Cloned DNA sequences related to the entire genomic RNA of human immunodeficiency virus II (HIV-2), polypeptides encoded by these DNA sequences and use of these DNA clones and polypeptides in diagnostic kits

Abstract
A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In one embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.
Description


BACKGROUND OF THE INVENTION

[0002] The invention relates to cloned DNA sequences analogous to the genomic RNA of a virus known as Lymphadenopathy-Associated Virus II (“LAV-II”), a process for the preparation of these cloned DNA sequences, and their use as probes in diagnostic kits. In one embodiment, the invention relates to a cloned DNA sequence analogous to the entire genomic RNA of HIV-2 and its use as a probe. The invention also relates to polypeptides with amino acid sequences encoded by these cloned DNA sequences and the use of these polypeptides in diagnostic kits.


[0003] According to recently adopted nomenclature, as reported in Nature, May 1986, a substantially-identical group of retroviruses which has been identified as one causative agent of AIDS are now referred to as Human Immunodeficiency Viruses I (HIV-1). This previously-described group of retroviruses includes Lymphadenopathy-Associated Virus I (LAV-I), Human T-cell Lymphotropic Virus-III (HTLV-III), and AIDS-Related Virus (ARV).


[0004] Lymphadenopathy-Associated Virus II has been described in U.S. application Ser. No. 835,228, which was filed Mar. 3, 1986, and is specifically incorporated herein by reference. Because LAV-II is a second, distinct causative agent of AIDS, LAV-II properly is classifiable as a Human Immunodeficiency Virus II (HIV-2). Therefore, “LAV-II” as used hereinafter describes a particular genus of HIV-2 isolates.


[0005] While HIV-2 is related to HIV-1 by its morphology, its tropism and its in vitro cytopathic effect on CD4 (T4) positive cell lines and lymphocytes, HIV-2 differs from previously described human retroviruses known to be responsible for AIDS. Moreover, the proteins of HIV-1 and 2 have different sizes and their serological cross-reactivity is restricted mostly to the major core protein, as the envelope glycoproteins of HIV-2 are not immune precipitated by HIV-1-positive sera except in some cases where very faint cross-reactivity can be detected. Since a significant proportion of the HIV infected patients lack antibodies to the major core protein of their infecting virus, it is important to include antigens of both HIV-1 and HIV-2 in an effective serum test for the diagnosis of the infection by these viruses.


[0006] HIV-2 was first discovered in the course of serological research on patients native to Guinea-Bissau who exhibited clinical and immunological symptoms of AIDS and from whom sero-negative or weakly sero-positive reactions to tests using an HIV-1 lysate were obtained. Further clinical studies on these patients isolated viruses which were subsequently named “LAV-II.”


[0007] One LAV-II isolate was deposited at the Collection Nationale des Cultures de Micro-Organismes (C.N.C.M.) at the Institut Pasteur in Paris, France on Dec. 19, 1985 under Accession No. I-502. A second LAV-II isolate was deposited at C.N.C.M. on Feb. 21, 1986 under Accession No. I-532. This second isolate has been subsequently referred to a LAV-II ROD. Several additional isolates have been obtained from West African patients, some of whom have AIDS, others with AIDS-related conditions and others with no AIDS symptoms. All of these viruses have been isolated on normal human lymphocyte cultures and some of them were thereafter propagated on lymphoid tumor cell lines such as CEM and MOLT.


[0008] Due to the sero-negative or weak sero-positive results obtained when using kits designed to identify HIV-1 infections in the diagnosis of these new patients with HIV-2 disease, it has been necessary to devise a new diagnostic kit capable of detecting HIV-2 infection, either by itself or in combination with an HIV-1 infection. The present inventors have, through the development of cloned DNA sequences analogous to at least a portion of the genomic RNA of LAV-II ROD viruses, created the materials necessary for the development of such kits.



SUMMARY OF THE INVENTION

[0009] As noted previously, the present invention relates to the cloned nucleotide sequences homologous or identical to at least a portion of the genomic RNA of HIV-2 viruses and to polypeptides encoded by the same. The present invention also relates to kits capable of diagnosing an HIV-2 infection.


[0010] Thus, a main object of the present invention is to provide a kit capable of diagnosing an infection caused by the HIV-2 virus. This kit may operate by detecting at least a portion of the RNA genome of the HIV-2 virus or the provirus present in the infected cells through hybridization with a DNA probe or it may operate through the immunodiagnostic detection of polypeptides unique to the HIV-2 virus.


[0011] Additional objects and advantages of the present invention will be set forth in part in the description which follows, or may be learned from practice of the invention. The objects and advantages may be realized and attained by means of the instrumentalities and combinations particularly pointed out in the appended claims.


[0012] To achieve these objects and in accordance with the purposes of the present invention, cloned DNA sequences related to the entire genomic RNA of the LAV-II virus are set forth. These sequences are analogous specifically to the entire genome of the LAV-II ROD strain.


[0013] To further achieve the objects and in accordance with the purposes of the present invention, a kit capable of diagnosing an HIV-2 infection is described. This kit, in one embodiment, contains the cloned DNA sequences of this invention which are capable of hybridizing to viral RNA or analogous DNA sequences to indicate the presence of an HIV-2 infection. Different diagnostic techniques can be used which include, but are not limited to: (1) Southern blot procedures to identify cellular DNA which may or may not be digested with restriction enzymes; (2) Northern blot techniques to identify RNA extracted from cells, body fluids or culture supernatants; and (3) dot blot techniques, i.e., direct filtration of the sample through an ad hoc membrane such as nitrocellulose or nylon without previous separation on agarose gel. Suitable material for dot blot technique could be obtained from body fluids including, but not limited to, serum and plasma, supernatants from culture cells, or cytoplasmic extracts obtained after cell lysis and removal of membranes and nuclei of the cells by centrifugation as accomplished in the “CYTODOT” procedure as described in a booklet published by Schleicher and Schull.


[0014] In an alternate embodiment, the kit contains the polypeptides created using these cloned DNA sequences. These polypeptides are capable of reacting with antibodies to the HIV-2 virus present in sera of infected individuals, thus yielding an immunodiagnostic complex.


