Claims
- 1. A dioxetane of the formula ##STR4##
- 2. A dioxetane of the formula ##STR5## wherein R is Cl-3 alkyl and wherein X is selected from the group consisting of phosphate, galactoside, acetate, 1-phospho-2,3-diacylglyceride, 1-thio-D-glucoside, adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, adenosine, .alpha.-D-glucoside, .beta.-D-glucoside, .beta.-D-glucuronide,.alpha.-D-mannoside, .beta.-D-mannoside, .beta.-D-fructofuranoside, .beta.-glucosiduranate, P-toluenesulfonyl-L-arginine ester, and P-toluenesulfonyl-L-arginine amide.
- 3. A method of detecting the presence of alkaline phosphatase in a sample comprising contacting said sample with a dioxetane of claims 1 or 2, and detecting release of light caused thereby, wherein detection of light released indicates presence of alkaline phosphatase in a sample.
- 4. A method of detecting a first member of a specific binding pair having first and second members, comprising:
- optically detecting chemiluminescence produced by the reaction of a dioxetane of claim 1 or 2 with an enzyme which cleaves said enzyme-cleavable group X of said dioxetane, wherein said enzyme is complexed with a second member of said specific binding pair and correlating the chemiluminscene detected with the presence of the first member of said specific binding pair.
- 5. A kit for conducting a chemiluminescent assay comprising:
- a dioxetane of claim 1 or 2, and
- an enzyme which cleaves the enzyme-cleavable group X of said dioxetane.
- 6. The kit of claim 4, further comprising a membrane on which said assay may be conducted.
Parent Case Info
This application is CIP of copending U.S. application Ser. No. 08/057,903, filed May 7, 1993 which is incorporated herein by reference, which is a CIP of U.S. application Ser. No. 07/806,925 filed Dec. 11, 1991, now abandoned, which is in turn a divisional application of U.S. application Ser. No. 07/574,786 filed Aug. 30, 1990 now U.S. Pat. No. 5,112,960, a CIP of U.S. application Ser. No. 07/559,152 filed Jul. 25, 1990, now abandoned, itself a divisional application of U.S. application Ser. No. 07/367,772 filed Jul. 17, 1989, now abandoned.
US Referenced Citations (6)
Foreign Referenced Citations (1)
Number |
Date |
Country |
9204341 |
Mar 1992 |
WOX |
Non-Patent Literature Citations (1)
Entry |
Chemical Abstract, vol. 117, AN 107794, WO 92/04341, Mar. 19, 1992. |
Divisions (2)
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Number |
Date |
Country |
Parent |
574786 |
Aug 1990 |
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Parent |
367772 |
Jul 1989 |
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Continuation in Parts (3)
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Number |
Date |
Country |
Parent |
57903 |
May 1993 |
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Parent |
806925 |
Dec 1991 |
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Parent |
559152 |
Jul 1990 |
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