -cephem-4-carboxylic acid betaine.

14. A compound selected from the group consisting of

7-[D(-)-.alpha.-(4-ethoxycarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-n-hexyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-methyl-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methanesulfonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-acetylaminocarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl) thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-acetyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methanesulfonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-ethyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl) thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-acetylaminocarbonyl-2-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[(5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid,

7-[D(-)-.alpha.-(4-methyl-3-oxo-1piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid and

7-[D(-)-.alpha.-(4-ethyl-3-oxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid.

15. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR608## or a non-toxic salt thereof.

16. The compound of claim 7 which is 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR609## or a non-toxic salt thereof.

17. The compound of claim 7 which is 7-[D(-)-.alpha.-(4-methyl-2,3dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR610## or a non-toxic salt thereof.

18. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-ethyl-6-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR611## or a non-toxic salt thereof.

19. The compound of claim 7, which is 7-[D(-)-.alpha.-(4,6-dimethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR612## or a non-toxic salt thereof.

20. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR613## or a non-toxic salt thereof.

21. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR614## or a non-toxic salt thereof.

22. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR615## or a non-toxic salt thereof.

23. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1-methyl-1,3,4-triazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR616## or a non-toxic salt thereof.

24. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl)-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR617## or a non-toxic salt thereof.

25. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula. ##STR618## on a non-toxic salt thereof.

26. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[2-(1,3,4-triazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR619## or a non-toxic salt thereof.

27. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-methyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR620## or a non-toxic salt thereof.

28. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR621## or a non-toxic salt thereof.

29. The compound of claim 7, which is 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR622## or a non-toxic salt thereof.

30. The compound of claim 7, which is 7-[D(-)-.alpha.-(3,5-dioxo-1-piperazinocarbonylamino)-phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(4-acetyl-2,5-dioxo-1-piperazinocarbonyl-amino)phenylacetamido]-3-[2-(5-methyl-1,3,4-thiadiazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, 7-[D(-)-.alpha.-(3,5-dioxo-1-piperazinocarbonylamino)-phenylacetamido]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)-thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid, and 7-[D(-)-.alpha.-(4-acetyl-2,5-dioxo-1-piperazinocarbonylamino)phenylacetamido]-3-[5-(5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid.

31. A pharmaceutical composition comprising an antibacterially effective amount of a compound of claim 1 and a pharmaceutically acceptable carrier.

32. A method of combatting bacteria in mammals which comprises administering to said mammal an antibacterially effective amount of a compound of claim 1.

33. A compound of the formula (I), ##STR623## wherein R is a group represented by the formula ##STR624## each of said moieties A, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 is a conventional cephalosporin substituent selected from the radicals or atoms which each of said moieties represents as set forth subsequently,

wherein

R.sup.5 represents methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclopentyl, cyclohexenyl, cyclopentadienyl, cyclohexadienyl, phenyl, naphthyl, benzyl, phenethyl, phenoxy, naphthoxy, methylthiomethyl, ethylthiomethyl, methylthioethyl, ethylthioethyl, furyl, thienyl, oxazolyl, thiazolyl, isoxazolyl, isothiazolyl, imidazolyl, pyrazolyl, pyridyl, pyrazyl, pyrimidyl, pyridazyl, quinolyl, isoquinolyl, quinazolyl, indolyl, indazolyl, 1,3,4-oxadiazolyl, 1,2,4-oxadiazolyl, 1,3,4-thiadiazolyl, or 1,2,4-thiadiazolyl; each group represented by said R.sup.5 may be substituted by halogen, hydroxy or nitro; R.sup.6 represents a hydrogen atom; and R.sup.5 and R.sup.6 together with a common carbon atom may form a cyclohexyl, cycloheptyl or cyclopentenyl, cyclohexenyl, cyclopentadienyl or cyclohexadienyl ring,