[0015] It is understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention as claimed. The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate one embodiment of the invention and, together with the description, serve to explain the principles of the invention.







BRIEF DESCRIPTION OF THE DRAWINGS

[0016]
FIG. 1 generally depicts the nucleotide sequence of a cloned complementary DNA (cDNA) to the genomic RNA of HIV-2. FIG. 1A depicts the genetic organization of HIV-1, position of the HIV-1 HindIII fragment used as a probe to screen the cDNA library, and restriction map of the HIV-2 cDNA clone, E2. FIG. 1B depicts the nucleotide sequence of the 3′ end of the 3′ LTR of HIV-2. The corresponding region of the HIV-1 LTR was aligned using the Wilburg and Lipman algorithm (window: 10; K-tuple: 7; gap penalty: 3 as described by U. Gublu and B. J. Hoffman in Gene 25: 263-269 (1983), specifically incorporated herein by reference). The U3-R junction in HIV-1 is indicated and the poly A addition signal and potential TATA promoter regions are boxed. In FIG. 1A, the symbols B, H, Ps and Pv refer to the restriction sites BamHI, HindIII, PstI and PvuII, respectively.


[0017]
FIG. 2 generally depicts the HIV-2 specificity of the E2 clone. FIG. 2A and B specifically depict a Southern blot of DNA extracted from CEM cells infected with the following isolates: λHIV-2ROD (a,c), HIV-2DUL (b,d), and HIV-1BRU e, f. Blots a, b, f are PstI digested. Blots c, d, e are undigested. FIG. 2C and D specifically depict dot blot hybridization of pelleted virions from CEM cells infected by the HIV-1BRU(1), SIV isolate Mm 142-83 (3), HIV-2DUL (4), λHIV-2ROD (5), and HIV1ELI (6). Dot 2 is a pellet from an equivalent volume of supernatant from uninfected CEM. Thus, FIGS. 2A and C depict hybridization with the HIV-2 cDNA (E2) and FIGS. 2B and D depict hybridization to an HIV-1 probe consisting of a 9.2 Kb SacI insert from HIV-1BRU.


[0018]
FIG. 3 generally depicts a restriction map of the HIV-2 ROD genome and its homology to HIV-1. FIG. 3A specifically depicts the organization of three recombinant phage lambda clones, ROD 4, ROD 27, and ROD 35. In FIG. 3A, the open boxes represent viral sequences, the LTR are filled, and the dotted boxes represent cellular flanking sequences (not mapped). Only some characteristic restriction enzyme sites are indicated. ROD 27 and 35 are derived from integrated proviruses while ROD 4 is derived from a circular viral DNA. The portion of the lambda clones that hybridizes to the CDNA E2 is indicated below the maps. A restriction map of the λROD isolate was reconstructed from these three lambda clones. In this map, the restriction sites are identified as follows: B: BamHI; E: EcoRI; H: HindIII; K: KpnI; Ps: PstI; Pv: PvuII; S: SacI; X: XbaI. R and L are the right and left BamHI arms of the lambda L47.1 vector.


[0019]
FIG. 3B specifically depicts dots 1-11 which correspond to the single-stranded DNA form of M13 subclones from the HIV-1BRU cloned genome. Their size and position on the HIV-1 genome, determined by sequencing is shown below the figure. Dot 12 is a control containing lambda phage DNA. The dot-blot was hybridized in low stringency conditions as described in Example 3 with the complete lambda ROD 4 clone as a probe, and successively washed in 2×SSC, 0.1% SDS at 25° C. (Tm −42° C.), 1×SSC, 0.1% SDS at 60° C. (Tm −20° C.), and 0.1×SSC, 0.1% SDS at 60° C. (Tm −3° C.) and exposed overnight. A duplicate dot blot was hybridized and washed in stringent conditions (as described in Example 4) with the labelled lambda J19 clone carrying the complete HIV-1BRU genome. HIV-1 and HIV-2 probes were labelled the same specific activity (108 cpm/μg.).


[0020]
FIG. 4 generally depicts the restriction map polymorphism in different HIV-2 isolates and shows comparison of HIV-2 to SIV. FIG. 4A specifically depicts DNA (20 μg per lane) from CEM cells infected by the isolate HIV-2DUL (panel 1) or peripheral blood lymphocytes (PBL) infected by the isolates HIV-2GOM (panel 2) and HIV-2MIR (panel 3) digested with: EcoRI (a), PstI (b), and HindIII (c). Much less viral DNA was obtained with HIV-2 isolates propagated on PBL. Hybridization and washing were in stringent conditions, as described in Example 4, with 106 cpm/ml of each of the E2 insert (cDNA) and the 5 kb. HindIII fragment of λROD 4, labelled to 109 cpm/μg.


[0021]
FIG. 4B specifically depicts DNA from HUT 78 (a human T lymphoid cell line) cells infected with SIV Mm 142-83. The same amounts of DNA and enzymes were used as used in panel A. Hybridization was performed with the same probe as in A, but in non-stringent conditions. As described in Example 3 washing was for one hour in 2×SSC, 0.1% SDS at 40° C. (panel 1) and after exposure, the same filter was re-washed in 0.1×SSC, 0.1% SDS at 60° C. (panel 2). The autoradiographs were obtained after overnight exposure with intensifying screens.


[0022]
FIG. 5 is a restriction map of the LAV-II ROD genome.







DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0023] Reference will now be made in detail to the presently preferred embodiments of the invention, which, together with the following examples, serve to explain the principles of the invention.


[0024] The genetic sequence of a portion of the HIV-2 virus has been determined according to the method set forth in the examples. A restriction map of the genome of this virus is set forth in FIG. 5. In addition, a portion of this sequence, in particular the poly A tail, the end of the U3 region and the total R region have been sequenced. This sequence information is included in Example 2, infra.


