Claims
- 1. A method for determining the presence of autoantibodies to human islet cell glutamic acid decarboxylase (GAD) in a biological sample, the method comprising:
- contacting the biological sample with a recombinant human islet cell GAD polypeptide free of other human proteins and produced by a cell transformed or transfected with a construct for expressing said GAD polypeptide, which construct comprises a DNA sequence encoding said GAD polypeptide wherein said sequence comprises the nucleotide sequence of FIG. 2, SEQ ID No. 1, from nucleotide 38 to nucleotide 1792, or a nucleotide sequence degenerate thereto which codes for the GAD polypeptide encoded by said nucleotide sequence of FIG. 2, SEQ ID No. 1, from nucleotide 38 to nucleotide 1792, wherein the polypeptide catalyzes the synthesis of .gamma.-aminobutyric acid, and
- detecting the presence of immune complex formation between said GAD polypeptide and autoantibodies to human islet cell GAD and therefrom determining the presence of autoantibodies to human islet cell GAD in the sample.
- 2. The method of claim 1, wherein the GAD polypeptide is attached to a solid phase support.
- 3. The method of claim 1, wherein the GAD polypeptide is labeled.
- 4. The method of claim 1, wherein said immune complex is detected by a second antibody.
- 5. The method of claim 1, wherein said detecting step is by enzyme reaction, fluorescence, luminescence, or radioactivity.
- 6. The method of claim 1, wherein the biological sample is blood, plasma, serum, or urine.
RELATED CASES
This application is a continuation of U.S. application Ser. No. 07/883,492, filed May 15, 1992, now abandoned, which is a continuation of U.S. application Ser. No. 07/702,162, filed May 15, 1991, which is incorporated herein by reference.
GOVERNMENT SUPPORT
This invention was made with government support under grant numbers DK26190, DK33873, and DK41801 awarded by the National Institutes of Health/Juvenile Diabetes Foundation. The government has certain rights in the invention.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4952507 |
Woodward |
Aug 1990 |
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Foreign Referenced Citations (1)
Number |
Date |
Country |
383129 |
Aug 1990 |
EPX |
Continuations (2)
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Number |
Date |
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Parent |
883492 |
May 1992 |
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Parent |
702162 |
May 1991 |
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