R.sup.1 represents a hydrogen atom, a blocking group of a conventional cephalosporin or a pharmaceutically acceptable salt-forming cation of a conventional cephalosporin;

n represents 1 or 2; each of the n X's, which may be identical or different from one another, represents individually an oxygen or sulfur atom, and the n X's are attached in any combination to the 2-, 3-, or 5-position of the piperazine ring;

m represents 4-n; each pair of R.sup.2 and R.sup.3 are linked to the same carbon atom, and each R.sup.2 and R.sup.3 of m pairs of R.sup.2 and R.sup.3, which may be the same or different, represent individually, a hydrogen atom, a halogen atom, a carboxyl group, methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, cyclopentyl, cyclohexyl, cycloheptyl, phenyl, naphthyl, acetyl, propionyl, butyryl, benzoyl, benzyl, phenethyl, methoxycarbonylmethyl, ethoxycarbonylmethyl, acetyloxymethyl, propionyloxymethyl, pivaloyloxymethyl, benzoyloxymethyl, methoxy, ethoxy, propoxy, butoxy, methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, cyclopentyloxycarbonyl, cyclohexyloxycarbonyl, cycloheptyloxycarbonyl, benzyloxycarbonyl, phenethoxycarbonyl, phenoxycarbonyl, naphthoxycarbonyl, amino, N-methylamino, N-ethylamino, N-propylamino, N-butylamino, N,N-dimethylamino, N,N-diethylamino, N,N-dibutylamino, N-acetylamino, N-propionylamino, N-butyrylamino, N-benzoylamino, pyrrolidino, piperidino, morpholino, carbamoyl, N-methylaminocarbonyl, N-ethylaminocarbonyl, N,N-dimethylaminocarbonyl, N,N-diethylaminocarbonyl, and any pair of R.sup.2 and R.sup.3 together with a common carbon atom may form a cyclopentyl, cyclohexyl or cycloheptyl group, and each of the groups mentioned above for said R.sup.2 and R.sup.3 may be substituted by halogen or nitro;