[0025] From this sequence data, certain regions have been identified which are capable of being used as probes in diagnostic methods to obtain the immunological reagents necessary to diagnose an HIV-2 infection. In particular, these probes may be used in hybridization reactions with the genetic material of infected patients to indicate whether the RNA of the HIV-2 virus is present in these patients' lymphocytes. In this embodiment, the test methods which may be utilized include Northern blots, Southern blots and dot blots.


[0026] In addition, the genetic sequences of the HIV-2 virus may be used to create the polypeptides encoded by these sequences. Specifically, these polypeptides may be created by expression of the CDNA obtained in the following Examples in hosts such as bacteria, yeast or animal cells. These polypeptides may be used in diagnostic tests such as immunofluorescense assays (IFA), radioimmunoassays (RIA) and Western blot tests. One particular polypeptide which is useful in these diagnostic procedures is the expression product of the cDNA clone analogous to the total R region of the HIV-2 genome.


[0027] In addition to the cDNA sequences described in the following examples, other DNA and/or RNA sequences can be isolated which will be identical or homologous to the genome of various HIV-2 isolates. These new sequences also may serve as the basis for additional immunodiagnostic and probe tests of the type described above. These other DNA and/or RNA sequences may be isolated according to methods routinely known to those of ordinary skill in the art once they have been apprised of the teachings contained herein.


[0028] Moreover, it is also contemplated that additional diagnostic tests, including additional immunodiagnostic tests, may be developed in which the DNA probes or the polypeptides of this invention may serve as one of the diagnostic reagents. The invention described herein includes these additional test methods.


[0029] The genetic structure of the HIV-2 virus has been analyzed by molecular cloning according to the method set forth herein and in the Examples. A restriction map of the genome of this virus is included in FIG. 4. In addition, the partial sequence of a CDNA complementary to the genomic RNA of the virus has been determined. This CDNA sequence information is included in FIG. 1.


[0030] Also contained herein is data describing the molecular cloning of the complete 9.5 kb. genome of HIV-2, data describing the observation of restriction map polymorphism between different isolates, and an analysis of the relationship between HIV-2 and other human and simian retroviruses. From the totality of these data, diagnostic probes can be discerned and prepared.


[0031] Generally, to practice one embodiment of the present invention, a series of filter hybridizations of the HIV-2 RNA genome with probes derived from the complete cloned HIV-1 genome and from the qaq and pol genes were conducted. These hybridizations yielded only extremely weak signals even in conditions of very low stringency of hybridization and washing. Thus, it was found to be difficult to assess the amount of HIV-2 viral and proviral DNA in infected cells by Southern blot techniques.


[0032] Therefore, a complementary DNA (cDNA) to the HIV-2 genomic RNA initially was cloned in order to provide a specific hybridization probe. To construct this cDNA, an oligo (dT) primed cDNA first-strand was made in a detergent-activated endogenous reaction using HIV-2 reverse transcriptase with virions purified from supernatants of infected CEM cells. The CEM cell line is a lymphoblastoid CD4+ cell line described by G.E. Foley et al. in Cancer 18: 522-529 (1965), specifically incorporated herein by reference. The CEM cells used were infected with the isolate ROD and were continuously producing high amounts of HIV-2.


[0033] After second-strand synthesis, the cDNAs were inserted into the TG130 m13 bacteria phage vector. A collection of 104 M13 recombinant phages was obtained and screened in situ with an HIV-1 probe spanning 1.5 kb of the 3′ end of the LAVBRU isolate (depicted in FIG. 1A). Some 50 positive plaques were detected, purified, and characterized by end sequencing and crosshybridizing the inserts. This procedure is described in more detail in Example 3 and in FIG. 1.


[0034] The different clones were found to be complementary to the 3′ end of a polyadenylated RNA having the AATAAA signal about 20 nucleotides upstream of the poly A tail, as found in the long terminal repeat (LTR) of HIV-1. The LTR region of HIV-1 has been described by S. Wain Hobson et al. in Cell 40: 9-17 (1985), specifically incorporated herein by reference. The portion of the HIV-2 LTR that was sequenced was related only distantly to the homologous domain in HIV-1 as demonstrated in FIG. 1B. Indeed, only about 50% of the nucleotides could be aligned and about a hundred insertions/deletions need to be introduced. In comparison, the homology of the corresponding domains in HIV-1 isolates from USA and Africa is greater than 95% and no insertions or deletions are seen.


[0035] The largest insert of this group of M13 clones was a 2 kb clone designated E2. Clone E2 was used as a probe to demonstrate its HIV-2 specificity in a series of filter hybridization experiments. Firstly, this probe could detect the genomic RNA of HIV-2 but not HIV-1 in stringent conditions as shown in FIG. 2, C and D. Secondly, positive signals were detected in Southern blots of DNA from cells infected with the ROD isolate as well as other isolates of HIV-2 as shown in FIG. 2, A and FIG. 4, A. No signal was detected with DNA from uninfected cells or HIV-1 infected cells, confirming the exogenous nature of HIV-2. In undigested DNA from HIV-2 infected cells, an approximately 10 kb species, probably corresponding to linear unintegrated viral DNA, was principally detected along with a species with an apparent size of 6 kb, likely to be the circular form of the viral DNA. Conversely, rehybridization of the same filter with an HIV-1 probe under stringent conditions showed hybridization to HIV-1 infected cells only as depicted in FIG. 2B.


[0036] To isolate the remainder of the genome of HIV-2, a genomic library in lambda phage L47.1 was constructed. Lambda phage L47.1 has been described by W. A. M. Loenen et al. in Gene 10: 249-259 (1980), specifically incorporated herein by reference. The genomic library was constructed with a partial Sau3AI restriction digest of the DNA from the CEM cell line infected with HIV-2ROD.


[0037] About 2×106 recombinant plaques were screened in situ with labelled insert from the E2 cDNA clone. Ten recombinant phages were detected and plaque purified. Of these phages, three were characterized by restriction mapping and Southern blot hybridization with the E2 insert and probes from its 3′ end (LTR) or 5′ end (envelope), as well as with HIV-1 subgenomic probes. In this instance, HIV-1 probes were used under non-stringent conditions.


