Claims
- 1. A recombinant adenovirus comprising a chimeric adenoviral fiber protein comprising a non-adenoviral amino acid sequence in place of or in addition to a native fiber amino acid sequence, wherein the chimeric adenoviral fiber protein comprises a trimerization domain and the non-adenoviral amino acid sequence is of a size such that folding of the chimeric adenoviral fiber protein and assembly of a complex comprising the chimeric adenoviral fiber protein and a penton base is not impeded.
- 2. The recombinant adenovirus of claim 1, wherein the non-adenoviral amino acid sequence in the chimeric adenoviral fiber protein is a protein binding sequence
- 3. The recombinant adenovirus of claim 2, wherein the protein binding sequence is selected from the group consisting of a bispecific protein binding sequence and a multispecific protein binding sequence.
- 4. The recombinant adenovirus of claim 2, wherein the non-adenoviral amino acid sequence is located at the C-terminus of the chimeric fiber protein.
- 5. The recombinant adenovirus of claim 2, which further comprises a chimeric coat protein comprising a nonnative amino acid sequence that is specific for binding to a protein.
- 6. The recombinant adenovirus of claim 5, wherein the chimeric coat protein is selected from the group consisting of a chimeric penton base and a chimeric hexon.
- 7. The recombinant adenovirus of claim 1, wherein a native fiber receptor binding sequence in the chimeric adenoviral fiber protein is inactive.
- 8. A viral transfer vector comprising a chimeric adenoviral fiber protein comprising a non-adenoviral amino acid sequence in place of or in addition to a native fiber amino acid sequence, wherein the chimeric adenoviral fiber protein comprises a trimerization domain and the non-adenoviral amino acid sequence is of a size such that folding of the chimeric adenoviral fiber protein and assembly of a complex comprising the chimeric adenoviral fiber protein and a penton base is not impeded.
- 9. The viral transfer vector of claim 8, wherein the viral transfer vector is an adenoviral transfer vector.
- 10. The viral transfer vector of claim 9, wherein the non-adenoviral amino acid sequence in the chimeric fiber protein is a protein binding sequence.
- 11. The viral transfer vector of claim 10, wherein the protein binding sequence is selected from the group consisting of a bispecific protein binding sequence and a multispecific binding sequence.
- 12. The viral transfer vector of claim 10, wherein the non-adenoviral amino acid sequence is located at the C-terminus of the chimeric fiber protein.
- 13. The viral transfer vector of claim 9, wherein a native fiber receptor binding sequence in the chimeric adenoviral fiber protein is inactive.
CROSS-REFERENCE TO RELATED PATENT APPLICATIONS
[0001] This patent application is a continuation of copending U.S. patent application Ser. No. 08/906,758, filed Aug. 5, 1997, which is a continuation of U.S. patent application Ser. No. 08/395,381, filed Feb. 21, 1995, now U.S. Pat. No. 5,770,442.
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT
[0002] This invention was made in part with United States Government support under Grant Number HL51746-01 awarded by the National Institutes of Health. The Government may have certain rights in this invention.
Continuations (2)
|
Number |
Date |
Country |
Parent |
08906758 |
Aug 1997 |
US |
Child |
10173930 |
Jun 2002 |
US |
Parent |
08395381 |
Feb 1995 |
US |
Child |
08906758 |
Aug 1997 |
US |