A represents hydrogen, hydroxyl, nitro, cyano, methyl, ethyl, propyl, isopropyl, butyl, pentyl, hexyl, heptyl, octyl, dodecyl, vinyl, propenyl, butenyl, propargyl, 1,3-butadienyl, 1,3-pentadienyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclopentenyl, cyclohexenyl, cyclopentadienyl, cyclohexadienyl, phenyl, naphthyl, formyl, acetyl, propionyl, isovaleryl, caproyl, enanthoyl, capryloyl, palmitoyl, stearoyl, acryloyl, cyclohexanecarbonyl, benzoyl, phenylglycyl, furoyl, thenoyl, benzyl, phenethyl, acetyloxyethyl, pivaloyloxymethyl, benzoyloxymethyl, methoxy, ethoxy, propoxy, butoxy, cyclopentyloxy, cyclohexyloxy, cycloheptyloxy, phenoxy, naphthoxy, methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, butoxycarbonyl, cyclopentyloxycarbonyl, cyclohexyloxycarbonyl, cycloheptyloxycarbonyl, phenoxycarbonyl, (1- or 2-) naphthoxycarbonyl, benzyloxycarbonyl, phenethoxycarbonyl, methanesulfonyl, ethanesulfonyl, propanesulfonyl, butanesulfonyl, cyclopentanesulfonyl, cyclohexanesulfonyl, benzenesulfonyl, (1- or 2-)naphthalenesulfonyl, carbamoyl, N-methylaminocarbonyl, N-ethylaminocarbonyl, N-propylaminocarbonyl, N-butylaminocarbonyl, N-phenylaminocarbonyl, N,N-dimethylaminocarbonyl, N,N-diethylaminocarbonyl, pyrrolidinocarbonyl, piperidinocarbonyl, morpholinocarbonyl, thiocarbomoyl, N-methylaminothiocarbonyl, N-ethylaminothiocarbonyl, N-propylaminothiocarbonyl, N-phenylaminothiocarbonyl, N,N-dimethylaminothiocarbonyl, N,N-diethylaminothiocarbonyl, pyrrolidinothiocarbonyl, piperidinothiocarbonyl, morpholinothiocarbonyl, N-acetylcarbamoyl, N-propionylcarbomoyl, N-butyrylcarbamoyl, N-benzoylcarbamoyl, N-furoylcarbamoyl, N-thenoylcarbamoyl, N-acetylthiocarbamoyl, N-propionylthiocarbamoyl, N-butyrylthiocarbamoyl, N-benzoylthiocarbamoyl, N-naphthoylthiocarbamoyl, N-furoylthiocarbamoyl, N-thenoylthiocarbamoyl, methanesulfonylaminocarbonyl, ethanesulfonylaminocarbonyl, butanesulfonylaminocarbonyl, benzenesulfonylaminocarbonyl, (1- or 2-)naphthalenesulfonylaminocarbonyl, methanesulfonylaminothiocarbonyl, ethanesulfonylaminothiocarbonyl, butanesulfonylaminothiocarbonyl, benzenesulfonylaminothiocarbonyl, naphthalenesulfonylaminothiocarbonyl, sulfamoyl, N-methylsulfamoyl, N-ethylsulfamoyl, N-propylsulfamoyl, N-butylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N,N-dipropylsulfamoyl, N,N-dibutylsulfamoyl, N-phenylsulfamoyl, N-benzylsulfamoyl, N-cyclopentylsulfamoyl, N-cyclohexylsulfamoyl, methoxycarbonylthiomethyl, ethoxycarbonylthiomethyl, propoxycarbonylthiomethyl, butoxycarbonylthiomethyl, methoxycarbonylthioethyl, methoxythiocarbonylthiomethyl, ethoxythiocarbonylthiomethyl, propoxythiocarbonylthiomethyl, butoxythiocarbonlythiomethyl, methoxythiocarbonylthioethyl, amino, N-methylamino, N-ethylamino, N-propylamino, N-butylamino, N,N-dimethylamino, N,N-diethylamino, N,N-dibutylamino, N-acetylamino, N-propionylamino, N-butyrylamino, N-benzoylamino, pyrrolidino, piperidino, morpholino, thiazolyl, pyridyl, pyridazyl, pyrazyl, thiadiazolyl, triazolyl, tetrazolyl, quinolyl, any of which may be substituted by halogen, hydroxyl, nitro cyano or carboxyl groups;

Y represents on oxygen or sulfur atom;

R.sup.4 represents cyano, azido, pyridinium, quinolinium, isoquinolinium or pyrimidinium, methoxy, ethoxy, propoxy, phenoxy, naphthoxy, phenethoxy, acetyloxy, propionyloxy, butyryloxy, benzoyloxy, naphthoyloxy, cyclopentanecarbonyloxy, cyclohexanecarbonyloxy, furoyloxy, thenoyloxy, carbomoyloxy, N-methylaminocarbonyloxy, N,N-dimethylaminocarbonyloxy, N-acetylaminocarbonyloxy, phenylaminocarbonyloxy, benzylaminocarbonyloxy, cyclohexylaminocarbonyloxy, guanidino, N-methylguanidino, amino, N-methylamino, N-ethylamino, N-propylamino, N-butylamino, N-cyclohexylamino, N-phenylamino, N,N-dimethylamino, N,N-diethylamino, N,N-dibutylamino, pyrrolidino, piperidino, morpholino, methylthio, ethylthio, propylthio, phenylthio, (1- or 2-)-naphthylthio, benzylthio, phenethylthio, acetylthio, propionylthio, butyrylthio, benzoylthio, (1- or 2-)naphthoylthio, cyclopentanecarbonylthio, cyclohexanecarbonylthio, furoylthio, thenoylthio, isothiazolecarbonylthio, isoxazolecarbonylthio, thiadiazolecarbonylthio, thriazolecarbonylthio, thiocarbamoylthio, N-methylthiocarbamoylthio N,N-diethylthiocarbamoylthio, 1-piperidino-thiocarbonylthio, 1-morpholinothiocarbonylthio, 4-methyl-1-piperazinothiocarbonylthio, methoxythiocarbonylthio, ethoxythiocarbonylthio, propoxythiocarbonylthio, butoxythiocarbonylthio, phenoxythiocarbonylthio, cyclohexyloxythiocarbonylthio, amidinothio, N-methylamidinothio, N,N'-dimethylamidinothio, oxazolylthio, thiazolylthio, isoxazolylthio, isothiazolylthio, imidazolylthio, pyrazolylthio, pyridylthio, pyrazinylthio, pyrimidinylthio, pyridazinylthio, quinolylthio, isoquinolylthio, quinazolylthio, indolylthio, indazolylthio, oxadiazolylthio, thiadiazolylthio, triazolylthio, tetrazolylthio, triazinylthio, benzimidazolylthio, benzoxazolylthio, benzothiazolylthio, triazolopyridylthio, purinylthio, pyridine-1-oxide-2-ylthio or pyridazine-1-oxide-6-ylthio, each of which may be substituted by halogen, C.sub.1-4 alkyl, nitro cyano, carboxyl or carbamoyl, thiazolinylthio, or 3-(2,6dimethyl-5-oxo-2,5-dihyro 1,2,4-triazinyl)thio, with the proviso that when R.sup.4 is pyridinium, quinolinium, isoquinolinium, or pyrimidinium there is present a pharmaceutically acceptable counter-ion.