[0038] A clone carrying a 9.5 kb. insert and derived from a circular viral DNA was identified as containing the complete genome and designated λROD 4. Two other clones, λROD 27 and λROD 35 were derived from integrated proviruses and found to carry an LTR and cellular flanking sequences and a portion of the viral coding sequences as shown in FIG. 3, A. The λROD 4 fragment containing the genome of HIV-2 may be found in the genome of the plasmid pROD 4.7 in E. coli TG1 stored under C.N.C.M. I-627; the λROD 27 fragment may be found in the genome of the plasmid pROD 27.5 in E. coli HB101 stored under C.N.C.M. I626; and another ROD fragment containing the genome of HIV-2 may be found on plasmid pROD 4.8 in E. coli TG1 stored under C.N.C.M. I628 (all deposits made Nov. 21, 1986).


[0039] The relationship of HIV-2 to other human and simian retroviruses was surmised from hybridization experiments. The relative homology of the different regions of the HIV-1 and 2 genomes was determined by hybridization of fragments of the cloned HIV-1 genome with the labelled λROD 4 expected to contain the complete HIV-2 genome (FIG. 3B). Even in very low stringency conditions (Tm −42° C.), the hybridization of HIV-1 and 2 was restricted to a fraction of their genomes, principally the qaq gene (dots 1 and 2), the reverse transcriptase domain in pol (dot 3), the end of pol and the Q (or sor) genes (dot 5) and the F gene (or 3′ orf) and 3′ LTR (dot 11). The HIV-1 fragment used to detect the HIV-2 cDNA clones contained the dot 11 subclone, which hybridized well to HIV-2 under non-stringent conditions. Only the signal from dot 5 persisted after stringent washing. The envelope gene, the region of the tat gene and a part of pol thus seemed very divergent. These data, along with the LTR sequence obtained (FIG. 1B), indicated that HIV-2 is not an envelope variant of HIV-1 as described by M. Alizon et al. in Cell 46: 63-74 (1986).


[0040] It was observed that HIV-2 is related more closely to the Simian Immunodeficiency Virus (SIV) than it is to HIV-1 . This correlation has been described by F. Clavel et al. in C.R. Acad. Sci. (Paris) 302: 485-488 (1986) and F. Clavel et al. in Science 233: 343-346 (1986), both of which are specifically incorporated herein by reference. Simian Immunodeficiency Virus (also designated Simian T-cell Lymphotropic Virus Type 3, STLV-3) is a retrovirus first isolated from captive macaques with an AIDS-like disease in the USA. This simian virus has been described by M. D. Daniel et al. in Science 228: 1201-1204 (1985), specifically incorporated herein by reference.


[0041] All the SIV proteins, including the envelope, are immune precipitated by sera from HIV-2 infected patients, whereas the serological cross-reactivity of HIV-1 to 2 is restricted to the core proteins. However SIV and HIV-2 can be distinguished by slight differences in the apparent molecular weight of their proteins.


[0042] In terms of nucleotide sequence, it also appears that HIV-2 is closely related to SIV. The genomic RNA of SIV can be detected in stringent conditions as shown in FIG. 2, C by HIV-2 probes corresponding to the LTR and 3′ end of the genome (E2) or to the gag or pol genes. Under the same conditions, HIV-1 derived probes do not detect the SIV genome as shown in FIG. 2, D.


[0043] In Southern blots of DNA from SIV-infected cells, a restriction pattern clearly different from HIV-2ROD and other isolates is seen. All the bands persist after a stringent washing, even though the signal is considerably weakened, indicating a sequence homology throughout the genomes of HIV-2 and SIV. It has recently been shown that baboons and macaques could be infected experimentally by HIV-2, thereby providing an interesting animal model for the study of the HIV infection and its preventive therapy. Indeed, attempts to infect non-human primates with HIV-1 have been successful only in chimpanzees, which are not a convenient model.


[0044] From an initial survey of the restriction maps for certain of the HIV-2 isolates obtained according to the methods described herein, it is already apparent that HIV-2, like HIV-1, undergoes restriction site polymorphism. FIG. 4 A depicts examples of such differences for three isolates, all different one from another and from the cloned HIV-2ROD. It is very likely that these differences at the nucleotide level are accompanied by variations in the amino acid sequence of the viral proteins, as evidenced in the case of HIV-1 and described by M. Alizon et al. in Cell 46: 63-74 (1986), specifically incorporated herein by reference.


[0045] Further, the characterization of HIV-2 will also delineate the domain of the envelope glycoprotein that is responsible for the binding of the surface of the target cells and the subsequent internalization of the virus. This interaction was shown to be mediated by the CD4 molecule itself in the case of HIV-1 and similar studies tend to indicate that HIV-2 uses the same receptor. Thus, although there is wide divergence between the env genes of HIV-1 and 2, small homologous domains of the envelopes of the two HIV could represent a candidate receptor binding site. This site could constitute a target for use to raise a protective immune response against this group of retroviruses.


[0046] From the data discussed herein, certain nucleotide sequences have been identified which are capable of being used as probes in diagnostic methods to obtain the immunological reagents necessary to diagnose an HIV-2 infection. In particular, these sequences may be used as probes in hybridization reactions with the genetic material of infected patients to indicate whether the RNA of the HIV-2 virus is present in these patient's lymphocytes or whether an analogous DNA is present. In this embodiment, the test methods which may be utilized include Northern blots, Southern blots and dot blots. One particular nucleotide sequence which may be useful as a probe is the combination of the 5 kb. HindIII fragment of λROD 4 and the E2 cDNA used in FIG. 4.


[0047] In addition, the genetic sequences of the HIV-2 virus may be used to create the polypeptides encoded by these sequences. Specifically, these polypeptides may be created by expression of the cDNA obtained according to the teachings herein in hosts such as bacteria, yeast or animal cells. These polypeptides may be used in diagnostic tests such as immunofluorescence assays (IFA), radioimmunoassays (RIA) and Western blot tests.


