Claims
- 1. A method for producing a human GM-CSF protein having higher specific activity than native human GM-CSF, comprising:
- introducing into a eucaryotic host cell an expression unit comprising a promoter followed downstream by a DNA sequence encoding a human GM-CSF protein lacking N-linked glycosylation but having O-linked glycosylation, said protein having higher specific activity than native human GM-CSF;
- growing said eucaryotic host cell in an appropriate medium; and
- isolating the protein product encoded by said DNA sequence and produced by said eucaryotic host cell.
- 2. A method according to claim 1 wherein said host cell is a Saccharomyces cerevisiae cell.
- 3. A method according to claim 1 wherein said protein comprises the amino acid sequence of FIG. 1, starting with alanine, number 18, and ending with glutamate, number 144.
- 4. A method for producing a human GM-CSF protein having higher specific activity than native human GM-CSF, comprising:
- introducing into a eucaryotic host cell an expression unit comprising a promoter followed downstream by a DNA sequence encoding a human GM-CSF protein lacking N-linked and O-linked glycosylation, said protein having higher specific activity than native human GM-CSF;
- growing said eucaryotic host cell in an appropriate medium; and
- isolating the protein product encoded by said DNA sequence and produced by said eucaryotic host cell.
- 5. A method according to claim 4 wherein said protein comprises the amino acid sequence of FIG. 2, starting with alanine, number 18, and ending with glutamate, number 144.
- 6. A method according to claim 4 wherein said host cell is a Saccharomyces cerevisiae cell.
CROSS REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of U.S. Ser. No. 07/008,698, filed Jan. 29, 1987, now abandoned.
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5032676 |
Deeley et al. |
Jul 1991 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
08698 |
Jan 1987 |
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