34. A compound of the formula (I), ##STR625## wherein R is a group represented by the formula ##STR626## each of said moieties A, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 is a conventional cephalosporin substitutent selected from the radicals or atoms which each of said moieties represents as set forth subsequently,

wherein

R.sup.5 represents methyl, ethyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclopentenyl, cyclohexenyl, cyclopentadienyl, cyclohexadienyl, phenyl, naphthyl, benzyl, phenoxy, naphthoxy, furyl, thienyl, oxazolyl, thiazolyl, isoxazolyl, isothiazolyl, imidazolyl, pyrazolyl, pyridyl, pyrazyl, pyrimidyl, pyridazyl, quinolyl, isoquinolyl, quinazolyl, indolyl, indazolyl, 1,3,4-oxadiazolyl, 1,2,4-oxadiazolyl, 1,3,4-thiadiazolyl or 1,2,4-thiadiazolyl; each group represented by said R.sup.5 may be substituted by halogen, hydroxy or nitro; R.sup.6 represents a hydrogen atom; and R.sup.5 and R.sup.6 together with a common carbon atom may form a cyclohexyl, cycloheptyl or cyclopentyl, cyclohexenyl, cyclopentadienyl or cyclohexadienyl ring;

R.sup.1 represents a hydrogen atom, a blocking group of a conventional cephalosporin or a pharmaceutically acceptable salt-forming cation of a conventional cephalosporin;

n represents 1 or 2; each of the X's, which may be identical or different from one another, represents individually an oxygen or sulfur atom, and the nX's are attached in any combination to the 2-, 3- or 5-position of the piperazine ring;

m represents 4-n; each pair of R.sup.2 and R.sup.3 are linked to the same carboon atom, and each R.sup.2 and R.sup.3 of m pairs of R.sup.2 and R.sup.3, which may be the same or different, represent individually a hydrogen atom, a halogen atom, a carboxyl group, methyl, ethyl, propyl, cyclopentyl, cyclohexyl, phenyl, naphthyl, acetyl, benzoyl, benzyl, acetyloxymethyl, pivaloyloxymethyl, methoxy, ethoxy, propoxy, methoxycarbonyl, ethoxycarbonyl, cyclohexyloxycarbonyl, benzyloxycarbonyl, phenoxycarbonyl, amino, N-methylamino, N-ethylamino, N,N-dimethylamino, N-acetylamino, carbamoyl, N-methylaminocarbonyl, N,N-dimethylaminocarbonyl, and any pair of R.sup.2 and R.sup.3 together with a common carbon atom may form a cyclopentyl or cyclohexyl group, and each of the groups mentioned above for said R.sup.2 and R.sup.3 may be substituted by halogen;