[0048] Moreover, it is also contemplated that additional diagnostic tests, including additional immunodiagnostic tests, may be developed in which the DNA probes or the polypeptides of this invention may serve as one of the diagnostic reagents. The invention described herein includes these additional test methods.


[0049] Thus, it is to be understood that application of the teachings of the present invention to a specific problem or environment will be within the capabilities of one having ordinary skill in the art in light of the teachings contained herein. Examples of the products of the present invention and representative processes for their isolation and manufacture appear above and in the following examples.



EXAMPLES


Example 1

[0050] To prepare DNA probes for use in HIV-2 diagnostic kits, a cDNA complementary to genomic RNA obtained from purified virions was prepared by the following method.


[0051] Supernatant from a 48 hour culture of CEM cells infected with the HIV-2 isolate LAV-II ROD was ultracentrifuged. The virion-containing pellet was then banded on a sucrose gradient and repelleted using substantially the same method as disclosed in U.S. patent application Ser. No. 835,228, supra.


[0052] The purified HIV-2 preparation was used for the synthesis of CDNA through an endogenous, detergent-activated reaction. Briefly, the virion preparation was added to a reaction mixture containing 50 μM Tris-HCl, 5 mM MgCl2, 10 mM DTT, 0.025% Triton, and 50 μM each of the 4 deoxynucleoside triphosphates and oligo (dT) primer. The reaction was carried out for 90 minutes at 37° C.


[0053] After phenol extraction of the proteins present in this first reaction mixture, the second strand of the CDNA was synthesized in the presence of RNAse H, E. coli DNA polymerase I, and four deoxynucleotides for 1 hour at 15° C. and 1 hour at 22° C. Blunt ends were created on this double-stranded cDNA by the action of T4 DNA polymerase. All reagents for this procedure were purchased from Amersham (Amersham cDNA kit) and used as recommended by the supplier.


[0054] After (1) ligation of EcoRI linkers (obtained from Pharmacia) to these blunt ends with T4 DNA ligase (obtained from Biolabs), (2) digestion of the linkers by the restriction endonuclease EcoRI, and (3) elimination of linker fragments by gel filtration on Ultrogel AcA 34 column (LKB-IBF), the cDNA was inserted into a EcoRI-cut Tg130 M13 vector (obtained from Amersham). A cDNA library was obtained after transformation of E. coli TG1 strain. Approximately 104 recombinant M13 plaques were obtained.


[0055] To screen this cDNA library to detect the presence of HIV-2 cDNA-containing recombinant M13 clones, the plaque hybridization technique was used. Briefly, the DNA from M13 plaques was transferred to nitrocellulose filters, and was hybridized to a subgenomic HIV-1 probe derived from clone LAV lambda J19. This probe was comprised of the DNA located between two HindIII restriction sites within the env open reading frame and the R segment of the 3′ LTR. This probe contains the 3′ end of the env gene, the whole F gene, the U3 segment and part of the R segment of the LTR, and is approximately 1500 bp long.


[0056] The probe was obtained by labelling the 1.5 kb HindIII insert with 32P dCTP and dTTP (3000 Ci×10−3 mole) using random primer and Klenow DNA polymerase I in a 4 hour incubation at 15° C. (using a kit obtained from Amersham). The hybridization was carried out overnight in low stringency conditions in a hybridization mixture containing 5×SSC, 5×Denhart solution, 25% formamide, 100 μg/ml denatured salmon sperm DNA, and the labelled probe (2×107 cpm at specificity 109 cpm/μg) at 37° C. The filters were washed out in three successive wash procedures as set forth below. Each wash was followed by autoradiography of the filters.


[0057] Wash #1: 5×SSC, 0.1% SDS, at 25° C. for 4×15 minutes.


[0058] Wash #2: 2×SSC, 0.1% SDS, at 42° C. for 2×30 minutes.


[0059] Wash #3: 0.1×SSC 0.1% SDS, at 65° C. for 2×30 minutes.


[0060] Several positive clones were detected after wash #1, which were still detected after wash #2. However, all signals disappeared after wash #3. This indicated that the positive clones were related only distantly to the genome of HIV-1. These positive clones were picked up, replated, and rehybridized with the same probe in the same stringency conditions as wash #1. The majority of them were found still to be positive.


[0061] The clones were also screened with a total human DNA probe, in medium stringency conditions using the following procedure: hybridization in 5×SSC, 5×Denhart, and 40% formamide followed by washing in 1×SSC, 0.1% SDS, at 50° C. None of the previously positive clones could be detected and therefore did not correspond to repetitive DNA species or CDNA to ribosomal RNA.


[0062] The positive recombinant M13 clones were grown in liquid medium and characterized in different ways:


[0063] (1) Size of their insert: M13 single-stranded template DNA was obtained from each individual clone, and second strand synthesis was conducted with the 17-mer M13 sequence primer and Klenow enzyme. The inserts were excised by EcoRI (Boehringer) and analyzed by electrophoresis on agarose gel. Most inserts were between 600 and 200 bp, except the insert of clone E2.1, which is approximately 2 Kbp long.


[0064] (2) Nucleotide sequence analysis: Several clones have been partially sequenced, using the dideoxy method of Sanger et al. as reported in Proc. Natl. Acad. Sci. 74:5463-7 (1977), specifically incorporated herein by reference. Different independent clones exhibit a similar nucleotide sequence except for the presence of poly-A chains of different length at their 3′ end. This demonstrates that these are cDNA clones from the same RNA template. The detailed sequence analysis of these cDNA clones, which should correspond to the 3′ end of the HIV-2 genome, has shown only a limited homology to HIV-1.


