Claims
- 1. A chimeric adenovirus fiber protein comprising a nonnative amino acid sequence, wherein said protein trimerizes when produced in a mammalian cell.
- 2. The chimeric adenovirus fiber protein of claim 1, wherein said nonnative amino acid sequence comprises a trimerization domain.
- 3. The chimeric adenovirus fiber protein of claim 2, wherein said trimerization domain is derived from an adenovirus fiber from an adenovirus serotype other than that from which said fiber protein is derived.
- 4. The chimeric adenovirus fiber protein of claim 2, wherein said trimerization domain is a non-adenoviral polypeptide.
- 5. The chimeric adenovirus fiber protein of claim 1, wherein said nonnative amino acid sequence is located internally in said chimeric adenovirus fiber protein.
- 6. The chimeric adenovirus fiber protein of claim 1, wherein said nonnative amino acid sequence is located at the C-terminus of said chimeric adenovirus fiber protein.
- 7. The chimeric adenovirus fiber protein of claim 1, wherein said nonnative amino acid sequence is in place of a native fiber amino acid.
- 8. The chimeric adenovirus fiber protein of claim 1, wherein said nonnative amino acid sequence is in addition to a native fiber amino acid.
- 9. The chimeric adenovirus fiber protein of claim 1, wherein said nonnative amino acid sequence is joined to said chimeric adenovirus fiber protein by at least one spacer sequence.
- 10. The chimeric adenovirus fiber protein of claim 1, wherein said nonnative amino acid sequence comprises a protein-specific amino acid sequence.
- 11. The chimeric adenovirus fiber protein of claim 1, which lacks a native amino acid receptor-binding sequence.
- 12. A trimer comprising the chimeric adenovirus fiber protein of claim 1.
- 13. A recombinant adenovirus comprising the chimeric adenovirus fiber protein of claim 1.
- 14. An isolated and substantially purified nucleic acid that encodes the chimeric adenovirus fiber protein of claim 1.
- 15. A chimeric adenovirus fiber protein lacking a native amino acid receptor-binding sequence, wherein said protein trimerizes when produced in a mammalian cell.
- 16. The chimeric adenovirus fiber of claim 15, wherein said native amino acid receptor-binding sequence is displaced by insertional mutagenesis.
- 17. The chimeric adenovirus fiber of claim 15, comprising a nonnative amino acid sequence.
- 18. The chimeric adenovirus fiber of claim 17, wherein said nonnative amino acid sequence comprises a protein-specific amino acid sequence.
- 19. An adenovirus comprising the chimeric adenovirus fiber of claim 15.
- 20. The adenovirus of claim 19, wherein said adenovirus is targeted via an adenovirus penton protein.
- 21. The adenovirus of claim 20, wherein said penton protein comprises a nonnative amino acid sequence.
- 22. The adenovirus of claim 19, wherein said adenovirus is targeted via an adenovirus hexon protein.
- 23. The adenovirus of claim 22, wherein said hexon protein comprises a nonnative amino acid sequence.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This is a continuation application of copending U.S. patent application Ser. No. 08/908,758, which is a continuation application of U.S. patent application Ser. No. 08/395,381, filed Feb. 21, 1995, now U.S. Pat. No. 5,770,442.
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT
[0002] This invention was made in part with United States Government support under Grant Number HL5 1746-01 awarded by the National Institutes of Health. The United States Government may have certain rights in this invention.
Continuations (2)
|
Number |
Date |
Country |
Parent |
08906758 |
Aug 1997 |
US |
Child |
09326447 |
Jun 1999 |
US |
Parent |
08395381 |
Feb 1995 |
US |
Child |
08906758 |
Aug 1997 |
US |