A represents hydrogen, hydroxyl, nitro, cyano, methyl, ethyl, propyl, vinyl, propenyl, propargyl, 1,3-pentadienyl, cyclopentyl, cyclohexyl, cyclopentenyl, cyclohexenyl, cyclopentadienyl, cyclohexadienyl, phenyl, naphthyl, formyl, acetyl, propionyl, benzoyl, furoyl, thenoyl, benzyl, pivaloyloxymethyl, methoxy, ethoxy cyclopentyloxy, cyclohexyloxy, phenoxy, naphthoxy, methoxycarbonyl, ethoxycarbonyl, cyclopentyloxycarbonyl, cyclohexyloxycarbonyl, phenoxycarbonyl, benzyloxycarbonyl, methanesulfonyl, ethanesulfonyl, cyclohexanesulfonyl, benzenesulfonyl, carbamoly, N-methylaminocarbonyl, N-phenylaminocarbonyl, N,N-dimethylaminocarbonyl, pyrrolidinocarbonyl, piperidinocarbonyl, thiocarbamoyl, N-methylaminothiocarbonyl, N-phenylaminothiocarbonyl, N,N-dimethylaminothiocarbonyl, pyrrolidinothiocarbonyl, piperidinothiocarbonyl, N-acetylcarbamoyl, N-acetylthiocarbamoyl, methanesulfonylaminocarbonyl, methanesulfonylaminothiocarbonyl, sulfamoyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N-phenylsulfamoyl, N-cyclohexylsulfamoyl, amino, N-methylamino, N-ethylamino, N,N-dimethylamino, N-acetylamino, thiazolyl, pyridyl, pyridazyl, pyrazyl, any of which may be substituted by halogen, nitro, cyano or carboxyl groups;

Y represents an oxygen or sulfur atom;

R.sup.4 represents hydrogen, halogen, hydroxy, cyano, azido, pyridinium, quinolinium, isoquinolinium or pyrimidinium, methoxy, ethoxy, propoxy, phenoxy, naphthoxy, benzyloxy, acetyloxy, propionyloxy, butyryloxy, benzoyloxy, naphthoyloxy, cyclopentanecarbonyloxy, thenoyloxy, carbomoyloxy, N-methylaminocarbonyloxy, N,N-dimethylaminocarbonyloxy, N-acetylaminocarbonyloxy, phenylaminocarbonyloxy, benzylaminocarbonyloxy, cyclohexylaminocarbonyloxy, methylthio, ethylthio, propylthio, phenylthio, (1- or 2-)naphthylthio, benzylthio, acetylthio, propionylthio, butyrylthio, benzoylthio, (1- or 2-)naphthoylthio, cyclopentanecarbonylthio, thenoylthio, thiocarbamoylthio, N-methylthiocarbamoylthio, N,N-diethylthiocarbamoylthio, 1-piperidinothiocarbonylthio, 1-morpholinothiocarbonylthio, 4-methyl-1-piperazinothiocarbonylthio, methoxythiocarbonylthio, ethoxythiocarbonylthio, phenoxythiocarbonylthio, cyclohexyloxythiocarbonylthio, oxazolylthio, thiazolylthio, isoxazolylthio, isothiazolylthio, imidazolylthio, pyrazolylthio, pyridylthio, pyrazinylthio, pyrimidinylthio, pyridazinylthio, quinolythio, isoquinolylthio, quinazolylthio, indolylthio, indazolylthio, oxadiazolylthio, thiadiazolylthio, triazolylthio, tetrazolylthio, triazinylthio, benzimidazolylthio, benzoxazolylthio, benzothiazolylthio, triazolopyridylthio, purinylthio, each of which may be substituted by halogen, C.sub.1-4 alkyl, nitro, cyano, carboxyl or carbamoyls, thiazolinylthio, or 3-(2,6-dimethyl-5-oxo-2,5dihydro-1,2,4-triazinyl)thio, with the proviso that when R.sup.4 is pyridinium, quinolinium, isoquinolinium, or pyrimidinium there is present a pharamceutically acceptable counter-ion.