[0065] (3) Hybridization with HIV-2 genomic RNA and DNA


[0066] (a) Obtaining HIV-2 genomic RNA: The infected supernatant was pelleted (50,000 rotations, 30 minutes). The pellet was resuspended in 10 mM Tris pH 7.5, 1 mM EDTA, 0.1% SDS. One of the insert clones F1.1 was labelled, and used as a probe for hybridization with genomic RNA from different viral isolates according to the dot-blot technique. The dot-blot technique consists of the following steps: (i) Spotting the sample (HIV-2 lysate) on a nitrocellulose membrane previously soaked in 20×SS (3M NaCl, 0.3M sodium citrate) and air dried, (ii) baking the membrane for 2 hours at 80° C., and (iii) hybridization. This hybridization was conducted under high stringency conditions (5×SSC, 5×Denhart, 50% formamide at 42° C.), followed by washing in 0.1×SSC, 0.1% SDS at 65° C. Under these conditions, the probe hybridized strongly to spots from two independent isolates of HIV-2, including LAV-II ROD, from which the cloned cDNA was derived. A faint hybridization signal was detected with the spot from STLV-IIImac (Simian T-Lymphotropic Virus (also known as “SIV”) Type III, macaque), and no hybridization was detectable with two isolates of HIV-1.


[0067] Southern blot experiments using E2.1 insert (2 Kb) as a 32P labelled probe did not show detection of hybridization with DNA from uninfected cells but detected band in HIV-2 detached cells in high stringency conditions. HIV-2 shows a polymorphism on the same level of the restriction map as HIV-1. With complete cellular DNA from infected cells, two kinds of signals were detected by Southern blot: (1) in the DNA fraction MW approximately 20 kb and over which is the virus integrated form, and (2) in the fraction of low MW (9,10 kb) which is virus non-integrated in the genome. This pattern is highly characteristic of a retrovirus. Some experiments performed with STLV-III (SIV-3) infected cells showed that the simian retrovirus is distantly related to HIV-2 (the signal was only detected in low stringency conditions). These experiments demonstrate that the probe is specific for HIV-2.


[0068] (4) Subcloning of HIV-2 CDNA into a bacterial plasmid vector: one particular positive M13, E21, was selected, and subcloned into a plasmid vector. This clone has been referred to as pSPE2 and was deposited at the C.N.C.M. in Paris, France, under Accession No. I-595 on Sep. 5, 1986. The DNA of phage M13 (TG 130) recombinant E-2 was purified as a single-stranded DNA of the clone containing 2 kb to the 3′ prime portion of the genome of HIV-2 (LAV-II ROD isolate) already inserted in the phage M-13 (TG130) (obtained from Amersham). This DNA was transferred to the plasmid pSP65, as described by Melton, D. A. in 357 Nucleic Acid Res. 12:035-7056 (1984). Using this technique, the DNA from the M13 recombinant phage was purified and labeled M-13-ROD-E2. In vitro, a second strand was constructed, using 17 mer sequence primer (Amersham), the four nucleotides ACTG, and the DNA polymerase I (Klenow). The EcoRI insert was excised by EcoRI digestion and purified on agarose gel and ligated to pSP65. The pSP65 vector previously was digested with EcoRI. This ligation mixture was used to transform into E. coli DH1 strain, and recombinants were selected for their ability to demonstrate resistance to ampicillin. The identified recombinants were cultured in a LB (Luria medium) containing 50 μg/ml ampicillin and the recombinant plasmids were purified and controlled the presence of the correct insert.



Example 2

[0069] The cDNA obtained from Example 1 was determined to have the following nucleotide sequence:
1        10        20        30 40        50        60        70        80        90       100GTGGAAGGCGAGACTGAAAGCAAGAGGAATACCATTTAGTTAAAGGACAGGAACAGCTATACTTGGTCAGGGCAGGAAGTAACTAACAGAAACAGCTGAG                       MNLI                           ALUI                    MAEIII        PVUII                                                                                             ALUI                                                                                               DDEI       110       120       130       140       150       160       170       180       190       200ACTGCAGGGACTTTCCAGAAGGGGGCTGTAACCAAGGGAGGGACATGGGAGGAGCTGGTGGGGAACGCCTCATATTCTCTGTATAATATACCCGCTGCTTG PSTI                     MAEIII    MNLI  NLAIII   ALUI           MNLI                      BBVI                              STYI             MNLI                                         FNU4HI                                                                                              TTHllllII       210       220       230       240       250       260       270 280       290       300CATTGTACTTCAGTCGCTCTGCGGAGAGGCTGGCAGATTGAGCCCTGGAGGATCTCTCCAGCACTAGACGGATGAGCCTGGGTGCCCTGCTAGACTCTCA    RSAI                 MNLI          BANII   MNLI            MAEI         APYI BSP1286 MAEI    HPHI                                       BSP1286   XHOII                      BSTMI           HINFI                                          APYI    DPNI                      ECORII                                          BSTNI   MBOI                      SCRFI                                          ECORII  NDEII                         BANI                                          SCRFI   SAUIIIA       310       320       330       340       350       360       370       380CCAGCACTTGGCCGGTGCTGGCAGACGGCCCCACGCTTGCCTGCTTAAAAACCTTCCTTAATAAAGCTGCAGTAGAAGCA         HAEIII           HAEIII                                ALUI           HAPII          SAU96A                                 BBVI           HPAII                                                   FNU4HI           MSPI



Example 3

[0070] Cloning of a cDNA Complementary to Genomic RNA from HIV-2 Virions


[0071] HIV-2 virions were purified from 5 liters of supernatant from a culture of the CEM cell line infected with the ROD isolate and a CDNA first strand using oligo (dT) primer was synthesized in detergent activated endogenous reaction on pelleted virus, as described by M. Alizon et al. in Nature, 312: 757-760 (1984), specifically incorporated herein by reference. RNA-cDNA hybrids were purified by phenol-chloroform extraction and ethanol precipitation. The second-strand cDNA was created by the DNA polymerase I/RNAase H method of Sanger et al. described in Proc. Nat'l. Acad. Sci. USA, 74: 5463-5467 (1977), specifically incorporated herein by reference. The double-stranded cDNA was blunt ended with T4 DNA polymerase using a commercial cDNA synthesis kit (obtained from Amersham). After attachment of EcoRI linkers (obtained from Pharmacia), EcoRI digestion, and ligation into EcoRI-digested dephosphorylated M13 tg 130 vector (obtained from Amersham), a cDNA library was obtained by transformation of the E. coli TG1 strain. Recombinant plaques (104) were screened in situ on replica filters with the 1.5 kb. HindIII fragment from clone J19, corresponding to the 3′ part of the genome of the LAVBRU isolate of HIV-1, 32P labelled to a specific activity of 109 cpm/μg. The filters were prehybridized in 5×SSC, 5×Denhardt solution, 25% formamide, and denatured salmon sperm DNA (100 μg/ ml) at 37° C. for 4 hours and hybridized for 16 hours in the same buffer (Tm −42° C.) plus 4×107 cpm of the labelled probe (106 cpm/ml of hybridization buffer). The washing was done in 5×SSC, 0.1% SDS at 25° C. for 2 hours. 20×SSC is 3M NaCl, 0.3M Na citrate. Positive plaques were purified and single-stranded M13 DNA prepared and end-sequenced according to the method of Sanger et al., supra.



Example 4

[0072] Hybridization of DNA from HIV-1 and HIV-2 Infected Cells and RNA from HIV-1 and 2 and SIV Virions with a Probe Derived from an HIV-2 Cloned cDNA


[0073] DNA was extracted from infected CEM cells continuously producing HIV-1 or 2. The DNA digested with 20 μg of PstI, or undigested, was electrophoresed on a 0.8% agarose gel, and Southern-transferred to nylon membrane. Virion dot-blots were prepared in duplicate, as described by F. Clavel et al. in Science 233: 343-346 (1986), specifically incorporated herein by reference, by pelleting volumes of supernatant corresponding to the same amount of reverse transcriptase activity. Prehybridization was done in 50% formamide, 5×SSC, 5×Denhardt solution, and 100 mg/ml denatured salmon sperm DNA for 4 hours at 42° C. Hybridization was performed in the same buffer plus 10% Dextran sulphate, and 106 cpm/ml of the labelled E2 insert (specific activity 109 cpm/μg) for 16 hours at 42° C. Washing was in 0.1×SSC, 0.1% SDS for 2×30 min. After exposure for 16 hours with intensifying screens, the Southern blot was dehybridized in 0.4 N NaOH, neutralized, and rehybridized in the same conditions to the HIV-1 probe labelled to 109 cpm/μg.



Example 5

[0074] Cloning in Lambda Phage of the Complete Provirus DNA of HIV-2


[0075] DNA from the HIV-2ROD infected CEM (FIG. 2, lanes a and c) was partially digested with Sau3AI. The 9-15 kb fraction was selected on a 5-40% sucrose gradient and ligated to BamHI arms of the lambda L47.1 vector. Plaques (2×106) obtained after in vitro packaging and plating on E. coli LA 101 strain were screened in situ with the insert from the E2 CDNA clone. Approximately 10 positive clones were plaque purified and propagated on E. coli C600 recBC. The λROD 4, 27, and 35 clones were amplified and their DNA characterized by restriction mapping and Southern blotting with the HIV-2 cDNA clone under stringent conditions, and gag-pol probes from HIV-1 used under non stringent conditions.


[0076] It will be apparent to those skilled in the art that various modifications and variations can be made in the processes and products of the present invention. Thus, it is intended that the present application cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents.