35. A compound of claim 34, wherein R.sup.5 represents methyl, ethyl, cyclopentadienyl, cyclohexadienyl, phenyl or 2-thienyl, each of which may be substituted by halogen, hydroxy or nitro; R.sup.6 represents a hydrogen atom; each pair of R.sup.2 and R.sup.3 are linked to the same carbon atom, and each R.sup.2 and R.sup.3 of m pairs of R.sup.2 and R.sup.3, which may be the same or different, represent individually a hydrogen atom, methyl, ethyl, propyl, or phenyl; A represents hydrogen, methyl, ethyl, propyl, vinyl, propenyl, benzyl or phenyl, any of which may be optionally substituted by a halogen atom; R.sup.4 represents acetoxy, carbamoyloxy or (1-methyl-1,2,3,4-tetrazolyl)thio groups; and R.sup.1 is a hydrogen atom, a conventional cephalosporin cation capable of forming a pharmaceutically acceptable non-toxic salt or a conventional cephalosporin ester forming group capable of being easily removed by enzymes in a living body.

36. A compound of claim 34, wherein R.sup.5 represents phenyl or 4-hydroxyphenyl; R.sup.6 represents hydrogen; each pair of R.sup.2 and R.sup.3 are linked to the same carbon atom, and each R.sup.2 and R.sup.3 of m pairs of R.sup.2 and R.sup.3, which may be the same or different, are individually a hydrogen atom or methyl; A represents methyl or ethyl; R.sup.4 represents acetoxy or (1-methoxy-1,2,3,4-tetrazolyl)thio groups; and R.sup.1 is a hydrogen atom, or a conventional cephlosporin cation capable of forming a pharamceutically acceptable non-toxic salt.

37. The compound of claim 36, which is 7-D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinocarbonylamino)-p-hydroxyphenylacetamide]-3-[5-(1-methyl-1,2,3,4-tetrazolyl)thiomethyl]-.DELTA..sup.3 -cephem-4-carboxylic acid of the formula, ##STR627## or its sodium salt.

38. A D(-)-isomer of a compound of ##STR628## wherein Y is hydrogen, R.sup.1 is hydrogen or halogen, R.sup.2 is lower alkyl and R.sup.3 is hydrogen or lower alkyl,and pharmaceutically acceptable salts thereof.

39. A compound as claimed in claim 38, wherein the pharmaceutically acceptable salt is an alkali metal salt.

40. A compound as claimed in claim 38, wherein the pharmaceutically acceptable salt is the sodium or potassium salt.

41. A pharmaceutical composition having antibacterial activity which comprises an effective amount of a compound as claimed in claim 38 and a pharmaceutically acceptable carrier therefor.

42. A D(-)-isomer of a compound of the formula (I): ##STR629## wherein Y is hydrogen, R.sup.1 is hydrogen, R.sup.2 is lower alkyl and R.sup.3 is hydrogen or lower alkyl, and pharmaceutically acceptable salts thereof.

43. A compound as claimed in claim 42, wherein the pharmaceutically acceptable salt is an alkali metal salt.

44. A compound as claimed in claim 42, wherein the pharmaceutically acceptable salt is the sodium or potassium salt.

45. 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxopiperazin-1-yl-carbonylamino)-p-hydroxyphenylacetamido]-3-(1,3,4-thiadiazol-2-yl-thiomethyl)-3-cephem-4-carboxylic acid as claimed in claim 42.

46. 7-[D(-)-.alpha.-(4-ethyl-2,3-dioxopiperazin-1-yl-carbonylamino)-p-hydroxyphenylacetamido]-3-(5-methyl-1,3,4-thiadiazol-2-yl-thiomethyl)-3-cephem-4-carboxylic acid as claimed in claim 42.

47. A pharmaceutical composition having antibacterial activity which comprises an effective amount of a compound as claimed in claim 42 and a pharmaceutically acceptable carrier therefor.