Claims
  • 1. A method for the diagnosis of an HIV-2 infection comprising the steps of: (a) contacting DNA or RNA from a body sample suspected of containing viral genetic material with a detectable complementary DNA probe in a hybridization solution to form a mixture of nucleic acids, (b) washing the mixture of nucleic acids with a wash solution, and (c) detecting the formation of a hybridized complex, wherein steps (a) and (b) are performed under conditions that allow generation of a strong hybridization signal in the presence of genomic RNA of HIV-2 and a faint hybridization signal in the presence of genomic RNA of HIV-1, wherein said detectable, complementary DNA probe is such that (a) hybridization of the DNA probe with nucleic acids of HIV-2 under hybridization conditions can be strongly detected, (b) hybridization of the DNA probe with nucleic acids of STLV-IIImac under hybridization conditions can be faintly detected, and (c) hybridization of the DNA probe with nucleic acids of HIV-1 under hybridization conditions cannot be detected; and further wherein said hybridization conditions comprise contacting DNA probe with said HIV-2, STLV-IIImac, or HIV-1 in a hybridization solution consisting essentially of 5×SSC, 5×Denhart, and 50% formamide at 42° C. followed by washing with a wash solution consisting essentially of 0.1×SSC and 0.1% SDS at 64° C.
  • 2. The method of claim 1 wherein step b is performed by a process selected from the group consisting of Southern blot, Northern blot and dot blot.
  • 3. The method of claim 1 wherein the complementary DNA comprises plasmid pSPE2 in clone CNCM No. I-595.
  • 4. The method of claim 1 wherein a part of the probe is complementary to the U3 region of the HIV-2 genome.
  • 5. The method of claim 1 wherein a part of the probe is complementary to the total R region of the HIV-2 genome.
  • 6. A process for detecting the presence of HIV-2 comprising: (a) providing a sample suspected of containing viral genetic material; (b) contacting said sample with a DNA probe; and (c) determining whether a hybridized complex is formed, wherein said DNA probe is capable of producing a strong hybridization signal in the presence of genomic RNA of HIV-2, a weak hybridization signal in the presence of genomic RNA of SIV and faint or no hybridization signal in the presence of genomic RNA of HIV-1.
  • 7. A method for the diagnosis of an HIV-2 infection comprising the steps of: (a) contacting DNA or RNA from a body sample of a person suspected of having an HIV-2 infection with a cDNA probe under conditions sufficient to form a detectable hybridized complex in the presence of an HIV-2 infection; and (b) determining whether said hybridized complex is formed, wherein said CDNA probe comprises a nucleotide sequence that is substantially complementary to a HIV-2 genomic RNA, said all or part of the nucleotide sequence is capable of specifically detecting the presence of HIV-2 and said nucleotide sequence comprises 2        10        20        30        40        50        60        70        80        90       100GTGGAAGGCGAGACTGAAAGCAAGAGGAATACCATTTAGTTAAAGGACAGGAACAGCTATACTTGGTCAGGGCAGGAAGTAACTAACAGAAACAGCTGAG                       MNLI                           ALUI                    MAEIII        PVUII                                                                                             ALUI                                                                                               DDEI       110       120       130       140       150       160       170       180       190       200ACTGCAGGGACTTTCCAGAAGGGGCTGTAACCAAGGGAGGGACATGGGAGGAGCTGGTGGGGAACGCCTCATATTCTCTGTATAATATACCCGCTGCTTG PSTI                     MAEIII    MNLI  NLAIII   ALUI           MNLI                      BBVI                              STYI             MNLI                                         FNU4HI                                                                                              TTHIIIIII       210       220       230       240       250       260       270       280       290       300CATTGTACTTCAGTCGCTCTGCGGAGAGGCTGGCAGATTGAGCCCTGGAGGATCTCTCCAGCACTAGACGGATGAGCCTGGGTGCCCTGCTAGACTCTCA    RSAI                 MNLI          BANII   MNLI            MAEI         APYI BSP1286 MAEI   HPHI                                       BSP1286   XHOII                      BSTNI           HINFI                                          APYI    DPNI                      ECORII                                          BSTNI   MBOI                      SCRFI                                          ECORII  NDEII                         BANI                                                 SCRFI   SAUIIIA       310       320       330       340       350       360       370       380CCAGCACTTGGAAGGTGCTGGCAGACGGCCCCACGCTTGCCTGCTTAAAAACCTTCCTTAATAAAGCTGCAGTAGAAGCA         HAEIII           HAEIII                                ALUI           HAPII          SAU96A                                 BBVI           HPAII                                                 FNU4HI           MSPI
  • 8. A DNA probe capable of hybridizing under high stringency conditions to all or part of a viral RNA genome or proviral DNA genome of HIV-2 virus to form a hybridized complex, wherein said hybridized complex is capable of being detected, and wherein said high stringency conditions comprise a hybridization condition and a wash condition that allow generation of a strong hybridization signal in the presence of genomic RNA of HIV-2, a weak hybridization signal in the presence of genomic RNA of SIV and a faint or no hybridization signal in the presence of genomic RNA of HIV-1.
  • 9. A DNA probe as claimed in claim 8, wherein said portion of the genome of the HIV-2 virus comprises the total R region of the HIV-2 genome.
  • 10. A DNA probe as claimed in claim 8, wherein said portion of the genome of the HIV-2 virus comprises the U3 region of the HIV-2 genome.
  • 11. A DNA probe as claimed in claim 8, wherein the cDNA probe comprises a sequence derived from pSPE2.
  • 12. The DNA probe of claim 8, wherein the DNA probe comprises all or part of a viral DNA having the identifying characteristics of viral DNA deposited under culture collection accession number C.N.C.M. No. I-626.
  • 13. The DNA probe of claim 8, wherein the DNA probe comprises all or part of a viral DNA having the identifying characteristics of viral DNA deposited under culture collection accession number C.N.C.M. No. 1-627.
  • 14. The DNA probe of claim 8, wherein the DNA probe comprises all or part of a viral DNA having the identifying characteristics of viral DNA deposited under culture collection accession number C.N.C.M. No. I-628.
  • 15. A DNA probe as claimed in claim 8, wherein said probe is capable of hybridizing to an entire viral RNA genome or proviral DNA genome.
Priority Claims (4)
Number Date Country Kind
86 00910 Jan 1986 FR
86 00911 Jan 1986 FR
86 01635 Feb 1986 FR
86 01985 Feb 1986 FR
Parent Case Info

[0001] This application is a continuation of U.S. patent application Ser. No. 07/602,383 of Montagnier et al. for “Cloned DNA Sequences Related to the Genomic RNA of the Human Immunodeficiency Virus II (HIV-II), Polypeptides Encoded by these DNA Sequences and Use of these DNA Clones and Polypeptides in Diagnostic Kits,” filed Oct. 24, 1990, which is a continuation of U.S. patent application Ser. No. 06/916,080, filed Oct. 6, 1986, now abandoned, which is a continuation-in-part of U.S. patent application Ser. No. 06/835,228 of Montagnier et al. for “New Retrovirus Capable of Causing AIDS, Antigens Obtained from this Retrovirus and Corresponding Antibodies and their Application for Diagnostic Purposes,” filed Mar. 3, 1986, now U.S. Pat. No. 4,839,288. This application is also a continuation of U.S. patent application Ser. No. 07/604,323 of Alizon et al. for “Cloned DNA Sequences Related to the Entire Genomic RNA of Human Immunodeficiency Virus (HIV-II), Polypeptides Encoded by these DNA Sequences and Use of these DNA Clones and Polypeptides in Diagnostic Kits,” filed Oct. 24, 1990, which is a continuation of U.S. patent application Ser. No. 06/933,184, filed Nov. 24, 1986, now abandoned, which is a continuation-in-part of U.S. patent application Ser. No. 06/916,080, filed Oct. 6, 1986, now abandoned, which is a continuation-in-part of U.S. patent application Ser. No. 06/835,228, filed Mar. 3, 1986, now U.S. Pat. No. 4,839,288. The contents of all applications and patents are incorporated herein by reference.

Continuations (6)
Number Date Country
Parent 08467161 Jun 1995 US
Child 09986634 Nov 2001 US
Parent 08132919 Oct 1993 US
Child 08467161 Jun 1995 US
Parent 07756998 Sep 1991 US
Child 08132919 Oct 1993 US
Parent 07602383 Oct 1990 US
Child 07756998 Sep 1991 US
Parent 06916080 Oct 1986 US
Child 07602383 Oct 1990 US
Parent 06933184 Nov 1986 US
Child 07604323 Oct 1990 US
Continuation in Parts (3)
Number Date Country
Parent 06835228 Mar 1986 US
Child 06916080 Oct 1986 US
Parent 06916080 Oct 1986 US
Child 06933184 Nov 1986 US
Parent 06835228 Mar 1986 US
Child 06916080 Oct 1986 US